Starting /dee2/code/volunteer_pipeline.sh SRR13172450 current disk space = 1551780544512 free memory = 1604073836 SRR13172450 SRAfilesize c5e274b08b3b12efe852cafdccea63a9 SRR13172450.sra SRR13172450.sra file validated SRR13172450 is paired end SRR13172450 is conventional basespace SRR13172450 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172450_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 13 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.36125 34.0 34.0 37.0 31.0 40.0 2 34.988 34.0 34.0 39.0 31.0 41.0 3 34.92575 34.0 34.0 38.0 31.0 41.0 4 37.18325 37.0 37.0 38.0 35.0 41.0 5 37.00725 37.0 37.0 38.0 35.0 41.0 6 37.1595 37.0 37.0 39.0 35.0 41.0 7 37.20275 37.0 37.0 39.0 35.0 41.0 8 37.06925 37.0 37.0 39.0 35.0 41.0 9 38.2 39.0 38.0 39.0 35.0 41.0 10-11 38.162 39.0 39.0 39.0 35.0 41.0 12-13 38.09025 39.0 38.0 39.0 35.0 41.0 14-15 38.050375 40.0 37.0 41.0 34.0 41.0 16-17 36.933499999999995 38.5 35.0 40.0 33.0 41.0 18-19 35.611875 36.0 35.0 38.5 33.0 41.0 20-21 35.12025 35.0 35.0 38.0 33.0 41.0 22-23 34.9225 35.0 35.0 37.0 32.0 41.0 24-25 34.96375 35.0 35.0 36.5 32.5 41.0 26-27 34.843625 35.0 35.0 35.5 32.0 41.0 28-29 34.749624999999995 35.0 35.0 35.0 31.0 41.0 30-31 34.71903236040609 35.0 35.0 35.0 31.0 41.0 32-33 34.75860336029828 35.0 35.0 35.0 31.0 41.0 34-35 34.84023879064158 35.0 35.0 36.0 32.0 41.0 36-37 34.729694813028615 35.0 35.0 35.5 31.0 41.0 38-39 34.695478146906545 35.0 35.0 35.5 31.0 41.0 40-41 34.665924268748654 35.0 35.0 36.0 31.0 41.0 42-43 34.53313637984154 35.0 35.0 35.0 31.0 41.0 44-45 34.61740682676914 35.0 35.0 35.0 31.0 41.0 46-47 34.50764300782596 35.0 35.0 35.0 31.0 41.0 48-49 34.592005051949286 35.0 35.0 35.0 31.0 40.0 50-51 34.66857809238972 35.0 35.0 35.0 31.0 40.0 52-53 34.528262355210686 35.0 35.0 35.0 30.5 40.0 54-55 34.462431634927256 35.0 34.5 35.0 30.5 40.0 56-57 34.60435584078651 35.0 35.0 35.0 31.0 40.0 58-59 34.81541839062636 35.0 35.0 35.0 33.0 40.0 60-61 34.55565573260582 35.0 35.0 35.0 31.0 40.0 62-63 34.53654525988142 35.0 35.0 35.0 31.0 40.0 64-65 34.48255845974126 35.0 35.0 35.0 31.0 39.5 66-67 34.39977196990518 35.0 34.0 35.0 31.0 39.0 68-69 34.31102903820958 35.0 33.5 35.0 31.0 39.0 70-71 34.252063292548414 35.0 33.0 35.0 31.0 38.0 72-73 34.20260722025918 35.0 33.0 35.0 31.0 37.5 74-75 34.12973165679048 35.0 33.5 35.0 31.0 37.0 76-77 33.54624051466659 35.0 33.0 35.0 29.5 36.0 78-79 34.102287331403424 35.0 33.5 35.0 31.5 36.0 80-81 34.351478481245266 35.0 34.5 35.0 32.0 36.0 82-83 34.21863932771275 35.0 34.0 35.0 32.0 36.0 84-85 33.97716603524955 35.0 33.5 35.0 31.0 36.0 86-87 34.09328853528207 35.0 34.0 35.0 31.5 35.0 88-89 33.96626585557458 35.0 34.0 35.0 31.5 35.0 90-91 33.95127995215665 35.0 34.0 35.0 31.5 35.0 92-93 33.86598550698005 35.0 34.0 35.0 31.0 35.0 94-95 33.90793773119606 35.0 34.0 35.0 31.0 35.0 96-97 33.869318776492754 35.0 34.0 35.0 31.0 35.0 98-99 33.88649698820371 35.0 33.5 35.0 31.0 35.0 100-101 33.53833448552905 35.0 33.0 35.0 30.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 7.0 21 3.0 22 14.0 23 28.0 24 30.0 25 48.0 26 58.0 27 53.0 28 62.0 29 48.0 30 53.0 31 81.0 32 120.0 33 168.0 34 364.0 35 1407.0 36 645.0 37 462.0 38 286.0 39 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 6.2 70.42500000000001 15.625 7.75 2 7.3999999999999995 36.6 18.725 37.275000000000006 3 5.800000000000001 38.800000000000004 49.5 5.8999999999999995 4 6.1 37.775 50.2 5.925 5 7.196779063915451 38.24861600402617 48.4398590840463 6.114745848012078 6 7.3999999999999995 37.85 49.05 5.7 7 6.5 39.25 48.35 5.8999999999999995 8 6.425 38.05 49.075 6.45 9 6.325 38.15 49.475 6.05 10-11 6.3125 38.7 48.9375 6.05 12-13 6.525 38.65 49.075 