Starting /dee2/code/volunteer_pipeline.sh SRR21853506 current disk space = 1550460391424 free memory = 1370412964 SRR21853506 SRAfilesize e7c4478a880929cb630bef7a4fb5c353 SRR21853506.sra SRR21853506.sra file validated SRR21853506 is single end SRR21853506 is conventional basespace SRR21853506 read1 length is 94-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR21853506_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 94-101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.0025 37.0 37.0 37.0 25.0 37.0 2 34.76375 37.0 37.0 37.0 25.0 37.0 3 35.443 37.0 37.0 37.0 37.0 37.0 4 35.665 37.0 37.0 37.0 37.0 37.0 5 35.779 37.0 37.0 37.0 37.0 37.0 6 35.8805 37.0 37.0 37.0 37.0 37.0 7 35.51 37.0 37.0 37.0 37.0 37.0 8 35.791 37.0 37.0 37.0 37.0 37.0 9 35.8735 37.0 37.0 37.0 37.0 37.0 10-11 35.81575 37.0 37.0 37.0 37.0 37.0 12-13 35.73725 37.0 37.0 37.0 37.0 37.0 14-15 35.817750000000004 37.0 37.0 37.0 37.0 37.0 16-17 35.858999999999995 37.0 37.0 37.0 37.0 37.0 18-19 35.7465 37.0 37.0 37.0 37.0 37.0 20-21 35.646249999999995 37.0 37.0 37.0 37.0 37.0 22-23 35.692750000000004 37.0 37.0 37.0 37.0 37.0 24-25 35.6375 37.0 37.0 37.0 37.0 37.0 26-27 35.635000000000005 37.0 37.0 37.0 37.0 37.0 28-29 35.6245 37.0 37.0 37.0 37.0 37.0 30-31 35.58175 37.0 37.0 37.0 37.0 37.0 32-33 35.5175 37.0 37.0 37.0 37.0 37.0 34-35 35.5595 37.0 37.0 37.0 37.0 37.0 36-37 35.61525 37.0 37.0 37.0 37.0 37.0 38-39 35.491 37.0 37.0 37.0 37.0 37.0 40-41 35.380250000000004 37.0 37.0 37.0 37.0 37.0 42-43 35.558 37.0 37.0 37.0 37.0 37.0 44-45 35.39375 37.0 37.0 37.0 37.0 37.0 46-47 35.4055 37.0 37.0 37.0 37.0 37.0 48-49 35.409 37.0 37.0 37.0 31.0 37.0 50-51 35.41675 37.0 37.0 37.0 37.0 37.0 52-53 35.4435 37.0 37.0 37.0 37.0 37.0 54-55 35.42075 37.0 37.0 37.0 37.0 37.0 56-57 35.4015 37.0 37.0 37.0 37.0 37.0 58-59 35.40125 37.0 37.0 37.0 37.0 37.0 60-61 35.426 37.0 37.0 37.0 37.0 37.0 62-63 35.3425 37.0 37.0 37.0 37.0 37.0 64-65 35.37875 37.0 37.0 37.0 37.0 37.0 66-67 35.214 37.0 37.0 37.0 31.0 37.0 68-69 35.228 37.0 37.0 37.0 31.0 37.0 70-71 35.217749999999995 37.0 37.0 37.0 31.0 37.0 72-73 35.247249999999994 37.0 37.0 37.0 31.0 37.0 74-75 35.26225 37.0 37.0 37.0 31.0 37.0 76-77 35.178749999999994 37.0 37.0 37.0 25.0 37.0 78-79 35.2795 37.0 37.0 37.0 31.0 37.0 80-81 35.2415 37.0 37.0 37.0 31.0 37.0 82-83 35.20025 37.0 37.0 37.0 25.0 37.0 84-85 35.162 37.0 37.0 37.0 25.0 37.0 86-87 35.2005 37.0 37.0 37.0 31.0 37.0 88-89 35.233000000000004 37.0 37.0 37.0 31.0 37.0 90-91 35.19625 37.0 37.0 37.0 25.0 37.0 92-93 35.22725 37.0 37.0 37.0 31.0 37.0 94-95 35.19578375843961 37.0 37.0 37.0 31.0 37.0 96-97 35.201373083939366 37.0 37.0 37.0 31.0 37.0 98-99 35.13101082122915 37.0 37.0 37.0 25.0 37.0 100-101 35.15112972348236 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-11 0.0 1101 12-13 0.0 1101 14-15 0.0 1101 16-17 0.0 1101 18-19 0.0 1101 20-21 0.0 1101 22-23 0.0 1101 24-25 0.0 1101 26-27 0.0 1101 28-29 0.0 1101 30-31 0.0 1101 32-33 0.0 1101 34-35 0.0 1101 36-37 0.0 1101 38-39 0.0 1101 40-41 0.0 1101 42-43 0.0 1101 44-45 0.0 1101 46-47 0.0 1101 48-49 0.0 1101 50-51 0.0 1101 52-53 0.0 1101 54-55 0.0 1101 56-57 0.0 1101 58-59 0.0 1101 60-61 0.0 1101 62-63 0.0 1101 