Starting /dee2/code/volunteer_pipeline.sh SRR13165386 current disk space = 1541338431488 free memory = 1596018548 SRR13165386 SRAfilesize 32c99a1ce7ed126f2ab3ab9e628f167a SRR13165386.sra SRR13165386.sra file validated SRR13165386 is paired end SRR13165386 is conventional basespace SRR13165386 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165386_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5065 37.0 37.0 37.0 37.0 37.0 2 36.23125 37.0 37.0 37.0 37.0 37.0 3 36.5085 37.0 37.0 37.0 37.0 37.0 4 36.4085 37.0 37.0 37.0 37.0 37.0 5 36.523 37.0 37.0 37.0 37.0 37.0 6 36.535 37.0 37.0 37.0 37.0 37.0 7 36.486 37.0 37.0 37.0 37.0 37.0 8 36.5195 37.0 37.0 37.0 37.0 37.0 9 36.536 37.0 37.0 37.0 37.0 37.0 10-14 36.53959999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.4967 37.0 37.0 37.0 37.0 37.0 20-24 36.47769999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.4203 37.0 37.0 37.0 37.0 37.0 30-34 36.4051 37.0 37.0 37.0 37.0 37.0 35-39 36.3517 37.0 37.0 37.0 37.0 37.0 40-44 36.348699999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.2844 37.0 37.0 37.0 37.0 37.0 50-54 36.246900000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.1829 37.0 37.0 37.0 37.0 37.0 60-64 36.164500000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.1186 37.0 37.0 37.0 37.0 37.0 70-74 36.2047 37.0 37.0 37.0 37.0 37.0 75-79 36.2364 37.0 37.0 37.0 37.0 37.0 80-84 36.1534 37.0 37.0 37.0 37.0 37.0 85-89 36.1065 37.0 37.0 37.0 37.0 37.0 90-94 36.0807 37.0 37.0 37.0 37.0 37.0 95-99 36.0972 37.0 37.0 37.0 37.0 37.0 100-104 36.03830000000001 37.0 37.0 37.0 37.0 37.0 105-109 36.0383 37.0 37.0 37.0 37.0 37.0 110-114 36.067 37.0 37.0 37.0 37.0 37.0 115-119 36.0013 37.0 37.0 37.0 37.0 37.0 120-124 35.9043 37.0 37.0 37.0 37.0 37.0 125-129 35.8661 37.0 37.0 37.0 37.0 37.0 130-134 35.9296 37.0 37.0 37.0 37.0 37.0 135-139 35.7666 37.0 37.0 37.0 37.0 37.0 140-144 35.7365 37.0 37.0 37.0 37.0 37.0 145-149 35.5148 37.0 37.0 37.0 37.0 37.0 150-151 35.3035 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 6.0 24 3.0 25 4.0 26 5.0 27 12.0 28 15.0 29 26.0 30 31.0 31 45.0 32 46.0 33 92.0 34 157.0 35 318.0 36 2810.0 37 428.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.05 10.025 5.875 33.050000000000004 2 23.76162936887101 10.56072416394267 31.003268795574552 34.674377671611765 3 21.875 15.1 25.45 37.574999999999996 4 26.825 21.349999999999998 19.8 32.025 5 27.125 26.775 23.95 22.15 6 26.3 29.7 21.05 22.95 7 18.375 26.950000000000003 35.425000000000004 19.25 8 20.175 24.8 29.325000000000003 25.7 9 20.075000000000003 21.875 32.7 25.35 10-14 23.195 27.185 24.52 25.1 15-19 23.400000000000002 25.0 25.95 25.650000000000002 20-24 23.1 25.47 24.995 26.435 25-29 23.885 25.66 24.755 25.7 30-34 23.685000000000002 25.869999999999997 24.39 26.055 35-39 23.055 25.655 25.055 26.235000000000003 40-44 23.255 24.64 25.755 26.35 45-49 23.995 24.185000000000002 25.345000000000002 26.474999999999998 50-54 22.52 25.990000000000002 24.87 26.619999999999997 55-59 24.14 24.765 24.67 26.424999999999997 60-64 24.29 25.35 24.635 25.724999999999998 65-69 23.635 25.540000000000003 24.4 26.424999999999997 70-74 24.759999999999998 24.97 24.365000000000002 25.905 75-79 24.81 24.645 24.15 26.395000000000003 80-84 23.755000000000003 23.855 25.005 27.384999999999998 85-89 24.705 24.565 25.445 25.285000000000004 90-94 24.310000000000002 