Starting /dee2/code/volunteer_pipeline.sh SRR13165384 current disk space = 1541434626048 free memory = 1602367720 SRR13165384 SRAfilesize bec2433ab35be87cad5263db77d932a2 SRR13165384.sra SRR13165384.sra file validated SRR13165384 is paired end SRR13165384 is conventional basespace SRR13165384 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165384_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5565 37.0 37.0 37.0 37.0 37.0 2 36.1615 37.0 37.0 37.0 37.0 37.0 3 36.4875 37.0 37.0 37.0 37.0 37.0 4 36.5625 37.0 37.0 37.0 37.0 37.0 5 36.518 37.0 37.0 37.0 37.0 37.0 6 36.619 37.0 37.0 37.0 37.0 37.0 7 36.47 37.0 37.0 37.0 37.0 37.0 8 36.591 37.0 37.0 37.0 37.0 37.0 9 36.4255 37.0 37.0 37.0 37.0 37.0 10-14 36.5462 37.0 37.0 37.0 37.0 37.0 15-19 36.4892 37.0 37.0 37.0 37.0 37.0 20-24 36.51389999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.3776 37.0 37.0 37.0 37.0 37.0 30-34 36.409000000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.409 37.0 37.0 37.0 37.0 37.0 40-44 36.38250000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.359899999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.2998 37.0 37.0 37.0 37.0 37.0 55-59 36.322599999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.2746 37.0 37.0 37.0 37.0 37.0 65-69 36.235 37.0 37.0 37.0 37.0 37.0 70-74 36.2573 37.0 37.0 37.0 37.0 37.0 75-79 36.201800000000006 37.0 37.0 37.0 37.0 37.0 80-84 36.1699 37.0 37.0 37.0 37.0 37.0 85-89 36.1665 37.0 37.0 37.0 37.0 37.0 90-94 36.150800000000004 37.0 37.0 37.0 37.0 37.0 95-99 36.140499999999996 37.0 37.0 37.0 37.0 37.0 100-104 36.1335 37.0 37.0 37.0 37.0 37.0 105-109 36.1395 37.0 37.0 37.0 37.0 37.0 110-114 36.1042 37.0 37.0 37.0 37.0 37.0 115-119 36.0484 37.0 37.0 37.0 37.0 37.0 120-124 35.936400000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.975 37.0 37.0 37.0 37.0 37.0 130-134 35.8936 37.0 37.0 37.0 37.0 37.0 135-139 35.720299999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.603100000000005 37.0 37.0 37.0 37.0 37.0 145-149 35.4074 37.0 37.0 37.0 37.0 37.0 150-151 35.26375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 0.0 23 4.0 24 3.0 25 4.0 26 5.0 27 7.0 28 19.0 29 26.0 30 24.0 31 37.0 32 46.0 33 100.0 34 132.0 35 350.0 36 2803.0 37 438.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.800000000000004 12.625 5.225 36.35 2 23.15047810770005 11.17262204328133 33.064921992954204 32.611977856064414 3 21.175 14.399999999999999 26.474999999999998 37.95 4 26.075 20.0 21.6 32.324999999999996 5 26.875 27.35 21.025 24.75 6 24.025 29.725 23.05 23.200000000000003 7 18.875 26.474999999999998 36.55 18.099999999999998 8 20.0 24.8 29.275000000000002 25.924999999999997 9 19.625 20.95 34.875 24.55 10-14 22.395 25.915 26.365 25.324999999999996 15-19 23.625 24.975 26.125 25.275 20-24 22.634999999999998 26.395000000000003 25.619999999999997 25.35 25-29 24.21 25.345000000000002 25.235000000000003 25.21 30-34 24.065 25.405 25.685000000000002 24.845 35-39 23.044999999999998 25.185000000000002 25.124999999999996 26.645000000000003 40-44 22.285 25.105 26.22 26.39 45-49 23.565 25.974999999999998 24.805 25.655 50-54 23.815 25.785000000000004 