Starting /dee2/code/volunteer_pipeline.sh SRR18694423 current disk space = 1526092857344 free memory = 1602343796 SRR18694423 SRAfilesize 82e8aa979ef77340cac9272997a301dc SRR18694423.sra SRR18694423.sra file validated SRR18694423 is paired end SRR18694423 is conventional basespace SRR18694423 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR18694423_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.046 37.0 37.0 37.0 37.0 37.0 2 36.01225 37.0 37.0 37.0 37.0 37.0 3 36.3865 37.0 37.0 37.0 37.0 37.0 4 36.5775 37.0 37.0 37.0 37.0 37.0 5 36.5375 37.0 37.0 37.0 37.0 37.0 6 36.507 37.0 37.0 37.0 37.0 37.0 7 36.535 37.0 37.0 37.0 37.0 37.0 8 36.5785 37.0 37.0 37.0 37.0 37.0 9 36.675 37.0 37.0 37.0 37.0 37.0 10-14 36.6011 37.0 37.0 37.0 37.0 37.0 15-19 36.6274 37.0 37.0 37.0 37.0 37.0 20-24 36.647 37.0 37.0 37.0 37.0 37.0 25-29 36.6392 37.0 37.0 37.0 37.0 37.0 30-34 36.5964 37.0 37.0 37.0 37.0 37.0 35-39 36.712 37.0 37.0 37.0 37.0 37.0 40-44 36.6227 37.0 37.0 37.0 37.0 37.0 45-49 36.5086 37.0 37.0 37.0 37.0 37.0 50-54 36.5476 37.0 37.0 37.0 37.0 37.0 55-59 36.53529999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.5817 37.0 37.0 37.0 37.0 37.0 65-69 36.2749 37.0 37.0 37.0 37.0 37.0 70-74 36.486700000000006 37.0 37.0 37.0 37.0 37.0 75-79 36.538599999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.4127 37.0 37.0 37.0 37.0 37.0 85-89 36.3129 37.0 37.0 37.0 37.0 37.0 90-94 35.6182 37.0 37.0 37.0 29.8 37.0 95-99 36.2775 37.0 37.0 37.0 37.0 37.0 100-104 36.3359 37.0 37.0 37.0 37.0 37.0 105-109 36.2757 37.0 37.0 37.0 37.0 37.0 110-114 36.3315 37.0 37.0 37.0 37.0 37.0 115-119 36.632600000000004 37.0 37.0 37.0 37.0 37.0 120-124 36.6524 37.0 37.0 37.0 37.0 37.0 125-129 36.6481 37.0 37.0 37.0 37.0 37.0 130-134 36.5767 37.0 37.0 37.0 37.0 37.0 135-139 36.4963 37.0 37.0 37.0 37.0 37.0 140-144 36.3222 37.0 37.0 37.0 37.0 37.0 145-149 36.2195 37.0 37.0 37.0 37.0 37.0 150-151 34.00325 37.0 31.0 37.0 24.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 1.0 27 2.0 28 0.0 29 3.0 30 3.0 31 15.0 32 25.0 33 43.0 34 102.0 35 330.0 36 3245.0 37 231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55 8.85 4.6 42.0 2 20.70263488080301 8.080301129234629 34.57967377666248 36.637390213299874 3 20.375 11.125 24.474999999999998 44.025 4 26.424999999999997 17.05 21.55 34.975 5 26.5 25.124999999999996 22.225 26.150000000000002 6 26.474999999999998 28.15 23.375 22.0 7 19.85 23.625 36.25 20.275000000000002 8 19.8 20.125 30.599999999999998 29.475 9 20.325 21.0 31.900000000000002 26.775 10-14 22.905 24.605 24.355 28.134999999999998 15-19 24.605 22.2 24.925 28.27 20-24 25.275 23.25 24.72 26.755000000000003 25-29 23.43 22.314999999999998 25.224999999999998 29.03 30-34 23.345 21.25 24.73 30.675 35-39 23.115 22.03 25.55 29.304999999999996 40-44 23.885 21.8 25.745 28.57 45-49 23.044999999999998 21.2 26.765 28.99 50-54 22.395 22.134999999999998 26.655 28.815 55-59 22.295 23.135 25.585 28.985 60-64 22.855 21.5 26.815 28.83 65-69 22.125 21.959999999999997 26.71 29.205 70-74 23.494999999999997 23.345 24.66 28.499999999999996 75-79 24.365000000000002 23.935000000000002 23.11 28.59 80-84 23.32 22.564999999999998 24.985 29.13 85-89 23.225 22.45 25.22 29.104999999999997 90-94 22.975 22.955000000000002 25.480000000000004 28.59 95-99 20.955 23.645 25.46 29.94 100-104 22.64 22.785 25.825 28.749999999999996 105-109 23.425 23.965 24.905 27.705000000000002 110-114 23.794999999999998 23.595 25.085 27.525 115-119 24.610000000000003 22.689999999999998 24.03 28.67 120-124 24.145 23.044999999999998 25.145 27.665 125-129 23.189999999999998 23.825 22.900000000000002 30.085 130-134 25.165 22.009999999999998 24.545 28.28 135-139 24.275 22.6 25.03 28.095 140-144 24.005000000000003 24.02 22.725 29.25 145-149 23.855 23.845 24.08 28.22 150-151 22.6875 23.9125 25.7375 27.6625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 0.5 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 0.0 28 0.0 29 1.0 30 1.5 31 1.0 32 2.5 33 5.5 34 5.5 35 6.0 36 15.0 37 29.5 38 36.0 39 32.5 40 31.0 41 37.0 42 41.5 43 56.0 44 82.5 45 98.0 46 135.5 47 163.0 48 144.5 49 178.5 50 239.5 51 250.5 52 242.5 53 230.0 54 220.0 55 259.5 56 308.0 57 256.0 58 192.5 59 155.5 60 126.0 61 97.5 62 52.0 63 49.0 64 49.5 65 41.0 66 32.5 67 21.5 68 20.0 69 12.5 70 7.5 71 7.0 72 3.5 73 4.0 74 5.0 75 5.0 76 4.5 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.375 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.55 #Duplication Level Percentage of deduplicated Percentage of total 1 70.07200720072008 38.925 2 15.706570657065708 17.45 3 6.0306030603060305 10.05 4 2.25022502250225 5.0 5 1.08010801080108 3.0 6 1.3051305130513051 4.35 7 0.585058505850585 2.275 8 0.7650765076507651 3.4000000000000004 9 0.54005400540054 2.7 >10 1.6651665166516652 12.85 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 