5.75 14-15 5.7125 38.7125 49.5 6.075 16-17 6.3 38.824999999999996 48.912499999999994 5.9624999999999995 18-19 6.4 38.574999999999996 48.8125 6.2125 20-21 6.1125 39.387499999999996 48.675000000000004 5.825 22-23 5.5875 39.574999999999996 48.8 6.0375 24-25 5.925 39.4125 49.225 5.4375 26-27 5.8999999999999995 39.0875 49.575 5.4375 28-29 6.425 38.7875 49.1875 5.6000000000000005 30-31 5.919395465994962 39.5088161209068 48.99244332493703 5.579345088161209 32-33 5.816062176165803 39.81865284974093 48.251295336787564 6.113989637305699 34-35 6.269883351007423 40.204135737009544 47.30911983032874 6.216861081654295 36-37 6.439037505040999 40.82537975534346 46.76703858045436 5.968544159161178 38-39 6.435374149659864 40.625850340136054 47.006802721088434 5.931972789115647 40-41 6.177765547605944 40.85030269675289 46.766648321408915 6.205283434232252 42-43 6.260434056761269 40.81803005008347 47.23149693934335 5.690038953811909 44-45 6.227157717177397 41.256676974978916 46.513916221535 6.002249086308687 46-47 6.053716072189854 41.69390365212449 45.99971578797783 6.252664487707831 48-49 5.876451196789452 41.98079403755195 45.592661602407915 6.550093163250681 50-51 6.380510440835267 41.777842227378194 45.95417633410673 5.887470997679815 52-53 6.661764705882353 41.529411764705884 45.911764705882355 5.897058823529411 54-55 6.402620607504467 41.98927933293627 45.84574151280524 5.762358546754021 56-57 5.709969788519637 42.0392749244713 45.755287009063444 6.495468277945619 58-59 5.8706315144083385 42.3973022685469 45.63151440833844 6.100551808706315 60-61 6.277258566978193 42.258566978193144 45.140186915887845 6.32398753894081 62-63 6.191607284243863 42.565320665083135 45.25732383214569 5.985748218527316 64-65 6.048906048906049 42.760617760617755 45.25418275418275 5.936293436293436 66-67 6.1435124508519 42.873525557011796 44.54456094364351 6.438401048492793 68-69 5.874499332443257 43.307743658210946 43.858477970627504 6.959279038718291 70-71 6.654154581129499 43.422624125575844 43.71267701757379 6.210544275720867 72-73 6.5545040308447255 43.463021381002456 43.305292674377846 6.677181913774974 74-75 6.272401433691756 43.62007168458781 43.96057347670251 6.146953405017921 76-77 6.881405563689605 43.70424597364568 42.9904831625183 6.423865300146413 78-79 7.705263157894738 49.305263157894736 35.89473684210526 7.094736842105263 80-81 8.284920434453143 57.11038140944683 26.117706491538268 8.486991664561758 82-83 8.931835988508748 56.82945938887438 25.411334552102378 8.827370070514496 84-85 8.373721055465804 57.431340872374804 25.040387722132472 9.154550350026925 86-87 7.915859396623305 56.29670633822308 26.18322723498478 9.604207030168835 88-89 9.152542372881356 55.7909604519774 26.638418079096045 8.418079096045197 90-91 8.738712496358868 55.51995339353335 26.536556947276434 9.204777162831343 92-93 8.61344537815126 55.55222088835534 26.47058823529412 9.36374549819928 94-95 8.317815021725636 55.121042830540034 26.66045934202359 9.900682805710739 96-97 9.81457800511509 54.73145780051151 25.63938618925831 9.81457800511509 98-99 9.561231172233137 54.78061558611657 26.096922069417154 9.561231172233137 100-101 9.019473864024599 56.20088828151692 25.930987359070723 8.84865049538777 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1463.0 1 1313.5 2 826.0 3 392.0 4 237.5 5 112.0 6 40.0 7 34.5 8 24.0 9 12.0 10 8.5 11 9.0 12 14.0 13 15.5 14 14.0 15 16.0 16 17.0 17 14.5 18 13.0 19 17.0 20 18.5 21 14.0 22 15.5 23 17.5 24 12.5 25 14.5 26 16.0 27 13.0 28 14.5 29 16.0 30 16.5 31 18.0 32 18.5 33 20.0 34 26.0 35 29.5 36 31.0 37 40.0 38 40.0 39 40.0 40 48.0 41 44.0 42 48.0 43 46.5 44 35.5 45 37.5 46 34.5 47 28.5 48 32.0 49 27.5 50 21.0 51 20.0 52 20.0 53 17.5 54 11.5 55 11.0 56 12.0 57 10.0 58 9.0 59 10.0 60 7.5 61 6.0 62 8.0 63 9.0 64 6.5 65 6.5 66 5.0 67 4.0 68 4.0 69 1.5 70 2.5 71 2.5 72 3.0 73 5.0 74 4.5 75 2.5 76 2.0 77 2.0 78 1.5 79 1.0 80 1.0 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 3.5 99 13.