Starting /dee2/code/volunteer_pipeline.sh SRR13165388 current disk space = 1541308997632 free memory = 1602361492 SRR13165388 SRAfilesize 5c55433dfd0c9d9fe326ada272acfd7b SRR13165388.sra SRR13165388.sra file validated SRR13165388 is paired end SRR13165388 is conventional basespace SRR13165388 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165388_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5785 37.0 37.0 37.0 37.0 37.0 2 36.0505 37.0 37.0 37.0 37.0 37.0 3 36.4635 37.0 37.0 37.0 37.0 37.0 4 36.52 37.0 37.0 37.0 37.0 37.0 5 36.4485 37.0 37.0 37.0 37.0 37.0 6 36.5685 37.0 37.0 37.0 37.0 37.0 7 36.394 37.0 37.0 37.0 37.0 37.0 8 36.4865 37.0 37.0 37.0 37.0 37.0 9 36.5105 37.0 37.0 37.0 37.0 37.0 10-14 36.5421 37.0 37.0 37.0 37.0 37.0 15-19 36.4538 37.0 37.0 37.0 37.0 37.0 20-24 36.433800000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.397299999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.3628 37.0 37.0 37.0 37.0 37.0 35-39 36.3832 37.0 37.0 37.0 37.0 37.0 40-44 36.369600000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.2946 37.0 37.0 37.0 37.0 37.0 50-54 36.253 37.0 37.0 37.0 37.0 37.0 55-59 36.2803 37.0 37.0 37.0 37.0 37.0 60-64 36.227 37.0 37.0 37.0 37.0 37.0 65-69 36.1742 37.0 37.0 37.0 37.0 37.0 70-74 36.179199999999994 37.0 37.0 37.0 37.0 37.0 75-79 36.2195 37.0 37.0 37.0 37.0 37.0 80-84 36.1353 37.0 37.0 37.0 37.0 37.0 85-89 36.1518 37.0 37.0 37.0 37.0 37.0 90-94 36.0547 37.0 37.0 37.0 37.0 37.0 95-99 36.070899999999995 37.0 37.0 37.0 37.0 37.0 100-104 36.0273 37.0 37.0 37.0 37.0 37.0 105-109 36.0232 37.0 37.0 37.0 37.0 37.0 110-114 35.9829 37.0 37.0 37.0 37.0 37.0 115-119 36.0767 37.0 37.0 37.0 37.0 37.0 120-124 35.931599999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.9169 37.0 37.0 37.0 37.0 37.0 130-134 35.9436 37.0 37.0 37.0 37.0 37.0 135-139 35.843599999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.7945 37.0 37.0 37.0 37.0 37.0 145-149 35.547000000000004 37.0 37.0 37.0 37.0 37.0 150-151 35.37425 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 2.0 22 1.0 23 0.0 24 3.0 25 3.0 26 5.0 27 17.0 28 14.0 29 24.0 30 34.0 31 45.0 32 49.0 33 77.0 34 166.0 35 318.0 36 2847.0 37 394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.225 9.35 5.575 32.85 2 23.67758186397985 9.445843828715365 32.67002518891688 34.206549118387905 3 21.75 15.85 23.775 38.625 4 27.375 20.349999999999998 20.25 32.025 5 26.474999999999998 25.15 24.175 24.2 6 27.85 29.225 21.175 21.75 7 19.325 26.0 35.15 19.525000000000002 8 20.525 24.375 28.675 26.424999999999997 9 21.7 19.825 31.15 27.325 10-14 23.14 26.015 26.200000000000003 24.645 15-19 23.79 24.63 25.155 26.424999999999997 20-24 23.655 24.985 25.145 26.215 25-29 24.154999999999998 24.785 24.33 26.729999999999997 30-34 23.97 25.480000000000004 24.595 25.955000000000002 35-39 23.62 24.715 25.205 26.46 40-44 24.905 24.104999999999997 25.174999999999997 25.814999999999998 45-49 23.97 24.46 24.985 26.584999999999997 50-54 24.08 25.025 24.44 26.455000000000002 55-59 23.775 24.47 25.624999999999996 26.13 60-64 23.87 24.104999999999997 25.324999999999996 26.700000000000003 65-69 23.16 25.474999999999998 24.66 26.705000000000002 70-74 25.22 24.455 24.62 25.705 75-79 24.25 24.95 24.87 25.929999999999996 80-84 