Starting /dee2/code/volunteer_pipeline.sh SRR13165387 current disk space = 1541308997632 free memory = 1602363076 SRR13165387 SRAfilesize fdbcfb08166259c831ce207c565a8c47 SRR13165387.sra SRR13165387.sra file validated SRR13165387 is paired end SRR13165387 is conventional basespace SRR13165387 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165387_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5805 37.0 37.0 37.0 37.0 37.0 2 36.1565 37.0 37.0 37.0 37.0 37.0 3 36.5875 37.0 37.0 37.0 37.0 37.0 4 36.5925 37.0 37.0 37.0 37.0 37.0 5 36.5395 37.0 37.0 37.0 37.0 37.0 6 36.5975 37.0 37.0 37.0 37.0 37.0 7 36.4955 37.0 37.0 37.0 37.0 37.0 8 36.567 37.0 37.0 37.0 37.0 37.0 9 36.518 37.0 37.0 37.0 37.0 37.0 10-14 36.553399999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.4964 37.0 37.0 37.0 37.0 37.0 20-24 36.4894 37.0 37.0 37.0 37.0 37.0 25-29 36.4234 37.0 37.0 37.0 37.0 37.0 30-34 36.427800000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.2875 37.0 37.0 37.0 37.0 37.0 40-44 36.2177 37.0 37.0 37.0 37.0 37.0 45-49 34.23799999999999 37.0 37.0 37.0 21.4 37.0 50-54 34.651 37.0 37.0 37.0 24.6 37.0 55-59 33.323 37.0 37.0 37.0 13.8 37.0 60-64 33.5907 37.0 37.0 37.0 16.6 37.0 65-69 33.3166 37.0 37.0 37.0 13.8 37.0 70-74 34.4563 37.0 37.0 37.0 24.6 37.0 75-79 36.0524 37.0 37.0 37.0 37.0 37.0 80-84 36.2077 37.0 37.0 37.0 37.0 37.0 85-89 36.2468 37.0 37.0 37.0 37.0 37.0 90-94 36.1583 37.0 37.0 37.0 37.0 37.0 95-99 36.090700000000005 37.0 37.0 37.0 37.0 37.0 100-104 36.0665 37.0 37.0 37.0 37.0 37.0 105-109 36.062799999999996 37.0 37.0 37.0 37.0 37.0 110-114 36.0245 37.0 37.0 37.0 37.0 37.0 115-119 35.892100000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.80219999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.668899999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.5837 37.0 37.0 37.0 37.0 37.0 135-139 35.375699999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.1962 37.0 37.0 37.0 34.6 37.0 145-149 34.8221 37.0 37.0 37.0 25.0 37.0 150-151 34.69125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 1.0 23 5.0 24 4.0 25 6.0 26 10.0 27 16.0 28 19.0 29 31.0 30 30.0 31 51.0 32 126.0 33 542.0 34 190.0 35 355.0 36 2245.0 37 367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.175000000000004 9.0 7.5 35.325 2 23.26283987915408 22.80966767371601 25.50352467270896 28.423967774420944 3 19.175 13.025 33.75 34.050000000000004 4 23.025000000000002 19.575 17.05 40.35 5 37.525 24.6 18.2 19.675 6 36.9 24.349999999999998 19.55 19.2 7 16.05 35.175 30.825000000000003 17.95 8 21.45 32.775 22.675 23.1 9 32.45 18.2 26.450000000000003 22.900000000000002 10-14 23.035 27.915 21.46 27.589999999999996 15-19 23.68 24.04 24.169999999999998 28.110000000000003 20-24 23.46 26.605 23.87 26.064999999999998 25-29 23.765 24.685000000000002 23.544999999999998 28.005000000000003 30-34 20.845 23.875 23.66 31.619999999999997 35-39 25.919999999999998 27.279999999999998 23.580000000000002 23.22 40-44 21.425 23.810000000000002 25.82 28.945 45-49 25.31 23.585 25.7 25.405 50-54 26.14 21.89 23.635 28.335 55-59 23.189999999999998 21.215 28.16 27.435 60-64 26.445 21.11 26.6 25.845000000000002 65-69 25.365 30.205 21.485000000000003 22.945 70-74 35.589999999999996 21.325 20.325 22.759999999999998 75-79 35.785 21.09 19.555 23.57 80-84 35.555 21.525 19.41 23.51 85-89 36.05 21.02 19.994999999999997 22.935 90-94 35.94 20.91 20.325 22.825 95-99 34.86 22.43 19.805 22.905 100-104 36.0 21.165 19.37 23.465 105-109 36.42 21.315 19.54 22.725 110-114 35.855 21.36 19.21 23.575 115-119 