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.65 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013253810470510271 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 115.0 32-33 98.0 34-35 53.0 36-37 49.0 38-39 41.0 40-41 41.0 42-43 34.0 44-45 45.0 46-47 27.0 48-49 35.0 50-51 52.0 52-53 41.0 54-55 47.0 56-57 47.0 58-59 54.0 60-61 50.0 62-63 49.0 64-65 58.0 66-67 52.0 68-69 63.0 70-71 79.0 72-73 65.0 74-75 59.0 76-77 64.0 78-79 688.0 80-81 65.0 82-83 61.0 84-85 54.0 86-87 31.0 88-89 57.0 90-91 43.0 92-93 61.0 94-95 46.0 96-97 41.0 98-99 40.0 100-101 1495.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.875 #Duplication Level Percentage of deduplicated Percentage of total 1 90.25089605734767 31.474999999999998 2 1.8637992831541221 1.3 3 0.7885304659498209 0.8250000000000001 4 0.6451612903225806 0.8999999999999999 5 0.2867383512544803 0.5 6 0.6451612903225806 1.35 7 0.7885304659498209 1.925 8 1.003584229390681 2.8000000000000003 9 0.6451612903225806 2.025 >10 2.867383512544803 15.325 >50 0.0 0.0 >100 0.07168458781362007 7.000000000000001 >500 0.14336917562724014 34.575 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 739 18.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 644 16.1 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 7.000000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.8999999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.8999999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.8750000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.8 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.5 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.44999999999999996 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.42500000000000004 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.35000000000000003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.35000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.35000000000000003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.35000000000000003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.3 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.3 No Hit AAAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.3 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.22499999999999998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.22499999999999998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 120 0.0 66.256775 1 CTTTTTT 120 0.0 66.256775 2 >>END_MODULE SRR13172450 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172450_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 12 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.073 39.0 34.0 41.0 31.0 41.0 2 37.54525 40.0 34.0 41.0 33.0 41.0 3 37.3595 40.0 34.0 41.0 31.0 41.0 4 38.686 40.0 37.0 41.0 36.0 41.0 5 38.758 40.0 37.0 41.0 36.0 41.0 6 38.64 40.0 37.0 41.0 36.0 41.0 7 38.63525 40.0 37.0 41.0 35.0 41.0 8 38.51725 40.0 37.0 41.0 35.0 41.0 9 38.9725 40.0 39.0 41.0 36.0 41.0 10-11 38.98375 39.5 39.0 41.0 36.0 41.0 12-13 38.6195 39.0 37.5 41.0 35.5 41.0 14-15 37.293625 37.0 36.0 40.0 34.5 41.0 16-17 36.269375 35.0 35.0 39.5 33.0 41.0 18-19 35.54025 35.0 35.0 36.0 33.0 41.0 20-21 35.237875 35.0 35.0 35.0 33.0 40.5 22-23 35.1 35.0 35.0 35.0 33.0 40.0 24-25 35.086125 35.0 35.0 35.0 33.0 40.0 26-27 35.1395 35.0 35.0 35.0 33.0 40.0 28-29 34.803875000000005 35.0 35.0 35.0 33.0 40.0 30-31 34.94938752556237 35.0 35.0 35.0 