24.875 24.825 25.990000000000002 95-99 24.46 24.385 24.975 26.179999999999996 100-104 24.529999999999998 24.705 24.779999999999998 25.985000000000003 105-109 24.695 24.895 23.73 26.68 110-114 25.515 25.345000000000002 23.635 25.505 115-119 23.89 24.709999999999997 24.985 26.415 120-124 25.66 24.87 24.085 25.385 125-129 25.040000000000003 24.415 24.33 26.215 130-134 25.11 24.975 23.855 26.06 135-139 24.205 25.424999999999997 23.775 26.595000000000002 140-144 25.11 23.705000000000002 24.03 27.155 145-149 24.73 24.135 23.445 27.689999999999998 150-151 25.724999999999998 24.075 24.0125 26.187500000000004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 0.5 21 0.0 22 0.5 23 1.0 24 1.0 25 0.5 26 0.0 27 0.5 28 3.0 29 3.5 30 4.0 31 5.5 32 7.0 33 11.0 34 15.5 35 27.0 36 50.5 37 60.0 38 54.5 39 72.5 40 105.0 41 123.5 42 150.5 43 178.0 44 193.0 45 211.0 46 221.5 47 212.0 48 188.5 49 181.5 50 167.0 51 159.5 52 159.0 53 138.5 54 124.5 55 102.5 56 85.5 57 83.0 58 81.5 59 72.0 60 72.5 61 61.5 62 48.0 63 57.0 64 57.0 65 51.5 66 53.0 67 56.5 68 45.5 69 29.0 70 34.0 71 38.0 72 27.5 73 24.5 74 19.0 75 15.5 76 17.5 77 15.0 78 10.0 79 5.5 80 3.0 81 2.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.575 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.625 #Duplication Level Percentage of deduplicated Percentage of total 1 71.43897996357012 49.025 2 18.360655737704917 25.2 3 6.448087431693988 13.275 4 2.1129326047358834 5.800000000000001 5 0.8014571948998178 2.75 6 0.4371584699453552 1.7999999999999998 7 0.18214936247723132 0.8750000000000001 8 0.14571948998178508 0.8 9 0.03642987249544627 0.22499999999999998 >10 0.03642987249544627 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTATCGCGTAT 10 0.25 TruSeq Adapter, Index 6 (97% over 37bp) GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 9 0.22499999999999998 No Hit GATCGGCATCCTCTTCTGTATCAAAAAAGCAGAGGCCTCTTGTTCTTCTT 8 0.2 No Hit GGTGAGTCTACTGACAACCACAGACCAGATCAAAAATACGGACAACTGAT 8 0.2 No Hit GCCTTCTCCAATGTCATACTTGTCAACACCATAATTGTATGTCAGTTCCA 8 0.2 No Hit GTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAG 8 0.2 No Hit CCGAGCGCTTCGACCATGGCAAGCACGCAATCTCCTTGACCCCCCAGCAC 7 0.17500000000000002 No Hit CTCCCGGATGTTTTCGCATCATCACCACCTGACTTCCTCTTTTGCTTGGA 7 0.17500000000000002 No Hit GCGCTGATGTACAAGAGGTTATTGCTGCTTGCTCTAGGCAATAGACATGC 7 0.17500000000000002 No Hit ACTGTTCCAAGCTGCCTTTCATGAGATCTTGCTGTGCATGGCTCCTTGTA 7 0.17500000000000002 No Hit ATTCTTATTACAACCACATCCACATTCCACATTCATAACTGCAAACACTG 7 0.17500000000000002 No Hit GCATGACTTGGTTTAGAAATGACTGTTTCAGCTTGTGGTTTGCTTACAGA 6 0.15 No Hit GGCGAAACTTAAATTTGGATAATAGCTGCAGGTTAATTGTGCCGTATGAA 6 0.15 No Hit GTCCTTCCCTGCAGTGCTACCAGCGTATGGATTAACCTGGACATGAGGTC 6 0.15 No Hit GCTTAAAATCTTCTTCATCCAGTGCGGTGTTATGTTGCTCGTTTGCATTG 6 0.15 No Hit CCTCGATCTGGGGGCCCAGCCACTGCGGCGACGTGCGGAGCGCGTACCTG 6 0.15 No Hit GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCG 6 0.15 No Hit GGGAAGGTATCTGGCTATTGTCCAGAGCTAATAGCATTAAACCACTATTT 6 0.15 No Hit CGAGGAGGTCTTTGTTTTCCTTTCTTAAATGAAAGTCCCTCAAGCTCTGC 6 0.15 No Hit GCCCTCGCTAACAGTGTCTTCCCGGTTCCTGGTGGGCCGTAGAGTAGAAC 6 0.15 No Hit CCCAAGCCATCCAGATACGATTTTTCAACAACGGCCTTCCAATGGTTATG 6 0.15 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 6 0.15 No Hit GTTCCTCATCACACGAGCTTTGCTGTCGTCGCCAAGAACCGGCGAATCCC 6 0.15 No Hit GCTGGTTTTCCTTCACCTAACATTGCATTACCTGGAAGCTTTATCCTGTA 