Starting /dee2/code/volunteer_pipeline.sh SRR13172457 current disk space = 1551587287040 free memory = 1602201664 SRR13172457 SRAfilesize 9ddbdcbffa80a0dff90ab8e35255a581 SRR13172457.sra SRR13172457.sra file validated SRR13172457 is paired end SRR13172457 is conventional basespace SRR13172457 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172457_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.2765 34.0 33.0 34.0 31.0 40.0 2 34.728 34.0 34.0 34.0 31.0 41.0 3 34.68075 34.0 34.0 34.0 31.0 41.0 4 37.25025 37.0 37.0 37.0 35.0 41.0 5 37.1565 37.0 37.0 37.0 35.0 41.0 6 37.28325 37.0 37.0 37.0 35.0 41.0 7 37.2575 37.0 37.0 37.0 35.0 41.0 8 37.35 37.0 37.0 37.0 35.0 41.0 9 38.781 39.0 39.0 39.0 37.0 41.0 10-11 38.759 39.0 39.0 39.0 37.0 41.0 12-13 38.7275 39.0 39.0 39.0 37.0 41.0 14-15 39.1425 40.0 37.5 41.0 36.5 41.0 16-17 38.566125 40.0 35.0 41.0 35.0 41.0 18-19 38.371 40.0 35.0 41.0 35.0 41.0 20-21 38.23675 40.0 35.0 41.0 35.0 41.0 22-23 38.0905 40.0 35.0 41.0 35.0 41.0 24-25 37.954125 40.0 35.0 41.0 35.0 41.0 26-27 37.948499999999996 40.0 35.0 41.0 35.0 41.0 28-29 37.902 40.0 35.0 41.0 35.0 41.0 30-31 37.80933196721311 40.0 35.0 41.0 34.5 41.0 32-33 37.89529588544231 40.0 35.0 41.0 33.5 41.0 34-35 37.859685541844456 40.0 35.0 41.0 33.0 41.0 36-37 37.98933820034601 40.0 35.0 41.0 34.0 41.0 38-39 37.91822794968 40.0 35.0 41.0 34.5 41.0 40-41 37.8193547968997 40.0 35.0 41.0 34.0 41.0 42-43 37.736271689995554 39.5 35.0 41.0 33.5 41.0 44-45 37.73567485004771 39.0 35.0 41.0 34.0 41.0 46-47 37.69481677959101 39.0 35.0 41.0 34.0 41.0 48-49 37.52518391298873 39.0 35.0 41.0 33.0 41.0 50-51 37.5612001024487 39.0 35.0 41.0 33.5 41.0 52-53 37.38802534342402 39.0 35.0 41.0 33.0 41.0 54-55 37.364393766216324 39.0 35.0 41.0 33.0 41.0 56-57 37.14067319015247 38.0 35.0 40.5 33.0 41.0 58-59 37.077096028042504 38.0 35.0 40.5 33.0 41.0 60-61 36.886311530282114 37.5 35.0 40.0 33.0 41.0 62-63 36.59209235894063 37.0 35.0 40.0 32.5 41.0 64-65 36.42190776002046 36.0 35.0 40.0 33.0 41.0 66-67 36.26559584607346 36.0 35.0 39.0 33.0 41.0 68-69 36.00713338644431 35.0 35.0 39.0 33.0 41.0 70-71 35.64005710842743 35.0 35.0 39.0 32.0 40.5 72-73 35.4769955623839 35.0 35.0 37.5 32.0 40.0 74-75 34.93947773431111 35.0 34.0 37.0 31.0 39.0 76-77 33.79074897030941 34.5 32.5 35.5 29.5 38.5 78-79 34.58711585296274 35.0 34.0 36.0 31.0 39.0 80-81 34.62594757280861 35.0 34.0 36.0 31.5 37.0 82-83 34.428130145780486 35.0 34.0 35.5 31.5 37.0 84-85 34.23729624270689 35.0 34.0 35.0 31.0 37.0 86-87 34.18717065668555 35.0 34.0 35.0 31.5 36.0 88-89 34.076723844014595 35.0 34.0 35.0 31.0 36.0 90-91 33.81760840263023 35.0 34.0 35.0 31.0 36.0 92-93 33.57876542391239 35.0 33.5 35.0 30.5 35.0 94-95 33.47292712012426 35.0 33.0 35.0 29.5 35.0 96-97 33.593998976737474 35.0 33.5 35.0 30.5 35.0 98-99 33.21737576751933 35.0 33.0 35.0 29.5 35.0 100-101 33.31429451745136 34.5 32.5 35.0 29.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 5.0 25 6.0 26 12.0 27 26.0 28 20.0 29 35.0 30 43.0 31 46.0 32 67.0 33 106.0 34 202.0 35 559.0 36 631.0 37 1183.0 38 922.0 39 133.