Starting /dee2/code/volunteer_pipeline.sh SRR13172455 current disk space = 1551346122752 free memory = 1596934036 SRR13172455 SRAfilesize 12e09ae8e9d4fd51ca55534ae20f4f15 SRR13172455.sra SRR13172455.sra file validated SRR13172455 is paired end SRR13172455 is conventional basespace SRR13172455 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172455_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.4015 34.0 33.0 34.0 31.0 40.0 2 34.76875 34.0 34.0 34.0 31.0 41.0 3 34.79125 34.0 34.0 34.0 31.0 41.0 4 37.375 37.0 37.0 37.0 35.0 41.0 5 37.2115 37.0 37.0 37.0 35.0 41.0 6 37.304 37.0 37.0 37.0 35.0 41.0 7 37.32825 37.0 37.0 37.0 35.0 41.0 8 37.32325 37.0 37.0 37.0 35.0 41.0 9 38.68125 39.0 39.0 39.0 37.0 41.0 10-11 38.705375000000004 39.0 39.0 39.0 37.0 41.0 12-13 38.696 39.0 39.0 39.0 37.0 41.0 14-15 38.91175 40.0 37.0 41.0 36.5 41.0 16-17 38.354625 40.0 35.0 41.0 35.0 41.0 18-19 38.226625 40.0 35.0 41.0 35.0 41.0 20-21 38.101124999999996 40.0 35.0 41.0 35.0 41.0 22-23 37.853750000000005 39.5 35.0 41.0 35.0 41.0 24-25 37.8635 39.0 35.0 41.0 35.0 41.0 26-27 37.732749999999996 39.0 35.0 41.0 35.0 41.0 28-29 37.75575 39.0 35.0 41.0 35.0 41.0 30-31 37.64717179093005 39.5 35.0 41.0 33.5 41.0 32-33 37.5626275640026 39.5 35.0 41.0 33.0 41.0 34-35 37.60093127575591 40.0 35.0 41.0 33.0 41.0 36-37 37.66195582514777 40.0 35.0 41.0 33.0 41.0 38-39 37.608710570951075 39.5 35.0 41.0 33.5 41.0 40-41 37.53922711658962 39.0 35.0 41.0 33.5 41.0 42-43 37.51905706355572 39.0 35.0 41.0 33.0 41.0 44-45 37.478215377742735 39.0 35.0 41.0 33.0 41.0 46-47 37.23910328764519 39.0 35.0 41.0 33.0 41.0 48-49 37.294863339597185 39.0 35.0 41.0 33.0 41.0 50-51 37.263764849409796 39.0 35.0 41.0 33.0 41.0 52-53 37.18298997997695 38.5 35.0 41.0 33.0 41.0 54-55 36.88821505359429 38.0 35.0 40.0 33.0 41.0 56-57 37.00046898402316 38.0 35.0 40.0 33.0 41.0 58-59 36.87081653675454 38.0 35.0 40.0 33.0 41.0 60-61 36.4772056330596 37.0 35.0 40.0 32.5 41.0 62-63 36.38937712243526 36.5 35.0 40.0 33.0 41.0 64-65 36.13658449883461 36.0 35.0 39.5 32.0 41.0 66-67 35.82212821190344 35.0 35.0 39.0 31.5 41.0 68-69 35.61456541805097 35.0 35.0 39.0 31.5 40.5 70-71 35.288863814478916 35.0 34.0 38.0 31.0 40.0 72-73 35.10643021772013 35.0 34.0 37.0 31.0 39.5 74-75 34.75197686615645 35.0 34.0 37.0 31.0 39.0 76-77 33.82617157393974 35.0 32.5 35.5 29.5 38.5 78-79 34.42994382463729 35.0 34.0 36.0 31.0 38.5 80-81 34.37942854169068 35.0 34.0 36.0 31.0 37.0 82-83 34.085337454837905 35.0 34.0 35.5 31.0 37.0 84-85 33.86785394265233 35.0 34.0 35.0 30.5 36.0 86-87 33.72824818673621 35.0 33.5 35.0 30.0 36.0 88-89 33.70126833886614 35.0 33.5 35.0 30.5 36.0 90-91 33.69158135873967 35.0 34.0 35.0 31.0 36.0 92-93 33.5377179115017 35.0 33.5 35.0 30.0 35.0 94-95 33.574946159957335 35.0 33.5 35.0 30.0 35.0 96-97 33.6443279922301 35.0 33.0 35.0 31.0 35.0 98-99 33.64933227219996 35.0 33.5 35.0 31.0 35.0 100-101 33.26087085244471 34.5 32.5 35.0 30.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 4.0 23 7.0 24 8.0 25 7.0 26 14.0 27 24.0 28 18.0 29 39.0 30 47.0 31 54.0 32 65.0 33 136.0 34 221.0 35 611.0 36 605.0 37 1140.0 38 884.0 39 113.