33.0 40.0 32-33 34.878064407641276 35.0 35.0 35.0 33.0 40.0 34-35 34.85574330259015 35.0 35.0 35.0 33.0 40.0 36-37 34.83277144155409 35.0 35.0 35.0 33.0 40.0 38-39 34.70244061349938 35.0 35.0 35.0 33.0 40.0 40-41 34.72085074227647 35.0 35.0 35.0 33.0 39.5 42-43 34.71284095943295 35.0 35.0 35.0 33.0 39.0 44-45 34.66656559034557 35.0 35.0 35.0 33.0 39.0 46-47 34.64994217363471 35.0 35.0 35.0 33.0 38.5 48-49 34.59802605646293 35.0 35.0 35.0 33.0 38.5 50-51 34.34393100832307 35.0 34.5 35.0 32.0 37.5 52-53 34.38882525425019 35.0 35.0 35.0 32.5 38.0 54-55 34.57740440595712 35.0 35.0 35.0 33.0 38.0 56-57 34.44620488160281 35.0 35.0 35.0 33.0 38.0 58-59 34.51002951828221 35.0 35.0 35.0 33.0 37.5 60-61 34.51300826581131 35.0 35.0 35.0 33.0 37.0 62-63 34.44128281206933 35.0 35.0 35.0 33.0 37.0 64-65 34.51158351154906 35.0 35.0 35.0 33.0 36.5 66-67 34.496840790440125 35.0 35.0 35.0 33.0 36.0 68-69 34.40425374982219 35.0 35.0 35.0 32.0 36.0 70-71 34.3458859773946 35.0 35.0 35.0 31.5 36.0 72-73 34.31985008547173 35.0 35.0 35.0 32.0 36.0 74-75 34.28126445201653 35.0 34.0 35.0 32.0 35.0 76-77 34.22103584328032 35.0 34.0 35.0 31.5 35.0 78-79 34.16068267759316 35.0 33.5 35.0 31.0 35.5 80-81 34.273203469214664 35.0 34.0 35.0 31.5 37.0 82-83 34.219643701834954 35.0 34.0 35.0 31.0 36.0 84-85 34.07769404275747 35.0 34.0 35.0 31.0 36.0 86-87 33.84044032880557 35.0 33.5 35.0 31.0 36.0 88-89 33.85794223826714 35.0 33.0 35.0 31.0 35.0 90-91 33.785805659079045 35.0 33.5 35.0 31.0 35.0 92-93 33.68294823047675 35.0 33.0 35.0 31.0 35.0 94-95 33.71788079925242 35.0 33.0 35.0 31.0 35.0 96-97 33.74761772933908 35.0 33.0 35.0 31.0 35.0 98-99 33.907803180428374 35.0 33.5 35.0 31.5 35.0 100-101 33.65961192065838 35.0 33.0 35.0 30.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 0.0 21 2.0 22 4.0 23 5.0 24 14.0 25 16.0 26 22.0 27 27.0 28 29.0 29 35.0 30 46.0 31 50.0 32 107.0 33 153.0 34 323.0 35 1483.0 36 972.0 37 473.0 38 203.0 39 32.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 3.4250000000000003 88.9 3.45 4.2250000000000005 2 3.55 35.9 5.25 55.300000000000004 3 4.575 44.25 47.925000000000004 3.25 4 3.55 36.175000000000004 56.10000000000001 4.175 5 12.225 36.975 47.675 3.125 6 13.225000000000001 36.05 47.699999999999996 3.025 7 11.899999999999999 36.125 48.85 3.125 8 8.5 37.15 50.2 4.15 9 6.8500000000000005 38.324999999999996 50.0 4.825 10-11 5.575 38.75 50.8625 4.8125 12-13 5.0 38.4 51.0125 5.5875 14-15 5.4 39.4375 50.075 5.0874999999999995 16-17 5.9375 37.95 50.425 5.6875 18-19 5.375 38.9625 50.0875 5.575 20-21 5.2124999999999995 39.300000000000004 50.31250000000001 5.175 22-23 4.5875 39.45 50.74999999999999 5.2124999999999995 24-25 4.9750000000000005 39.5625 50.525 4.9375 26-27 5.025 39.5875 50.4625 4.925 28-29 5.1875 40.0625 49.8 4.95 30-31 5.460060667340748 40.16683518705763 49.62082912032356 4.752275025278059 32-33 5.478008954437714 40.8743745061891 48.867526994996055 4.78008954437714 34-35 5.138339920948617 42.70137658443506 46.981054927081914 5.179228567534415 36-37 5.853658536585367 43.10801393728223 45.93728222996516 5.101045296167247 38-39 5.5681496174553695 43.45423632757155 45.55114763389062 5.42646642108246 40-41 5.8739049260376275 43.93221312652592 45.03805830820049 5.155823639235962 42-43 5.969498910675381 44.83660130718955 43.96514161220043 5.228758169934641 44-45 5.935066842955781 44.468929043631555 44.468929043631555 5.127075069781108 46-47 5.813261968480524 44.51382694023194 44.320547130538216 5.352363960749331 48-49 5.291085506367495 45.46694966646452 44.072164948453604 5.169799878714372 50-51 5.319969159599075 45.81341557440247 43.885890516576715 4.980724749421742 52-53 5.353788353162179 