Starting /dee2/code/volunteer_pipeline.sh SRR13172458 current disk space = 1551525531648 free memory = 1605395588 SRR13172458 SRAfilesize 82a38f587236e145003d6a049f552a7c SRR13172458.sra SRR13172458.sra file validated SRR13172458 is paired end SRR13172458 is conventional basespace SRR13172458 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172458_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.48825 34.0 33.0 34.0 31.0 40.0 2 34.8715 34.0 34.0 34.0 31.0 41.0 3 34.88875 34.0 34.0 34.0 31.0 41.0 4 37.39025 37.0 37.0 37.0 35.0 41.0 5 37.19725 37.0 37.0 37.0 35.0 41.0 6 37.2065 37.0 37.0 37.0 35.0 41.0 7 37.3575 37.0 37.0 37.0 35.0 41.0 8 37.332 37.0 37.0 37.0 35.0 41.0 9 38.76525 39.0 39.0 39.0 37.0 41.0 10-11 38.72025 39.0 39.0 39.0 37.0 41.0 12-13 38.68875 39.0 39.0 39.0 37.0 41.0 14-15 39.069500000000005 40.0 37.0 41.0 36.5 41.0 16-17 38.562375 40.0 35.0 41.0 35.0 41.0 18-19 38.242625000000004 40.0 35.0 41.0 35.0 41.0 20-21 37.990375 40.0 35.0 41.0 35.0 41.0 22-23 37.8945 39.5 35.0 41.0 35.0 41.0 24-25 37.870125 39.5 35.0 41.0 35.0 41.0 26-27 37.84025 40.0 35.0 41.0 35.0 41.0 28-29 37.788624999999996 39.5 35.0 41.0 35.0 41.0 30-31 37.661863578356304 39.0 35.0 41.0 33.5 41.0 32-33 37.63505086199553 40.0 35.0 41.0 33.0 41.0 34-35 37.65393656432988 40.0 35.0 41.0 33.0 41.0 36-37 37.77641080681069 40.0 35.0 41.0 34.0 41.0 38-39 37.82742307662172 40.0 35.0 41.0 34.5 41.0 40-41 37.75292191770422 39.5 35.0 41.0 34.0 41.0 42-43 37.54564729115829 39.0 35.0 41.0 34.0 41.0 44-45 37.56036045731518 39.0 35.0 41.0 33.5 41.0 46-47 37.44560677443867 39.0 35.0 41.0 33.5 41.0 48-49 37.27011956096129 39.0 35.0 41.0 33.0 41.0 50-51 37.272937661344415 38.5 35.0 41.0 33.0 41.0 52-53 37.20656883654701 38.0 35.0 41.0 33.0 41.0 54-55 37.19586590015395 38.0 35.0 41.0 33.0 41.0 56-57 37.158389317681284 38.0 35.0 41.0 33.0 41.0 58-59 36.87866982963446 37.5 35.0 40.0 33.0 41.0 60-61 36.82376529135101 37.0 35.0 40.0 33.0 41.0 62-63 36.58954527228529 37.0 35.0 40.0 33.0 41.0 64-65 36.365416303718845 36.0 35.0 39.5 33.0 41.0 66-67 36.09300977041958 35.5 35.0 39.0 33.0 41.0 68-69 35.606269724998 35.0 35.0 39.0 32.0 40.5 70-71 35.374734100073624 35.0 35.0 38.0 32.0 40.0 72-73 35.105380234279316 35.0 34.0 37.0 31.0 39.5 74-75 34.82253518977394 35.0 34.0 37.0 31.0 39.0 76-77 34.193981273524265 35.0 33.5 35.5 30.0 39.0 78-79 34.70359792803221 35.0 34.0 36.0 31.5 38.5 80-81 34.600608689061644 35.0 34.0 36.0 32.0 37.5 82-83 34.417865558377514 35.0 34.0 35.5 31.5 37.0 84-85 34.236443516444545 35.0 34.0 35.0 31.5 36.5 86-87 34.05206375766937 35.0 34.0 35.0 31.0 36.0 88-89 33.803529899526055 35.0 33.5 35.0 31.0 36.0 90-91 33.931501849772275 35.0 34.0 35.0 31.0 36.0 92-93 33.75636704213642 35.0 34.0 35.0 31.0 35.5 94-95 33.63688396311967 35.0 33.5 35.0 30.5 35.0 96-97 33.80021746756307 35.0 34.0 35.0 31.0 35.0 98-99 33.82628817046387 35.0 34.0 35.0 31.0 35.0 100-101 33.316945511257316 34.5 32.5 35.0 29.