Starting /dee2/code/volunteer_pipeline.sh SRR13172456 current disk space = 1551563997184 free memory = 1332039032 SRR13172456 SRAfilesize 2058255fd4a07e8fe536735d3b611dd8 SRR13172456.sra SRR13172456.sra file validated SRR13172456 is paired end SRR13172456 is conventional basespace SRR13172456 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172456_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.40675 34.0 33.0 34.0 31.0 40.0 2 34.7215 34.0 34.0 34.0 31.0 41.0 3 34.717 34.0 34.0 34.0 31.0 41.0 4 37.305 37.0 37.0 37.0 35.0 41.0 5 37.14275 37.0 37.0 37.0 35.0 41.0 6 37.1615 37.0 37.0 37.0 35.0 41.0 7 37.29725 37.0 37.0 37.0 35.0 41.0 8 37.30675 37.0 37.0 37.0 35.0 41.0 9 38.74425 39.0 39.0 39.0 37.0 41.0 10-11 38.729625 39.0 39.0 39.0 37.0 41.0 12-13 38.695125000000004 39.0 39.0 39.0 37.0 41.0 14-15 39.109875 40.0 37.5 41.0 36.5 41.0 16-17 38.655875 40.0 36.0 41.0 35.0 41.0 18-19 38.463375 40.0 35.5 41.0 35.0 41.0 20-21 38.2365 40.0 35.0 41.0 35.0 41.0 22-23 38.189 40.0 35.0 41.0 35.0 41.0 24-25 38.140375 40.0 35.0 41.0 35.0 41.0 26-27 38.016999999999996 40.0 35.0 41.0 35.0 41.0 28-29 37.98725 40.0 35.0 41.0 35.0 41.0 30-31 37.839674406736414 40.0 35.0 41.0 34.5 41.0 32-33 37.85187507343618 40.0 35.0 41.0 33.5 41.0 34-35 37.90284098705381 40.0 35.0 41.0 34.0 41.0 36-37 37.98210847137392 40.0 35.0 41.0 34.5 41.0 38-39 38.0179716932502 40.0 35.0 41.0 35.0 41.0 40-41 37.89603193063266 40.0 35.0 41.0 34.5 41.0 42-43 37.733945045242464 39.0 35.0 41.0 34.0 41.0 44-45 37.73811531143244 39.5 35.0 41.0 34.0 41.0 46-47 37.68660770826456 39.0 35.0 41.0 34.0 41.0 48-49 37.42532639396957 39.0 35.0 41.0 33.0 41.0 50-51 37.35545744607089 39.0 35.0 41.0 33.0 41.0 52-53 37.34974475638911 39.0 35.0 41.0 33.0 41.0 54-55 37.27107555580565 38.5 35.0 41.0 33.0 41.0 56-57 37.1823149374757 38.0 35.0 41.0 33.0 41.0 58-59 36.94689756728679 37.5 35.0 40.0 33.0 41.0 60-61 36.81327329066022 37.0 35.0 40.0 33.0 41.0 62-63 36.54465423072 37.0 35.0 40.0 33.0 41.0 64-65 36.358277959441295 36.0 35.0 39.5 33.0 41.0 66-67 36.04428842238782 35.5 35.0 39.0 33.0 41.0 68-69 35.62033947956082 35.0 35.0 39.0 31.5 40.5 70-71 35.38304279701855 35.0 35.0 38.0 31.0 40.0 72-73 35.02832823877064 35.0 34.0 37.0 31.0 39.5 74-75 34.78193522374994 35.0 34.0 37.0 31.0 39.0 76-77 34.05515283203558 35.0 33.0 35.5 30.0 38.5 78-79 34.59687243951984 35.0 34.0 36.0 31.0 38.5 80-81 34.57623458611127 35.0 34.0 36.0 31.5 37.0 82-83 34.38341756390537 35.0 34.0 35.5 31.5 37.0 84-85 34.28294649332284 35.0 34.0 35.0 31.5 36.5 86-87 34.03223664158874 35.0 34.0 35.0 31.5 36.0 88-89 33.71057917302891 35.0 34.0 35.0 31.0 36.0 90-91 33.914983603805744 35.0 34.0 35.0 31.0 36.0 92-93 33.65117172520161 35.0 34.0 35.0 30.5 35.0 94-95 33.655158393838434 35.0 34.0 35.0 31.0 35.0 96-97 33.75388065715799 35.0 34.0 35.0 31.0 35.0 98-99 33.67830923696057 35.0 34.0 35.0 31.0 35.0 100-101 33.28087820851793 34.5 32.5 35.0 29.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 3.0 23 3.0 24 3.0 25 9.0 26 7.0 27 16.0 28 23.0 29 22.0 30 34.0 31 51.0 32 77.0 33 117.0 34 212.0 35 574.0 36 672.0 37 1176.0 38 868.0 39 129.