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.575000000000001 49.975 18.275 18.175 2 17.8 25.5 22.7 34.0 3 14.299999999999999 27.875 42.85 14.975 4 15.725 25.424999999999997 42.475 16.375 5 17.97837566004526 24.767412622579833 41.91601709831531 15.338194619059593 6 17.299999999999997 25.1 41.199999999999996 16.400000000000002 7 18.025 23.95 41.325 16.7 8 15.425 25.900000000000002 42.95 15.725 9 16.6 25.025 42.15 16.225 10-11 16.3625 26.2125 42.1375 15.287500000000001 12-13 16.2125 25.5375 42.125 16.125 14-15 16.05 25.924999999999997 42.0375 15.987499999999999 16-17 16.8375 26.5 40.887499999999996 15.775 18-19 17.1875 26.2125 41.5625 15.037500000000001 20-21 16.05 26.35 41.725 15.875 22-23 15.8144768096012 25.890736342042754 42.13026628328541 16.164520565070635 24-25 16.1375 26.387500000000003 41.725 15.75 26-27 16.3125 25.324999999999996 42.662499999999994 15.7 28-29 16.575 26.5 41.9 15.024999999999999 30-31 15.70091093117409 26.720647773279353 40.5996963562753 16.978744939271255 32-33 17.538010136036274 28.23419578554281 37.71672445985596 16.51106961856495 34-35 17.135727159295715 29.79342853181755 35.46374601414114 17.607098294745597 36-37 17.88629327329018 29.862088376020264 34.36532507739938 17.88629327329018 38-39 17.217415969366048 30.77577648560488 34.222096156573535 17.784711388455538 40-41 18.54389721627409 30.763740185581728 34.00428265524626 16.68807994289793 42-43 17.81254485431319 30.716233673029997 32.88359408640735 18.587627386249462 44-45 17.706382365653365 31.782163953320847 33.453392882869906 17.058060798155886 46-47 17.805442964678633 31.991893456861607 32.88940359004053 17.313259988419226 48-49 17.273914936855856 32.443025112498184 31.804325736681665 18.47873421396429 50-51 17.51675896240163 31.885747595453225 32.77470125327893 17.82279218886622 52-53 17.554125219426563 33.45523698069046 31.480397893504975 17.510239906378 54-55 17.943075117370892 33.509389671361504 30.883215962441312 17.664319248826292 56-57 18.27178729689808 33.85524372230429 30.797636632200888 17.07533234859675 58-59 17.704771815073585 33.70001486546752 31.276943659878103 17.318269659580793 60-61 17.26047904191617 33.41317365269461 31.047904191616766 18.278443113772454 62-63 18.926638604930847 34.636199639206254 30.006013229104028 16.43114852675887 64-65 18.736778482925356 33.75642187972197 29.495315805379267 18.011483831973408 66-67 17.662258701930384 34.79252165982672 30.080559355525153 17.46466028271774 68-69 17.65967223158217 35.22744677592281 29.039669168325933 18.073211824169093 70-71 18.209876543209877 35.44753086419753 29.01234567901235 17.330246913580247 72-73 17.858253030774012 35.03263910475599 29.204227541187443 17.904880323282562 74-75 17.797274009086635 35.18721604261319 29.186902710324297 17.828607237975874 76-77 17.48659728792179 34.831283506780196 29.959003468937244 17.72311573636077 78-79 18.768521567336187 36.79618044122489 25.353967731313798 19.081330260125124 80-81 19.134631960006896 38.57955524909498 23.530425788657126 18.75538700224099 82-83 18.51529902642559 38.856050069541034 23.974269819193324 18.654381084840054 84-85 18.904686677198526 38.75724065297525 