Starting /dee2/code/volunteer_pipeline.sh SRR13165385 current disk space = 1541434626048 free memory = 1430254748 SRR13165385 SRAfilesize ee7a3559f3ebd147a3b3f4f9e896a8ad SRR13165385.sra SRR13165385.sra file validated SRR13165385 is paired end SRR13165385 is conventional basespace SRR13165385 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165385_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.589 37.0 37.0 37.0 37.0 37.0 2 36.203 37.0 37.0 37.0 37.0 37.0 3 36.56 37.0 37.0 37.0 37.0 37.0 4 36.5335 37.0 37.0 37.0 37.0 37.0 5 36.532 37.0 37.0 37.0 37.0 37.0 6 36.554 37.0 37.0 37.0 37.0 37.0 7 36.4795 37.0 37.0 37.0 37.0 37.0 8 36.611 37.0 37.0 37.0 37.0 37.0 9 36.548 37.0 37.0 37.0 37.0 37.0 10-14 36.5517 37.0 37.0 37.0 37.0 37.0 15-19 36.5053 37.0 37.0 37.0 37.0 37.0 20-24 36.5227 37.0 37.0 37.0 37.0 37.0 25-29 36.5031 37.0 37.0 37.0 37.0 37.0 30-34 36.4668 37.0 37.0 37.0 37.0 37.0 35-39 36.3971 37.0 37.0 37.0 37.0 37.0 40-44 36.395 37.0 37.0 37.0 37.0 37.0 45-49 36.2313 37.0 37.0 37.0 37.0 37.0 50-54 36.380799999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.2866 37.0 37.0 37.0 37.0 37.0 60-64 36.2093 37.0 37.0 37.0 37.0 37.0 65-69 36.0427 37.0 37.0 37.0 37.0 37.0 70-74 36.2162 37.0 37.0 37.0 37.0 37.0 75-79 36.2807 37.0 37.0 37.0 37.0 37.0 80-84 36.2377 37.0 37.0 37.0 37.0 37.0 85-89 36.2904 37.0 37.0 37.0 37.0 37.0 90-94 36.2419 37.0 37.0 37.0 37.0 37.0 95-99 36.2509 37.0 37.0 37.0 37.0 37.0 100-104 36.2257 37.0 37.0 37.0 37.0 37.0 105-109 36.1659 37.0 37.0 37.0 37.0 37.0 110-114 36.190099999999994 37.0 37.0 37.0 37.0 37.0 115-119 36.141200000000005 37.0 37.0 37.0 37.0 37.0 120-124 36.0453 37.0 37.0 37.0 37.0 37.0 125-129 36.0329 37.0 37.0 37.0 37.0 37.0 130-134 36.034000000000006 37.0 37.0 37.0 37.0 37.0 135-139 35.9348 37.0 37.0 37.0 37.0 37.0 140-144 35.854099999999995 37.0 37.0 37.0 37.0 37.0 145-149 35.58239999999999 37.0 37.0 37.0 37.0 37.0 150-151 35.396 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 1.0 24 2.0 25 6.0 26 1.0 27 12.0 28 13.0 29 19.0 30 29.0 31 25.0 32 46.0 33 76.0 34 157.0 35 347.0 36 2838.0 37 427.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 50.025 10.15 6.15 33.675 2 24.39516129032258 12.550403225806454 29.98991935483871 33.064516129032256 3 21.275 15.075 24.575 39.074999999999996 4 26.125 20.45 21.099999999999998 32.324999999999996 5 28.575 26.625 21.675 23.125 6 26.25 29.799999999999997 21.75 22.2 7 19.900000000000002 26.6 34.599999999999994 18.9 8 18.925 24.8 29.475 26.8 9 21.099999999999998 20.474999999999998 32.175 26.25 10-14 23.22 26.365 24.635 25.779999999999998 15-19 23.735 25.965 24.945 25.355 20-24 23.515 26.505000000000003 24.675 25.305 25-29 23.595 25.965 24.345 26.095000000000002 30-34 22.59 24.779999999999998 25.740000000000002 26.889999999999997 35-39 23.74 24.610000000000003 25.580000000000002 26.07 40-44 23.615 24.759999999999998 25.080000000000002 26.545 45-49 23.205000000000002 25.35 25.195 26.25 50-54 23.425 24.215 25.485000000000003 26.875 55-59 23.380000000000003 25.259999999999998 24.645 26.715 60-64 23.87 24.355 25.759999999999998 26.015 65-69 24.23 25.775 24.705 25.290000000000003 70-74 25.3 24.315 24.18 26.205000000000002 75-79 25.91 25.074999999999996 23.365 25.650000000000002 80-84 25.81 24.37 24.55 25.27 85-89 25.085 25.290000000000003 24.04 25.585 90-94 25.685000000000002 24.615000000000002 23.97 25.729999999999997 95-99 25.740000000000002 25.035 24.305 24.92 100-104 25.435000000000002 24.94 