36.28 21.445 19.585 22.689999999999998 120-124 35.615 21.51 19.34 23.535 125-129 35.83 21.27 19.37 23.53 130-134 36.155 20.51 19.509999999999998 23.825 135-139 37.059999999999995 20.815 20.115 22.009999999999998 140-144 36.025 20.25 19.634999999999998 24.09 145-149 37.92 19.93 19.84 22.31 150-151 37.5375 19.4625 20.4375 22.5625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 0.5 28 1.0 29 4.0 30 3.0 31 5.0 32 9.0 33 13.5 34 22.0 35 20.5 36 23.5 37 36.5 38 46.0 39 69.5 40 79.0 41 87.0 42 110.5 43 118.0 44 127.0 45 140.5 46 148.5 47 162.5 48 169.5 49 167.0 50 163.0 51 152.5 52 141.0 53 116.0 54 104.5 55 109.0 56 95.5 57 88.0 58 68.0 59 54.0 60 74.0 61 70.5 62 66.0 63 65.0 64 66.0 65 103.5 66 172.0 67 196.0 68 151.5 69 98.0 70 59.0 71 35.0 72 34.5 73 34.0 74 24.0 75 24.5 76 20.0 77 12.0 78 11.5 79 9.5 80 3.0 81 4.0 82 5.0 83 2.5 84 1.0 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.7000000000000001 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.475 #Duplication Level Percentage of deduplicated Percentage of total 1 73.037820252135 44.9 2 16.998779991866613 20.9 3 6.10004066693778 11.25 4 2.1146807645384302 5.2 5 0.9760065067100446 3.0 6 0.24400162667751116 0.8999999999999999 7 0.16266775111834078 0.7000000000000001 8 0.040666937779585195 0.2 9 0.0 0.0 >10 0.20333468889792597 2.6 >50 0.040666937779585195 1.3 >100 0.08133387555917039 9.049999999999999 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCTCGTAT 253 6.325 TruSeq Adapter, Index 7 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCGCGTAT 109 2.725 TruSeq Adapter, Index 7 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCTCGTTT 52 1.3 TruSeq Adapter, Index 7 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCGCGTTT 38 0.95 TruSeq Adapter, Index 7 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCTCGGAT 23 0.575 TruSeq Adapter, Index 7 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCGCGGAT 16 0.4 TruSeq Adapter, Index 7 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCTCGGTT 14 0.35000000000000003 TruSeq Adapter, Index 7 (97% over 38bp) CGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCTCGTATGCC 13 0.325 TruSeq Adapter, Index 7 (97% over 35bp) TGCTACGACACTTGTATCGGCCTACTTCTGCAAGTTTTTCCTGCCTTTGC 8 0.2 No Hit CCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCA 7 0.17500000000000002 No Hit CTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCA 7 0.17500000000000002 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 7 0.17500000000000002 No Hit TCACCGCGTTCTCTCCGCTCGCCCTGGCGACGAGCTGGGCGCCGAGGACA 7 0.17500000000000002 No Hit CCTTGATCGAGCGAGGCATGAGGAAGGACATGGATCGTGTCGGATGAACA 6 0.15 No Hit CTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTATCATCGCGGTT 6 0.15 TruSeq Adapter, Index 7 (97% over 38bp) ATCCCAGGTAAAACAGAAAGGGGAGTGGAAACAGACAACAGGCTAACACT 6 0.15 No Hit CCTCAGAGCCCAGGGAGTTGGCGCCGCCGAGGTGCTGCCCCTCCTCGCCG 6 0.15 No Hit CACAGAACCTGGATGCTAAAAATCAGAAGCTCTCATCACATCGCACGGAA 6 0.15 No Hit GCCCTACATCACATGTTCCACAACCCAAGCAATAACTAACTCCCCAAAAA 5 0.125 No Hit CACCAAAACTGCCATGAAATGGACCATGGCATGGCAACAGCATGGACTGA 5 0.125 No Hit GGCACAAGAGTTTTCTGTTATTTCTTACCAACACTTAGGTTAGACAAAGG 5 0.125 No Hit GTGCTCGTATTTGCCACTCCACCTCTTGAAATCATCAGATGGGACTTGGA 5 0.125 No Hit CTTCTGCTATTGTACAAATCATTGTTTTCTTTTCTATTTTACACAGATGT 5 0.125 No Hit GCCCGCGCGGAGGCTGGCCCCCTCGATCTTCACCACGGGCTCCCGGAACT 5 0.125 No Hit CAACAATGCTGCTTCAGTTGGTGTGCCCAAGATGGTGTTTTTGCCGTCAT 5 0.125 No Hit ATGCAAAACAACAACAATGGTAGAAACAGCTTACACGGCATAACAGCGTA 5 0.125 No Hit CCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACTG 5 0.125 No Hit GCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGAT 5 0.125 No Hit CGTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCT 5 0.125 No Hit CTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCGGGCCGGACGGGGCTC 5 0.125 No Hit GGCAGACGAAAGAAACAACATATGCGACAAAAAGCCAAAGCTTCACCCTC 5 0.125 No Hit GTCGGAGAGGGGTGGTGGTGGTTACCGGCCCCGCCGACGGTGACGACGGA 5 0.125 No Hit AGCATAACAGATGTGGAAGGACAATGTGTAATGCTAGCTATCAGCTTCAC 5 0.125 No Hit CCTTCCTTGGCTTGTGGCAGAGCATGTCGGGCGGGATCACGTAGAGGTCC 5 0.125 No Hit GAAACTATGGTAAATCTGAACCCCAGGTAGATGTACCATGAAGTGCACTG 5 0.125 No Hit GGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGC 5 0.125 No Hit CACCTATGTGCAGCAAAAAGGAGGACATCTGCACAGGAACTGTTCATCTT 5 0.125 No Hit CCGAGGCCCCCGCCGGCGTCTCCGGACTTCCTAACGTCGCCGTCAACCGC 5 0.125 No Hit CGCCATTATTGCTCTCGAGTCCCCAAGATTTCCAACTACAAGATCCCATC 5 0.125 No Hit GCTCGAGATCACTTGTATCAACATTGACACCAAGTGGAACAACAATCCTG 5 0.125 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 5 0.125 No Hit CGCCAGTAGACCTGCCAAACTGTCATTATCCCAGAATATACCAGATGAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0125 16-17 0.0 0.0 0.0 0.0 0.025 18-19 0.0 0.0 0.0 0.0 0.025 20-21 0.0 0.0 0.0 0.0 0.025 22-23 0.0 0.0 0.0 0.0 0.025 24-25 0.0 0.0 0.0 0.0 0.025 26-27 0.0 0.0 0.0 0.0 0.025 28-29 0.0 0.0 0.0 0.0 0.025 30-31 0.0 0.0 0.0 0.0 0.025 32-33 0.0 0.0 0.0 0.0 0.025 34-35 0.0 0.0 0.0 0.0 0.025 36-37 0.025 0.0 0.0 0.0 0.025 38-39 0.025 0.0 0.0 0.0 0.025 40-41 0.025 0.0 0.0 0.0 0.025 42-43 0.075 0.0 0.0 0.0 0.025 44-45 0.075 0.0 0.0 0.0 0.025 46-47 0.0875 0.0 0.0 0.0 0.025 48-49 0.125 0.0 0.0 0.0 0.025 50-51 0.15 0.0 0.0 0.0 0.025 52-53 0.225 0.0 0.0 0.0 0.025 54-55 0.275 0.0 0.0 0.0 0.025 56-57 0.4125 0.0 0.0 0.0 0.025 58-59 0.4625 0.0 0.0 0.0 0.025 60-61 0.5 0.0 0.0 0.0 0.025 62-63 0.5875 0.0 0.0 0.0 0.025 64-65 0.75 0.0 0.0 0.0 0.025 66-67 0.8625 0.0 0.0 0.0 0.025 68-69 1.0 0.0 0.0 0.0 0.025 70-71 1.2374999999999998 0.0 0.0 0.0 0.025 72-73 1.4874999999999998 0.0 0.0 0.0 0.025 74-75 1.7375 0.0 0.0 0.0 0.025 76-77 1.9625 0.0 0.0 0.0 0.025 78-79 2.3 0.0 0.0 0.0 0.025 80-81 2.725 0.0 0.0 0.0 0.025 82-83 2.9875 0.0 0.0 0.0 0.025 84-85 3.3375 0.0 0.0 0.0 0.025 86-87 3.95 0.0 0.0 0.0 0.025 88-89 4.824999999999999 0.0 0.0 0.0 0.025 90-91 5.2125 0.0 0.0 0.0 0.025 92-93 5.725 0.0 0.0 0.0 0.025 94-95 6.1 0.0 0.0 0.0 0.025 96-97 6.625 0.0 0.0 0.0 0.025 98-99 7.6 0.0 0.0 0.0 0.025 100-101 8.8125 0.0 0.0 0.0 0.025 102-103 9.4875 0.0 0.0 0.0 0.025 104-105 10.2 0.0 0.0 0.0 0.025 106-107 10.712499999999999 0.0 0.0 0.0 0.025 108-109 11.5875 0.0 0.0 0.0 0.025 110-111 12.5 0.0 0.0 0.0 0.025 112-113 13.287500000000001 0.0 0.0 0.0 0.025 114-115 13.975 0.0 0.0 0.0 0.025 116-117 14.8125 0.0 0.0 0.0 0.025 118-119 15.8625 0.0 0.0 0.0 0.025 120-121 16.85 0.0 0.0 0.0 0.025 122-123 17.575 0.0 0.0 0.0 0.025 124-125 18.2 0.0 0.0 0.0 0.025 126-127 19.5125 0.0 0.0 0.0 0.025 128-129 20.425 0.0 0.0 0.0 0.025 130-131 21.1 0.0 0.0 0.0 0.025 132-133 21.9375 0.0 0.0 0.0 0.025 134-135 22.55 0.0 0.0 0.0 0.025 136-137 23.525 0.0 0.0 0.0 0.025 138-139 24.5375 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACTTG 10 0.006830828 145.0 9 GAGCACA 185 0.0 78.37837 9 GATCGGA 195 0.0 74.35898 1 TCGGAAG 195 0.0 74.35898 3 AAGAGCA 200 0.0 72.5 7 GAAGAGC 200 0.0 72.5 6 CGGAAGA 200 0.0 72.5 4 ATCGGAA 200 0.0 72.5 2 GGAAGAG 200 0.0 72.5 5 AGAGCAC 210 0.0 69.047615 8 ATGGGGG 25 4.977651E-4 29.0 65-69 AATGGGG 25 4.977651E-4 29.0 65-69 AAATGGG 20 0.00593511 29.0 65-69 TTTGCCG 20 0.00593511 29.0 45-49 AAGGGGG 55 5.9590093E-9 26.363636 