Starting /dee2/code/volunteer_pipeline.sh SRR13172452 current disk space = 1551418650624 free memory = 1600857168 SRR13172452 SRAfilesize 116e936b7c7fb68868a7809a67fd1861 SRR13172452.sra SRR13172452.sra file validated SRR13172452 is paired end SRR13172452 is conventional basespace SRR13172452 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172452_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.373 34.0 33.0 34.0 31.0 40.0 2 34.7445 34.0 34.0 34.0 31.0 41.0 3 34.77275 34.0 34.0 34.0 31.0 41.0 4 37.4215 37.0 37.0 37.0 35.0 41.0 5 37.234 37.0 37.0 37.0 35.0 41.0 6 37.31075 37.0 37.0 37.0 35.0 41.0 7 37.39325 37.0 37.0 37.0 35.0 41.0 8 37.392 37.0 37.0 37.0 35.0 41.0 9 38.7445 39.0 39.0 39.0 37.0 41.0 10-11 38.80225 39.0 39.0 39.0 37.0 41.0 12-13 38.817125000000004 39.0 39.0 39.0 37.0 41.0 14-15 39.125125 40.0 37.5 41.0 36.5 41.0 16-17 38.56075 40.0 35.0 41.0 35.0 41.0 18-19 38.475625 40.0 35.0 41.0 35.0 41.0 20-21 38.415 40.0 35.0 41.0 35.0 41.0 22-23 38.28125 40.0 35.0 41.0 35.0 41.0 24-25 38.262875 40.0 35.0 41.0 35.0 41.0 26-27 38.214 40.0 35.0 41.0 35.0 41.0 28-29 38.124375 40.0 35.0 41.0 35.0 41.0 30-31 38.00488717156105 40.0 35.0 41.0 34.5 41.0 32-33 38.024627571687454 40.0 35.0 41.0 34.0 41.0 34-35 38.171005789337244 40.0 36.0 41.0 34.5 41.0 36-37 38.2294646935741 40.0 36.0 41.0 35.0 41.0 38-39 38.1817802645471 40.0 35.5 41.0 35.0 41.0 40-41 38.13664137689505 40.0 35.0 41.0 35.0 41.0 42-43 38.13503250794051 40.0 35.0 41.0 35.0 41.0 44-45 38.06609281331279 40.0 35.0 41.0 35.0 41.0 46-47 37.756877286619556 39.0 35.0 41.0 33.5 41.0 48-49 37.83231127521077 39.5 35.0 41.0 34.0 41.0 50-51 37.81481834041422 39.0 35.0 41.0 34.0 41.0 52-53 37.715699663078766 39.0 35.0 41.0 33.5 41.0 54-55 37.42116009192186 39.0 35.0 41.0 33.5 41.0 56-57 37.39871065941 39.0 35.0 41.0 33.0 41.0 58-59 37.36947620765672 38.5 35.0 40.5 34.0 41.0 60-61 36.93061819707648 38.0 35.0 40.0 33.0 41.0 62-63 36.76764237479 37.0 35.0 40.0 33.0 41.0 64-65 36.525515171795995 37.0 35.0 40.0 33.0 41.0 66-67 36.21903876704012 36.0 35.0 39.5 32.5 41.0 68-69 35.97296468262848 36.0 35.0 39.0 32.0 41.0 70-71 35.6032694219022 35.0 35.0 39.0 31.5 40.5 72-73 35.35675193391668 35.0 34.5 37.5 31.0 40.0 74-75 34.97041077080955 35.0 34.0 37.0 31.0 39.0 76-77 34.10016671860393 35.0 33.0 36.0 30.0 39.0 78-79 34.602681688059846 35.0 34.0 36.0 31.0 39.0 80-81 34.50139419963311 35.0 34.0 36.0 31.0 37.5 82-83 34.22636812902768 35.0 34.0 35.5 31.0 37.0 84-85 33.94443303185121 35.0 34.0 35.0 31.0 36.5 86-87 33.99755673359614 35.0 34.0 35.0 31.0 36.0 88-89 33.839328686920126 35.0 34.0 35.0 31.0 36.0 90-91 33.77071687382971 35.0 34.0 35.0 31.0 36.0 92-93 33.70463065335478 35.0 34.0 35.0 31.0 35.5 94-95 33.75097750924671 35.0 34.0 35.0 31.0 35.0 96-97 33.637703056899696 35.0 34.0 35.0 31.0 35.0 98-99 33.69473786415368 35.0 33.5 35.0 31.0 35.0 100-101 33.203682657785116 34.5 32.5 35.0 29.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 0.0 23 7.0 24 5.0 25 4.0 26 11.0 27 10.0 28 18.0 29 30.0 30 35.0 31 40.0 32 67.0 33 83.0 34 187.0 35 503.0 36 647.0 37 1297.0 38 933.0 39 120.