27 0.675 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 23 0.575 No Hit CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATT 21 0.525 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 20 0.5 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 19 0.475 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 19 0.475 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 18 0.44999999999999996 No Hit GGCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTC 17 0.42500000000000004 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 16 0.4 No Hit GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 16 0.4 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 15 0.375 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 15 0.375 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 15 0.375 No Hit GTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA 14 0.35000000000000003 No Hit ATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGC 13 0.325 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 13 0.325 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 13 0.325 No Hit CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC 13 0.325 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 13 0.325 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 12 0.3 No Hit CGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACT 12 0.3 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 12 0.3 No Hit CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC 11 0.27499999999999997 No Hit CCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG 11 0.27499999999999997 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 11 0.27499999999999997 No Hit GCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCC 11 0.27499999999999997 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 11 0.27499999999999997 No Hit CCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACC 11 0.27499999999999997 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 11 0.27499999999999997 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 11 0.27499999999999997 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 10 0.25 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 10 0.25 No Hit GCAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG 10 0.25 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 10 0.25 No Hit CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC 10 0.25 No Hit GTTCAGTCATAATCCGGCACACGGTAGCTTCGCGCCACTGGCTTTTCAAC 10 0.25 No Hit CCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGATCTAAA 10 0.25 No Hit CTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGG 9 0.22499999999999998 No Hit GCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGA 9 0.22499999999999998 No Hit CTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTT 9 0.22499999999999998 No Hit CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT 9 0.22499999999999998 No Hit CCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCT 9 0.22499999999999998 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 9 0.22499999999999998 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 9 0.22499999999999998 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 9 0.22499999999999998 No Hit CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT 9 0.22499999999999998 No Hit CCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAG 9 0.22499999999999998 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 9 0.22499999999999998 No Hit CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCCCGA 9 0.22499999999999998 No Hit GGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAG 8 0.2 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCC 8 0.2 No Hit GTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATC 8 0.2 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 8 0.2 No Hit CTCCTACTCATCGGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCG 8 0.2 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 8 0.2 No Hit CCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCT 8 0.2 No Hit GCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGC 8 0.2 No Hit GTCAAATTAAGCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTC 8 0.2 No Hit CGTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCC 8 0.2 No Hit GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC 8 0.2 No Hit GGCTTTACCTGATAGAACTCGTAATGGGCTCCAGCTATCCTGAGGGAAAC 8 0.2 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 8 0.2 No Hit CTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCATAAGG 8 0.2 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 8 0.2 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 8 0.2 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 8 0.2 No Hit CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAT 7 0.17500000000000002 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 7 0.17500000000000002 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 7 0.17500000000000002 No Hit GGTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGA 7 0.17500000000000002 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 7 0.17500000000000002 No Hit CCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTAT 7 0.17500000000000002 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 7 0.17500000000000002 No Hit GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG 7 0.17500000000000002 No Hit CATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAAT 7 0.17500000000000002 No Hit GTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCT 7 0.17500000000000002 No Hit CCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC 7 0.17500000000000002 No Hit CATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACC 7 0.17500000000000002 No Hit GTCGCGCGCTTTAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGT 7 0.17500000000000002 No Hit CTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTATTAATCATTACT 6 0.15 No Hit CTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTT 6 0.15 No Hit CGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTC 6 0.15 No Hit CCCCGCTTCCGACCCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTA 6 0.15 No Hit AGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGC 6 0.15 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA 6 0.15 No Hit GTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAG 6 0.15 No Hit GCCGACATTCTCGCTTCCGCTTCGTCGACCCCCGCTTTCGCGGTTGCTTC 6 0.15 No Hit GCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGG 6 0.15 No Hit CCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTA 6 0.15 No Hit CCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACA 6 0.15 No Hit GGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTT 6 0.15 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 6 0.15 No Hit GTCAGTATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGC 6 0.15 No Hit GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC 6 0.15 No Hit CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA 6 0.15 No Hit CACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCT 6 0.15 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 6 0.15 No Hit CCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT 6 0.15 No Hit CTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCATCGAAAGT 6 0.15 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 6 0.15 No Hit GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC 6 0.15 No Hit CCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAG 6 0.15 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 6 0.15 No Hit GGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCG 6 0.15 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 6 0.15 No Hit GTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTT 6 0.15 No Hit CTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGG 6 0.15 No Hit GAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAATAAA 6 0.15 No Hit CCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCA 5 0.125 No Hit CCGTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGC 5 0.125 No Hit CCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCC 5 0.125 No Hit ACTCATTCCAATTACCAGACACTAATGTGCCCGGTATTGTTATTTATTGT 5 0.125 No Hit GTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCG 5 0.125 No Hit CCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAG 5 0.125 No Hit CCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCT 5 0.125 No Hit GTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC 5 0.125 No Hit CTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGC 5 0.125 No Hit CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT 5 0.125 No Hit CTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTG 5 0.125 No Hit GTCGGGGTTTGTTGCACGTATTAGCTCTAGAATTACTACGGTTATCCGAG 5 0.125 No Hit CTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTT 5 0.125 No Hit GTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC 5 0.125 No Hit CCTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTATTAATCATTAC 5 0.125 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 5 0.125 No Hit CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 5 0.125 No Hit CCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAAACCTTGGGTTTTC 5 0.125 No Hit TGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTT 5 0.125 No Hit CCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGAA 5 0.125 No Hit GTCTATTTCACCGAGCCTCTCTCCGAGACAGTGCCCAGATCGTTACGCCT 5 0.125 No Hit CTTCAAAGTAACGATGCCGGAGGCACGACCCGGCCAATTAAGGCTAGGAG 5 0.125 No Hit GGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCC 5 0.125 No Hit CCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0125 0.0 0.0 0.0 0.0 80-81 0.07500000000000001 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 90-91 0.3 0.0 0.0 0.0 0.0 92-93 0.3375 0.0 0.0 0.0 0.0 94-95 0.48750000000000004 0.0 0.0 0.0 0.0 96-97 0.575 0.0 0.0 0.0 0.0 98-99 0.6125 0.0 0.0 0.0 0.0 100-101 0.675 0.0 0.0 0.0 0.0 102-103 0.7625 0.0 0.0 0.0 0.0 104-105 0.875 0.0 0.0 0.0 0.0 106-107 0.95 0.0 0.0 0.0 0.0 108-109 1.1375000000000002 0.0 0.0 0.0 0.0 110-111 1.45 0.0 0.0 0.0 0.0 112-113 1.65 0.0 0.0 0.0 0.0 114-115 1.75 0.0 0.0 0.0 0.0 116-117 1.9375 0.0 0.0 0.0 0.0 118-119 2.1625 0.0 0.0 0.0 0.0 120-121 2.4375 0.0 0.0 0.0 0.0 122-123 2.6125 0.0 0.0 0.0 0.0 124-125 2.95 0.0 0.0 0.0 0.0 126-127 3.2125 0.0 0.0 0.0 0.0 128-129 3.525 0.0 0.0 0.0 0.0 130-131 3.825 0.0 0.0 0.0 0.0 132-133 4.0625 0.0 0.0 0.0 0.0 134-135 4.550000000000001 0.0 0.0 0.0 0.0 136-137 4.8875 0.0 0.0 0.0 0.0 138-139 5.1625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACT 10 0.006830828 145.0 145 GAAGTTA 15 1.1411342E-4 145.0 4 TACGGAT 10 0.006830828 145.0 9 CACCGCT 10 0.006830828 145.0 8 CACCGAG 10 0.006830828 145.0 9 CGAAGTT 15 1.1411342E-4 145.0 3 TCTATTT 10 0.006830828 145.0 2 TATTTCA 10 0.006830828 145.0 4 GTCTATT 10 0.006830828 145.0 1 TCACCGA 10 0.006830828 145.0 8 GCTCCAC 10 0.006830828 145.0 4 TTACGGA 10 0.006830828 145.0 8 AAGTTAC 15 1.1411342E-4 145.0 5 CTATTTC 10 0.006830828 145.0 3 AGTTACG 15 1.1411342E-4 145.0 6 CCGAAGT 15 1.1411342E-4 145.0 2 GTTACGG 25 8.7132835E-4 87.0 7 CCCGAAG 30 0.0017973486 72.5 1 >>END_MODULE SRR18694423 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR18694423_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.0345 37.0 37.0 37.0 25.0 37.0 2 35.1935 37.0 37.0 37.0 25.0 37.0 3 35.2385 37.0 37.0 37.0 25.0 37.0 4 35.24 37.0 37.0 37.0 25.0 37.0 5 35.041 37.0 37.0 37.0 25.0 37.0 6 35.1605 37.0 37.0 37.0 25.0 37.0 7 35.0745 37.0 37.0 37.0 25.0 37.0 8 35.3745 37.0 37.0 37.0 37.0 37.0 9 35.57 37.0 37.0 37.0 37.0 37.0 10-14 35.513999999999996 37.0 37.0 37.0 37.0 37.0 15-19 35.5756 37.0 37.0 37.0 37.0 37.0 20-24 35.3651 37.0 37.0 37.0 32.2 37.0 25-29 35.5482 37.0 37.0 37.0 32.2 37.0 30-34 35.7861 37.0 37.0 37.0 34.6 37.0 35-39 35.6606 37.0 37.0 37.0 37.0 37.0 40-44 35.289300000000004 37.0 37.0 37.0 29.8 37.0 45-49 35.5154 37.0 37.0 37.0 34.6 37.0 50-54 34.873400000000004 37.0 34.6 37.0 29.4 37.0 55-59 34.2028 37.0 37.0 37.0 25.0 37.0 60-64 34.9388 37.0 37.0 37.0 27.4 37.0 65-69 34.1383 37.0 34.6 37.0 27.4 37.0 70-74 32.730399999999996 37.0 29.8 37.0 19.4 37.0 75-79 33.5682 37.0 34.6 37.0 22.2 37.0 80-84 34.6371 37.0 37.0 37.0 25.0 37.0 85-89 32.788 37.0 32.2 37.0 19.4 37.0 90-94 34.1785 37.0 37.0 37.0 25.0 37.0 95-99 34.063900000000004 37.0 37.0 37.0 25.0 37.0 100-104 33.469899999999996 37.0 37.0 37.0 25.0 37.0 105-109 34.340999999999994 37.0 37.0 37.0 25.0 37.0 110-114 34.709500000000006 37.0 37.0 37.0 25.0 37.0 115-119 34.437 37.0 37.0 37.0 25.0 37.0 120-124 34.337 37.0 37.0 37.0 25.0 37.0 125-129 34.2575 37.0 37.0 37.0 25.0 37.0 130-134 33.9435 37.0 37.0 37.0 25.0 37.0 135-139 32.221199999999996 37.0 27.4 37.0 13.8 37.0 140-144 31.652200000000004 37.0 25.0 37.0 11.0 37.0 145-149 31.197700000000005 37.0 25.0 37.0 13.8 37.0 150-151 31.26725 37.0 25.0 37.0 18.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 1.0 16 2.0 17 2.0 18 6.0 19 2.0 20 2.0 21 5.0 22 5.0 23 7.0 24 3.0 25 1.0 26 2.0 27 8.0 28 17.0 29 29.0 30 71.0 31 130.0 32 281.0 33 608.0 34 1231.0 35 1425.0 36 160.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.4 20.25 7.2749999999999995 28.075 2 30.775000000000002 21.099999999999998 27.500000000000004 20.625 3 25.1 20.674999999999997 30.375000000000004 23.849999999999998 4 30.125 30.65 19.775000000000002 19.45 5 31.374999999999996 30.0 19.1 19.525000000000002 6 26.875 31.1 18.25 23.775 7 24.175 23.025000000000002 29.875 22.925 8 28.349999999999998 20.825 23.7 27.125 9 27.900000000000002 21.025 27.474999999999998 23.599999999999998 10-14 28.615000000000002 25.97 22.21 23.205000000000002 15-19 28.249999999999996 25.480000000000004 22.975 23.294999999999998 20-24 29.48 24.97 22.525000000000002 23.025000000000002 25-29 28.749999999999996 26.57 22.2 22.48 30-34 28.21 26.43 23.165 22.195 35-39 28.71 27.084999999999997 20.665 23.54 40-44 29.09 25.245 22.564999999999998 23.1 45-49 29.060000000000002 24.815 22.425 23.7 50-54 26.700000000000003 26.07 24.425 22.805 55-59 27.134999999999998 26.41 23.59 22.865 60-64 29.955 23.605 24.23 22.21 65-69 30.125 24.77 22.865 22.24 70-74 29.765000000000004 25.025 22.705000000000002 22.505 75-79 30.11 22.42 23.115 24.355 80-84 29.03 24.805 22.795 23.369999999999997 85-89 25.75 26.57 23.630000000000003 24.05 90-94 30.185000000000002 24.89 22.41 22.515 95-99 28.705000000000002 25.485000000000003 23.305 22.505 100-104 28.32 26.029999999999998 