23.714235562576793 18.62383710724943 86-87 19.79740536698063 38.17309401101831 22.783010485160833 19.246490136840237 88-89 19.557394746311623 37.765383231378195 23.74955019791292 18.927671824397265 90-91 19.139981701738336 38.38975297346752 23.12900274473925 19.341262580054895 92-93 19.606021185653226 37.79966548968593 23.7316483924921 18.86266493216874 94-95 19.16429249762583 37.54985754985755 23.836657169990502 19.449192782526115 96-97 19.47876447876448 37.45173745173745 23.32046332046332 19.74903474903475 98-99 18.663776113519905 38.92392589672842 23.117855735120223 19.294442254631456 100-101 19.50663349917081 38.41210613598673 21.786898839137645 20.29436152570481 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 249.0 1 374.5 2 552.0 3 558.0 4 444.0 5 192.0 6 7.0 7 9.0 8 10.0 9 7.0 10 9.0 11 10.0 12 8.5 13 10.0 14 12.0 15 11.5 16 13.0 17 12.5 18 11.5 19 13.5 20 12.0 21 15.0 22 15.5 23 18.0 24 24.0 25 24.5 26 26.0 27 26.5 28 27.5 29 29.5 30 34.0 31 37.0 32 41.0 33 51.0 34 53.0 35 60.5 36 72.0 37 76.5 38 88.0 39 105.5 40 118.5 41 134.5 42 137.0 43 129.0 44 125.5 45 120.0 46 117.0 47 109.0 48 97.5 49 87.5 50 83.0 51 76.0 52 73.5 53 64.0 54 51.5 55 51.0 56 48.5 57 40.0 58 33.0 59 32.0 60 29.5 61 25.5 62 23.0 63 19.5 64 17.0 65 14.5 66 12.0 67 15.5 68 18.5 69 15.0 70 12.5 71 10.0 72 10.0 73 9.0 74 4.0 75 4.0 76 3.0 77 3.5 78 4.0 79 2.0 80 0.5 81 0.5 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 2.5 99 7.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.575 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0125 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.014405070584845865 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 191.0 32-33 184.0 34-35 65.0 36-37 27.0 38-39 26.0 40-41 18.0 42-43 15.0 44-45 18.0 46-47 10.0 48-49 11.0 50-51 14.0 52-53 10.0 54-55 20.0 56-57 23.0 58-59 22.0 60-61 19.0 62-63 10.0 64-65 24.0 66-67 23.0 68-69 25.0 70-71 20.0 72-73 24.0 74-75 24.0 76-77 26.0 78-79 241.0 80-81 28.0 82-83 22.0 84-85 41.0 86-87 28.0 88-89 47.0 90-91 48.0 92-93 52.0 94-95 42.0 96-97 58.0 98-99 62.0 100-101 2482.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.175 #Duplication Level Percentage of deduplicated Percentage of total 1 97.84293899561847 72.575 2 0.4381530165149983 0.65 3 0.3707448601280755 0.8250000000000001 4 0.3033367037411527 0.8999999999999999 5 0.20222446916076847 0.75 6 0.16852039096730706 0.75 7 0.03370407819346141 0.17500000000000002 8 0.16852039096730706 1.0 9 0.06740815638692282 0.44999999999999996 >10 0.20222446916076847 2.85 >50 0.10111223458038424 6.25 >100 0.10111223458038424 12.825000000000001 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 213 5.325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 183 4.575 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 2.9250000000000003 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 2.