Starting /dee2/code/volunteer_pipeline.sh SRR13172459 current disk space = 1551443161088 free memory = 1604061904 SRR13172459 SRAfilesize 72fda3492ddb4f3955634b45170e40b8 SRR13172459.sra SRR13172459.sra file validated SRR13172459 is paired end SRR13172459 is conventional basespace SRR13172459 read1 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172459_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 29 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.572 34.0 34.0 34.0 31.0 40.0 2 34.9025 34.0 34.0 34.0 31.0 41.0 3 34.91175 34.0 34.0 34.0 31.0 41.0 4 37.45275 37.0 37.0 37.0 35.0 41.0 5 37.2915 37.0 37.0 37.0 35.0 41.0 6 37.244 37.0 37.0 37.0 35.0 41.0 7 37.4185 37.0 37.0 37.0 35.0 41.0 8 37.4185 37.0 37.0 37.0 35.0 41.0 9 38.82675 39.0 39.0 39.0 37.0 41.0 10-11 38.771375 39.0 39.0 39.0 37.0 41.0 12-13 38.730625 39.0 39.0 39.0 37.0 41.0 14-15 39.023625 40.0 37.0 41.0 36.5 41.0 16-17 38.48925 40.0 35.0 41.0 35.0 41.0 18-19 38.153999999999996 40.0 35.0 41.0 35.0 41.0 20-21 37.918499999999995 40.0 35.0 41.0 35.0 41.0 22-23 37.91175 39.5 35.0 41.0 35.0 41.0 24-25 37.83175 39.0 35.0 41.0 35.0 41.0 26-27 37.809625 39.0 35.0 41.0 35.0 41.0 28-29 37.756625 39.0 35.0 41.0 35.0 41.0 30-31 37.50048827524346 39.0 35.0 41.0 34.0 41.0 32-33 37.460138421471974 39.0 35.0 41.0 33.0 41.0 34-35 37.50057137411197 39.5 35.0 41.0 33.0 41.0 36-37 37.67572112589997 40.0 35.0 41.0 34.5 41.0 38-39 37.64088419461153 39.0 35.0 41.0 35.0 41.0 40-41 37.58444487781759 39.0 35.0 41.0 34.0 41.0 42-43 37.463993217184935 39.0 35.0 41.0 34.0 41.0 44-45 37.515699152864784 39.0 35.0 41.0 34.0 41.0 46-47 37.43702261366556 39.0 35.0 41.0 33.0 41.0 48-49 37.28582137022641 38.5 35.0 41.0 33.0 41.0 50-51 37.25778933114171 38.5 35.0 41.0 33.0 41.0 52-53 37.17255546952443 38.0 35.0 41.0 33.0 41.0 54-55 37.17724463031267 38.0 35.0 41.0 33.0 41.0 56-57 37.08242946260442 38.0 35.0 40.0 33.0 41.0 58-59 36.93141859884051 37.0 35.0 40.0 33.0 41.0 60-61 36.78230902689995 37.0 35.0 40.0 33.0 41.0 62-63 36.48840247456073 36.0 35.0 40.0 33.0 41.0 64-65 36.29222907648196 36.0 35.0 40.0 33.0 41.0 66-67 36.1057616086659 35.0 35.0 39.0 33.0 41.0 68-69 35.75212294296017 35.0 35.0 39.0 32.5 41.0 70-71 35.49124012404329 35.0 35.0 38.5 32.0 40.0 72-73 35.15543782946317 35.0 35.0 37.0 31.5 40.0 74-75 34.96422326736898 35.0 34.5 37.0 31.0 39.0 76-77 34.22605423552919 35.0 33.5 36.0 30.5 38.5 78-79 34.79646098059767 35.0 34.0 36.0 31.5 39.0 80-81 34.716036010834415 35.0 34.0 36.0 32.0 37.5 82-83 34.62575964694017 35.0 34.5 35.5 32.0 37.0 84-85 34.37986274562997 35.0 34.0 35.0 32.0 37.0 86-87 34.16445030874499 35.0 34.0 35.0 31.0 36.0 88-89 33.93325670458712 35.0 34.0 35.0 31.0 36.0 90-91 34.069340662703674 35.0 34.0 35.0 31.5 36.0 92-93 33.89919262984544 35.0 34.0 35.0 31.0 35.5 94-95 33.79576810822968 35.0 34.0 35.0 31.0 35.0 96-97 33.94943985261378 35.0 34.0 35.0 31.0 35.0 98-99 33.935170983089826 35.0 34.0 35.0 31.5 35.0 100-101 33.45136102391459 34.5 33.0 35.0 30.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 4.0 22 4.0 23 3.0 24 1.0 25 10.0 26 15.0 27 17.0 28 19.0 29 24.0 30 35.0 31 53.0 32 47.0 33 97.0 34 211.0 35 717.0 36 596.0 37 1110.0 38 899.0 39 135.