Starting /dee2/code/volunteer_pipeline.sh SRR21853440 current disk space = 1550519586816 free memory = 1598584272 SRR21853440 SRAfilesize df2447331e2a121219dc64c94e5f46cc SRR21853440.sra SRR21853440.sra file validated SRR21853440 is single end SRR21853440 is conventional basespace SRR21853440 read1 length is 64-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR21853440_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 64-101 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.252 37.0 37.0 37.0 25.0 37.0 2 35.394 37.0 37.0 37.0 37.0 37.0 3 35.72475 37.0 37.0 37.0 37.0 37.0 4 35.8105 37.0 37.0 37.0 37.0 37.0 5 35.9965 37.0 37.0 37.0 37.0 37.0 6 35.825 37.0 37.0 37.0 37.0 37.0 7 35.745 37.0 37.0 37.0 37.0 37.0 8 35.8395 37.0 37.0 37.0 37.0 37.0 9 35.9055 37.0 37.0 37.0 37.0 37.0 10-11 36.0215 37.0 37.0 37.0 37.0 37.0 12-13 35.879000000000005 37.0 37.0 37.0 37.0 37.0 14-15 35.90875 37.0 37.0 37.0 37.0 37.0 16-17 35.888000000000005 37.0 37.0 37.0 37.0 37.0 18-19 35.795500000000004 37.0 37.0 37.0 37.0 37.0 20-21 35.83775 37.0 37.0 37.0 37.0 37.0 22-23 35.857749999999996 37.0 37.0 37.0 37.0 37.0 24-25 35.7725 37.0 37.0 37.0 37.0 37.0 26-27 35.67274999999999 37.0 37.0 37.0 37.0 37.0 28-29 35.6545 37.0 37.0 37.0 37.0 37.0 30-31 35.587500000000006 37.0 37.0 37.0 37.0 37.0 32-33 35.648250000000004 37.0 37.0 37.0 37.0 37.0 34-35 35.6365 37.0 37.0 37.0 37.0 37.0 36-37 35.570750000000004 37.0 37.0 37.0 37.0 37.0 38-39 35.5865 37.0 37.0 37.0 37.0 37.0 40-41 35.5125 37.0 37.0 37.0 37.0 37.0 42-43 35.54925 37.0 37.0 37.0 37.0 37.0 44-45 35.45075 37.0 37.0 37.0 37.0 37.0 46-47 35.586 37.0 37.0 37.0 37.0 37.0 48-49 35.46225 37.0 37.0 37.0 37.0 37.0 50-51 35.4985 37.0 37.0 37.0 37.0 37.0 52-53 35.41275 37.0 37.0 37.0 37.0 37.0 54-55 35.4975 37.0 37.0 37.0 37.0 37.0 56-57 35.36875 37.0 37.0 37.0 37.0 37.0 58-59 35.40625 37.0 37.0 37.0 37.0 37.0 60-61 35.34975 37.0 37.0 37.0 37.0 37.0 62-63 35.449 37.0 37.0 37.0 37.0 37.0 64-65 35.39629513628407 37.0 37.0 37.0 37.0 37.0 66-67 35.412603150787696 37.0 37.0 37.0 31.0 37.0 68-69 35.353088272068014 37.0 37.0 37.0 37.0 37.0 70-71 35.32208052013003 37.0 37.0 37.0 37.0 37.0 72-73 35.23605901475369 37.0 37.0 37.0 31.0 37.0 74-75 35.192798199549884 37.0 37.0 37.0 25.0 37.0 76-77 35.0977744436109 37.0 37.0 37.0 25.0 37.0 78-79 35.207051762940736 37.0 37.0 37.0 25.0 37.0 80-81 35.249562390597646 37.0 37.0 37.0 31.0 37.0 82-83 35.25912956478239 37.0 37.0 37.0 31.0 37.0 84-85 35.1648324162081 37.0 37.0 37.0 25.0 37.0 86-87 35.188391293470104 37.0 37.0 37.0 25.0 37.0 88-89 35.01851388541406 37.0 37.0 37.0 25.0 37.0 90-91 34.99274455841881 37.0 37.0 37.0 25.0 37.0 92-93 35.03603891751576 37.0 37.0 37.0 25.0 37.0 94-95 35.09787234042553 37.0 37.0 37.0 25.0 37.0 96-97 35.04712257480925 37.0 37.0 37.0 25.0 37.0 98-99 34.835064499624465 37.0 37.0 37.0 25.0 37.0 100-101 34.91157829774744 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2101 1 0.0 2101 2 0.0 2101 3 0.0 2101 4 0.0 2101 5 0.0 2101 6 0.0 2101 7 0.0 2101 8 0.0 