Starting /dee2/code/volunteer_pipeline.sh SRR13172453 current disk space = 1551381311488 free memory = 1597399756 SRR13172453 SRAfilesize ea0b6b40956f4e3ca91b88db9deb140d SRR13172453.sra SRR13172453.sra file validated SRR13172453 is paired end SRR13172453 is conventional basespace SRR13172453 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172453_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.22775 34.0 33.0 34.0 31.0 40.0 2 34.65375 34.0 34.0 34.0 31.0 41.0 3 34.64525 34.0 34.0 34.0 31.0 41.0 4 37.2745 37.0 37.0 37.0 35.0 41.0 5 37.1975 37.0 37.0 37.0 35.0 41.0 6 37.27975 37.0 37.0 37.0 35.0 41.0 7 37.25525 37.0 37.0 37.0 35.0 41.0 8 37.3435 37.0 37.0 37.0 35.0 41.0 9 38.79075 39.0 39.0 39.0 37.0 41.0 10-11 38.765625 39.0 39.0 39.0 37.0 41.0 12-13 38.75 39.0 39.0 39.0 37.0 41.0 14-15 39.204499999999996 40.0 38.0 41.0 36.5 41.0 16-17 38.749624999999995 40.0 36.5 41.0 35.0 41.0 18-19 38.770375 40.0 35.5 41.0 35.0 41.0 20-21 38.688375 40.0 35.0 41.0 35.0 41.0 22-23 38.589625 40.0 35.0 41.0 35.0 41.0 24-25 38.428 40.0 35.0 41.0 35.0 41.0 26-27 38.422250000000005 40.0 35.0 41.0 35.0 41.0 28-29 38.392875000000004 40.0 35.0 41.0 35.0 41.0 30-31 38.28347755417957 40.0 35.0 41.0 35.0 41.0 32-33 38.396159519029894 40.0 36.5 41.0 35.0 41.0 34-35 38.51478223091989 40.0 37.0 41.0 35.0 41.0 36-37 38.58259281177914 40.0 37.5 41.0 35.0 41.0 38-39 38.45533481314841 40.0 37.0 41.0 35.0 41.0 40-41 38.43301673017235 40.0 37.0 41.0 35.0 41.0 42-43 38.28704144489686 40.0 37.0 41.0 35.0 41.0 44-45 38.21687115721669 40.0 36.0 41.0 34.5 41.0 46-47 38.14281459375143 40.0 36.0 41.0 34.5 41.0 48-49 37.961082157491894 40.0 35.0 41.0 34.0 41.0 50-51 37.90890942399984 40.0 35.0 41.0 34.0 41.0 52-53 37.779638975085945 39.0 35.0 41.0 33.5 41.0 54-55 37.66629172341294 39.0 35.0 41.0 33.5 41.0 56-57 37.47077768433154 39.0 35.0 41.0 33.0 41.0 58-59 37.48515740502988 39.0 35.0 40.5 33.5 41.0 60-61 37.20442243864467 38.0 35.0 40.0 33.0 41.0 62-63 36.80512054150827 37.5 35.0 40.0 32.5 41.0 64-65 36.59216568574607 37.0 35.0 40.0 33.0 41.0 66-67 36.34042881134202 36.5 35.0 39.5 33.0 41.0 68-69 36.050561921368256 36.0 35.0 39.0 32.5 41.0 70-71 35.67447205673905 35.0 34.5 39.0 31.5 40.5 72-73 35.37635386657411 35.0 34.5 38.0 31.5 40.0 74-75 34.80348914339616 35.0 34.0 37.0 30.5 39.5 76-77 33.83820532536928 34.5 32.5 36.0 29.5 38.5 78-79 34.53059696367367 35.0 34.0 36.0 31.0 39.0 80-81 34.60480459261605 35.0 34.0 36.0 31.5 37.5 82-83 34.35481296109321 35.0 34.0 35.5 31.0 37.0 84-85 34.1907684541267 35.0 34.0 35.0 31.0 37.0 86-87 34.13864460544503 35.0 34.0 35.0 31.0 36.0 88-89 34.0322630256309 35.0 34.0 35.0 31.0 36.0 90-91 33.78115385358289 35.0 34.0 35.0 31.0 36.0 92-93 33.60235970593478 35.0 33.5 35.0 30.5 36.0 94-95 33.492219173808365 35.0 33.0 35.0 30.5 35.0 96-97 33.513340712402105 35.0 33.0 35.0 30.0 35.0 98-99 33.27921379001675 35.0 33.0 35.0 29.5 35.0 100-101 33.24473296599397 34.5 32.0 35.0 30.