23.84 25.785000000000004 105-109 25.25 23.544999999999998 24.610000000000003 26.595000000000002 110-114 26.005 24.575 24.315 25.105 115-119 26.200000000000003 24.765 23.41 25.624999999999996 120-124 25.369999999999997 24.795 23.18 26.655 125-129 25.495 24.34 23.935000000000002 26.229999999999997 130-134 25.245 24.529999999999998 24.03 26.195 135-139 25.674999999999997 23.880000000000003 24.560000000000002 25.885 140-144 25.6 24.705 23.7 25.995 145-149 25.979999999999997 24.47 24.104999999999997 25.445 150-151 25.025 23.799999999999997 24.0125 27.1625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 1.0 27 1.0 28 0.0 29 0.5 30 2.5 31 5.0 32 12.0 33 14.5 34 20.5 35 30.5 36 34.5 37 48.0 38 74.5 39 85.0 40 106.0 41 139.5 42 132.5 43 157.0 44 189.0 45 198.0 46 214.0 47 199.0 48 190.0 49 193.0 50 165.5 51 158.0 52 163.0 53 144.5 54 116.5 55 97.0 56 91.5 57 79.0 58 74.0 59 73.5 60 71.0 61 71.0 62 64.5 63 60.0 64 64.0 65 68.0 66 69.0 67 53.5 68 42.0 69 39.5 70 26.0 71 25.5 72 25.0 73 21.0 74 23.0 75 16.5 76 11.5 77 9.5 78 7.0 79 4.0 80 3.5 81 3.5 82 2.0 83 1.0 84 1.5 85 3.5 86 2.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.8 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.225 #Duplication Level Percentage of deduplicated Percentage of total 1 70.47229453328376 47.375 2 19.04053551506136 25.6 3 6.396429899590926 12.9 4 2.268501301599107 6.1 5 1.1528449237634808 3.875 6 0.334696913350688 1.35 7 0.2231312755671253 1.05 8 0.0 0.0 9 0.0 0.0 >10 0.11156563778356265 1.7500000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCTTATCTCGTAT 44 1.0999999999999999 TruSeq Adapter, Index 2 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCTTATCGCGTAT 16 0.4 TruSeq Adapter, Index 2 (97% over 38bp) CACGTGGTATCTCAAGTAAGCTTGTGATACGGAAATACTAGTTTGCAAGG 10 0.25 No Hit GTGAGCACTTTATTGGCTTGCCACGGATGACCAATCCATTGGCCATCTGA 7 0.17500000000000002 No Hit CCCTGCAGAAATAGCTACCTTTATAGAAAGCATATATTTTGCTTTGTTTT 7 0.17500000000000002 No Hit GCTGAATATACAAGGATGGTGGACTTATGGACAGTTTTGTTGAGGTAACT 7 0.17500000000000002 No Hit CCCTGTGAAACGAATGAGGCCTAAATGACACCCTCGAAGATATGCATCAA 7 0.17500000000000002 No Hit ATTGCGTCTTGCCGAACTTGGGTCGACCAGCCTTTTGATGGACGAAGGCT 7 0.17500000000000002 No Hit CACCAGTTACGCCACCACTAGCCTATACTACTTACAAGTACAACATCGTG 7 0.17500000000000002 No Hit GTGGCGCTGGTGATGTTGTAGAGGCGTATGGAGTCGTCCTCGCTCGAGGT 6 0.15 No Hit CCCCACCACACTGGAGATGTTGCCGTTAGCCTTCAAGCACCGTCCCCTAT 6 0.15 No Hit CCTTGCAGATAAACTCGGCACTAAACTGATTTAGGCCTTGGCAGCAGCCT 6 0.15 No Hit CAACAAGTTCCTTCAAAAGAAAAAGAAAGGGAAGGATGGCAAGGGCAAGA 6 0.15 No Hit GGTCACTCAGCGACATCTGACACTCCTCGTCCGTGAACACACGCCTGGGG 6 0.15 No Hit CACGCAGGCCGGCATCACCTGGCCATCTTCGGTCACCGAAGCGTTGAGAT 6 0.15 No Hit GCAGGATCCCGCCCATAAAGTGACACACCTGAGCGATGAATGTTATGATC 6 0.15 No Hit CTCCGCATTCCATTACACGGCCAACTTCCTCGCCACTAGCATCAAAACGA 6 0.15 No Hit GTCGAACTTGCCGCTGTCCACGGCGCTGCTGTCGGGGTTCTCCGAGAAGA 6 0.15 No Hit CACGACAGTGCCCTCTGGAAGGGAAGACAAGTGCACAAGAGGAGTATGTG 5 0.125 No Hit GCTTCAACAAAAGGAAAGTAACTTCTTGTGCTTCCAGTCACCGAAATACC 5 0.125 No Hit GCCCTGGCTGGCATCACGAAGTAGTGGCAGATCAATCCGCCGGCCAGTGC 5 0.125 No Hit GCTTAATTTATTGGCCCATAGGATATATCAGGCCCAACATATATCCCTTG 5 0.125 No Hit GCCTTCTCTAGCCAAGTACGGAGGCACAAGCTAGCTCTACGACTACGTGT 5 0.125 No Hit GGTCAATGTCCGGCAGCTGAAGCCCACGCAAGAGGTCCTGGTAGAACTCT 5 0.125 No Hit CCCATAAGCTACACAGAGCCAGCAAATCCAAATAGGCTACAGGTTTCATT 5 0.125 No Hit GGCCATCCTTGGAGATACCAGCCTCAAAACCTCCAGTCGTGGAGTCAATG 5 0.125 No Hit GCGCCTTGCTATGACAATGCCTTTCAATGTAGACTCACGTCGCTTGTTCT 5 0.125 No