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.325000000000001 50.075 19.900000000000002 16.7 2 16.05 25.85 22.05 36.05 3 15.299999999999999 26.650000000000002 43.925 14.124999999999998 4 14.625 25.1 43.85 16.425 5 16.163901458019104 25.26395173453997 44.06737053795877 14.504776269482154 6 17.075000000000003 24.925 42.075 15.925 7 16.925 25.474999999999998 43.0 14.6 8 14.975 24.875 44.05 16.1 9 14.924999999999999 24.75 44.85 15.475 10-11 15.387500000000001 25.224999999999998 43.625 15.7625 12-13 15.45 25.5 43.9 15.15 14-15 15.225 25.85 43.65 15.275 16-17 16.125 24.85 43.65 15.375 18-19 15.4 25.1 43.6625 15.837499999999999 20-21 15.6 25.900000000000002 43.0875 15.412500000000001 22-23 14.9875 25.4375 43.6625 15.9125 24-25 15.437500000000002 26.400000000000002 43.075 15.0875 26-27 15.525 25.8 43.1 15.575 28-29 15.375 26.85 42.475 15.299999999999999 30-31 14.931038846007846 26.85056307731241 42.36365936985955 15.854738706820196 32-33 16.337426588360916 27.78964228510411 39.54885210891618 16.324079017618793 34-35 17.27108517437821 28.99819369181603 36.015006252605254 17.7157148812005 36-37 17.88135593220339 29.929378531073446 34.85875706214689 17.33050847457627 38-39 17.455579246624023 30.788912579957355 34.92537313432836 16.830135039090262 40-41 17.21920548728208 30.58016576164618 34.995713060874536 17.2049156901972 42-43 17.355134825014343 30.49340218014917 34.36603557085485 17.78542742398164 44-45 17.24931664508704 30.858869227449286 33.90879010214358 17.9830240253201 46-47 17.439006785044032 30.835859679514943 33.89634762523459 17.828785910206438 48-49 18.047722342733188 31.496746203904557 33.304410701373826 17.15112075198843 50-51 17.00986078886311 31.235498839907194 33.67169373549884 18.08294663573086 52-53 16.897506925207757 31.870535063420323 33.138941536667154 18.09301647470477 54-55 17.36842105263158 33.81578947368421 31.65204678362573 17.163742690058477 56-57 16.913815886066658 32.09514021435913 32.917339597709585 18.07370430186463 58-59 16.956906729634003 34.29752066115703 32.06906729634002 16.67650531286895 60-61 16.859430604982208 32.74021352313167 32.05812574139976 18.34223013048636 62-63 17.580452920143028 33.26877234803337 31.570321811680575 17.580452920143028 64-65 17.238280664969295 33.48809345514452 31.780739853227498 17.49288602665868 66-67 17.7001806140879 33.80493678506923 30.70439494280554 17.79048765803733 68-69 17.231980617807388 34.32768019382193 30.920654149000605 17.519685039370078 70-71 17.53127860848337 34.46750076289289 30.851388465059504 17.149832163564234 72-73 16.930891180544865 34.27735878097584 31.429890718793292 17.36185931968601 74-75 17.662579543690825 34.61120595995654 30.001552072016143 17.72466242433649 76-77 17.191169563175198 35.321747299201505 30.31157037732895 17.17551276029435 78-79 17.757164154381318 35.911876759980125 27.944343216829555 18.386615868809013 80-81 17.920704845814978 38.87224669603524 24.158590308370044 19.048458149779734 82-83 18.384054102153407 38.52998754226731 