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 1.0 24 1.0 25 8.0 26 9.0 27 18.0 28 12.0 29 23.0 30 30.0 31 39.0 32 68.0 33 118.0 34 181.0 35 419.0 36 654.0 37 1318.0 38 960.0 39 139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.7 44.625 19.825 19.85 2 18.675 23.775 23.150000000000002 34.4 3 18.35 24.575 40.225 16.85 4 17.1 22.975 42.475 17.45 5 18.883580588383204 21.47347246668343 40.558209705808395 19.08473723912497 6 19.0 21.7 41.349999999999994 17.95 7 20.1 21.925 40.875 17.1 8 18.375 22.925 40.425 18.275 9 17.525 22.25 42.699999999999996 17.525 10-11 18.862499999999997 22.375 40.275 18.4875 12-13 18.075 23.5625 40.4625 17.9 14-15 18.2 23.6875 40.150000000000006 17.962500000000002 16-17 18.1625 22.7375 41.0625 18.0375 18-19 18.1125 22.75 40.5 18.637500000000003 20-21 17.8125 23.1875 40.6625 18.337500000000002 22-23 17.974999999999998 23.575 39.887499999999996 18.5625 24-25 17.75 22.8125 41.2625 18.175 26-27 17.8125 22.775000000000002 41.75 17.6625 28-29 18.5 23.025000000000002 41.2125 17.2625 30-31 19.019807008633823 22.892331132554595 40.00761808024378 18.0802437785678 32-33 19.173642992168514 24.520658925195786 36.30839859573319 19.997299486902513 34-35 19.57164999295477 26.8141468226011 33.46484430040862 20.14935888403551 36-37 20.639243227748317 26.745019349290526 31.446180306722088 21.169557116239073 38-39 21.187662150475642 27.082732776016144 31.565292591524933 20.16431248198328 40-41 21.041183294663572 27.01566125290023 31.25 20.693155452436194 42-43 20.232896652110625 28.078602620087334 30.68413391557496 21.004366812227072 44-45 22.11833455076698 26.64718772826881 31.102994886778674 20.131482834185537 46-47 20.925464928979352 27.353931761604922 30.780494948015814 20.94010836139991 48-49 21.059583211036102 28.1919577340769 30.3492808922806 20.3991781626064 50-51 20.541494997057093 27.516185991759855 31.135962330782814 20.806356680400235 52-53 21.325265643447462 28.246753246753247 30.15053128689492 20.277449822904366 54-55 21.6580310880829 28.068097705403407 30.03700962250185 20.23686158401184 56-57 21.05106888361045 27.850356294536816 30.28503562945368 20.81353919239905 58-59 21.803839857121595 27.81663938086025 29.126358089001343 21.253162673016817 60-61 21.074626865671643 28.223880597014926 30.492537313432837 20.208955223880597 62-63 20.991166342266805 28.74681838598593 29.720017966761493 20.541997304985777 64-65 20.330082520630157 28.657164291072768 30.202550637659414 20.810202550637662 66-67 21.49842034000301 28.117947946442 29.366631563111177 21.01700015044381 68-69 20.924610968424233 29.85345218310923 28.9167547967971 20.305182051669437 70-71 20.84848484848485 29.954545454545457 28.530303030303028 20.666666666666668 72-73 20.395136778115504 30.37993920972644 28.632218844984802 20.592705167173253 74-75 20.497937986864212 29.799908354971745 29.112570643042613 20.58958301512143 76-77 20.745170193192273 30.512112848819378 27.782888684452622 20.959828273535724 78-79 20.788812067881835 30.95537397862979 27.199874292897547 21.055939660590823 80-81 22.211429496519347 31.843937186336408 25.31973449894771 20.624898818196534 82-83 21.889250814332247 32.65472312703583 24.98371335504886 20.47231270358306 84-85 21.56158118262006 31.836001306762498 25.220516171185885 