64-65 0.0 1101 66-67 0.0 1101 68-69 0.0 1101 70-71 0.0 1101 72-73 0.0 1101 74-75 0.0 1101 76-77 0.0 1101 78-79 0.0 1101 80-81 0.0 1101 82-83 0.0 1101 84-85 0.0 1101 86-87 0.0 1101 88-89 0.0 1101 90-91 0.0 1101 92-93 0.0 1101 94-95 0.0 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 4.0 23 4.0 24 11.0 25 9.0 26 18.0 27 33.0 28 43.0 29 49.0 30 71.0 31 86.0 32 150.0 33 210.0 34 278.0 35 537.0 36 2128.0 37 368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.75 13.8 18.55 37.9 2 25.400457665903893 20.39155860666158 30.714467327739637 23.49351639969489 3 26.85 23.775 23.849999999999998 25.525 4 26.700000000000003 29.825000000000003 18.825 24.65 5 27.775 32.15 20.525 19.55 6 22.225 32.5 21.175 24.099999999999998 7 20.0 16.225 38.5 25.275 8 22.900000000000002 21.7 24.375 31.025000000000002 9 22.125 21.325 28.199999999999996 28.349999999999998 10-11 24.75 29.1375 20.6375 25.474999999999998 12-13 23.7625 22.3375 25.974999999999998 27.925 14-15 24.775 24.3 25.2875 25.637500000000003 16-17 25.137500000000003 23.8875 24.175 26.8 18-19 24.762500000000003 24.3875 24.45 26.400000000000002 20-21 24.6 24.85 24.837500000000002 25.7125 22-23 24.5625 24.825 24.762500000000003 25.85 24-25 24.3 24.0 24.975 26.724999999999998 26-27 25.650000000000002 25.2375 23.9375 25.174999999999997 28-29 25.275 25.674999999999997 24.099999999999998 24.95 30-31 25.25 24.9125 24.349999999999998 25.4875 32-33 23.7875 25.35 25.5375 25.324999999999996 34-35 25.2375 23.6875 23.9875 27.0875 36-37 24.025 24.5625 25.074999999999996 26.337500000000002 38-39 24.45 24.55 24.9375 26.0625 40-41 24.575 24.2625 24.975 26.187500000000004 42-43 24.9375 23.9125 24.625 26.525 44-45 24.349999999999998 25.637500000000003 23.974999999999998 26.0375 46-47 25.3125 25.412499999999998 24.6125 24.6625 48-49 25.2 25.0625 24.325 25.412499999999998 50-51 25.4 24.462500000000002 24.6625 25.474999999999998 52-53 24.65 25.0125 24.85 25.4875 54-55 25.0625 24.1125 25.025 25.8 56-57 25.6125 24.25 25.0625 25.074999999999996 58-59 25.650000000000002 24.15 23.724999999999998 26.474999999999998 60-61 24.9 25.25 25.137500000000003 24.712500000000002 62-63 25.5125 25.4375 24.6125 24.4375 64-65 25.45 25.137500000000003 23.474999999999998 25.937500000000004 66-67 24.6125 25.2 24.2375 25.95 68-69 24.887500000000003 25.8 24.525 24.7875 70-71 25.2625 24.2875 24.1375 26.3125 72-73 24.775 25.7 23.400000000000002 26.125 74-75 25.525 25.15 23.525 25.8 76-77 25.2125 24.05 24.6 26.137500000000003 78-79 25.074999999999996 24.587500000000002 24.4375 25.900000000000002 80-81 25.174999999999997 24.725 23.9875 26.1125 82-83 25.7875 24.3625 24.5375 25.3125 84-85 25.387500000000003 24.099999999999998 24.337500000000002 26.174999999999997 86-87 25.1875 25.624999999999996 24.7875 24.4 88-89 25.887500000000003 25.025 23.9875 25.1 90-91 25.4875 24.375 24.6875 25.45 92-93 24.837500000000002 24.7 24.2375 26.224999999999998 94-95 25.115639454931866 24.52806600825103 24.415551943992998 25.9407425928241 96-97 25.13134851138354 24.080560420315237 25.2064048036027 25.581686264698522 98-99 24.93946731234867 24.09838154708806 25.321779023830764 25.640372116732507 100-101 26.694110196326786 11.510449651678277 29.829005699810008 