24.545 25.855 55-59 24.005000000000003 25.180000000000003 24.97 25.845000000000002 60-64 22.725 25.14 25.295 26.840000000000003 65-69 22.900000000000002 26.26 24.73 26.11 70-74 23.845 25.56 25.495 25.1 75-79 23.66 25.455 25.285000000000004 25.6 80-84 23.95 25.14 24.745 26.165 85-89 24.19 25.385 24.925 25.5 90-94 23.474999999999998 26.119999999999997 24.395 26.009999999999998 95-99 24.715 25.685000000000002 24.525 25.074999999999996 100-104 24.285 26.195 24.05 25.47 105-109 23.805 25.724999999999998 24.255 26.215 110-114 24.47 25.69 24.38 25.46 115-119 24.635 25.615 24.055 25.695 120-124 23.435 25.474999999999998 24.245 26.845000000000002 125-129 24.32 25.759999999999998 23.895 26.025 130-134 24.25 25.885 23.765 26.1 135-139 23.625 26.195 24.275 25.905 140-144 23.674999999999997 25.955000000000002 23.56 26.810000000000002 145-149 23.61 25.22 23.96 27.21 150-151 23.6875 25.087500000000002 24.4125 26.8125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 0.5 28 1.0 29 3.0 30 4.0 31 3.5 32 13.5 33 29.5 34 29.5 35 32.5 36 50.0 37 72.0 38 82.5 39 105.5 40 128.0 41 147.0 42 178.5 43 188.5 44 191.5 45 194.5 46 185.0 47 191.5 48 186.0 49 173.5 50 178.5 51 159.5 52 132.0 53 128.0 54 115.0 55 96.5 56 89.0 57 85.0 58 81.0 59 65.5 60 60.5 61 55.5 62 53.5 63 49.5 64 43.5 65 47.0 66 42.0 67 41.5 68 45.0 69 45.0 70 37.5 71 31.0 72 34.0 73 29.0 74 17.0 75 9.0 76 10.5 77 9.0 78 6.0 79 4.0 80 2.0 81 1.5 82 2.0 83 1.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.65 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.85 #Duplication Level Percentage of deduplicated Percentage of total 1 71.53231663035584 49.25 2 18.40958605664488 25.35 3 6.209150326797386 12.825000000000001 4 2.287581699346405 6.3 5 0.7262164124909223 2.5 6 0.6172839506172839 2.55 7 0.07262164124909223 0.35000000000000003 8 0.07262164124909223 0.4 9 0.03631082062454612 0.22499999999999998 >10 0.03631082062454612 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTCTCGATCTCCCTCCATATCTGATCCATGGAATATCCATTCACGACAC 10 0.25 No Hit GGTGTAGCTTCATTGCGTGGAGTGAGTGATAGCAAGCTTGTTTATTATGG 9 0.22499999999999998 No Hit GGGGCAGATGCTTTAGGCCAACAAGCCAAATGTGTTCTCGCCACTGTAAG 8 0.2 No Hit CGGGCAGTGACCTGACGCAAAATTAGGAGAGGCACTCGTGGAAGAAGACT 8 0.2 No Hit GCTGGGAGCAGCTGGGATGGGTATCGGCCCCGGGTTCAGGGTCCGGAACC 7 0.17500000000000002 No Hit AGCTGCGATCCTTCCTTCACGACCGGCAAGTCTGTCCTCACCCGTCCACC 7 0.17500000000000002 No Hit GTGGACTCCTTCTGGATGTTGTAGTCCGCAAGGGTCCTGCCATCTTCAAG 6 0.15 No Hit CTCGAGAGTACTTCACAGCAGCAAGCGGCATGCACAGCTCTTCAGCAAGC 6 0.15 No Hit GGCAGCAATTTCTAATCTTCACTGGACAACTTGTGGGTCCTAATTGGGCC 6 0.15 No Hit GTTCTGGTTCTGCATTTCATCAAGCCGAGTAAGTGAAGTGTTGGCGCCTG 6 0.15 No Hit CAGCAGCCATCAAATCCTCGTACTTTCCTGTAGCGGCATTGTAACCATAC 6 0.15 No Hit ATGCCATCATCATCATCGTCCCAAGTGTCAGGCTTTTTGTCCTTTGGATC 6 0.15 No Hit CACCATTACAACCAAAGCCCCTGTGCCACTCCTTATGGTCATGCTCTGTC 6 0.15 No Hit CTGCATTGGAATGTTACCAGGATTCATCCCAACCATGCCCGGTTGGGGCA 6 0.15 No Hit CAGCATAAGAGGACCTTCGGGGTCACAGTTTCTAATAGCGGTTGCATATA 6 0.15 No Hit GGCACAAATGATAAATGAACTGCCCAATAATAAATAGAAGTACATATCAT 6 0.15 No Hit CGAGCAGTTCCATTTTGATTCAACTCTGAACCGGCACCCCCTTAATAAAA 6 0.15 No Hit