24.240000000000002 24.310000000000002 25.215 26.235000000000003 85-89 24.66 24.025 25.1 26.215 90-94 24.654999999999998 24.165 24.935 26.245 95-99 24.135 24.145 25.335 26.384999999999998 100-104 24.745 24.565 24.21 26.479999999999997 105-109 24.445 24.57 24.465 26.52 110-114 24.33 24.665 24.990000000000002 26.015 115-119 24.635 24.385 24.785 26.195 120-124 24.615000000000002 25.205 23.849999999999998 26.33 125-129 24.97 24.175 24.235 26.619999999999997 130-134 24.51 24.62 24.375 26.495 135-139 24.775 25.285000000000004 23.7 26.240000000000002 140-144 24.97 24.205 23.84 26.985 145-149 25.430000000000003 24.125 24.05 26.395000000000003 150-151 27.075 24.2 23.5 25.224999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 0.5 26 0.0 27 0.5 28 0.5 29 2.5 30 5.0 31 5.5 32 8.0 33 12.0 34 17.5 35 33.5 36 45.5 37 51.5 38 65.5 39 83.0 40 99.5 41 125.5 42 144.5 43 176.0 44 208.5 45 203.5 46 192.5 47 174.0 48 154.0 49 168.5 50 179.5 51 155.0 52 126.5 53 117.5 54 110.5 55 114.0 56 115.5 57 100.5 58 93.0 59 87.0 60 87.5 61 81.5 62 67.0 63 58.0 64 54.5 65 55.5 66 58.0 67 44.5 68 36.0 69 39.5 70 37.0 71 37.5 72 39.5 73 26.0 74 18.5 75 24.0 76 20.0 77 12.0 78 7.0 79 3.0 80 4.5 81 4.0 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.75 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.1 #Duplication Level Percentage of deduplicated Percentage of total 1 71.59913169319826 49.475 2 17.908827785817653 24.75 3 6.8379160636758325 14.174999999999999 4 2.279305354558611 6.3 5 0.723589001447178 2.5 6 0.5426917510853835 2.25 7 0.0723589001447178 0.35000000000000003 8 0.0361794500723589 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCGGCCAAACACCCGTATATACCTGTTGGGGCCTCATAATTTTATTGTC 8 0.2 No Hit GGCATTGACGCCGGCCGAACGAACTCGGAAGCAGAAGCAGCTTGCATCGA 7 0.17500000000000002 No Hit GTGGCGACCGCGGGGAAGACGGCGAGCCAGATGTAGCCCAGGGAAATGTC 7 0.17500000000000002 No Hit GTCTTTTCCAGAAGCATCATCTTTGTCATCTCTAGCATGTGAAGAACCTT 6 0.15 No Hit GGGGAAACAATGATTTCAGCACCACCCATGAAGTCATCCTGCAGATCCTT 6 0.15 No Hit CTGCTCCACAATTTCACGTACTTTACGGTTGTACTCCCGCTTGTTCTCGC 6 0.15 No Hit GCCACGGATCCTCCTAGCCAGCTGAATGTCCTTGGGCATGATAGTCACCC 6 0.15 No Hit GCCGTAGCCGTTTCCCTTCTTCACACACTGGGTCTTGTCAGCGCAGTCGC 6 0.15 No Hit GCAGCTCCTGAAGCACCCTCATCCATTGACATCGCCAGTGCCTGCTGTAG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTTAATCTCGTAT 6 0.15 TruSeq Adapter, Index 2 (97% over 39bp) GGCTGACGGGCTCCTTGGTCGCCGCCTTCACCGCGTCCACGGCGCCGCCC 6 0.15 No Hit GGCCGCCGAGGCTGAACCCGCCGCCGCCGTCATCGGCGGCCGGCTTCTTC 6 0.15 No Hit GCGCCTGCATTGCTGTAAATTTGATCAAATTCTGCAAAGTTTGGTGCTGG 6 0.15 No Hit GTTCATTTCACGAAGAAGCTGCAAGGCCATTAGATGATTGCATTGGCTAT 6 0.15 No Hit CTCGGCTTCAGGGTACATGTTGCAGCCGTTGCAGCCGCTGCCGCACTTGC 6 0.15 No Hit AGACAGAGGAAGGGAAAGATGGGATTCATATATATTCATAGCATCATGGA 6 0.15 No Hit CTCCTGGCCAACTCTGGTCTCCCCATTTTGATACAGGCCAGTGTAAACTG 6 0.15 No Hit CGGCAAGTTTCTTTCTTTGTTTCATGTGAGGCAATTGAACCCGTGTTGAC 6 0.15 No Hit AGCGCTTAGCCCATCCTTGCACTTCGCAGGAAGGAGCCGGTCCTGGTGAT 5 0.125 No Hit GTCTAATATTACTCTCCTGCTTTTAAAGTGGGCATCTTTAAGATTTTGCA 5 0.125 No Hit CGGGTTTTGTTGTCGATGACACTAAGCGGAGGCAAAGCCTTATAAGCGGA 5 0.125 No Hit GGGAGACACAAATAAATAGCAACAGAGATCGTCTTGTACAAAGTGCACAT 5 0.125 No Hit CTTGAAACTTCAGAATGGAATAATACCACATTGGCACCGGAAAACTTTTG 5 0.125 No Hit CCATAGTGCAGTTGCGGCATTACTGCTTCCACCACCAAGACCAGCACCAG 