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.850000000000001 46.9 22.025 17.224999999999998 2 16.375 26.075 21.725 35.825 3 16.225 25.8 42.8 15.174999999999999 4 16.775000000000002 24.474999999999998 41.65 17.1 5 17.655935613682093 24.924547283702214 41.04627766599598 16.37323943661972 6 16.8 25.45 40.8 16.950000000000003 7 17.325 24.85 41.775 16.05 8 16.875 23.45 42.675000000000004 17.0 9 15.875 24.375 43.225 16.525000000000002 10-11 16.7625 25.687500000000004 41.6 15.950000000000001 12-13 16.5125 24.525 42.3125 16.650000000000002 14-15 16.525000000000002 25.174999999999997 41.7375 16.5625 16-17 17.05 24.712500000000002 41.349999999999994 16.8875 18-19 16.5 25.337500000000002 41.3125 16.85 20-21 16.8625 25.8 41.025 16.3125 22-23 16.25 26.0 42.125 15.625 24-25 17.3375 25.650000000000002 41.2875 15.725 26-27 16.775000000000002 25.837500000000002 40.925 16.4625 28-29 16.925 25.074999999999996 41.449999999999996 16.55 30-31 17.27490844803637 25.899734815001896 40.38388685440081 16.44146988256093 32-33 17.597987288135595 26.53601694915254 38.42690677966102 17.439088983050848 34-35 18.518009348364036 28.196315644762166 35.55127852625791 17.734396480615892 36-37 18.198273461431356 29.32330827067669 34.30799220272904 18.170426065162907 38-39 19.50364554122266 30.033651149747616 33.048233314638246 17.414469994391474 40-41 18.22153325817362 28.776775648252535 33.878241262683204 19.123449830890642 42-43 18.21140512239989 30.22498938729305 33.394651195698316 18.168954294608746 44-45 18.517466628798637 29.15364953138313 33.385401874467476 18.943481965350752 46-47 18.729525708588522 30.19512889901723 32.74462327303802 18.330722119356217 48-49 17.692417535341995 30.486934171069542 32.64315293445666 19.177495359131804 50-51 18.33285591521054 30.92237181323403 32.34030363792609 18.404468633629335 52-53 18.87579068430132 30.52041403105233 32.01552616446233 18.588269120184016 54-55 18.392960184650896 30.813618003462206 32.81881130986728 17.97461050201962 56-57 18.984228042251484 30.28505281435393 32.13717262335407 18.593546520040515 58-59 18.65194654270773 31.769320162696108 30.476467170249855 19.102266124346308 60-61 19.073370359543993 31.46740719087986 31.05817012569424 18.401052323881906 62-63 19.049714034315883 32.057486434961135 31.016278046634405 17.876521484088574 64-65 18.841857730746618 32.157554379776606 31.17283950617284 17.82774838330394 66-67 19.039881831610046 32.40768094534712 30.738552437223042 17.81388478581979 68-69 18.40463458110517 32.63517528223411 30.303030303030305 18.65715983363042 70-71 18.507462686567163 32.865671641791046 30.417910447761194 18.208955223880597 72-73 17.790121603362856 32.84792073262273 30.85122354000901 18.510734124005403 74-75 18.597975525003775 33.4642695271189 30.170720652666567 17.767034295210756 76-77 18.893448852409183 34.23012615899073 28.63657090743274 18.23985408116735 78-79 18.467677573822826 34.57302474062251 27.086991221069432 19.872306464485234 80-81 19.125041904123368 37.29466979550788 23.29869259135099 20.281595709017765 82-83 18.69079837618403 37.43234100135318 23.291610284167792 20.585250338294994 84-85 18.836376045043508 36.71728373997611 24.39856679747483 20.047773417505546 86-87 20.73422957600827 36.48741813167874 22.888659083074803 19.889693209238192 88-89 18.26086956521739 37.2695652173913 24.347826086956523 20.121739130434783 90-91 19.55032496047778 37.02792903565783 23.326892675215177 20.09485332864922 92-93 20.670590333511683 37.274835027644016 23.756019261637242 18.298555377207062 94-95 19.98917553671297 38.62529316254736 22.280353599134042 19.105177701605626 96-97 19.838798314709656 37.497710203333945 23.575746473713135 19.087745008243267 98-99 19.698660714285715 38.35565476190476 21.893601190476193 20.052083333333336 100-101 18.37573385518591 37.5146771037182 23.91389432485323 20.195694716242663 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 