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.45 53.300000000000004 17.5 16.75 2 14.274999999999999 27.55 22.225 35.949999999999996 3 12.35 28.549999999999997 45.375 13.725000000000001 4 13.925 27.150000000000002 45.475 13.450000000000001 5 15.398141170560162 27.65636774679729 43.607133885958305 13.33835719668425 6 13.65 28.375 43.025000000000006 14.95 7 15.85 27.474999999999998 43.0 13.675 8 13.65 27.775 44.625 13.950000000000001 9 13.950000000000001 27.675 45.0 13.375 10-11 13.6875 28.237499999999997 44.25 13.825000000000001 12-13 14.399999999999999 28.9125 42.775 13.9125 14-15 14.45 28.525 43.6 13.425 16-17 13.925 28.1 43.974999999999994 14.000000000000002 18-19 14.424999999999999 28.787499999999998 43.05 13.737499999999999 20-21 13.100000000000001 29.549999999999997 43.0 14.35 22-23 13.4125 29.1375 44.0625 13.3875 24-25 13.487499999999999 28.712500000000002 44.074999999999996 13.725000000000001 26-27 13.900000000000002 29.099999999999998 43.0 14.000000000000002 28-29 14.0375 29.462500000000002 42.425000000000004 14.075 30-31 13.86990635282207 30.397367754998733 42.20450518855986 13.528220703619336 32-33 14.524472741742937 31.55590927178671 40.0716275368086 13.84799044966176 34-35 14.724010409532939 33.11875085604712 37.20038350910834 14.956855225311601 36-37 16.078376876042245 32.768204558087824 35.8532518065592 15.300166759310729 38-39 15.456576375314157 33.87321977101369 36.19100809829657 14.479195755375592 40-41 15.44498948843728 34.35178696566223 35.09460406447092 15.108619481429573 42-43 14.92179794279273 34.6625334648443 35.52205157108638 14.893617021276595 44-45 15.39550021225414 34.07386444035659 34.6540257535022 15.876609593887082 46-47 14.644886363636363 35.12784090909091 34.61647727272727 15.610795454545453 48-49 14.97432972047918 35.1968054763263 34.42669709070165 15.40216771249287 50-51 14.763948497854077 35.493562231759654 34.062947067238916 15.679542203147353 52-53 14.922591743119265 36.49655963302752 33.758600917431195 14.822247706422017 54-55 15.01799856011519 36.70266378689705 32.858171346292295 15.421166306695463 56-57 15.678333815446917 36.780445472953424 33.19352039340468 14.347700318194967 58-59 14.888048851410293 38.005234079674324 32.04419889502763 15.062518173887756 60-61 15.156819839533187 38.00145878920496 31.918307804522243 14.923413566739608 62-63 15.483870967741936 38.10850439882698 31.906158357771265 14.501466275659824 64-65 15.03239104829211 38.206713780918726 31.905182567726737 14.855712603062427 66-67 15.005184417123388 37.99437120426604 32.024885202192266 14.975559176418308 68-69 14.980544747081712 38.74588446572883 30.903920981741994 15.369649805447471 70-71 14.903556359252562 38.71308016877637 31.615430982519587 14.767932489451477 72-73 14.959671282909756 40.11565971693806 30.81722721047025 14.107441789681936 74-75 14.334103156274056 40.47729022324865 30.469591993841416 14.719014626635873 76-77 15.308027380211575 40.63472308649658 29.604853764779094 14.452395768512757 78-79 15.413970466235275 43.47104695536751 25.866932138709142 15.248050439688072 80-81 16.01143674052895 