CTCGAGTCCGTATTTTGCTTCTCTTCACCAGCATTAGCACCCTTTGTTGC 6 0.15 No Hit GTGCAGAACATCAAAATTTCTTCAAGTTGCAACTTACAAGCATTTCTTCC 6 0.15 No Hit GTTCAGATATTTGTGTGATGATGATGCTCCTCTACAGAATTGTATAACAG 6 0.15 No Hit CTGCTCATCCCACCGGCTGAAGAGAGAATTGGTCACATAAACCCTCTTGC 6 0.15 No Hit CACCGTCATCCTCTTCTTCATCATCACTATATTGCCCAAGCAATGCCAGA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCCGAAATCTCGTAT 6 0.15 TruSeq Adapter, Index 10 (97% over 37bp) GTCATAATCATTTGGCAAGAAGACTTCAGTTTAAACTGACATATTGCGTA 5 0.125 No Hit GTGGCATCCAACAGCAGCAGGTCCTGGACCTTCTCATTGCCCTCTCCGCT 5 0.125 No Hit ATCTGAAGTTTGTCAGTGTCACTTTTCTCATGTTACAGGGAGTTACCATC 5 0.125 No Hit ACTAAGACCTGGCTCAGTAAGCACAATGTCTGAGGCACTTCTAGCAGCAT 5 0.125 No Hit CGGCCTTCTTCTTGGTGTCAAAGAGGTCAAAGGGGGCCCTCTTGGGGACA 5 0.125 No Hit GGCCCGATAATGCCTAGTAGAAACCACATAGGTCTGGTCCTGCTGGTCAA 5 0.125 No Hit ATCACAAATCACAAGGTCACCACCATCAAAGCAAATGAAGCAGACCTCCT 5 0.125 No Hit AGTTAAACGTAATTGAACAAAATGAAGGCAATGAAAGCAATACCTCTGGA 5 0.125 No Hit CACTGTTGTCAATATCAGCCGCATCCATTAAAGACTGGATTTCAGGTTCC 5 0.125 No Hit GCTGCGTCGGAGATGGCGACGACGGTGGAGTCATCGCCTTCGTAGGCGTC 5 0.125 No Hit TTTTTTTTTTTGTTATAGTGAACCATTGCTGATTACTATTACTGATTGGA 5 0.125 No Hit GGCATTCTTACATCCTTATTCGGCGACTATTACATTCTTATTCAGTGACT 5 0.125 No Hit AGTAGATGTCATTCTGGCCCTCCTTCATCCTGGTCACATAGTCCTTGAGG 5 0.125 No Hit AGTGATAACAGCTTGGCTCCCCACTACTGCTGCGAGAATGGCGATCGCGA 5 0.125 No Hit ACAGATATTCATGTAGGTTATGGTCCAAAGTAATACCGTCTTGATGTCAC 5 0.125 No Hit CCCACGATCAGGCCATAGAGAGCAAGAGCTTCTGCGAAGATAAGGATGAG 5 0.125 No Hit GACCACATAAGTTTTTCTCACGAAAACGACTCCAAACATACTTACAGCCA 5 0.125 No Hit ACCACCAGCAAGCTTTGCAATCCTCTCATTGAGTTTTTCCTTTTCATAAT 5 0.125 No Hit CAGCATTTGGTACTCCTTATTGTTTACATGGCATACACAATATTTTACCT 5 0.125 No Hit GGTGCCTGTATGATCAGAGTGCTTGGAGCGAGCTCAAGCCTGTGGCTTAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.2125 0.0 0.0 0.0 0.0 76-77 0.3375 0.0 0.0 0.0 0.0 78-79 0.44999999999999996 0.0 0.0 0.0 0.0 80-81 0.5 0.0 0.0 0.0 0.0 82-83 0.525 0.0 0.0 0.0 0.0 84-85 0.7125 0.0 0.0 0.0 0.0 86-87 0.9875 0.0 0.0 0.0 0.0 88-89 1.2875 0.0 0.0 0.0 0.0 90-91 1.5625 0.0 0.0 0.0 0.0 92-93 2.0125 0.0 0.0 0.0 0.0 94-95 2.2875 0.0 0.0 0.0 0.0 96-97 2.7249999999999996 0.0 0.0 0.0 0.0 98-99 3.225 0.0 0.0 0.0 0.0 100-101 3.55 0.0 0.0 0.0 0.0 102-103 3.8875 0.0 0.0 0.0 0.0 104-105 4.475 0.0 0.0 0.0 0.0 106-107 4.8375 0.0 0.0 0.0 0.0 108-109 5.35 0.0 0.0 0.0 0.0 110-111 5.75 0.0 0.0 0.0 0.0 112-113 6.475 0.0 0.0 0.0 0.0 114-115 7.012499999999999 0.0 0.0 0.0 0.0 116-117 7.5375 0.0 0.0 0.0 0.0 118-119 8.3375 0.0 0.0 0.0 0.0 120-121 9.25 0.0 0.0 0.0 0.0 122-123 10.0375 0.0 0.0 0.0 0.0 124-125 10.675 0.0 0.0 0.0 0.0 126-127 11.2625 0.0 0.0 0.0 0.0 128-129 12.149999999999999 0.0 0.0 0.0 0.0 130-131 12.9125 0.0 0.0 0.0 0.0 132-133 13.850000000000001 0.0 0.0 0.0 0.0 134-135 14.625 0.0 0.0 0.0 0.0 136-137 15.7375 0.0 0.0 0.0 0.0 138-139 16.