Starting /dee2/code/volunteer_pipeline.sh SRR21853538 current disk space = 1550608998400 free memory = 1598060072 SRR21853538 SRAfilesize 3dd39a7b81fbebaa086e1629b6ef25c5 SRR21853538.sra SRR21853538.sra file validated SRR21853538 is single end SRR21853538 is conventional basespace SRR21853538 read1 length is 35-101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR21853538_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-101 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.16675 37.0 37.0 37.0 25.0 37.0 2 34.65425 37.0 37.0 37.0 25.0 37.0 3 35.68675 37.0 37.0 37.0 37.0 37.0 4 35.79125 37.0 37.0 37.0 37.0 37.0 5 35.86675 37.0 37.0 37.0 37.0 37.0 6 35.82375 37.0 37.0 37.0 37.0 37.0 7 35.74475 37.0 37.0 37.0 37.0 37.0 8 35.85975 37.0 37.0 37.0 37.0 37.0 9 35.87525 37.0 37.0 37.0 37.0 37.0 10-11 35.98475 37.0 37.0 37.0 37.0 37.0 12-13 35.98625 37.0 37.0 37.0 37.0 37.0 14-15 35.985 37.0 37.0 37.0 37.0 37.0 16-17 35.923249999999996 37.0 37.0 37.0 37.0 37.0 18-19 35.914249999999996 37.0 37.0 37.0 37.0 37.0 20-21 36.04025 37.0 37.0 37.0 37.0 37.0 22-23 35.92225 37.0 37.0 37.0 37.0 37.0 24-25 35.8455 37.0 37.0 37.0 37.0 37.0 26-27 35.82875 37.0 37.0 37.0 37.0 37.0 28-29 35.6455 37.0 37.0 37.0 37.0 37.0 30-31 35.77275 37.0 37.0 37.0 37.0 37.0 32-33 35.7855 37.0 37.0 37.0 37.0 37.0 34-35 35.681 37.0 37.0 37.0 37.0 37.0 36-37 35.677419354838705 37.0 37.0 37.0 37.0 37.0 38-39 35.59064766191548 37.0 37.0 37.0 37.0 37.0 40-41 35.61565391347837 37.0 37.0 37.0 37.0 37.0 42-43 35.555638909727435 37.0 37.0 37.0 37.0 37.0 44-45 35.21580395098775 37.0 37.0 37.0 31.0 37.0 46-47 35.5280140070035 37.0 37.0 37.0 37.0 37.0 48-49 35.5440220110055 37.0 37.0 37.0 37.0 37.0 50-51 35.60763168674655 37.0 37.0 37.0 37.0 37.0 52-53 35.635226419814856 37.0 37.0 37.0 37.0 37.0 54-55 35.62646985238929 37.0 37.0 37.0 37.0 37.0 56-57 35.518388791593694 37.0 37.0 37.0 37.0 37.0 58-59 35.47485614210658 37.0 37.0 37.0 37.0 37.0 60-61 35.416562421816366 37.0 37.0 37.0 37.0 37.0 62-63 35.30347760820615 37.0 37.0 37.0 31.0 37.0 64-65 35.4315736802602 37.0 37.0 37.0 31.0 37.0 66-67 35.25118839129347 37.0 37.0 37.0 31.0 37.0 68-69 34.87740805604203 37.0 37.0 37.0 25.0 37.0 70-71 34.890167625719286 37.0 37.0 37.0 25.0 37.0 72-73 35.36846057571965 37.0 37.0 37.0 37.0 37.0 74-75 35.479349186483105 37.0 37.0 37.0 37.0 37.0 76-77 35.33141426783479 37.0 37.0 37.0 37.0 37.0 78-79 35.48060075093868 37.0 37.0 37.0 37.0 37.0 80-81 35.495926869026945 37.0 37.0 37.0 37.0 37.0 82-83 35.43940911367051 37.0 37.0 37.0 37.0 37.0 84-85 35.35678517776665 37.0 37.0 37.0 37.0 37.0 86-87 35.37305958938407 37.0 37.0 37.0 37.0 37.0 88-89 35.322063611319805 37.0 37.0 37.0 37.0 37.0 90-91 35.4095201422128 37.0 37.0 37.0 37.0 37.0 92-93 35.38315137631099 37.0 37.0 37.0 37.0 37.0 94-95 35.42082763941692 37.0 37.0 37.0 31.0 37.0 96-97 35.34501815720069 37.0 37.0 37.0 37.0 37.0 98-99 35.436458333333334 37.0 37.0 37.0 37.0 37.0 100-101 35.3517106645138 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-11 0.0 1101 12-13 0.0 1101 14-15 0.0 1101 16-17 0.0 1101 18-19 0.0 1101 20-21 0.0 1101 22-23 0.0 1101 24-25 0.0 1101 26-27 0.0 1101 28-29 0.0 1101 30-31 0.0 1101 32-33 0.0 1101 34-35 0.0 1101 36-37 0.0 1101 