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 2.0 23 3.0 24 4.0 25 13.0 26 10.0 27 8.0 28 26.0 29 25.0 30 44.0 31 42.0 32 66.0 33 128.0 34 236.0 35 593.0 36 651.0 37 1116.0 38 891.0 39 139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.65 51.175000000000004 20.674999999999997 14.499999999999998 2 15.875 27.125 20.95 36.05 3 14.625 28.175 43.325 13.875000000000002 4 14.725 26.3 43.475 15.5 5 16.620568267538346 26.527533316570278 41.211968820719136 15.63992959517224 6 15.65 26.825 42.225 15.299999999999999 7 16.400000000000002 26.825 41.975 14.799999999999999 8 14.799999999999999 25.75 43.4 16.05 9 15.5 26.35 43.5 14.649999999999999 10-11 15.7 27.1125 42.3375 14.85 12-13 14.575 26.987499999999997 43.6 14.8375 14-15 15.012500000000001 26.737499999999997 42.85 15.4 16-17 15.0875 26.787499999999998 42.8875 15.2375 18-19 14.649999999999999 26.75 43.4 15.2 20-21 15.437500000000002 27.275 42.625 14.662500000000001 22-23 15.25 27.500000000000004 42.3625 14.887500000000001 24-25 15.8875 26.737499999999997 42.4 14.975 26-27 14.637500000000001 27.400000000000002 42.9 15.0625 28-29 14.924999999999999 27.125 42.725 15.225 30-31 15.180601161909573 27.885829754988634 41.90452134377368 15.029047739328114 32-33 15.32460663757768 29.221208515139494 39.69324342192252 15.760941425360306 34-35 17.002605237899353 30.728095433977785 36.67900726724256 15.590292060880294 36-37 16.1970853573907 30.645385149201942 36.18320610687023 16.974323386537126 38-39 17.424772568229532 30.48285514345696 35.18544436668999 16.906927921623513 40-41 17.758717660292465 31.48200224971879 34.392575928009 16.366704161979754 42-43 16.15319389485585 31.81175805539853 35.57094403617863 16.46410401356699 44-45 16.931366988088488 31.409529211571186 34.642654566080544 17.016449234259785 46-47 15.790223742340032 32.492518170158185 34.15989739204788 17.557360695453898 48-49 17.12612483930867 32.866733323810884 33.69518640194258 16.311955434937868 50-51 16.2794033275961 33.40504876649455 32.86001147446931 17.45553643144005 52-53 16.930607543910163 33.73164411171897 33.803627987330835 15.534120357040024 54-55 16.7556645980661 33.771106941838646 32.847452734882374 16.625775725212875 56-57 17.452488031336138 34.51327433628318 32.16306397794864 15.871173654432033 58-59 16.29943090617248 35.196264409747556 32.61345396176857 15.890850722311397 60-61 15.754730820008803 34.6780108552149 32.36027578113539 17.206982543640898 62-63 16.002949852507374 36.06194690265487 30.9882005899705 16.94690265486726 64-65 16.688938381588716 35.96139569413512 30.809205642167782 16.540460282108388 66-67 16.724008975317876 34.89902767389678 31.17427075542259 17.202692595362752 68-69 16.510715363718685 35.73800181104739 31.587684877754302 16.163597947479627 70-71 16.62613981762918 35.54711246200608 30.759878419452885 17.066869300911854 72-73 16.93227091633466 35.70334048421698 30.140974563285322 17.22341403616304 74-75 16.47621988882026 36.82828906732551 29.956763434218654 16.73872760963558 76-77 16.70815183571873 36.1698817672682 30.336029869321717 16.78593652769135 78-79 17.29273539632633 38.77213304650009 27.35396326327983 16.58116829389376 80-81 19.2226148409894 39.2756183745583 22.314487632508833 19.18727915194346 82-83 17.84439685938615 