45.300214438884915 22.301644031451037 16.386704789135095 82-83 16.53985507246377 44.8731884057971 21.594202898550723 16.992753623188406 84-85 16.293166789125642 44.74650991917707 21.877296105804554 17.08302718589273 86-87 14.711914972962894 45.90714152526571 22.300950960283423 17.07999254148797 88-89 16.112580279561765 46.12769172648281 21.68492633169626 16.074801662259162 90-91 15.99537483137406 45.55791096550395 21.3528618230873 17.093852380034686 92-93 15.753154574132491 44.83438485804416 22.397476340694006 17.014984227129336 94-95 15.479813349563806 45.60762832217488 22.134307161696086 16.778251166565227 96-97 17.081329709387415 44.76270123353544 21.639138615931426 16.516830441145725 98-99 16.174582798459564 44.929396662387674 21.287976037655113 17.60804450149765 100-101 15.932584269662922 45.752808988764045 19.93258426966292 18.382022471910112 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 387.0 1 513.0 2 591.5 3 503.5 4 404.5 5 178.5 6 13.0 7 10.0 8 9.0 9 12.5 10 10.0 11 9.5 12 10.0 13 8.0 14 9.0 15 14.0 16 15.0 17 16.5 18 19.0 19 18.0 20 18.0 21 21.0 22 25.0 23 28.0 24 31.0 25 28.5 26 33.5 27 37.5 28 35.0 29 37.5 30 37.5 31 40.0 32 43.0 33 51.0 34 66.0 35 75.0 36 89.0 37 98.5 38 105.0 39 107.5 40 114.5 41 117.5 42 108.0 43 106.0 44 101.5 45 104.0 46 95.5 47 84.0 48 74.5 49 71.5 50 72.5 51 61.5 52 56.0 53 50.0 54 36.0 55 29.0 56 26.0 57 25.5 58 22.0 59 16.5 60 18.5 61 20.0 62 19.0 63 14.0 64 11.5 65 9.5 66 9.0 67 12.0 68 10.0 69 4.0 70 4.5 71 6.0 72 5.5 73 5.0 74 4.5 75 3.5 76 1.5 77 0.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.475 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.01326259946949602 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 191.0 32-33 139.0 34-35 65.0 36-37 22.0 38-39 11.0 40-41 18.0 42-43 17.0 44-45 13.0 46-47 15.0 48-49 10.0 50-51 9.0 52-53 14.0 54-55 14.0 56-57 19.0 58-59 14.0 60-61 15.0 62-63 16.0 64-65 19.0 66-67 30.0 68-69 23.0 70-71 33.0 72-73 33.0 74-75 40.0 76-77 36.0 78-79 379.0 80-81 36.0 82-83 36.0 84-85 45.0 86-87 31.0 88-89 50.0 90-91 58.0 92-93 67.0 94-95 70.0 96-97 67.0 98-99 50.0 100-101 2295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.25 #Duplication Level Percentage of deduplicated Percentage of total 1 97.79783393501805 67.72500000000001 2 0.36101083032490977 0.5 3 0.2888086642599278 0.6 4 0.4693140794223827 1.3 5 0.2527075812274368 0.8750000000000001 6 0.18050541516245489 0.75 7 0.18050541516245489 0.8750000000000001 8 0.036101083032490974 0.2 9 0.036101083032490974 0.22499999999999998 >10 0.18050541516245489 2.45 >50 0.1444043321299639 8.200000000000001 >100 0.07220216606498195 16.3 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 346 8.649999999999999 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 306 7.6499999999999995 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98 2.45 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 2.