Starting /dee2/code/volunteer_pipeline.sh SRR13172451 current disk space = 1551422537728 free memory = 1605437456 SRR13172451 SRAfilesize 2badc5b581a90b6854409caf7d060d4e SRR13172451.sra SRR13172451.sra file validated SRR13172451 is paired end SRR13172451 is conventional basespace SRR13172451 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172451_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 31 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.50375 34.0 33.0 34.0 31.0 40.0 2 34.9805 34.0 34.0 35.0 31.0 41.0 3 34.96975 34.0 34.0 35.0 31.0 41.0 4 37.349 37.0 37.0 37.0 35.0 41.0 5 37.345 37.0 37.0 37.0 35.0 41.0 6 37.43325 37.0 37.0 37.0 35.0 41.0 7 37.395 37.0 37.0 37.0 35.0 41.0 8 37.5245 37.0 37.0 37.0 35.0 41.0 9 38.908 39.0 39.0 39.0 37.0 41.0 10-11 38.855625 39.0 39.0 39.0 37.0 41.0 12-13 38.838625 39.0 39.0 39.0 37.0 41.0 14-15 39.068875 40.0 37.0 41.0 36.5 41.0 16-17 38.475 40.0 35.0 41.0 35.0 41.0 18-19 38.21525 40.0 35.0 41.0 35.0 41.0 20-21 38.113125 40.0 35.0 41.0 35.0 41.0 22-23 37.922 39.5 35.0 41.0 35.0 41.0 24-25 37.866125 39.5 35.0 41.0 35.0 41.0 26-27 37.865125 40.0 35.0 41.0 35.0 41.0 28-29 37.79625 40.0 35.0 41.0 35.0 41.0 30-31 37.6871879164531 39.5 35.0 41.0 34.5 41.0 32-33 37.71522228246879 40.0 35.0 41.0 33.5 41.0 34-35 37.77283258844608 40.0 35.0 41.0 34.0 41.0 36-37 37.87626450939656 40.0 35.0 41.0 34.5 41.0 38-39 37.87779230398773 40.0 35.0 41.0 35.0 41.0 40-41 37.831813659831 40.0 35.0 41.0 35.0 41.0 42-43 37.717362372563386 39.5 35.0 41.0 34.0 41.0 44-45 37.660477845586236 39.0 35.0 41.0 34.0 41.0 46-47 37.670921706398445 39.0 35.0 41.0 34.0 41.0 48-49 37.591133478510045 39.0 35.0 41.0 34.0 41.0 50-51 37.52613295420477 39.0 35.0 41.0 33.5 41.0 52-53 37.36030462472823 39.0 35.0 41.0 33.5 41.0 54-55 37.38972835713278 39.0 35.0 41.0 33.5 41.0 56-57 37.28493471768873 38.0 35.0 41.0 33.0 41.0 58-59 37.215843967873695 38.0 35.0 40.5 33.5 41.0 60-61 37.009484212687084 37.5 35.0 40.0 33.0 41.0 62-63 36.769464539111546 37.0 35.0 40.0 33.0 41.0 64-65 36.56401749181205 36.5 35.0 40.0 33.0 41.0 66-67 36.43416001557759 36.0 35.0 39.5 33.0 41.0 68-69 36.143750139724126 35.0 35.0 39.0 33.0 41.0 70-71 35.82791861532985 35.0 35.0 39.0 33.0 40.5 72-73 35.538725989696836 35.0 35.0 37.5 32.5 40.0 74-75 35.048178534330376 35.0 34.5 37.0 31.5 39.5 76-77 34.007111911786 34.5 32.5 35.5 30.0 39.0 78-79 34.74367268797462 35.0 34.0 36.0 31.5 39.0 80-81 34.83277291037389 35.0 34.0 36.0 32.0 38.5 82-83 34.60723492866944 35.0 34.0 36.0 32.0 37.0 84-85 34.42356326087913 35.0 34.0 35.0 32.0 37.0 86-87 34.407205314847005 35.0 34.0 35.0 32.0 36.5 88-89 34.24809395813719 35.0 34.0 35.0 32.0 36.0 90-91 33.93218019161435 35.0 34.0 35.0 31.0 36.0 92-93 33.784164672455574 35.0 34.0 35.0 31.0 35.5 94-95 33.59988567169459 35.0 33.5 35.0 30.5 35.0 96-97 33.72225053414235 35.0 34.0 35.0 31.0 35.0 98-99 33.42539724932228 35.0 33.0 35.0 29.5 35.0 100-101 33.41203052238992 34.5 33.0 35.0 30.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 4.0 24 5.0 25 9.0 26 14.0 27 13.0 28 14.0 29 30.0 30 34.0 31 41.0 32 62.0 33 99.0 34 203.0 35 597.0 36 627.0 37 1145.0 38 947.0 39 153.