22.584089695675388 20.501868659903895 84-85 19.662719770362397 37.244348762109794 23.484033010405454 19.608898457122354 86-87 18.5647143890094 37.92480115690528 24.54808387563268 18.96240057845264 88-89 18.168498168498168 38.71794871794872 23.827838827838825 19.28571428571429 90-91 18.254410399257196 39.07149489322192 24.215413184772515 18.458681522748375 92-93 18.54091854091854 38.95293895293895 22.887922887922887 19.61821961821962 94-95 18.636539204325995 38.837388953263805 23.174971031286212 19.351100811123985 96-97 17.526585269791255 38.440330838912956 24.025206774320598 20.007877116975187 98-99 19.687562587622672 38.79431203685159 22.57160024033647 18.946525135189265 100-101 17.966603255125765 40.75248361868527 21.87698161065314 19.403931515535827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 258.0 1 412.0 2 584.0 3 560.5 4 450.0 5 195.5 6 9.0 7 7.5 8 6.5 9 7.0 10 8.5 11 8.5 12 7.0 13 6.0 14 7.0 15 9.5 16 10.5 17 13.0 18 13.0 19 11.5 20 13.0 21 11.5 22 15.0 23 22.0 24 21.0 25 15.5 26 19.5 27 25.0 28 26.0 29 28.0 30 30.0 31 39.0 32 43.0 33 43.5 34 51.0 35 69.5 36 74.5 37 93.5 38 116.5 39 124.0 40 131.0 41 118.0 42 116.5 43 121.0 44 114.5 45 111.0 46 114.0 47 110.0 48 93.5 49 81.0 50 73.5 51 73.0 52 69.5 53 55.0 54 51.0 55 57.0 56 48.0 57 31.5 58 24.0 59 20.0 60 23.5 61 27.0 62 25.5 63 24.0 64 24.5 65 18.5 66 13.5 67 14.0 68 15.5 69 19.0 70 17.0 71 10.5 72 7.5 73 6.0 74 5.0 75 7.0 76 6.0 77 2.0 78 1.0 79 0.5 80 0.5 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5499999999999999 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01389274798555154 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 206.0 32-33 169.0 34-35 76.0 36-37 28.0 38-39 18.0 40-41 14.0 42-43 11.0 44-45 11.0 46-47 8.0 48-49 7.0 50-51 18.0 52-53 12.0 54-55 12.0 56-57 18.0 58-59 17.0 60-61 14.0 62-63 18.0 64-65 17.0 66-67 18.0 68-69 26.0 70-71 28.0 72-73 28.0 74-75 22.0 76-77 41.0 78-79 315.0 80-81 31.0 82-83 27.0 84-85 17.0 86-87 34.0 88-89 35.0 90-91 50.0 92-93 51.0 94-95 54.0 96-97 39.0 98-99 70.0 100-101 2440.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.675 #Duplication Level Percentage of deduplicated Percentage of total 1 98.0392156862745 71.25 2 0.41279669762641896 0.6 3 0.20639834881320948 0.44999999999999996 4 0.30959752321981426 0.8999999999999999 5 0.3439972480220158 1.25 6 0.20639834881320948 0.8999999999999999 7 0.03439972480220158 0.17500000000000002 8 0.03439972480220158 0.2 9 0.06879944960440317 0.44999999999999996 >10 0.13759889920880633 2.5250000000000004 >50 0.10319917440660474 6.625 >100 0.10319917440660474 14.674999999999999 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 273 6.825 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 205 5.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 2.725 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 2.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 2.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 1.825 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 1.