5 0.125 No Hit CTTTGATCCCAAAGAGGTTGTATTCTTAGCTTCACGCTTCATGAGAGACT 5 0.125 No Hit ATGGGCTTGTCACCAGGACCAGCAACAACCTTGAAGGGCCAGAGCTTGAT 5 0.125 No Hit GCCAGCATCTGAGTCTTCTCAAGTGGACGGAACACAACTACCTCATCCAT 5 0.125 No Hit GTCCATGGAGACACCGATGGGGGTGCCCTGGAAGTTACCACCGTGGATCG 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTATCTCGTAT 5 0.125 TruSeq Adapter, Index 6 (97% over 37bp) GCACCTTTCTCAGTTTTCCCAATCTTGTAAACATACTATGATTGAGGAAT 5 0.125 No Hit CACACAACTGACTGATCGACTGACTGACTGACTGATTTGATTTGATTTAA 5 0.125 No Hit CCCATTTTTAGTTTCCTTCCAGCATCCTCCATTGTTGATCTCACACCTCC 5 0.125 No Hit ATGCACCAGTTTAATCAGTAACGTTATACATTTCTACATTACAGAGGCTG 5 0.125 No Hit CCCCACCAAAGTTCGCCAAATCTATCGGCAGTATCCTCCCAGGAAATGGT 5 0.125 No Hit CCTCAGGCAAGAACACTGTTCAGAGGAAAGAAACACTGCTCATGGACCCT 5 0.125 No Hit GGGCTGCAGCAGCATCTAACAGCACCGCAGACTCACAAAGCAACTTTGTT 5 0.125 No Hit CTCTGTAATTTCTTTCTTCTACGCCACTGTTGCCATGACCAAGCCAAGGG 5 0.125 No Hit GCCTGAATTGCTAAATGCAAGGTTGCCATTTAGCACCTCTCTTGAGTTTG 5 0.125 No Hit TGATCAACTTCAACACCAACAGCTTCCAAGTTCAACTTATTAGTGTTCGG 5 0.125 No Hit CTTCACAAAATACTGGTACATTCCTGTTAACCCAGTTGTCTGCTCCTGTT 5 0.125 No Hit CCCCACAGCTTATTACCACTACACACAAAACAACAAAGACCTTCAGCCGT 5 0.125 No Hit GGGACACTCGAGCCGGCCTCGCGCTGCGCCGGAGCGGAAGTGGACGCCGC 5 0.125 No Hit CCGTGTATTCAAAGATTTTGGCATCACTGAAACAATGATCAATTTCCTGC 5 0.125 No Hit GGGCGCCGACCGTGGGGAACTCCCGCCGCAATTGAAGATTTCATGACACC 5 0.125 No Hit GCTGTAAGCAGTAGTGACAACATAAGACATGCTTGAGCATAACAGGCTAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.0625 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.16249999999999998 0.0 0.0 0.0 0.0 64-65 0.25 0.0 0.0 0.0 0.0 66-67 0.25 0.0 0.0 0.0 0.0 68-69 0.275 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.275 0.0 0.0 0.0 0.0 74-75 0.3 0.0 0.0 0.0 0.0 76-77 0.325 0.0 0.0 0.0 0.0 78-79 0.4125 0.0 0.0 0.0 0.0 80-81 0.525 0.0 0.0 0.0 0.0 82-83 0.625 0.0 0.0 0.0 0.0 84-85 0.8 0.0 0.0 0.0 0.0 86-87 0.85 0.0 0.0 0.0 0.0 88-89 1.1 0.0 0.0 0.0 0.0 90-91 1.3125 0.0 0.0 0.0 0.0 92-93 1.5125000000000002 0.0 0.0 0.0 0.0 94-95 1.85 0.0 0.0 0.0 0.0 96-97 2.15 0.0 0.0 0.0 0.0 98-99 2.65 0.0 0.0 0.0 0.0 100-101 2.9875 0.0 0.0 0.0 0.0 102-103 3.4875 0.0 0.0 0.0 0.0 104-105 3.7375 0.0 0.0 0.0 0.0 106-107 4.125 0.0 0.0 0.0 0.0 108-109 4.675 0.0 0.0 0.0 0.0 110-111 5.425 0.0 0.0 0.0 0.0 112-113 6.262499999999999 0.0 0.0 0.0 0.0 114-115 6.75 0.0 0.0 0.0 0.0 116-117 7.3375 0.0 0.0 0.0 0.0 118-119 7.9125 0.0 0.0 0.0 0.0 120-121 8.3125 0.0 0.0 0.0 0.0 122-123 9.0625 0.0 0.0 0.0 0.0 124-125 10.0375 0.0 0.0 0.0 0.0 126-127 10.787500000000001 0.0 0.0 0.0 0.0 128-129 11.55 0.0 0.0 0.0 0.0 130-131 12.6125 0.0 0.0 0.0 0.0 132-133 13.287500000000001 0.0 0.0 0.0 0.0 134-135 14.075 0.0 0.0 0.0 0.0 136-137 15.100000000000001 0.0 0.0 0.0 0.0 138-139 15.85 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCGTT 10 0.006830828 145.0 8 >>END_MODULE SRR13165386 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165386_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.15975 37.0 37.0 37.0 37.0 37.0 2 36.242 37.0 37.0 37.0 37.0 37.0 3 36.202 37.0 37.0 37.0 37.0 37.0 4 36.2445 37.0 37.0 37.0 37.0 37.0 5 36.2015 37.0 37.0 37.0 37.0 37.0 6 36.2075 37.0 37.0 37.0 37.0 37.0 7 36.246 37.0 37.0 37.0 37.0 37.0 8 36.308 37.0 37.0 37.0 37.0 37.0 9 36.2735 37.0 37.0 37.0 37.0 37.0 10-14 36.1673 37.0 37.0 37.0 37.0 37.0 15-19 36.106399999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.07 