45.75767063243582 43.722604884157796 5.165936130244208 54-55 5.503270059020577 45.92438985484128 43.46785771255384 5.104482373584304 56-57 5.69620253164557 45.5533917559234 43.411230120090885 5.339175592340149 58-59 5.911330049261084 45.878489326765184 43.251231527093594 4.958949096880132 60-61 5.900983497249541 45.340890148358056 43.40723453908985 5.350891815302551 62-63 5.645981688708036 45.676500508647 43.28585961342828 5.391658189216684 64-65 5.738557558945908 45.70041608876561 43.3252427184466 5.235783633841886 66-67 5.600850762141085 45.53349875930521 43.08755760368664 5.778092874867069 68-69 5.443658138268917 45.65414625294865 43.11377245508982 5.788423153692615 70-71 5.703139513282556 45.550808099572734 42.968604867174435 5.777447519970277 72-73 5.829681844160793 45.627738616879405 42.674795199085544 5.8677843398742615 74-75 5.948174322732626 45.72045543776993 42.736552807224186 5.5948174322732624 76-77 5.638041733547351 45.04414125200642 43.59951845906902 5.718298555377207 78-79 7.272242940863078 55.221097496004255 31.806073521576984 5.700586041555674 80-81 7.266602502406159 72.42540904716073 12.897016361886429 7.41097208854668 82-83 9.433962264150944 67.81354051054383 13.429522752497224 9.32297447280799 84-85 9.51834862385321 66.68577981651376 14.105504587155963 9.690366972477065 86-87 9.616519174041297 65.48672566371681 15.280235988200591 9.616519174041297 88-89 10.115081768625075 66.2023016353725 14.415505754088432 9.267110841913992 90-91 10.359801488833748 64.45409429280396 15.074441687344914 10.11166253101737 92-93 10.325047801147228 63.79859783301466 15.806246016571066 10.070108349267048 94-95 10.747051114023591 64.41677588466578 16.25163826998689 8.584534731323721 96-97 10.053981106612685 64.23751686909581 15.991902834008098 9.7165991902834 98-99 11.258741258741258 63.286713286713294 14.685314685314685 10.76923076923077 100-101 9.177679882525698 65.85903083700441 15.491923641703378 9.47136563876652 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1216.0 1 1369.0 2 1114.0 3 590.0 4 380.0 5 157.0 6 25.5 7 20.0 8 12.0 9 19.0 10 20.5 11 14.0 12 16.5 13 14.0 14 10.0 15 8.0 16 12.5 17 15.0 18 13.5 19 11.5 20 7.5 21 7.0 22 8.5 23 9.0 24 10.0 25 10.0 26 9.0 27 12.5 28 13.5 29 11.5 30 8.0 31 7.0 32 12.0 33 15.0 34 19.5 35 25.0 36 34.0 37 37.0 38 39.0 39 45.0 40 46.0 41 41.0 42 38.0 43 44.5 44 46.0 45 36.5 46 35.5 47 35.5 48 25.5 49 23.0 50 23.5 51 18.5 52 17.0 53 18.0 54 16.0 55 15.5 56 19.5 57 19.0 58 11.5 59 7.0 60 7.0 61 8.0 62 8.5 63 10.5 64 11.5 65 8.5 66 7.5 67 8.0 68 6.0 69 5.0 70 4.5 71 3.5 72 2.5 73 2.0 74 1.5 75 2.5 76 3.5 77 1.5 78 1.0 79 1.0 80 1.5 81 2.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 12.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 158.0 32-33 149.0 34-35 90.0 36-37 61.0 38-39 48.0 40-41 44.0 42-43 34.0 44-45 46.0 46-47 41.0 48-49 75.0 50-51 47.0 52-53 58.0 54-55 60.0 56-57 32.0 58-59 44.0 60-61 52.0 62-63 60.0 64-65 66.0 66-67 59.0 68-69 69.0 70-71 64.0 72-73 81.0 74-75 55.0 76-77 72.0 78-79 1279.0 80-81 252.0 82-83 25.0 84-85 27.0 86-87 21.0 88-89 20.0 90-91 22.0 92-93 21.0 94-95 22.0 96-97 25.0 98-99 26.0 100-101 695.