2101 9 0.0 2101 10-11 0.0 2101 12-13 0.0 2101 14-15 0.0 2101 16-17 0.0 2101 18-19 0.0 2101 20-21 0.0 2101 22-23 0.0 2101 24-25 0.0 2101 26-27 0.0 2101 28-29 0.0 2101 30-31 0.0 2101 32-33 0.0 2101 34-35 0.0 2101 36-37 0.0 2101 38-39 0.0 2101 40-41 0.0 2101 42-43 0.0 2101 44-45 0.0 2101 46-47 0.0 2101 48-49 0.0 2101 50-51 0.0 2101 52-53 0.0 2101 54-55 0.0 2101 56-57 0.0 2101 58-59 0.0 2101 60-61 0.0 2101 62-63 0.0 2101 64-65 0.0 2101 66-67 0.0 2101 68-69 0.0 2101 70-71 0.0 2101 72-73 0.0 2101 74-75 0.0 2101 76-77 0.0 2101 78-79 0.0 2101 80-81 0.0 2101 82-83 0.0 2101 84-85 0.0 2101 86-87 0.0 2101 88-89 0.0 2101 90-91 0.0 2101 92-93 0.0 2101 94-95 0.0 2101 96-97 0.0 2101 98-99 0.0 2101 100-101 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 4.0 24 3.0 25 5.0 26 16.0 27 25.0 28 34.0 29 67.0 30 83.0 31 97.0 32 122.0 33 176.0 34 304.0 35 641.0 36 2015.0 37 406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.840920460230116 10.48024012006003 15.78289144572286 41.89594797398699 2 27.6 20.175 27.500000000000004 24.725 3 27.806951737934483 23.10577644411103 21.48037009252313 27.60690172543136 4 29.975 29.775000000000002 17.0 23.25 5 29.15 30.15 19.25 21.45 6 22.575 30.9 21.55 24.975 7 19.575 15.225 38.925 26.275 8 23.599999999999998 19.85 23.925 32.625 9 23.45 20.424999999999997 27.05 29.075 10-11 26.8625 26.6625 18.3 28.175 12-13 25.9875 20.5 24.2875 29.225 14-15 25.624999999999996 23.1625 24.325 26.887499999999996 16-17 25.9625 24.474999999999998 22.25 27.3125 18-19 26.087500000000002 24.5 23.25 26.1625 20-21 25.924999999999997 23.6875 23.799999999999997 26.5875 22-23 26.187500000000004 23.575 24.025 26.2125 24-25 26.025 23.799999999999997 24.0 26.174999999999997 26-27 25.95 23.275000000000002 24.025 26.75 28-29 25.674999999999997 23.962500000000002 23.3125 27.05 30-31 25.624999999999996 23.3625 23.825 27.187499999999996 32-33 26.8 24.0625 22.8375 26.3 34-35 26.2875 24.2625 23.1125 26.337500000000002 36-37 26.9125 23.200000000000003 23.2375 26.650000000000002 38-39 27.125 23.4125 22.75 26.7125 40-41 26.900000000000002 23.7125 22.5125 26.875 42-43 26.025 24.3125 23.6125 26.05 44-45 25.95 23.4875 23.3375 27.224999999999998 46-47 26.25 24.25 23.025000000000002 26.474999999999998 48-49 25.5625 24.025 23.35 27.0625 50-51 25.4625 24.0375 23.0375 27.462500000000002 52-53 27.537499999999998 23.0 23.0625 26.400000000000002 54-55 26.075 24.1875 22.287499999999998 27.450000000000003 56-57 25.75 23.7375 23.150000000000002 27.3625 58-59 28.0875 23.7375 22.4625 25.7125 60-61 26.1625 24.1875 23.525 26.125 62-63 27.287499999999998 23.3875 22.287499999999998 27.037499999999998 64-65 27.140892611576444 24.16552069008626 22.802850356294538 25.890736342042754 66-67 26.756689172293076 22.593148287071767 24.093523380845213 26.556639159789945 68-69 26.65666416604151 23.34333583395849 22.1055263815954 27.894473618404604 70-71 26.894223555888974 24.031007751937985 22.255563890972745 26.819204801200303 72-73 26.056514128532132 23.0432608152038 23.13078269567392 