Starting /dee2/code/volunteer_pipeline.sh SRR21853485 current disk space = 1550495842304 free memory = 1603317524 SRR21853485 SRAfilesize fac651bd8cc8b544470884274b791efa SRR21853485.sra SRR21853485.sra file validated SRR21853485 is single end SRR21853485 is conventional basespace SRR21853485 read1 length is 44-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR21853485_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 44-101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.0705 37.0 37.0 37.0 25.0 37.0 2 34.79275 37.0 37.0 37.0 25.0 37.0 3 35.714 37.0 37.0 37.0 37.0 37.0 4 35.774 37.0 37.0 37.0 37.0 37.0 5 35.7975 37.0 37.0 37.0 37.0 37.0 6 35.9155 37.0 37.0 37.0 37.0 37.0 7 35.6615 37.0 37.0 37.0 37.0 37.0 8 35.7415 37.0 37.0 37.0 37.0 37.0 9 35.801 37.0 37.0 37.0 37.0 37.0 10-11 35.92175 37.0 37.0 37.0 37.0 37.0 12-13 35.90325 37.0 37.0 37.0 37.0 37.0 14-15 35.858000000000004 37.0 37.0 37.0 37.0 37.0 16-17 35.814 37.0 37.0 37.0 37.0 37.0 18-19 35.800250000000005 37.0 37.0 37.0 37.0 37.0 20-21 35.9035 37.0 37.0 37.0 37.0 37.0 22-23 35.879999999999995 37.0 37.0 37.0 37.0 37.0 24-25 35.68625 37.0 37.0 37.0 37.0 37.0 26-27 35.68075 37.0 37.0 37.0 37.0 37.0 28-29 35.70575 37.0 37.0 37.0 37.0 37.0 30-31 35.56275 37.0 37.0 37.0 37.0 37.0 32-33 35.635 37.0 37.0 37.0 37.0 37.0 34-35 35.67275 37.0 37.0 37.0 37.0 37.0 36-37 35.684250000000006 37.0 37.0 37.0 37.0 37.0 38-39 35.619 37.0 37.0 37.0 37.0 37.0 40-41 35.56875 37.0 37.0 37.0 37.0 37.0 42-43 35.477500000000006 37.0 37.0 37.0 37.0 37.0 44-45 35.53805370092523 37.0 37.0 37.0 37.0 37.0 46-47 35.561390347586894 37.0 37.0 37.0 37.0 37.0 48-49 35.48637159289822 37.0 37.0 37.0 37.0 37.0 50-51 35.59014753688422 37.0 37.0 37.0 37.0 37.0 52-53 35.53688422105526 37.0 37.0 37.0 37.0 37.0 54-55 35.518879719929984 37.0 37.0 37.0 37.0 37.0 56-57 35.55813953488372 37.0 37.0 37.0 37.0 37.0 58-59 35.47211802950737 37.0 37.0 37.0 37.0 37.0 60-61 35.47261815453864 37.0 37.0 37.0 37.0 37.0 62-63 35.54388597149287 37.0 37.0 37.0 37.0 37.0 64-65 35.51737934483621 37.0 37.0 37.0 37.0 37.0 66-67 35.447611902975744 37.0 37.0 37.0 37.0 37.0 68-69 35.48912228057014 37.0 37.0 37.0 37.0 37.0 70-71 35.444861215303824 37.0 37.0 37.0 31.0 37.0 72-73 35.3313328332083 37.0 37.0 37.0 37.0 37.0 74-75 35.40435108777194 37.0 37.0 37.0 37.0 37.0 76-77 35.28507126781695 37.0 37.0 37.0 37.0 37.0 78-79 35.32158039509878 37.0 37.0 37.0 37.0 37.0 80-81 35.36259064766192 37.0 37.0 37.0 37.0 37.0 82-83 35.352338084521136 37.0 37.0 37.0 31.0 37.0 84-85 35.257814453613406 37.0 37.0 37.0 25.0 37.0 86-87 35.3970992748187 37.0 37.0 37.0 37.0 37.0 88-89 35.41860465116279 37.0 37.0 37.0 37.0 37.0 90-91 35.325581395348834 37.0 37.0 37.0 31.0 37.0 92-93 35.30007501875469 37.0 37.0 37.0 37.0 37.0 94-95 35.26506626656664 37.0 37.0 37.0 31.0 37.0 96-97 35.31269356219265 37.0 37.0 37.0 37.0 37.0 98-99 35.35773024047511 37.0 37.0 37.0 37.0 37.0 100-101 35.2409849732997 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-11 0.0 1101 12-13 0.0 1101 14-15 0.0 1101 16-17 0.0 1101 18-19 0.0 1101 20-21 0.0 1101 22-23 0.0 1101 24-25 0.0 1101 26-27 0.0 1101 28-29 0.0 1101 30-31 0.0 1101 32-33 0.0 1101 34-35 0.0 1101 36-37 0.0 1101 38-39 0.0 1101 40-41 0.0 1101 42-43 0.0 1101 44-45 0.0 1101 46-47 0.0 1101 48-49 0.0 1101 50-51 0.0 1101 52-53 0.0 1101 54-55 0.0 1101 56-57 0.0 1101 58-59 0.0 1101 60-61 0.0 1101 62-63 0.0 1101 