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 2.35 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 1.525 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37 0.9249999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.65 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA 5 0.125 TruSeq Adapter, Index 2 (100% over 51bp) ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTTT 95 0.0 84.582275 2 ACTTTTT 100 0.0 80.353165 1 >>END_MODULE SRR13172457 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172457_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.71 34.0 33.0 40.0 31.0 41.0 2 36.00325 34.0 34.0 41.0 31.0 41.0 3 35.9015 34.0 34.0 41.0 31.0 41.0 4 37.9875 37.0 37.0 41.0 35.0 41.0 5 37.95 37.0 37.0 41.0 35.0 41.0 6 37.969 37.0 37.0 41.0 35.0 41.0 7 37.9565 37.0 37.0 41.0 35.0 41.0 8 37.76375 37.0 37.0 41.0 35.0 41.0 9 38.6445 39.0 39.0 41.0 36.0 41.0 10-11 38.727875 39.0 39.0 41.0 37.0 41.0 12-13 38.7655 39.0 39.0 41.0 37.0 41.0 14-15 38.543125 40.0 37.0 41.0 36.0 41.0 16-17 37.917249999999996 39.5 35.0 41.0 35.0 41.0 18-19 37.617999999999995 39.0 35.0 41.0 35.0 41.0 20-21 37.582125000000005 39.0 35.0 41.0 35.0 41.0 22-23 37.429249999999996 39.0 35.0 40.5 34.5 41.0 24-25 37.228 38.0 35.0 41.0 34.5 41.0 26-27 37.458375000000004 39.0 35.0 41.0 35.0 41.0 28-29 37.165875 38.0 35.0 41.0 33.0 41.0 30-31 37.17657217030114 38.5 35.0 41.0 33.0 41.0 32-33 37.20316203517573 39.0 35.0 41.0 33.0 41.0 34-35 37.26913823553685 39.0 35.0 41.0 33.0 41.0 36-37 37.20137577921592 39.0 35.0 41.0 33.0 41.0 38-39 37.23919382092777 39.0 35.0 41.0 33.0 41.0 40-41 37.125316997952794 38.0 35.0 41.0 33.0 41.0 42-43 36.97298371198126 38.0 35.0 40.0 33.0 41.0 44-45 36.937695605252514 38.0 35.0 40.0 33.0 41.0 46-47 36.58863059324912 37.0 35.0 40.0 33.0 41.0 48-49 36.65749100093427 37.0 35.0 40.0 33.0 41.0 50-51 36.310975323403255 36.5 35.0 39.5 32.5 40.5 52-53 36.463222853100575 36.5 35.0 39.5 33.0 40.5 54-55 36.59386056760687 36.0 35.0 40.0 33.0 41.0 56-57 36.446220730639354 36.0 35.0 40.0 33.0 41.0 58-59 36.43294037112877 36.0 35.0 40.0 33.0 41.0 60-61 36.26237159665055 35.0 35.0 40.0 33.0 41.0 62-63 36.12312253078674 35.0 35.0 39.5 33.0 41.0 64-65 35.91862516154765 35.0 35.0 39.0 32.5 41.0 66-67 35.78709743520384 35.0 35.0 39.0 32.0 41.0 68-69 35.443496148793706 35.0 35.0 38.5 31.5 41.0 70-71 35.288946353300005 35.0 35.0 37.5 32.0 40.0 72-73 35.06889386808246 35.0 34.0 37.0 31.0 39.5 74-75 34.92779186760886 35.0 34.0 36.5 32.0 39.0 76-77 34.821919977370925 35.0 34.0 36.0 31.5 39.0 78-79 34.613961990316405 35.0 34.0 36.0 31.5 39.0 80-81 34.598407627757894 35.0 34.0 36.0 31.0 37.5 82-83 34.426942583921736 35.0 34.0 36.0 31.0 37.0 84-85 34.092828821172674 35.0 34.0 35.0 31.0 37.0 86-87 33.915593096742825 35.0 34.0 35.0 31.0 36.0 88-89 33.69544969783149 35.0 33.5 35.0 30.5 36.0 90-91 33.72414732370156 35.0 33.5 35.0 31.0 36.0 92-93 33.627142760142846 35.0 34.0 35.0 30.5 35.5 94-95 33.54712972851971 35.0 33.0 35.0 30.0 35.0 96-97 33.61997483536334 35.0 33.5 35.0 31.0 35.0 98-99 33.73507146464961 35.0 34.0 35.0 31.0 35.0 100-101 33.26215876326033 34.5 32.5 35.0 29.