Starting /dee2/code/volunteer_pipeline.sh SRR13172449 current disk space = 1551413411840 free memory = 1605448096 SRR13172449 SRAfilesize 6c02e7288a52396a744df59385ca8385 SRR13172449.sra SRR13172449.sra file validated SRR13172449 is paired end SRR13172449 is conventional basespace SRR13172449 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172449_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.7375 34.0 31.0 34.0 31.0 39.0 2 34.143 34.0 34.0 34.0 31.0 40.0 3 34.10175 34.0 34.0 34.0 31.0 40.0 4 36.92575 37.0 37.0 37.0 35.0 40.0 5 36.874 37.0 37.0 37.0 35.0 41.0 6 36.95325 37.0 37.0 37.0 35.0 41.0 7 36.86975 37.0 37.0 37.0 35.0 41.0 8 37.02225 37.0 37.0 37.0 35.0 41.0 9 38.60625 39.0 39.0 39.0 37.0 41.0 10-11 38.606125000000006 39.0 39.0 39.0 37.0 41.0 12-13 38.610625 39.0 39.0 39.0 37.0 41.0 14-15 39.366875 40.0 38.5 41.0 36.5 41.0 16-17 39.042375 40.0 38.0 41.0 35.0 41.0 18-19 39.04325 40.0 38.0 41.0 35.0 41.0 20-21 38.9135 40.0 38.0 41.0 35.0 41.0 22-23 38.780625 40.0 38.0 41.0 35.0 41.0 24-25 38.672 40.0 37.0 41.0 35.0 41.0 26-27 38.7115 40.0 37.0 41.0 35.0 41.0 28-29 38.671 40.0 37.0 41.0 35.0 41.0 30-31 38.49459304391978 40.0 36.5 41.0 35.0 41.0 32-33 38.53525981960757 40.0 37.5 41.0 35.0 41.0 34-35 38.52384682269401 40.0 38.0 41.0 35.0 41.0 36-37 38.50287406748707 40.0 37.0 41.0 35.0 41.0 38-39 38.355459217461515 40.0 37.0 41.0 34.5 41.0 40-41 38.36842769959446 40.0 37.0 41.0 35.0 41.0 42-43 38.15957258123025 40.0 37.0 41.0 34.0 41.0 44-45 38.026059085841695 40.0 36.0 41.0 34.0 41.0 46-47 38.05870320071027 40.0 36.0 41.0 33.5 41.0 48-49 37.89421007785941 40.0 35.0 41.0 33.0 41.0 50-51 37.81731429140977 39.0 35.0 41.0 33.5 41.0 52-53 37.56090250551078 39.0 35.0 41.0 33.0 41.0 54-55 37.51691805414268 39.0 35.0 41.0 33.0 41.0 56-57 37.264586258538614 39.0 35.0 41.0 33.0 41.0 58-59 37.22195546927283 38.5 35.0 40.5 33.0 41.0 60-61 36.905668413099534 38.0 35.0 40.0 33.0 41.0 62-63 36.537363868484235 37.0 35.0 40.0 32.0 41.0 64-65 36.27104473853813 36.5 35.0 40.0 32.0 41.0 66-67 36.10264936619659 36.0 35.0 39.0 32.0 41.0 68-69 35.88534561594217 36.0 35.0 39.0 32.0 41.0 70-71 35.44834960294662 35.0 34.5 38.5 31.0 40.0 72-73 35.10431984827146 35.0 34.0 37.0 31.0 40.0 74-75 34.45781552495817 35.0 33.5 37.0 30.0 39.0 76-77 33.46769628247354 34.5 32.0 35.5 28.5 38.5 78-79 34.223161356838276 35.0 33.5 36.0 30.5 37.5 80-81 34.32564558742882 35.0 34.0 36.0 31.0 37.0 82-83 34.12741492203985 35.0 34.0 35.0 31.0 37.0 84-85 33.96039406919695 35.0 34.0 35.0 30.5 36.5 86-87 33.81509845467687 35.0 34.0 35.0 31.0 36.0 88-89 33.77516018743874 35.0 34.0 35.0 31.0 36.0 90-91 33.58880408366274 35.0 33.5 35.0 30.5 35.5 92-93 33.42089920549809 35.0 33.0 35.0 29.5 35.0 94-95 33.234688482338434 35.0 33.0 35.0 29.0 35.0 96-97 33.39764743939332 35.0 33.0 35.0 30.0 35.0 98-99 33.0757431979262 34.5 33.0 35.0 29.0 35.0 100-101 33.059987446135935 34.5 32.0 35.0 28.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 0.0 25 6.0 26 9.0 27 13.0 28 22.0 29 30.0 30 33.0 31 52.0 32 95.0 33 115.0 34 225.0 35 441.0 36 724.0 37 1209.0 38 893.0 39 128.