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.15 51.425 21.675 14.75 2 14.825 26.55 22.225 36.4 3 13.675 28.849999999999998 44.25 13.225000000000001 4 14.575 25.775 45.324999999999996 14.325 5 14.623115577889449 25.804020100502512 44.84924623115578 14.723618090452263 6 14.274999999999999 26.650000000000002 43.75 15.325 7 16.325 25.624999999999996 44.5 13.55 8 15.375 27.025 44.375 13.225000000000001 9 15.4 25.575 44.625 14.399999999999999 10-11 14.3375 27.187499999999996 44.1 14.374999999999998 12-13 14.45 25.662499999999998 44.9125 14.975 14-15 15.312500000000002 26.487500000000004 44.175 14.025000000000002 16-17 14.662500000000001 27.05 44.25 14.0375 18-19 14.725 25.8 44.775 14.7 20-21 15.45 26.375 44.3125 13.8625 22-23 14.45 26.8375 44.6375 14.075 24-25 14.524999999999999 25.95 44.1375 15.387500000000001 26-27 14.575 26.6125 44.3625 14.45 28-29 14.625 27.0 43.9375 14.4375 30-31 14.021766641356617 26.955201214882308 44.1280688433308 14.89496330043027 32-33 15.68075117370892 28.665325285043597 40.36217303822938 15.29175050301811 34-35 16.379310344827587 30.367074527252502 37.08286985539488 16.17074527252503 36-37 16.62674369451881 31.041285050021134 36.184303226715514 16.14766802874454 38-39 16.522601672098624 30.622077369987245 36.3327192858155 16.522601672098624 40-41 16.73079663673935 31.409434231152915 35.314236853356135 16.545532278751605 42-43 16.60458452722063 31.3323782234957 35.372492836676216 16.69054441260745 44-45 16.609046384327282 32.238547968885044 34.80265053298761 16.349755113800057 46-47 15.723998264140027 33.31404600028931 34.68826847967597 16.273687255894693 48-49 16.043636363636363 32.654545454545456 34.981818181818184 16.32 50-51 16.963243873978996 32.424154025670944 34.495332555425904 16.117269544924152 52-53 15.946697905989163 33.62132083760434 33.679894567286574 16.75208668911993 54-55 16.00880410858401 34.67351430667645 33.749082905355834 15.568598679383713 56-57 16.234531526222746 34.502062463170304 33.014142604596344 16.249263406010606 58-59 15.901687888658572 34.79419603198105 32.89902280130293 16.405093278057446 60-61 15.815338793745346 35.60685033507074 32.271034996276995 16.306775874906926 62-63 16.382405745062837 36.0263315380012 32.18132854578097 15.409934171154996 64-65 16.28012048192771 35.79819277108434 32.34939759036145 15.572289156626507 66-67 15.652965266191416 36.887608069164266 31.017746094342485 16.441680570301838 68-69 15.580650083931024 36.929650541736606 31.924309476575612 15.565389897756754 70-71 16.676885346413243 36.02084610668302 30.870631514408338 16.431637032495402 72-73 16.707920792079207 37.11324257425743 30.78589108910891 15.392945544554456 74-75 15.155298891837054 38.145778055252066 30.279381926018416 16.41954112689246 76-77 15.928369462770972 37.84165881244109 29.610430411561424 16.619541313226517 78-79 16.622162883845128 40.80440587449933 25.58411214953271 16.98931909212283 80-81 16.675584091314427 42.3577670768682 23.631175316568577 17.335473515248793 82-83 17.2830597149558 