Hit CCAGCCTGCTGCTCAAGTCTAGGCCGCTTTGGTCTCTCGAAGCCTGGAGG 5 0.125 No Hit GCTTCTCTCTTATTTCCATCCACACTTCTTCACTATAGTCTGCACTTCGA 5 0.125 No Hit CTGTCACTTGGCGCAGAGCAGCAGCAGTAAGCTGGTGGTGGTGGTAACTT 5 0.125 No Hit GGGGGCGTGTATATTACATGGATGGAATTTTCCTCTACATACAGCAGGGC 5 0.125 No Hit GCCCAATCTGGCAGTTTTATCTTCCCATTTTCGATGGGCACCTTCTCAAC 5 0.125 No Hit CGACGGAGTTGCAAGAGAAAAATCGCGGCATCCTGTTTTGTACAAAATCA 5 0.125 No Hit AGGTTTGCCTGTTTTCTCTTCCTCCAGTACGATCACGATGAATAGGAAGT 5 0.125 No Hit TTTTTTTTTAAGAACAGATGGTTGGAACCATTACAGAGGCATTTTGTGGA 5 0.125 No Hit ACCAGAGTAGACTCACTGACTAATTCTTTTTGAACATTTGAATCCTCACC 5 0.125 No Hit ACAGCACCAGCGCCAGTAGCATGCTTCTGCAGGGCAGCAGCAGGGCTCAC 5 0.125 No Hit CACATGATGATAATTTCTATTTCTTGAAGCCAAGATGCAGATTTGCCCGT 5 0.125 No Hit ATCTTCTCAACAGTCAACACAATGCTAAATTGCCGTGCGACAGTAAACTT 5 0.125 No Hit CCAGGCGATTGTCAAAGTCCTCCCCACCAAGGTGAGTGTCACCAGCTGTT 5 0.125 No Hit CTCGCGGTTTCGAGCAAGTACCGATCTTTTAGCAGCAATTGCATTAGCAG 5 0.125 No Hit CCTTGGGACCGCATGTTCTTGCCTGGTTCGTCGGGGACCGTCCGTTTGTT 5 0.125 No Hit GCACAGGGAAGCACATTGACAGCGATGGTGATGAGCATGGGCACATCAAC 5 0.125 No Hit GGAGGAGGCATGAACATGGTGGGAGTTGGTAAGCAAGCATCAGTCTGGTC 5 0.125 No Hit CACGGACAACCATATCACGTTCCCAGGTGCCACCACCCATGTCAAGAATA 5 0.125 No Hit GTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGT 5 0.125 No Hit ATGACTTTGAATGCAAAATATATAAATCTTTGATGTTACTCTCAAACTTC 5 0.125 No Hit GCCGTCGCCGCCGCCGTAGCGCCCACCACCACCGCCTCCGCCGTAGCGGT 5 0.125 No Hit CCTGAATATCTGTTGTCAAATTACAAATTTTGGGAAGAAACATAGGTTAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.1 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.15 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.225 0.0 0.0 0.0 0.0 68-69 0.2375 0.0 0.0 0.0 0.0 70-71 0.375 0.0 0.0 0.0 0.0 72-73 0.44999999999999996 0.0 0.0 0.0 0.0 74-75 0.5125 0.0 0.0 0.0 0.0 76-77 0.6625000000000001 0.0 0.0 0.0 0.0 78-79 0.75 0.0 0.0 0.0 0.0 80-81 0.8375 0.0 0.0 0.0 0.0 82-83 1.0125 0.0 0.0 0.0 0.0 84-85 1.0875 0.0 0.0 0.0 0.0 86-87 1.3125 0.0 0.0 0.0 0.0 88-89 1.7125 0.0 0.0 0.0 0.0 90-91 2.2375 0.0 0.0 0.0 0.0 92-93 2.8375 0.0 0.0 0.0 0.0 94-95 3.45 0.0 0.0 0.0 0.0 96-97 3.7875 0.0 0.0 0.0 0.0 98-99 4.15 0.0 0.0 0.0 0.0 100-101 4.7125 0.0 0.0 0.0 0.0 102-103 5.1875 0.0 0.0 0.0 0.0 104-105 5.7875 0.0 0.0 0.0 0.0 106-107 6.525 0.0 0.0 0.0 0.0 108-109 7.275 0.0 0.0 0.0 0.0 110-111 8.0125 0.0 0.0 0.0 0.0 112-113 8.6625 0.0 0.0 0.0 0.0 114-115 9.4125 0.0 0.0 0.0 0.0 116-117 10.25 0.0 0.0 0.0 0.0 118-119 11.1375 0.0 0.0 0.0 0.0 120-121 11.837499999999999 0.0 0.0 0.0 0.0 122-123 12.4875 0.0 0.0 0.0 0.0 124-125 13.2 0.0 0.0 0.0 0.0 126-127 13.787500000000001 0.0 0.0 0.0 0.0 128-129 14.412500000000001 0.0 0.0 0.0 0.0 130-131 15.275 0.0 0.0 0.0 0.0 132-133 16.075 0.0 0.0 0.0 0.0 134-135 16.5625 0.0 0.0 0.0 0.0 136-137 17.1875 0.0 0.0 0.0 0.0 138-139 18.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCACAT 10 0.006830828 145.0 8 CACCACA 10 0.006830828 145.0 7 >>END_MODULE SRR13165385 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165385_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0905 37.0 37.0 37.0 37.0 37.0 2 36.2385 37.0 37.0 37.0 37.0 37.0 3 36.203 37.0 37.0 37.0 37.0 37.0 4 36.21 37.0 37.0 37.0 37.0 37.0 5 36.239 37.0 37.0 37.0 37.0 37.0 6 36.2545 37.0 37.0 37.0 37.0 37.0 7 36.2615 37.0 37.0 37.0 37.0 37.0 8 36.218 37.0 37.0 37.0 37.0 37.0 9 36.117 37.0 37.0 37.0 37.0 37.0 10-14 36.084799999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.1323 37.0 37.0 37.0 37.0 37.0 20-24 36.07190000000001 37.0 37.0 37.0 