240.0 1 369.5 2 530.5 3 516.0 4 412.5 5 179.5 6 3.5 7 5.0 8 5.5 9 4.5 10 7.0 11 9.0 12 7.0 13 6.0 14 8.0 15 8.0 16 8.5 17 9.5 18 8.0 19 7.0 20 5.5 21 8.0 22 15.0 23 18.5 24 20.5 25 21.5 26 17.5 27 17.5 28 25.5 29 27.5 30 34.5 31 41.0 32 41.5 33 43.0 34 50.0 35 70.5 36 82.5 37 82.5 38 90.5 39 107.0 40 111.5 41 113.5 42 126.5 43 124.5 44 109.5 45 110.5 46 115.0 47 107.5 48 104.5 49 103.0 50 89.0 51 73.5 52 65.0 53 63.0 54 58.5 55 51.0 56 42.0 57 42.5 58 47.5 59 40.0 60 29.5 61 27.5 62 28.5 63 29.5 64 31.0 65 25.5 66 22.0 67 22.0 68 20.0 69 16.0 70 18.5 71 19.5 72 14.5 73 15.5 74 15.0 75 9.0 76 6.5 77 5.0 78 2.0 79 0.5 80 0.0 81 0.0 82 1.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.6 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.01419849495953429 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 173.0 32-33 172.0 34-35 54.0 36-37 30.0 38-39 19.0 40-41 16.0 42-43 12.0 44-45 11.0 46-47 8.0 48-49 10.0 50-51 16.0 52-53 10.0 54-55 12.0 56-57 11.0 58-59 22.0 60-61 13.0 62-63 6.0 64-65 16.0 66-67 18.0 68-69 15.0 70-71 22.0 72-73 16.0 74-75 22.0 76-77 33.0 78-79 272.0 80-81 31.0 82-83 23.0 84-85 30.0 86-87 24.0 88-89 28.0 90-91 42.0 92-93 35.0 94-95 32.0 96-97 47.0 98-99 57.0 100-101 2642.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.97500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 98.26608869623207 73.675 2 0.33344448149383127 0.5 3 0.3001000333444481 0.675 4 0.23341113704568192 0.7000000000000001 5 0.266755585195065 1.0 6 0.06668889629876625 0.3 7 0.03334444814938312 0.17500000000000002 8 0.03334444814938312 0.2 9 0.06668889629876625 0.44999999999999996 >10 0.20006668889629878 2.65 >50 0.10003334444814939 6.075 >100 0.10003334444814939 13.600000000000001 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 252 6.3 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191 4.775 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 2.5250000000000004 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 2.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 2.025 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 70 1.7500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.8999999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.44999999999999996 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.44999999999999996 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.35000000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTTT 80 0.0 83.4 2 ACTTTTT 85 0.0 78.49412 1 >>END_MODULE SRR13172456 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172456_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9065 34.0 33.0 40.0 31.0 41.0 2 36.192 34.0 34.0 41.0 31.0 41.0 3 35.9555 34.0 34.0 41.0 31.0 41.0 4 37.998 37.0 37.0 41.0 35.0 41.0 5 37.97075 37.0 37.0 41.0 35.0 41.0 6 37.998 37.0 37.0 41.0 35.0 41.0 7 37.91 37.0 37.0 41.0 35.0 41.0 8 37.63125 37.0 37.0 41.0 35.0 41.0 9 38.55025 39.0 38.0 41.0 35.0 41.0 10-11 38.658249999999995 39.0 39.0 41.0 35.5 41.0 12-13 38.682249999999996 39.0 39.0 41.0 36.5 41.0 14-15 38.39175 39.0 37.0 41.0 35.5 41.0 16-17 37.69575 39.0 35.0 41.0 35.0 41.0 18-19 37.725875 39.0 35.0 41.0 35.0 41.0 20-21 37.596125 39.0 35.0 41.0 35.0 41.0 22-23 37.4915 39.0 35.0 40.5 35.0 41.0 24-25 37.205124999999995 38.0 35.0 40.0 34.0 41.0 26-27 37.28275 38.0 35.0 41.0 34.0 41.0 28-29 36.95375 38.0 35.0 40.0 33.0 41.0 30-31 37.09830900621118 38.0 35.0 41.0 33.0 41.0 32-33 37.18602105387681 39.0 35.0 41.0 33.0 41.0 34-35 37.30331237973101 