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.6 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.42500000000000004 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 90 0.0 79.45 1 CTTTTTT 100 0.0 71.505 2 >>END_MODULE SRR13172455 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172455_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.6215 34.0 33.0 40.0 31.0 41.0 2 35.875 34.0 34.0 41.0 31.0 41.0 3 35.61275 34.0 34.0 40.0 31.0 41.0 4 37.75925 37.0 37.0 41.0 35.0 41.0 5 37.758 37.0 37.0 41.0 35.0 41.0 6 37.65525 37.0 37.0 41.0 35.0 41.0 7 37.76475 37.0 37.0 41.0 35.0 41.0 8 37.49625 37.0 37.0 41.0 35.0 41.0 9 38.33975 39.0 38.0 41.0 35.0 41.0 10-11 38.432249999999996 39.0 38.5 41.0 35.0 41.0 12-13 38.467625 39.0 38.5 41.0 35.0 41.0 14-15 38.259375000000006 39.0 37.0 41.0 35.5 41.0 16-17 37.791124999999994 39.5 35.0 41.0 35.0 41.0 18-19 37.55625 39.0 35.0 41.0 35.0 41.0 20-21 37.357 38.5 35.0 41.0 34.0 41.0 22-23 37.175 38.5 35.0 40.5 33.5 41.0 24-25 37.139375 38.0 35.0 40.5 34.0 41.0 26-27 37.10125 38.0 35.0 41.0 33.0 41.0 28-29 36.806375 38.0 35.0 40.0 33.0 41.0 30-31 37.02309043927649 38.0 35.0 40.5 33.0 41.0 32-33 36.96268626929758 38.5 35.0 41.0 33.0 41.0 34-35 37.03483673899034 39.0 35.0 41.0 33.0 41.0 36-37 37.026701640449474 38.0 35.0 41.0 33.0 41.0 38-39 36.908401771469585 38.0 35.0 40.0 33.0 41.0 40-41 36.91385614222848 38.0 35.0 40.0 33.0 41.0 42-43 36.769994157669885 37.5 35.0 40.0 33.0 41.0 44-45 36.59351770277449 37.0 35.0 40.0 33.0 41.0 46-47 36.45619757993345 37.0 35.0 40.0 33.0 41.0 48-49 36.33001969564834 36.0 35.0 40.0 33.0 41.0 50-51 36.01233069047043 35.5 35.0 39.5 32.5 40.5 52-53 36.05094919174002 35.5 35.0 39.5 32.5 40.5 54-55 36.269124301169725 35.5 35.0 40.0 33.0 41.0 56-57 36.14006156274547 35.0 35.0 40.0 33.0 41.0 58-59 36.20725167802637 35.0 35.0 40.0 33.0 41.0 60-61 36.041661275247066 35.0 35.0 40.0 32.5 41.0 62-63 35.62704157204372 35.0 35.0 39.0 31.0 41.0 64-65 35.58637420011167 35.0 35.0 39.0 31.5 41.0 66-67 35.41439404314875 35.0 34.0 38.5 31.0 41.0 68-69 35.32795305800045 35.0 34.0 38.0 31.5 41.0 70-71 35.092810709290575 35.0 34.0 37.0 31.0 40.0 72-73 34.95211712960823 35.0 34.0 37.0 31.0 39.5 74-75 34.67893058747961 35.0 34.0 36.0 31.0 39.0 76-77 34.59970107192338 35.0 34.0 36.0 31.0 39.0 78-79 34.42593974857468 35.0 34.0 35.5 30.5 38.0 80-81 34.56296860245602 35.0 34.0 36.0 31.0 37.5 82-83 34.31479084295986 35.0 34.0 35.5 31.0 37.0 84-85 34.03369159720078 35.0 34.0 35.0 31.0 36.5 86-87 33.82978693435249 35.0 34.0 35.0 31.0 36.0 88-89 33.66829685358873 35.0 33.5 35.0 30.5 36.0 90-91 33.532827274829984 35.0 33.0 35.0 30.5 36.0 92-93 33.43334171078431 35.0 33.0 35.0 30.0 35.0 94-95 33.518833788010305 35.0 33.0 35.0 30.5 35.0 96-97 33.57361383415795 35.0 33.0 35.0 30.5 35.0 98-99 33.615409632097965 35.0 33.0 35.0 30.5 35.0 100-101 33.23982446684231 34.5 32.5 35.0 30.