65-69 AAAGGGG 45 8.383813E-7 25.777777 65-69 CTGCTTG 40 9.990927E-6 25.375 55-59 TCTGCTT 35 1.1966578E-4 24.857143 55-59 GCTTGAA 35 1.1966578E-4 24.857143 60-64 AGGGGGG 60 1.5050318E-8 24.166668 65-69 >>END_MODULE SRR13165387 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165387_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.057 37.0 37.0 37.0 37.0 37.0 2 36.201 37.0 37.0 37.0 37.0 37.0 3 35.956 37.0 37.0 37.0 37.0 37.0 4 36.019 37.0 37.0 37.0 37.0 37.0 5 36.0155 37.0 37.0 37.0 37.0 37.0 6 36.1755 37.0 37.0 37.0 37.0 37.0 7 35.7055 37.0 37.0 37.0 37.0 37.0 8 35.28 37.0 37.0 37.0 37.0 37.0 9 35.232 37.0 37.0 37.0 25.0 37.0 10-14 34.8526 37.0 37.0 37.0 25.0 37.0 15-19 34.8115 37.0 37.0 37.0 25.0 37.0 20-24 34.65975 37.0 37.0 37.0 25.0 37.0 25-29 33.558049999999994 37.0 37.0 37.0 13.8 37.0 30-34 33.25345 37.0 37.0 37.0 11.0 37.0 35-39 33.08115 37.0 37.0 37.0 11.0 37.0 40-44 33.14995 37.0 37.0 37.0 11.0 37.0 45-49 33.25835 37.0 37.0 37.0 13.8 37.0 50-54 33.820550000000004 37.0 37.0 37.0 19.4 37.0 55-59 34.37645 37.0 37.0 37.0 25.0 37.0 60-64 34.61105 37.0 37.0 37.0 25.0 37.0 65-69 34.46965 37.0 37.0 37.0 25.0 37.0 70-74 33.72855 37.0 37.0 37.0 22.2 37.0 75-79 33.165350000000004 37.0 37.0 37.0 13.8 37.0 80-84 33.57025 37.0 37.0 37.0 22.2 37.0 85-89 33.91994999999999 37.0 37.0 37.0 25.0 37.0 90-94 34.572050000000004 37.0 37.0 37.0 25.0 37.0 95-99 34.955349999999996 37.0 37.0 37.0 25.0 37.0 100-104 35.08215 37.0 37.0 37.0 27.4 37.0 105-109 35.18965000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.15315 37.0 37.0 37.0 32.2 37.0 115-119 35.11955 37.0 37.0 37.0 27.4 37.0 120-124 34.99875 37.0 37.0 37.0 25.0 37.0 125-129 34.935249999999996 37.0 37.0 37.0 25.0 37.0 130-134 34.74765 37.0 37.0 37.0 25.0 37.0 135-139 34.6276 37.0 37.0 37.0 25.0 37.0 140-144 34.39555 37.0 37.0 37.0 25.0 37.0 145-149 34.16725 37.0 37.0 37.0 25.0 37.0 150-151 33.98625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 5.0 13 9.0 14 11.0 15 9.0 16 11.0 17 12.0 18 12.0 19 18.0 20 23.0 21 12.0 22 34.0 23 33.0 24 30.0 25 43.0 26 30.0 27 44.0 28 64.0 29 88.0 30 106.0 31 103.0 32 121.0 33 118.0 34 223.0 35 525.0 36 2083.0 37 232.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.81909547738694 13.944723618090451 11.85929648241206 25.376884422110553 2 41.9 18.625 20.5 18.975 3 36.0 20.549999999999997 23.1 20.349999999999998 4 38.2 22.650000000000002 17.45 21.7 5 40.325 24.275 17.424999999999997 17.974999999999998 6 35.85 27.525 17.2 19.425 7 35.575 14.399999999999999 27.950000000000003 22.075 8 37.625 17.45 18.224999999999998 26.700000000000003 9 35.8 19.85 21.475 22.875 10-14 37.545 21.465 18.72 22.27 15-19 37.345 21.105 19.695 21.855 20-24 37.37934483620905 21.005251312828207 20.285071267816953 21.330332583145786 25-29 36.60915228807202 21.350337584396097 19.494873718429606 22.545636409102276 30-34 36.06901725431358 21.285321330332582 21.02525631407852 21.62040510127532 35-39 35.703925981495374 21.72043010752688 20.97024256064016 21.605401350337583 40-44 36.459114778694676 21.230307576894223 20.560140035008754 21.75043760940235 45-49 35.313828457114276 20.500125031257816 22.48562140535134 21.70042510627657 50-54 36.889222305576396 20.800200050012503 20.86521630407602 21.445361340335083 55-59 36.64916229057265 21.05026256564141 20.38009502375594 21.92048012003001 60-64 37.92948237059265 20.705176294073517 19.909977494373592 21.45536384096024 65-69 37.195579336900536 20.988148222233335 20.37305595839376 21.443216482472373 70-74 36.91922980745186 21.865466366591647 19.89497374343586 