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.675 42.55 16.925 22.85 2 20.025000000000002 26.05 23.7 30.225 3 18.2 24.975 35.375 21.45 4 19.084542271135568 25.312656328164078 35.19259629814908 20.410205102551277 5 20.467689212974605 24.18908725169726 36.98767915514207 18.35554438018607 6 20.1 24.25 34.699999999999996 20.95 7 20.225 24.5 35.199999999999996 20.075000000000003 8 18.95 24.275 36.1 20.674999999999997 9 19.175 24.375 36.3 20.150000000000002 10-11 19.8 24.625 35.625 19.950000000000003 12-13 19.3625 24.8125 35.4125 20.4125 14-15 19.9125 24.15 35.525 20.4125 16-17 20.075000000000003 24.0625 35.6625 20.200000000000003 18-19 20.325 23.9875 36.05 19.6375 20-21 18.95 24.2875 35.9 20.8625 22-23 20.465058132266535 23.952994124265533 35.47943492936617 20.102512814101765 24-25 20.1125 24.0375 35.0125 20.837500000000002 26-27 19.400000000000002 24.2375 35.9125 20.45 28-29 19.8875 23.7 35.6 20.8125 30-31 19.51127346013352 24.096233782592265 34.89104421211739 21.50144854515682 32-33 19.82442348008386 26.10062893081761 33.700209643605874 20.37473794549266 34-35 22.08613728129206 25.34320323014805 31.17092866756393 21.399730820995963 36-37 22.655398037077425 26.33587786259542 30.070883315158127 20.93784078516903 38-39 20.896343139950506 26.766565850976082 29.87352213362662 22.463568875446796 40-41 22.01162147205313 27.476480354178197 28.624792473713338 21.88710570005534 42-43 22.4614529795805 27.253785247951107 28.65675788303931 21.62800388942909 44-45 20.968191964285715 27.469308035714285 28.836495535714285 22.726004464285715 46-47 21.68253079507279 27.36562150055991 28.947368421052634 22.00447928331467 48-49 22.505963238389224 27.809737617510876 27.374771993826297 22.30952715027361 50-51 22.6691042047532 26.986359161861905 28.645760090001403 21.69877654338349 52-53 22.270742358078603 27.80673334272433 27.73630088744894 22.18622341174813 54-55 22.258292166549047 26.93013408609739 28.49682427664079 22.314749470712776 56-57 22.02886247877759 28.041878890775322 27.886247877758912 22.043010752688172 58-59 22.85228239296853 28.21094414516586 27.473773745392684 21.462999716472922 60-61 22.201704545454547 27.201704545454547 28.735795454545453 21.860795454545453 62-63 22.5985484559556 28.774726056638677 26.668564109861958 21.958161377543757 64-65 22.144488863506567 27.869788692175902 27.027412906910335 22.958309537407196 66-67 22.199913953821884 29.284382618672023 26.186720206510827 22.32898322099527 68-69 21.16935483870968 29.349078341013822 26.569700460829495 22.911866359447007 70-71 21.48748372160324 30.27058312834611 26.334828534220804 21.907104615829837 72-73 22.028226393132545 29.375818419903972 26.553179106649203 22.042776080314272 74-75 22.15884159719175 29.910779581687873 26.707620301301745 21.222758519818637 76-77 22.357604227833235 29.330593071051087 26.086318261890778 22.225484439224896 78-79 22.0369267421084 29.92852888624181 25.77427039904705 22.26027397260274 80-81 22.432309786719102 29.965209499319318 24.761760701860535 22.84072001210104 82-83 22.734184914841848 31.00669099756691 24.224452554744524 22.034671532846716 84-85 22.29709035222052 29.173047473200615 25.803981623277185 22.72588055130168 86-87 22.882660917770252 29.84293193717277 24.91530643671081 22.359100708346165 88-89 22.861128332300062 30.90514569125852 23.853068815871048 22.380657160570365 90-91 22.62259125802914 30.549898167006113 24.753250822497257 22.074259752467494 92-93 21.785940468651045 29.480683977200762 25.12666244458518 23.606713109563017 94-95 21.902156420984873 30.543933054393307 24.589636305117477 22.964274219504343 96-97 23.113439188083156 30.31592732034703 23.800949418890163 22.769684072679652 98-99 22.3800768845061 30.06852749456794 24.92060839043958 22.63078723048638 100-101 22.366779540188915 30.885760114061668 24.345036535376938 