41.113490364025694 22.466095645967165 18.576017130620983 84-85 18.79400613829211 41.361256544502616 21.700667990612025 18.144069326593247 86-87 17.997070670084216 41.17539362870743 21.933357744415964 18.894177956792387 88-89 19.121206479240367 40.27183019921802 21.671941910258795 18.935021411282815 90-91 17.327293318233295 40.82672706681767 22.76330690826727 19.082672706681766 92-93 19.055843408175015 40.54883899443485 21.972749952024564 18.42256764536557 94-95 17.803400429939416 40.94195817862028 22.47410592143834 18.780535470001954 96-97 18.499301536619438 40.151666334065055 22.37078427459589 18.97824785471962 98-99 18.584790565270435 41.01260675071167 22.61081740544937 17.791785278568522 100-101 17.430600387346676 41.48913277383258 22.207876049063913 18.87239078975683 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 319.0 1 459.5 2 571.0 3 502.0 4 401.5 5 172.5 6 6.0 7 6.5 8 5.5 9 5.5 10 8.0 11 12.5 12 9.5 13 4.0 14 6.5 15 12.0 16 13.0 17 18.0 18 21.5 19 15.5 20 9.0 21 13.0 22 20.0 23 23.0 24 27.5 25 27.0 26 22.5 27 24.0 28 27.0 29 27.0 30 29.0 31 35.0 32 39.0 33 45.5 34 54.5 35 64.0 36 79.5 37 82.0 38 100.0 39 114.5 40 113.0 41 127.0 42 132.5 43 135.0 44 122.5 45 113.5 46 114.5 47 89.0 48 76.0 49 82.5 50 73.5 51 66.0 52 58.0 53 51.5 54 45.5 55 36.0 56 34.0 57 34.0 58 30.0 59 28.0 60 28.0 61 24.5 62 24.5 63 25.0 64 24.0 65 17.0 66 15.0 67 15.5 68 11.0 69 8.5 70 12.5 71 14.5 72 10.5 73 8.0 74 5.5 75 4.5 76 3.5 77 2.5 78 3.0 79 2.0 80 1.0 81 1.0 82 1.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.575 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 178.0 32-33 157.0 34-35 56.0 36-37 29.0 38-39 20.0 40-41 19.0 42-43 10.0 44-45 20.0 46-47 9.0 48-49 13.0 50-51 14.0 52-53 9.0 54-55 13.0 56-57 21.0 58-59 18.0 60-61 18.0 62-63 22.0 64-65 28.0 66-67 26.0 68-69 27.0 70-71 23.0 72-73 24.0 74-75 26.0 76-77 33.0 78-79 350.0 80-81 27.0 82-83 30.0 84-85 38.0 86-87 47.0 88-89 35.0 90-91 42.0 92-93 45.0 94-95 51.0 96-97 52.0 98-99 68.0 100-101 2402.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.3 #Duplication Level Percentage of deduplicated Percentage of total 1 97.61570827489481 69.6 2 0.6311360448807855 0.8999999999999999 3 0.2805049088359046 0.6 4 0.3506311360448808 1.0 5 0.21037868162692847 0.75 6 0.10518934081346423 0.44999999999999996 7 0.21037868162692847 1.05 8 0.07012622720897616 0.4 9 0.10518934081346423 0.675 >10 0.21037868162692847 2.65 >50 0.1402524544179523 8.25 >100 0.07012622720897616 13.675 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 7.725 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 238 5.949999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 2.