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 5.0 21 6.0 22 7.0 23 6.0 24 11.0 25 20.0 26 19.0 27 22.0 28 42.0 29 27.0 30 39.0 31 57.0 32 85.0 33 115.0 34 243.0 35 655.0 36 741.0 37 1139.0 38 699.0 39 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.6 64.60000000000001 13.475000000000001 12.325 2 11.3 20.9 15.049999999999999 52.75 3 11.4 37.425000000000004 40.949999999999996 10.225 4 11.25 19.55 58.175 11.025 5 29.65 19.975 39.6 10.775 6 29.475 18.4 40.8 11.325000000000001 7 30.375000000000004 18.875 40.175 10.575 8 19.125 22.7 45.300000000000004 12.875 9 16.05 24.4 46.075 13.475000000000001 10-11 16.266566641660415 24.193548387096776 44.79869967491873 14.74118529632408 12-13 16.125 23.8875 45.1625 14.825 14-15 16.275000000000002 23.0125 45.75 14.9625 16-17 15.024999999999999 24.4125 45.387499999999996 15.174999999999999 18-19 16.075 24.2 44.75 14.975 20-21 16.025 23.8125 45.5625 14.6 22-23 15.275 24.1625 45.787499999999994 14.774999999999999 24-25 15.675 23.799999999999997 45.9625 14.5625 26-27 15.187500000000002 24.0125 45.5875 15.2125 28-29 15.737499999999999 23.0625 46.175 15.024999999999999 30-31 15.921219822109276 24.269377382465056 45.057179161372304 14.752223634053369 32-33 17.273221075502445 25.488864747419882 41.214014122759366 16.023900054318304 34-35 17.986120946041638 27.573998017278008 38.12491148562527 16.314969551055093 36-37 17.764010949430915 28.554963261777843 37.328915141910386 16.352110646880853 38-39 18.250618541696987 27.768883714160967 36.879639062727406 17.10085868141464 40-41 17.032806092560048 29.349736379613354 37.59519625073228 16.02226127709432 42-43 18.207282913165265 28.73359870263895 36.66519239274657 16.393925991449212 44-45 17.818558531892855 29.64333284001776 35.770312268758325 16.767796359331065 46-47 18.412839946500224 29.855847822856298 34.804577203150544 16.926735027492942 48-49 17.519773168183853 29.69706014027757 36.203551708700196 16.579614982838383 50-51 18.659067046647667 29.053547322633865 34.858257087145645 17.429128543572823 52-53 17.372306765104717 30.827180955250867 35.45276480337502 16.347747476269397 54-55 18.611700515307668 30.160654743861777 34.358896635344045 16.86874810548651 56-57 17.50990551661079 31.149039926851568 34.79122218835721 16.549832368180432 58-59 18.0510937739024 31.543521493039623 33.66987914945694 16.73550558360104 60-61 17.81307834669957 31.770512029611353 33.65206662553979 16.76434299814929 62-63 17.24352009933261 30.62238087847276 34.61120595995654 17.522893062238087 64-65 17.902294365537692 32.21476510067114 33.4633994068987 16.41954112689246 66-67 18.25035338463955 32.38573896654625 33.68933563687765 15.674572011936547 68-69 18.574821852731592 32.16152019002375 32.177355502771185 17.086302454473476 70-71 18.725099601593627 32.286852589641434 33.179282868525895 15.808764940239046 72-73 18.32553637683497 32.60203258590095 32.61816422003549 16.454266817228586 74-75 18.353708231458842 32.86063569682152 32.355338223308884 16.43031784841076 76-77 18.687118588157677 33.10242454230579 32.673593930397494 15.53686293913904 78-79 19.241516966067866 35.728542914171655 28.323353293413174 16.706586826347305 80-81 18.34158415841584 40.17326732673268 24.183168316831683 17.301980198019802 82-83 17.518796992481203 40.802005012531325 24.711779448621556 