22.402423810372483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 84.0 1 178.5 2 357.0 3 415.5 4 330.5 5 140.5 6 7.5 7 4.0 8 3.0 9 3.0 10 2.0 11 2.0 12 3.5 13 3.0 14 3.0 15 5.5 16 9.0 17 11.0 18 9.5 19 8.5 20 8.5 21 7.5 22 8.5 23 10.5 24 12.0 25 15.0 26 16.5 27 18.0 28 20.0 29 15.5 30 20.5 31 28.0 32 29.0 33 40.5 34 57.0 35 65.5 36 69.5 37 82.5 38 99.0 39 120.5 40 130.0 41 135.5 42 145.5 43 136.0 44 132.5 45 138.5 46 140.0 47 135.5 48 136.0 49 128.5 50 108.5 51 97.0 52 95.0 53 88.0 54 76.5 55 71.5 56 64.5 57 55.5 58 47.0 59 43.5 60 46.5 61 49.0 62 45.5 63 44.0 64 37.0 65 36.0 66 39.0 67 32.0 68 32.0 69 27.5 70 24.5 71 26.0 72 24.5 73 22.0 74 15.0 75 10.5 76 9.5 77 8.0 78 6.0 79 4.0 80 2.5 81 3.5 82 2.5 83 0.5 84 0.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.05 5 0.575 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0125 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.013948946854512484 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 144.0 32-33 126.0 34-35 51.0 36-37 35.0 38-39 25.0 40-41 15.0 42-43 16.0 44-45 12.0 46-47 11.0 48-49 9.0 50-51 5.0 52-53 4.0 54-55 11.0 56-57 7.0 58-59 8.0 60-61 6.0 62-63 7.0 64-65 17.0 66-67 15.0 68-69 16.0 70-71 19.0 72-73 17.0 74-75 16.0 76-77 10.0 78-79 91.0 80-81 13.0 82-83 25.0 84-85 16.0 86-87 20.0 88-89 31.0 90-91 37.0 92-93 46.0 94-95 47.0 96-97 70.0 98-99 95.0 100-101 2907.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.425 #Duplication Level Percentage of deduplicated Percentage of total 1 98.8140005785363 85.39999999999999 2 0.26034133641886026 0.44999999999999996 3 0.26034133641886026 0.675 4 0.08678044547295344 0.3 5 0.11570726063060456 0.5 6 0.11570726063060456 0.6 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.20248770610355799 3.5749999999999997 >50 0.1446340757882557 8.5 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 2.0500000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 1.975 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65 1.625 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 1.5 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 1.35 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 1.075 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.7250000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.44999999999999996 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTTT 75 0.0 72.1825 2 ACTTTTT 80 0.0 67.6711 1 >>END_MODULE SRR13172449 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172449_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.1975 34.0 31.0 34.0 31.0 41.0 2 34.45625 34.0 33.0 34.0 31.0 41.0 3 34.42825 34.0 33.0 34.0 31.0 41.0 4 37.18975 37.0 37.0 37.0 35.0 41.0 5 37.1345 37.0 37.0 37.0 35.0 41.0 6 37.26475 37.0 37.0 37.0 35.0 41.0 7 37.2275 37.0 37.0 37.0 35.0 41.0 8 37.08125 37.0 37.0 37.0 35.0 41.0 9 38.39975 39.0 39.0 39.0 37.0 41.0 10-11 38.417 39.0 39.0 39.0 37.0 41.0 12-13 38.467375000000004 39.0 39.0 39.0 37.0 41.0 14-15 38.863125 40.0 38.0 41.0 36.0 41.0 16-17 38.539375 40.0 37.0 41.0 35.0 41.0 18-19 38.476749999999996 40.0 36.5 41.0 35.0 41.0 20-21 38.542375 40.0 36.5 41.0 35.0 41.0 22-23 38.298249999999996 40.0 36.0 41.0 35.0 41.0 24-25 38.075125 40.0 35.0 41.0 34.5 41.0 26-27 38.2285 40.0 35.0 41.0 35.0 41.0 28-29 38.173 40.0 35.0 41.0 34.0 41.0 30-31 38.167574548001014 40.0 35.5 41.0 34.5 41.0 32-33 38.20146263996161 40.0 36.0 41.0 34.0 41.0 34-35 38.15386606819308 40.0 37.0 41.0 34.0 41.0 36-37 37.91409385871336 