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 2.025 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78 1.95 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 1.9 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.8999999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.44999999999999996 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA 5 0.125 TruSeq Adapter, Index 4 (100% over 51bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 90 0.0 82.7375 1 CTTTTTT 90 0.0 82.7375 2 >>END_MODULE SRR13172458 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172458_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 32 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.073 34.0 34.0 40.0 31.0 41.0 2 36.35175 34.0 34.0 41.0 31.0 41.0 3 36.141 34.0 34.0 41.0 31.0 41.0 4 38.0555 37.0 37.0 41.0 35.0 41.0 5 38.0585 37.0 37.0 41.0 35.0 41.0 6 38.04525 37.0 37.0 41.0 35.0 41.0 7 37.98175 37.0 37.0 41.0 35.0 41.0 8 37.631 37.0 37.0 41.0 35.0 41.0 9 38.493 39.0 38.0 41.0 35.0 41.0 10-11 38.622749999999996 39.0 39.0 41.0 35.5 41.0 12-13 38.773375 39.0 39.0 41.0 37.0 41.0 14-15 38.367000000000004 39.0 37.0 41.0 35.5 41.0 16-17 37.648375 39.0 35.0 41.0 35.0 41.0 18-19 37.56075 39.0 35.0 41.0 35.0 41.0 20-21 37.450625 39.0 35.0 41.0 35.0 41.0 22-23 37.285125 38.0 35.0 40.5 34.5 41.0 24-25 37.098625 38.0 35.0 40.0 34.0 41.0 26-27 37.10875 38.0 35.0 40.5 34.0 41.0 28-29 36.759375000000006 37.5 35.0 40.0 33.0 41.0 30-31 36.923335702341134 38.0 35.0 40.5 33.0 41.0 32-33 36.98926517685457 38.0 35.0 41.0 33.0 41.0 34-35 37.10013732704138 38.0 35.0 41.0 33.0 41.0 36-37 36.99762732651253 38.0 35.0 40.5 33.0 41.0 38-39 36.82443717330341 38.0 35.0 40.0 33.0 41.0 40-41 36.78155152576778 37.0 35.0 40.0 33.0 41.0 42-43 36.65197814719883 37.0 35.0 40.0 33.0 41.0 44-45 36.528585151450116 36.5 35.0 40.0 33.0 41.0 46-47 36.39572929419469 36.0 35.0 40.0 33.0 41.0 48-49 36.20311467069927 36.0 35.0 40.0 33.0 41.0 50-51 35.85436434258082 35.0 35.0 39.0 32.0 40.5 52-53 36.015406107197684 35.0 35.0 39.5 33.0 40.5 54-55 36.207290774016855 35.0 35.0 40.0 33.0 41.0 56-57 36.20740860420436 35.0 35.0 40.0 33.0 41.0 58-59 36.017339195544096 35.0 35.0 40.0 33.0 41.0 60-61 35.79688235988727 35.0 35.0 39.0 32.5 41.0 62-63 35.72318335574269 35.0 35.0 39.0 32.5 41.0 64-65 35.649333514100405 35.0 35.0 38.5 33.0 41.0 66-67 35.369863449361475 35.0 34.5 37.5 32.0 40.5 68-69 35.16980534543322 35.0 34.5 37.0 31.5 40.0 70-71 34.96648327737562 35.0 34.0 37.0 31.5 40.0 72-73 34.80293766190587 35.0 34.0 36.0 31.0 39.0 74-75 34.73779502738485 35.0 34.0 36.0 31.0 39.0 76-77 34.52670918963415 35.0 34.0 35.5 31.0 38.0 78-79 34.50247092682832 35.0 34.0 35.5 31.0 38.0 80-81 34.4290011509204 35.0 34.0 36.0 31.0 37.0 82-83 34.05555418619177 35.0 34.0 35.5 30.5 37.0 84-85 34.06324647818707 35.0 34.0 35.0 31.0 36.5 86-87 33.85044332279605 35.0 34.0 35.0 31.0 36.0 88-89 33.849502390306924 35.0 34.0 35.0 31.0 36.0 90-91 33.65603907543571 35.0 33.5 35.0 30.5 35.5 92-93 33.6305153544661 35.0 33.5 35.0 30.0 35.0 94-95 33.60294864498484 35.0 33.5 35.0 31.0 35.0 96-97 33.55585748305815 35.0 33.0 35.0 30.5 35.0 98-99 33.54076569989728 35.0 33.0 35.0 30.5 35.0 100-101 33.28270550222577 34.5 32.5 35.0 30.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 5.0 22 3.0 23 6.0 24 12.0 25 10.0 26 16.0 27 23.0 28 19.0 29 36.0 30 49.0 31 43.0 32 87.0 33 108.0 34 232.0 35 719.0 36 767.0 37 1152.0 38 647.0 39 64.