64-65 0.0 1101 66-67 0.0 1101 68-69 0.0 1101 70-71 0.0 1101 72-73 0.0 1101 74-75 0.0 1101 76-77 0.0 1101 78-79 0.0 1101 80-81 0.0 1101 82-83 0.0 1101 84-85 0.0 1101 86-87 0.0 1101 88-89 0.0 1101 90-91 0.0 1101 92-93 0.0 1101 94-95 0.0 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 0.0 22 3.0 23 1.0 24 3.0 25 5.0 26 15.0 27 17.0 28 34.0 29 58.0 30 69.0 31 107.0 32 134.0 33 182.0 34 280.0 35 523.0 36 2118.0 37 449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.95 14.2 15.725 41.125 2 25.791740562452496 20.977957942741323 30.048137826197113 23.18216366860907 3 27.0 22.225 23.549999999999997 27.224999999999998 4 27.200000000000003 29.349999999999998 17.325 26.125 5 26.424999999999997 31.45 20.599999999999998 21.525 6 22.525000000000002 32.425 22.650000000000002 22.400000000000002 7 20.9 16.175 37.1 25.825 8 22.400000000000002 21.65 25.0 30.95 9 22.6 20.974999999999998 28.599999999999998 27.825 10-11 25.387500000000003 27.650000000000002 20.349999999999998 26.6125 12-13 24.087500000000002 22.2125 26.1125 27.5875 14-15 24.087500000000002 24.462500000000002 24.4875 26.9625 16-17 24.637500000000003 23.9375 24.4875 26.937499999999996 18-19 25.0 25.2125 24.1125 25.674999999999997 20-21 25.174999999999997 24.3875 24.587500000000002 25.85 22-23 24.5375 24.95 24.0375 26.474999999999998 24-25 24.1375 24.1625 25.7625 25.937500000000004 26-27 24.837500000000002 23.875 25.0375 26.25 28-29 24.6875 24.4125 25.587500000000002 25.3125 30-31 25.0125 23.7625 24.0125 27.212500000000002 32-33 24.9875 25.3125 24.575 25.124999999999996 34-35 24.825 25.3125 23.599999999999998 26.2625 36-37 24.6 24.5125 24.4375 26.450000000000003 38-39 26.1 24.1875 23.575 26.137500000000003 40-41 24.8625 25.0625 24.1375 25.937500000000004 42-43 24.7375 24.95 24.0 26.3125 44-45 25.153144143017876 25.153144143017876 24.32804100512564 25.365670708838607 46-47 25.893973493373345 24.918729682420604 23.63090772693173 25.55638909727432 48-49 25.29382345586397 24.356089022255563 24.218554638659665 26.131532883220803 50-51 24.85621405351338 24.968742185546386 24.656164041010253 25.51887971992998 52-53 25.456364091022753 24.36859214803701 23.80595148787197 26.36909227306827 54-55 25.76894223555889 24.06851712928232 24.681170292573142 25.481370342585645 56-57 24.85621405351338 24.056014003500874 24.593648412103025 26.494123530882717 58-59 25.44386096524131 23.868467116779193 24.8062015503876 25.881470367591895 60-61 25.343835958989747 24.081020255063766 23.85596399099775 26.71917979494874 62-63 25.418854713678417 24.968742185546386 24.093523380845213 25.51887971992998 64-65 25.09377344336084 24.48112028007002 24.143535883970994 26.281570392598148 66-67 24.868717179294826 25.006251562890725 23.793448362090523 26.331582895723933 68-69 25.79394848712178 23.893473368342086 24.48112028007002 25.831457864466117 70-71 25.968992248062015 24.518629657414355 23.74343585896474 25.76894223555889 72-73 25.906476619154787 24.243560890222557 23.730932733183295 26.11902975743936 74-75 25.818954738684667 24.3935983995999 24.793698424606152 24.99374843710928 76-77 25.256314078519633 23.69342335583896 24.256064016004 26.79419854963741 78-79 24.868717179294826 24.681170292573142 23.393348337084273 27.056764191047762 80-81 25.29382345586397 24.943735933983497 24.131032758189548 25.63140785196299 82-83 25.993998499624904 23.755938984746187 23.843460865216304 26.406601650412604 