39.0 35.0 41.0 33.0 41.0 36-37 37.21268720950249 38.5 35.0 41.0 33.0 41.0 38-39 36.96398147268731 38.0 35.0 40.5 33.0 41.0 40-41 36.960621224543644 38.0 35.0 40.0 33.0 41.0 42-43 36.9600201364907 38.0 35.0 40.0 33.0 41.0 44-45 36.696884022060985 37.0 35.0 40.0 33.0 41.0 46-47 36.49772815981832 36.5 35.0 40.0 32.5 41.0 48-49 36.378801183543196 36.0 35.0 40.0 33.0 41.0 50-51 36.00662329208629 35.5 35.0 39.5 32.5 40.5 52-53 36.13864004804623 36.0 35.0 39.5 33.0 40.5 54-55 36.37516856468913 35.5 35.0 40.0 33.0 41.0 56-57 36.429905509361575 35.0 35.0 40.0 33.0 41.0 58-59 36.205913761908306 35.0 35.0 40.0 33.0 41.0 60-61 35.935616778631086 35.0 35.0 39.0 33.0 41.0 62-63 35.77575312956188 35.0 35.0 39.0 32.5 41.0 64-65 35.611076957894966 35.0 35.0 39.0 32.0 41.0 66-67 35.29164992148978 35.0 34.5 37.5 31.5 40.5 68-69 35.08450271337809 35.0 34.0 37.0 31.0 40.0 70-71 34.92802655866788 35.0 34.0 37.0 31.0 40.0 72-73 34.818763550267434 35.0 34.0 36.0 31.0 39.0 74-75 34.7011201991282 35.0 34.0 36.0 31.0 39.0 76-77 34.46341434995654 35.0 34.0 35.5 31.0 38.0 78-79 34.34442877727658 35.0 34.0 35.5 30.5 38.0 80-81 34.40680193579897 35.0 34.0 36.0 31.0 37.0 82-83 33.920403292069125 35.0 34.0 35.0 30.0 37.0 84-85 33.967397943029894 35.0 34.0 35.0 31.0 36.5 86-87 33.91015760081342 35.0 34.0 35.0 31.0 36.0 88-89 33.82511464383579 35.0 34.0 35.0 30.5 36.0 90-91 33.59313552202387 35.0 33.0 35.0 30.0 35.5 92-93 33.43153263991414 35.0 33.0 35.0 30.0 35.0 94-95 33.32437609494595 35.0 33.0 35.0 29.0 35.0 96-97 33.431074986626946 35.0 33.0 35.0 30.0 35.0 98-99 33.45050815322868 35.0 33.0 35.0 30.0 35.0 100-101 33.13396623964306 34.5 32.0 35.0 29.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 4.0 23 8.0 24 8.0 25 12.0 26 20.0 27 14.0 28 31.0 29 39.0 30 40.0 31 57.0 32 78.0 33 116.0 34 223.0 35 640.0 36 816.0 37 1162.0 38 662.0 39 68.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.4 65.17500000000001 13.475000000000001 11.95 2 11.55 18.875 15.4 54.175 3 11.375 38.35 39.875 10.4 4 10.75 17.75 59.12500000000001 12.375 5 31.125000000000004 17.875 39.900000000000006 11.1 6 31.5 18.075 39.4 11.025 7 31.175000000000004 18.224999999999998 39.675 10.925 8 20.974999999999998 21.925 43.275000000000006 13.825000000000001 9 16.425 24.575 44.574999999999996 14.424999999999999 10-11 16.25 22.7625 45.175 15.812499999999998 12-13 15.662499999999998 23.5125 44.824999999999996 16.0 14-15 16.537499999999998 23.5875 44.737500000000004 15.137500000000001 16-17 15.587500000000002 22.675 45.6 16.1375 18-19 16.1375 22.900000000000002 44.737500000000004 16.225 20-21 16.55 23.599999999999998 45.0125 14.8375 22-23 15.812499999999998 22.787499999999998 45.65 15.75 24-25 16.6 23.175 44.7 15.525 26-27 16.275000000000002 22.2625 45.3125 16.150000000000002 28-29 16.7125 22.9625 45.2 15.125 30-31 16.365717192268566 24.796541200406917 43.743641912512714 15.0940996948118 32-33 17.002569998647367 25.1859867442175 40.267820911673205 17.543622345461923 34-35 18.41098994476703 27.021668318934992 37.487607987537174 17.0797337487608 36-37 18.60934795152914 26.370455856895557 36.43969994229659 18.580496249278706 38-39 19.787419918462433 27.606290040768783 35.09027373325568 17.516016307513105 40-41 19.15703205034392 28.01112249378018 35.34318747255964 17.48865798331626 42-43 18.862363689949895 28.35249042145594 35.08694370763336 17.698202180960802 44-45 17.8486646884273 28.724035608308608 35.01483679525222 18.41246290801187 46-47 18.9873417721519 28.74162323157111 