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.0 47.699999999999996 19.525000000000002 17.775 2 17.65 23.225 24.6 34.525 3 15.875 25.775 43.175000000000004 15.174999999999999 4 16.125 23.65 43.9 16.325 5 18.24579039959789 23.272178939432017 42.95049007288264 15.53154058808746 6 17.0 22.95 43.55 16.5 7 18.099999999999998 23.775 42.925000000000004 15.2 8 15.775 23.200000000000003 44.525 16.5 9 15.25 24.3 43.675000000000004 16.775000000000002 10-11 16.3875 23.6375 43.125 16.85 12-13 16.8625 23.4375 43.512499999999996 16.1875 14-15 16.412499999999998 24.3 42.699999999999996 16.5875 16-17 16.825000000000003 24.099999999999998 42.762499999999996 16.3125 18-19 15.7625 24.25 43.237500000000004 16.75 20-21 16.725 24.425 42.912499999999994 15.937499999999998 22-23 16.7375 23.5875 43.2875 16.3875 24-25 17.05 23.7375 43.0375 16.175 26-27 16.3375 24.349999999999998 42.725 16.5875 28-29 16.7875 23.8375 43.1625 16.2125 30-31 17.622430855112917 24.816036538949504 41.04288251712763 16.518650088809945 32-33 17.48957352347639 26.71868693663393 38.45015471545809 17.34158482443159 34-35 19.100336322869953 27.858744394618835 35.00560538116592 18.03531390134529 36-37 18.598689831956708 28.21133580176588 34.477356878382224 18.712617487895187 38-39 18.942667819072316 28.0466724286949 34.18323249783924 18.827427254393548 40-41 18.758160452633106 29.043957638183667 32.801392717249385 19.396489191933846 42-43 18.720794392523366 29.132593457943923 33.55724299065421 18.589369158878505 44-45 19.307003376890325 28.938481867567173 33.18161797092938 18.57289678461313 46-47 18.63033873343152 28.718703976435933 33.65243004418262 18.998527245949926 48-49 19.589304180824346 29.85669966021569 32.29428275963953 18.259713399320432 50-51 18.92973613993478 30.04743551734361 31.94485621108805 19.07797213163356 52-53 19.179304192685105 30.493606898602437 31.51947665774606 18.8076122509664 54-55 18.635483390436466 29.658870847609116 32.444510650975715 19.261135110978696 56-57 19.091723931879294 30.11652225873917 31.356438601732894 19.43531520764864 58-59 19.127567081397093 30.685054714435616 32.21406086044071 17.973317343726578 60-61 18.78103837471783 30.940556809631303 31.015801354401805 19.262603461249057 62-63 18.63869604587987 30.72743736794446 32.19136734077875 18.44249924539692 64-65 19.05266343825666 30.705205811138015 31.083535108958838 19.15859564164649 66-67 18.255053959568322 32.02614379084967 30.36935704514364 19.349445204438364 68-69 19.221374045801525 32.137404580152676 30.38167938931298 18.259541984732824 70-71 18.562874251497004 32.98019345923537 30.46215261784124 17.99477967142638 72-73 19.31502622647331 32.891082999074364 29.065103363159515 18.72878741129281 74-75 18.997361477572557 34.067980754307 28.853018780071395 18.081638988049047 76-77 17.896707754719927 34.82602590107661 28.35075674832267 18.92650959588079 78-79 19.724025974025974 34.57792207792208 27.305194805194805 18.392857142857146 80-81 19.677966101694917 36.16949152542373 24.71186440677966 19.440677966101696 82-83 19.006147540983605 37.26092896174863 23.3948087431694 20.33811475409836 84-85 20.089516267860215 37.16646582888622 23.618522981580305 19.125494921673265 86-87 19.224108070661586 36.629719431936266 24.541046068583306 19.605126428818842 88-89 19.867549668874172 37.33008016730568 23.631927500871384 19.170442662948766 90-91 19.009866102889358 38.09020436927414 23.185341789992954 19.71458773784355 92-93 18.627450980392158 37.86096256684492 24.046345811051694 19.46524064171123 94-95 19.130434782608695 38.78623188405797 22.717391304347824 19.365942028985508 96-97 19.30856932695844 37.45862449429937 23.317396101507907 19.915410077234277 98-99 19.293325855300054 37.147130304729856 23.481024490558983 20.078519349411106 100-101 19.70917665553154 38.45549223816074 22.34230693652977 19.493024169777954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 