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 4.0 22 6.0 23 6.0 24 6.0 25 14.0 26 12.0 27 19.0 28 21.0 29 27.0 30 48.0 31 65.0 32 75.0 33 114.0 34 204.0 35 546.0 36 721.0 37 1272.0 38 750.0 39 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.15 63.075 12.025 13.750000000000002 2 11.5 19.7 16.975 51.824999999999996 3 10.274999999999999 34.8 42.875 12.049999999999999 4 11.899999999999999 18.325 57.4 12.375 5 27.975 18.325 42.95 10.75 6 27.500000000000004 18.275 41.9 12.325 7 27.224999999999998 19.45 42.225 11.1 8 18.525 21.175 46.125 14.174999999999999 9 16.6 22.95 46.949999999999996 13.5 10-11 15.775 22.8875 46.25 15.0875 12-13 15.6375 23.1375 46.400000000000006 14.825 14-15 16.4375 22.7125 46.275 14.575 16-17 15.837499999999999 22.4625 46.2625 15.437500000000002 18-19 15.825 23.0 46.6 14.575 20-21 15.4 23.7875 46.525 14.2875 22-23 16.175 22.787499999999998 46.0625 14.975 24-25 15.837499999999999 23.1625 46.362500000000004 14.637500000000001 26-27 15.187500000000002 23.95 45.8625 15.0 28-29 15.462500000000002 23.150000000000002 46.175 15.2125 30-31 16.441670911869586 23.11512990320937 45.147733061640345 15.295466123280693 32-33 17.16867469879518 26.06790799561884 41.01861993428258 15.744797371303395 34-35 18.06952025280092 27.01809824762999 37.5035909221488 17.408790577420284 36-37 18.24806879463635 27.430403731234517 36.59816353301268 17.723363941116453 38-39 18.656277565421934 26.992061158482798 36.57747721258453 17.77418406351073 40-41 18.254437869822485 27.707100591715978 36.59763313609468 17.440828402366865 42-43 18.946271766631938 27.995237386515853 35.451704122637295 17.606786724214913 44-45 18.41079460269865 28.065967016491754 36.74662668665667 16.776611694152923 46-47 18.06558363417569 29.136582430806257 35.018050541516246 17.779783393501805 48-49 18.50560775992725 29.539254319490755 34.38920885116702 17.565929069414974 50-51 18.177655677655675 29.945054945054945 34.6001221001221 17.277167277167276 52-53 19.13324112494237 28.968802827723987 33.84047948363301 18.057476563700632 54-55 18.584891086049744 28.765641897111077 35.22323497605438 17.426232040784797 56-57 18.16912335143522 29.65089216446858 34.42979053529868 17.75019394879752 58-59 18.262632563942606 30.598877105427324 33.889582033686835 17.248908296943235 60-61 18.17467797675149 30.442978322337417 33.82029531888156 17.562048382029534 62-63 18.216258879242307 30.071033938437253 33.67008681925809 18.042620363062355 64-65 18.413957176843777 30.864393338620143 33.49722442505947 17.224425059476605 66-67 19.13196106590075 30.237753310994098 32.98228817616084 17.64799744694431 68-69 18.538152610441767 30.939759036144576 34.15261044176707 16.369477911646587 70-71 19.164101733354933 30.55240563745343 32.54495383120039 17.73853879799125 72-73 18.28291087489779 31.79067865903516 32.34668847097302 17.57972199509403 74-75 17.65287621559255 31.13565188725894 33.2289434646448 17.98252843250371 76-77 18.567860116569527 31.973355537052456 32.03996669442132 17.418817651956704 78-79 18.230298242148923 34.12996247284219 29.409441042859964 18.230298242148923 80-81 18.66381359961959 37.63670946267237 25.130765572990967 18.56871136471707 82-83 18.501675442795595 37.31450454763044 25.8736237434179 18.310196266156055 84-85 18.76507477086348 37.09599614085866 24.60202604920405 19.536903039073806 86-87 20.38527188490612 