43.40609778098503 22.40663900414938 16.904203499909794 84-85 16.819012797074954 42.79707495429616 22.595978062157222 17.787934186471663 86-87 17.937800814513142 42.61384672343576 21.67715660866346 17.771195853387635 88-89 17.370892018779344 42.460093896713616 22.910798122065728 17.258215962441316 90-91 16.87022900763359 42.786259541984734 23.129770992366414 17.213740458015266 92-93 17.48197232508283 42.42837653478854 22.198401870980316 17.891249269148314 94-95 18.16014305583151 41.54579773494934 21.676932247168686 18.617126962050467 96-97 17.99307958477509 41.64461632403827 22.06391207001832 18.29839202116833 98-99 17.484854815124294 42.78253603509505 22.393983705870067 17.33862544391059 100-101 15.357689783417195 43.01028221395756 22.489608400787574 19.142419601837673 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 316.0 1 454.0 2 597.5 3 561.5 4 455.5 5 200.5 6 7.5 7 7.0 8 5.5 9 4.5 10 7.0 11 6.0 12 5.0 13 6.5 14 7.5 15 11.0 16 12.0 17 14.0 18 18.0 19 19.5 20 24.0 21 24.0 22 28.0 23 34.0 24 32.0 25 28.0 26 25.5 27 31.5 28 30.0 29 28.0 30 33.5 31 36.5 32 46.5 33 52.5 34 63.5 35 73.5 36 77.0 37 86.5 38 112.0 39 127.5 40 124.5 41 127.0 42 122.0 43 125.0 44 138.0 45 113.5 46 89.0 47 90.5 48 74.5 49 62.5 50 61.0 51 55.0 52 50.5 53 50.5 54 50.0 55 39.0 56 27.5 57 27.5 58 27.5 59 20.5 60 15.0 61 17.0 62 17.0 63 17.0 64 14.0 65 10.0 66 12.5 67 11.0 68 9.0 69 8.0 70 5.0 71 5.5 72 8.5 73 8.0 74 3.5 75 4.5 76 5.5 77 3.0 78 3.0 79 2.5 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 216.0 32-33 168.0 34-35 64.0 36-37 19.0 38-39 20.0 40-41 18.0 42-43 20.0 44-45 13.0 46-47 23.0 48-49 6.0 50-51 16.0 52-53 8.0 54-55 9.0 56-57 19.0 58-59 19.0 60-61 15.0 62-63 17.0 64-65 29.0 66-67 21.0 68-69 14.0 70-71 24.0 72-73 30.0 74-75 23.0 76-77 31.0 78-79 346.0 80-81 32.0 82-83 33.0 84-85 38.0 86-87 35.0 88-89 40.0 90-91 53.0 92-93 53.0 94-95 51.0 96-97 75.0 98-99 61.0 100-101 2341.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.35 #Duplication Level Percentage of deduplicated Percentage of total 1 97.79673063255153 68.8 2 0.42643923240938164 0.6 3 0.35536602700781805 0.75 4 0.21321961620469082 0.6 5 0.24875621890547264 0.8750000000000001 6 0.21321961620469082 0.8999999999999999 7 0.14214641080312723 0.7000000000000001 8 0.10660980810234541 0.6 9 0.10660980810234541 0.675 >10 0.17768301350390903 2.5250000000000004 >50 0.07107320540156362 3.8 >100 0.14214641080312723 19.175 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 302 7.55 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 235 5.875 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 2.9499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 2.8000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 2.4250000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 1.