152.0 1 311.5 2 552.5 3 604.0 4 496.0 5 211.0 6 5.0 7 5.5 8 5.0 9 6.0 10 7.0 11 8.5 12 9.5 13 8.5 14 9.5 15 9.5 16 6.0 17 7.0 18 7.5 19 7.0 20 16.5 21 22.0 22 19.5 23 22.0 24 25.0 25 23.0 26 23.5 27 23.5 28 28.0 29 35.5 30 37.0 31 38.5 32 46.5 33 53.0 34 61.0 35 68.5 36 71.0 37 98.5 38 113.5 39 116.5 40 129.5 41 140.5 42 138.5 43 126.0 44 117.0 45 120.0 46 113.5 47 91.0 48 88.0 49 93.0 50 77.5 51 74.0 52 77.5 53 69.5 54 59.5 55 48.0 56 42.5 57 33.5 58 34.0 59 34.5 60 30.5 61 27.5 62 23.5 63 23.5 64 24.0 65 22.5 66 18.0 67 14.5 68 17.0 69 11.5 70 7.0 71 10.0 72 12.0 73 9.5 74 7.5 75 5.5 76 3.5 77 4.5 78 3.5 79 2.5 80 3.0 81 3.0 82 2.5 83 2.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.525 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 224.0 32-33 190.0 34-35 63.0 36-37 43.0 38-39 27.0 40-41 24.0 42-43 17.0 44-45 15.0 46-47 9.0 48-49 10.0 50-51 13.0 52-53 7.0 54-55 7.0 56-57 13.0 58-59 14.0 60-61 8.0 62-63 9.0 64-65 13.0 66-67 14.0 68-69 20.0 70-71 16.0 72-73 19.0 74-75 16.0 76-77 24.0 78-79 231.0 80-81 22.0 82-83 23.0 84-85 17.0 86-87 20.0 88-89 23.0 90-91 36.0 92-93 48.0 94-95 34.0 96-97 45.0 98-99 57.0 100-101 2629.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.44999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 98.33224329627207 75.175 2 0.4251144538914323 0.65 3 0.3270111183780248 0.75 4 0.1962066710268149 0.6 5 0.09810333551340746 0.375 6 0.09810333551340746 0.44999999999999996 7 0.06540222367560497 0.35000000000000003 8 0.0 0.0 9 0.0 0.0 >10 0.2616088947024199 3.8249999999999997 >50 0.032701111837802485 1.7500000000000002 >100 0.1635055591890124 16.075 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 193 4.825 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 119 2.9749999999999996 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 2.85 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 2.8000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 105 2.625 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 70 1.7500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.8500000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27 0.675 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.575 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.42500000000000004 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 100 0.0 78.47722 1 CTTTTTT 100 0.0 78.47722 2 >>END_MODULE SRR13172452 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172452_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.56375 34.0 33.0 40.0 31.0 41.0 2 35.831 34.0 34.0 41.0 31.0 41.0 3 35.61925 34.0 34.0 40.0 31.0 41.0 4 37.86175 37.0 37.0 41.0 35.0 41.0 5 37.87475 37.0 37.0 41.0 35.0 41.0 6 37.83 37.0 37.0 41.0 35.0 41.0 7 37.87425 37.0 37.0 41.0 35.0 41.0 8 37.68475 37.0 37.0 41.0 35.0 41.0 9 38.55325 39.0 38.0 41.0 35.0 41.0 10-11 38.679125 39.0 39.0 41.0 36.0 41.0 12-13 38.736999999999995 39.0 39.0 41.0 37.0 41.0 14-15 38.486625000000004 39.5 37.0 41.0 35.5 41.0 16-17 37.902 39.5 35.0 41.0 35.0 41.0 18-19 37.805625 39.0 35.0 41.0 35.0 41.0 20-21 37.65375 39.0 35.0 41.0 35.0 41.0 22-23 37.463 39.0 35.0 41.0 34.5 41.0 24-25 37.429125 39.0 35.0 41.0 34.5 41.0 26-27 37.467625 39.0 35.0 41.0 34.5 41.0 28-29 37.138000000000005 38.0 35.0 41.0 33.0 41.0 30-31 37.26944818652849 38.5 35.0 41.0 33.0 41.0 32-33 37.30480620497735 39.0 35.0 41.0 33.0 41.0 34-35 37.39120578651071 39.0 35.0 41.0 33.0 41.0 36-37 37.33690651479483 39.0 35.0 41.0 33.0 41.0 