40.0 36.0 41.0 34.0 41.0 38-39 37.9904000073542 40.0 36.0 41.0 34.0 41.0 40-41 37.724901978793454 39.5 35.0 41.0 33.0 41.0 42-43 37.488116371034664 39.0 35.0 41.0 33.0 41.0 44-45 37.450558658343155 39.0 35.0 41.0 33.0 41.0 46-47 37.0578502225937 38.5 35.0 40.5 32.0 41.0 48-49 37.22583881010031 39.0 35.0 40.5 33.0 41.0 50-51 36.643019907022136 38.0 35.0 40.0 32.0 40.5 52-53 36.93210494477618 38.0 35.0 40.0 33.0 40.5 54-55 37.256905584822434 39.0 35.0 41.0 33.0 41.0 56-57 37.21543653006656 39.0 35.0 41.0 33.0 41.0 58-59 36.98649387472135 38.0 35.0 40.5 33.0 41.0 60-61 36.777273335474206 37.0 35.0 40.5 32.5 41.0 62-63 36.45981309166754 37.0 35.0 40.0 32.5 41.0 64-65 36.247520518500465 36.0 35.0 40.0 32.0 41.0 66-67 36.03870450391838 36.0 35.0 39.0 32.0 41.0 68-69 35.59354800770528 35.0 35.0 39.0 31.0 41.0 70-71 35.32589656460897 35.0 34.0 38.5 31.0 40.0 72-73 35.1282697815426 35.0 34.0 37.0 31.0 40.0 74-75 34.845652504404285 35.0 34.0 37.0 31.0 39.0 76-77 34.662979211584826 35.0 34.0 36.0 31.0 39.0 78-79 34.267563242285185 35.0 34.0 36.0 30.5 37.5 80-81 34.07715546508822 35.0 34.0 36.0 30.0 37.0 82-83 33.92325542157702 35.0 34.0 35.0 30.0 37.0 84-85 33.733388771024565 35.0 33.5 35.0 30.0 36.0 86-87 33.50912893985287 35.0 33.5 35.0 29.5 36.0 88-89 33.38432178407729 35.0 33.0 35.0 29.0 36.0 90-91 33.30735960268854 35.0 33.0 35.0 29.5 35.5 92-93 33.21530782148166 35.0 33.0 35.0 29.0 35.0 94-95 33.35893048103288 35.0 33.0 35.0 30.0 35.0 96-97 33.262895026603445 35.0 33.0 35.0 30.0 35.0 98-99 33.320202161376166 35.0 33.0 35.0 29.5 35.0 100-101 32.86172717026466 34.5 32.0 35.0 28.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 4.0 23 3.0 24 7.0 25 16.0 26 11.0 27 13.0 28 21.0 29 33.0 30 49.0 31 65.0 32 98.0 33 143.0 34 223.0 35 450.0 36 730.0 37 1201.0 38 851.0 39 81.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.225 46.975 15.975 20.825 2 19.5 22.45 21.125 36.925000000000004 3 16.725 27.925 35.55 19.8 4 18.375 21.075 42.5 18.05 5 25.55 22.275 35.25 16.925 6 25.825 20.849999999999998 35.325 18.0 7 25.775 21.9 34.849999999999994 17.474999999999998 8 20.3 22.95 38.25 18.5 9 20.175 23.425 37.6 18.8 10-11 20.424999999999997 23.1875 36.6875 19.7 12-13 20.0625 22.900000000000002 37.625 19.412499999999998 14-15 19.975 23.1 37.225 19.7 16-17 20.3375 23.2625 36.3875 20.0125 18-19 20.7375 21.95 37.5 19.8125 20-21 19.475 22.4375 37.7875 20.3 22-23 20.200000000000003 23.0625 37.1875 19.55 24-25 19.900000000000002 23.225 37.65 19.225 26-27 19.7375 23.125 36.8625 20.275000000000002 28-29 19.725 23.625 36.7125 19.9375 30-31 20.058029519364197 23.716412261889744 35.92784155418191 20.29771666456415 32-33 21.458718016354524 24.782379319440782 33.9356370350831 19.823265629121604 34-35 21.49176534639989 25.792840615217095 30.883353749829862 21.832040288553152 36-37 22.257354962881497 25.14434973879571 30.79461094308496 21.803684355237834 38-39 22.11458621644063 25.17298643786327 31.428176030999172 21.284251314696927 40-41 22.683706070287542 24.600638977635782 30.990415335463258 21.72523961661342 42-43 22.644927536231883 26.226309921962095 30.04459308807135 21.08416945373467 44-45 22.63570229434807 25.461667599328486 30.442081701175155 21.460548405148295 46-47 22.445537596626846 27.43499648629656 28.657765284609976 21.46170063246662 48-49 22.835314091680814 25.82059988681381 29.654782116581778 21.689303904923598 50-51 22.212753870188894 26.51611986933674 29.34242295128533 21.928703309189036 