37.0 37.0 25-29 35.88719999999999 37.0 37.0 37.0 37.0 37.0 30-34 35.8061 37.0 37.0 37.0 37.0 37.0 35-39 35.8323 37.0 37.0 37.0 37.0 37.0 40-44 35.805600000000005 37.0 37.0 37.0 37.0 37.0 45-49 35.7618 37.0 37.0 37.0 37.0 37.0 50-54 35.718 37.0 37.0 37.0 37.0 37.0 55-59 35.730500000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.7553 37.0 37.0 37.0 37.0 37.0 65-69 35.689350000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.591899999999995 37.0 37.0 37.0 37.0 37.0 75-79 35.5944 37.0 37.0 37.0 37.0 37.0 80-84 35.60549999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.75169999999999 37.0 37.0 37.0 37.0 37.0 90-94 35.7602 37.0 37.0 37.0 37.0 37.0 95-99 35.7479 37.0 37.0 37.0 37.0 37.0 100-104 35.79299999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.682300000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.5575 37.0 37.0 37.0 37.0 37.0 115-119 35.5092 37.0 37.0 37.0 37.0 37.0 120-124 35.438199999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.3299 37.0 37.0 37.0 37.0 37.0 130-134 35.017399999999995 37.0 37.0 37.0 27.4 37.0 135-139 34.8511 37.0 37.0 37.0 27.4 37.0 140-144 34.5399 37.0 37.0 37.0 25.0 37.0 145-149 34.399499999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.086 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 7.0 14 5.0 15 5.0 16 2.0 17 4.0 18 4.0 19 5.0 20 5.0 21 1.0 22 9.0 23 11.0 24 10.0 25 5.0 26 10.0 27 24.0 28 30.0 29 22.0 30 33.0 31 55.0 32 76.0 33 127.0 34 220.0 35 468.0 36 2550.0 37 308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.10552763819096 20.326633165829143 8.743718592964825 24.824120603015075 2 32.9 19.85 25.224999999999998 22.025 3 24.75 24.525 28.325 22.400000000000002 4 27.875 30.875000000000004 20.45 20.8 5 30.925000000000004 32.175 17.625 19.275000000000002 6 26.575 33.324999999999996 19.275000000000002 20.825 7 25.5 19.275000000000002 32.425 22.8 8 27.35 21.95 22.325 28.375 9 24.025 22.725 26.200000000000003 27.05 10-14 28.64 25.795 21.305 24.26 15-19 27.235 25.490000000000002 22.655 24.62 20-24 27.938969484742373 24.73736868434217 22.876438219109556 24.447223611805903 25-29 27.838919459729865 24.482241120560282 23.956978489244623 23.721860930465233 30-34 27.478739369684842 24.117058529264632 23.641820910455227 24.7623811905953 35-39 27.878939469734863 24.617308654327164 23.16658329164582 24.337168584292147 40-44 27.068534267133565 24.972486243121562 23.08654327163582 24.872436218109055 45-49 27.063531765882942 25.162581290645324 23.936968484242122 23.836918459229615 50-54 27.39869934967484 24.862431215607803 24.12206103051526 23.6168084042021 55-59 28.23411705852926 25.27263631815908 22.93146573286643 23.56178089044522 60-64 27.823911955977987 23.466733366683343 24.757378689344673 23.951975987993997 65-69 27.702466109749384 24.20589265169326 24.270921914861688 23.820719323695663 70-74 28.049024512256125 24.072036018009005 24.297148574287146 23.581790895447725 75-79 27.848924462231118 24.58729364682341 23.76688344172086 23.796898449224614 80-84 27.5887943971986 24.667333666833414 23.856928464232116 23.88694347173587 85-89 27.358679339669834 24.76738369184592 23.896948474237117 23.976988494247124 90-94 27.748874437218607 25.552776388194097 23.36168084042021 23.336668334167083 95-99 27.678839419709856 25.432716358179093 23.556778389194598 23.33166583291646 100-104 29.264632316158078 24.937468734367183 22.58129064532266 23.216608304152075 105-109 29.184592296148075 24.252126063031515 23.451725862931465 