38-39 37.310301133339294 39.0 35.0 41.0 33.0 41.0 40-41 37.305804433395636 38.5 35.0 41.0 33.0 41.0 42-43 37.030452777030966 38.0 35.0 40.0 33.0 41.0 44-45 36.85342231613501 38.0 35.0 40.0 33.0 41.0 46-47 36.73288491535892 37.5 35.0 40.0 33.0 41.0 48-49 36.77519148913639 37.0 35.0 40.0 33.0 41.0 50-51 36.36605147260468 36.5 35.0 39.5 32.5 40.5 52-53 36.50318930049195 37.0 35.0 40.0 33.0 40.5 54-55 36.64098216388648 36.5 35.0 40.0 33.0 41.0 56-57 36.45160363850577 36.0 35.0 40.0 33.0 41.0 58-59 36.44478793328288 36.0 35.0 40.0 33.0 41.0 60-61 36.21815046087136 35.5 35.0 40.0 32.5 41.0 62-63 36.01731347355929 35.0 35.0 39.5 32.0 41.0 64-65 35.871153068931704 35.0 35.0 39.0 32.0 41.0 66-67 35.651429118048924 35.0 35.0 39.0 32.0 41.0 68-69 35.42266207555747 35.0 34.5 38.0 31.5 41.0 70-71 35.12941873315953 35.0 34.0 37.0 31.0 40.0 72-73 35.0335213349511 35.0 34.0 37.0 31.0 40.0 74-75 34.7777586849469 35.0 34.0 36.5 31.0 39.0 76-77 34.63189080650514 35.0 34.0 36.0 31.0 39.0 78-79 34.41144843962008 35.0 34.0 36.0 30.5 38.5 80-81 34.45958035188289 35.0 34.0 36.0 31.5 37.5 82-83 34.27193861572189 35.0 34.0 36.0 31.0 37.0 84-85 33.93372650537964 35.0 34.0 35.0 30.0 37.0 86-87 33.72819856075992 35.0 34.0 35.0 30.0 36.0 88-89 33.601243427810005 35.0 33.5 35.0 30.0 36.0 90-91 33.52363369558378 35.0 33.0 35.0 30.0 36.0 92-93 33.43386923717098 35.0 33.0 35.0 29.5 35.0 94-95 33.60755452911534 35.0 33.0 35.0 31.0 35.0 96-97 33.548762707761625 35.0 33.0 35.0 30.0 35.0 98-99 33.652367846159706 35.0 33.0 35.0 30.5 35.0 100-101 33.19951183169311 34.5 32.5 35.0 29.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 1.0 21 3.0 22 3.0 23 5.0 24 9.0 25 6.0 26 11.0 27 17.0 28 21.0 29 40.0 30 52.0 31 59.0 32 65.0 33 101.0 34 233.0 35 600.0 36 716.0 37 1278.0 38 695.0 39 82.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.0 62.150000000000006 14.6 13.25 2 12.65 19.325 15.875 52.15 3 11.55 33.6 44.4 10.45 4 12.45 17.424999999999997 57.8 12.325 5 26.85 18.325 42.9 11.924999999999999 6 28.225 17.125 42.275 12.375 7 27.150000000000002 18.45 42.9 11.5 8 19.45 20.9 45.925 13.725000000000001 9 16.375 23.1 46.075 14.45 10-11 17.025000000000002 21.512500000000003 47.0125 14.45 12-13 15.9 21.5625 47.5 15.037500000000001 14-15 16.425 21.3875 46.949999999999996 15.2375 16-17 16.5625 21.475 46.6 15.3625 18-19 16.35 21.712500000000002 46.9875 14.95 20-21 15.662499999999998 21.712500000000002 47.325 15.299999999999999 22-23 15.475 21.349999999999998 47.925000000000004 15.25 24-25 16.45 21.8875 46.9625 14.7 26-27 16.525000000000002 21.8 46.4375 15.2375 28-29 16.037499999999998 21.85 46.800000000000004 15.312500000000002 30-31 15.928753180661579 23.02798982188295 45.55979643765903 15.48346055979644 32-33 17.52025264314156 23.94617602636276 41.69984896333928 16.833722367156394 34-35 18.5238994624437 25.90440215022519 38.32631120151097 17.245387185820135 36-37 20.044642857142858 25.50595238095238 36.50297619047619 17.946428571428573 38-39 18.442807755899594 27.055463700586202 36.88561551179919 17.616113031715017 40-41 19.033186846491894 26.822245794817395 36.12668586149417 18.017881497196544 42-43 18.337147215865752 28.176964149504197 35.57589626239512 17.909992372234935 44-45 20.537221795855718 26.277820414428245 35.42594013814275 17.75901765157329 46-47 19.20407218880148 28.10427271325004 34.6907296004936 18.00092549745488 48-49 19.112627986348123 27.13310580204778 35.107043127520946 18.64722308408315 50-51 19.663446556559677 27.641009660330322 