36.99097781029017 24.09168495488905 18.532065349914657 88-89 18.9328743545611 38.62798131300713 23.530858126383084 18.908286206048686 90-91 19.323551355384232 38.249191743347424 23.476747077841335 18.95050982342701 92-93 19.263741805345436 37.87191124558749 23.928391326273324 18.935955622793745 94-95 18.793147532600358 38.22551777039121 23.446688826387113 19.534645870621326 96-97 19.927253832164197 38.58144972720187 23.356716030137697 18.13458041049623 98-99 18.94764815306936 37.629550890247145 24.20940738772256 19.213393568960935 100-101 18.633193863319384 38.18688981868898 24.909344490934448 18.270571827057182 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 185.0 1 393.0 2 666.0 3 678.0 4 562.0 5 253.0 6 7.5 7 11.0 8 14.0 9 9.5 10 4.5 11 5.0 12 6.0 13 6.5 14 7.0 15 7.5 16 7.5 17 8.0 18 9.0 19 11.5 20 12.0 21 14.0 22 16.0 23 10.5 24 12.0 25 16.5 26 18.0 27 17.0 28 17.0 29 25.0 30 29.5 31 35.0 32 35.0 33 36.0 34 51.5 35 57.0 36 68.0 37 82.5 38 93.5 39 111.5 40 114.5 41 124.0 42 128.5 43 124.0 44 131.5 45 138.0 46 135.0 47 123.5 48 103.0 49 96.5 50 99.5 51 85.0 52 73.5 53 65.0 54 60.5 55 63.0 56 59.5 57 51.0 58 40.5 59 31.5 60 26.0 61 22.5 62 23.5 63 18.0 64 14.0 65 19.5 66 25.5 67 27.0 68 23.0 69 16.0 70 12.5 71 10.0 72 6.5 73 6.0 74 5.5 75 4.0 76 3.0 77 3.5 78 3.0 79 2.5 80 1.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.5 97 1.0 98 2.0 99 5.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 276.0 32-33 224.0 34-35 60.0 36-37 35.0 38-39 19.0 40-41 21.0 42-43 26.0 44-45 12.0 46-47 21.0 48-49 22.0 50-51 26.0 52-53 19.0 54-55 13.0 56-57 17.0 58-59 20.0 60-61 20.0 62-63 13.0 64-65 17.0 66-67 20.0 68-69 30.0 70-71 23.0 72-73 25.0 74-75 31.0 76-77 78.0 78-79 824.0 80-81 17.0 82-83 14.0 84-85 20.0 86-87 20.0 88-89 18.0 90-91 30.0 92-93 26.0 94-95 29.0 96-97 42.0 98-99 53.0 100-101 1839.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.19999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 97.57834757834758 68.5 2 0.5698005698005698 0.8 3 0.3205128205128205 0.675 4 0.24928774928774927 0.7000000000000001 5 0.3561253561253561 1.25 6 0.2136752136752137 0.8999999999999999 7 0.03561253561253561 0.17500000000000002 8 0.14245014245014245 0.8 9 0.10683760683760685 0.675 >10 0.2136752136752137 2.625 >50 0.03561253561253561 1.975 >100 0.17806267806267806 20.925 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 287 7.175 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 3.6999999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 3.4750000000000005 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 136 3.4000000000000004 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 3.175 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 1.975 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.8750000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.5 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit GTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA 9 0.22499999999999998 Illumina Single End PCR Primer 1 (100% over 45bp) ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit GTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA 5 0.125 Illumina Single End PCR Primer 1 (100% over 45bp) ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGGA 15 0.0013713762 77.6125 3 CATGGGT 15 0.0013713762 77.6125 2 ACATGGG 80 0.0 72.76172 1 CATGGGG 60 0.0 71.14479 2 ACTTTTT 95 0.0 69.442764 1 CTTTTTT 100 0.0 65.97062 2 ATGGGGT 30 3.7707578E-6 64.677086 3 >>END_MODULE Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra Read 1291598 spots for SRR13172457.sra Written 1291598 spots for SRR13172457.sra Read 1291581 spots for SRR13172457.sra Written 1291581 spots for SRR13172457.sra SRR ids: ['SRR13172457.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_yftwmbdv SRR13172457.sra spots: 25831637 blocks: [[1, 1291581], [1291582, 2583162], [2583163, 3874743], [3874744, 5166324], [5166325, 6457905], [6457906, 7749486], [7749487, 9041067], [9041068, 10332648], [10332649, 11624229], [11624230, 12915810], [12915811, 14207391], [14207392, 15498972], [15498973, 16790553], [16790554, 18082134], [18082135, 19373715], [19373716, 20665296], [20665297, 21956877], [21956878, 23248458], [23248459, 24540039], [24540040, 25831637]] SRR13172457 file size 5496372 SRR13172457 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172457 SRR13172457_1.fastq SRR13172457_2.fastq Input file: SRR13172457_1.fastq Paired file: SRR13172457_2.fastq trimmed: SRR13172457-trimmed-pair1.fastq, SRR13172457-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:06:51 2024 >> started Fri Dec 6 11:07:16 2024 >> done (25.272s) 25831637 read pairs processed; of these: 4 ( 0.00%) short read pairs filtered out after trimming by size control 42 ( 0.00%) empty read pairs filtered out after trimming by size control 25831591 (100.00%) read pairs available; of these: 1661982 ( 6.43%) trimmed read pairs available after processing 24169609 (93.57%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 1 0.00% 21 1 0.00% 22 0 0.00% 23 1 0.00% 24 2 0.00% 25 4 0.00% 26 0 0.00% 27 5 0.00% 28 15 0.00% 29 510 0.00% 30 2626 0.01% 31 5551 0.02% 32 6929 0.03% 33 8073 0.03% 34 8875 0.03% 35 9448 0.04% 36 10437 0.04% 37 11607 0.04% 38 12869 0.05% 39 14279 0.06% 40 15782 0.06% 41 17250 0.07% 42 19253 0.07% 43 21606 0.08% 44 23930 0.09% 45 27482 0.11% 46 30391 0.12% 47 35030 0.14% 48 39914 0.15% 49 45095 0.17% 50 51751 0.20% 51 59065 0.23% 52 65795 0.25% 53 79359 0.31% 54 97975 0.38% 55 95422 0.37% 56 98597 0.38% 57 103227 0.40% 58 112514 0.44% 59 121512 0.47% 60 131637 0.51% 61 143704 0.56% 62 163391 0.63% 63 208036 0.81% 64 267977 1.04% 65 1109168 4.29% 66 1787149 6.92% 67 949739 3.68% 68 419607 1.62% 69 279317 1.08% 70 244296 0.95% 71 232992 0.90% 72 225168 0.87% 73 219833 0.85% 74 228333 0.88% 75 218922 0.85% 76 214931 0.83% 77 241665 0.94% 78 368986 1.43% 79 308077 1.19% 80 281842 1.09% 81 263565 1.02% 82 302125 1.17% 83 320883 1.24% 84 334756 1.30% 85 342117 1.32% 86 396926 1.54% 87 421356 1.63% 88 645550 2.50% 89 6030402 23.35% 90 210417 0.81% 91 62450 0.24% 92 52152 0.20% 93 53474 0.21% 94 62418 0.24% 95 73946 0.29% 96 90408 0.35% 97 135290 0.52% 98 324979 1.26% 99 420222 1.63% 100 1318132 5.10% 101 4473069 17.32% 25831591 reads