38-39 0.0 1101 40-41 0.0 1101 42-43 0.0 1101 44-45 0.0 1101 46-47 0.0 1101 48-49 0.0 1101 50-51 0.0 1101 52-53 0.0 1101 54-55 0.0 1101 56-57 0.0 1101 58-59 0.0 1101 60-61 0.0 1101 62-63 0.0 1101 64-65 0.0 1101 66-67 0.0 1101 68-69 0.0 1101 70-71 0.0 1101 72-73 0.0 1101 74-75 0.0 1101 76-77 0.0 1101 78-79 0.0 1101 80-81 0.0 1101 82-83 0.0 1101 84-85 0.0 1101 86-87 0.0 1101 88-89 0.0 1101 90-91 0.0 1101 92-93 0.0 1101 94-95 0.0 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 2.0 23 2.0 24 4.0 25 11.0 26 16.0 27 22.0 28 35.0 29 35.0 30 72.0 31 91.0 32 110.0 33 192.0 34 343.0 35 517.0 36 2069.0 37 476.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.65816454113528 11.127781945486372 15.57889472368092 40.63515878969742 2 27.300380228136884 21.698352344740176 27.224334600760457 23.776932826362483 3 27.53188297074269 22.755688922230558 22.83070767691923 26.881720430107524 4 27.28182045511378 28.457114278569644 16.379094773693424 27.881970492623154 5 31.007751937984494 28.75718929732433 18.27956989247312 21.955488872218055 6 25.531382845711427 29.732433108277068 20.030007501875467 24.706176544136035 7 19.754938734683673 18.204551137784446 36.159039759939986 25.881470367591895 8 22.20555138784696 22.55563890972743 24.63115778944736 30.607651912978245 9 24.781195298824706 19.629907476869217 26.38159539884971 29.207301825456366 10-11 27.881970492623154 26.731682920730183 18.854713678419603 26.531632908227053 12-13 25.23130782695674 20.655163790947736 24.406101525381345 29.707426856714182 14-15 24.55613903475869 24.081020255063766 22.73068267066767 28.632158039509875 16-17 26.331582895723933 22.568142035508878 22.255563890972745 28.844711177794448 18-19 25.531382845711427 22.80570142535634 24.318579644911228 27.344336084021002 20-21 27.019254813703427 22.53063265816454 25.381345336334082 25.068767191797946 22-23 25.6064016004001 26.39409852463116 22.118029507376843 25.881470367591895 24-25 26.04401100275069 23.13078269567392 23.280820205051263 27.544386096524132 26-27 25.6064016004001 22.405601400350086 22.768192048012004 29.21980495123781 28-29 27.33183295823956 23.13078269567392 23.40585146286572 26.131532883220803 30-31 24.456114028507127 22.330582645661416 25.51887971992998 27.694423605901473 32-33 24.981245311327832 24.55613903475869 22.443110777694425 28.019504876219052 34-35 26.456614153538382 24.668667166791696 23.20580145036259 25.668917229307326 36-37 27.206801700425103 23.40585146286572 22.468117029257314 26.91922980745186 38-39 25.806451612903224 23.330832708177045 24.468617154288573 26.39409852463116 40-41 28.00700175043761 24.568642160540136 22.605651412853213 24.81870467616904 42-43 25.143785946486624 25.44386096524131 23.455863965991497 25.95648912228057 44-45 25.831457864466117 22.73068267066767 23.50587646911728 27.93198299574894 46-47 27.33866933466733 22.52376188094047 22.736368184092047 27.40120060030015 48-49 24.862431215607803 24.149574787393696 24.84992496248124 26.138069034517258 50-51 26.303939962476548 21.651031894934334 24.978111319574733 27.066916823014388 52-53 25.331498623967974 22.016512384288216 22.829622216662496 29.82236677508131 54-55 27.5331498623968 22.992244183137352 23.54265699274456 25.93194896172129 56-57 