23.111555777888945 110-114 29.079539769884942 25.032516258129068 23.376688344172088 22.511255627813906 115-119 29.959979989994995 25.58279139569785 21.9959979989995 22.461230615307652 120-124 29.84992496248124 24.87743871935968 22.811405702851424 22.461230615307652 125-129 29.969984992496247 24.842421210605302 23.03151575787894 22.156078039019512 130-134 30.050025012506254 25.18759379689845 23.16658329164582 21.595797898949478 135-139 30.86543271635818 24.402201100550275 22.88144072036018 21.850925462731364 140-144 32.46123061530765 24.41720860430215 23.19159579789895 19.929964982491246 145-149 33.5767883941971 23.991995997999 22.59629814907454 19.834917458729365 150-151 33.96698349174587 23.19909954977489 22.648824412206103 20.185092546273136 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.5 7 1.0 8 1.0 9 1.5 10 1.5 11 0.5 12 0.5 13 1.0 14 1.5 15 1.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 0.5 26 0.5 27 0.5 28 1.5 29 1.5 30 1.5 31 6.0 32 10.0 33 16.0 34 23.0 35 21.0 36 33.0 37 57.0 38 69.0 39 77.5 40 104.5 41 127.5 42 137.5 43 153.0 44 159.0 45 159.5 46 173.0 47 197.5 48 200.5 49 184.0 50 172.5 51 155.5 52 128.0 53 119.5 54 120.0 55 124.0 56 107.5 57 85.5 58 82.0 59 74.0 60 69.0 61 67.5 62 66.0 63 56.5 64 54.5 65 61.0 66 53.5 67 55.0 68 54.0 69 50.5 70 44.0 71 33.5 72 37.5 73 30.5 74 28.0 75 21.5 76 10.0 77 10.0 78 8.5 79 4.0 80 2.5 81 3.0 82 3.5 83 3.5 84 2.0 85 0.5 86 1.5 87 2.5 88 1.5 89 1.5 90 2.5 91 2.5 92 4.0 93 5.5 94 4.5 95 3.0 96 5.0 97 6.5 98 4.0 99 6.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.05 30-34 0.05 35-39 0.05 40-44 0.05 45-49 0.05 50-54 0.05 55-59 0.05 60-64 0.05 65-69 0.045 70-74 0.05 75-79 0.05 80-84 0.05 85-89 0.05 90-94 0.05 95-99 0.05 100-104 0.05 105-109 0.05 110-114 0.05 115-119 0.05 120-124 0.05 125-129 0.05 130-134 0.05 135-139 0.05 140-144 0.05 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.5 #Duplication Level Percentage of deduplicated Percentage of total 1 71.45985401459855 48.949999999999996 2 18.43065693430657 25.25 3 6.167883211678832 12.675 4 2.226277372262774 6.1 5 1.0583941605839415 3.6249999999999996 6 0.36496350364963503 1.5 7 0.21897810218978103 1.05 8 0.0 0.0 9 0.0 0.0 >10 0.072992700729927 0.8500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 24 0.6 No Hit TGACCTCTCGTCGCAGGTTCAGCATGTGGGTGCTCTTGAGAGCATCAGCG 10 0.25 No Hit TTCAAGGGTATTCCAGTTTAATGTGCAAAATTTTCACTGTGTAATTAGCG 7 0.17500000000000002 No Hit GAAGGATTGGAGATCGAAATTCTATTGCTTAATTCTTCTGAAGAGGGTGG 7 0.17500000000000002 No Hit GGAATCAATGAACTGGCCACACATAGTGAGCTCCTTCATGTTCATTTGAA 7 0.17500000000000002 No Hit CATGGACAGAGTACCGGTAAATCCTCCCTAAACCGCAAACCTGTCTAATG 7 0.17500000000000002 No Hit CGAAAAGCACGGCCATGGCGACCAGTGTCTACCTCCCCTCGTCATTCGCC 7 0.17500000000000002 No Hit CGCCACTTCTATAACTTAGGTGTTGGGGCAATCGAGGAAGTTCGTGTTCA 7 0.17500000000000002 No Hit GGTTGAATTCAGTGGATATTAGTAAGCAGGAGCCAGTTGCTCTTGCCCTT 6 0.15 No Hit CTTTCTAGAAGGGAATATGATGGGAAGGTCGCTGATGTTTGGTCCTGTGG 6 0.15 No Hit AGAAATTCTGAGCCAGCAGAAGCAGTGCAAAGGACTGACAGTCCTCCTTC 6 0.15 No Hit CGCTAACCGAGAACAAGAACGCTGACTGCCTTGCTGAACAGCTAGCAAAG 6 0.15 No Hit CCCTCATCAAGGCCAACGACACCATCAAGATCGACCTGGAGACCAACAAG 6 0.15 No Hit CTTGAAGCTGAGGGTTCAATTTACTATCTGTCGGCTGACCATCGTTTTGA 6 0.15 No Hit ACTGGATAACGCAGTTTGCTGCAAGGATAACCGTTCTTGTTGCCCCCATG 6 0.15 No Hit CGGCGGCGGCGCGGTCGAGCGGGAGATGGCGACGTCGCTGTCGCAGCTGG 6 0.15 No Hit GCCGATCCAAGCCTCGCTGGTGAACTCGGCAAGCAAGAACACCGAGAATT 6 0.15 No Hit GTGAAACAGAGAAAGTTCTAAAAGCACTGCAAGCAGTTTCTAATCATCTC 