passed initial QC criterion=sequence-density sequence-density=0.04 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=33 prefix-density=0.04 prefix-fanout=1.9 sequence=GTCGAATTTGTCAATTGAATTAACAAGGGAACTCCTTTATTGAGTTTTTATCCATGCATTCGTATTAGATCATGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=16 fanout-score=179.04 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=18.6 sequence=CTTCTTCTTGTC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=50 prefix-density=0.00 prefix-fanout=1.0 sequence=GTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA criterion=fanout-score sequence-density=0.04 sequence-density-rank=37 fanout-score=158.06 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=22.0 sequence=AAGAAGAAGAAA SRR13172457 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:09:07 Started mapping on | Dec 06 11:09:08 Finished on | Dec 06 11:22:34 Mapping speed, Million of reads per hour | 115.38 Number of input reads | 25831591 Average input read length | 167 UNIQUE READS: Uniquely mapped reads number | 11762075 Uniquely mapped reads % | 45.53% Average mapped length | 164.49 Number of splices: Total | 3169310 Number of splices: Annotated (sjdb) | 2746348 Number of splices: GT/AG | 3055148 Number of splices: GC/AG | 42273 Number of splices: AT/AC | 2319 Number of splices: Non-canonical | 69570 Mismatch rate per base, % | 0.35% Deletion rate per base | 0.04% Deletion average length | 1.54 Insertion rate per base | 0.01% Insertion average length | 1.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1732731 % of reads mapped to multiple loci | 6.71% Number of reads mapped to too many loci | 140389 % of reads mapped to too many loci | 0.54% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 46.58% % of reads unmapped: other | 0.64% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 15093895 15093895 15093895 N_multimapping 1732731 1732731 1732731 N_noFeature 591170 7128123 4933726 N_ambiguous 346161 25900 32191 UnstrandedReadsAssigned:10824744 PositiveStrandReadsAssigned:4608052 NegativeStrandReadsAssigned:6796158 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=77 echo kmer=73 SRR13172457 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172457-trimmed-pair1.fastq SRR13172457-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,831,591 reads, 18,534,755 reads pseudoaligned [quant] estimated average fragment length: 146.343 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,207 rounds 52973 SRR13172457.ke.tsv 35125 SRR13172457.se.tsv 88098 total ==> SRR13172457.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 790.728 0 0 PNS24247 1044 898.657 0 0 PNS24249 1928 1782.66 113.123 5.64741 PNS24246 1044 898.657 0 0 PNS24248 1044 898.657 0 0 PNS24244 1471 1325.66 435.877 29.2617 PNS24243 293 149.856 0 0 KQK14069 1603 1457.66 0 0 KQK14071 474 329.566 0 0 ==> SRR13172457.se.tsv <== BRADI_1g14170v3 0 BRADI_1g53295v3 19 BRADI_1g59795v3 1290 BRADI_1g07683v3 0 BRADI_1g00485v3 113 BRADI_1g20270v3 610 BRADI_1g74790v3 65 BRADI_1g09890v3 0 BRADI_1g77505v3 340 BRADI_1g48960v3 0 SRR13172457 completed mapping pipeline successfully