27.769442360590148 74-75 26.481620405101275 24.081020255063766 23.118279569892472 26.319079769942487 76-77 26.294073518379594 22.680670167541887 23.3183295823956 27.70692673168292 78-79 26.6816704176044 23.893473368342086 22.693173293323333 26.731682920730183 80-81 26.494123530882717 22.768192048012004 23.0432608152038 27.694423605901473 82-83 26.575787893946973 22.823911955977987 22.373686843421712 28.226613306653327 84-85 27.063531765882942 23.274137068534266 22.198599299649825 27.463731865932967 86-87 27.47060295221416 22.52939704778584 23.417563172379285 26.582436827620715 88-89 26.8951713785339 23.667750813109834 22.254190642982234 27.18288716537403 90-91 26.46985238929197 23.8804103077308 22.12909682261696 27.52064048036027 92-93 27.593542735577525 23.488925040670754 22.68802402703041 26.229508196721312 94-95 27.334167709637047 22.40300375469337 23.216520650813514 27.04630788485607 96-97 27.862691054873466 22.037083437734903 23.60310699072914 26.49711851666249 98-99 26.53968253968254 22.133333333333333 23.56825396825397 27.75873015873016 100-101 28.486367909746164 9.934189909119398 27.279849576935128 34.29959260419931 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 1.0 23 2.0 24 1.5 25 0.5 26 1.5 27 2.5 28 1.0 29 2.5 30 4.0 31 5.0 32 5.5 33 6.0 34 11.5 35 20.0 36 24.0 37 33.5 38 43.5 39 49.0 40 62.5 41 75.0 42 91.5 43 101.5 44 103.5 45 118.0 46 133.0 47 142.5 48 147.0 49 149.5 50 155.5 51 164.5 52 160.5 53 160.0 54 166.0 55 169.0 56 181.0 57 166.0 58 148.0 59 136.0 60 113.5 61 102.0 62 92.0 63 80.5 64 78.0 65 68.5 66 53.0 67 61.0 68 62.5 69 52.0 70 46.5 71 40.0 72 37.5 73 36.0 74 31.5 75 27.5 76 29.0 77 20.0 78 8.5 79 5.0 80 6.5 81 5.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.025 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 64 1.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 1.0 82 0.0 83 0.0 84 0.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 2.0 96 4.0 97 22.0 98 59.0 99 251.0 100 932.0 101 2725.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.25 #Duplication Level Percentage of deduplicated Percentage of total 1 88.59598853868195 77.3 2 9.512893982808022 16.6 3 1.2034383954154728 3.15 4 0.3151862464183381 1.0999999999999999 5 0.20057306590257878 0.8750000000000001 6 0.14326647564469913 0.75 7 0.0 0.0 8 0.0 0.0 9 0.028653295128939826 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA 9 0.22499999999999998 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 6 0.15 No Hit GAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTT 6 0.15 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 6 0.15 No Hit CACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTT 6 0.15 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 6 0.15 No Hit CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGG 5 0.125 No Hit CAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC 5 0.125 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 5 0.125 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 5 0.125 