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.000000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 89.28571428571429 25.0 2 2.232142857142857 1.25 3 0.7142857142857143 0.6 4 0.4464285714285714 0.5 5 0.35714285714285715 0.5 6 0.625 1.05 7 0.26785714285714285 0.525 8 0.08928571428571429 0.2 9 0.26785714285714285 0.675 >10 5.267857142857143 22.875 >50 0.26785714285714285 5.325 >100 0.0 0.0 >500 0.17857142857142858 41.5 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 851 21.275 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 809 20.225 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 2.025 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75 1.875 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 1.425 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 1.175 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.975 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.8500000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.625 No Hit AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.625 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.5 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.5 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.5 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.5 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.5 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.475 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.44999999999999996 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.42500000000000004 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.4 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.375 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.35000000000000003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.35000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.35000000000000003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.35000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.35000000000000003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.35000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.35000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGGG 15 0.0031275116 63.05 1 CTTTTTT 210 0.0 60.04762 2 ACTTTTT 210 0.0 58.54643 1 >>END_MODULE Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750670 spots for SRR13172450.sra Written 750670 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra Read 750667 spots for SRR13172450.sra Written 750667 spots for SRR13172450.sra SRR ids: ['SRR13172450.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__4rsy_m8 SRR13172450.sra spots: 15013343 blocks: [[1, 750667], [750668, 1501334], [1501335, 2252001], [2252002, 3002668], [3002669, 3753335], [3753336, 4504002], [4504003, 5254669], [5254670, 6005336], [6005337, 6756003], [6756004, 7506670], [7506671, 8257337], [8257338, 9008004], [9008005, 9758671], [9758672, 10509338], [10509339, 11260005], [11260006, 12010672], [12010673, 12761339], [12761340, 13512006], [13512007, 14262673], [14262674, 15013343]] SRR13172450 file size 3041513 SRR13172450 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172450 SRR13172450_1.fastq SRR13172450_2.fastq Input file: SRR13172450_1.fastq Paired file: SRR13172450_2.fastq trimmed: SRR13172450-trimmed-pair1.fastq, SRR13172450-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:40:35 2024 >> started Fri Dec 6 10:40:56 2024 >> done (21.127s) 15013343 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 15013343 (100.00%) read pairs available; of these: 5506123 (36.67%) trimmed read pairs available after processing 9507220 (63.33%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 25 1 0.00% 26 2 0.00% 27 2 0.00% 28 74 0.00% 29 9683 0.06% 30 31861 0.21% 31 57750 0.38% 32 65860 0.44% 33 67712 0.45% 34 58645 0.39% 35 54912 0.37% 36 44399 0.30% 37 40527 0.27% 38 40101 0.27% 39 41716 0.28% 40 41498 0.28% 41 41682 0.28% 42 42764 0.28% 43 42791 0.29% 44 45873 0.31% 45 49591 0.33% 46 50721 0.34% 47 57802 0.39% 48 60162 0.40% 49 69073 0.46% 50 66862 0.45% 51 80076 0.53% 52 78666 0.52% 53 81001 0.54% 54 105224 0.70% 55 110049 0.73% 56 109009 0.73% 57 113419 0.76% 58 114052 0.76% 59 116638 0.78% 60 122607 0.82% 61 126527 0.84% 62 134086 