34.102755026061054 18.168279970215934 48-49 18.882899071578315 28.047319556753518 34.95058400718778 18.11919736448038 50-51 19.24176320144426 28.73476756431473 33.609146983601626 18.414322250639387 52-53 19.14186432995921 28.539054237800272 34.53693911466989 17.782142317570628 54-55 18.190086402910413 28.406851599211763 34.4095801121722 18.993481885705624 56-57 18.71291647649475 29.727673817130686 33.25726456716872 18.30214513920584 58-59 18.675435913123277 29.718568369531965 33.450596512695014 18.15539920464974 60-61 19.25026885850361 29.88170225841143 33.10800430173606 17.7600245813489 62-63 19.32344763670065 29.409947482236635 33.92029657089898 17.34630831016373 64-65 19.077926109903757 30.03725551071096 32.846941943495814 18.037876435889473 66-67 18.841710896034964 30.15922572588198 32.766156728067436 18.23290665001561 68-69 18.62621817038667 30.965105312794716 32.31688148381012 18.091795033008488 70-71 18.05094130675526 31.387438696408797 32.779623477297896 17.781996519538048 72-73 18.42356687898089 31.57643312101911 31.560509554140125 18.43949044585987 74-75 18.712887177018132 30.70133204943027 31.535869041887338 19.04991173166426 76-77 18.969706787623522 32.98234245909607 31.005993844160052 17.041956909120362 78-79 20.039370078740156 33.523622047244096 27.85433070866142 18.58267716535433 80-81 19.567916563198413 38.01837596225478 24.211571889744228 18.20213558480258 82-83 19.523212045169387 37.340025094102884 23.03638644918444 20.100376411543287 84-85 18.97422940879232 39.21172309247095 22.890348660939868 18.923698837796866 86-87 19.304038608077217 38.65887731775464 22.783845567691134 19.253238506477015 88-89 19.21106557377049 38.52459016393443 23.642418032786885 18.621926229508194 90-91 19.050090838307813 38.72307293018427 22.813392161951725 19.413444069556192 92-93 18.797785394147112 38.83469549169523 22.567888215133138 19.79963089902452 94-95 19.449639326743252 38.55196366550895 22.73577344376169 19.262623563986107 96-97 18.89100298994292 39.49442783365045 22.343027996738243 19.27154117966839 98-99 18.663721700717836 38.62506902263942 23.16399779127554 19.5472114853672 100-101 18.117854001759014 40.16417472881853 21.98768689533861 19.730284374083844 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 196.0 1 399.5 2 645.0 3 644.5 4 522.5 5 225.5 6 8.0 7 7.5 8 6.0 9 5.0 10 7.5 11 7.5 12 4.0 13 5.0 14 6.0 15 7.5 16 8.5 17 6.5 18 6.0 19 10.5 20 13.0 21 11.5 22 11.0 23 14.0 24 17.5 25 19.5 26 18.0 27 15.0 28 18.5 29 20.5 30 19.5 31 24.5 32 30.5 33 37.0 34 46.5 35 55.5 36 64.5 37 77.0 38 87.0 39 102.5 40 116.5 41 125.5 42 125.0 43 117.0 44 124.0 45 121.5 46 113.0 47 119.0 48 115.5 49 95.0 50 87.5 51 97.0 52 96.5 53 82.5 54 75.5 55 70.0 56 63.0 57 53.0 58 47.5 59 48.5 60 42.5 61 32.0 62 28.5 63 34.0 64 32.0 65 29.0 66 29.5 67 29.5 68 30.5 69 24.5 70 15.5 71 11.5 72 11.5 73 10.0 74 8.0 75 9.0 76 6.5 77 2.5 78 2.5 79 3.0 80 2.5 81 2.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 244.0 32-33 199.0 34-35 79.0 36-37 38.0 38-39 17.0 40-41 26.0 42-43 23.0 44-45 14.0 46-47 16.0 48-49 17.0 50-51 15.0 52-53 11.0 54-55 11.0 56-57 17.0 58-59 15.0 60-61 16.0 62-63 16.0 64-65 17.0 66-67 25.0 68-69 21.0 70-71 19.0 72-73 23.0 74-75 28.0 76-77 67.0 78-79 1006.0 80-81 22.0 82-83 18.0 84-85 8.0 86-87 15.0 88-89 26.0 90-91 28.0 92-93 24.0 94-95 30.0 96-97 28.0 98-99 59.0 100-101 1762.