37.0 37.0 37.0 37.0 37.0 25-29 35.952 37.0 37.0 37.0 37.0 37.0 30-34 35.944100000000006 37.0 37.0 37.0 37.0 37.0 35-39 35.936099999999996 37.0 37.0 37.0 37.0 37.0 40-44 35.9638 37.0 37.0 37.0 37.0 37.0 45-49 35.88095 37.0 37.0 37.0 37.0 37.0 50-54 35.831399999999995 37.0 37.0 37.0 37.0 37.0 55-59 35.7909 37.0 37.0 37.0 37.0 37.0 60-64 35.8808 37.0 37.0 37.0 37.0 37.0 65-69 35.815999999999995 37.0 37.0 37.0 37.0 37.0 70-74 35.747699999999995 37.0 37.0 37.0 37.0 37.0 75-79 35.71975 37.0 37.0 37.0 37.0 37.0 80-84 35.812850000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.70675 37.0 37.0 37.0 37.0 37.0 90-94 35.7326 37.0 37.0 37.0 37.0 37.0 95-99 35.7079 37.0 37.0 37.0 37.0 37.0 100-104 35.705 37.0 37.0 37.0 37.0 37.0 105-109 35.677699999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.634299999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.603899999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.563900000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.40235 37.0 37.0 37.0 37.0 37.0 130-134 35.2795 37.0 37.0 37.0 37.0 37.0 135-139 35.239000000000004 37.0 37.0 37.0 34.6 37.0 140-144 35.0378 37.0 37.0 37.0 29.8 37.0 145-149 34.838 37.0 37.0 37.0 25.0 37.0 150-151 34.48625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 4.0 14 8.0 15 5.0 16 6.0 17 1.0 18 3.0 19 0.0 20 4.0 21 8.0 22 7.0 23 12.0 24 11.0 25 7.0 26 11.0 27 10.0 28 14.0 29 18.0 30 26.0 31 46.0 32 68.0 33 112.0 34 208.0 35 482.0 36 2629.0 37 298.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 45.46824002008537 21.81772533266382 7.230730605071554 25.48330404217926 2 30.225 25.2 24.95 19.625 3 25.324999999999996 23.674999999999997 29.099999999999998 21.9 4 27.425 28.825 20.3 23.45 5 29.049999999999997 33.575 18.85 18.525 6 25.324999999999996 35.525 18.475 20.674999999999997 7 22.95 21.6 31.8 23.65 8 24.099999999999998 24.15 25.1 26.650000000000002 9 25.424999999999997 21.825 26.275 26.474999999999998 10-14 27.095000000000002 25.845000000000002 22.66 24.4 15-19 27.01 24.335 24.855 23.799999999999997 20-24 26.040416166466585 24.579831932773107 24.544817927170868 24.834933973589436 25-29 27.392392392392395 24.96996996996997 23.613613613613612 24.024024024024023 30-34 26.758731111778246 25.612929050335236 23.56149304513159 24.06684679275493 35-39 26.780712284913967 25.595238095238095 23.774509803921568 23.84953981592637 40-44 26.93885720004003 24.397077954568196 23.796657660362253 24.86740718502952 45-49 25.924258342088148 25.023763069688325 24.288358597228477 24.763619990995046 50-54 26.285514205682276 24.98999599839936 24.58983593437375 24.134653861544617 55-59 26.231231231231234 25.390390390390387 24.184184184184186 24.194194194194193 60-64 26.94577831132453 24.019607843137255 24.449779911964786 24.58483393357343 65-69 25.69013802760552 25.48509701940388 23.98479695939188 24.83996799359872 70-74 26.891891891891888 25.355355355355357 23.90890890890891 23.843843843843842 75-79 27.070302727045288 25.038779084313234 23.767825869402053 24.12309231923943 80-84 26.996749187296825 25.87146786696674 23.07076769192298 24.06101525381345 85-89 26.96061258195285 25.81952855212452 23.177018167258893 24.04284069866373 90-94 27.213606803401703 25.297648824412207 23.931965982991496 23.556778389194598 95-99 27.240896358543417 24.9749899959984 24.049619847939173 23.734493797519008 100-104 