5 0.125 No Hit CACCTCTCCTCTCCGATCCAAAAACTCCCTAGCCCAACAAAAAATTGAAA 5 0.125 No Hit GCCATCAACAGTCGATTGGTAGAGGGTCTCCTCGAAGAGGATAGCACCAG 5 0.125 No Hit GTAACAGCAGCAGGAGTCTTAGGCTTGGCAGGTACATCCTCATCTGAGTC 5 0.125 No Hit GCCCAAGCTTCGCCGTCAGGTCATCCTTCATAGCTCCGACAACCCTGTCC 5 0.125 No Hit CGATAAATTTCATTCCATGCAAACACGTACGGACTCACATACAACCCGAA 5 0.125 No Hit GGCAGGTTTTCGACTACTGAAGACACCCAAACTCCTGGCTGTTGAAGTAG 5 0.125 No Hit CTGGAGTTGAGGCACCAGAAGTAACTCCTATTGTGATAGGCCCTTCTGGT 5 0.125 No Hit GGAGGAAGTAAGCCCAGAGGACGCCGGAGATGCCGCCGAAGAAGAACCCC 5 0.125 No Hit CCAAAGCCTGGCGGTAGAGTAATACGGGCTTCCTTCTTTGTCATAAGCCT 5 0.125 No Hit ACCATCTTGTCACCATAAAGATAAGCCGCAACTGCTCTCATCTAGTAATA 5 0.125 No Hit CATAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCGCGGGTCTGACACA 5 0.125 No Hit GCCCTAGCCTTACCTTACAATTCTTTGAGGAGTAAAATTGGACTCCCATA 5 0.125 No Hit GAAAAATCGTCCCGCCGCCACCTTCCCAGGGCGTGCCCGGCTTCGCCGGC 5 0.125 No Hit GTGGTTATCAATCCCAATGTAAGGCTACCAGTTGACATAGCTTTGGAGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0125 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.0625 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.0875 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.25 0.0 0.0 0.0 0.0 70-71 0.375 0.0 0.0 0.0 0.0 72-73 0.4 0.0 0.0 0.0 0.0 74-75 0.4125 0.0 0.0 0.0 0.0 76-77 0.4375 0.0 0.0 0.0 0.0 78-79 0.48750000000000004 0.0 0.0 0.0 0.0 80-81 0.525 0.0 0.0 0.0 0.0 82-83 0.575 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.825 0.0 0.0 0.0 0.0 88-89 0.9375 0.0 0.0 0.0 0.0 90-91 1.1749999999999998 0.0 0.0 0.0 0.0 92-93 1.3125 0.0 0.0 0.0 0.0 94-95 1.5625 0.0 0.0 0.0 0.0 96-97 1.95 0.0 0.0 0.0 0.0 98-99 2.125 0.0 0.0 0.0 0.0 100-101 2.525 0.0 0.0 0.0 0.0 102-103 2.9375 0.0 0.0 0.0 0.0 104-105 3.4 0.0 0.0 0.0 0.0 106-107 3.7 0.0 0.0 0.0 0.0 108-109 4.1 0.0 0.0 0.0 0.0 110-111 4.6 0.0 0.0 0.0 0.0 112-113 5.0875 0.0 0.0 0.0 0.0 114-115 5.525 0.0 0.0 0.0 0.0 116-117 6.0125 0.0 0.0 0.0 0.0 118-119 6.6 0.0 0.0 0.0 0.0 120-121 7.2125 0.0 0.0 0.0 0.0 122-123 7.862500000000001 0.0 0.0 0.0 0.0 124-125 8.2375 0.0 0.0 0.0 0.0 126-127 8.75 0.0 0.0 0.0 0.0 128-129 9.3125 0.0 0.0 0.0 0.0 130-131 9.8875 0.0 0.0 0.0 0.0 132-133 10.5 0.0 0.0 0.0 0.0 134-135 10.9375 0.0 0.0 0.0 0.0 136-137 11.6 0.0 0.0 0.0 0.0 138-139 12.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCACTA 10 0.006830828 145.0 5 >>END_MODULE SRR13165388 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165388_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0735 37.0 37.0 37.0 37.0 37.0 2 36.2975 37.0 37.0 37.0 37.0 37.0 3 36.322 37.0 37.0 37.0 37.0 37.0 4 36.246 37.0 37.0 37.0 37.0 37.0 5 36.3545 37.0 37.0 37.0 37.0 37.0 6 36.302 37.0 37.0 37.0 37.0 37.0 7 36.346 37.0 37.0 37.0 37.0 37.0 8 36.3745 37.0 37.0 37.0 37.0 37.0 9 36.3665 37.0 37.0 37.0 37.0 37.0 10-14 36.2479 37.0 37.0 37.0 37.0 37.0 15-19 36.1495 37.0 37.0 37.0 37.0 37.0 20-24 36.11 37.0 37.0 37.0 37.0 37.0 25-29 36.0477 37.0 37.0 37.0 37.0 37.0 30-34 36.01515 37.0 37.0 37.0 37.0 37.0 35-39 36.0166 37.0 37.0 37.0 37.0 37.0 40-44 35.9946 37.0 37.0 37.0 37.0 37.0 45-49 35.9988 37.0 37.0 37.0 37.0 37.0 50-54 35.9228 37.0 37.0 37.0 37.0 37.0 55-59 35.9427 37.0 37.0 37.0 37.0 37.0 60-64 35.898399999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.927749999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.876 