31.966434452184927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 1.0 3 0.5 4 0.0 5 0.5 6 1.0 7 0.5 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 0.5 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 0.5 24 0.5 25 0.5 26 2.0 27 4.0 28 4.0 29 5.0 30 7.5 31 11.0 32 14.5 33 13.5 34 17.5 35 26.0 36 38.5 37 53.0 38 66.0 39 93.0 40 110.0 41 124.0 42 147.5 43 154.5 44 150.5 45 178.0 46 200.5 47 191.0 48 186.5 49 171.5 50 148.5 51 146.5 52 139.5 53 122.0 54 126.5 55 118.5 56 102.0 57 95.5 58 90.0 59 83.0 60 76.5 61 66.0 62 66.0 63 72.5 64 60.0 65 54.5 66 53.0 67 55.5 68 63.5 69 49.5 70 35.0 71 38.0 72 38.0 73 35.0 74 28.0 75 15.5 76 9.5 77 10.5 78 10.5 79 6.0 80 4.0 81 3.5 82 1.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.675 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 94 1.0 95 1.0 96 2.0 97 27.0 98 91.0 99 263.0 100 914.0 101 2701.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.7 #Duplication Level Percentage of deduplicated Percentage of total 1 94.02347918890075 88.1 2 5.389541088580576 10.100000000000001 3 0.5602988260405549 1.575 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.026680896478121666 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT 9 0.22499999999999998 TruSeq Adapter, Index 8 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959024 spots for SRR21853506.sra Written 959024 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra Read 959016 spots for SRR21853506.sra Written 959016 spots for SRR21853506.sra SRR ids: ['SRR21853506.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rjyl12_w SRR21853506.sra spots: 19180328 blocks: [[1, 959016], [959017, 1918032], [1918033, 2877048], [2877049, 3836064], [3836065, 4795080], [4795081, 5754096], [5754097, 6713112], [6713113, 7672128], [7672129, 8631144], [8631145, 9590160], [9590161, 10549176], [10549177, 11508192], [11508193, 12467208], [12467209, 13426224], [13426225, 14385240], [14385241, 15344256], [15344257, 16303272], [16303273, 17262288], [17262289, 18221304], [18221305, 19180328]] SRR21853506 file size 5166630 SRR21853506 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR21853506 SRR21853506_1.fastq Input file: SRR21853506_1.fastq trimmed: SRR21853506-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Fri Dec 6 16:34:18 2024 >> started Fri Dec 6 16:34:28 2024 >> done (10.530s) 19180328 reads processed; of these: 23 ( 0.00%) short reads filtered out after trimming by size control 63677 ( 0.33%) empty reads filtered out after trimming by size control 19116628 (99.67%) reads available; of these: 359 ( 0.00%) trimmed reads available after processing 19116269 (100.00%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 0 0.00% 21 0 0.00% 22 1 0.00% 23 4 0.00% 24 3 0.00% 25 2 0.00% 26 8 0.00% 27 2 0.00% 28 2 0.00% 29 3 0.00% 30 1 0.00% 31 8 0.00% 32 6 0.00% 33 4 0.00% 34 5 0.00% 35 83 0.00% 36 88 0.00% 37 98 0.00% 38 88 0.00% 39 91 0.00% 40 97 0.00% 41 78 0.00% 42 96 0.00% 43 110 0.00% 44 91 0.00% 45 118 0.00% 46 116 0.00% 47 101 0.00% 48 115 0.00% 49 91 0.00% 50 109 0.00% 51 120 0.00% 52 129 0.00% 53 132 0.00% 54 125 0.00% 55 119 0.00% 56 146 0.00% 57 138 0.00% 58 162 0.00% 59 180 0.00% 60 193 