16.967418546365913 84-85 18.91960436216079 40.14709611970581 22.97742835404514 17.955871164088258 86-87 18.360572012257407 40.85801838610828 22.829417773238 17.95199182839632 88-89 18.658064516129034 40.92903225806452 23.53548387096774 16.87741935483871 90-91 18.9281045751634 39.81699346405229 22.797385620915033 18.457516339869283 92-93 18.355437665782492 40.61007957559682 23.28912466843501 17.745358090185675 94-95 17.760514331636752 41.86980980444683 22.42164478971337 17.948031074203055 96-97 18.671747414262384 41.31736526946108 22.264561785519867 17.746325530756668 98-99 17.674805771365147 40.64927857935627 23.446170921198668 18.22974472807991 100-101 17.56440281030445 41.80327868852459 22.540983606557376 18.091334894613585 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 213.0 1 423.0 2 651.0 3 628.5 4 516.0 5 224.5 6 6.0 7 9.5 8 8.0 9 5.0 10 4.5 11 4.5 12 8.5 13 10.5 14 8.5 15 7.5 16 13.5 17 18.0 18 15.0 19 13.0 20 14.0 21 13.5 22 16.0 23 18.0 24 15.5 25 15.0 26 15.0 27 17.5 28 17.5 29 18.5 30 23.5 31 27.5 32 36.5 33 48.0 34 57.0 35 66.0 36 76.5 37 86.5 38 93.0 39 106.5 40 126.5 41 139.0 42 138.5 43 132.0 44 128.5 45 134.0 46 129.5 47 125.5 48 119.5 49 97.0 50 84.5 51 76.5 52 74.5 53 64.5 54 55.0 55 62.0 56 56.5 57 40.5 58 36.5 59 37.5 60 30.0 61 24.0 62 21.5 63 19.5 64 20.5 65 21.5 66 21.5 67 20.5 68 21.0 69 17.5 70 13.0 71 13.0 72 9.0 73 6.5 74 5.5 75 5.5 76 6.0 77 3.0 78 3.0 79 2.5 80 1.0 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.025 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 263.0 32-33 185.0 34-35 71.0 36-37 40.0 38-39 24.0 40-41 22.0 42-43 14.0 44-45 14.0 46-47 12.0 48-49 16.0 50-51 16.0 52-53 18.0 54-55 21.0 56-57 13.0 58-59 23.0 60-61 21.0 62-63 17.0 64-65 19.0 66-67 26.0 68-69 21.0 70-71 36.0 72-73 32.0 74-75 32.0 76-77 88.0 78-79 929.0 80-81 25.0 82-83 27.0 84-85 13.0 86-87 18.0 88-89 25.0 90-91 28.0 92-93 19.0 94-95 26.0 96-97 32.0 98-99 55.0 100-101 1759.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.325 #Duplication Level Percentage of deduplicated Percentage of total 1 97.79594738713118 68.77499999999999 2 0.42659082829719164 0.6 3 0.4976892996800568 1.05 4 0.3199431212228937 0.8999999999999999 5 0.14219694276573053 0.5 6 0.10664770707429791 0.44999999999999996 7 0.14219694276573053 0.7000000000000001 8 0.03554923569143263 0.2 9 0.07109847138286526 0.44999999999999996 >10 0.2488446498400284 3.3000000000000003 >50 0.03554923569143263 1.825 >100 0.17774617845716317 21.25 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 318 7.95 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 170 4.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 3.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 3.175 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 2.675 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 1.825 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 1.0250000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27 0.675 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 20 4.2762083E-5 77.325 2 ACTTTTT 85 0.0 72.77647 1 ACATGGG 70 0.0 66.27857 1 CTTTTTT 95 0.0 65.11579 2 CATGGGA 35 9.621215E-6 55.232143 2 >>END_MODULE Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256816 spots for SRR13172455.sra Written 1256816 spots for SRR13172455.sra Read 1256824 spots for SRR13172455.sra Written 1256824 spots for SRR13172455.sra SRR ids: ['SRR13172455.