84-85 24.493623405851466 25.23130782695674 23.793448362090523 26.481620405101275 86-87 25.531382845711427 24.85621405351338 23.393348337084273 26.219054763690924 88-89 26.019004751187797 24.081020255063766 23.080770192548137 26.819204801200303 90-91 25.481370342585645 24.781195298824706 23.655913978494624 26.081520380095025 92-93 26.006501625406354 23.93098274568642 24.593648412103025 25.468867216804203 94-95 24.15603900975244 25.18129532383096 23.88097024256064 26.78169542385596 96-97 26.06629143214509 23.30206378986867 24.515322076297686 26.116322701688553 98-99 25.31341015575535 23.97112827656072 24.54096492338863 26.174496644295303 100-101 26.18485277908991 11.368288458510472 29.822075263737997 32.62478349866163 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 1.0 26 2.5 27 3.5 28 3.5 29 4.5 30 5.5 31 8.0 32 10.0 33 11.5 34 25.0 35 34.0 36 42.0 37 51.0 38 61.0 39 72.0 40 97.0 41 125.0 42 142.0 43 150.0 44 161.0 45 169.0 46 172.0 47 176.0 48 156.5 49 165.5 50 162.5 51 141.5 52 139.0 53 132.0 54 119.0 55 111.0 56 113.0 57 113.0 58 114.5 59 100.0 60 87.5 61 82.0 62 72.0 63 63.0 64 62.0 65 59.0 66 53.0 67 58.5 68 63.5 69 57.0 70 43.0 71 40.0 72 32.5 73 22.5 74 23.0 75 20.5 76 17.5 77 20.0 78 14.5 79 6.0 80 2.0 81 1.5 82 2.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.325 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 44-45 1.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.0 96-97 19.0 98-99 335.0 100-101 3644.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.9 #Duplication Level Percentage of deduplicated Percentage of total 1 92.81485468245425 86.225 2 6.7814854682454255 12.6 3 0.34983853606027987 0.975 4 0.05382131324004305 0.2 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 994013 spots for SRR21853485.sra Written 994013 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra Read 993997 spots for SRR21853485.sra Written 993997 spots for SRR21853485.sra SRR ids: ['SRR21853485.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_p8wey5gm SRR21853485.sra spots: 19879956 blocks: [[1, 993997], [993998, 1987994], [1987995, 2981991], [2981992, 3975988], [3975989, 4969985], [4969986, 5963982], [5963983, 6957979], [6957980, 7951976], [7951977, 8945973], [8945974, 9939970], [9939971, 10933967], [10933968, 11927964], [11927965, 12921961], [12921962, 13915958], [13915959, 14909955], [14909956, 15903952], [15903953, 16897949], [16897950, 17891946], [17891947, 18885943], [18885944, 19879956]] SRR21853485 file size 5356101 SRR21853485 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR21853485 SRR21853485_1.fastq Input file: SRR21853485_1.fastq trimmed: SRR21853485-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Fri Dec 6 15:59:45 2024 >> started Fri Dec 6 15:59:55 2024 >> done (10.046s) 19879956 reads processed; of these: 19 ( 0.00%) short reads filtered out after trimming by size control 14379 ( 0.07%) empty reads filtered out after trimming by size control 19865558 (99.93%) reads available; of these: 308 ( 0.00%) trimmed reads available after processing 19865250 (100.00%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 0 0.00% 21 1 0.00% 22 2 0.00% 23 3 0.00% 24 3 0.00% 25 1 0.00% 26 4 0.00% 27 4 0.00% 28 1 0.00% 29 2 0.00% 30 2 0.00% 31 2 0.00% 32 3 0.00% 33 5 0.00% 34 3 0.00% 35 65 0.00% 36 76 0.00% 37 67 0.00% 38 64 0.00% 39 76 0.00% 40 97 0.00% 41 67 0.00% 42 74 0.00% 43 72 