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.975 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.6 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 100 0.0 80.31645 1 CTTTTTT 105 0.0 76.49186 2 >>END_MODULE SRR13172451 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172451_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 31 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.22175 34.0 34.0 41.0 31.0 41.0 2 36.54475 34.0 34.0 41.0 31.0 41.0 3 36.3975 34.0 34.0 41.0 31.0 41.0 4 38.31625 37.0 37.0 41.0 36.0 41.0 5 38.26425 37.0 37.0 41.0 35.0 41.0 6 38.3195 37.0 37.0 41.0 36.0 41.0 7 38.2795 37.0 37.0 41.0 36.0 41.0 8 37.96825 37.0 37.0 41.0 35.0 41.0 9 38.81875 39.0 39.0 41.0 37.0 41.0 10-11 38.900625 39.0 39.0 41.0 37.0 41.0 12-13 38.92 39.0 39.0 41.0 37.0 41.0 14-15 38.511375 39.5 37.0 41.0 36.0 41.0 16-17 37.879999999999995 39.5 35.0 41.0 35.0 41.0 18-19 37.596625 39.0 35.0 41.0 35.0 41.0 20-21 37.593375 39.0 35.0 41.0 35.0 41.0 22-23 37.4195 38.0 35.0 41.0 35.0 41.0 24-25 37.159875 38.0 35.0 40.5 34.5 41.0 26-27 37.32225 38.0 35.0 41.0 35.0 41.0 28-29 37.089749999999995 38.0 35.0 41.0 33.5 41.0 30-31 37.13715396498455 38.0 35.0 41.0 33.0 41.0 32-33 37.1553229758692 38.5 35.0 41.0 33.0 41.0 34-35 37.25367015565312 39.0 35.0 41.0 33.0 41.0 36-37 37.253807519892646 38.5 35.0 41.0 33.5 41.0 38-39 37.17317019926733 38.0 35.0 41.0 34.0 41.0 40-41 37.12669115752473 38.0 35.0 41.0 34.0 41.0 42-43 36.99176296766977 37.5 35.0 40.0 33.5 41.0 44-45 36.899087277148766 37.0 35.0 40.0 33.0 41.0 46-47 36.61883131028588 36.5 35.0 40.0 33.0 41.0 48-49 36.700664849546826 36.5 35.0 40.0 33.0 41.0 50-51 36.214701705375205 35.5 35.0 39.5 33.0 40.5 52-53 36.42384208283485 36.0 35.0 39.5 33.0 40.5 54-55 36.51050754518118 35.5 35.0 40.0 33.0 41.0 56-57 36.43892227599386 35.0 35.0 40.0 33.0 41.0 58-59 36.3035402928129 35.0 35.0 40.0 33.0 41.0 60-61 36.21319805666279 35.0 35.0 40.0 33.0 41.0 62-63 36.10127780218025 35.0 35.0 39.0 33.0 41.0 64-65 35.88226050594204 35.0 35.0 39.0 33.0 41.0 66-67 35.84406221652787 35.0 35.0 39.0 33.0 41.0 68-69 35.51889522144415 35.0 35.0 37.5 33.0 41.0 70-71 35.30635919707532 35.0 35.0 37.0 32.5 40.0 72-73 35.145262049126856 35.0 35.0 37.0 32.0 39.5 74-75 34.99704158090644 35.0 35.0 36.0 32.5 39.0 76-77 34.95078464560348 35.0 34.5 36.0 33.0 39.0 78-79 34.67198812721247 35.0 34.0 36.0 31.5 38.5 80-81 34.73731106500546 35.0 34.0 36.0 32.0 38.0 82-83 34.65062251646621 35.0 34.0 36.0 32.5 37.0 84-85 34.35445491925019 35.0 34.0 35.0 31.5 37.0 86-87 34.063414860831834 35.0 34.0 35.0 31.0 36.0 88-89 33.89704617088338 35.0 34.0 35.0 31.0 36.0 90-91 33.834623626733546 35.0 34.0 35.0 31.0 36.0 92-93 33.83636305915708 35.0 34.0 35.0 31.0 35.0 94-95 33.88995170073635 35.0 34.0 35.0 31.0 35.0 96-97 33.8181842083123 35.0 34.0 35.0 31.0 35.0 98-99 33.82111469434304 35.0 34.0 35.0 31.0 35.0 100-101 33.440030623276584 34.5 33.0 35.0 30.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 3.0 23 5.0 24 11.0 25 9.0 26 16.0 27 14.0 28 11.0 29 28.0 30 24.0 31 47.0 32 52.0 33 102.0 34 186.0 35 686.0 36 734.0 37 1192.0 38 780.0 39 96.