27.912912912912912 26.036036036036037 23.64864864864865 22.4024024024024 105-109 27.652652652652655 24.904904904904903 24.95995995995996 22.48248248248248 110-114 27.78611444577831 25.51520608243297 23.629451780712284 23.06922769107643 115-119 28.298298298298295 25.610610610610614 22.97797797797798 23.113113113113112 120-124 28.453453453453452 25.32032032032032 23.08808808808809 23.138138138138135 125-129 28.053845768903567 24.94620427363259 23.71515788420157 23.284792073262274 130-134 28.863863863863866 24.414414414414416 23.37837837837838 23.343343343343342 135-139 29.404404404404406 24.884884884884883 23.70870870870871 22.002002002002 140-144 30.417166866746697 23.949579831932773 23.684473789515806 21.948779511804723 145-149 31.44144144144144 23.72872872872873 23.1981981981982 21.63163163163163 150-151 32.607607607607605 23.623623623623622 22.985485485485484 20.783283283283282 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 0.5 12 0.5 13 0.5 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 0.5 24 1.0 25 2.0 26 3.0 27 2.0 28 3.0 29 3.0 30 5.0 31 9.5 32 15.0 33 18.5 34 18.5 35 31.5 36 44.5 37 52.0 38 62.0 39 78.5 40 110.5 41 128.5 42 134.5 43 147.0 44 176.5 45 202.0 46 205.0 47 189.5 48 178.5 49 172.0 50 165.5 51 172.0 52 158.5 53 129.0 54 107.5 55 91.5 56 81.5 57 74.0 58 70.0 59 79.5 60 83.5 61 85.5 62 77.0 63 63.0 64 64.5 65 68.5 66 56.0 67 42.5 68 49.5 69 40.5 70 23.0 71 27.5 72 30.5 73 23.5 74 24.0 75 25.0 76 18.5 77 13.0 78 8.0 79 6.5 80 5.5 81 2.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 1.0 88 1.0 89 2.0 90 2.0 91 1.0 92 0.5 93 0.5 94 2.5 95 4.0 96 4.0 97 3.0 98 2.0 99 3.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.04 25-29 0.1 30-34 0.06999999999999999 35-39 0.04 40-44 0.06999999999999999 45-49 0.055 50-54 0.04 55-59 0.1 60-64 0.04 65-69 0.02 70-74 0.1 75-79 0.075 80-84 0.025 85-89 0.095 90-94 0.05 95-99 0.04 100-104 0.1 105-109 0.1 110-114 0.04 115-119 0.1 120-124 0.1 125-129 0.08499999999999999 130-134 0.1 135-139 0.1 140-144 0.04 145-149 0.1 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.85 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69148174659986 50.775000000000006 2 17.609162491052256 24.6 3 6.1560486757337145 12.9 4 2.0042949176807445 5.6000000000000005 5 0.7874015748031495 2.75 6 0.5010737294201861 2.1 7 0.17895490336435219 0.8750000000000001 8 0.07158196134574087 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAGGAGCCACTGACCCGACATTCCTGTATTTCGCGCATGGGCTTAAGGA 8 0.2 No Hit CCATCGCATTGCCCTGCGGCCTCCGTCCGCCTCGAGACTCTTCTCGTCTC 8 0.2 No Hit AAAGGATGTTCGCTTCTTTGTATTCTGGGGCCTACGCTATCAATCCCTTT 7 0.17500000000000002 No Hit GTTTGAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTT 7 0.17500000000000002 No Hit GAATTAAAGAAGCTGCTTGGGCGTGCTTCTGGACAAAATGAGTCAGATGC 7 0.17500000000000002 No Hit AAGCTTCCCTCTCCACCTTCTCTCTACGCGGAGTCTCTTCCTCCCACTGC 7 0.17500000000000002 No Hit GGAGAGGGTTGTCCAAGTACTACCAGGGAAAGTCCCAATCATTCACATCA 7 0.17500000000000002 No Hit GATAACCAGAACAACCCCCTGGCGGTGACCCAGTTGAAGCTTGCACAAAT 6 0.15 No Hit AGTGGTCCAAGATACGTTGTCGGGTGCCGGAGTAAAGTGGACAAGGAGAA 6 0.15 No Hit TGAACAAGTGCAGACTCTTGTAAGCTTTGATATCTCAAGTACCAATATGA 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 6 0.15 No Hit AAACTCATAACTGCTGATAAGCATATAGTGAGAGTTTGGGACCCTAATAC 6 0.15 No Hit CTGCTCGTCTACTCGCGCTGTTGATGCACCGGGGCTTTAAGTTCTCCATC 6 0.15 No Hit