25.081310983237426 22.629472104078058 24.36827620715537 27.920940705529144 58-59 25.819364523392547 22.91718789091819 24.180635476607456 27.08281210908181 60-61 27.107830873154864 22.066549912434326 24.055541656242184 26.770077558168627 62-63 25.869402051538653 21.065799349512133 25.606705028771582 27.45809357017763 64-65 27.145359019264447 22.404303227420566 25.01876407305479 25.431573680260193 66-67 25.83187390542907 25.631723792844635 22.54190642982237 25.99449587190393 68-69 25.081310983237426 24.956217162872154 22.71703777833375 27.24543407555667 70-71 27.858393795346508 23.430072554415812 22.629472104078058 26.08206154615962 72-73 28.6107634543179 22.290362953692114 22.14017521902378 26.958698372966204 74-75 29.036295369211512 21.664580725907385 23.028785982478098 26.270337922403 76-77 29.123904881101375 22.24030037546934 22.57822277847309 26.057571964956196 78-79 29.499374217772218 21.764705882352942 22.002503128911137 26.733416770963704 80-81 28.664413568656904 22.568531731130303 22.44335961947678 26.32369508073601 82-83 28.955933900851278 22.208312468703053 22.1206810215323 26.71507260891337 84-85 29.11867801702554 22.458688032048073 21.832749123685527 26.589884827240862 86-87 28.70555833750626 22.508763144717076 23.209814722083124 25.575863795693543 88-89 28.72526922113699 23.015276734285 22.138742799899823 26.120711244678184 90-91 28.628678772698812 22.642454602379463 22.705072010018785 26.023794614902947 92-93 29.156747274777594 23.117403834106 21.81430898383661 25.91153990727979 94-95 28.662739691690685 22.37122446421857 23.235994485524504 25.730041358566236 96-97 27.584044154540894 22.428499749121926 22.717009533366785 27.270446562970395 98-99 28.522380467955237 22.100712105798575 22.4059003051882 26.971007121057987 100-101 31.31595282433271 9.745499689633768 27.6691495965239 31.26939788950962 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 0.5 25 0.5 26 1.5 27 2.0 28 2.0 29 2.5 30 3.5 31 3.0 32 4.5 33 7.0 34 7.0 35 15.5 36 25.0 37 26.0 38 38.0 39 56.5 40 64.0 41 64.0 42 72.0 43 91.0 44 103.0 45 108.5 46 120.5 47 139.0 48 137.0 49 141.5 50 158.0 51 166.0 52 163.0 53 172.0 54 188.5 55 202.5 56 211.5 57 173.5 58 146.0 59 134.0 60 107.5 61 87.5 62 72.5 63 71.5 64 81.5 65 99.5 66 98.0 67 75.0 68 62.0 69 46.5 70 41.0 71 45.0 72 36.5 73 24.5 74 17.0 75 16.5 76 19.0 77 15.5 78 13.5 79 10.0 80 4.5 81 2.0 82 3.0 83 2.5 84 0.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 1.375 3 0.025 4 0.025 5 0.025 6 0.025 7 0.025 8 0.025 9 0.025 10-11 0.025 12-13 0.025 14-15 0.025 16-17 0.025 18-19 0.025 20-21 0.025 22-23 0.025 24-25 0.025 26-27 0.025 28-29 0.025 30-31 0.025 32-33 0.025 34-35 0.025 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 34-35 1.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.0 46-47 0.0 48-49 0.0 50-51 1.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 2.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0 82-83 0.0 84-85 0.0 86-87 1.0 88-89 0.0 90-91 2.0 92-93 1.0 94-95 1.0 96-97 29.0 98-99 320.0 100-101 3640.