52-53 22.597661819218708 27.074422583404615 28.414599372683202 21.91331622469347 54-55 21.66881351080578 27.035923858594536 29.168455703449265 22.126806927150422 56-57 22.733792312105567 26.764199655765918 28.55708548479633 21.944922547332187 58-59 21.855640397637227 27.820198818614035 29.00158478605388 21.322575997694855 60-61 22.310584152689415 27.154424522845577 28.51359167148641 22.0213996529786 62-63 22.257690075449794 27.582704585026118 28.32269297736506 21.836912362159026 64-65 22.304995617879054 27.78264680105171 27.7972538708735 22.115103710195736 66-67 22.777369581190303 27.450404114621602 27.494489346069066 22.27773695811903 68-69 23.25615690901047 27.414835569974933 27.562306444477215 21.766701076537384 70-71 21.664445431660003 28.7575892196061 27.17310824818599 22.404857100547904 72-73 23.470754576573896 27.504092870962943 27.1617800267897 21.863372525673462 74-75 22.024521531100476 28.797846889952154 27.57177033492823 21.605861244019138 76-77 23.05145952452603 28.573578092085466 26.752934095696663 21.622028287691844 78-79 23.017902813299234 29.395780051150894 25.31969309462916 22.266624040920714 80-81 21.867572156196943 30.1018675721562 25.314091680814943 22.716468590831916 82-83 22.467110883307704 29.813770715872202 26.29420809841107 21.42491030240902 84-85 24.212700051626225 28.72139046635691 25.05592841163311 22.009981070383756 86-87 22.118218062055817 30.438550875368346 25.56768937424164 21.8755416883342 88-89 23.704481792717086 29.429271708683473 24.317226890756302 22.54901960784314 90-91 23.770058190795275 29.14829836007759 24.722271204373126 22.35937224475401 92-93 22.51113089937667 30.186999109528053 24.951024042742652 22.350845948352628 94-95 22.49278499278499 30.266955266955264 25.054112554112557 22.18614718614719 96-97 23.318632855567806 28.776185226019845 24.45791988239618 23.44726203601617 98-99 22.76911516062371 29.795228254743567 24.83561901183543 22.600037572797294 100-101 22.399193548387096 30.967741935483872 24.778225806451612 21.85483870967742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 58.0 1 187.5 2 394.5 3 434.0 4 350.5 5 155.0 6 4.5 7 3.5 8 2.0 9 2.0 10 3.5 11 2.5 12 1.5 13 4.0 14 5.0 15 5.0 16 5.5 17 5.5 18 6.5 19 8.0 20 8.0 21 7.0 22 9.0 23 9.0 24 8.5 25 11.0 26 15.5 27 20.0 28 19.5 29 17.5 30 19.0 31 23.0 32 33.0 33 44.5 34 52.5 35 57.5 36 61.5 37 74.5 38 98.0 39 114.0 40 120.5 41 132.5 42 142.0 43 151.0 44 161.0 45 153.5 46 139.5 47 136.5 48 127.5 49 115.0 50 111.0 51 101.0 52 90.0 53 86.5 54 85.5 55 79.0 56 72.5 57 75.0 58 61.0 59 49.0 60 45.0 61 41.0 62 48.5 63 48.0 64 39.0 65 37.0 66 36.0 67 28.0 68 25.5 69 30.5 70 32.0 71 29.0 72 25.0 73 21.0 74 17.0 75 12.5 76 11.5 77 10.5 78 9.0 79 7.0 80 5.5 81 4.0 82 2.0 83 2.0 84 2.5 85 3.0 86 3.5 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 163.0 32-33 147.0 34-35 47.0 36-37 27.0 38-39 12.0 40-41 11.0 42-43 15.0 44-45 17.0 46-47 21.0 48-49 18.0 50-51 12.0 52-53 16.0 54-55 5.0 56-57 14.0 58-59 15.0 60-61 12.0 62-63 17.0 64-65 24.0 66-67 15.0 68-69 12.0 70-71 17.0 72-73 13.0 74-75 22.0 76-77 39.0 78-79 338.0 80-81 21.0 82-83 20.0 84-85 19.0 86-87 28.0 88-89 23.0 90-91 28.0 92-93 31.0 94-95 43.0 96-97 65.0 98-99 94.0 100-101 2579.