21.381901339431558 86-87 21.87192118226601 32.9064039408867 25.336617405582924 19.885057471264368 88-89 21.02657204159102 32.21653738240634 25.466248555867306 21.290642020135337 90-91 20.681063122923586 33.23920265780731 24.269102990033222 21.81063122923588 92-93 21.268030862126803 33.10969473331097 25.159342502515937 20.462931902046293 94-95 21.38620572784274 32.7910523640061 24.131503135061855 21.691238773089307 96-97 20.76053442959918 32.95649194929771 25.23124357656732 21.051730044535798 98-99 20.08324661810614 33.905653832813044 25.89316684009712 20.117932708983695 100-101 20.459136822773186 33.02112029384757 24.187327823691458 22.332415059687786 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 83.0 1 227.0 2 497.0 3 594.5 4 494.0 5 212.5 6 3.5 7 5.0 8 5.5 9 4.5 10 4.5 11 4.5 12 5.0 13 5.0 14 3.0 15 3.0 16 7.5 17 10.5 18 9.5 19 10.0 20 10.5 21 9.0 22 8.5 23 9.0 24 16.5 25 19.0 26 15.5 27 20.5 28 23.0 29 21.0 30 21.0 31 29.0 32 42.0 33 49.0 34 56.0 35 70.0 36 72.0 37 79.5 38 100.0 39 122.0 40 140.5 41 151.0 42 150.0 43 135.5 44 134.5 45 126.0 46 107.0 47 102.5 48 99.5 49 105.5 50 106.0 51 86.0 52 73.0 53 71.0 54 74.0 55 65.5 56 54.0 57 57.5 58 48.5 59 35.5 60 37.0 61 40.5 62 40.0 63 33.5 64 28.5 65 23.0 66 21.5 67 23.0 68 22.0 69 23.5 70 22.5 71 21.5 72 18.5 73 13.5 74 9.5 75 6.5 76 8.0 77 7.0 78 3.0 79 1.5 80 0.5 81 1.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.575 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.02920987293705272 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 240.0 32-33 180.0 34-35 84.0 36-37 22.0 38-39 24.0 40-41 11.0 42-43 13.0 44-45 8.0 46-47 9.0 48-49 10.0 50-51 10.0 52-53 10.0 54-55 9.0 56-57 8.0 58-59 10.0 60-61 11.0 62-63 7.0 64-65 9.0 66-67 12.0 68-69 11.0 70-71 9.0 72-73 15.0 74-75 15.0 76-77 8.0 78-79 160.0 80-81 21.0 82-83 12.0 84-85 14.0 86-87 12.0 88-89 21.0 90-91 21.0 92-93 40.0 94-95 27.0 96-97 31.0 98-99 94.0 100-101 2802.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.875 #Duplication Level Percentage of deduplicated Percentage of total 1 98.4037558685446 78.60000000000001 2 0.5633802816901409 0.8999999999999999 3 0.1564945226917058 0.375 4 0.3129890453834116 1.0 5 0.09389671361502347 0.375 6 0.06259780907668232 0.3 7 0.06259780907668232 0.35000000000000003 8 0.0 0.0 9 0.03129890453834116 0.22499999999999998 >10 0.09389671361502347 1.15 >50 0.09389671361502347 4.95 >100 0.12519561815336464 11.774999999999999 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 122 3.05 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 122 3.05 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 2.875 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 2.8000000000000003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 1.725 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66 1.6500000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 1.575 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21 0.525 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0125 0.0 0.0 0.0 0.0 76-77 0.037500000000000006 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.0875 