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.95 #Duplication Level Percentage of deduplicated Percentage of total 1 97.85059901338971 69.425 2 0.5285412262156448 0.75 3 0.21141649048625794 0.44999999999999996 4 0.49330514446793516 1.4000000000000001 5 0.21141649048625794 0.75 6 0.10570824524312897 0.44999999999999996 7 0.07047216349541931 0.35000000000000003 8 0.035236081747709654 0.2 9 0.10570824524312897 0.675 >10 0.14094432699083861 1.775 >50 0.07047216349541931 3.3000000000000003 >100 0.1761804087385483 20.474999999999998 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 297 7.425 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 162 4.05 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 3.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 2.9250000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 108 2.7 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 1.975 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 1.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.575 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.5499999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGGG 70 0.0 76.512505 1 CATGGGG 35 1.2914825E-9 76.512505 2 ATGGGGA 15 0.0014513446 76.5125 3 ACTTTTT 110 0.0 76.5125 1 CATGGGT 15 0.0014513446 76.5125 2 CTTTTTT 125 0.0 67.331 2 ATGGGTG 20 0.004541672 57.384373 3 >>END_MODULE Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498312 spots for SRR13172456.sra Written 1498312 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra Read 1498298 spots for SRR13172456.sra Written 1498298 spots for SRR13172456.sra SRR ids: ['SRR13172456.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_cpr6xnz1 SRR13172456.sra spots: 29965974 blocks: [[1, 1498298], [1498299, 2996596], [2996597, 4494894], [4494895, 5993192], [5993193, 7491490], [7491491, 8989788], [8989789, 10488086], [10488087, 11986384], [11986385, 13484682], [13484683, 14982980], [14982981, 16481278], [16481279, 17979576], [17979577, 19477874], [19477875, 20976172], [20976173, 22474470], [22474471, 23972768], [23972769, 25471066], [25471067, 26969364], [26969365, 28467662], [28467663, 29965974]] SRR13172456 file size 6426518 SRR13172456 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172456 SRR13172456_1.fastq SRR13172456_2.fastq Input file: SRR13172456_1.fastq Paired file: SRR13172456_2.fastq trimmed: SRR13172456-trimmed-pair1.fastq, SRR13172456-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:04:08 2024 >> started Fri Dec 6 11:04:38 2024 >> done (30.110s) 29965974 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 11 ( 0.00%) empty read pairs filtered out after trimming by size control 29965962 (100.00%) read pairs available; of these: 1454210 ( 4.85%) trimmed read pairs available after processing 28511752 (95.15%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 2 0.00% 27 0 0.00% 28 11 0.00% 29 374 0.00% 30 2105 0.01% 31 4492 0.01% 32 5694 0.02% 33 6492 0.02% 34 7081 0.02% 35 7350 0.02% 36 8080 0.03% 37 8810 0.03% 38 9626 0.03% 39 10839 0.04% 40 11804 0.04% 41 12529 0.04% 42 13941 0.05% 43 15669 0.05% 44 17985 0.06% 45 20592 0.07% 46 23205 0.08% 47 27324 0.09% 48 30922 0.10% 49 36345 0.12% 50 39252 0.13% 51 45779 0.15% 52 51647 0.17% 53 59349 0.20% 54 88397 0.29% 55 93396 0.31% 56 93308 0.31% 57 98709 0.33% 58 107259 0.36% 59 117830 0.39% 60 129272 0.43% 61 141815 0.47% 62 168769 0.56% 63 222607 0.74% 64 300457 1.00% 65 1312324 4.38% 66 2153512 7.19% 67 1178466 3.93% 68 497595 1.66% 69 304263 1.02% 70 256978 0.86% 71 244573 0.82% 72 234069 0.78% 73 229438 0.77% 74 236109 0.79% 75 228791 0.76% 76 229057 0.76% 77 281364 0.94% 