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAATC 10 0.006830828 145.0 2 TTCTTCG 20 0.00593511 29.0 15-19 >>END_MODULE SRR13165384 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165384_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.15875 37.0 37.0 37.0 37.0 37.0 2 36.3095 37.0 37.0 37.0 37.0 37.0 3 36.1625 37.0 37.0 37.0 37.0 37.0 4 36.301 37.0 37.0 37.0 37.0 37.0 5 36.374 37.0 37.0 37.0 37.0 37.0 6 36.314 37.0 37.0 37.0 37.0 37.0 7 36.226 37.0 37.0 37.0 37.0 37.0 8 36.233 37.0 37.0 37.0 37.0 37.0 9 36.342 37.0 37.0 37.0 37.0 37.0 10-14 36.2596 37.0 37.0 37.0 37.0 37.0 15-19 36.263400000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.2168 37.0 37.0 37.0 37.0 37.0 25-29 36.1778 37.0 37.0 37.0 37.0 37.0 30-34 36.12820000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.0988 37.0 37.0 37.0 37.0 37.0 40-44 36.1201 37.0 37.0 37.0 37.0 37.0 45-49 36.082499999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.07189999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.0295 37.0 37.0 37.0 37.0 37.0 60-64 36.0236 37.0 37.0 37.0 37.0 37.0 65-69 36.036500000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.930099999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.948800000000006 37.0 37.0 37.0 37.0 37.0 80-84 35.992599999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.8218 37.0 37.0 37.0 37.0 37.0 90-94 35.895500000000006 37.0 37.0 37.0 37.0 37.0 95-99 35.8751 37.0 37.0 37.0 37.0 37.0 100-104 35.8507 37.0 37.0 37.0 37.0 37.0 105-109 35.8424 37.0 37.0 37.0 37.0 37.0 110-114 35.789100000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.65 37.0 37.0 37.0 37.0 37.0 120-124 35.607299999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.5495 37.0 37.0 37.0 37.0 37.0 130-134 35.341300000000004 37.0 37.0 37.0 34.6 37.0 135-139 35.196450000000006 37.0 37.0 37.0 32.2 37.0 140-144 35.08710000000001 37.0 37.0 37.0 32.2 37.0 145-149 34.87665 37.0 37.0 37.0 25.0 37.0 150-151 34.633250000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 5.0 14 2.0 15 2.0 16 1.0 17 3.0 18 3.0 19 1.0 20 4.0 21 3.0 22 5.0 23 6.0 24 1.0 25 6.0 26 7.0 27 14.0 28 13.0 29 21.0 30 18.0 31 41.0 32 63.0 33 113.0 34 225.0 35 515.0 36 2661.0 37 266.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.40305994482067 22.67368949084525 9.907198394783045 28.016052169551042 2 30.599999999999998 23.549999999999997 26.400000000000002 19.45 3 24.025 25.5 28.95 21.525 4 27.400000000000002 30.175 21.2 21.224999999999998 5 26.924999999999997 35.125 18.925 19.025 6 23.474999999999998 36.0 19.025 21.5 7 21.375 20.275000000000002 35.5 22.85 8 23.75 22.7 25.674999999999997 27.875 9 24.025 22.75 26.3 26.924999999999997 10-14 26.33 26.365 23.43 23.875 15-19 26.14 25.259999999999998 24.560000000000002 24.04 20-24 24.925 25.27 25.05 24.755 25-29 26.465 24.715 24.595 24.224999999999998 30-34 26.484999999999996 25.474999999999998 24.415 23.625 35-39 25.835 24.845 24.79 24.529999999999998 40-44 25.779999999999998 24.715 25.124999999999996 24.38 45-49 25.345000000000002 25.1 25.0 24.555 50-54 26.179999999999996 24.73 25.180000000000003 23.91 55-59 26.145000000000003 25.025 24.82 24.01 60-64 26.77 25.430000000000003 24.535 23.265 65-69 26.41 25.335 24.560000000000002 23.695 70-74 25.674999999999997 25.415 24.9 24.01 75-79 26.26 25.445 24.48 23.815 80-84 26.08 25.324999999999996 24.035 24.560000000000002 85-89 26.255 25.419999999999998 24.975 23.35 90-94 27.125 25.385 24.39 23.1 95-99 26.179999999999996 