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.625 #Duplication Level Percentage of deduplicated Percentage of total 1 87.29689807976366 73.875 2 10.635155096011816 18.0 3 1.7134416543574595 4.35 4 0.1772525849335303 0.6 5 0.08862629246676515 0.375 6 0.029542097488921712 0.15 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.029542097488921712 0.75 >50 0.029542097488921712 1.9 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT 76 1.9 TruSeq Adapter, Index 1 (97% over 36bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCGCGTAT 30 0.75 TruSeq Adapter, Index 1 (97% over 36bp) CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT 6 0.15 No Hit CCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGAAT 5 0.125 No Hit GCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGCC 5 0.125 No Hit GTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 20 1.4800611E-5 95.72151 1 ATCGGAA 20 1.4800611E-5 95.72151 2 TCGGAAG 20 1.5780366E-5 94.525 3 GAGCACA 20 1.5780366E-5 94.525 9 AAGAGCA 30 1.17868825E-4 63.016663 7 AGAGCAC 30 1.17868825E-4 63.016663 8 GAAGAGC 35 2.526657E-4 54.01429 6 CGGAAGA 35 2.526657E-4 54.01429 4 GGAAGAG 35 2.526657E-4 54.01429 5 ACACGTC 20 5.198803E-4 47.2625 12-13 ATCTCGT 20 5.198803E-4 47.2625 42-43 CACACGT 20 5.198803E-4 47.2625 12-13 AGCACAC 20 5.198803E-4 47.2625 10-11 GCACACG 20 5.198803E-4 47.2625 10-11 CACGTCT 25 0.0015597243 37.809998 14-15 CTTGAAA 30 0.0038155 31.508331 62-63 >>END_MODULE Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879853 spots for SRR21853538.sra Written 879853 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra Read 879839 spots for SRR21853538.sra Written 879839 spots for SRR21853538.sra SRR ids: ['SRR21853538.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6nw8n5da SRR21853538.sra spots: 17596794 blocks: [[1, 879839], [879840, 1759678], [1759679, 2639517], [2639518, 3519356], [3519357, 4399195], [4399196, 5279034], [5279035, 6158873], [6158874, 7038712], [7038713, 7918551], [7918552, 8798390], [8798391, 9678229], [9678230, 10558068], [10558069, 11437907], [11437908, 12317746], [12317747, 13197585], [13197586, 14077424], [14077425, 14957263], [14957264, 15837102], [15837103, 16716941], [16716942, 17596794]] SRR21853538 file size 4739366 SRR21853538 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR21853538 SRR21853538_1.fastq Input file: SRR21853538_1.fastq trimmed: SRR21853538-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Fri Dec 6 17:25:07 2024 >> started Fri Dec 6 17:25:16 2024 >> done (9.353s) 17596794 reads processed; of these: 50 ( 0.00%) short reads filtered out after trimming by size control 425680 ( 2.42%) empty reads filtered out after trimming by size control 17171064 (97.58%) reads available; of these: 339 ( 0.00%) trimmed reads available after processing 17170725 (100.00%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 0 0.00% 20 3 0.00% 21 0 0.00% 22 6 0.00% 23 2 0.00% 24 2 0.00% 25 3 0.00% 26 2 0.00% 27 3 0.00% 28 1 0.00% 29 4 0.00% 30 2 0.00% 31 3 0.00% 32 4 0.00% 33 3 0.00% 34 6 0.00% 35 114 0.00% 36 129 0.00% 37 140 0.00% 38 134 0.00% 39 154 0.00% 40 170 0.00% 41 119 0.00% 42 163 0.00% 43 137 0.00% 44 136 0.00% 45 163 0.00% 46 135 0.00% 47 161 0.00% 48 172 0.00% 49 209 0.00% 50 229 0.00% 51 205 0.00% 52 188 0.00% 53 237 0.00% 54 259 0.00% 55 233 0.00% 56 211 0.00% 57 243 0.00% 58 259 0.00% 59 270 0.00% 60 314 0.00% 61 315 