37.0 37.0 37.0 37.0 37.0 75-79 35.8444 37.0 37.0 37.0 37.0 37.0 80-84 35.869749999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.7613 37.0 37.0 37.0 37.0 37.0 90-94 35.74335 37.0 37.0 37.0 37.0 37.0 95-99 35.7475 37.0 37.0 37.0 37.0 37.0 100-104 35.7774 37.0 37.0 37.0 37.0 37.0 105-109 35.6483 37.0 37.0 37.0 37.0 37.0 110-114 35.6243 37.0 37.0 37.0 37.0 37.0 115-119 35.497299999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.456900000000005 37.0 37.0 37.0 37.0 37.0 125-129 35.41015 37.0 37.0 37.0 37.0 37.0 130-134 35.245799999999996 37.0 37.0 37.0 34.6 37.0 135-139 35.0402 37.0 37.0 37.0 29.8 37.0 140-144 34.9028 37.0 37.0 37.0 25.0 37.0 145-149 34.7416 37.0 37.0 37.0 25.0 37.0 150-151 34.3605 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 4.0 13 4.0 14 16.0 15 7.0 16 0.0 17 3.0 18 3.0 19 1.0 20 2.0 21 6.0 22 6.0 23 2.0 24 5.0 25 7.0 26 13.0 27 8.0 28 11.0 29 20.0 30 24.0 31 53.0 32 65.0 33 96.0 34 220.0 35 473.0 36 2650.0 37 300.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.50402414486921 21.026156941649898 8.551307847082496 23.91851106639839 2 32.9 22.8 24.675 19.625 3 25.650000000000002 24.575 27.125 22.650000000000002 4 28.1 29.4 20.5 22.0 5 29.175 30.975 18.4 21.45 6 25.45 35.175 17.525 21.85 7 22.475 20.549999999999997 33.35 23.625 8 24.675 21.65 24.0 29.675 9 24.099999999999998 21.7 27.425 26.775 10-14 27.525 25.485000000000003 22.08 24.91 15-19 26.595000000000002 25.924999999999997 22.835 24.645 20-24 26.758027408222468 24.672401720516156 23.4370311093328 25.13253976192858 25-29 26.643321660830416 24.81240620310155 23.196598299149578 25.34767383691846 30-34 26.787054174378472 24.380971437146716 24.155870141563703 24.67610424691111 35-39 26.072821846553968 25.48264479343803 23.34700410123037 25.097529258777634 40-44 26.13045218087235 25.6702681072429 22.839135654261707 25.360144057623053 45-49 27.07082833133253 25.615246098439375 22.824129651860744 24.489795918367346 50-54 26.863058917675303 25.107532259677907 24.147244173251973 23.88216464939482 55-59 26.788394197098548 24.652326163081543 23.411705852926463 25.147573786893446 60-64 26.582974892467742 24.97249174752426 23.56707012103631 24.877463238971693 65-69 27.43911586738011 24.458668800320048 23.27349102365355 24.828724308646297 70-74 27.138569284642323 24.352176088044022 23.846923461730864 24.662331165582792 75-79 26.678339169584792 24.682341170585293 23.761880940470235 24.87743871935968 80-84 27.171792948237062 24.77119279819955 23.315828957239308 24.74118529632408 85-89 27.373686843421712 24.697348674337167 23.36168084042021 24.56728364182091 90-94 27.579652878507478 25.15380383134097 23.57825238833592 23.688290901815638 95-99 27.11813544063219 25.227568270481143 22.9518855656697 24.702410723216964 100-104 26.88844422211106 25.1775887943972 23.496748374187092 24.437218609304654 105-109 27.048524262131064 24.952476238119058 23.716858429214607 24.282141070535268 110-114 26.973091927578274 25.867760328098427 23.797139141742523 23.362008602580776 115-119 28.74937468734367 24.992496248124063 23.261630815407706 22.99649824912456 120-124 28.73936968484242 25.092546273136566 22.786393196598297 23.38169084542271 125-129 27.667450352658697 25.546495923165423 23.460557250762843 