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 77 1.925 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 1.5 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38 0.95 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.65 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTTT 50 0.0 87.73417 1 CTTTTTT 55 0.0 79.75834 2 >>END_MODULE SRR13172459 read2 length is 30-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13172459_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 30-101 %GC 29 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0805 34.0 33.0 41.0 31.0 41.0 2 36.36975 34.0 34.0 41.0 31.0 41.0 3 36.17025 34.0 34.0 41.0 31.0 41.0 4 38.1195 37.0 37.0 41.0 35.0 41.0 5 38.12775 37.0 37.0 41.0 35.0 41.0 6 38.13925 37.0 37.0 41.0 35.0 41.0 7 38.05975 37.0 37.0 41.0 35.0 41.0 8 37.77725 37.0 37.0 41.0 35.0 41.0 9 38.667 39.0 39.0 41.0 36.0 41.0 10-11 38.8015 39.0 39.0 41.0 37.0 41.0 12-13 38.8835 39.0 39.0 41.0 37.0 41.0 14-15 38.306375 39.0 37.0 41.0 36.0 41.0 16-17 37.447625 39.0 35.0 41.0 35.0 41.0 18-19 37.34825 38.0 35.0 41.0 35.0 41.0 20-21 37.205875000000006 38.0 35.0 41.0 35.0 41.0 22-23 37.07575 37.0 35.0 40.5 34.5 41.0 24-25 36.89275 36.5 35.0 40.0 34.0 41.0 26-27 36.978125 36.5 35.0 40.0 34.5 41.0 28-29 36.61325 36.0 35.0 40.0 33.0 41.0 30-31 36.62833442391583 36.0 35.0 40.0 33.0 41.0 32-33 36.7962514225257 37.5 35.0 41.0 33.0 41.0 34-35 36.99367872279092 38.0 35.0 41.0 33.0 41.0 36-37 36.978594936546756 38.0 35.0 40.5 33.0 41.0 38-39 36.77926331443281 37.5 35.0 40.0 33.0 41.0 40-41 36.829437452256684 37.0 35.0 40.0 33.0 41.0 42-43 36.77669110737216 37.0 35.0 40.0 33.0 41.0 44-45 36.514433016935875 36.0 35.0 40.0 33.0 41.0 46-47 36.38554649563933 36.0 35.0 40.0 33.0 41.0 48-49 36.32917807524592 35.5 35.0 40.0 33.0 41.0 50-51 35.99668723222834 35.0 35.0 39.0 32.5 40.5 52-53 36.17707887915789 35.0 35.0 39.5 33.0 40.5 54-55 36.3929090367063 35.0 35.0 40.0 33.0 41.0 56-57 36.42955906010054 35.0 35.0 40.0 33.0 41.0 58-59 36.172640491480884 35.0 35.0 40.0 33.0 41.0 60-61 35.994835491313054 35.0 35.0 39.5 33.0 41.0 62-63 35.84428219747987 35.0 35.0 39.0 33.0 41.0 64-65 35.779778638498286 35.0 35.0 39.0 33.0 41.0 66-67 35.41604711875199 35.0 35.0 38.0 32.5 40.5 68-69 35.388808462673765 35.0 35.0 37.0 32.5 40.0 70-71 35.15631825801317 35.0 35.0 37.0 32.0 40.0 72-73 35.05451695864461 35.0 35.0 36.5 32.0 39.0 74-75 34.851807289358604 35.0 34.0 36.0 31.5 39.0 76-77 34.706687898306754 35.0 34.0 35.5 31.5 38.5 78-79 34.73452253207439 35.0 34.0 35.5 31.5 38.0 80-81 34.6831407435761 35.0 34.0 36.0 32.0 37.5 82-83 34.19492650823679 35.0 34.0 35.5 31.0 37.0 84-85 34.22476169459405 35.0 34.0 35.0 31.0 37.0 86-87 34.157378070981295 35.0 34.0 35.0 31.0 36.0 88-89 34.04635143007628 35.0 34.0 35.0 31.0 36.0 90-91 33.80902534327672 35.0 34.0 35.0 31.0 36.0 92-93 33.707311571221965 35.0 33.5 35.0 31.0 35.5 94-95 33.644931849586115 35.0 33.0 35.0 31.0 35.0 96-97 33.696859399391045 35.0 34.0 35.0 31.0 35.0 98-99 33.67641255804823 35.0 33.0 35.0 31.0 35.0 100-101 33.421851245123804 34.5 33.0 35.0 30.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 3.0 22 6.0 23 3.0 24 6.0 25 7.0 26 11.0 27 19.0 28 23.0 29 30.0 30 40.0 31 49.0 32 67.0 33 113.0 34 235.0 35 787.0 36 750.0 37 1115.0 38 659.0 39 74.