24.085 21.565 105-109 27.935 24.240000000000002 26.52 21.305 110-114 28.64 25.285000000000004 23.69 22.384999999999998 115-119 29.080000000000002 25.34 22.75 22.830000000000002 120-124 29.12 24.795 22.39 23.695 125-129 28.62 26.33 21.445 23.605 130-134 28.655 26.279999999999998 21.3 23.765 135-139 28.144999999999996 25.145 22.915 23.794999999999998 140-144 29.435 26.07 22.005 22.49 145-149 28.050000000000004 26.355 21.72 23.875 150-151 28.499999999999996 27.3 22.55 21.65 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 1.0 25 1.0 26 0.0 27 0.5 28 1.5 29 2.5 30 3.0 31 1.5 32 1.5 33 6.5 34 14.0 35 29.5 36 33.0 37 41.5 38 59.0 39 92.0 40 89.0 41 58.5 42 68.5 43 92.5 44 109.5 45 101.5 46 113.0 47 154.0 48 171.0 49 176.5 50 165.0 51 162.0 52 209.5 53 231.0 54 234.5 55 241.0 56 208.0 57 164.0 58 141.5 59 132.0 60 111.5 61 80.0 62 60.5 63 48.5 64 46.5 65 50.0 66 47.5 67 55.5 68 60.0 69 43.5 70 25.5 71 15.5 72 9.0 73 4.0 74 5.5 75 7.0 76 6.0 77 3.0 78 2.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.8 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13915857605178 45.2 2 16.181229773462782 20.0 3 4.611650485436893 8.55 4 2.467637540453074 6.1 5 1.0922330097087378 3.375 6 0.7281553398058253 2.7 7 0.5663430420711975 2.45 8 0.28317152103559873 1.4000000000000001 9 0.24271844660194172 1.35 >10 0.6472491909385114 7.049999999999999 >50 0.04045307443365696 1.825 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 73 1.825 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 47 1.175 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 29 0.7250000000000001 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 28 0.7000000000000001 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 20 0.5 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 18 0.44999999999999996 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 17 0.42500000000000004 No Hit GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC 13 0.325 No Hit AACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTC 13 0.325 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 13 0.325 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 13 0.325 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 12 0.3 No Hit AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGA 12 0.3 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 12 0.3 No Hit ACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCT 12 0.3 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 12 0.3 No Hit GTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAG 11 0.27499999999999997 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 9 0.22499999999999998 No Hit ATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAATAGGGACAGTCGGGG 9 0.22499999999999998 No Hit AGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAA 9 0.22499999999999998 No Hit GCCATGCATGTGCAAGTATGAACTAATTTGAACTGTGAAACTGCGAATGG 9 0.22499999999999998 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 9 0.22499999999999998 No Hit AAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCA 9 0.22499999999999998 No Hit CGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCC 8 0.2 No Hit GTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAATAGGGACAGT 8 0.2 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 8 0.2 No Hit GTAATGATTAATAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGG 8 0.2 No Hit CATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAAT 8 0.2 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 8 0.2 No Hit AAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCG 8 0.2 No Hit GGCTCATTAAATCAGTTATAGTTTGTTTGATGGTACGTGCTACTCGGATA 7 0.17500000000000002 No Hit AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCA 7 0.17500000000000002 No Hit GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG 7 0.17500000000000002 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 7 0.17500000000000002 No Hit GCCGGCGATGCGCTCCTAGCCTTAATTGGCCGGGTCGTGCCTCCGGCATC 7 0.17500000000000002 No Hit GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTA 7 0.17500000000000002 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 7 0.17500000000000002 No Hit GTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCA 7 0.17500000000000002 No Hit GACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTG 7 0.17500000000000002 No Hit GTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCC 7 0.17500000000000002 No Hit GTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTG 7 0.17500000000000002 No Hit CTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTTGAACTGTGA 7 0.17500000000000002 No Hit GTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACG 7 0.17500000000000002 No Hit TCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCA 7 0.17500000000000002 No Hit AGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGT 6 0.15 No Hit GGGAAAGTGATCTCTGACCGCGTGCCTGTTGAAGAATGAGCCGGCGACTC 6 0.15 No Hit GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA 6 0.15 No Hit CAAAAGGGTAAAAGCTCGTTTGATTCTGATTTCCAGTACGAATACGAACC 6 0.15 No Hit CAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGT 6 0.15 No Hit GGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCT 6 0.15 No Hit CTTGAGAGGTGTAGGATAAGTGGGAGCCTTTACGGGCGCAAGTGAAATAC 6 0.15 No Hit CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTTGAAC 6 0.15 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 6 0.15 No Hit GTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCA 6 0.15 No Hit GGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCT 6 0.15 No Hit GAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTA 6 0.15 No Hit GTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 6 0.15 No Hit GTTGGCCTTCGGGATCGGAGTAATGATTAATAGGGACAGTCGGGGGCATT 6 0.15 No Hit CAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 6 0.15 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 6 0.15 No Hit GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCATCGCTCTGGATAC 6 0.15 No Hit GTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAG 6 0.15 No Hit GCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAGCGTGGC 5 0.125 No Hit GTTGTGGTTAGGGGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTC 5 0.125 No Hit TTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTG 5 0.125 No Hit CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGG 5 0.125 No Hit GGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit AAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTT 5 0.125 No Hit GGGGCGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCCCC 5 0.125 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 5 0.125 No Hit TGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGC 5 0.125 No Hit GTTGGAGCACGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCG 5 0.125 No Hit GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTA 5 0.125 No Hit CAGAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGATTCTGATTT 5 0.125 No Hit GGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCC 5 0.125 No Hit GTCCCCTCCTTTTGGCTCCAAGGCCCGGTCTGACCGGGCCGATCCGGGCG 5 0.125 No Hit CGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCA 5 0.125 No Hit GGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGG 5 0.125 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 5 0.125 No Hit GACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAAC 5 0.125 No Hit GTCGGAAGCGGGGCAAGTCCCCTCCTTTTGGCTCCAAGGCCCGGTCTGAC 5 0.125 No Hit GTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATG 5 0.125 No Hit TGTGCAAGTATGAACTAATTTGAACTGTGAAACTGCGAATGGCTCATTAA 5 0.125 No Hit CGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACA 5 0.125 No Hit AGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGTGCTGTGCG 5 0.125 No Hit GCTCATTAAATCAGTTATAGTTTGTTTGATGGTACGTGCTACTCGGATAA 5 0.125 No Hit CTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACC 5 0.125 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0125 0.0 0.0 0.0 0.0 80-81 0.0625 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.2375 0.0 0.0 0.0 0.0 90-91 0.275 0.0 0.0 0.0 0.0 92-93 0.3125 0.0 0.0 0.0 0.0 94-95 0.4625 0.0 0.0 0.0 0.0 96-97 0.55 0.0 0.0 0.0 0.0 98-99 0.5875 0.0 0.0 0.0 0.0 100-101 0.65 0.0 0.0 0.0 0.0 102-103 0.7375 0.0 0.0 0.0 0.0 104-105 0.85 0.0 0.0 0.0 0.0 106-107 0.925 0.0 0.0 0.0 0.0 108-109 1.1124999999999998 0.0 0.0 0.0 0.0 110-111 1.425 0.0 0.0 0.0 0.0 112-113 1.625 0.0 0.0 0.0 0.0 114-115 1.725 0.0 0.0 0.0 0.0 116-117 1.9125 0.0 0.0 0.0 0.0 118-119 2.1375 0.0 0.0 0.0 0.0 120-121 2.4125 0.0 0.0 0.0 0.0 122-123 2.5875 0.0 0.0 0.0 0.0 124-125 2.925 0.0 0.0 0.0 0.0 126-127 3.1875 0.0 0.0 0.0 0.0 128-129 3.5 0.0 0.0 0.0 0.0 130-131 3.8 0.0 0.0 0.0 0.0 132-133 4.025 0.0 0.0 0.0 0.0 134-135 4.5 0.0 0.0 0.0 0.0 136-137 4.8375 0.0 0.0 0.0 0.0 138-139 5.112500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGACCA 10 0.006830828 145.0 1 CCAGGCC 10 0.006830828 145.0 5 >>END_MODULE Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651013 spots for SRR18694423.sra Written 651013 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra Read 651005 spots for SRR18694423.sra Written 651005 spots for SRR18694423.sra SRR ids: ['SRR18694423.