0.0 0.0 0.0 0.0 88-89 0.1125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 75 0.0 84.8481 1 CTTTTTT 75 0.0 84.8481 2 >>END_MODULE SRR13172453 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172453_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.5395 34.0 33.0 40.0 31.0 41.0 2 35.89175 34.0 34.0 41.0 31.0 41.0 3 35.82775 34.0 34.0 40.0 31.0 41.0 4 37.972 37.0 37.0 41.0 35.0 41.0 5 37.9315 37.0 37.0 41.0 35.0 41.0 6 37.98775 37.0 37.0 41.0 35.0 41.0 7 37.93975 37.0 37.0 41.0 35.0 41.0 8 37.69575 37.0 37.0 41.0 35.0 41.0 9 38.7185 39.0 39.0 41.0 37.0 41.0 10-11 38.724375 39.0 39.0 41.0 37.0 41.0 12-13 38.720625 39.0 39.0 41.0 37.0 41.0 14-15 38.634249999999994 40.0 37.0 41.0 36.0 41.0 16-17 38.1105 40.0 35.0 41.0 35.0 41.0 18-19 37.979 40.0 35.0 41.0 35.0 41.0 20-21 37.978375 40.0 35.0 41.0 35.0 41.0 22-23 37.771875 39.5 35.0 41.0 35.0 41.0 24-25 37.608125 39.0 35.0 41.0 35.0 41.0 26-27 37.736125 39.0 35.0 41.0 35.0 41.0 28-29 37.510374999999996 39.0 35.0 41.0 33.5 41.0 30-31 37.496793274244006 39.0 35.0 41.0 33.0 41.0 32-33 37.624098335519705 39.5 35.0 41.0 33.0 41.0 34-35 37.737975308174654 40.0 35.0 41.0 33.0 41.0 36-37 37.627739012369055 39.0 35.0 41.0 33.0 41.0 38-39 37.68242372959474 39.0 35.0 41.0 33.5 41.0 40-41 37.47200097441845 39.0 35.0 41.0 33.0 41.0 42-43 37.24997442970705 39.0 35.0 41.0 33.0 41.0 44-45 37.1887864364749 38.5 35.0 40.0 33.0 41.0 46-47 36.80607821690562 38.0 35.0 40.0 32.5 41.0 48-49 36.9606066814771 38.0 35.0 40.0 33.0 41.0 50-51 36.523306356373226 37.5 35.0 39.5 32.5 40.5 52-53 36.71543814125507 37.5 35.0 40.0 33.0 40.5 54-55 36.92107806794135 37.0 35.0 40.0 33.0 41.0 56-57 36.89395861412429 37.0 35.0 40.5 33.0 41.0 58-59 36.773026857952445 37.0 35.0 40.0 33.0 41.0 60-61 36.53693294809936 36.0 35.0 40.0 33.0 41.0 62-63 36.236471007045125 36.0 35.0 40.0 33.0 41.0 64-65 36.0174849564818 35.0 35.0 39.5 32.0 41.0 66-67 35.90968555952294 35.0 35.0 39.0 32.0 41.0 68-69 35.55676631855003 35.0 35.0 39.0 31.5 41.0 70-71 35.337691369747404 35.0 34.5 37.5 31.5 40.0 72-73 35.108013359015864 35.0 34.0 37.0 31.0 39.5 74-75 34.95458405765413 35.0 34.0 37.0 31.0 39.0 76-77 34.73165972043738 35.0 34.0 36.0 31.0 39.0 78-79 34.38594740519363 35.0 34.0 36.0 30.5 39.0 80-81 34.39943352392305 35.0 34.0 36.0 31.0 37.5 82-83 34.22372488528175 35.0 34.0 35.5 31.0 37.0 84-85 34.031592004229026 35.0 34.0 35.0 31.0 37.0 86-87 33.79074720839532 35.0 34.0 35.0 30.5 36.0 88-89 33.532485833577454 35.0 33.5 35.0 29.5 36.0 90-91 33.4903326123309 35.0 33.5 35.0 29.5 36.0 92-93 33.508295330611006 35.0 33.0 35.0 30.0 35.5 94-95 33.55789372644825 35.0 34.0 35.0 30.0 35.0 96-97 33.54040826605318 35.0 33.0 35.0 30.0 35.0 98-99 33.609771071759056 35.0 33.0 35.0 30.5 35.0 100-101 33.16121468705968 34.5 32.0 35.0 28.