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.649999999999999 69.39999999999999 11.325000000000001 10.625 2 9.9 20.225 15.35 54.525 3 9.9 35.425000000000004 45.35 9.325 4 9.55 19.5 61.275 9.675 5 24.875 19.35 46.425 9.35 6 24.575 18.925 46.5 10.0 7 24.4 20.150000000000002 47.099999999999994 8.35 8 15.975 21.85 50.125 12.049999999999999 9 13.5 24.224999999999998 50.475 11.799999999999999 10-11 13.475000000000001 23.400000000000002 50.5875 12.537499999999998 12-13 13.475000000000001 23.45 50.175000000000004 12.9 14-15 14.075 23.175 50.287499999999994 12.4625 16-17 13.3125 23.75 50.449999999999996 12.4875 18-19 14.475 23.7875 49.3875 12.35 20-21 13.15 23.95 50.6625 12.237499999999999 22-23 12.7875 23.5 51.0625 12.65 24-25 13.212499999999999 24.775 50.175000000000004 11.837499999999999 26-27 13.5125 23.6125 50.31250000000001 12.562499999999998 28-29 13.2625 24.425 50.025 12.2875 30-31 13.562112194504241 24.920856021273902 48.879321261238445 12.637710522983411 32-33 14.151585249693834 26.65668798475983 45.652469723771944 13.539257041774391 34-35 14.097798112668 28.55304546754361 43.49442379182156 13.854732627966829 36-37 15.178962746530313 28.89700511322133 40.87655222790358 15.047479912344777 38-39 15.555883220289 29.07696844588617 40.81391919787673 14.553229135948097 40-41 15.653337297458005 28.6903523115802 40.850304742084134 14.806005648877656 42-43 15.244358092960693 29.63682558660888 40.00896726946645 15.10984905096398 44-45 15.433212996389893 30.339951865222623 40.26774969915764 13.959085439229844 46-47 15.715579710144928 30.60084541062802 39.76449275362319 13.919082125603865 48-49 14.801882495825112 31.182632457871566 39.5475937452558 14.467891301047517 50-51 15.4210606755311 30.490600641907385 39.26333486168424 14.825003820877273 52-53 15.551120663186982 30.764507215228736 38.88547743322076 14.798894688363525 54-55 15.334872979214781 31.193225558121636 38.61431870669746 14.857582755966128 56-57 15.597464832277014 31.844179935074973 38.59947441644767 13.95888081620034 58-59 15.018656716417912 31.529850746268657 38.930348258706466 14.521144278606965 60-61 15.194622479287165 32.218227294044084 38.26793809598249 14.31921213068626 62-63 15.861311578286792 32.75807969877628 37.82554126137433 13.555067461562597 64-65 14.992101105845181 32.796208530805686 37.788309636650865 14.423380726698262 66-67 15.863389722310883 33.56208107245451 36.46664538780721 14.107883817427386 68-69 15.157860824742267 33.40850515463917 36.920103092783506 14.513530927835053 70-71 14.88288874430709 34.791802212101494 36.125569290826284 14.199739752765128 72-73 15.76257213520198 34.85572959604287 35.31739488870569 14.064303380049465 74-75 15.442404006677796 34.24040066777963 36.4440734557596 13.873121869782972 76-77 15.589674371520163 34.75620043867049 35.98785220178843 13.666272988020921 78-79 15.595951249741788 38.1739310059905 30.61350960545342 15.616608138814295 80-81 18.169796350171914 42.634223750330605 22.90399365247289 16.2919862470246 82-83 17.59656652360515 44.58154506437768 22.15665236051502 15.665236051502147 84-85 16.711809317443123 43.824485373781144 23.45612134344529 16.007583965330443 86-87 16.90914081800714 43.61789733735932 22.15207246774636 