6 0.15 No Hit AGAGGGTGTGGCTTGGCACCTTCGACACTGCTGCGGCGGCCTCCGTCGCG 5 0.125 No Hit GTTCGGCTTCATCACCCCGGAAGATGGCGGCGACGACCTCTTCGTCCACC 5 0.125 No Hit GCATCCTCGTTAACAAATGCAGACATTTCATGTGGCCTTAAAAGCCACCA 5 0.125 No Hit GTAATGTCCTGGCAAGTAAGGACAAACAATTGACACACTTCTTCATAGCT 5 0.125 No Hit TTCCCAATTAGCATCTAAGCTCTCGTTGACATTTCCTTGAGAAGAAGAAG 5 0.125 No Hit GTCCATTTCAGTCTCTGCAAGAGGGACAATACCAAGCAGTTCCACAACTC 5 0.125 No Hit GTCCGGATCATACCACGAAGTCCACCGATCCGACAGTTCTGCAGGATCAC 5 0.125 No Hit GTCGTGGGCGATCGGCAAGCGGAGGAGAGCGGAGGGCGGGTTCCTGGGCG 5 0.125 No Hit GCCATGAGGATGATTCGATCATTGAGCTTGCAAATGAGGTGAGGAAGGTC 5 0.125 No Hit AGTCGACTTGTGAAAACGATCTGATTCTAGAAGTACAGCATGAGCTAATG 5 0.125 No Hit GGCAGGACCTGCAGCCCAGCCTCGGCGCCGACAAGGCGGCTCAGGCTGTT 5 0.125 No Hit CTCAGTTTAAGATTGTCAGCTTCCTCTAGTGCGGATGAAGGTCTTAAGGC 5 0.125 No Hit GGCGGCGGCGGCGTCAAGCTGCCCAAGATCACGCTGGTGTTCGACGGGCC 5 0.125 No Hit GAACAAGAGGTCATTCAAGTACGCGTGGGTGCTTGACAAGCTGAAGGCTG 5 0.125 No Hit GCCAAAGGCAGCCTTATAAGAGGGAAACCTGTGTGTTCGTGGAAGGCAAC 5 0.125 No Hit GTCTAGGTGAATACAATGAGAGAGAGGTTCTGCGCATCATGCCTACGTGC 5 0.125 No Hit ACAGGATCAATACCTAACCCGGAGAAAACAGATTTATGTGGTGAAGATTC 5 0.125 No Hit GGAACTTCTTCGCACCCAGTTGCAAGTTGCTCGAACAGAGGTTGAACATG 5 0.125 No Hit CCAGAGGCAGGCAACTAAGGATGCTGGTGTTATTGCTGGTCTTAATGTCA 5 0.125 No Hit ATGCAGACAATCTGTGAATGCCTAAGGGACACTGTTTGCAAGTTGACTGA 5 0.125 No Hit AGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAATCTCGTG 5 0.125 No Hit AGGAGAAGTCACGATGAAGCTGTTCGTGAAGACCCTCAAGGGCACGAACT 5 0.125 No Hit CAAACTGAATGTGAGAGAGGAGGGGAGGGTGTATGTTTTGAATATGTACT 5 0.125 No Hit GAAAATTCCAGCTCTCCCATGGCTGAGCACCCGCCACGTATCAAGTTCAA 5 0.125 No Hit AGGAACAACAGCTCGTCGAGCTGAGCCTCGAACCCCATGTCAGCCCCAAT 5 0.125 No Hit ATTTTGTTGTTTATTCGTTGAATCAGGCTTGTGAGACTACTGTGTGTACA 5 0.125 No Hit AAGATCTTCAAGTTCATCACGGCCAAGCTCTTCAACCTGCCCTGGACGGT 5 0.125 No Hit TTTCTATCGACGCTACAGCAGTACCACCATTGCAATTTGTCTGATATATC 5 0.125 No Hit ACCTATGCTGCGAAGGTAGCCCAAACAGCAGCAACCGAGAAACCTGCGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.1 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.15 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.225 0.0 0.0 0.0 0.0 68-69 0.2375 0.0 0.0 0.0 0.0 70-71 0.375 0.0 0.0 0.0 0.0 72-73 0.42500000000000004 0.0 0.0 0.0 0.0 74-75 0.4875 0.0 0.0 0.0 0.0 76-77 0.6375 0.0 0.0 0.0 0.0 78-79 0.725 0.0 0.0 0.0 0.0 80-81 0.8125 0.0 0.0 0.0 0.0 82-83 0.9874999999999999 0.0 0.0 0.0 0.0 84-85 1.0625 0.0 0.0 0.0 0.0 86-87 1.2375 0.0 0.0 0.0 0.0 88-89 1.6375 0.0 0.0 0.0 0.0 90-91 2.1624999999999996 0.0 0.0 0.0 0.0 92-93 2.7625 0.0 0.0 0.0 0.0 94-95 3.375 0.0 0.0 0.0 0.0 96-97 3.7125 0.0 0.0 0.0 0.0 98-99 4.1 0.0 0.0 0.0 0.0 100-101 4.6625 0.0 0.0 0.0 0.0 102-103 5.1625 0.0 0.0 0.0 0.0 104-105 5.7625 0.0 0.0 0.0 0.0 106-107 6.4875 0.0 0.0 0.0 0.0 108-109 7.1875 0.0 0.0 0.0 0.0 110-111 7.9 0.0 0.0 0.0 0.0 112-113 8.5375 0.0 0.0 0.0 0.0 114-115 9.2875 0.0 0.0 0.0 0.0 116-117 10.1375 0.0 0.0 0.0 0.0 118-119 11.0625 0.0 0.0 0.0 0.0 120-121 11.7625 0.0 0.0 0.0 0.0 122-123 12.4125 0.0 0.0 0.0 0.0 124-125 13.125 0.0 0.0 0.0 0.0 126-127 13.712499999999999 0.0 0.0 0.0 0.0 128-129 14.3625 0.0 0.0 0.0 0.0 130-131 15.175 0.0 0.0 0.0 0.0 132-133 15.95 0.0 0.0 0.0 0.0 134-135 16.45 0.0 0.0 0.0 0.0 136-137 17.0625 0.0 0.0 0.0 0.0 138-139 17.9125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064498 spots for SRR13165385.sra Written 2064498 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra Read 2064483 spots for SRR13165385.sra Written 2064483 spots for SRR13165385.sra SRR ids: ['SRR13165385.