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_h4jozsd5 SRR18694423.sra spots: 13020108 blocks: [[1, 651005], [651006, 1302010], [1302011, 1953015], [1953016, 2604020], [2604021, 3255025], [3255026, 3906030], [3906031, 4557035], [4557036, 5208040], [5208041, 5859045], [5859046, 6510050], [6510051, 7161055], [7161056, 7812060], [7812061, 8463065], [8463066, 9114070], [9114071, 9765075], [9765076, 10416080], [10416081, 11067085], [11067086, 11718090], [11718091, 12369095], [12369096, 13020108]] SRR18694423 file size 4403101 SRR18694423 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR18694423 SRR18694423_1.fastq SRR18694423_2.fastq Input file: SRR18694423_1.fastq Paired file: SRR18694423_2.fastq trimmed: SRR18694423-trimmed-pair1.fastq, SRR18694423-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 06:31:18 2024 >> started Tue Dec 10 06:31:32 2024 >> done (14.325s) 13020108 read pairs processed; of these: 77 ( 0.00%) short read pairs filtered out after trimming by size control 5623 ( 0.04%) empty read pairs filtered out after trimming by size control 13014408 (99.96%) read pairs available; of these: 1012832 ( 7.78%) trimmed read pairs available after processing 12001576 (92.22%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 7 0.00% 20 12 0.00% 21 4 0.00% 22 8 0.00% 23 11 0.00% 24 13 0.00% 25 18 0.00% 26 7 0.00% 27 12 0.00% 28 10 0.00% 29 9 0.00% 30 22 0.00% 31 21 0.00% 32 21 0.00% 33 23 0.00% 34 22 0.00% 35 28 0.00% 36 20 0.00% 37 26 0.00% 38 20 0.00% 39 22 0.00% 40 29 0.00% 41 27 0.00% 42 16 0.00% 43 38 0.00% 44 34 0.00% 45 33 0.00% 46 40 0.00% 47 48 0.00% 48 36 0.00% 49 50 0.00% 50 57 0.00% 51 40 0.00% 52 70 0.00% 53 70 0.00% 54 71 0.00% 55 79 0.00% 56 76 0.00% 57 81 0.00% 58 136 0.00% 59 135 0.00% 60 120 0.00% 61 176 0.00% 62 189 0.00% 63 172 0.00% 64 203 0.00% 65 234 0.00% 66 265 0.00% 67 250 0.00% 68 370 0.00% 69 397 0.00% 70 370 0.00% 71 430 0.00% 72 574 0.00% 73 654 0.01% 74 647 0.00% 75 649 0.00% 76 801 0.01% 77 884 0.01% 78 945 0.01% 79 1179 0.01% 80 1170 0.01% 81 1395 0.01% 82 1595 0.01% 83 1724 0.01% 84 1803 0.01% 85 2188 0.02% 86 2910 0.02% 87 2748 0.02% 88 2893 0.02% 89 2735 0.02% 90 2920 0.02% 91 3257 0.03% 92 3526 0.03% 93 4077 0.03% 94 4017 0.03% 95 4556 0.04% 96 5058 0.04% 97 5062 0.04% 98 5516 0.04% 99 5733 0.04% 100 5992 0.05% 101 6244 0.05% 102 6160 0.05% 103 6591 0.05% 104 7183 0.06% 105 6704 0.05% 106 7016 0.05% 107 7629 0.06% 108 7766 0.06% 109 8498 0.07% 110 8482 0.07% 111 9192 0.07% 112 10063 0.08% 113 9315 0.07% 114 10431 0.08% 115 11562 0.09% 116 12121 0.09% 117 11977 0.09% 118 11260 0.09% 119 13328 0.10% 120 16581 0.13% 121 13965 0.11% 122 15346 0.12% 123 20410 0.16% 124 18785 0.14% 125 18918 0.15% 126 18888 0.15% 127 18130 0.14% 128 18597 0.14% 129 20586 0.16% 130 20033 0.15% 131 20515 0.16% 132 23248 0.18% 133 22527 0.17% 134 21464 0.16% 135 22933 0.18% 136 25052 0.19% 137 25656 0.20% 138 24502 0.19% 139 25269 0.19% 140 26701 0.21% 141 26834 0.21% 142 29252 0.22% 143 29873 0.23% 144 33009 0.25% 145 30956 0.24% 146 31211 0.24% 147 34687 0.27% 148 33508 0.26% 149 36999 0.28% 150 34978 0.27% 151 12001576 92.22% 13014408 reads passed initial QC criterion=sequence-density sequence-density=4.25 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=28 prefix-density=3.86 prefix-fanout=2.0 sequence=CCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGCGATGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCACGACCCGGCCAATTAAGGCTAGGAGCGCATCGCCGGCTGAAGGGTCGAGTAGGTCGGTGCTCGCCGTGAGGCGGACCGGCCGACCCGGCCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=812.59 fanout-score-rank=1 prefix-density=6.33 prefix-fanout=1.0 sequence=TGCAATGATCTGTCCCCATCACG criterion=sequence-density sequence-density=1.65 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=33 prefix-density=1.67 prefix-fanout=2.0 sequence=CAAGGCTAAATAC criterion=fanout-score sequence-density=0.11 sequence-density-rank=30 fanout-score=65.79 fanout-score-rank=1 prefix-density=7.37 prefix-fanout=1.0 sequence=CTAGCTATGCGCAGCCATCCCTC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x CCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGCGATGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCACGACCCGGCCAATTAAGGCTAGGAGCGCATCGCCGGCTGAAGGGTCGAGTAGGTCGGTGCTCGCCGTGAGGCGGACCGGCCGACCCGGCCC -y CAAGGCTAAATAC -o SRR18694423 SRR18694423_1.fastq SRR18694423_2.fastq Input file: SRR18694423_1.fastq Paired file: SRR18694423_2.fastq trimmed: SRR18694423-trimmed-pair1.fastq, SRR18694423-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): CCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATT -- paired 3' end adapter sequence (-y): CAAGGCTAAATAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 06:33:12 2024 >> started Tue Dec 10 06:33:20 2024 >> done (7.475s) 6507204 read pairs processed; of these: 1797 ( 0.03%) short read pairs filtered out after trimming by size control 43366 ( 0.67%) empty read pairs filtered out after trimming by size control 6462041 (99.31%) read pairs available; of these: 6 ( 0.00%) trimmed read pairs available after processing 6462035 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 4 0.00% 20 5 0.00% 21 1 0.00% 22 3 0.00% 23 8 0.00% 24 8 0.00% 25 5 0.00% 26 6 0.00% 27 10 0.00% 28 4 0.00% 29 4 0.00% 30 11 0.00% 