21.32033008252063 75-79 35.593898474618655 22.315578894723682 21.165291322830708 20.92523130782696 80-84 36.70417604401101 21.45036259064766 20.045011252813204 21.800450112528132 85-89 37.804451112778196 21.89047261815454 19.43985996499125 20.86521630407602 90-94 38.3295823955989 20.975243810952737 19.849962490622655 20.845211302825707 95-99 38.74468617154289 20.455113778444613 20.145036259064767 20.655163790947736 100-104 39.80995248812203 20.80520130032508 19.234808702175542 20.150037509377345 105-109 39.42485621405351 21.385346336584146 19.64491122780695 19.544886221555387 110-114 40.320080020005 20.46011502875719 19.05476369092273 20.16504126031508 115-119 40.700175043760936 20.615153788447113 19.70492623155789 18.97974493623406 120-124 42.195548887221804 20.655163790947736 18.234558639659916 18.91472868217054 125-129 42.365591397849464 21.030257564391096 18.37959489872468 18.22455613903476 130-134 43.39018656529785 20.122042714950233 18.216375731506027 18.271394988245888 135-139 44.633390017005105 19.710913273982193 18.270481144343304 17.3852155646694 140-144 45.191297824456115 19.64491122780695 17.99949987496874 17.164291072768194 145-149 46.651662915728934 19.344836209052264 17.239309827456864 16.76419104776194 150-151 47.76138069034517 18.82191095547774 16.45822911455728 16.95847923961981 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 2.0 11 2.0 12 0.5 13 1.0 14 1.0 15 1.0 16 0.5 17 1.5 18 1.5 19 0.0 20 0.5 21 1.0 22 1.0 23 1.0 24 0.5 25 1.0 26 3.0 27 4.5 28 3.5 29 2.0 30 4.0 31 3.0 32 2.5 33 6.0 34 11.5 35 26.0 36 30.5 37 28.0 38 41.5 39 69.5 40 94.0 41 94.5 42 110.0 43 114.5 44 106.5 45 137.5 46 142.5 47 137.5 48 133.0 49 142.5 50 154.0 51 133.0 52 119.5 53 110.0 54 109.5 55 103.5 56 94.0 57 81.0 58 73.0 59 78.0 60 69.0 61 62.5 62 65.5 63 69.0 64 77.0 65 69.0 66 51.0 67 54.0 68 63.0 69 61.0 70 48.5 71 35.5 72 35.0 73 32.0 74 30.5 75 22.5 76 13.5 77 16.0 78 12.5 79 10.5 80 10.5 81 5.5 82 3.0 83 4.5 84 5.0 85 4.5 86 5.5 87 6.5 88 5.5 89 6.0 90 5.0 91 10.5 92 16.5 93 13.5 94 14.5 95 13.0 96 11.5 97 23.5 98 47.0 99 88.5 100 169.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.015 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.034999999999999996 135-139 0.03 140-144 0.025 145-149 0.025 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.925 #Duplication Level Percentage of deduplicated Percentage of total 1 75.71908853193874 50.675000000000004 2 15.128875607022788 20.25 3 5.603287261860292 11.25 4 2.2786701531565186 6.1 5 0.7471049682480388 2.5 6 0.1867762420620097 0.75 7 0.03735524841240195 0.17500000000000002 8 0.0747104968248039 0.4 9 0.0 0.0 >10 0.1867762420620097 1.95 >50 0.0 0.0 >100 0.03735524841240195 5.949999999999999 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 238 5.949999999999999 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG 20 0.5 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG 19 0.475 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG 14 0.35000000000000003 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG 13 0.325 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG 12 0.3 No Hit TGGCCGCCCATGCTGCTCTCGCCGCGTCCCGCATCCCCACCGGCCGGCTG 8 0.2 No Hit AAACTCCCCTGACTGAAAAGAAAGAAACACCGCAGGAACGTCTTAAAAGG 8 0.2 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG 6 0.15 No Hit GGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTC 6 0.15 No Hit TGATGCACTACTGTTCTTCAGTCTCCACATTAACGCGACAACAGATCCAA 6 0.15 No Hit GCCGATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATCACGCGTA 6 0.15 