25.845000000000002 24.075 23.9 100-104 26.735 24.73 24.490000000000002 24.044999999999998 105-109 26.985 24.515 24.92 23.580000000000002 110-114 27.589999999999996 24.79 24.099999999999998 23.52 115-119 28.105000000000004 24.81 24.63 22.455 120-124 28.084999999999997 26.105 23.425 22.384999999999998 125-129 27.700000000000003 26.490000000000002 23.505000000000003 22.305 130-134 28.76787678767877 26.572657265726573 23.13231323132313 21.527152715271527 135-139 29.24146207310366 26.021301065053255 23.701185059252964 21.03605180259013 140-144 30.78 25.615 22.975 20.630000000000003 145-149 29.721486074303716 25.6262813140657 23.311165558277914 21.341067053352667 150-151 31.24531132783196 24.318579644911228 24.3935983995999 20.042510627656913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 1.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 1.5 24 3.0 25 2.0 26 1.0 27 1.0 28 3.0 29 5.0 30 4.5 31 6.5 32 13.0 33 15.5 34 27.0 35 38.5 36 45.0 37 55.5 38 67.5 39 107.0 40 130.5 41 124.5 42 149.0 43 175.5 44 200.0 45 223.0 46 206.5 47 197.5 48 181.5 49 168.5 50 149.0 51 146.5 52 142.0 53 108.0 54 117.5 55 104.0 56 79.5 57 94.0 58 86.0 59 64.0 60 59.5 61 62.0 62 61.0 63 59.0 64 69.0 65 58.0 66 38.0 67 41.0 68 49.5 69 42.0 70 30.5 71 30.0 72 30.0 73 24.5 74 23.0 75 20.0 76 12.5 77 9.5 78 8.5 79 4.5 80 2.0 81 3.5 82 3.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.5 94 1.0 95 1.5 96 2.0 97 1.5 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.01 135-139 0.005 140-144 0.0 145-149 0.005 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.72500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0010756543564 50.9 2 17.031193976335604 23.75 3 6.238795267120832 13.05 4 2.2588741484403014 6.3 5 0.7171029042667623 2.5 6 0.5019720329867335 2.1 7 0.10756543564001435 0.525 8 0.07171029042667623 0.4 9 0.035855145213338116 0.22499999999999998 >10 0.035855145213338116 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTAAACTCAAATCTATTGCTTTGTGAGAGCCTACCATTGCTTGATACCAC 10 0.25 No Hit CAAGAACTACTGGAGGACACACATGAGGAAGAAAGCACAGGAGAGGAAGA 9 0.22499999999999998 No Hit CATATATGTATTGCATTTACTGTTTTCTATAGTTACACAACCACATCCGA 8 0.2 No Hit AGACAGAATTCCTGTGATCGTTGAGAAGGCGGATAAGTCTGATGTTCCAG 8 0.2 No Hit GCTTCCCCTCTCCTCGGGCTTCTTCTTCGGCTCGCACGCACGCGCCCAAC 7 0.17500000000000002 No Hit AGACATTGGCGCCCAACATGGAGACTGGGGAGTCTGACCCAGCAGCTGTT 7 0.17500000000000002 No Hit GGCAATCTCGTCTTCTCCAATCGCCTCGAAGCTCGTCGAATCCTCCCCCA 7 0.17500000000000002 No Hit GCCAAACTGTTGCTGACATGATAAAGGGTAAGACTCCTGAGGAGATCCGC 6 0.15 No Hit TGATGAATCAAAGATGATGGAGAGGCTGTGGGGTGAGAACTTCTTTGACC 6 0.15 No Hit AGATGATTCAGCTGAGCTTGGATGGCAAGAGGGTTTATGTGACCAATTCT 6 0.15 No Hit GTCAAAACTGGGTGTTTGTCTACTGGTAATTTCTCTTTTGGCAGCATCTA 6 0.15 No Hit ACGAGCTTCAATTGTGGGTTTATCAACTCCATCTGGAAGGCTTGCAACTC 6 0.15 No Hit TGGATCTAGAACCAAGGTGCATCAACACGAATCTTGTAGAATCTGGAGTT 6 0.15 No Hit GTATTTTCATATGCTTTGTAGTGTTAGGCTGCTAGCCAATTGGAGGAACA 6 0.15 No Hit AGAAATGGGTTGCAAATCTATGAAATTTCATCATCTGCCTTTGCGGTGTC 6 0.15 No Hit CCGTGAAAGAGAATCGGGGAGCATGTACACAGATTGGGACATCCTACCTC 6 0.15 No Hit CAGAAACCATCCCAAGTACAACTAGTTACTGAAACAACTGATGTAGTTAC 6 0.15 No Hit