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_j5vsqe78 SRR13172455.sra spots: 25136328 blocks: [[1, 1256816], [1256817, 2513632], [2513633, 3770448], [3770449, 5027264], [5027265, 6284080], [6284081, 7540896], [7540897, 8797712], [8797713, 10054528], [10054529, 11311344], [11311345, 12568160], [12568161, 13824976], [13824977, 15081792], [15081793, 16338608], [16338609, 17595424], [17595425, 18852240], [18852241, 20109056], [20109057, 21365872], [21365873, 22622688], [22622689, 23879504], [23879505, 25136328]] SRR13172455 file size 5324562 SRR13172455 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172455 SRR13172455_1.fastq SRR13172455_2.fastq Input file: SRR13172455_1.fastq Paired file: SRR13172455_2.fastq trimmed: SRR13172455-trimmed-pair1.fastq, SRR13172455-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:58:15 2024 >> started Fri Dec 6 10:58:45 2024 >> done (29.660s) 25136328 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 8 ( 0.00%) empty read pairs filtered out after trimming by size control 25136319 (100.00%) read pairs available; of these: 1473344 ( 5.86%) trimmed read pairs available after processing 23662975 (94.14%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 24 1 0.00% 25 0 0.00% 26 0 0.00% 27 4 0.00% 28 8 0.00% 29 467 0.00% 30 2042 0.01% 31 4129 0.02% 32 5297 0.02% 33 5776 0.02% 34 6328 0.03% 35 6565 0.03% 36 7169 0.03% 37 7977 0.03% 38 8817 0.04% 39 9912 0.04% 40 10977 0.04% 41 11961 0.05% 42 13536 0.05% 43 15443 0.06% 44 18185 0.07% 45 20832 0.08% 46 23769 0.09% 47 27924 0.11% 48 32585 0.13% 49 37522 0.15% 50 42143 0.17% 51 49527 0.20% 52 55541 0.22% 53 62148 0.25% 54 86975 0.35% 55 92305 0.37% 56 95989 0.38% 57 100374 0.40% 58 110036 0.44% 59 118811 0.47% 60 130950 0.52% 61 141310 0.56% 62 163840 0.65% 63 213089 0.85% 64 270839 1.08% 65 1172423 4.66% 66 1939515 7.72% 67 1023264 4.07% 68 434234 1.73% 69 268282 1.07% 70 230123 0.92% 71 217810 0.87% 72 209198 0.83% 73 202252 0.80% 74 210589 0.84% 75 200979 0.80% 76 201773 0.80% 77 225302 0.90% 78 340915 1.36% 79 290970 1.16% 80 271919 1.08% 81 255562 1.02% 82 295207 1.17% 83 312798 1.24% 84 331762 1.32% 85 334617 1.33% 86 399201 1.59% 87 440519 1.75% 88 614442 2.44% 89 6021127 23.95% 90 190325 0.76% 91 57643 0.23% 92 49756 0.20% 93 51305 0.20% 94 58724 0.23% 95 69989 0.28% 96 85805 0.34% 97 127919 0.51% 98 314268 1.25% 99 399346 1.59% 100 1183686 4.71% 101 4091667 16.28% 25136319 reads passed initial QC criterion=sequence-density sequence-density=0.11 sequence-density-rank=1 fanout-score=6.91 fanout-score-rank=24 prefix-density=0.16 prefix-fanout=4.9 sequence=AGCTAGCTAGCT criterion=fanout-score sequence-density=0.04 sequence-density-rank=5 fanout-score=227.48 