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.175 67.30000000000001 12.1 11.425 2 11.025 18.35 14.75 55.875 3 9.6 39.65 41.349999999999994 9.4 4 11.25 17.5 61.824999999999996 9.425 5 31.25 17.7 40.975 10.075000000000001 6 30.95 18.725 40.5 9.825000000000001 7 31.45 18.575 40.425 9.55 8 19.6 22.35 45.550000000000004 12.5 9 15.950000000000001 24.775 45.074999999999996 14.2 10-11 15.725 24.15 45.7 14.424999999999999 12-13 14.9375 23.8875 46.275 14.899999999999999 14-15 15.950000000000001 24.25 45.4375 14.3625 16-17 15.55 23.849999999999998 46.35 14.249999999999998 18-19 15.299999999999999 24.15 45.800000000000004 14.75 20-21 15.312500000000002 23.575 46.3125 14.799999999999999 22-23 15.4875 24.2 46.7625 13.55 24-25 15.0625 23.9125 46.5375 14.4875 26-27 15.475 24.1375 46.5 13.8875 28-29 15.45 23.674999999999997 46.137499999999996 14.7375 30-31 15.709395207303157 25.04120704957525 45.26435907189045 13.98503867123114 32-33 16.96741179639106 26.259089684890924 41.8394828979262 14.934015620791813 34-35 17.143257639472946 27.221754976170452 39.87945051864312 15.755536865713484 36-37 17.188389299943086 28.500284575981787 38.40352874217416 15.907797381900968 38-39 17.23840964547151 28.548873259652645 38.35223195062437 15.860485144251472 40-41 16.982496745262548 28.236655576450165 38.69521191957182 16.085635758715462 42-43 16.71750181554103 29.411764705882355 37.73420479302832 16.136528685548292 44-45 17.005113221329438 29.744338933528127 36.84441197954712 16.406135865595324 46-47 17.49155529446321 30.37156704361874 37.09795858422676 15.03891907769129 48-49 17.526230234963794 30.51573814097828 36.2494458401064 15.708585783951529 50-51 17.8045515394913 30.046110367395507 36.18920124944221 15.96013684367098 52-53 18.321866586897993 29.255160035895898 36.64373317379599 15.779240203410112 54-55 17.064743878624007 30.629412648340093 36.12738470782635 16.178458765209555 56-57 16.968325791855204 31.61387631975867 35.77677224736048 15.64102564102564 58-59 17.28582259287339 29.78013646702047 36.61865049279757 16.315390447308566 60-61 16.95380393352645 31.483457844183565 35.447476749504496 16.115261472785487 62-63 17.155582859775762 31.899861772385197 34.280448471816925 16.664106896022115 64-65 17.09242916860195 31.336120142436908 34.40161015637096 17.169840532590182 66-67 17.31068868806482 32.05048301651605 34.02929261452166 16.609535680897476 68-69 16.84227079729517 32.47365938040572 34.39220003145149 16.291869790847617 70-71 17.533291058972733 32.609384908053265 34.57514267596702 15.282181357006975 72-73 18.27338129496403 32.214228617106315 32.99760191846523 16.514788169464428 74-75 17.39059967585089 33.09562398703403 33.54943273905997 15.964343598055105 76-77 16.451137665739076 33.75347847438206 32.345719430348666 17.449664429530202 78-79 17.501520989657273 37.781383086595014 27.844250659095522 16.8728452646522 80-81 17.640918580375782 41.67536534446764 23.355949895615865 17.32776617954071 82-83 18.311722677957132 39.42842021698862 23.604128076210635 18.65572902884361 84-85 17.74753135842007 40.3789698425407 23.53883106485188 18.33466773418735 86-87 17.804154302670625 42.379282438629616 22.902616671162665 