0.00% 44 43 0.00% 45 73 0.00% 46 94 0.00% 47 106 0.00% 48 92 0.00% 49 114 0.00% 50 119 0.00% 51 102 0.00% 52 105 0.00% 53 92 0.00% 54 113 0.00% 55 93 0.00% 56 96 0.00% 57 113 0.00% 58 115 0.00% 59 110 0.00% 60 146 0.00% 61 135 0.00% 62 149 0.00% 63 118 0.00% 64 165 0.00% 65 134 0.00% 66 126 0.00% 67 153 0.00% 68 152 0.00% 69 163 0.00% 70 134 0.00% 71 140 0.00% 72 173 0.00% 73 156 0.00% 74 151 0.00% 75 180 0.00% 76 190 0.00% 77 202 0.00% 78 201 0.00% 79 257 0.00% 80 210 0.00% 81 226 0.00% 82 236 0.00% 83 218 0.00% 84 267 0.00% 85 290 0.00% 86 281 0.00% 87 294 0.00% 88 346 0.00% 89 355 0.00% 90 470 0.00% 91 2007 0.01% 92 756 0.00% 93 817 0.00% 94 1256 0.01% 95 4487 0.02% 96 24854 0.13% 97 92342 0.46% 98 352913 1.78% 99 1337325 6.73% 100 4526560 22.79% 101 13513548 68.03% 19865558 reads passed initial QC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=26 prefix-density=0.33 prefix-fanout=2.1 sequence=CCGTCAATTCCTTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=19.39 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=1.4 sequence=ACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGA Started job on | Dec 06 16:00:13 Started mapping on | Dec 06 16:00:14 Finished on | Dec 06 16:00:49 Mapping speed, Million of reads per hour | 2043.31 Number of input reads | 19865558 Average input read length | 100 UNIQUE READS: Uniquely mapped reads number | 15640642 Uniquely mapped reads % | 78.73% Average mapped length | 100.27 Number of splices: Total | 5519907 Number of splices: Annotated (sjdb) | 5217893 Number of splices: GT/AG | 5447867 Number of splices: GC/AG | 61607 Number of splices: AT/AC | 3071 Number of splices: Non-canonical | 7362 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.00% Deletion average length | 1.67 Insertion rate per base | 0.00% Insertion average length | 1.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1564659 % of reads mapped to multiple loci | 7.88% Number of reads mapped to too many loci | 1841041 % of reads mapped to too many loci | 9.27% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.69% % of reads unmapped: other | 1.44% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2660257 2660257 2660257 N_multimapping 1564659 1564659 1564659 N_noFeature 847055 8151475 8133136 N_ambiguous 235273 15422 18356 UnstrandedReadsAssigned:14558314 PositiveStrandReadsAssigned:7473745 NegativeStrandReadsAssigned:7489150 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=100 echo kmer=95 SRR21853485 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR21853485-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,865,558 reads, 15,442,303 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,180 rounds 52973 SRR21853485.ke.tsv 35125 SRR21853485.se.tsv 88098 total ==> SRR21853485.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0.00124216 0.000161384 PNS24247 1044 945 65.978 7.59235 PNS24249 1928 1829 173.835 10.3355 PNS24246 1044 945 65.978 7.59235 PNS24248 1044 945 65.978 7.59235 PNS24244 1471 1372 66.2293 5.24934 PNS24243 293 194 18 10.0897 KQK14069 1603 1504 3652.83 264.113 KQK14071 474 375 691.286 200.463 ==> SRR21853485.se.tsv <== BRADI_1g14170v3 4836 BRADI_1g53295v3 263 BRADI_1g59795v3 354 BRADI_1g07683v3 0 BRADI_1g00485v3 26 BRADI_1g20270v3 186 BRADI_1g74790v3 399 BRADI_1g09890v3 0 BRADI_1g77505v3 166 BRADI_1g48960v3 0 SRR21853485 completed mapping pipeline successfully