No Hit CGTCAGTCGGCTTTGGCTCCGGTCTCTGCGCTCTCGTCTCCTTCCTGCTT 6 0.15 No Hit GCTCACTCATCACAGTCTCCTCCAGCAGCTAGCTCTTCTTCAACCCTTCA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG 5 0.125 No Hit CCAGTACATGCCGCTTGACAGCCAGAACAACGAGCACCAGCCTCTCAGGC 5 0.125 No Hit GTCTGTCCGGATGTTTGAAAAAGGAATTCAGACTAACCATGAACATCCTC 5 0.125 No Hit GTGATTTTGATAATTCAAATTTCCGGGAGCGACTCTGTGAAACTGTCGAG 5 0.125 No Hit CATTGTTGCCACTTGCCCTTTCATTTAAGGTGTCCAGTCATCTCGAGGAG 5 0.125 No Hit GAGCCGCGTCCGGCCGCCTCGAAGCTCCCTTCCCCACGGGCGGCGGGCTG 5 0.125 No Hit GAAAAGGCATCAGGTTTTGAGGAGTCCATGAAATACAAAAAGCTGACAAA 5 0.125 No Hit GTTGCTTGAGTCAGCTGCAAACATACACACCAGAATATGGTTTGCTTATC 5 0.125 No Hit AAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCAGC 5 0.125 No Hit GTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG 5 0.125 No Hit AACCAACCACATGATCGTTGATAAGATTTGGATCAGTGATGTATCCAAGT 5 0.125 No Hit GCGAGCACGGGGCGAAGAAGCAGGGGAAGCAGAGCAGGGTGTCTGACTCT 5 0.125 No Hit GCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAA 5 0.125 No Hit CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTA 5 0.125 No Hit TACATCTTCTGCCGACACTGGTTTTGCCGGAGTTCCTGGGTGTGATGAGC 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG 5 0.125 No Hit GAAGATTCCGCGGAAGGAGAGTTGGTGGAGGAGGGTTTGCTGCGTTGCGG 5 0.125 No Hit AAGATGTATCTACACATAAGCAGAACAAAAGGACACAACTATTTCATGGA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.0625 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.25 0.0 0.0 0.0 0.0 56-57 0.3625 0.0 0.0 0.0 0.0 58-59 0.4 0.0 0.0 0.0 0.0 60-61 0.425 0.0 0.0 0.0 0.0 62-63 0.48750000000000004 0.0 0.0 0.0 0.0 64-65 0.6375 0.0 0.0 0.0 0.0 66-67 0.7375 0.0 0.0 0.0 0.0 68-69 0.875 0.0 0.0 0.0 0.0 70-71 1.1124999999999998 0.0 0.0 0.0 0.0 72-73 1.3875000000000002 0.0 0.0 0.0 0.0 74-75 1.6375 0.0 0.0 0.0 0.0 76-77 1.8624999999999998 0.0 0.0 0.0 0.0 78-79 2.2 0.0 0.0 0.0 0.0 80-81 2.675 0.0 0.0 0.0 0.0 82-83 2.9875 0.0 0.0 0.0 0.0 84-85 3.3375 0.0 0.0 0.0 0.0 86-87 3.9749999999999996 0.0 0.0 0.0 0.0 88-89 4.875 0.0 0.0 0.0 0.0 90-91 5.262499999999999 0.0 0.0 0.0 0.0 92-93 5.775 0.0 0.0 0.0 0.0 94-95 6.15 0.0 0.0 0.0 0.0 96-97 6.7125 0.0 0.0 0.0 0.0 98-99 7.699999999999999 0.0 0.0 0.0 0.0 100-101 8.912500000000001 0.0 0.0 0.0 0.0 102-103 9.587499999999999 0.0 0.0 0.0 0.0 104-105 10.2875 0.0 0.0 0.0 0.0 106-107 10.7625 0.0 0.0 0.0 0.0 108-109 11.6375 0.0 0.0 0.0 0.0 110-111 12.5625 0.0 0.0 0.0 0.0 112-113 13.3625 0.0 0.0 0.0 0.0 114-115 14.05 0.0 0.0 0.0 0.0 116-117 14.825 0.0 0.0 0.0 0.0 118-119 15.899999999999999 0.0 0.0 0.0 0.0 120-121 16.9 0.0 0.0 0.0 0.0 122-123 17.5875 0.0 0.0 0.0 0.0 124-125 18.2 0.0 0.0 0.0 0.0 126-127 19.5625 0.0 0.0 0.0 0.0 128-129 20.5375 0.0 0.0 0.0 0.0 130-131 21.1875 0.0 0.0 0.0 0.0 132-133 22.0125 0.0 0.0 0.0 0.0 134-135 22.6375 0.0 0.0 0.0 0.0 136-137 23.625 0.0 0.0 0.0 0.0 138-139 24.725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTACA 10 0.006830828 145.0 6 GGAGGAT 10 0.006830828 145.0 1 >>END_MODULE Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221561 spots for SRR13165387.sra Written 1221561 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra Read 1221555 spots for SRR13165387.sra Written 1221555 spots for SRR13165387.sra SRR ids: ['SRR13165387.