GTTCTGGTGATCAGCGTGCACAGGATATTCTGAGACTGATGACAACTTAT 6 0.15 No Hit CCCTGGCTCCATCGAGGCCGCGGCCATCATGGAGCACATCCTGGCTGGGA 6 0.15 No Hit CCGCAAGCAGATCGAACGCGAGATGGAACGATTTAAGGTCTGTGAGAAGG 6 0.15 No Hit GAGGTAGTGAGTGGCCTGAAGAGTGGCCCAAGAGGCTCGAGACTTTTCCT 6 0.15 No Hit GTCAAATTTTCACTTGTGGATTTGTATGGCTAGAAACCTGATGCACTATG 6 0.15 No Hit GGGAAGGGAAGAACACAGAAGCTGAAGAGGAACTGGAGAAGGCTAAGGTC 6 0.15 No Hit GCCGACGAGCCGCGTCCGGCCGCCTCGAAGCTCCCTTCCCCACGGGCGGC 6 0.15 No Hit GTTCATTTCCAGCAAACCATAAGCCCTGACCAGAAACTCGTTGGGCGCAG 6 0.15 No Hit CGTCGCCCAAGTAGATCGGTCTTATACCAAGGGAAAATTGTAACACTGTA 5 0.125 No Hit GACTGTGAAACCAGGTACAATGTGCTACATGTCTGGGAACATTCAGATGG 5 0.125 No Hit GCGCCGCCGTATAAGCTCGAGGCTGTCGTTGATCAATGCCCCCGGAATGA 5 0.125 No Hit AAACAATTATTGAGAATGTAAGACCTCGAAACCTCTTGTCCAGTATTATC 5 0.125 No Hit TGGGCTGCAGAGCCAAGTAGCATCACTAGCTAGCAACCCCCTCCCAGTGA 5 0.125 No Hit GACATCAAATGTTGGTTCTGCATCGATTTCCAAGGGAAGGATGAGCATAG 5 0.125 No Hit AGTAGACAAGGACAGCAGCACGGAAAGAGAGGAGAGATCGGAGAGAGGTC 5 0.125 No Hit ACATGGAATGCACTCCTCGTTAGAATCATCCATTGATAAGGGCCCATATG 5 0.125 No Hit GGCGAACATTCAGGCTATCCTTGCTGAGGTCCTGTCTGCCGTGTTCTGCG 5 0.125 No Hit CGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATT 5 0.125 No Hit ATGGCATTAAAGTCGTGACAGATAAAGGAGATGAGCTCATGGCAGATGTT 5 0.125 No Hit AAACACATTGCCATACTTGGAAAATGAATCAGGCCTCCACTTGAAGATAA 5 0.125 No Hit AAAGTAGCTGGGAATTTTCACTTTGCCCCAGGAAAACACTTGGACCAATC 5 0.125 No Hit AACAGCACATCCAGTGAAAGCAGAAATCGGTTGCGTGATGCCTGTCGCCT 5 0.125 No Hit GGTTGAATTTGAATCCTCGCCGTTGTTTCAAGGGGATGAGTGAGTGAATC 5 0.125 No Hit GACCACTGGATTTTCTGGGTGGGTCCGTTCATTGGAGCCGCGCTGGCTGC 5 0.125 No Hit AGAGAGATTTCAAGTTCGCCGGAGCTAGAGAGAGATGGCGGCTAAGGGCG 5 0.125 No Hit CAGCCTCCTCCTATCGCCCCCGTCAAGGAAAGGCTTCTCCCTTTCCTCCT 5 0.125 No Hit GCCACCACTCTCCACCCGCAATTCCGGCCACCGCCGCATACGCGCGCACC 5 0.125 No Hit CTCAAGTTTATCAAAGGTTCTCTTGAAATTAAGCTTACAGCAGTTGCCTC 5 0.125 No Hit TGCCACCACGTCGGTGAGGACCGAGAGACCGCCTACGACGGTTTTGAGTG 5 0.125 No Hit GAGAACAATGGCGGTTGCTGGCTTGACAACGATAATAACGTCACTGCTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.0625 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.16249999999999998 0.0 0.0 0.0 0.0 64-65 0.25 0.0 0.0 0.0 0.0 66-67 0.25 0.0 0.0 0.0 0.0 68-69 0.275 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.275 0.0 0.0 0.0 0.0 74-75 0.3125 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.4375 0.0 0.0 0.0 0.0 80-81 0.55 0.0 0.0 0.0 0.0 82-83 0.6499999999999999 0.0 0.0 0.0 0.0 84-85 0.825 0.0 0.0 0.0 0.0 86-87 0.875 0.0 0.0 0.0 0.0 88-89 1.125 0.0 0.0 0.0 0.0 90-91 1.3375 0.0 0.0 0.0 0.0 92-93 1.5375 0.0 0.0 0.0 0.0 94-95 1.875 0.0 0.0 0.0 0.0 96-97 2.1624999999999996 0.0 0.0 0.0 0.0 98-99 2.65 0.0 0.0 0.0 0.0 100-101 2.9875 0.0 0.0 0.0 0.0 102-103 3.475 0.0 0.0 0.0 0.0 104-105 3.725 0.0 0.0 0.0 0.0 106-107 4.175 0.0 0.0 0.0 0.0 108-109 4.725 0.0 0.0 0.0 0.0 110-111 5.45 0.0 0.0 0.0 0.0 112-113 6.2875 0.0 0.0 0.0 0.0 114-115 6.775 0.0 0.0 0.0 0.0 116-117 7.362500000000001 0.0 0.0 0.0 0.0 118-119 7.949999999999999 0.0 0.0 0.0 0.0 120-121 8.3375 0.0 0.0 0.0 0.0 122-123 9.0625 0.0 0.0 0.0 0.0 124-125 10.05 0.0 0.0 0.0 0.0 126-127 10.837499999999999 0.0 0.0 0.0 0.0 128-129 11.587499999999999 0.0 0.0 0.0 0.0 130-131 12.6125 0.0 0.0 0.0 0.0 132-133 13.3125 0.0 0.0 0.0 0.0 134-135 14.100000000000001 0.0 0.0 0.0 0.0 136-137 15.1375 0.0 0.0 0.0 0.0 138-139 15.899999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532112 spots for SRR13165386.sra Written 1532112 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra Read 1532108 spots for SRR13165386.sra Written 1532108 spots for SRR13165386.sra SRR ids: ['SRR13165386.