17.32088937688718 88-89 16.852367688022284 42.45125348189415 23.426183844011142 17.270194986072422 90-91 17.333333333333336 43.63120567375886 22.01418439716312 17.02127659574468 92-93 16.37410071942446 43.539568345323744 23.33812949640288 16.74820143884892 94-95 17.238320520402127 42.814902424600824 22.619751626256654 17.327025428740388 96-97 18.07703973309069 42.37185319987868 22.899605702153472 16.65150136487716 98-99 16.5015479876161 43.56037151702786 23.25077399380805 16.687306501547987 100-101 17.055771725032425 43.28793774319066 22.63294422827497 17.023346303501945 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 284.0 1 572.0 2 809.5 3 708.0 4 567.5 5 241.0 6 6.0 7 10.0 8 10.5 9 7.0 10 5.5 11 5.5 12 6.0 13 7.5 14 8.5 15 9.0 16 11.5 17 10.5 18 8.0 19 11.5 20 13.5 21 14.0 22 13.0 23 12.5 24 22.5 25 31.0 26 28.0 27 23.0 28 22.0 29 27.0 30 27.5 31 29.0 32 40.0 33 51.5 34 49.0 35 57.5 36 82.0 37 88.0 38 95.5 39 108.5 40 112.0 41 113.0 42 114.5 43 112.0 44 110.0 45 114.5 46 105.5 47 96.0 48 80.0 49 66.5 50 81.0 51 81.0 52 62.0 53 50.5 54 46.5 55 46.0 56 38.5 57 26.5 58 24.0 59 20.5 60 22.5 61 27.5 62 22.0 63 19.5 64 16.0 65 11.0 66 12.0 67 8.5 68 7.0 69 8.5 70 7.0 71 5.5 72 5.5 73 4.5 74 2.5 75 3.0 76 2.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014943215780035862 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 256.0 32-33 219.0 34-35 90.0 36-37 35.0 38-39 31.0 40-41 16.0 42-43 27.0 44-45 9.0 46-47 17.0 48-49 23.0 50-51 15.0 52-53 13.0 54-55 10.0 56-57 17.0 58-59 23.0 60-61 7.0 62-63 21.0 64-65 30.0 66-67 31.0 68-69 30.0 70-71 35.0 72-73 40.0 74-75 29.0 76-77 72.0 78-79 1005.0 80-81 30.0 82-83 20.0 84-85 22.0 86-87 24.0 88-89 36.0 90-91 25.0 92-93 39.0 94-95 44.0 96-97 37.0 98-99 36.0 100-101 1586.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.1 #Duplication Level Percentage of deduplicated Percentage of total 1 97.54428341384863 60.575 2 0.322061191626409 0.4 3 0.36231884057971014 0.675 4 0.36231884057971014 0.8999999999999999 5 0.12077294685990338 0.375 6 0.20128824476650561 0.75 7 0.24154589371980675 1.05 8 0.08051529790660225 0.4 9 0.12077294685990338 0.675 >10 0.36231884057971014 3.4000000000000004 >50 0.08051529790660225 3.375 >100 0.20128824476650561 27.425 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 11.95 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 226 5.65 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 3.8249999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 3.4750000000000005 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 2.5250000000000004 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 1.975 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 1.4000000000000001 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.8 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.5499999999999999 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.325 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.3 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.27499999999999997 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.25 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.22499999999999998 