0.89% 63 151522 1.01% 64 162823 1.08% 65 319074 2.13% 66 440417 2.93% 67 312104 2.08% 68 224801 1.50% 69 192123 1.28% 70 185570 1.24% 71 179835 1.20% 72 175659 1.17% 73 172858 1.15% 74 193405 1.29% 75 173947 1.16% 76 188166 1.25% 77 253591 1.69% 78 964382 6.42% 79 771686 5.14% 80 225639 1.50% 81 192914 1.28% 82 225986 1.51% 83 234676 1.56% 84 239022 1.59% 85 235105 1.57% 86 247745 1.65% 87 256243 1.71% 88 322715 2.15% 89 4169849 27.77% 90 154368 1.03% 91 29498 0.20% 92 16216 0.11% 93 11602 0.08% 94 12639 0.08% 95 14709 0.10% 96 16753 0.11% 97 35558 0.24% 98 61773 0.41% 99 78880 0.53% 100 333334 2.22% 101 462738 3.08% 15013343 reads passed initial QC criterion=sequence-density sequence-density=0.08 sequence-density-rank=1 fanout-score=3.33 fanout-score-rank=17 prefix-density=0.04 prefix-fanout=3.3 sequence=TGTGGTATCAAC criterion=fanout-score sequence-density=0.02 sequence-density-rank=18 fanout-score=19.65 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=1.2 sequence=TATCAACGCAGG criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=4.49 fanout-score-rank=20 prefix-density=0.64 prefix-fanout=1.2 sequence=TATCAACGCAGAGTACATGGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=45 fanout-score=83.36 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=2.7 sequence=GTACATGGGCAGAGTACAT SRR13172450 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:42:49 Started mapping on | Dec 06 10:42:49 Finished on | Dec 06 10:56:18 Mapping speed, Million of reads per hour | 66.81 Number of input reads | 15013343 Average input read length | 155 UNIQUE READS: Uniquely mapped reads number | 2182646 Uniquely mapped reads % | 14.54% Average mapped length | 147.76 Number of splices: Total | 602161 Number of splices: Annotated (sjdb) | 263512 Number of splices: GT/AG | 517959 Number of splices: GC/AG | 4705 Number of splices: AT/AC | 109 Number of splices: Non-canonical | 79388 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.01% Deletion average length | 1.40 Insertion rate per base | 0.00% Insertion average length | 1.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2312930 % of reads mapped to multiple loci | 15.41% Number of reads mapped to too many loci | 581060 % of reads mapped to too many loci | 3.87% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 61.86% % of reads unmapped: other | 4.33% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 11119554 11119554 11119554 N_multimapping 2312930 2312930 2312930 N_noFeature 173634 1416347 888863 N_ambiguous 63283 6017 7151 UnstrandedReadsAssigned:1945729 PositiveStrandReadsAssigned:760282 NegativeStrandReadsAssigned:1286632 Dataset is classified unstranded MeadianReadLen=78 20thPercentileLength=62 echo kmer=57 SRR13172450 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172450-trimmed-pair1.fastq SRR13172450-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,013,343 reads, 13,327,613 reads pseudoaligned [quant] estimated average fragment length: 137.218 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,065 rounds 52973 SRR13172450.ke.tsv 35125 SRR13172450.se.tsv 88098 total ==> SRR13172450.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 799.836 0 0 PNS24247 1044 907.782 0 0 PNS24249 1928 1791.78 0 0 PNS24246 1044 907.782 0 0 PNS24248 1044 907.782 0 0 PNS24244 1471 1334.78 36 4.56989 PNS24243 293 158.556 0 0 KQK14069 1603 1466.78 115 13.2845 KQK14071 474 338.299 0 0 ==> SRR13172450.se.tsv <== BRADI_1g14170v3 103 BRADI_1g53295v3 0 BRADI_1g59795v3 2 BRADI_1g07683v3 0 BRADI_1g00485v3 6 BRADI_1g20270v3 118 BRADI_1g74790v3 2 BRADI_1g09890v3 0 BRADI_1g77505v3 0 BRADI_1g48960v3 0 SRR13172450 completed mapping pipeline successfully