0.00% 62 330 0.00% 63 321 0.00% 64 366 0.00% 65 348 0.00% 66 373 0.00% 67 360 0.00% 68 388 0.00% 69 405 0.00% 70 450 0.00% 71 500 0.00% 72 506 0.00% 73 578 0.00% 74 573 0.00% 75 574 0.00% 76 622 0.00% 77 651 0.00% 78 647 0.00% 79 765 0.00% 80 754 0.00% 81 839 0.00% 82 937 0.01% 83 972 0.01% 84 1061 0.01% 85 1157 0.01% 86 1298 0.01% 87 1186 0.01% 88 1314 0.01% 89 1459 0.01% 90 1551 0.01% 91 4174 0.02% 92 2443 0.01% 93 2509 0.01% 94 2436 0.01% 95 4483 0.03% 96 18660 0.11% 97 75486 0.44% 98 266146 1.55% 99 1141037 6.65% 100 3767885 21.94% 101 11859275 69.07% 17171064 reads passed initial QC criterion=sequence-density sequence-density=0.73 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=18 prefix-density=0.74 prefix-fanout=2.1 sequence=GTGCCAGCAGCCGCGGTAA criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=16.22 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=1.0 sequence=TAACTTCGGGAGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGG Started job on | Dec 06 17:25:32 Started mapping on | Dec 06 17:25:32 Finished on | Dec 06 17:26:09 Mapping speed, Million of reads per hour | 1670.70 Number of input reads | 17171064 Average input read length | 100 UNIQUE READS: Uniquely mapped reads number | 8396270 Uniquely mapped reads % | 48.90% Average mapped length | 100.22 Number of splices: Total | 2715765 Number of splices: Annotated (sjdb) | 2572112 Number of splices: GT/AG | 2675273 Number of splices: GC/AG | 33115 Number of splices: AT/AC | 1342 Number of splices: Non-canonical | 6035 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.00% Deletion average length | 1.71 Insertion rate per base | 0.00% Insertion average length | 1.48 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 3632110 % of reads mapped to multiple loci | 21.15% Number of reads mapped to too many loci | 4356000 % of reads mapped to too many loci | 25.37% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.02% % of reads unmapped: other | 3.56% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5142684 5142684 5142684 N_multimapping 3632110 3632110 3632110 N_noFeature 721096 4504970 4503344 N_ambiguous 129380 10508 10917 UnstrandedReadsAssigned:7545794 PositiveStrandReadsAssigned:3880792 NegativeStrandReadsAssigned:3882009 Dataset is classified unstranded MeadianReadLen=101 20thPercentileLength=100 echo kmer=95 SRR21853538 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR21853538-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,171,064 reads, 9,067,294 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,049 rounds 52973 SRR21853538.ke.tsv 35125 SRR21853538.se.tsv 88098 total ==> SRR21853538.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 25.1518 4.1773 PNS24249 1928 1829 62.159 5.33395 PNS24246 1044 945 25.1518 4.1773 PNS24248 1044 945 25.1518 4.1773 PNS24244 1471 1372 10.3857 1.18807 PNS24243 293 194 11 8.89919 KQK14069 1603 1504 1682.59 175.586 KQK14071 474 375 321.39 134.512 ==> SRR21853538.se.tsv <== BRADI_1g14170v3 2197 BRADI_1g53295v3 50 BRADI_1g59795v3 83 BRADI_1g07683v3 0 BRADI_1g00485v3 47 BRADI_1g20270v3 763 BRADI_1g74790v3 79 BRADI_1g09890v3 4 BRADI_1g77505v3 90 BRADI_1g48960v3 0 SRR21853538 completed mapping pipeline successfully