Starting /dee2/code/volunteer_pipeline.sh SRR21853441 current disk space = 1550501572608 free memory = 1599940204 SRR21853441 SRAfilesize e785e8400bb2da0009fa13603b483e16 SRR21853441.sra SRR21853441.sra file validated SRR21853441 is single end SRR21853441 is conventional basespace SRR21853441 read1 length is 35-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR21853441_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-101 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.373 32.0 32.0 32.0 32.0 32.0 2 31.4 32.0 32.0 32.0 32.0 32.0 3 31.547 32.0 32.0 32.0 32.0 32.0 4 31.60675 32.0 32.0 32.0 32.0 32.0 5 31.6135 32.0 32.0 32.0 32.0 32.0 6 34.74525 36.0 36.0 36.0 36.0 36.0 7 35.18125 36.0 36.0 36.0 36.0 36.0 8 35.01375 36.0 36.0 36.0 36.0 36.0 9 35.0285 36.0 36.0 36.0 36.0 36.0 10-11 35.149625 36.0 36.0 36.0 36.0 36.0 12-13 35.11525 36.0 36.0 36.0 36.0 36.0 14-15 35.06525 36.0 36.0 36.0 36.0 36.0 16-17 35.019125 36.0 36.0 36.0 36.0 36.0 18-19 35.102000000000004 36.0 36.0 36.0 36.0 36.0 20-21 35.043125 36.0 36.0 36.0 36.0 36.0 22-23 35.004875 36.0 36.0 36.0 36.0 36.0 24-25 34.979124999999996 36.0 36.0 36.0 36.0 36.0 26-27 34.942 36.0 36.0 36.0 36.0 36.0 28-29 34.96925 36.0 36.0 36.0 34.0 36.0 30-31 34.954875 36.0 36.0 36.0 34.0 36.0 32-33 34.803625 36.0 36.0 36.0 32.0 36.0 34-35 34.81075 36.0 36.0 36.0 34.0 36.0 36-37 34.72005501375344 36.0 36.0 36.0 32.0 36.0 38-39 34.57458786907833 36.0 36.0 36.0 32.0 36.0 40-41 34.67271135567784 36.0 36.0 36.0 34.0 36.0 42-43 34.75260065609487 36.0 36.0 36.0 32.0 36.0 44-45 34.746810107580686 36.0 36.0 36.0 32.0 36.0 46-47 34.64886164623468 36.0 36.0 36.0 32.0 36.0 48-49 34.56454841130848 36.0 36.0 36.0 32.0 36.0 50-51 34.73430072554416 36.0 36.0 36.0 32.0 36.0 52-53 34.58193645233925 36.0 36.0 36.0 32.0 36.0 54-55 34.579559669752314 36.0 36.0 36.0 32.0 36.0 56-57 34.41093319989992 36.0 36.0 36.0 32.0 36.0 58-59 34.234425819364525 36.0 36.0 36.0 32.0 36.0 60-61 34.3677758318739 36.0 36.0 36.0 32.0 36.0 62-63 34.24480860645484 36.0 36.0 36.0 32.0 36.0 64-65 34.23617713284963 36.0 36.0 36.0 32.0 36.0 66-67 34.182636977733296 36.0 36.0 36.0 32.0 36.0 68-69 34.37628221165875 36.0 36.0 36.0 32.0 36.0 70-71 34.06567425569177 36.0 36.0 36.0 32.0 36.0 72-73 34.15111333500125 36.0 36.0 36.0 32.0 36.0 74-75 34.08696196196196 36.0 36.0 36.0 32.0 36.0 76-77 34.0035035035035 36.0 36.0 36.0 32.0 36.0 78-79 33.95595595595596 36.0 36.0 36.0 29.5 36.0 80-81 34.00688773880263 36.0 36.0 36.0 32.0 36.0 82-83 33.8722846039658 36.0 36.0 36.0 29.5 36.0 84-85 33.94703230653644 36.0 36.0 36.0 29.5 36.0 86-87 33.92261457550714 36.0 36.0 36.0 32.0 36.0 88-89 33.88642624593038 36.0 36.0 36.0 27.0 36.0 90-91 33.77710707487837 36.0 36.0 36.0 27.0 36.0 92-93 33.7718045112782 36.0 36.0 36.0 27.0 36.0 94-95 33.89724310776943 36.0 36.0 36.0 27.0 36.0 96-97 33.73217153971956 36.0 36.0 36.0 27.0 36.0 98-99 33.58991145792315 36.0 36.0 36.0 27.0 36.0 100-101 32.86764747467983 36.0 34.0 36.0 20.5 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.0 11101 2 0.0 11101 3 0.0 11101 4 0.0 11101 5 0.0 11101 6 0.0 11101 7 0.0 11101 8 0.0 11101 9 0.0 11101 10-11 0.0 11101 12-13 0.0 11101 