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 0.0 22 4.0 23 7.0 24 7.0 25 9.0 26 16.0 27 10.0 28 19.0 29 34.0 30 44.0 31 43.0 32 65.0 33 123.0 34 197.0 35 467.0 36 730.0 37 1405.0 38 728.0 39 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.425 59.95 15.35 13.275 2 14.95 19.225 16.125 49.7 3 12.975 33.300000000000004 40.8 12.925 4 13.350000000000001 18.099999999999998 56.175 12.375 5 30.3 17.25 40.75 11.700000000000001 6 29.025000000000002 16.975 41.05 12.950000000000001 7 28.775000000000002 17.549999999999997 41.0 12.675 8 20.225 19.825 44.0 15.950000000000001 9 18.224999999999998 21.349999999999998 44.925 15.5 10-11 17.349999999999998 21.3875 44.6625 16.6 12-13 16.7625 20.925 45.300000000000004 17.0125 14-15 18.862499999999997 20.5125 44.2 16.425 16-17 17.05 20.9875 44.425 17.5375 18-19 17.7875 21.0125 44.425 16.775000000000002 20-21 17.5875 20.4375 44.587500000000006 17.3875 22-23 18.0 20.424999999999997 45.0125 16.5625 24-25 17.4375 21.3125 44.45 16.8 26-27 17.962500000000002 20.7625 44.487500000000004 16.7875 28-29 17.7625 21.1625 44.75 16.325 30-31 17.76416539050536 22.524247064828995 43.70852475752935 16.003062787136294 32-33 18.55570839064649 22.764786795048142 40.01375515818432 18.665749656121044 34-35 20.54014598540146 25.18248175182482 35.43065693430657 18.846715328467152 36-37 21.244377811094452 24.782608695652176 33.823088455772115 20.14992503748126 38-39 20.533818622990594 26.038823172581132 33.33333333333333 20.094024871094934 40-41 20.79646017699115 25.984131827891368 32.25511138236192 20.96429661275557 42-43 21.44611605772183 26.52747927540682 32.37642001842186 19.649984648449493 44-45 21.771331584632 26.07622280512267 31.67721030705138 20.47523530319395 46-47 21.286821705426355 27.007751937984498 32.10852713178294 19.5968992248062 48-49 20.630954240199905 27.69014524441668 31.219740746525066 20.45915976885835 50-51 21.504869619855484 27.12849513038014 30.89852340559221 20.468111844172164 52-53 21.488254769036732 26.81696358190131 30.994797414472647 20.699984234589312 54-55 21.607086365074345 26.652957924707373 30.797216070863655 20.942739639354635 56-57 21.181327405525565 27.310257224515723 29.78723404255319 21.721181327405525 58-59 21.36030582988213 28.161834979292767 29.229053838802166 21.248805352022938 60-61 21.08203000319183 27.178423236514522 29.95531439514842 21.78423236514523 62-63 22.17419148254883 26.945244956772335 29.731027857829012 21.14953570284982 64-65 21.246185964348804 28.087361490284245 29.725389433113858 20.94106311225309 66-67 22.089648500483715 27.329893582715254 30.748145759432443 19.83231215736859 68-69 21.398510844933636 28.148268047911944 29.89640660407899 20.55681450307543 70-71 21.79987004548408 27.696556205328136 29.0448343079922 21.458739441195583 72-73 21.87908496732026 29.395424836601308 29.46078431372549 19.264705882352942 74-75 22.04309919394637 27.78417502878763 29.560783023523605 20.61194275374239 76-77 21.984758117958915 27.99867461895295 28.959575878064943 21.056991385023196 78-79 21.612903225806452 30.474383301707782 27.855787476280835 20.05692599620493 80-81 21.764834683599737 32.2969126341143 25.049266476899497 20.888986205386466 82-83 21.886709279259424 31.298214679303506 25.060612739695838 21.75446330174124 84-85 22.276243093922652 33.10497237569061 23.734806629834253 20.883977900552487 86-87 21.788329265586864 32.238739738185046 24.872420678943865 21.100510317284225 