31 5 0.00% 32 13 0.00% 33 9 0.00% 34 12 0.00% 35 16 0.00% 36 7 0.00% 37 8 0.00% 38 13 0.00% 39 15 0.00% 40 14 0.00% 41 13 0.00% 42 9 0.00% 43 21 0.00% 44 17 0.00% 45 15 0.00% 46 23 0.00% 47 21 0.00% 48 13 0.00% 49 27 0.00% 50 26 0.00% 51 20 0.00% 52 41 0.00% 53 41 0.00% 54 44 0.00% 55 39 0.00% 56 34 0.00% 57 38 0.00% 58 70 0.00% 59 66 0.00% 60 66 0.00% 61 73 0.00% 62 94 0.00% 63 98 0.00% 64 97 0.00% 65 126 0.00% 66 124 0.00% 67 117 0.00% 68 180 0.00% 69 210 0.00% 70 180 0.00% 71 218 0.00% 72 279 0.00% 73 322 0.00% 74 352 0.01% 75 330 0.01% 76 400 0.01% 77 501 0.01% 78 472 0.01% 79 618 0.01% 80 589 0.01% 81 699 0.01% 82 775 0.01% 83 862 0.01% 84 895 0.01% 85 1107 0.02% 86 1451 0.02% 87 1333 0.02% 88 1417 0.02% 89 1382 0.02% 90 1456 0.02% 91 1627 0.03% 92 1770 0.03% 93 2012 0.03% 94 2008 0.03% 95 2294 0.04% 96 2490 0.04% 97 2542 0.04% 98 2784 0.04% 99 2850 0.04% 100 3029 0.05% 101 3126 0.05% 102 3074 0.05% 103 3265 0.05% 104 3578 0.06% 105 3398 0.05% 106 3525 0.05% 107 3874 0.06% 108 3865 0.06% 109 4136 0.06% 110 4099 0.06% 111 4589 0.07% 112 4918 0.08% 113 4590 0.07% 114 5238 0.08% 115 5863 0.09% 116 6012 0.09% 117 6046 0.09% 118 5706 0.09% 119 6618 0.10% 120 8091 0.13% 121 7001 0.11% 122 7564 0.12% 123 10215 0.16% 124 9405 0.15% 125 9374 0.15% 126 9453 0.15% 127 8905 0.14% 128 9167 0.14% 129 10194 0.16% 130 9994 0.15% 131 10010 0.15% 132 11686 0.18% 133 11058 0.17% 134 10643 0.16% 135 11383 0.18% 136 12560 0.19% 137 12932 0.20% 138 12050 0.19% 139 12684 0.20% 140 13408 0.21% 141 13243 0.20% 142 14707 0.23% 143 14833 0.23% 144 16661 0.26% 145 15264 0.24% 146 15417 0.24% 147 17296 0.27% 148 16743 0.26% 149 18326 0.28% 150 17705 0.27% 151 5957559 92.19% criterion=sequence-density sequence-density=2.83 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=28 prefix-density=2.81 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=742.02 fanout-score-rank=1 prefix-density=6.37 prefix-fanout=1.0 sequence=TGCAATGATCTGTCCCCATCACG criterion=sequence-density sequence-density=1.64 sequence-density-rank=1 fanout-score=6.65 fanout-score-rank=14 prefix-density=6.34 prefix-fanout=1.7 sequence=TGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCAGGAATCGCTAGTAATCGCCGGTCAGCCATACGGCGGTGAATCCGTTCCCGGGCCTTGTACACACC criterion=fanout-score sequence-density=0.16 sequence-density-rank=21 fanout-score=100.73 fanout-score-rank=1 prefix-density=7.98 prefix-fanout=2.0 sequence=AACTGCGAATGACTCATTAAATC SRR18694423 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 06:34:13 Started mapping on | Dec 10 06:34:14 Finished on | Dec 10 06:35:28 Mapping speed, Million of reads per hour | 630.94 Number of input reads | 12969245 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 3425526 Uniquely mapped reads % | 26.41% Average mapped length | 298.56 Number of splices: Total | 1684486 Number of splices: Annotated (sjdb) | 1578889 Number of splices: GT/AG | 1659151 Number of splices: GC/AG | 18463 Number of splices: AT/AC | 1032 Number of splices: Non-canonical | 5840 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.01% Deletion average length | 2.15 Insertion rate per base | 0.00% Insertion average length | 2.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1722724 % of reads mapped to multiple loci | 13.28% Number of reads mapped to too many loci | 628062 % of reads mapped to too many loci | 4.84% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.97% % of reads unmapped: other | 53.49% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 7820995 7820995 7820995 N_multimapping 1722724 1722724 1722724 N_noFeature 1792172 3370982 1807937 N_ambiguous 62384 393 23974 UnstrandedReadsAssigned:1570970 PositiveStrandReadsAssigned:54151 NegativeStrandReadsAssigned:1593615 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR18694423 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR18694423-trimmed-pair1.fastq SRR18694423-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 12,969,245 reads, 2,257,164 reads pseudoaligned [quant] estimated average fragment length: 252.396 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,048 rounds 52973 SRR18694423.ke.tsv 35125 SRR18694423.se.tsv 88098 total ==> SRR18694423.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 685.021 14.2509 5.58231 PNS24247 1044 792.604 2.4558 0.831403 PNS24249 1928 1676.6 3.38167 0.541222 PNS24246 1044 792.604 2.4558 0.831403 PNS24248 1044 792.604 2.4558 0.831403 PNS24244 1471 1219.6 0 0 PNS24243 293 91.2876 0 0 KQK14069 1603 1351.6 402.634 79.9348 KQK14071 474 238.959 3.80168 4.269 ==> SRR18694423.se.tsv <== BRADI_1g14170v3 435 BRADI_1g53295v3 0 BRADI_1g59795v3 45 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 210 BRADI_1g74790v3 9 BRADI_1g09890v3 2 BRADI_1g77505v3 35 BRADI_1g48960v3 0 SRR18694423 completed mapping pipeline successfully