14-15 0.0 11101 16-17 0.0 11101 18-19 0.0 11101 20-21 0.0 11101 22-23 0.0 11101 24-25 0.0 11101 26-27 0.0 11101 28-29 0.0 11101 30-31 0.0 11101 32-33 0.0 11101 34-35 0.0 11101 36-37 0.0 11101 38-39 0.0 11101 40-41 0.0 11101 42-43 0.0 11101 44-45 0.0 11101 46-47 0.0 11101 48-49 0.0 11101 50-51 0.0 11101 52-53 0.0 11101 54-55 0.0 11101 56-57 0.0 11101 58-59 0.0 11101 60-61 0.0 11101 62-63 0.0 11101 64-65 0.0 11101 66-67 0.0 11101 68-69 0.0 11101 70-71 0.0 11101 72-73 0.0 11101 74-75 0.0 11101 76-77 0.0 11101 78-79 0.0 11101 80-81 0.0 11101 82-83 0.0 11101 84-85 0.0 11101 86-87 0.0 11101 88-89 0.0 11101 90-91 0.0 11101 92-93 0.0 11101 94-95 0.0 11101 96-97 0.0 11101 98-99 0.0 11101 100-101 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 2.0 18 0.0 19 1.0 20 3.0 21 2.0 22 4.0 23 5.0 24 6.0 25 27.0 26 22.0 27 42.0 28 59.0 29 78.0 30 104.0 31 145.0 32 199.0 33 280.0 34 655.0 35 2365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.03225806451613 12.42810702675669 16.579144786196547 41.96049012253063 2 25.78144536134033 18.854713678419603 29.557389347336834 25.806451612903224 3 26.431607901975497 23.58089522380595 21.8304576144036 28.157039259814955 4 27.056764191047762 29.75743935983996 17.254313578394598 25.93148287071768 5 28.557139284821204 29.707426856714182 18.554638659664917 23.1807951987997 6 23.736097067745195 31.445904954499493 20.348837209302324 24.469160768452983 7 21.48037009252313 15.703925981495374 36.884221055263815 25.93148287071768 8 22.755688922230558 20.255063765941486 25.35633908477119 31.632908227056767 9 24.58114528632158 19.079769942485623 26.431607901975497 29.9074768692173 10-11 26.76919229807452 27.069267316829208 18.35458864716179 27.806951737934483 12-13 25.03125781445361 20.230057514378593 25.44386096524131 29.294823705926483 14-15 25.64391097774444 23.068267066766694 24.10602650662666 27.181795448862218 16-17 26.669167291822955 22.980745186296573 23.10577644411103 27.24431107776944 18-19 26.456614153538382 21.94298574643661 24.85621405351338 26.744186046511626 20-21 26.04401100275069 24.056014003500874 23.1807951987997 26.71917979494874 22-23 26.78169542385596 23.918479619904975 22.918229557389346 26.38159539884971 24-25 26.344086021505376 23.85596399099775 23.243310827706924 26.556639159789945 26-27 26.231557889472366 23.293323330832706 23.10577644411103 27.369342335583895 28-29 25.456364091022753 24.543635908977244 23.393348337084273 26.60665166291573 30-31 25.618904726181547 23.093273318329583 23.868467116779193 27.419354838709676 32-33 25.531382845711427 24.18104526131533 23.280820205051263 27.00675168792198 34-35 26.006501625406354 24.118529632408105 23.030757689422355 26.84421105276319 36-37 26.44411102775694 23.69342335583896 23.618404601150285 26.244061015253813 38-39 25.734650493935224 23.308740777791673 22.796048518194322 28.160560210078778 40-41 27.051025512756375 22.798899449724864 23.36168084042021 26.788394197098548 42-43 26.14133833646029 23.602251407129458 23.68980612883052 26.566604127579733 44-45 25.369026770077557 23.39254440830623 23.755316487365523 27.483112334250688 46-47 26.832624468351263 23.380035026269702 22.792094070552913 