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_qlr4uhc9 SRR13165386.sra spots: 30642164 blocks: [[1, 1532108], [1532109, 3064216], [3064217, 4596324], [4596325, 6128432], [6128433, 7660540], [7660541, 9192648], [9192649, 10724756], [10724757, 12256864], [12256865, 13788972], [13788973, 15321080], [15321081, 16853188], [16853189, 18385296], [18385297, 19917404], [19917405, 21449512], [21449513, 22981620], [22981621, 24513728], [24513729, 26045836], [26045837, 27577944], [27577945, 29110052], [29110053, 30642164]] SRR13165386 file size 10391847 SRR13165386 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165386 SRR13165386_1.fastq SRR13165386_2.fastq Input file: SRR13165386_1.fastq Paired file: SRR13165386_2.fastq trimmed: SRR13165386-trimmed-pair1.fastq, SRR13165386-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 17:18:45 2024 >> started Sat Dec 7 17:19:22 2024 >> done (36.722s) 30642164 read pairs processed; of these: 629 ( 0.00%) short read pairs filtered out after trimming by size control 130247 ( 0.43%) empty read pairs filtered out after trimming by size control 30511288 (99.57%) read pairs available; of these: 6171980 (20.23%) trimmed read pairs available after processing 24339308 (79.77%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 40 0.00% 19 43 0.00% 20 46 0.00% 21 81 0.00% 22 70 0.00% 23 86 0.00% 24 90 0.00% 25 85 0.00% 26 118 0.00% 27 150 0.00% 28 138 0.00% 29 171 0.00% 30 206 0.00% 31 195 0.00% 32 242 0.00% 33 235 0.00% 34 256 0.00% 35 238 0.00% 36 261 0.00% 37 229 0.00% 38 282 0.00% 39 335 0.00% 40 372 0.00% 41 373 0.00% 42 384 0.00% 43 383 0.00% 44 406 0.00% 45 435 0.00% 46 458 0.00% 47 646 0.00% 48 614 0.00% 49 710 0.00% 50 870 0.00% 51 926 0.00% 52 945 0.00% 53 1110 0.00% 54 1155 0.00% 55 1148 0.00% 56 1441 0.00% 57 1617 0.01% 58 1834 0.01% 59 2048 0.01% 60 2230 0.01% 61 2544 0.01% 62 2856 0.01% 63 3242 0.01% 64 3437 0.01% 65 3930 0.01% 66 4281 0.01% 67 4477 0.01% 68 5037 0.02% 69 5740 0.02% 70 6269 0.02% 71 6974 0.02% 72 8276 0.03% 73 9037 0.03% 74 10188 0.03% 75 11197 0.04% 76 12224 0.04% 77 13421 0.04% 78 14392 0.05% 79 15944 0.05% 80 16982 0.06% 81 18719 0.06% 82 20882 0.07% 83 23606 0.08% 84 25493 0.08% 85 28196 0.09% 86 30793 0.10% 87 32128 0.11% 88 33847 0.11% 89 35309 0.12% 90 37387 0.12% 91 40072 0.13% 92 42893 0.14% 93 45942 0.15% 94 48609 0.16% 95 51880 0.17% 96 54595 0.18% 97 56766 0.19% 98 58995 0.19% 99 62491 0.20% 100 63140 0.21% 101 64254 0.21% 102 65437 0.21% 103 68316 0.22% 104 70990 0.23% 105 72391 0.24% 106 74753 0.25% 107 76946 0.25% 108 79216 0.26% 109 80682 0.26% 110 81426 0.27% 111 83587 0.27% 112 84951 0.28% 113 85963 0.28% 114 89352 0.29% 115 91521 0.30% 116 94101 0.31% 117 94661 0.31% 118 96425 0.32% 119 97593 0.32% 120 100682 0.33% 121 100125 0.33% 122 101725 0.33% 123 103579 0.34% 124 105815 0.35% 125 106682 0.35% 126 108723 0.36% 127 108751 0.36% 128 109979 0.36% 129 112757 0.37% 130 112546 0.37% 131 113014 0.37% 132 113704 0.37% 133 115647 0.38% 134 115883 0.38% 135 118006 0.39% 136 118392 0.39% 137 119527 0.39% 138 120695 0.40% 139 121169 0.40% 140 122200 0.40% 141 124901 0.41% 142 124882 0.41% 143 125449 