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.225 67.675 13.625000000000002 10.475 2 10.6 19.025 14.399999999999999 55.974999999999994 3 9.775 37.9 43.525000000000006 8.799999999999999 4 9.975000000000001 18.95 61.275 9.8 5 28.675 18.25 43.5 9.575 6 28.625 19.025 42.5 9.85 7 28.325 18.175 43.8 9.700000000000001 8 18.825 22.225 47.449999999999996 11.5 9 15.2 25.074999999999996 47.099999999999994 12.625 10-11 15.225 23.2875 47.7375 13.750000000000002 12-13 14.224999999999998 23.825 48.475 13.475000000000001 14-15 14.9375 23.8375 48.425000000000004 12.8 16-17 14.224999999999998 23.4125 48.5 13.8625 18-19 14.6875 23.425 48.35 13.5375 20-21 13.950000000000001 24.175 48.55 13.325000000000001 22-23 15.35 22.9625 48.512499999999996 13.175 24-25 14.887500000000001 23.95 48.1375 13.025 26-27 14.2875 23.7125 49.125 12.875 28-29 14.787500000000001 23.1375 48.575 13.5 30-31 14.751395230847287 24.08675799086758 47.666159309994924 13.495687468290207 32-33 15.918644067796611 25.993220338983054 43.905084745762714 14.183050847457626 34-35 16.0711743772242 27.62989323843416 41.65124555160142 14.647686832740215 36-37 17.424242424242426 27.636946386946388 39.86013986013986 15.078671328671328 38-39 16.661769027328827 28.665883044372613 39.494563620335 15.177784307963563 40-41 16.4079822616408 29.785661492978566 37.91574279379157 15.890613451589061 42-43 16.242748772869255 30.060984679458574 37.92949576082106 15.766770786851108 44-45 16.714093155608804 29.60910588587689 38.16085068144376 15.515950277070539 46-47 16.646543917899184 30.35013582855418 36.99064292182312 16.012677331723513 48-49 16.52477956825783 31.027667984189723 36.652477956825784 15.795074490726666 50-51 17.65515132986854 30.892693365943135 36.19688168755732 15.255273616631 52-53 17.125288239815525 30.960799385088393 35.98770176787087 15.926210607225212 54-55 16.687306501547987 31.362229102167184 36.8266253869969 15.123839009287925 56-57 17.654378596982422 30.424638357442834 36.58422771815212 15.336755327422615 58-59 17.469502658742574 31.717234907725995 35.736628088833285 15.076634344698153 60-61 17.69146092152854 32.20632174870263 35.46155055826388 14.640666771504954 62-63 16.303317535545023 32.98578199052133 36.145339652448655 14.56556082148499 64-65 16.966703839413732 33.05719292655727 34.87334714035367 15.102756093675323 66-67 16.119737473987513 32.83175924443733 35.26492716503922 15.783576116535938 68-69 16.52173913043478 34.31561996779388 33.687600644122384 15.475040257648953 70-71 16.437246963562753 33.68421052631579 34.05668016194332 15.821862348178136 72-73 17.511445389143233 33.534990189666445 33.64944408109876 15.304120340091561 74-75 16.832339297548046 33.880053015241884 33.631544068919816 15.656063618290258 76-77 16.89291101055807 34.18803418803419 33.61823361823362 15.300821183174124 78-79 16.560378522937665 39.23061098539395 28.327504628677225 15.881505862991155 80-81 16.69739267843034 43.24466684224388 22.91282591519621 17.145114564129578 82-83 16.688847484695234 42.42746872504658 23.822198562682992 17.061485227575194 84-85 18.13290287866559 42.69572235673931 22.518159806295397 16.653214958299703 86-87 17.734926670287887 42.857142857142854 22.976643128734384 16.43128734383487 88-89 16.878102592388306 43.1880860452289 22.9453943739658 16.988416988416986 90-91 16.671320860094944 43.954202736665735 