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.17500000000000002 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.15 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTC 15 0.0021966936 68.925 3 ATGGGGT 15 0.0021966936 68.925 3 ACATGGG 60 0.0 68.925 1 ACTTTTT 160 0.0 68.924995 1 CATGGGT 25 2.5841073E-6 68.924995 2 CTTTTTT 160 0.0 68.924995 2 CATGGGG 35 2.5503687E-7 59.07857 2 >>END_MODULE Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389748 spots for SRR13172459.sra Written 1389748 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra Read 1389737 spots for SRR13172459.sra Written 1389737 spots for SRR13172459.sra SRR ids: ['SRR13172459.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_thpb93oe SRR13172459.sra spots: 27794751 blocks: [[1, 1389737], [1389738, 2779474], [2779475, 4169211], [4169212, 5558948], [5558949, 6948685], [6948686, 8338422], [8338423, 9728159], [9728160, 11117896], [11117897, 12507633], [12507634, 13897370], [13897371, 15287107], [15287108, 16676844], [16676845, 18066581], [18066582, 19456318], [19456319, 20846055], [20846056, 22235792], [22235793, 23625529], [23625530, 25015266], [25015267, 26405003], [26405004, 27794751]] SRR13172459 file size 5845318 SRR13172459 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13172459 SRR13172459_1.fastq SRR13172459_2.fastq Input file: SRR13172459_1.fastq Paired file: SRR13172459_2.fastq trimmed: SRR13172459-trimmed-pair1.fastq, SRR13172459-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:12:32 2024 >> started Fri Dec 6 11:13:13 2024 >> done (41.397s) 27794751 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 27 ( 0.00%) empty read pairs filtered out after trimming by size control 27794724 (100.00%) read pairs available; of these: 2389251 ( 8.60%) trimmed read pairs available after processing 25405473 (91.40%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 27 3 0.00% 28 11 0.00% 29 643 0.00% 30 2452 0.01% 31 4827 0.02% 32 5685 0.02% 33 6607 0.02% 34 7243 0.03% 35 7443 0.03% 36 8500 0.03% 37 9380 0.03% 38 10299 0.04% 39 12058 0.04% 40 13543 0.05% 41 15447 0.06% 42 17837 0.06% 43 20430 0.07% 44 24072 0.09% 45 28343 0.10% 46 33152 0.12% 47 38991 0.14% 48 45188 0.16% 49 51578 0.19% 50 59678 0.21% 51 69257 0.25% 52 77777 0.28% 53 87787 0.32% 54 112216 0.40% 55 122539 0.44% 56 130774 0.47% 57 139761 0.50% 58 153981 0.55% 59 166903 0.60% 60 181025 0.65% 61 195916 0.70% 62 220408 0.79% 63 268095 0.96% 64 332151 1.20% 65 1170695 4.21% 66 1868234 6.72% 67 1028308 3.70% 68 460811 1.66% 69 304120 1.09% 70 266996 0.96% 71 258497 0.93% 72 252114 0.91% 73 248360 0.89% 74 249022 0.90% 75 250610 0.90% 76 258669 0.93% 77 284816 1.02% 78 448442 1.61% 79 396502 1.43% 80 362640 1.30% 81 345488 1.24% 82 398926 1.44% 83 423152 1.52% 84 440567 1.59% 85 442570 1.59% 86 504266 1.81% 87 514209 1.85% 88 740506 2.66% 89 6930872 24.94% 90 234460 0.84% 91 66408 0.24% 92 52341 0.19% 93 52838 0.19% 94 59081 0.21% 95 69173 0.25% 96 83376 0.30% 97 125431 0.45% 98 299351 1.08% 99 379613 1.37% 100 1136516 4.09% 101 3704744 13.33% 27794724 reads passed initial QC criterion=sequence-density