23.325496473413036 130-134 29.094547273636817 24.54727363681841 23.34667333666833 23.011505752876438 135-139 29.539769884942473 24.927463731865934 23.276638319159577 22.256128064032016 140-144 29.69890967290187 25.042512753826145 23.67710313093928 21.5814744423327 145-149 30.565282641320664 24.147073536768385 23.406703351675837 21.880940470235117 150-151 30.577788894447224 24.83741870935468 22.798899449724864 21.785892946473236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 0.5 13 0.5 14 0.5 15 0.5 16 1.0 17 0.5 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.5 24 1.0 25 1.5 26 1.0 27 0.0 28 1.5 29 1.5 30 3.0 31 4.5 32 4.5 33 6.5 34 14.0 35 25.5 36 30.5 37 41.0 38 59.0 39 79.5 40 110.0 41 127.0 42 148.5 43 172.0 44 175.5 45 182.5 46 186.5 47 163.5 48 136.5 49 166.5 50 184.5 51 160.5 52 137.5 53 129.0 54 132.5 55 112.0 56 89.0 57 94.0 58 107.0 59 101.5 60 78.0 61 67.0 62 66.5 63 60.0 64 56.0 65 54.0 66 60.0 67 60.5 68 55.5 69 41.5 70 41.0 71 50.5 72 38.0 73 35.5 74 31.5 75 17.5 76 18.5 77 16.5 78 10.5 79 7.0 80 1.5 81 1.0 82 2.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.5 88 1.0 89 1.0 90 1.0 91 2.0 92 1.0 93 0.5 94 1.5 95 1.5 96 0.5 97 1.0 98 2.0 99 1.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.045 35-39 0.03 40-44 0.04 45-49 0.04 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.015 70-74 0.05 75-79 0.05 80-84 0.025 85-89 0.05 90-94 0.034999999999999996 95-99 0.03 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.425 #Duplication Level Percentage of deduplicated Percentage of total 1 72.81238746849118 50.55 2 16.816708678429958 23.35 3 6.769895570759813 14.099999999999998 4 2.1606049693914295 6.0 5 0.8642419877565718 3.0 6 0.28808066258552395 1.2 7 0.10803024846957147 0.525 8 0.10803024846957147 0.6 9 0.0 0.0 >10 0.07202016564638099 0.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 14 0.35000000000000003 No Hit AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 13 0.325 No Hit TGGGATTCCCTACAGAATTTTTCCCTGTTCTGTTTGCAATTCCTCGCATG 8 0.2 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 8 0.2 No Hit CGGATCCGTTGCTAGGGATCGTCGCGGCGGCGTGCACTCTGTTCTGCGCC 8 0.2 No Hit GCTGCAATTGCAAGCTTGTGTCAAAGAAGAGGGTAGCACCTGATCCTCTT 7 0.17500000000000002 No Hit GAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAA 7 0.17500000000000002 No Hit GAACCCGGCTGTTTCCTTTGGCCCGGCGTTGGTTAGCTGGGAATGGGGGT 7 0.17500000000000002 No Hit CACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTT 6 0.15 No Hit GGGGATCCTGGACTTCATCGTGGGCGCCATCGTCAAGGACGAGCAGCTGC 6 0.15 No Hit GGACCTGATACGGGACACATTTCACCATTTCTTCAGAACCATAAGCCCAA 6 0.15 No Hit CTTAGATATCCTACAAAACCAGTGGAGCCCGATATATGATGTAGCTGCTA 6 0.15 No Hit CCAGCAAAGCGCAGTCAATAAAGGAACTTCCTGGAGCTTCAGTTAATGTT 6 0.15 No Hit GGTTCTTCTCTCTTGGCAATCTTTGGGCCATATCTTCATCTCTAACCACT 6 0.15 No Hit GTTCTCTGTGTCTCCTGTTGTGCGTGTTGCTGTTCAGTGCAAGGTGGCCT 6 0.15 No Hit CTGAAAAAAGGTCTCTCTACAGCATACACTAGGAAGAAGCAAGGAGATGG 6 0.15 No Hit GGACGCTCGGCCCGGCGATGGTCGCCGGCCGCTACAAAGGCATCTGGGTC 5 0.125 No Hit GCTGGGTTTTCATCAGAAACAGATCATAGCTCTGAGTGGTTAACCAAGTT 5 0.125 No Hit AACAAATGTTGCTGGTGTCCCAGTTGATGAAAGTAATTTGATCATCAAGG 5 0.125 No Hit TGATGAATCATCTGATGAGGAACCTGCAAAAAAGCCTGCTGCTAAACCTG 5 0.125 No Hit GAGCAATTCAGTGTTGAGGCCATGCCGACATTCCTATTTATGAAGGAAGG 5 0.125 No