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_d3s9ck5m SRR13165387.sra spots: 24431106 blocks: [[1, 1221555], [1221556, 2443110], [2443111, 3664665], [3664666, 4886220], [4886221, 6107775], [6107776, 7329330], [7329331, 8550885], [8550886, 9772440], [9772441, 10993995], [10993996, 12215550], [12215551, 13437105], [13437106, 14658660], [14658661, 15880215], [15880216, 17101770], [17101771, 18323325], [18323326, 19544880], [19544881, 20766435], [20766436, 21987990], [21987991, 23209545], [23209546, 24431106]] SRR13165387 file size 8281058 SRR13165387 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165387 SRR13165387_1.fastq SRR13165387_2.fastq Input file: SRR13165387_1.fastq Paired file: SRR13165387_2.fastq trimmed: SRR13165387-trimmed-pair1.fastq, SRR13165387-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 17:21:04 2024 >> started Sat Dec 7 17:21:30 2024 >> done (26.219s) 24431106 read pairs processed; of these: 996 ( 0.00%) short read pairs filtered out after trimming by size control 2937857 (12.03%) empty read pairs filtered out after trimming by size control 21492253 (87.97%) read pairs available; of these: 6866683 (31.95%) trimmed read pairs available after processing 14625570 (68.05%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 35 0.00% 19 37 0.00% 20 38 0.00% 21 36 0.00% 22 61 0.00% 23 65 0.00% 24 93 0.00% 25 66 0.00% 26 134 0.00% 27 134 0.00% 28 203 0.00% 29 246 0.00% 30 392 0.00% 31 392 0.00% 32 434 0.00% 33 441 0.00% 34 569 0.00% 35 511 0.00% 36 712 0.00% 37 1197 0.01% 38 1734 0.01% 39 1222 0.01% 40 1796 0.01% 41 1705 0.01% 42 1671 0.01% 43 1823 0.01% 44 1674 0.01% 45 1978 0.01% 46 2194 0.01% 47 2342 0.01% 48 2934 0.01% 49 3376 0.02% 50 4063 0.02% 51 4625 0.02% 52 5333 0.02% 53 5172 0.02% 54 5546 0.03% 55 7072 0.03% 56 5998 0.03% 57 6806 0.03% 58 8665 0.04% 59 8570 0.04% 60 9839 0.05% 61 10734 0.05% 62 11984 0.06% 63 14614 0.07% 64 13250 0.06% 65 13671 0.06% 66 14245 0.07% 67 15501 0.07% 68 16780 0.08% 69 17889 0.08% 70 20950 0.10% 71 22899 0.11% 72 25870 0.12% 73 28469 0.13% 74 30313 0.14% 75 32002 0.15% 76 32145 0.15% 77 34403 0.16% 78 36474 0.17% 79 39763 0.19% 80 42361 0.20% 81 45706 0.21% 82 50289 0.23% 83 54188 0.25% 84 57364 0.27% 85 60049 0.28% 86 62888 0.29% 87 63578 0.30% 88 65350 0.30% 89 66261 0.31% 90 68650 0.32% 91 71996 0.33% 92 75911 0.35% 93 80287 0.37% 94 82517 0.38% 95 85951 0.40% 96 87722 0.41% 97 88287 0.41% 98 88375 0.41% 99 91029 0.42% 100 90580 0.42% 101 90080 0.42% 102 92193 0.43% 103 94408 0.44% 104 96460 0.45% 105 95473 0.44% 106 96348 0.45% 107 96264 0.45% 108 95537 0.44% 109 95108 0.44% 110 93897 0.44% 111 94914 0.44% 112 96419 0.45% 113 95991 0.45% 114 98602 0.46% 115 100090 0.47% 116 100202 0.47% 117 98269 0.46% 118 97870 0.46% 119 97622 0.45% 120 98816 0.46% 121 96785 0.45% 122 97935 0.46% 123 99342 0.46% 124 101290 0.47% 125 100842 0.47% 126 100648 0.47% 127 100886 0.47% 128 98017 0.46% 129 98857 0.46% 130 97567 0.45% 131 97697 0.45% 132 97406 0.45% 133 97912 0.46% 134 98101 0.46% 135 97790 0.46% 136 98524 0.46% 137 98244 0.46% 138 97946 0.46% 139 96816 0.45% 140 97209 0.45% 141 95196 0.44% 142 95901 0.45% 143 96259 0.45% 144 96759 0.45% 145 97360 0.45% 146 97056 0.45% 147 96231 0.45% 148 95259 0.44% 149 95218 0.44% 150 93838 0.44% 151 14625570 68.05% 21492253 reads passed initial QC criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=3.22 fanout-score-rank=35 prefix-density=1.82 prefix-fanout=1.0 sequence=CAGGTATCATCGCGTATGCCGTC criterion=fanout-score sequence-density=0.06 sequence-density-rank=31 fanout-score=57.30 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=10.4 