16.913946587537094 88-89 18.1793254729915 40.855497669317245 23.25198793528928 17.713188922401972 90-91 18.448227742115545 40.41306168015629 23.332403014233883 17.80630756349428 92-93 17.39621575826038 42.05026828579497 21.942953967805703 18.610561988138944 94-95 17.647058823529413 42.123385939741745 21.893830703012913 18.335724533715926 96-97 18.442622950819672 42.50585480093677 21.750585480093676 17.30093676814988 98-99 16.721409375933113 43.833980292624666 21.916990146312333 17.527620185129887 100-101 16.630196936542667 43.919974992185054 21.600500156298843 17.84932791497343 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 235.0 1 468.5 2 683.0 3 613.5 4 490.0 5 214.0 6 9.5 7 7.5 8 7.0 9 6.5 10 7.0 11 7.5 12 7.0 13 7.5 14 6.0 15 8.0 16 13.0 17 12.0 18 11.0 19 11.5 20 9.5 21 10.0 22 11.5 23 17.5 24 20.0 25 15.0 26 13.5 27 14.5 28 18.0 29 20.0 30 19.5 31 25.5 32 31.5 33 40.5 34 50.0 35 61.5 36 70.5 37 79.5 38 102.5 39 124.5 40 132.0 41 127.5 42 128.5 43 129.5 44 132.5 45 131.0 46 117.0 47 104.0 48 94.5 49 93.0 50 90.5 51 74.5 52 79.0 53 79.0 54 58.5 55 48.0 56 41.0 57 42.5 58 39.5 59 28.5 60 24.5 61 27.0 62 24.5 63 23.5 64 25.5 65 20.0 66 16.5 67 16.0 68 17.0 69 18.5 70 15.5 71 12.0 72 7.5 73 6.0 74 5.5 75 3.5 76 4.5 77 4.0 78 2.5 79 2.0 80 2.0 81 1.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.5 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014522218995062446 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 239.0 32-33 170.0 34-35 67.0 36-37 34.0 38-39 28.0 40-41 14.0 42-43 22.0 44-45 16.0 46-47 19.0 48-49 23.0 50-51 21.0 52-53 16.0 54-55 12.0 56-57 17.0 58-59 17.0 60-61 24.0 62-63 25.0 64-65 21.0 66-67 30.0 68-69 26.0 70-71 21.0 72-73 43.0 74-75 30.0 76-77 78.0 78-79 1062.0 80-81 31.0 82-83 16.0 84-85 18.0 86-87 29.0 88-89 30.0 90-91 27.0 92-93 26.0 94-95 31.0 96-97 32.0 98-99 41.0 100-101 1644.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.525 #Duplication Level Percentage of deduplicated Percentage of total 1 97.7015687705217 66.95 2 0.36483035388544327 0.5 3 0.2918642831083546 0.6 4 0.21889821233126594 0.6 5 0.2553812477198103 0.8750000000000001 6 0.18241517694272164 0.75 7 0.21889821233126594 1.05 8 0.1459321415541773 0.8 9 0.07296607077708865 0.44999999999999996 >10 0.3283473184968989 3.775 >50 0.07296607077708865 4.175 >100 0.1459321415541773 19.475 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 352 8.799999999999999 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 189 4.725 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 3.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113 2.825 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 2.35 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 1.825 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 1.175 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.475 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 35 1.3242243E-9 76.275 2 ATGGGCA 15 0.0014693693 76.274994 3 CATGGGT 15 0.0014693693 76.274994 2 CATGGGC 15 0.0014693693 76.274994 2 ACATGGG 95 0.0 72.26053 1 ACTTTTT 85 0.0 71.78824 1 CTTTTTT 85 0.0 71.78824 2 CATGGGA 35 1.0433436E-5 54.482143 2 >>END_MODULE Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621995 spots for SRR13172458.sra Written 1621995 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra Read 1621989 spots for SRR13172458.sra Written 1621989 spots for SRR13172458.sra SRR ids: ['SRR13172458.