0.41% 144 127438 0.42% 145 127658 0.42% 146 127286 0.42% 147 128655 0.42% 148 128693 0.42% 149 129816 0.43% 150 129143 0.42% 151 24339308 79.77% 30511288 reads passed initial QC criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=5.93 fanout-score-rank=24 prefix-density=0.31 prefix-fanout=4.2 sequence=TGCAGTTGTCGC criterion=fanout-score sequence-density=0.11 sequence-density-rank=12 fanout-score=81.21 fanout-score-rank=1 prefix-density=0.64 prefix-fanout=13.5 sequence=TTCTTCTTCTTCC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=2.19 fanout-score-rank=33 prefix-density=0.24 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=155.52 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=8.1 sequence=ACCAAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGCCGACCCTGATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCATTACGAGTTCTATCAGGTAAAGCCAATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTCCGGTGAGCCGCGCCATGGAATCGGGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCG SRR13165386 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:20:10 Started mapping on | Dec 07 17:20:10 Finished on | Dec 07 17:22:59 Mapping speed, Million of reads per hour | 649.94 Number of input reads | 30511288 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 26946584 Uniquely mapped reads % | 88.32% Average mapped length | 289.15 Number of splices: Total | 25063678 Number of splices: Annotated (sjdb) | 23171910 Number of splices: GT/AG | 24676064 Number of splices: GC/AG | 330688 Number of splices: AT/AC | 14869 Number of splices: Non-canonical | 42057 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.39 Insertion rate per base | 0.01% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 341308 % of reads mapped to multiple loci | 1.12% Number of reads mapped to too many loci | 411533 % of reads mapped to too many loci | 1.35% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.77% % of reads unmapped: other | 6.45% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3223706 3223706 3223706 N_multimapping 341308 341308 341308 N_noFeature 1186910 26120945 1470238 N_ambiguous 642416 3471 100311 UnstrandedReadsAssigned:25117258 PositiveStrandReadsAssigned:822168 NegativeStrandReadsAssigned:25376035 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=150 echo kmer=145 SRR13165386 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165386-trimmed-pair1.fastq SRR13165386-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,511,288 reads, 25,811,749 reads pseudoaligned [quant] estimated average fragment length: 235.97 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,312 rounds 52973 SRR13165386.ke.tsv 35125 SRR13165386.se.tsv 88098 total ==> SRR13165386.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 701.551 0 0 PNS24247 1044 809.03 141.354 10.139 PNS24249 1928 1693.03 223.007 7.64374 PNS24246 1044 809.03 141.354 10.139 PNS24248 1044 809.03 141.354 10.139 PNS24244 1471 1236.03 247.932 11.6401 PNS24243 293 110.943 2 1.04612 KQK14069 1603 1368.03 42243.7 1791.92 KQK14071 474 257.077 628.729 141.923 ==> SRR13165386.se.tsv <== BRADI_1g14170v3 45070 BRADI_1g53295v3 622 BRADI_1g59795v3 1268 BRADI_1g07683v3 0 BRADI_1g00485v3 22 BRADI_1g20270v3 716 BRADI_1g74790v3 764 BRADI_1g09890v3 0 BRADI_1g77505v3 447 BRADI_1g48960v3 0 SRR13165386 completed mapping pipeline successfully