88-89 21.4 32.644444444444446 25.0 20.955555555555556 90-91 22.329012069736255 32.342422887796154 25.614662494412162 19.713902548055433 92-93 21.666666666666668 32.45495495495496 24.684684684684687 21.193693693693692 94-95 21.149477510222624 32.46251703771013 25.124943207632892 21.263062244434348 96-97 21.639042357274402 33.12615101289134 25.06906077348066 20.165745856353592 98-99 21.677993906726037 33.84110616358097 24.27935317553316 20.201546754159832 100-101 21.094330279772223 33.49839069076504 24.733845011141373 20.673434018321366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 75.0 1 269.5 2 646.5 3 784.5 4 642.5 5 276.5 6 6.5 7 5.5 8 5.0 9 5.0 10 5.5 11 5.5 12 4.0 13 2.5 14 2.5 15 4.0 16 4.0 17 4.0 18 4.0 19 1.5 20 4.5 21 7.5 22 9.0 23 8.5 24 8.0 25 10.0 26 10.5 27 12.0 28 13.5 29 18.0 30 22.5 31 24.5 32 31.0 33 33.5 34 39.0 35 60.5 36 79.0 37 82.5 38 85.0 39 114.0 40 126.5 41 120.5 42 133.0 43 125.5 44 116.5 45 134.0 46 138.0 47 132.5 48 126.5 49 110.5 50 103.5 51 92.0 52 95.5 53 96.5 54 81.5 55 67.5 56 53.0 57 45.5 58 45.0 59 47.5 60 44.0 61 38.0 62 31.5 63 29.5 64 32.5 65 34.5 66 27.5 67 28.0 68 32.5 69 25.5 70 17.5 71 17.5 72 16.0 73 13.5 74 15.0 75 11.5 76 6.0 77 3.5 78 2.0 79 2.0 80 2.0 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 285.0 32-33 256.0 34-35 110.0 36-37 46.0 38-39 22.0 40-41 18.0 42-43 17.0 44-45 18.0 46-47 18.0 48-49 25.0 50-51 10.0 52-53 11.0 54-55 11.0 56-57 13.0 58-59 4.0 60-61 12.0 62-63 8.0 64-65 12.0 66-67 13.0 68-69 9.0 70-71 16.0 72-73 22.0 74-75 21.0 76-77 58.0 78-79 676.0 80-81 18.0 82-83 8.0 84-85 9.0 86-87 3.0 88-89 9.0 90-91 17.0 92-93 20.0 94-95 22.0 96-97 40.0 98-99 46.0 100-101 2097.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.95 #Duplication Level Percentage of deduplicated Percentage of total 1 98.29886591060706 73.675 2 0.46697798532354906 0.7000000000000001 3 0.10006671114076052 0.22499999999999998 4 0.3002001334222815 0.8999999999999999 5 0.20013342228152103 0.75 6 0.06671114076050699 0.3 7 0.06671114076050699 0.35000000000000003 8 0.06671114076050699 0.4 9 0.0 0.0 >10 0.20013342228152103 2.875 >50 0.10006671114076052 5.575 >100 0.13342228152101399 14.249999999999998 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 4.1000000000000005 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 159 3.975 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 129 3.225 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 2.9499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 2.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64 1.6 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63 1.575 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 1.0250000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27 0.675 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.037500000000000006 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.1125 0.0 0.0 0.0 0.0 88-89 0.