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_95nh3zq0 SRR13172458.sra spots: 32439786 blocks: [[1, 1621989], [1621990, 3243978], [3243979, 4865967], [4865968, 6487956], [6487957, 8109945], [8109946, 9731934], [9731935, 11353923], [11353924, 12975912], [12975913, 14597901], [14597902, 16219890], [16219891, 17841879], [17841880, 19463868], [19463869, 21085857], [21085858, 22707846], [22707847, 24329835], [24329836, 25951824], [25951825, 27573813], [27573814, 29195802], [29195803, 30817791], [30817792, 32439786]] SRR13172458 file size 6871743 SRR13172458 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172458 SRR13172458_1.fastq SRR13172458_2.fastq Input file: SRR13172458_1.fastq Paired file: SRR13172458_2.fastq trimmed: SRR13172458-trimmed-pair1.fastq, SRR13172458-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:11:30 2024 >> started Fri Dec 6 11:11:58 2024 >> done (27.476s) 32439786 read pairs processed; of these: 4 ( 0.00%) short read pairs filtered out after trimming by size control 35 ( 0.00%) empty read pairs filtered out after trimming by size control 32439747 (100.00%) read pairs available; of these: 2445752 ( 7.54%) trimmed read pairs available after processing 29993995 (92.46%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 0 0.00% 24 0 0.00% 25 1 0.00% 26 1 0.00% 27 3 0.00% 28 17 0.00% 29 662 0.00% 30 2920 0.01% 31 6198 0.02% 32 7813 0.02% 33 8848 0.03% 34 9697 0.03% 35 10342 0.03% 36 11250 0.03% 37 12459 0.04% 38 13989 0.04% 39 15816 0.05% 40 17726 0.05% 41 19825 0.06% 42 22766 0.07% 43 25905 0.08% 44 30148 0.09% 45 34742 0.11% 46 39627 0.12% 47 45484 0.14% 48 52641 0.16% 49 59201 0.18% 50 67823 0.21% 51 78238 0.24% 52 88027 0.27% 53 101667 0.31% 54 132667 0.41% 55 136266 0.42% 56 140157 0.43% 57 148228 0.46% 58 160508 0.49% 59 173468 0.53% 60 187556 0.58% 61 200871 0.62% 62 227637 0.70% 63 283165 0.87% 64 356547 1.10% 65 1346685 4.15% 66 2144751 6.61% 67 1139145 3.51% 68 509589 1.57% 69 342992 1.06% 70 305473 0.94% 71 295180 0.91% 72 287149 0.89% 73 280793 0.87% 74 289002 0.89% 75 283397 0.87% 76 287765 0.89% 77 324459 1.00% 78 515907 1.59% 79 426059 1.31% 80 394071 1.21% 81 375099 1.16% 82 428291 1.32% 83 454360 1.40% 84 475488 1.47% 85 483796 1.49% 86 576580 1.78% 87 605443 1.87% 88 944620 2.91% 89 8371093 25.81% 90 256179 0.79% 91 75226 0.23% 92 62600 0.19% 93 62133 0.19% 94 71322 0.22% 95 82856 0.26% 96 101326 0.31% 97 152355 0.47% 98 362070 1.12% 99 465925 1.44% 100 1415041 4.36% 101 4514620 13.92% 32439747 reads passed initial QC criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=38 prefix-density=0.00 prefix-fanout=1.0 sequence=ACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA criterion=fanout-score sequence-density=0.05 sequence-density-rank=5 