GCTGTAGCCTGATGTGTAGCCTGTATTCTTAATCACATCATAATATTTAC 6 0.15 No Hit GATGAACAGAAGATTGGTGCTGAAATCGTAAGGAAGTCCTTGAGTTACCC 6 0.15 No Hit AATTAGGATGTGGTATTACAAGGCTCGTTTCTTTTTTAATTTCATCGGAA 6 0.15 No Hit GTGGGATCAGAAGACTGGACGATCAAGAGGTTTCGGTTTTGTTTCTTTCA 6 0.15 No Hit AGCTGCTAAAGTGACTATTACTGATAAGGAGAAGAAGGACGCACTAGACA 5 0.125 No Hit GCAAGGTTGAGGAAATTGATGAAGAGAAGGAAGAAGAAAAGGAAAAGAAG 5 0.125 No Hit GATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGA 5 0.125 No Hit ATTGAACGATCCATTTTGCCCTCTAGCCTACTATGTTGTTCTTCTGTAGT 5 0.125 No Hit CCTTCTTCGGCTTCCTCGGCGCCGCCGCCGCGCTCGTCTTCTCCTGCATG 5 0.125 No Hit GGAGGAGGTAATCGCCAGCCATGGGCGTCACAAAGGAGGACGTGGAGTCC 5 0.125 No Hit CGCTCAAACCCTAGCCGGCCCCGGGGGGAAAGAGGGGAGTCCCGGACGAC 5 0.125 No Hit CGCCACCATGGCTCGCCTGGCAGCCGTCGCCTCCCTCGCCGTCCTCCTTC 5 0.125 No Hit GCCGAGAACAAGGAGGACTACAACAAGTTCTATGAGTCCTTCTCCAAGAA 5 0.125 No Hit ACAACTGGAGATGAGGATGAAGAAGATGGCATTGCGGACCAAAATGTCAC 5 0.125 No Hit TGTTGGTGGTTCCACCAGGATTCCCAGAGTGCAGCAGCTTCTTCAGGACT 5 0.125 No Hit GGTCAGTTGTGTGAGAAGGTGAGAGTATTTGTGAGTCAGTGGGTTCAATT 5 0.125 No Hit TTCCTCTGCATTGCTTGGTCAAAGCAAGGATGGTTCACTTGAATCACTTC 5 0.125 No Hit CTTGAAGAAAACACAGAGAGGAGGTTGGATGTCCCTGGGAGGAATACTGC 5 0.125 No Hit TGTTAGAGGATGAAGAGCAGCAGAGTTTGTACTCCTTTTTGGTGCCGCTA 5 0.125 No Hit CTTAAAAGCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCG 5 0.125 No Hit AAAAGTTTGAAGACCCTGCAGAAGGCGAAGACGTTTTAGTTGCGAAATTC 5 0.125 No Hit AGAAGATGGCATTGAGGGTAATTGCTGAAAGTCTATCTGAGGAGGAGATC 5 0.125 No Hit TAAGAGGGAACCGGGGCAGTACTTCTATTACGGTGCACCAGTTCATGAGC 5 0.125 No Hit ATTTCACCTGAAGTCCCATCACAATATGCAAACACACTTGTATCACCCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.2125 0.0 0.0 0.0 0.0 76-77 0.3375 0.0 0.0 0.0 0.0 78-79 0.4375 0.0 0.0 0.0 0.0 80-81 0.475 0.0 0.0 0.0 0.0 82-83 0.5 0.0 0.0 0.0 0.0 84-85 0.6875 0.0 0.0 0.0 0.0 86-87 0.9875 0.0 0.0 0.0 0.0 88-89 1.2875 0.0 0.0 0.0 0.0 90-91 1.5625 0.0 0.0 0.0 0.0 92-93 2.0125 0.0 0.0 0.0 0.0 94-95 2.2875 0.0 0.0 0.0 0.0 96-97 2.7249999999999996 0.0 0.0 0.0 0.0 98-99 3.225 0.0 0.0 0.0 0.0 100-101 3.55 0.0 0.0 0.0 0.0 102-103 3.875 0.0 0.0 0.0 0.0 104-105 4.487500000000001 0.0 0.0 0.0 0.0 106-107 4.8625 0.0 0.0 0.0 0.0 108-109 5.425 0.0 0.0 0.0 0.0 110-111 5.8375 0.0 0.0 0.0 0.0 112-113 6.574999999999999 0.0 0.0 0.0 0.0 114-115 7.0625 0.0 0.0 0.0 0.0 116-117 7.5375 0.0 0.0 0.0 0.0 118-119 8.3375 0.0 0.0 0.0 0.0 120-121 9.274999999999999 0.0 0.0 0.0 0.0 122-123 10.05 0.0 0.0 0.0 0.0 124-125 10.675 0.0 0.0 0.0 0.0 126-127 11.2375 0.0 0.0 0.0 0.0 128-129 12.1375 0.0 0.0 0.0 0.0 130-131 12.9125 0.0 0.0 0.0 0.0 132-133 13.8625 0.0 0.0 0.0 0.0 134-135 14.675 0.0 0.0 0.0 0.0 136-137 15.8125 0.0 0.0 0.0 0.0 138-139 16.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155599 spots for SRR13165384.sra Written 1155599 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra Read 1155580 spots for SRR13165384.sra Written 1155580 spots for SRR13165384.sra SRR ids: ['SRR13165384.