22.842781345992737 16.53169505724658 92-93 17.245304496300513 44.02390438247012 22.225384177575414 16.505406943653956 94-95 16.67633410672854 44.7215777262181 22.447795823665892 16.15429234338747 96-97 17.34754292480758 43.6056838365897 22.202486678507995 16.84428656009473 98-99 15.379975874547647 45.92882991556092 21.53196622436671 17.159227985524726 100-101 17.147385103011093 46.37083993660856 20.760697305863708 15.721077654516641 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 241.0 1 497.0 2 755.5 3 701.5 4 553.0 5 233.5 6 5.5 7 6.5 8 9.0 9 9.0 10 10.0 11 10.0 12 10.0 13 11.0 14 10.5 15 10.0 16 7.5 17 7.0 18 9.5 19 13.0 20 11.5 21 12.0 22 16.5 23 16.0 24 16.0 25 20.5 26 21.0 27 25.5 28 26.0 29 27.0 30 38.5 31 39.0 32 37.5 33 44.0 34 49.5 35 63.0 36 69.0 37 76.5 38 104.0 39 117.0 40 122.5 41 130.5 42 132.5 43 129.5 44 127.5 45 133.5 46 134.0 47 117.5 48 95.5 49 79.5 50 73.0 51 72.5 52 67.5 53 57.5 54 43.0 55 37.0 56 37.0 57 32.0 58 26.0 59 24.0 60 25.5 61 21.0 62 21.5 63 24.0 64 18.5 65 14.5 66 13.5 67 10.0 68 12.0 69 11.0 70 8.0 71 11.5 72 8.0 73 5.0 74 6.5 75 4.0 76 2.0 77 3.5 78 3.5 79 2.5 80 2.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 257.0 32-33 197.0 34-35 109.0 36-37 28.0 38-39 21.0 40-41 20.0 42-43 24.0 44-45 25.0 46-47 24.0 48-49 18.0 50-51 20.0 52-53 23.0 54-55 16.0 56-57 17.0 58-59 18.0 60-61 13.0 62-63 25.0 64-65 20.0 66-67 16.0 68-69 19.0 70-71 24.0 72-73 39.0 74-75 36.0 76-77 70.0 78-79 1018.0 80-81 20.0 82-83 20.0 84-85 16.0 86-87 27.0 88-89 24.0 90-91 30.0 92-93 33.0 94-95 35.0 96-97 33.0 98-99 41.0 100-101 1624.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.35 #Duplication Level Percentage of deduplicated Percentage of total 1 97.73926149208741 64.85 2 0.4144687264506406 0.5499999999999999 3 0.18839487565938207 0.375 4 0.22607385079125847 0.6 5 0.22607385079125847 0.75 6 0.15071590052750566 0.6 7 0.22607385079125847 1.05 8 0.26375282592313487 1.4000000000000001 9 0.07535795026375283 0.44999999999999996 >10 0.22607385079125847 2.65 >50 0.07535795026375283 3.7249999999999996 >100 0.18839487565938207 23.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 356 8.9 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 5.025 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 3.5000000000000004 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 2.85 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 2.725 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 2.075 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66 1.6500000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42 1.05 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.35000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTG 15 0.0017114627 73.4 3 ATGGGGA 25 1.7779403E-6 73.4 3 ACTTTTT 100 0.0 73.4 1 TGGGGAG 15 0.0017114627 73.4 4 CATGGGG 35 1.7953425E-9 73.4 2 CTTTTTT 105 0.0 69.90476 2 ACATGGG 85 0.0 69.08235 1 CATGGGT 25 1.671749E-4 58.72 2 CATGGGC 20 0.005353406 55.05 2 >>END_MODULE Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519742 spots for SRR13172451.sra Written 1519742 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra Read 1519736 spots for SRR13172451.sra Written 1519736 spots for SRR13172451.sra SRR ids: ['SRR13172451.