Hit GTTGGGGGAGCATTCCTTGCTAACTACCTATCTGAGAAGCTGGTTGGCCT 5 0.125 No Hit CACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTTCGAGC 5 0.125 No Hit GTCAGAATTGGGCCAAATAGAAACACTCAGTTGCTCATCGTGCCCTGTTC 5 0.125 No Hit CTCTTATTGAGGACCACCTTTGCGTCGAGCCAGCAAATGAGTACATTCAG 5 0.125 No Hit GTTCAATGACAACCAAAGGCCTTTGGAACAGCCTAACCGGCCATTGCCAA 5 0.125 No Hit GTACGGCGTGTCGTCCTTCGACGAGGGCGCGCCGTCCACCGCGCCCACGC 5 0.125 No Hit GCCGAAAAACAGAGCCCCCGCGTCGCCCTCCTCAGCGACCCGGGGGGACC 5 0.125 No Hit GAGTGAGGTTTAAGAGAGTGCTTGAATTGAATTTGATGATTTAGCTGTAA 5 0.125 No Hit ATCATCCATCAACCAAGGTTGATTAGTTAGAGATGTCTAGTTGCAAGGCT 5 0.125 No Hit GGACTGCGGGCTGAATCTGTTCTTCATCGTCTTCACATTAATGCTTGTGT 5 0.125 No Hit GGATGCCGGTGCCCTTCCACGGCGAGATGTTCGTCCTCGCCCGCGACGGC 5 0.125 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 5 0.125 No Hit GGCAGGAGCAGACGGGGCTACAGTTATTCCAAACGATGATTCAGGATGGC 5 0.125 No Hit GCCTCTTCTCGCTTGCTCTACCTGCTGCTTGCAACCATGGCACCCACCGT 5 0.125 No Hit CGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAAT 5 0.125 No Hit GCTCATCATCTTGTTTAATACCAAAGCTCTTCATATTCTCCTCCTTGATT 5 0.125 No Hit TGGATGTCTGTTTGGTTTGCTGTTCATTTGATCCTGAGATTATGACCGAT 5 0.125 No Hit GTCTAGTTGATGCTTCTCAAAACAGTTCTGACATCCTGCAGAGCACTATG 5 0.125 No Hit CTCCAGTAATACCTCTCATTTGCAAGAAATTGGAGAACTCAGTGGAATTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0125 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.0625 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.0875 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.25 0.0 0.0 0.0 0.0 70-71 0.375 0.0 0.0 0.0 0.0 72-73 0.4 0.0 0.0 0.0 0.0 74-75 0.4125 0.0 0.0 0.0 0.0 76-77 0.4375 0.0 0.0 0.0 0.0 78-79 0.48750000000000004 0.0 0.0 0.0 0.0 80-81 0.525 0.0 0.0 0.0 0.0 82-83 0.575 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.825 0.0 0.0 0.0 0.0 88-89 0.95 0.0 0.0 0.0 0.0 90-91 1.2000000000000002 0.0 0.0 0.0 0.0 92-93 1.3624999999999998 0.0 0.0 0.0 0.0 94-95 1.6124999999999998 0.0 0.0 0.0 0.0 96-97 2.025 0.0 0.0 0.0 0.0 98-99 2.2249999999999996 0.0 0.0 0.0 0.0 100-101 2.65 0.0 0.0 0.0 0.0 102-103 3.0250000000000004 0.0 0.0 0.0 0.0 104-105 3.425 0.0 0.0 0.0 0.0 106-107 3.6875 0.0 0.0 0.0 0.0 108-109 4.1 0.0 0.0 0.0 0.0 110-111 4.6 0.0 0.0 0.0 0.0 112-113 5.0875 0.0 0.0 0.0 0.0 114-115 5.550000000000001 0.0 0.0 0.0 0.0 116-117 6.0625 0.0 0.0 0.0 0.0 118-119 6.699999999999999 0.0 0.0 0.0 0.0 120-121 7.324999999999999 0.0 0.0 0.0 0.0 122-123 7.9625 0.0 0.0 0.0 0.0 124-125 8.350000000000001 0.0 0.0 0.0 0.0 126-127 8.875 0.0 0.0 0.0 0.0 128-129 9.4875 0.0 0.0 0.0 0.0 130-131 10.0125 0.0 0.0 0.0 0.0 132-133 10.625 0.0 0.0 0.0 0.0 134-135 11.0625 0.0 0.0 0.0 0.0 136-137 11.7 0.0 0.0 0.0 0.0 138-139 12.1625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCCCTT 10 0.006830828 145.0 6 >>END_MODULE Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641371 spots for SRR13165388.sra Written 1641371 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra Read 1641361 spots for SRR13165388.sra Written 1641361 spots for SRR13165388.sra SRR ids: ['SRR13165388.