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 105 0.0 70.4625 1 CATGGGA 25 2.2665627E-6 70.4625 2 ATGGGCG 15 0.0020126011 70.462494 3 ATGGGAA 15 0.0020126011 70.462494 3 CTTTTTT 110 0.0 67.25966 2 ACATGGG 105 0.0 63.75179 1 CATGGGG 35 2.1892811E-7 60.39643 2 CATGGGC 50 4.1585736E-8 49.32375 2 >>END_MODULE Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra Read 1430568 spots for SRR13172453.sra Written 1430568 spots for SRR13172453.sra Read 1430555 spots for SRR13172453.sra Written 1430555 spots for SRR13172453.sra SRR ids: ['SRR13172453.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0m484iia SRR13172453.sra spots: 28611113 blocks: [[1, 1430555], [1430556, 2861110], [2861111, 4291665], [4291666, 5722220], [5722221, 7152775], [7152776, 8583330], [8583331, 10013885], [10013886, 11444440], [11444441, 12874995], [12874996, 14305550], [14305551, 15736105], [15736106, 17166660], [17166661, 18597215], [18597216, 20027770], [20027771, 21458325], [21458326, 22888880], [22888881, 24319435], [24319436, 25749990], [25749991, 27180545], [27180546, 28611113]] SRR13172453 file size 6150718 SRR13172453 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172453 SRR13172453_1.fastq SRR13172453_2.fastq Input file: SRR13172453_1.fastq Paired file: SRR13172453_2.fastq trimmed: SRR13172453-trimmed-pair1.fastq, SRR13172453-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:52:15 2024 >> started Fri Dec 6 10:52:40 2024 >> done (24.334s) 28611113 read pairs processed; of these: 1384 ( 0.00%) short read pairs filtered out after trimming by size control 1879 ( 0.01%) empty read pairs filtered out after trimming by size control 28607850 (99.99%) read pairs available; of these: 709954 ( 2.48%) trimmed read pairs available after processing 27897896 (97.52%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 57 0.00% 19 128 0.00% 20 60 0.00% 21 92 0.00% 22 49 0.00% 23 93 0.00% 24 36 0.00% 25 81 0.00% 26 31 0.00% 27 63 0.00% 28 30 0.00% 29 249 0.00% 30 2170 0.01% 31 4780 0.02% 32 5780 0.02% 33 6736 0.02% 34 7072 0.02% 35 7582 0.03% 36 7937 0.03% 37 8431 0.03% 38 9135 0.03% 39 10139 0.04% 40 10334 0.04% 41 10592 0.04% 42 11438 0.04% 43 12398 0.04% 44 13777 0.05% 45 15482 0.05% 46 17120 0.06% 47 19304 0.07% 48 21884 0.08% 49 24010 0.08% 50 26643 0.09% 51 29689 0.10% 52 33329 0.12% 53 38494 0.13% 54 62165 0.22% 55 65112 0.23% 56 64233 0.22% 57 66587 0.23% 58 72995 0.26% 59 81439 0.28% 60 91827 0.32% 61 104156 0.36% 62 131595 0.46% 63 194693 0.68% 64 293473 1.03% 65 1630570 5.70% 66 2727190 9.53% 67 1454999 5.09% 68 565640 1.98% 69 309400 1.08% 70 240561 0.84% 71 222627 0.78% 72 208263 0.73% 73 198322 0.69% 74 210600 0.74% 75 189157 0.66% 76 180550 0.63% 77 193237 0.68% 78 258769 0.90% 79 224692 0.79% 80 216254 0.76% 81 207336 0.72% 82 231023 0.81% 83 246461 0.86% 84 263256 0.92% 85 276807 0.97% 86 348333 1.22% 87 402080 1.41% 88 690780 2.41% 89 5611260 19.61% 90 205207 0.72% 91 62608 0.22% 92 55005 0.19% 93 58073 0.20% 94 68956 0.24% 95 83178 0.29% 96 106895 0.37% 97 166075 0.58% 98 402690 1.41% 99 576532 2.02% 100 1872574 6.55% 101 6058390 21.18% 28607850 reads passed initial QC criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=2.13 fanout-score-rank=34 prefix-density=0.19 prefix-fanout=2.1 