fanout-score=277.91 fanout-score-rank=1 prefix-density=0.41 prefix-fanout=35.0 sequence=CCCATGTACTCTGCGTTGATACCACTG criterion=sequence-density sequence-density=0.16 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=48 prefix-density=0.00 prefix-fanout=1.0 sequence=GTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=47 fanout-score=143.67 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=12.7 sequence=CCGCCGCCGCCCTACCACCACTAAGATCCATCACTCTCTTTAGTTGGGAGCACAGAATGTGATCGAAAAGACGTGAGCCGACTGTCTGAAGATGACGAGCCTTTGATTCTCCTCCCGTGCGTCGCCGCCAACCTAGGGCCAAGATCGAATAATGCAGACTGCTCCCTTCAGTTTATCTTACAGCTTATAATTTGTATTCGCCCAGTCTTAGCGTGGAAGTGCTGGCATTGTTAGCGTCGTCCCTGTGTTACTGTAAAACCATTTAGTAACTTTGGAAATGGAAGTAAAATCTGTTCACCCT SRR13172458 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:13:11 Started mapping on | Dec 06 11:13:11 Finished on | Dec 06 11:27:19 Mapping speed, Million of reads per hour | 137.72 Number of input reads | 32439747 Average input read length | 165 UNIQUE READS: Uniquely mapped reads number | 16459244 Uniquely mapped reads % | 50.74% Average mapped length | 164.91 Number of splices: Total | 4070103 Number of splices: Annotated (sjdb) | 3507964 Number of splices: GT/AG | 3921504 Number of splices: GC/AG | 53756 Number of splices: AT/AC | 3170 Number of splices: Non-canonical | 91673 Mismatch rate per base, % | 0.26% Deletion rate per base | 0.02% Deletion average length | 1.39 Insertion rate per base | 0.00% Insertion average length | 1.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2382416 % of reads mapped to multiple loci | 7.34% Number of reads mapped to too many loci | 195965 % of reads mapped to too many loci | 0.60% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 40.61% % of reads unmapped: other | 0.70% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 17014897 17014897 17014897 N_multimapping 2382416 2382416 2382416 N_noFeature 754074 10601438 6212782 N_ambiguous 468414 30928 43414 UnstrandedReadsAssigned:15236756 PositiveStrandReadsAssigned:5826878 NegativeStrandReadsAssigned:10203048 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=76 echo kmer=71 SRR13172458 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172458-trimmed-pair1.fastq SRR13172458-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,439,747 reads, 25,414,981 reads pseudoaligned [quant] estimated average fragment length: 138.386 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,194 rounds 52973 SRR13172458.ke.tsv 35125 SRR13172458.se.tsv 88098 total ==> SRR13172458.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 798.65 0 0 PNS24247 1044 906.614 0 0 PNS24249 1928 1790.61 0 0 PNS24246 1044 906.614 0 0 PNS24248 1044 906.614 0 0 PNS24244 1471 1333.61 909 42.9995 PNS24243 293 156.859 0 0 KQK14069 1603 1465.61 170 7.31744 KQK14071 474 337.188 0 0 ==> SRR13172458.se.tsv <== BRADI_1g14170v3 158 BRADI_1g53295v3 17 BRADI_1g59795v3 912 BRADI_1g07683v3 0 BRADI_1g00485v3 5 BRADI_1g20270v3 1043 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 734 BRADI_1g48960v3 0 SRR13172458 completed mapping pipeline successfully