78 405076 1.35% 79 325915 1.09% 80 302118 1.01% 81 287811 0.96% 82 328661 1.10% 83 353317 1.18% 84 374743 1.25% 85 386505 1.29% 86 481592 1.61% 87 527277 1.76% 88 861550 2.88% 89 7368466 24.59% 90 216351 0.72% 91 66868 0.22% 92 58805 0.20% 93 60911 0.20% 94 70227 0.23% 95 86167 0.29% 96 106213 0.35% 97 166802 0.56% 98 405449 1.35% 99 532202 1.78% 100 1659358 5.54% 101 5107920 17.05% 29965962 reads passed initial QC criterion=sequence-density sequence-density=0.14 sequence-density-rank=1 fanout-score=2.47 fanout-score-rank=30 prefix-density=0.13 prefix-fanout=2.5 sequence=TGCCGCACTTGCAG criterion=fanout-score sequence-density=0.04 sequence-density-rank=14 fanout-score=248.34 fanout-score-rank=1 prefix-density=0.30 prefix-fanout=31.4 sequence=CCCATGTACTCTGCGTTGATACC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=3.60 fanout-score-rank=5 prefix-density=0.21 prefix-fanout=3.1 sequence=AGCTAGCTAGCT criterion=fanout-score sequence-density=0.07 sequence-density-rank=25 fanout-score=58.00 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=12.0 sequence=GAAGAAGAAGAAACAACTCCGGCCATGGCGGGCATCATCCACAAGATCGAGG SRR13172456 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:06:26 Started mapping on | Dec 06 11:06:26 Finished on | Dec 06 11:16:39 Mapping speed, Million of reads per hour | 175.98 Number of input reads | 29965962 Average input read length | 168 UNIQUE READS: Uniquely mapped reads number | 17653342 Uniquely mapped reads % | 58.91% Average mapped length | 166.29 Number of splices: Total | 5145834 Number of splices: Annotated (sjdb) | 4613234 Number of splices: GT/AG | 4988452 Number of splices: GC/AG | 68270 Number of splices: AT/AC | 3914 Number of splices: Non-canonical | 85198 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.01% Deletion average length | 1.37 Insertion rate per base | 0.00% Insertion average length | 1.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1865704 % of reads mapped to multiple loci | 6.23% Number of reads mapped to too many loci | 105560 % of reads mapped to too many loci | 0.35% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 34.00% % of reads unmapped: other | 0.51% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 14199241 14199241 14199241 N_multimapping 1865704 1865704 1865704 N_noFeature 705053 11055305 6900440 N_ambiguous 475097 31923 46703 UnstrandedReadsAssigned:16473192 PositiveStrandReadsAssigned:6566114 NegativeStrandReadsAssigned:10706199 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=78 echo kmer=73 SRR13172456 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172456-trimmed-pair1.fastq SRR13172456-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,965,962 reads, 23,577,198 reads pseudoaligned [quant] estimated average fragment length: 149.946 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,138 rounds 52973 SRR13172456.ke.tsv 35125 SRR13172456.se.tsv 88098 total ==> SRR13172456.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 787.169 0 0 PNS24247 1044 895.054 0 0 PNS24249 1928 1779.05 0 0 PNS24246 1044 895.054 0 0 PNS24248 1044 895.054 0 0 PNS24244 1471 1322.05 709 35.8326 PNS24243 293 146.636 0 0 KQK14069 1603 1454.05 78 3.58423 KQK14071 474 325.951 0 0 ==> SRR13172456.se.tsv <== BRADI_1g14170v3 76 BRADI_1g53295v3 0 BRADI_1g59795v3 427 BRADI_1g07683v3 0 BRADI_1g00485v3 234 BRADI_1g20270v3 1761 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 574 BRADI_1g48960v3 0 SRR13172456 completed mapping pipeline successfully