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_2vni7k81 SRR13165384.sra spots: 23111619 blocks: [[1, 1155580], [1155581, 2311160], [2311161, 3466740], [3466741, 4622320], [4622321, 5777900], [5777901, 6933480], [6933481, 8089060], [8089061, 9244640], [9244641, 10400220], [10400221, 11555800], [11555801, 12711380], [12711381, 13866960], [13866961, 15022540], [15022541, 16178120], [16178121, 17333700], [17333701, 18489280], [18489281, 19644860], [19644861, 20800440], [20800441, 21956020], [21956021, 23111619]] SRR13165384 file size 7832638 SRR13165384 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165384 SRR13165384_1.fastq SRR13165384_2.fastq Input file: SRR13165384_1.fastq Paired file: SRR13165384_2.fastq trimmed: SRR13165384-trimmed-pair1.fastq, SRR13165384-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 17:13:07 2024 >> started Sat Dec 7 17:13:32 2024 >> done (24.616s) 23111619 read pairs processed; of these: 654 ( 0.00%) short read pairs filtered out after trimming by size control 69916 ( 0.30%) empty read pairs filtered out after trimming by size control 23041049 (99.69%) read pairs available; of these: 5109538 (22.18%) trimmed read pairs available after processing 17931511 (77.82%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 34 0.00% 19 60 0.00% 20 32 0.00% 21 77 0.00% 22 83 0.00% 23 103 0.00% 24 140 0.00% 25 85 0.00% 26 115 0.00% 27 143 0.00% 28 169 0.00% 29 197 0.00% 30 211 0.00% 31 202 0.00% 32 229 0.00% 33 193 0.00% 34 212 0.00% 35 248 0.00% 36 225 0.00% 37 243 0.00% 38 269 0.00% 39 277 0.00% 40 338 0.00% 41 317 0.00% 42 361 0.00% 43 345 0.00% 44 413 0.00% 45 437 0.00% 46 430 0.00% 47 513 0.00% 48 633 0.00% 49 667 0.00% 50 714 0.00% 51 819 0.00% 52 911 0.00% 53 909 0.00% 54 1037 0.00% 55 1116 0.00% 56 1234 0.01% 57 1347 0.01% 58 1503 0.01% 59 1753 0.01% 60 1928 0.01% 61 2267 0.01% 62 2442 0.01% 63 2788 0.01% 64 3177 0.01% 65 3318 0.01% 66 3765 0.02% 67 4049 0.02% 68 4508 0.02% 69 4959 0.02% 70 5779 0.03% 71 6166 0.03% 72 7118 0.03% 73 8050 0.03% 74 8867 0.04% 75 9535 0.04% 76 10789 0.05% 77 11674 0.05% 78 12620 0.05% 79 13832 0.06% 80 14454 0.06% 81 16391 0.07% 82 17865 0.08% 83 19920 0.09% 84 21766 0.09% 85 24267 0.11% 86 25959 0.11% 87 27324 0.12% 88 28920 0.13% 89 30152 0.13% 90 32237 0.14% 91 33992 0.15% 92 36392 0.16% 93 38092 0.17% 94 41285 0.18% 95 43395 0.19% 96 46228 0.20% 97 48013 0.21% 98 49261 0.21% 99 51415 0.22% 100 52908 0.23% 101 53332 0.23% 102 54861 0.24% 103 57177 0.25% 104 59254 0.26% 105 61484 0.27% 106 64077 0.28% 107 66110 0.29% 108 67618 0.29% 109 69991 0.30% 110 69439 0.30% 111 70969 0.31% 112 72522 0.31% 113 73303 0.32% 114 74913 0.33% 115 76238 0.33% 116 78881 0.34% 117 80074 0.35% 118 81073 0.35% 119 81666 0.35% 120 83150 0.36% 121 84832 0.37% 122 84191 0.37% 123 85490 0.37% 124 86782 0.38% 125 87910 0.38% 126 89202 0.39% 127 90318 0.39% 128 90929 0.39% 129 92754 0.40% 130 92554 0.40% 131 93339 0.41% 132 94525 0.41% 133 94476 0.41% 134 94453 0.41% 135 95879 0.42% 136 96148 0.42% 137 96595 0.42% 138 98730 0.43% 139 99394 0.43% 140 100093 0.43% 141 100183 0.43% 142 100251 0.44% 143 99941 0.43% 144 101231 0.44% 145 102282 0.44% 146 100565 0.44% 147 102627 0.45% 148 102912 0.45% 149 104051 0.45% 150 103558 0.45% 151 17931511 77.82% 23041049 reads passed initial QC criterion=sequence-density sequence-density=0.15 