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCC 5 0.125 No Hit CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTC 5 0.125 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0125 0.0 0.0 26-27 0.0 0.0 0.025 0.0 0.0 28-29 0.0 0.0 0.025 0.0 0.0 30-31 0.0 0.0 0.025 0.0 0.0 32-33 0.0 0.0 0.025 0.0 0.0 34-35 0.0 0.0 0.025 0.0 0.0 36-37 0.0 0.0 0.025 0.0 0.0 38-39 0.0 0.0 0.025 0.0 0.0 40-41 0.0 0.0 0.025 0.0 0.0 42-43 0.0 0.0 0.025 0.0 0.0 44-45 0.0 0.0 0.025 0.0 0.0 46-47 0.0 0.0 0.025 0.0 0.0 48-49 0.0 0.0 0.025 0.0 0.0 50-51 0.0 0.0 0.025 0.0 0.0 52-53 0.0 0.0 0.025 0.0 0.0 54-55 0.0 0.0 0.025 0.0 0.0 56-57 0.0 0.0 0.025 0.0 0.0 58-59 0.0 0.0 0.025 0.0 0.0 60-61 0.0 0.0 0.025 0.0 0.0 62-63 0.0 0.0 0.025 0.0 0.0 64-65 0.0 0.0 0.025 0.0 0.0 66-67 0.0 0.0 0.025 0.0 0.0 68-69 0.0 0.0 0.025 0.0 0.0 70-71 0.0 0.0 0.025 0.0 0.0 72-73 0.0 0.0 0.025 0.0 0.0 74-75 0.0 0.0 0.025 0.0 0.0 76-77 0.0 0.0 0.025 0.0 0.0 78-79 0.0 0.0 0.025 0.0 0.0 80-81 0.0 0.0 0.025 0.0 0.0 82-83 0.0 0.0 0.025 0.0 0.0 84-85 0.0 0.0 0.025 0.0 0.0 86-87 0.0 0.0 0.025 0.0 0.0 88-89 0.0 0.0 0.025 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128307 spots for SRR21853440.sra Written 128307 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra Read 128292 spots for SRR21853440.sra Written 128292 spots for SRR21853440.sra SRR ids: ['SRR21853440.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ab5uzurh SRR21853440.sra spots: 2565855 blocks: [[1, 128292], [128293, 256584], [256585, 384876], [384877, 513168], [513169, 641460], [641461, 769752], [769753, 898044], [898045, 1026336], [1026337, 1154628], [1154629, 1282920], [1282921, 1411212], [1411213, 1539504], [1539505, 1667796], [1667797, 1796088], [1796089, 1924380], [1924381, 2052672], [2052673, 2180964], [2180965, 2309256], [2309257, 2437548], [2437549, 2565855]] SRR21853440 file size 689090 SRR21853440 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR21853440 SRR21853440_1.fastq Input file: SRR21853440_1.fastq trimmed: SRR21853440-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Fri Dec 6 14:50:36 2024 >> started Fri Dec 6 14:50:39 2024 >> done (2.460s) 2565855 reads processed; of these: 6 ( 0.00%) short reads filtered out after trimming by size control 7938 ( 0.31%) empty reads filtered out after trimming by size control 2557911 (99.69%) reads available; of these: 171 ( 0.01%) trimmed reads available after processing 2557740 (99.99%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 1 0.00% 25 1 0.00% 26 0 0.00% 27 1 0.00% 28 0 0.00% 29 0 0.00% 30 0 0.00% 31 0 0.00% 32 1 0.00% 33 1 0.00% 34 3 0.00% 35 15 0.00% 36 18 0.00% 37 33 0.00% 38 27 0.00% 39 32 0.00% 40 33 0.00% 41 25 0.00% 42 27 0.00% 43 30 0.00% 44 30 0.00% 45 28 0.00% 46 18 0.00% 47 30 0.00% 48 27 0.00% 49 24 0.00% 50 24 0.00% 51 22 0.00% 52 27 0.00% 53 27 0.00% 54 28 0.00% 55 27 0.00% 56 20 0.00% 57 25 0.00% 58 21 0.00% 59 30 0.00% 60 31 0.00% 61 33 0.00% 62 30 0.00% 63 30 0.00% 64 31 0.00% 65 36 0.00% 66 41 0.00% 67 34 0.00% 68 37 0.00% 69 47 