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_s255rjkd SRR13165388.sra spots: 32827230 blocks: [[1, 1641361], [1641362, 3282722], [3282723, 4924083], [4924084, 6565444], [6565445, 8206805], [8206806, 9848166], [9848167, 11489527], [11489528, 13130888], [13130889, 14772249], [14772250, 16413610], [16413611, 18054971], [18054972, 19696332], [19696333, 21337693], [21337694, 22979054], [22979055, 24620415], [24620416, 26261776], [26261777, 27903137], [27903138, 29544498], [29544499, 31185859], [31185860, 32827230]] SRR13165388 file size 11134428 SRR13165388 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165388 SRR13165388_1.fastq SRR13165388_2.fastq Input file: SRR13165388_1.fastq Paired file: SRR13165388_2.fastq trimmed: SRR13165388-trimmed-pair1.fastq, SRR13165388-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 17:21:07 2024 >> started Sat Dec 7 17:22:00 2024 >> done (53.380s) 32827230 read pairs processed; of these: 733 ( 0.00%) short read pairs filtered out after trimming by size control 111077 ( 0.34%) empty read pairs filtered out after trimming by size control 32715420 (99.66%) read pairs available; of these: 5796801 (17.72%) trimmed read pairs available after processing 26918619 (82.28%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 54 0.00% 19 62 0.00% 20 74 0.00% 21 92 0.00% 22 93 0.00% 23 113 0.00% 24 120 0.00% 25 174 0.00% 26 166 0.00% 27 213 0.00% 28 179 0.00% 29 220 0.00% 30 311 0.00% 31 265 0.00% 32 272 0.00% 33 272 0.00% 34 277 0.00% 35 308 0.00% 36 275 0.00% 37 324 0.00% 38 331 0.00% 39 394 0.00% 40 418 0.00% 41 507 0.00% 42 488 0.00% 43 449 0.00% 44 459 0.00% 45 489 0.00% 46 561 0.00% 47 588 0.00% 48 685 0.00% 49 873 0.00% 50 840 0.00% 51 985 0.00% 52 1094 0.00% 53 1082 0.00% 54 1253 0.00% 55 1233 0.00% 56 1453 0.00% 57 1599 0.00% 58 1787 0.01% 59 1975 0.01% 60 2110 0.01% 61 2374 0.01% 62 2706 0.01% 63 3019 0.01% 64 3240 0.01% 65 3487 0.01% 66 3757 0.01% 67 4202 0.01% 68 4700 0.01% 69 4967 0.02% 70 5508 0.02% 71 6187 0.02% 72 6888 0.02% 73 7915 0.02% 74 8618 0.03% 75 9422 0.03% 76 10725 0.03% 77 11376 0.03% 78 12285 0.04% 79 13517 0.04% 80 14357 0.04% 81 15635 0.05% 82 17513 0.05% 83 19156 0.06% 84 21948 0.07% 85 23520 0.07% 86 25799 0.08% 87 27172 0.08% 88 29090 0.09% 89 30049 0.09% 90 31630 0.10% 91 34280 0.10% 92 35994 0.11% 93 38296 0.12% 94 41335 0.13% 95 43823 0.13% 96 46643 0.14% 97 49389 0.15% 98 50817 0.16% 99 52342 0.16% 100 55132 0.17% 101 56522 0.17% 102 58230 0.18% 103 60409 0.18% 104 63173 0.19% 105 63631 0.19% 106 67203 0.21% 107 69796 0.21% 108 71035 0.22% 109 73696 0.23% 110 74835 0.23% 111 76035 0.23% 112 78820 0.24% 113 78894 0.24% 114 80709 0.25% 115 84063 0.26% 116 87322 0.27% 117 87019 0.27% 118 89912 0.27% 119 91236 0.28% 120 92631 0.28% 121 93976 0.29% 122 95287 0.29% 123 96915 0.30% 124 97932 0.30% 125 101678 0.31% 126 102198 0.31% 127 104612 0.32% 128 105236 0.32% 129 107939 0.33% 130 108136 0.33% 131 108984 0.33% 132 111040 0.34% 133 112087 0.34% 134 111604 0.34% 135 113966 0.35% 136 115119 0.35% 137 114524 0.35% 138 117124 0.36% 139 118294 0.36% 140 120726 0.37% 141 119819 0.37% 142 124026 0.38% 143 124874 0.38% 144 124584 0.38% 145 126974 0.39% 146 129922 0.40% 147 130667 0.40% 148 130835 0.40% 149 135346 0.41% 150 132876 0.41% 151 26918619 82.28% 32715420 reads passed initial QC criterion=sequence-density sequence-density=0.76 sequence-density-rank=1 fanout-score=2.28 fanout-score-rank=22 prefix-density=0.76 prefix-fanout=2.3 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=20.59 