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_loa24vd7 SRR13165385.sra spots: 41289675 blocks: [[1, 2064483], [2064484, 4128966], [4128967, 6193449], [6193450, 8257932], [8257933, 10322415], [10322416, 12386898], [12386899, 14451381], [14451382, 16515864], [16515865, 18580347], [18580348, 20644830], [20644831, 22709313], [22709314, 24773796], [24773797, 26838279], [26838280, 28902762], [28902763, 30967245], [30967246, 33031728], [33031729, 35096211], [35096212, 37160694], [37160695, 39225177], [39225178, 41289675]] SRR13165385 file size 14010337 SRR13165385 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165385 SRR13165385_1.fastq SRR13165385_2.fastq Input file: SRR13165385_1.fastq Paired file: SRR13165385_2.fastq trimmed: SRR13165385-trimmed-pair1.fastq, SRR13165385-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 17:20:15 2024 >> started Sat Dec 7 17:25:40 2024 >> done (324.479s) 41289675 read pairs processed; of these: 1152 ( 0.00%) short read pairs filtered out after trimming by size control 548566 ( 1.33%) empty read pairs filtered out after trimming by size control 40739957 (98.67%) read pairs available; of these: 9951991 (24.43%) trimmed read pairs available after processing 30787966 (75.57%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 56 0.00% 19 63 0.00% 20 74 0.00% 21 91 0.00% 22 113 0.00% 23 170 0.00% 24 257 0.00% 25 247 0.00% 26 259 0.00% 27 263 0.00% 28 363 0.00% 29 324 0.00% 30 471 0.00% 31 408 0.00% 32 515 0.00% 33 434 0.00% 34 494 0.00% 35 501 0.00% 36 511 0.00% 37 575 0.00% 38 628 0.00% 39 749 0.00% 40 832 0.00% 41 875 0.00% 42 865 0.00% 43 947 0.00% 44 883 0.00% 45 1074 0.00% 46 1151 0.00% 47 1278 0.00% 48 1516 0.00% 49 1764 0.00% 50 1855 0.00% 51 2226 0.01% 52 2453 0.01% 53 2461 0.01% 54 2768 0.01% 55 2927 0.01% 56 3064 0.01% 57 3471 0.01% 58 4120 0.01% 59 4422 0.01% 60 5095 0.01% 61 5858 0.01% 62 6598 0.02% 63 7294 0.02% 64 7627 0.02% 65 8189 0.02% 66 8883 0.02% 67 9818 0.02% 68 10795 0.03% 69 12179 0.03% 70 14005 0.03% 71 15170 0.04% 72 17563 0.04% 73 19282 0.05% 74 21854 0.05% 75 23393 0.06% 76 25475 0.06% 77 27584 0.07% 78 29942 0.07% 79 32197 0.08% 80 35986 0.09% 81 38657 0.09% 82 42651 0.10% 83 47017 0.12% 84 51284 0.13% 85 54953 0.13% 86 58969 0.14% 87 61627 0.15% 88 66556 0.16% 89 69024 0.17% 90 72575 0.18% 91 77309 0.19% 92 81359 0.20% 93 86749 0.21% 94 90957 0.22% 95 95594 0.23% 96 99314 0.24% 97 102214 0.25% 98 104522 0.26% 99 108125 0.27% 100 110578 0.27% 101 112009 0.27% 102 116717 0.29% 103 122488 0.30% 104 123738 0.30% 105 129099 0.32% 106 131077 0.32% 107 132832 0.33% 108 135920 0.33% 109 138728 0.34% 110 139827 0.34% 111 141244 0.35% 112 143371 0.35% 113 145723 0.36% 114 149759 0.37% 115 151254 0.37% 116 153750 0.38% 117 156921 0.39% 118 157252 0.39% 119 158079 0.39% 120 159774 0.39% 121 159984 0.39% 122 160376 0.39% 123 163622 0.40% 124 165709 0.41% 125 167077 0.41% 126 167038 0.41% 127 170860 0.42% 128 170232 0.42% 129 171496 0.42% 130 170665 0.42% 131 171366 0.42% 132 172600 0.42% 133 174281 0.43% 134 175752 0.43% 135 177322 0.44% 136 178472 0.44% 137 176981 0.43% 138 178825 0.44% 139 180037 0.44% 140 179555 0.44% 141 180061 0.44% 142 179899 0.44% 143 179009 0.44% 144 180457 0.44% 145 181163 0.44% 146 179444 0.44% 147 183094 0.45% 148 180993 0.44% 149 181187 0.44% 150 181533 0.45% 151 30787966 75.57% 40739957 reads passed initial QC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=15.75 