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8e3j9wsq SRR13172451.sra spots: 30394726 blocks: [[1, 1519736], [1519737, 3039472], [3039473, 4559208], [4559209, 6078944], [6078945, 7598680], [7598681, 9118416], [9118417, 10638152], [10638153, 12157888], [12157889, 13677624], [13677625, 15197360], [15197361, 16717096], [16717097, 18236832], [18236833, 19756568], [19756569, 21276304], [21276305, 22796040], [22796041, 24315776], [24315777, 25835512], [25835513, 27355248], [27355249, 28874984], [28874985, 30394726]] SRR13172451 file size 6409193 SRR13172451 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172451 SRR13172451_1.fastq SRR13172451_2.fastq Input file: SRR13172451_1.fastq Paired file: SRR13172451_2.fastq trimmed: SRR13172451-trimmed-pair1.fastq, SRR13172451-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:45:49 2024 >> started Fri Dec 6 10:46:28 2024 >> done (39.571s) 30394726 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 49 ( 0.00%) empty read pairs filtered out after trimming by size control 30394676 (100.00%) read pairs available; of these: 2113720 ( 6.95%) trimmed read pairs available after processing 28280956 (93.05%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 25 2 0.00% 26 1 0.00% 27 2 0.00% 28 10 0.00% 29 626 0.00% 30 2605 0.01% 31 5284 0.02% 32 6645 0.02% 33 7558 0.02% 34 8115 0.03% 35 8588 0.03% 36 9430 0.03% 37 10108 0.03% 38 11336 0.04% 39 12917 0.04% 40 13891 0.05% 41 15181 0.05% 42 16990 0.06% 43 19142 0.06% 44 21967 0.07% 45 24947 0.08% 46 28170 0.09% 47 32617 0.11% 48 37742 0.12% 49 42326 0.14% 50 48275 0.16% 51 55477 0.18% 52 62884 0.21% 53 71317 0.23% 54 98975 0.33% 55 106524 0.35% 56 110896 0.36% 57 118787 0.39% 58 130548 0.43% 59 142939 0.47% 60 158609 0.52% 61 174326 0.57% 62 200256 0.66% 63 254625 0.84% 64 323886 1.07% 65 1284315 4.23% 66 2098608 6.90% 67 1155233 3.80% 68 518656 1.71% 69 343897 1.13% 70 300979 0.99% 71 288024 0.95% 72 279061 0.92% 73 273346 0.90% 74 284114 0.93% 75 275869 0.91% 76 274649 0.90% 77 310742 1.02% 78 502837 1.65% 79 414251 1.36% 80 379142 1.25% 81 360183 1.19% 82 422565 1.39% 83 448016 1.47% 84 473728 1.56% 85 481351 1.58% 86 556733 1.83% 87 580450 1.91% 88 876776 2.88% 89 8557348 28.15% 90 237344 0.78% 91 58755 0.19% 92 46041 0.15% 93 45113 0.15% 94 51486 0.17% 95 61400 0.20% 96 75507 0.25% 97 115560 0.38% 98 282883 0.93% 99 363318 1.20% 100 1145947 3.77% 101 3775925 12.42% 30394676 reads passed initial QC criterion=sequence-density sequence-density=0.09 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=40 prefix-density=0.00 prefix-fanout=1.0 sequence=ACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=24 fanout-score=218.91 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=15.4 sequence=GAAGAAGAAGGCTGAGGTCAAGCAGCTCTAGATCATTCTCGTAGTTTTACCTGGAACTTGTTTCAGTTTCATGTCAATCTAGCTCTTGGATACAATGTCTGGTTTCAGTTGGCACTGTGCAAGTTAAGACTTGATGTCACATGGCTTGGTTTCTCGTGAACATGTTTGAGTTTTAATCTGTGTTGAATGACTGCAATATGCCCCCGCCACTTTCTC