34.0137114365846 18.681832346525397 52-53 19.78743357299156 28.211941231634885 34.010628321350424 17.98999687402313 54-55 19.117877884162613 29.037827656568826 33.63679171244702 18.207502746821536 56-57 19.57345971563981 28.183254344391784 34.09162717219589 18.15165876777251 58-59 19.38353987925008 29.567842389577375 33.158563711471245 17.890054019701303 60-61 19.51491941917983 29.088878251156853 33.1099409605872 18.28626136907611 62-63 19.55128205128205 28.557692307692307 33.205128205128204 18.685897435897438 64-65 19.98069498069498 30.759330759330762 31.66023166023166 17.599742599742598 66-67 19.195996125282534 30.12592831772683 32.37003551824346 18.308040038747176 68-69 19.523269012485812 29.641640992378793 32.36581806388844 18.469271931246958 70-71 19.35746901500326 32.32224396607958 30.805609915198957 17.5146771037182 72-73 18.974274946747503 31.296083893167292 31.41078158282812 18.318859577257086 74-75 19.07514450867052 31.72584640792733 30.800990916597854 18.398018166804295 76-77 19.22501247297522 32.14701480126393 30.61699650756694 18.010976218193914 78-79 19.63869463869464 34.53768453768454 27.447552447552447 18.37606837606838 80-81 19.728984841525037 37.00045934772623 23.702342673403766 19.56821313734497 82-83 19.109162243249482 36.97207477498269 23.95568889914609 19.96307408262174 84-85 19.893097838717175 37.4854752498257 24.052986288635836 18.568440622821285 86-87 20.4311152764761 37.019681349578256 24.554826616682288 17.994376757263357 88-89 20.311027332704995 37.629594721960416 24.17530631479736 17.88407163053723 90-91 19.55439677648732 36.52524294856601 24.579284190566487 19.341076084380184 92-93 19.96648312185779 39.07110366291597 22.695714627723245 18.26669858750299 94-95 20.217917675544793 38.256658595641646 23.07506053268765 18.450363196125906 96-97 18.222112780103423 37.79857178034967 24.402856439300667 19.576459000246242 98-99 19.078617926890335 38.50776164246369 24.13620430645969 18.27741612418628 100-101 18.869908015768726 39.053876478318 23.390275952693823 18.68593955321945 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 134.0 1 354.5 2 696.5 3 765.5 4 622.5 5 268.5 6 6.0 7 6.5 8 6.5 9 8.0 10 6.0 11 4.0 12 4.5 13 6.5 14 7.0 15 7.0 16 8.0 17 7.0 18 7.0 19 8.5 20 10.5 21 12.0 22 12.5 23 16.5 24 17.0 25 14.0 26 17.0 27 20.0 28 21.5 29 23.0 30 28.5 31 33.0 32 38.0 33 40.5 34 54.0 35 70.5 36 70.0 37 84.0 38 101.5 39 113.0 40 129.5 41 133.5 42 142.0 43 133.5 44 121.5 45 126.0 46 111.5 47 106.0 48 104.5 49 98.0 50 100.5 51 83.0 52 71.5 53 70.5 54 60.5 55 59.0 56 56.5 57 50.5 58 42.0 59 37.5 60 35.0 61 30.5 62 27.5 63 25.0 64 21.0 65 20.5 66 19.0 67 15.5 68 15.5 69 13.5 70 14.5 71 14.5 72 12.5 73 12.5 74 7.5 75 4.5 76 4.0 77 2.5 78 1.5 79 2.0 80 3.5 81 2.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 288.0 32-33 240.0 34-35 103.0 36-37 34.0 38-39 28.0 40-41 24.0 42-43 19.0 44-45 19.0 46-47 14.0 48-49 19.0 50-51 10.0 52-53 12.0 54-55 19.0 56-57 22.0 58-59 10.0 60-61 15.0 62-63 12.0 64-65 13.0 66-67 10.0 68-69 20.0 70-71 12.0 72-73 26.0 74-75 18.0 76-77 65.0 78-79 766.0 80-81 12.0 82-83 14.0 84-85 17.0 86-87 14.0 88-89 10.0 90-91 20.0 92-93 21.0 94-95 36.0 96-97 32.0 98-99 44.0 100-101 1962.