fanout-score-rank=11 prefix-density=0.17 prefix-fanout=15.8 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCTTATCTCGTATGCCGTCTTCTGCTTGAAAA criterion=fanout-score sequence-density=0.03 sequence-density-rank=38 fanout-score=334.81 fanout-score-rank=1 prefix-density=0.42 prefix-fanout=20.6 sequence=CCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTGGT criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=2.67 fanout-score-rank=36 prefix-density=0.33 prefix-fanout=2.4 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.04 sequence-density-rank=33 fanout-score=449.15 fanout-score-rank=1 prefix-density=1.01 prefix-fanout=18.0 sequence=CGCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGAGGTCATTCAAGTACGCGTGGGTGCTTGACAAGCTGAAGGCTGAGCGTGAGAGAGGTATCACCATCGATATTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGATGCCCCTGGACACCGTGACTTCATCAAGAACATGATTACCGGTACCTCCCAGGCTGACTGTGCCGTGCTTATCATTGACTCCACGACTGGAGGTTTTGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCATGCCCTCCTTGCTTTCACTCTTGGAGTGAAGCAGATGATCTGCTGCTGCAACAAGATGG SRR13165385 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:30:15 Started mapping on | Dec 07 17:30:16 Finished on | Dec 07 18:05:20 Mapping speed, Million of reads per hour | 69.71 Number of input reads | 40739957 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 38487015 Uniquely mapped reads % | 94.47% Average mapped length | 285.37 Number of splices: Total | 35612884 Number of splices: Annotated (sjdb) | 33099891 Number of splices: GT/AG | 35077122 Number of splices: GC/AG | 448564 Number of splices: AT/AC | 26666 Number of splices: Non-canonical | 60532 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.02% Deletion average length | 2.07 Insertion rate per base | 0.01% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 557979 % of reads mapped to multiple loci | 1.37% Number of reads mapped to too many loci | 79733 % of reads mapped to too many loci | 0.20% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.10% % of reads unmapped: other | 0.87% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1695447 1695447 1695447 N_multimapping 557979 557979 557979 N_noFeature 1357687 37384989 1749611 N_ambiguous 833549 4996 123989 UnstrandedReadsAssigned:36295779 PositiveStrandReadsAssigned:1097030 NegativeStrandReadsAssigned:36613415 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=140 echo kmer=135 SRR13165385 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165385-trimmed-pair1.fastq SRR13165385-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 40,739,957 reads, 37,375,918 reads pseudoaligned [quant] estimated average fragment length: 221.407 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,209 rounds 52973 SRR13165385.ke.tsv 35125 SRR13165385.se.tsv 88098 total ==> SRR13165385.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 716.007 0 0 PNS24247 1044 823.593 236.503 11.3078 PNS24249 1928 1707.59 454.557 10.4823 PNS24246 1044 823.593 236.503 11.3078 PNS24248 1044 823.593 236.503 11.3078 PNS24244 1471 1250.59 439.934 13.8524 PNS24243 293 118.064 0 0 KQK14069 1603 1382.59 40037.5 1140.32 KQK14071 474 267.798 779.577 114.632 ==> SRR13165385.se.tsv <== BRADI_1g14170v3 42505 BRADI_1g53295v3 755 BRADI_1g59795v3 1407 BRADI_1g07683v3 0 BRADI_1g00485v3 42 BRADI_1g20270v3 2421 BRADI_1g74790v3 924 BRADI_1g09890v3 0 BRADI_1g77505v3 546 BRADI_1g48960v3 0 SRR13165385 completed mapping pipeline successfully