0.00% 61 217 0.00% 62 171 0.00% 63 189 0.00% 64 163 0.00% 65 173 0.00% 66 205 0.00% 67 168 0.00% 68 198 0.00% 69 214 0.00% 70 217 0.00% 71 239 0.00% 72 244 0.00% 73 232 0.00% 74 249 0.00% 75 286 0.00% 76 283 0.00% 77 256 0.00% 78 310 0.00% 79 344 0.00% 80 323 0.00% 81 321 0.00% 82 364 0.00% 83 412 0.00% 84 378 0.00% 85 454 0.00% 86 419 0.00% 87 484 0.00% 88 511 0.00% 89 562 0.00% 90 683 0.00% 91 1460 0.01% 92 718 0.00% 93 865 0.00% 94 1585 0.01% 95 4790 0.03% 96 25910 0.14% 97 90430 0.47% 98 350387 1.83% 99 1295740 6.78% 100 4412472 23.08% 101 12919938 67.58% 19116628 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=2.75 fanout-score-rank=13 prefix-density=0.42 prefix-fanout=1.9 sequence=CCTTTCCAGGGCCTCAAGTCCACCGCCGGCCTCCCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=8.95 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=1.0 sequence=GAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGG Started job on | Dec 06 16:34:49 Started mapping on | Dec 06 16:34:49 Finished on | Dec 06 16:35:14 Mapping speed, Million of reads per hour | 2752.79 Number of input reads | 19116628 Average input read length | 100 UNIQUE READS: Uniquely mapped reads number | 17323564 Uniquely mapped reads % | 90.62% Average mapped length | 100.19 Number of splices: Total | 6442740 Number of splices: Annotated (sjdb) | 6127402 Number of splices: GT/AG | 6353055 Number of splices: GC/AG | 76197 Number of splices: AT/AC | 3377 Number of splices: Non-canonical | 10111 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.01% Deletion average length | 2.23 Insertion rate per base | 0.01% Insertion average length | 2.01 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 825531 % of reads mapped to multiple loci | 4.32% Number of reads mapped to too many loci | 614797 % of reads mapped to too many loci | 3.22% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.40% % of reads unmapped: other | 0.44% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 967533 967533 967533 N_multimapping 825531 825531 825531 N_noFeature 707722 8814352 8983948 N_ambiguous 265010 16473 17828 UnstrandedReadsAssigned:16350832 PositiveStrandReadsAssigned:8492739 NegativeStrandReadsAssigned:8321788 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=100 echo kmer=95 SRR21853506 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR21853506-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,116,628 reads, 16,970,456 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,161 rounds 52973 SRR21853506.ke.tsv 35125 SRR21853506.se.tsv 88098 total ==> SRR21853506.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 56.2096 6.74706 PNS24247 1044 945 44.7574 4.75842 PNS24249 1928 1829 102.732 5.64315 PNS24246 1044 945 44.7574 4.75842 PNS24248 1044 945 44.7574 4.75842 PNS24244 1471 1372 17.7861 1.30244 PNS24243 293 194 7 3.62515 KQK14069 1603 1504 1378.86 92.1088 KQK14071 474 375 285.603 76.5174 ==> SRR21853506.se.tsv <== BRADI_1g14170v3 1753 BRADI_1g53295v3 97 BRADI_1g59795v3 245 BRADI_1g07683v3 0 BRADI_1g00485v3 79 BRADI_1g20270v3 1945 BRADI_1g74790v3 116 BRADI_1g09890v3 4 BRADI_1g77505v3 275 BRADI_1g48960v3 0 SRR21853506 completed mapping pipeline successfully