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.075 #Duplication Level Percentage of deduplicated Percentage of total 1 98.13577207175518 69.75 2 0.28139289482940555 0.4 3 0.38691523039043263 0.8250000000000001 4 0.17587055926837847 0.5 5 0.1055223355610271 0.375 6 0.1055223355610271 0.44999999999999996 7 0.1055223355610271 0.525 8 0.14069644741470277 0.8 9 0.1055223355610271 0.675 >10 0.2110446711220542 2.675 >50 0.07034822370735139 4.0 >100 0.17587055926837847 19.025 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 222 5.55 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 3.6999999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 3.5000000000000004 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 3.4750000000000005 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 2.8000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 2.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 1.525 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.975 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0125 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTTT 115 0.0 73.65 2 CATGGGG 15 0.0016885214 73.649994 2 ACTTTTT 120 0.0 70.581245 1 ACATGGG 60 0.0 67.5125 1 CATGGGA 25 1.6438645E-4 58.920002 2 >>END_MODULE Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396979 spots for SRR13172452.sra Written 1396979 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra Read 1396971 spots for SRR13172452.sra Written 1396971 spots for SRR13172452.sra SRR ids: ['SRR13172452.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__sop3jxd SRR13172452.sra spots: 27939428 blocks: [[1, 1396971], [1396972, 2793942], [2793943, 4190913], [4190914, 5587884], [5587885, 6984855], [6984856, 8381826], [8381827, 9778797], [9778798, 11175768], [11175769, 12572739], [12572740, 13969710], [13969711, 15366681], [15366682, 16763652], [16763653, 18160623], [18160624, 19557594], [19557595, 20954565], [20954566, 22351536], [22351537, 23748507], [23748508, 25145478], [25145479, 26542449], [26542450, 27939428]] SRR13172452 file size 5948111 SRR13172452 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172452 SRR13172452_1.fastq SRR13172452_2.fastq Input file: SRR13172452_1.fastq Paired file: SRR13172452_2.fastq trimmed: SRR13172452-trimmed-pair1.fastq, SRR13172452-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:47:38 2024 >> started Fri Dec 6 10:48:05 2024 >> done (26.137s) 27939428 read pairs processed; of these: 30 ( 0.00%) short read pairs filtered out after trimming by size control 418 ( 0.00%) empty read pairs filtered out after trimming by size control 27938980 (100.00%) read pairs available; of these: 1135538 ( 4.06%) trimmed read pairs available after processing 26803442 (95.94%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 2 0.00% 20 6 0.00% 21 2 0.00% 22 5 0.00% 23 5 0.00% 24 4 0.00% 25 4 0.00% 26 5 0.00% 27 2 0.00% 28 8 0.00% 29 297 0.00% 30 2010 0.01% 31 4098 0.01% 32 5154 0.02% 33 5898 0.02% 34 6298 0.02% 35 6664 0.02% 36 7201 0.03% 37 7758 0.03% 38 8680 0.03% 39 9538 0.03% 40 10236 0.04% 41 10739 0.04% 42 12178 0.04% 43 13433 0.05% 44 15213 0.05% 45 17157 0.06% 46 19546 0.07% 47 22242 0.08% 48 25441 0.09% 49 29177 0.10% 50 33332 0.12% 51 38537 0.14% 52 43448 0.16% 53 49167 0.18% 54 69775 0.25% 55 76282 0.27% 56 80698 0.29% 57 85650 0.31% 58 95575 0.34% 59 105400 0.38% 60 118594 0.42% 61 130752 0.47% 62 158973 0.57% 63 222295 0.80% 64 299536 1.07% 65 1444402 5.17% 66 2452705 8.78% 67 1368698 4.90% 68 579699 2.07% 69 331926 1.19% 70 265356 0.95% 71 246618 0.88% 72 233456 0.84% 73 223584 0.80% 74 230424 0.82% 75 214347 0.77% 76 209563 0.75% 77 222564 0.80% 78 303978 1.09% 79 271204 0.97% 80 259672 0.93% 81 245297 0.88% 82 276072 0.99% 83 292378 1.05% 