sequence-density=0.08 sequence-density-rank=1 fanout-score=5.92 fanout-score-rank=23 prefix-density=0.18 prefix-fanout=2.7 sequence=GCTAGCTAGCTGGGCGGCGATGGTGGGTGCATGCTTGCAGTGCAGTTGTCCTAGATCCTGGATCGATCCTCATTCCTCATGGTCGCTGGTGTGTGGCTCTAGTTGCAGGTGCAGCAGGAGCAGCCGCAGGCGGTGCCGCACTTG criterion=fanout-score sequence-density=0.04 sequence-density-rank=6 fanout-score=256.07 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=33.1 sequence=CCCATGTACTCTGCGTTGATACCAC criterion=sequence-density sequence-density=0.10 sequence-density-rank=1 fanout-score=1.54 fanout-score-rank=15 prefix-density=0.02 prefix-fanout=1.5 sequence=ACATGGGGAAGA criterion=fanout-score sequence-density=0.05 sequence-density-rank=18 fanout-score=62.31 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=12.3 sequence=GAAGAAGAAGAAA SRR13172459 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:15:03 Started mapping on | Dec 06 11:15:03 Finished on | Dec 06 11:28:25 Mapping speed, Million of reads per hour | 124.76 Number of input reads | 27794724 Average input read length | 164 UNIQUE READS: Uniquely mapped reads number | 12246921 Uniquely mapped reads % | 44.06% Average mapped length | 164.93 Number of splices: Total | 2752767 Number of splices: Annotated (sjdb) | 2313991 Number of splices: GT/AG | 2632463 Number of splices: GC/AG | 35273 Number of splices: AT/AC | 1916 Number of splices: Non-canonical | 83115 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.48 Insertion rate per base | 0.01% Insertion average length | 1.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2124703 % of reads mapped to multiple loci | 7.64% Number of reads mapped to too many loci | 172051 % of reads mapped to too many loci | 0.62% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 46.84% % of reads unmapped: other | 0.84% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 16163142 16163142 16163142 N_multimapping 2124703 2124703 2124703 N_noFeature 586434 7444890 5083721 N_ambiguous 357258 25606 30904 UnstrandedReadsAssigned:11303229 PositiveStrandReadsAssigned:4776425 NegativeStrandReadsAssigned:7132296 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=75 echo kmer=71 SRR13172459 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13172459-trimmed-pair1.fastq SRR13172459-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,794,724 reads, 21,727,641 reads pseudoaligned [quant] estimated average fragment length: 134.594 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,123 rounds 52973 SRR13172459.ke.tsv 35125 SRR13172459.se.tsv 88098 total ==> SRR13172459.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 802.439 0 0 PNS24247 1044 910.406 0 0 PNS24249 1928 1794.41 0 0 PNS24246 1044 910.406 0 0 PNS24248 1044 910.406 0 0 PNS24244 1471 1337.41 427 25.3885 PNS24243 293 160.579 0 0 KQK14069 1603 1469.41 88 4.76227 KQK14071 474 341.077 0 0 ==> SRR13172459.se.tsv <== BRADI_1g14170v3 80 BRADI_1g53295v3 0 BRADI_1g59795v3 989 BRADI_1g07683v3 0 BRADI_1g00485v3 48 BRADI_1g20270v3 1333 BRADI_1g74790v3 6 BRADI_1g09890v3 0 BRADI_1g77505v3 213 BRADI_1g48960v3 0 SRR13172459 completed mapping pipeline successfully