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=32.7 sequence=CCCATGTACTCTGCGTTGATACCAC criterion=sequence-density sequence-density=0.13 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=44 prefix-density=0.03 prefix-fanout=1.0 sequence=ACATGGGGAAGA criterion=fanout-score sequence-density=0.02 sequence-density-rank=44 fanout-score=122.26 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=12.7 sequence=CCGCCGCCGCCCTACCACCACTAAGATCCATCACTCTCTTTAGTTGGGAGCACAGAATGTGATCGAAAAGACGTGAGCCGACTGTCTGAAGATGACGAGCCTTTGATTCTCCTCCCGTGCGTCGCCGCCAACCTAGGGCCAAGATCGAATAATGCAGACTGCTCCCTTCAGTTTATCTTACAGCTTATAATTTGTATTCGCCCAGTCTTAGCGTGGAAGTGCTGGCATTGTTAGCGTCGTCCCTGTGTTACTGTAAAACCATTTAGTAACTTTGGAAATGGAAGTAAAATCTGTTCACCCTTGT SRR13172455 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:59:34 Started mapping on | Dec 06 10:59:34 Finished on | Dec 06 11:08:34 Mapping speed, Million of reads per hour | 167.58 Number of input reads | 25136319 Average input read length | 166 UNIQUE READS: Uniquely mapped reads number | 13841055 Uniquely mapped reads % | 55.06% Average mapped length | 164.60 Number of splices: Total | 3752386 Number of splices: Annotated (sjdb) | 3314079 Number of splices: GT/AG | 3627379 Number of splices: GC/AG | 50299 Number of splices: AT/AC | 2721 Number of splices: Non-canonical | 71987 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.02% Deletion average length | 1.38 Insertion rate per base | 0.00% Insertion average length | 1.13 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1709475 % of reads mapped to multiple loci | 6.80% Number of reads mapped to too many loci | 115433 % of reads mapped to too many loci | 0.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 37.12% % of reads unmapped: other | 0.56% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 12806582 12806582 12806582 N_multimapping 1709475 1709475 1709475 N_noFeature 615572 8531966 5594332 N_ambiguous 389532 27063 36127 UnstrandedReadsAssigned:12835951 PositiveStrandReadsAssigned:5282026 NegativeStrandReadsAssigned:8210596 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=77 echo kmer=73 SRR13172455 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172455-trimmed-pair1.fastq SRR13172455-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,136,319 reads, 19,581,620 reads pseudoaligned [quant] estimated average fragment length: 143.691 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,178 rounds 52973 SRR13172455.ke.tsv 35125 SRR13172455.se.tsv 88098 total ==> SRR13172455.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 793.33 0 0 PNS24247 1044 901.309 0 0 PNS24249 1928 1785.31 0 0 PNS24246 1044 901.309 0 0 PNS24248 1044 901.309 0 0 PNS24244 1471 1328.31 479 29.2639 PNS24243 293 152.258 0 0 KQK14069 1603 1460.31 4 0.222285 KQK14071 474 332.156 0 0 ==> SRR13172455.se.tsv <== BRADI_1g14170v3 4 BRADI_1g53295v3 0 BRADI_1g59795v3 247 BRADI_1g07683v3 0 BRADI_1g00485v3 79 BRADI_1g20270v3 1622 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 189 BRADI_1g48960v3 0 SRR13172455 completed mapping pipeline successfully