84 311080 1.11% 85 317219 1.14% 86 381214 1.36% 87 431676 1.55% 88 614392 2.20% 89 5759684 20.62% 90 215380 0.77% 91 60843 0.22% 92 51661 0.18% 93 53648 0.19% 94 63366 0.23% 95 78321 0.28% 96 97679 0.35% 97 148600 0.53% 98 378764 1.36% 99 502679 1.80% 100 1518793 5.44% 101 5435017 19.45% 27938980 reads passed initial QC criterion=sequence-density sequence-density=0.09 sequence-density-rank=1 fanout-score=8.05 fanout-score-rank=15 prefix-density=0.22 prefix-fanout=3.2 sequence=GCTAGCTAGCTGGGCGGCGATGGTGGGTGCATGCTTGCAGTGCAGTTGTCCTAGATCCTGGATCGATCCTCATTCCTCATGGTCGCTGGTGTGTGGCTCTAGTTGCAGGTGCAGCAGGAGCAGCCGCAGGCGGTGCCGCACTTG criterion=fanout-score sequence-density=0.04 sequence-density-rank=15 fanout-score=124.02 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=19.5 sequence=AAGAAGAAGAAA criterion=sequence-density sequence-density=0.13 sequence-density-rank=1 fanout-score=2.47 fanout-score-rank=14 prefix-density=0.04 prefix-fanout=2.5 sequence=ACATGGGGAAGA criterion=fanout-score sequence-density=0.04 sequence-density-rank=37 fanout-score=38.77 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=12.9 sequence=TTTTTCTTCTTCTTT SRR13172452 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:48:52 Started mapping on | Dec 06 10:48:52 Finished on | Dec 06 10:58:40 Mapping speed, Million of reads per hour | 171.05 Number of input reads | 27938980 Average input read length | 167 UNIQUE READS: Uniquely mapped reads number | 16725999 Uniquely mapped reads % | 59.87% Average mapped length | 164.55 Number of splices: Total | 4265175 Number of splices: Annotated (sjdb) | 3820480 Number of splices: GT/AG | 4136752 Number of splices: GC/AG | 56393 Number of splices: AT/AC | 3024 Number of splices: Non-canonical | 69006 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.54 Insertion rate per base | 0.01% Insertion average length | 1.72 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1825170 % of reads mapped to multiple loci | 6.53% Number of reads mapped to too many loci | 92729 % of reads mapped to too many loci | 0.33% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 32.92% % of reads unmapped: other | 0.35% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 13661851 13661851 13661851 N_multimapping 1825170 1825170 1825170 N_noFeature 666247 9804118 7176326 N_ambiguous 485747 36087 43614 UnstrandedReadsAssigned:15574005 PositiveStrandReadsAssigned:6885794 NegativeStrandReadsAssigned:9506059 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=78 echo kmer=73 SRR13172452 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172452-trimmed-pair1.fastq SRR13172452-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,938,980 reads, 21,032,031 reads pseudoaligned [quant] estimated average fragment length: 152.11 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,127 rounds 52973 SRR13172452.ke.tsv 35125 SRR13172452.se.tsv 88098 total ==> SRR13172452.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 785.005 0 0 PNS24247 1044 892.89 0 0 PNS24249 1928 1776.89 0 0 PNS24246 1044 892.89 0 0 PNS24248 1044 892.89 0 0 PNS24244 1471 1319.89 523 28.6202 PNS24243 293 144.249 0 0 KQK14069 1603 1451.89 68 3.38285 KQK14071 474 323.82 0 0 ==> SRR13172452.se.tsv <== BRADI_1g14170v3 63 BRADI_1g53295v3 105 BRADI_1g59795v3 370 BRADI_1g07683v3 0 BRADI_1g00485v3 195 BRADI_1g20270v3 1129 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 184 BRADI_1g48960v3 0 SRR13172452 completed mapping pipeline successfully