sequence=CCAGCAGCAGTACCAGTCCTTAAGCAGAGGCCTTGTCAAACTTGAGGGGCTTCACCACCTCCCAGGTGAAGTCAGCATCATCACGGCCAAAGTGACCGTAGGCAGCGGTCTTGATGAACCTGTTGCCACCCTTCTTCAAGTCAAGGTTGATGCTGATCATACCAGGCCTGAAGTCAAAGTTCTCCTTCACGATCTTCAGAATCTCCTTGTCAGGGATGGTGCCGGTGCCGTAGGAGTCGACGAAGACAGACAAAGGCTCAGGGACACCAATGGCATAGGAGATCTGCACAATGCAGCGGCGTGCAAGACCACTGGCAATGATGCTTTTGGCGGCCTGCCTGGCAATGTAGGCACCACTGCGGTCAACCTTGGTTGGGTCCTTGCCAGAGAAGGCACCACCACCGTGGGCTCCCCAGCCACCGTAGGTGTCGATGATGATCTTGCGGCCGGTGAGACCAGCATCACCGTGAGGGCCACCGATGACAAAGCGGCCTGACGGGTTCAGGTGGAA criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=2.29 fanout-score-rank=36 prefix-density=0.20 prefix-fanout=2.2 sequence=TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGTGCCGGATTATGACTGAACGCCTCTAAGTCAGAATCCAAGCTAGCAAGCGGCGCCTGCGCCCGCCGCCTGCCCCGACCCACGTTAGGGGCGCTTGCGCCCCCAAGGGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCGCCTCGAAGCTCCCTTCCCCACGGGCGGCGGGCTGAATCCTTTGCAGACGACTTAAATACGCGACGGGGCATTGTAAGTGGCAGAGTGGCCTTGCTGCCACGATCCACTGAGATCCAGCCCCACGTCGCACGGA criterion=fanout-score sequence-density=0.06 sequence-density-rank=27 fanout-score=216.77 fanout-score-rank=1 prefix-density=0.63 prefix-fanout=21.4 sequence=CTGCTGCTGGATGTATCTCTGATTAATGAGTTGCTGCTCTTTAGAAGGAAGAAGGGGTTTGATATCGCCGCGGACACGCTGCATTGGCGTCTAGTGAGTGGTATTTTGGTGTGGCAGACAGAGTTACGTGCTGAGTTTATACTAGTCGGGTCTTTTGTTATCTTTTGTGGTTTTCCTTCGTTTTCGAGTCTAAAACTGCAATAGCTGTGCAGTTTGCTCTATCAGTCGTCC SRR13165387 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:22:24 Started mapping on | Dec 07 17:22:24 Finished on | Dec 07 17:24:58 Mapping speed, Million of reads per hour | 502.42 Number of input reads | 21492253 Average input read length | 278 UNIQUE READS: Uniquely mapped reads number | 17409958 Uniquely mapped reads % | 81.01% Average mapped length | 277.04 Number of splices: Total | 13674369 Number of splices: Annotated (sjdb) | 12750426 Number of splices: GT/AG | 13499848 Number of splices: GC/AG | 143248 Number of splices: AT/AC | 7951 Number of splices: Non-canonical | 23322 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.75 Insertion rate per base | 0.01% Insertion average length | 2.30 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 281146 % of reads mapped to multiple loci | 1.31% Number of reads mapped to too many loci | 554830 % of reads mapped to too many loci | 2.58% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.53% % of reads unmapped: other | 9.58% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3801389 3801389 3801389 N_multimapping 281146 281146 281146 N_noFeature 564566 16877472 784479 N_ambiguous 355403 2110 43401 UnstrandedReadsAssigned:16489989 PositiveStrandReadsAssigned:530376 NegativeStrandReadsAssigned:16582078 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=125 echo kmer=121 SRR13165387 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165387-trimmed-pair1.fastq SRR13165387-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,492,253 reads, 17,006,159 reads pseudoaligned [quant] estimated average fragment length: 205.038 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,186 rounds 52973 SRR13165387.ke.tsv 35125 SRR13165387.se.tsv 88098 total ==> SRR13165387.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 732.256 0 0 PNS24247 1044 839.962 55.2331 5.70396 PNS24249 1928 1723.96 123.683 6.22327 PNS24246 1044 839.962 55.2331 5.70396 PNS24248 1044 839.962 55.2331 5.70396 PNS24244 1471 1266.96 94.6179 6.47809 PNS24243 293 128.079 0 0 KQK14069 1603 1398.96 153.794 9.53612 KQK14071 474 280.914 0 0 ==> SRR13165387.se.tsv <== BRADI_1g14170v3 158 BRADI_1g53295v3 150 BRADI_1g59795v3 228 BRADI_1g07683v3 0 BRADI_1g00485v3 41 BRADI_1g20270v3 995 BRADI_1g74790v3 404 BRADI_1g09890v3 1 BRADI_1g77505v3 142 BRADI_1g48960v3 0 SRR13165387 completed mapping pipeline successfully