sequence-density-rank=1 fanout-score=18.39 fanout-score-rank=9 prefix-density=0.32 prefix-fanout=8.8 sequence=TCTCCAGCTCCTT criterion=fanout-score sequence-density=0.10 sequence-density-rank=16 fanout-score=229.28 fanout-score-rank=1 prefix-density=0.92 prefix-fanout=23.6 sequence=CTTCTTCTTCCT criterion=sequence-density sequence-density=0.25 sequence-density-rank=1 fanout-score=29.18 fanout-score-rank=7 prefix-density=0.61 prefix-fanout=12.0 sequence=AGGAAGAAGAAG criterion=fanout-score sequence-density=0.05 sequence-density-rank=33 fanout-score=241.84 fanout-score-rank=1 prefix-density=1.12 prefix-fanout=11.5 sequence=AAGAAGAAGGTGGAGTCCAAGAACGCCCTGGAGAACTACTCGTACAACATGCGCAACACCATCAAGGACGAGAAGATCGCCTCCAAGCTGCCGGCGGACGACAAGAAGAAGATCGAGGACGCCATTGATGCTGCCATCCAGTG SRR13165384 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:14:22 Started mapping on | Dec 07 17:14:23 Finished on | Dec 07 17:16:19 Mapping speed, Million of reads per hour | 715.07 Number of input reads | 23041049 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 22058851 Uniquely mapped reads % | 95.74% Average mapped length | 287.75 Number of splices: Total | 20368888 Number of splices: Annotated (sjdb) | 18895019 Number of splices: GT/AG | 20052429 Number of splices: GC/AG | 270419 Number of splices: AT/AC | 12468 Number of splices: Non-canonical | 33572 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.46 Insertion rate per base | 0.01% Insertion average length | 2.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 251837 % of reads mapped to multiple loci | 1.09% Number of reads mapped to too many loci | 43826 % of reads mapped to too many loci | 0.19% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.13% % of reads unmapped: other | 0.85% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 730616 730616 730616 N_multimapping 251837 251837 251837 N_noFeature 964486 21368306 1207268 N_ambiguous 524920 2749 77784 UnstrandedReadsAssigned:20569445 PositiveStrandReadsAssigned:687796 NegativeStrandReadsAssigned:20773799 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=146 echo kmer=141 SRR13165384 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165384-trimmed-pair1.fastq SRR13165384-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,041,049 reads, 21,014,233 reads pseudoaligned [quant] estimated average fragment length: 227.04 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,174 rounds 52973 SRR13165384.ke.tsv 35125 SRR13165384.se.tsv 88098 total ==> SRR13165384.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 710.502 0 0 PNS24247 1044 817.96 95.5592 8.31167 PNS24249 1928 1701.96 203.241 8.49591 PNS24246 1044 817.96 95.5592 8.31167 PNS24248 1044 817.96 95.5592 8.31167 PNS24244 1471 1244.96 233.081 13.3199 PNS24243 293 113.835 0 0 KQK14069 1603 1376.96 32099.9 1658.56 KQK14071 474 263.451 605.425 163.497 ==> SRR13165384.se.tsv <== BRADI_1g14170v3 34928 BRADI_1g53295v3 443 BRADI_1g59795v3 1141 BRADI_1g07683v3 0 BRADI_1g00485v3 28 BRADI_1g20270v3 565 BRADI_1g74790v3 663 BRADI_1g09890v3 0 BRADI_1g77505v3 364 BRADI_1g48960v3 0 SRR13165384 completed mapping pipeline successfully