0.00% 70 37 0.00% 71 47 0.00% 72 34 0.00% 73 57 0.00% 74 44 0.00% 75 36 0.00% 76 40 0.00% 77 55 0.00% 78 52 0.00% 79 60 0.00% 80 53 0.00% 81 53 0.00% 82 53 0.00% 83 54 0.00% 84 60 0.00% 85 72 0.00% 86 66 0.00% 87 84 0.00% 88 97 0.00% 89 85 0.00% 90 84 0.00% 91 369 0.01% 92 167 0.01% 93 233 0.01% 94 166 0.01% 95 517 0.02% 96 2643 0.10% 97 10956 0.43% 98 38686 1.51% 99 167148 6.53% 100 553089 21.62% 101 1781707 69.65% 2557911 reads passed initial QC criterion=sequence-density sequence-density=0.72 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=26 prefix-density=0.72 prefix-fanout=2.0 sequence=TTCGCTATCGGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=13.51 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=1.4 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACA Started job on | Dec 06 14:50:55 Started mapping on | Dec 06 14:50:56 Finished on | Dec 06 14:51:02 Mapping speed, Million of reads per hour | 1534.75 Number of input reads | 2557911 Average input read length | 100 UNIQUE READS: Uniquely mapped reads number | 1292685 Uniquely mapped reads % | 50.54% Average mapped length | 100.26 Number of splices: Total | 410808 Number of splices: Annotated (sjdb) | 390181 Number of splices: GT/AG | 404474 Number of splices: GC/AG | 5201 Number of splices: AT/AC | 237 Number of splices: Non-canonical | 896 Mismatch rate per base, % | 0.19% Deletion rate per base | 0.00% Deletion average length | 1.71 Insertion rate per base | 0.00% Insertion average length | 1.54 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 508447 % of reads mapped to multiple loci | 19.88% Number of reads mapped to too many loci | 662588 % of reads mapped to too many loci | 25.90% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.68% % of reads unmapped: other | 2.00% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 756779 756779 756779 N_multimapping 508447 508447 508447 N_noFeature 97647 689266 684949 N_ambiguous 19260 1787 1497 UnstrandedReadsAssigned:1175778 PositiveStrandReadsAssigned:601632 NegativeStrandReadsAssigned:606239 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=100 echo kmer=95 SRR21853440 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR21853440-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 2,557,911 reads, 1,401,805 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,005 rounds 52973 SRR21853440.ke.tsv 35125 SRR21853440.se.tsv 88098 total ==> SRR21853440.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 4.34375 5.10635 PNS24247 1044 945 2.91667 3.03687 PNS24249 1928 1829 12.9062 6.94317 PNS24246 1044 945 2.91667 3.03687 PNS24248 1044 945 2.91667 3.03687 PNS24244 1471 1372 0 0 PNS24243 293 194 0 0 KQK14069 1603 1504 97 63.4593 KQK14071 474 375 0 0 ==> SRR21853440.se.tsv <== BRADI_1g14170v3 97 BRADI_1g53295v3 10 BRADI_1g59795v3 13 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 142 BRADI_1g74790v3 19 BRADI_1g09890v3 0 BRADI_1g77505v3 2 BRADI_1g48960v3 0 SRR21853440 completed mapping pipeline successfully