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.1 #Duplication Level Percentage of deduplicated Percentage of total 1 98.50176263219741 83.825 2 0.49941245593419503 0.8500000000000001 3 0.17626321974148063 0.44999999999999996 4 0.11750881316098707 0.4 5 0.14688601645123384 0.625 6 0.08813160987074031 0.44999999999999996 7 0.11750881316098707 0.7000000000000001 8 0.05875440658049354 0.4 9 0.0 0.0 >10 0.14688601645123384 2.55 >50 0.14688601645123384 9.75 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 91 2.275 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 2.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 2.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 1.775 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 1.325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.9249999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 0.775 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0125 0.0 0.0 72-73 0.0 0.0 0.025 0.0 0.0 74-75 0.0 0.0 0.025 0.0 0.0 76-77 0.0 0.0 0.025 0.0 0.0 78-79 0.0 0.0 0.025 0.0 0.0 80-81 0.0 0.0 0.025 0.0 0.0 82-83 0.0 0.0 0.025 0.0 0.0 84-85 0.0 0.0 0.025 0.0 0.0 86-87 0.0 0.0 0.025 0.0 0.0 88-89 0.0 0.0 0.025 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 50 0.0 81.5875 1 CATGGGG 15 0.0011246304 81.5875 2 CATGGGA 20 3.2768512E-5 81.5875 2 CTTTTTT 50 0.0 81.5875 2 ACATGGG 55 0.0 74.170456 1 >>END_MODULE Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568860 spots for SRR13172449.sra Written 1568860 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra Read 1568845 spots for SRR13172449.sra Written 1568845 spots for SRR13172449.sra SRR ids: ['SRR13172449.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_utvozuw6 SRR13172449.sra spots: 31376915 blocks: [[1, 1568845], [1568846, 3137690], [3137691, 4706535], [4706536, 6275380], [6275381, 7844225], [7844226, 9413070], [9413071, 10981915], [10981916, 12550760], [12550761, 14119605], [14119606, 15688450], [15688451, 17257295], [17257296, 18826140], [18826141, 20394985], [20394986, 21963830], [21963831, 23532675], [23532676, 25101520], [25101521, 26670365], [26670366, 28239210], [28239211, 29808055], [29808056, 31376915]] SRR13172449 file size 6879270 SRR13172449 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172449 SRR13172449_1.fastq SRR13172449_2.fastq Input file: SRR13172449_1.fastq Paired file: SRR13172449_2.fastq trimmed: SRR13172449-trimmed-pair1.fastq, SRR13172449-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:46:51 2024 >> started Fri Dec 6 10:47:17 2024 >> done (26.936s) 31376915 read pairs processed; of these: 2 ( 0.00%) short read pairs filtered out after trimming by size control 40 ( 0.00%) empty read pairs filtered out after trimming by size control 31376873 (100.00%) read pairs available; of these: 1255105 ( 4.00%) trimmed read pairs available after processing 30121768 (96.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 0 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 4 0.00% 28 10 0.00% 29 571 0.00% 30 7099 0.02% 31 12357 0.04% 32 14857 0.05% 33 14238 0.05% 34 14296 0.05% 35 13929 0.04% 36 14350 0.05% 37 14997 0.05% 38 16210 0.05% 39 17152 0.05% 40 18143 0.06% 41 19474 0.06% 42 21169 0.07% 43 22374 0.07% 44 25252 0.08% 45 28279 0.09% 46 30696 0.10% 47 34309 0.11% 48 38522 0.12% 49 43026 0.14% 50 48253 0.15% 51 54323 0.17% 52 59761 0.19% 53 65436 0.21% 54 78790 0.25% 55 85482 0.27% 56 90502 0.29% 57 95988 0.31% 58 104127 0.33% 59 112616 0.36% 60 123156 0.39% 61 133604 0.43% 62 155279 0.49% 63 198436 0.63% 64 262542 0.84% 65 1139139 3.63% 66 1860180 5.93% 67 985499 3.14% 68 443241 1.41% 69 298600 0.95% 70 258493 0.82% 71 245047 0.78% 72 231377 0.74% 73 222619 0.71% 74 