criterion=sequence-density sequence-density=0.14 sequence-density-rank=1 fanout-score=1.49 fanout-score-rank=24 prefix-density=0.03 prefix-fanout=1.5 sequence=ACATGGGGAAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=46 fanout-score=117.19 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=10.8 sequence=CCGCCGCCGCCCTACCACCACTAAGATCCATCACTCTCTTTAGTTGGGAGCACAGAATGTGATCGAAAAGACGTGAGCCGACTGTCTGAAGATGACGAGCCTTTGATTCTCCTCCCGTGCGTCGCCGCCAACCTAGGGCCAAGATCGAATAATGCAGACTGCTCCCTTCAGTTTATCTTACAGCTTATAATTTGTATTCGCCCAGTCTTAGCGTGGAAGTGCTGGCATTGTTAGCGTCGTCCCTGTGTTACTGTAAAACCATTTAGTAACTTTGGAAATGGAAGTAAAATCTGTTCACCCT SRR13172451 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:47:44 Started mapping on | Dec 06 10:47:44 Finished on | Dec 06 11:02:49 Mapping speed, Million of reads per hour | 120.91 Number of input reads | 30394676 Average input read length | 165 UNIQUE READS: Uniquely mapped reads number | 13545508 Uniquely mapped reads % | 44.57% Average mapped length | 162.53 Number of splices: Total | 3328769 Number of splices: Annotated (sjdb) | 2872884 Number of splices: GT/AG | 3205491 Number of splices: GC/AG | 44498 Number of splices: AT/AC | 2828 Number of splices: Non-canonical | 75952 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.04% Deletion average length | 1.41 Insertion rate per base | 0.00% Insertion average length | 1.13 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2152515 % of reads mapped to multiple loci | 7.08% Number of reads mapped to too many loci | 164655 % of reads mapped to too many loci | 0.54% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 47.07% % of reads unmapped: other | 0.74% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 18029257 18029257 18029257 N_multimapping 2152515 2152515 2152515 N_noFeature 681499 8420057 5451459 N_ambiguous 418898 29807 38509 UnstrandedReadsAssigned:12445111 PositiveStrandReadsAssigned:5095644 NegativeStrandReadsAssigned:8055540 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=75 echo kmer=71 SRR13172451 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172451-trimmed-pair1.fastq SRR13172451-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,394,676 reads, 23,246,328 reads pseudoaligned [quant] estimated average fragment length: 139.716 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,216 rounds 52973 SRR13172451.ke.tsv 35125 SRR13172451.se.tsv 88098 total ==> SRR13172451.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 797.333 0 0 PNS24247 1044 905.284 8.43927 0.682949 PNS24249 1928 1789.28 0 0 PNS24246 1044 905.284 8.43927 0.682949 PNS24248 1044 905.284 8.43927 0.682949 PNS24244 1471 1332.28 446.682 24.5623 PNS24243 293 155.763 1 0.470332 KQK14069 1603 1464.28 12 0.600377 KQK14071 474 335.959 0 0 ==> SRR13172451.se.tsv <== BRADI_1g14170v3 9 BRADI_1g53295v3 145 BRADI_1g59795v3 295 BRADI_1g07683v3 0 BRADI_1g00485v3 57 BRADI_1g20270v3 570 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 320 BRADI_1g48960v3 0 SRR13172451 completed mapping pipeline successfully