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=5.4 sequence=TTGCATCAATGATAATCCCAAAGTAGGAAGTGTGTACGTAGTAGCAAGTGTTATACATACATAAAATTAAGCGATGCGTTTCGATCAAGTACTTGGCATCATCGATCACATACGTACATTTCACAGCAGGTAGCTACGTACATTACAGGCATACGTACATGCAGAGAGATACCCGGCCCTGTGTTGTGTTTGCAATTGCATAGATGAGAATGAGATGACTTGATTCTTAATTAGCCGGCGAGAGCGCTGGTGTTGTAGACGAGGAGGGCGCCGCCGCCGAGGAGGCCGGCCAGGGTGACGGCCCAGATCAGGAGCCCCGTCGTCCCACCGGCGTAGCGGTCGCCAGATTCGGACCATACCTCCGGCGTGTAGATCGGGCTGTAGCCGTCGACGTTGGCGCCGTACTTGTCAACAAACTGGTACACACCCTTTCCCGTGCCCTTCCTGCCGTTGGCGTCGATGTCCACCGTCAAGCCACCTCCGATCCCGAGGGGCTTGTCGACCTTGATCT criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.91 fanout-score-rank=26 prefix-density=0.45 prefix-fanout=2.5 sequence=GGTGGTGCATGGC criterion=fanout-score sequence-density=0.13 sequence-density-rank=22 fanout-score=91.40 fanout-score-rank=1 prefix-density=0.73 prefix-fanout=15.6 sequence=GCCGCCGCCGCCA SRR13165388 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:22:43 Started mapping on | Dec 07 17:22:43 Finished on | Dec 07 17:26:11 Mapping speed, Million of reads per hour | 566.23 Number of input reads | 32715420 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 29731926 Uniquely mapped reads % | 90.88% Average mapped length | 291.00 Number of splices: Total | 29668326 Number of splices: Annotated (sjdb) | 27764675 Number of splices: GT/AG | 29246350 Number of splices: GC/AG | 364229 Number of splices: AT/AC | 13232 Number of splices: Non-canonical | 44515 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.59 Insertion rate per base | 0.01% Insertion average length | 2.46 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 805197 % of reads mapped to multiple loci | 2.46% Number of reads mapped to too many loci | 168814 % of reads mapped to too many loci | 0.52% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.76% % of reads unmapped: other | 2.38% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2178669 2178669 2178669 N_multimapping 805197 805197 805197 N_noFeature 1264199 28867779 1514408 N_ambiguous 734602 4767 121228 UnstrandedReadsAssigned:27733125 PositiveStrandReadsAssigned:859380 NegativeStrandReadsAssigned:28096290 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13165388 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165388-trimmed-pair1.fastq SRR13165388-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,715,420 reads, 28,712,549 reads pseudoaligned [quant] estimated average fragment length: 240.594 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,231 rounds 52973 SRR13165388.ke.tsv 35125 SRR13165388.se.tsv 88098 total ==> SRR13165388.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 696.906 0 0 PNS24247 1044 804.406 113.663 6.76317 PNS24249 1928 1688.41 233.419 6.61709 PNS24246 1044 804.406 113.663 6.76317 PNS24248 1044 804.406 113.663 6.76317 PNS24244 1471 1231.41 125.593 4.88172 PNS24243 293 107.17 0 0 KQK14069 1603 1363.41 704.228 24.7227 KQK14071 474 252.338 5.41161 1.02648 ==> SRR13165388.se.tsv <== BRADI_1g14170v3 730 BRADI_1g53295v3 175 BRADI_1g59795v3 792 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 542 BRADI_1g74790v3 533 BRADI_1g09890v3 1 BRADI_1g77505v3 544 BRADI_1g48960v3 4 SRR13165388 completed mapping pipeline successfully