26.99524643482612 48-49 24.993745308981737 23.68026019514636 22.904678508881663 28.42131598699024 50-51 26.970227670753065 22.779584688516387 23.83037277958469 26.419814861145856 52-53 26.90768076057043 22.95471603702777 22.692019014260694 27.445584188141105 54-55 26.069552164123095 23.455091318488865 23.742807105328996 26.732549412059043 56-57 27.157868401300977 23.15486614961221 23.19239429572179 26.494871153365025 58-59 26.55741806354766 22.95471603702777 23.755316487365523 26.732549412059043 60-61 26.82011508631474 23.129847385539154 22.554415811858895 27.495621716287218 62-63 27.257943457593193 22.57943457593195 22.654490868151115 27.50813109832374 64-65 27.132849637227917 23.067300475356518 22.679509632224168 27.120340255191394 66-67 25.769326995246434 24.26820115086315 23.00475356517388 26.957718288716535 68-69 27.270452839629723 23.742807105328996 22.041531148361273 26.945208906680012 70-71 27.18288716537403 23.54265699274456 22.316737553164874 26.957718288716535 72-73 26.632474355766828 24.168126094570926 22.554415811858895 26.64498373780335 74-75 27.364864864864863 23.31081081081081 22.86036036036036 26.463963963963966 76-77 28.14064064064064 22.197197197197198 23.01051051051051 26.651651651651655 78-79 26.989489489489486 23.24824824824825 22.35985985985986 27.402402402402405 80-81 27.556000500563133 23.3012138655988 22.888249280440494 26.254536353397572 82-83 27.432077125328657 23.262802053336674 22.536622010767495 26.76849881056717 84-85 27.848735286751815 22.92762334084648 22.238918106686704 26.984723265715 86-87 26.37114951164538 22.727272727272727 23.002754820936637 27.89882294014525 88-89 26.872026045579766 23.065364387678436 22.664663160530928 27.39794640621087 90-91 26.425259992482147 22.95451697782233 23.518356095727352 27.10186693396817 92-93 26.8671679197995 23.521303258145362 22.493734335839598 27.11779448621554 94-95 26.917293233082706 23.05764411027569 22.54385964912281 27.481203007518793 96-97 27.809834420471653 23.68289011540391 21.889111891620672 26.61816357250376 98-99 25.927337398373986 22.789634146341463 23.47560975609756 27.807418699186993 100-101 28.845553822152887 9.68798751950078 27.659906396255852 33.806552262090484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.0 24 0.0 25 1.0 26 2.0 27 2.0 28 2.5 29 3.5 30 6.0 31 6.5 32 5.5 33 10.0 34 17.0 35 26.0 36 33.0 37 32.5 38 32.5 39 39.5 40 54.0 41 68.0 42 85.5 43 87.0 44 88.5 45 112.0 46 128.0 47 132.0 48 129.0 49 142.0 50 168.5 51 175.0 52 161.5 53 165.0 54 180.0 55 195.5 56 198.5 57 193.0 58 176.0 59 144.5 60 122.0 61 97.5 62 89.0 63 79.5 64 65.0 65 59.5 66 53.5 67 48.5 68 48.0 69 50.5 70 46.5 71 47.0 72 36.0 73 23.0 74 28.5 75 24.0 76 19.5 77 17.0 78 13.0 79 13.5 80 7.5 81 3.5 82 3.5 83 2.5 84 2.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.025 3 0.025 4 0.025 5 0.025 6 1.0999999999999999 7 0.025 8 0.025 9 0.025 10-11 0.025 12-13 0.025 14-15 0.025 16-17 0.025 18-19 0.025 20-21 0.025 22-23 0.025 24-25 0.025 26-27 0.025 28-29 0.025 30-31 0.025 32-33 0.025 34-35 0.025 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 34-35 1.0 36-37 0.0 38-39 1.0 40-41 0.0 42-43 1.