sequence=TGCCGCACTTGCAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=523.19 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=16.3 sequence=GCTGCTGCTTACTAGGTGTGTGTGCCAGCTGTAGGTGTGGAACGGGTGCTGTGCGTGGGTTCGCGGTGACGTGGGCTAAATAAAGTGTTGTCGAGTCGAGCGGGCGGTGAGGCCAGCTAAGCTAGTACGGAGTACTACTACCTGTCAGCGACGCAGTGCCTGTGTCGTTGTTGCAGCTTGTTGTGTGTCGGTCATGTTGCTCATCGAGTTTGTGTTATCTCTGTCAATGGGTTGGGTAATGCGGTTTTCTCACC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=2.85 fanout-score-rank=14 prefix-density=0.33 prefix-fanout=2.6 sequence=AGCTAGCTAGCT criterion=fanout-score sequence-density=0.05 sequence-density-rank=33 fanout-score=85.28 fanout-score-rank=1 prefix-density=0.29 prefix-fanout=15.1 sequence=GCCGCCGCCGCC SRR13172453 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:53:26 Started mapping on | Dec 06 10:53:26 Finished on | Dec 06 11:00:30 Mapping speed, Million of reads per hour | 242.90 Number of input reads | 28607850 Average input read length | 168 UNIQUE READS: Uniquely mapped reads number | 19321046 Uniquely mapped reads % | 67.54% Average mapped length | 164.14 Number of splices: Total | 6012576 Number of splices: Annotated (sjdb) | 5527143 Number of splices: GT/AG | 5850561 Number of splices: GC/AG | 80983 Number of splices: AT/AC | 4018 Number of splices: Non-canonical | 77014 Mismatch rate per base, % | 0.29% Deletion rate per base | 0.02% Deletion average length | 1.49 Insertion rate per base | 0.01% Insertion average length | 1.50 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1751858 % of reads mapped to multiple loci | 6.12% Number of reads mapped to too many loci | 60186 % of reads mapped to too many loci | 0.21% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 25.89% % of reads unmapped: other | 0.24% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 12599145 12599145 12599145 N_multimapping 1751858 1751858 1751858 N_noFeature 687032 11299729 8271015 N_ambiguous 518081 38081 48575 UnstrandedReadsAssigned:18115933 PositiveStrandReadsAssigned:7983236 NegativeStrandReadsAssigned:11001456 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=78 echo kmer=73 SRR13172453 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172453-trimmed-pair1.fastq SRR13172453-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 28,607,850 reads, 21,972,350 reads pseudoaligned [quant] estimated average fragment length: 165.671 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,178 rounds 52973 SRR13172453.ke.tsv 35125 SRR13172453.se.tsv 88098 total ==> SRR13172453.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 771.441 0 0 PNS24247 1044 879.329 0 0 PNS24249 1928 1763.33 104.179 3.82316 PNS24246 1044 879.329 0 0 PNS24248 1044 879.329 0 0 PNS24244 1471 1306.33 428.821 21.2421 PNS24243 293 132.295 4 1.95655 KQK14069 1603 1438.33 231 10.3927 KQK14071 474 310.72 0 0 ==> SRR13172453.se.tsv <== BRADI_1g14170v3 214 BRADI_1g53295v3 0 BRADI_1g59795v3 360 BRADI_1g07683v3 0 BRADI_1g00485v3 471 BRADI_1g20270v3 2754 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 119 BRADI_1g48960v3 0 SRR13172453 completed mapping pipeline successfully