245082 0.78% 75 210500 0.67% 76 202790 0.65% 77 203648 0.65% 78 253431 0.81% 79 252284 0.80% 80 241032 0.77% 81 222648 0.71% 82 249444 0.79% 83 260834 0.83% 84 265807 0.85% 85 266490 0.85% 86 295937 0.94% 87 305798 0.97% 88 419380 1.34% 89 2947810 9.39% 90 183180 0.58% 91 139869 0.45% 92 120266 0.38% 93 129764 0.41% 94 151232 0.48% 95 182636 0.58% 96 227396 0.72% 97 340343 1.08% 98 783569 2.50% 99 1034309 3.30% 100 3335715 10.63% 101 10131874 32.29% 31376873 reads passed initial QC criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=4.13 fanout-score-rank=30 prefix-density=0.24 prefix-fanout=3.4 sequence=AGCTAGCTAGCT criterion=fanout-score sequence-density=0.05 sequence-density-rank=11 fanout-score=137.10 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=18.2 sequence=CTGCTGCTGCTG criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=3.93 fanout-score-rank=30 prefix-density=0.22 prefix-fanout=3.3 sequence=AGCTAGCTAGCT criterion=fanout-score sequence-density=0.05 sequence-density-rank=12 fanout-score=200.27 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=24.7 sequence=AAGAAGAAGAAA SRR13172449 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:48:01 Started mapping on | Dec 06 10:48:02 Finished on | Dec 06 10:55:03 Mapping speed, Million of reads per hour | 268.31 Number of input reads | 31376873 Average input read length | 175 UNIQUE READS: Uniquely mapped reads number | 22430736 Uniquely mapped reads % | 71.49% Average mapped length | 178.89 Number of splices: Total | 7804286 Number of splices: Annotated (sjdb) | 7198880 Number of splices: GT/AG | 7621892 Number of splices: GC/AG | 95312 Number of splices: AT/AC | 5264 Number of splices: Non-canonical | 81818 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 1.43 Insertion rate per base | 0.00% Insertion average length | 1.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1977286 % of reads mapped to multiple loci | 6.30% Number of reads mapped to too many loci | 158395 % of reads mapped to too many loci | 0.50% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 21.42% % of reads unmapped: other | 0.29% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10232427 10232427 10232427 N_multimapping 1977286 1977286 1977286 N_noFeature 774599 11928343 10780644 N_ambiguous 595396 50827 55886 UnstrandedReadsAssigned:21060741 PositiveStrandReadsAssigned:10451566 NegativeStrandReadsAssigned:11594206 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=88 echo kmer=83 SRR13172449 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172449-trimmed-pair1.fastq SRR13172449-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,376,873 reads, 25,791,338 reads pseudoaligned [quant] estimated average fragment length: 155.036 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,177 rounds 52973 SRR13172449.ke.tsv 35125 SRR13172449.se.tsv 88098 total ==> SRR13172449.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 782.005 0 0 PNS24247 1044 889.964 0 0 PNS24249 1928 1773.96 0 0 PNS24246 1044 889.964 0 0 PNS24248 1044 889.964 0 0 PNS24244 1471 1316.96 353 14.8834 PNS24243 293 141.906 0 0 KQK14069 1603 1448.96 135 5.1734 KQK14071 474 320.895 0 0 ==> SRR13172449.se.tsv <== BRADI_1g14170v3 129 BRADI_1g53295v3 0 BRADI_1g59795v3 125 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 1047 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 215 BRADI_1g48960v3 0 SRR13172449 completed mapping pipeline successfully