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.0 82-83 1.0 84-85 0.0 86-87 0.0 88-89 2.0 90-91 1.0 92-93 0.0 94-95 2.0 96-97 18.0 98-99 325.0 100-101 3645.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.725 #Duplication Level Percentage of deduplicated Percentage of total 1 92.61504546707083 84.025 2 5.456048498208873 9.9 3 1.4053458252962248 3.8249999999999997 4 0.35822540644805734 1.3 5 0.055111600992008826 0.25 6 0.027555800496004413 0.15 7 0.055111600992008826 0.35000000000000003 8 0.027555800496004413 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGAT 8 0.2 No Hit TCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATG 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 13 (97% over 38bp) GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 6 0.15 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 5 0.125 No Hit ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.025 70-71 0.0 0.0 0.0 0.0 0.025 72-73 0.0 0.0 0.0 0.0 0.025 74-75 0.0 0.0 0.0 0.0 0.025 76-77 0.0 0.0 0.0 0.0 0.025 78-79 0.0 0.0 0.0 0.0 0.025 80-81 0.0 0.0 0.0 0.0 0.025 82-83 0.0 0.0 0.0 0.0 0.025 84-85 0.0 0.0 0.0 0.0 0.025 86-87 0.0 0.0 0.0 0.0 0.025 88-89 0.0 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207074 spots for SRR21853441.sra Written 1207074 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra Read 1207067 spots for SRR21853441.sra Written 1207067 spots for SRR21853441.sra SRR ids: ['SRR21853441.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rmt90lax SRR21853441.sra spots: 24141347 blocks: [[1, 1207067], [1207068, 2414134], [2414135, 3621201], [3621202, 4828268], [4828269, 6035335], [6035336, 7242402], [7242403, 8449469], [8449470, 9656536], [9656537, 10863603], [10863604, 12070670], [12070671, 13277737], [13277738, 14484804], [14484805, 15691871], [15691872, 16898938], [16898939, 18106005], [18106006, 19313072], [19313073, 20520139], [20520140, 21727206], [21727207, 22934273], [22934274, 24141347]] SRR21853441 file size 6594234 SRR21853441 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR21853441 SRR21853441_1.fastq Input file: SRR21853441_1.fastq trimmed: SRR21853441-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Fri Dec 6 14:52:13 2024 >> started Fri Dec 6 14:52:25 2024 >> done (12.405s) 24141347 reads processed; of these: 99 ( 0.00%) short reads filtered out after trimming by size control 104601 ( 0.43%) empty reads filtered out after trimming by size control 24036647 (99.57%) reads available; of these: 316 ( 0.00%) trimmed reads available after processing 24036331 (100.00%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 2 0.00% 20 3 0.00% 21 2 0.00% 22 3 0.00% 23 4 0.00% 24 0 0.00% 25 2 0.00% 26 7 0.00% 27 1 0.00% 28 1 0.00% 29 4 0.00% 30 2 0.00% 31 0 0.00% 32 3 0.00% 33 2 0.00% 34 2 0.00% 35 254 0.00% 36 214 0.00% 37 238 0.00% 38 214 0.00% 39 226 0.00% 40 265 0.00% 41 262 0.00% 42 287 0.00% 43 273 0.00% 44 255 0.00% 45 303 0.00% 46 287 0.00% 47 338 0.00% 48 301 0.00% 49 326 0.00% 50 297 0.00% 51 332 0.00% 52 338 0.00% 53 342 0.00% 54 334 0.00% 55 351 0.00% 56 360 0.00% 57 408 0.00% 58 420 0.00% 59 465 0.00% 60 492 0.00% 61 456 0.00% 62 501 0.00% 63 528 0.00% 64 495 0.00% 65 498 0.00% 66 501 0.00% 67 538 0.00% 68 596 0.00% 69 548 0.00% 70 586 0.00% 71 570 0.00% 72 601 0.00% 73 664 0.00% 74 635 0.00% 75 674 0.00% 76 703 0.00% 77 738 0.00% 78 736 0.00% 79 814 0.00% 80 755 0.00% 81 910 0.00% 82 875 0.00% 83 881 0.00% 84 978 0.00% 85 986 0.00% 86 1029 0.00% 87 1043 0.00% 88 1125 0.00% 89 1221 0.01% 90 1297 0.01% 91 4090 0.02% 92 2091 0.01% 93 2207 0.01% 94 2071 0.01% 95 4676 0.02% 96 22098 0.09% 97 105618 0.44% 98 355440 1.48% 99 1541112 6.41% 100 5189947 21.59% 101 16776593 69.80% 24036647 reads passed initial QC criterion=sequence-density sequence-density=0.78 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=21 prefix-density=0.78 prefix-fanout=2.0 sequence=TTCGCTATCGGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=15.35 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=1.4 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACA Started job on | Dec 06 14:52:42 Started mapping on | Dec 06 14:52:42 Finished on | Dec 06 14:53:27 Mapping speed, Million of reads per hour | 1922.93 Number of input reads | 24036647 Average input read length | 100 UNIQUE READS: Uniquely mapped reads number | 11401855 Uniquely mapped reads % | 47.44% Average mapped length | 100.22 Number of splices: Total | 3638780 Number of splices: Annotated (sjdb) | 3457234 Number of splices: GT/AG | 3584257 Number of splices: GC/AG | 44790 Number of splices: AT/AC | 2194 Number of splices: Non-canonical | 7539 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.00% Deletion average length | 1.56 Insertion rate per base | 0.00% Insertion average length | 1.52 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 5061649 % of reads mapped to multiple loci | 21.06% Number of reads mapped to too many loci | 6601321 % of reads mapped to too many loci | 27.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.51% % of reads unmapped: other | 2.54% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 7573143 7573143 7573143 N_multimapping 5061649 5061649 5061649 N_noFeature 936600 6111237 6085896 N_ambiguous 168800 15687 13147 UnstrandedReadsAssigned:10296455 PositiveStrandReadsAssigned:5274931 NegativeStrandReadsAssigned:5302812 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=100 echo kmer=95 SRR21853441 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR21853441-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,036,647 reads, 12,585,119 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,094 rounds 52973 SRR21853441.ke.tsv 35125 SRR21853441.se.tsv 88098 total ==> SRR21853441.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 42.326 5.5338 PNS24247 1044 945 15.1701 1.7567 PNS24249 1928 1829 112.919 6.75609 PNS24246 1044 945 15.1701 1.7567 PNS24248 1044 945 15.1701 1.7567 PNS24244 1471 1372 14.2447 1.13616 PNS24243 293 194 7 3.94856 KQK14069 1603 1504 534.709 38.9055 KQK14071 474 375 79.9401 23.3279 ==> SRR21853441.se.tsv <== BRADI_1g14170v3 660 BRADI_1g53295v3 39 BRADI_1g59795v3 122 BRADI_1g07683v3 0 BRADI_1g00485v3 44 BRADI_1g20270v3 1268 BRADI_1g74790v3 142 BRADI_1g09890v3 5 BRADI_1g77505v3 119 BRADI_1g48960v3 0 SRR21853441 completed mapping pipeline successfully