Starting /dee2/code/volunteer_pipeline.sh SRR24040056 current disk space = 1548879925248 free memory = 1598624880 SRR24040056 SRAfilesize 80dd9b1717eeac661e62eb5cc19a2559 SRR24040056.sra SRR24040056.sra file validated SRR24040056 is paired end SRR24040056 is conventional basespace SRR24040056 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR24040056_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.74 37.0 37.0 37.0 37.0 37.0 2 36.346 37.0 37.0 37.0 37.0 37.0 3 36.136 37.0 37.0 37.0 37.0 37.0 4 36.5045 37.0 37.0 37.0 37.0 37.0 5 36.6015 37.0 37.0 37.0 37.0 37.0 6 35.424 37.0 37.0 37.0 37.0 37.0 7 36.622 37.0 37.0 37.0 37.0 37.0 8 36.586 37.0 37.0 37.0 37.0 37.0 9 33.3545 37.0 37.0 37.0 11.0 37.0 10-14 36.483999999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.386900000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.4089 37.0 37.0 37.0 37.0 37.0 25-29 36.3669 37.0 37.0 37.0 37.0 37.0 30-34 36.022400000000005 37.0 37.0 37.0 34.6 37.0 35-39 36.4074 37.0 37.0 37.0 37.0 37.0 40-44 36.2779 37.0 37.0 37.0 37.0 37.0 45-49 36.2416 37.0 37.0 37.0 37.0 37.0 50-54 36.3594 37.0 37.0 37.0 37.0 37.0 55-59 36.1057 37.0 37.0 37.0 37.0 37.0 60-64 36.0715 37.0 37.0 37.0 37.0 37.0 65-69 36.1432 37.0 37.0 37.0 37.0 37.0 70-74 36.2087 37.0 37.0 37.0 37.0 37.0 75-79 36.336 37.0 37.0 37.0 37.0 37.0 80-84 35.950300000000006 37.0 37.0 37.0 37.0 37.0 85-89 36.2377 37.0 37.0 37.0 37.0 37.0 90-94 36.2262 37.0 37.0 37.0 37.0 37.0 95-99 36.213 37.0 37.0 37.0 37.0 37.0 100-104 36.071600000000004 37.0 37.0 37.0 37.0 37.0 105-109 36.1465 37.0 37.0 37.0 37.0 37.0 110-114 36.0213 37.0 37.0 37.0 37.0 37.0 115-119 35.749700000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.62179999999999 37.0 37.0 37.0 34.6 37.0 125-129 35.8343 37.0 37.0 37.0 37.0 37.0 130-134 35.4503 37.0 37.0 37.0 34.6 37.0 135-139 35.544500000000006 37.0 37.0 37.0 37.0 37.0 140-144 35.2369 37.0 37.0 37.0 32.2 37.0 145-149 35.211 37.0 37.0 37.0 34.6 37.0 150 32.2365 37.0 37.0 37.0 11.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 0.0 22 0.0 23 3.0 24 3.0 25 6.0 26 6.0 27 17.0 28 23.0 29 21.0 30 35.0 31 53.0 32 54.0 33 85.0 34 163.0 35 541.0 36 2651.0 37 338.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 48.073073073073076 10.085085085085085 4.87987987987988 36.96196196196196 2 24.975 12.35 30.75 31.924999999999997 3 22.225 14.774999999999999 24.15 38.85 4 27.950000000000003 22.55 18.775 30.725 5 28.7 25.724999999999998 20.175 25.4 6 25.25 26.900000000000002 22.8 25.05 7 18.875 20.8 37.375 22.95 8 21.224999999999998 18.65 28.825 31.3 9 22.725 19.375 29.475 28.425 10-14 25.46 22.0 23.27 29.270000000000003 15-19 26.1 20.45 23.580000000000002 29.87 20-24 26.365 21.975 23.630000000000003 28.03 25-29 25.490000000000002 21.315 23.51 29.685 30-34 25.665 20.23 24.015 30.09 35-39 25.36 20.79 23.974999999999998 29.875 40-44 25.485000000000003 21.105 24.275 29.134999999999998 45-49 25.629999999999995 20.445 24.474999999999998 29.45 50-54 24.895 20.745 24.135 30.225 55-59 25.69 20.68 24.09 29.54 60-64 24.325 19.97 25.240000000000002 30.464999999999996 65-69 25.7 21.805 23.515 28.98 70-74 26.724999999999998 21.365000000000002 22.585 29.325000000000003 75-79 27.115000000000002 21.395 22.445 29.044999999999998 80-84 26.625 21.075 22.57 29.73 85-89 26.834999999999997 22.08 22.09 28.994999999999997 90-94 26.384999999999998 21.38 22.73 29.505 95-99 25.979999999999997 21.365000000000002 23.135 29.520000000000003 100-104 26.284999999999997 22.175 22.215 29.325000000000003 105-109 26.810000000000002 22.685 22.09 28.415000000000003 110-114 27.68 21.795 22.045 28.48 115-119 27.839999999999996 22.14 21.975 28.044999999999998 120-124 27.265 22.28 22.205 28.249999999999996 125-129 27.189999999999998 22.06 21.775 28.975 130-134 28.715000000000003 20.830000000000002 21.82 28.634999999999998 135-139 28.660000000000004 21.015 22.220000000000002 28.105000000000004 140-144 29.099999999999998 20.94 22.17 27.79 145-149 30.240000000000002 20.365 21.895 27.500000000000004 150 30.375000000000004 22.025 21.0 26.6 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.5 29 0.5 30 1.0 31 2.0 32 1.5 33 4.0 34 4.0 35 5.5 36 15.5 37 19.5 38 13.5 39 18.5 40 32.5 41 41.5 42 50.0 43 63.0 44 80.5 45 86.0 46 104.0 47 140.5 48 154.0 49 168.5 50 192.5 51 199.0 52 179.5 53 178.0 54 170.5 55 156.0 56 166.5 57 162.5 58 142.0 59 122.5 60 120.5 61 113.0 62 108.0 63 104.0 64 83.0 65 80.5 66 92.5 67 87.0 68 79.0 69 69.5 70 69.5 71 77.0 72 66.0 73 43.0 74 26.0 75 32.0 76 32.0 77 18.0 78 15.0 79 8.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.4 #Duplication Level Percentage of deduplicated Percentage of total 1 84.184350132626 63.475 2 9.217506631299734 13.900000000000002 3 3.0172413793103448 6.825 4 1.6578249336870028 5.0 5 0.43103448275862066 1.625 6 0.4641909814323607 2.1 7 0.3647214854111406 1.925 8 0.1989389920424403 1.2 9 0.2652519893899204 1.7999999999999998 >10 0.1989389920424403 2.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT 30 0.75 TruSeq Adapter, Index 20 (97% over 44bp) GTCAGTATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGC 13 0.325 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC 12 0.3 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 11 0.27499999999999997 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 10 0.25 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 10 0.25 No Hit GTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACAC 9 0.22499999999999998 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 9 0.22499999999999998 No Hit GTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCC 9 0.22499999999999998 No Hit CTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTG 9 0.22499999999999998 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 9 0.22499999999999998 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 9 0.22499999999999998 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 9 0.22499999999999998 No Hit GTCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACT 9 0.22499999999999998 No Hit ATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGC 8 0.2 No Hit GCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCC 8 0.2 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT 8 0.2 TruSeq Adapter, Index 20 (97% over 45bp) GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 8 0.2 No Hit CCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCGGGCCGGACGGG 8 0.2 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCC 7 0.17500000000000002 No Hit GTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC 7 0.17500000000000002 No Hit CTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGGCTCCAGCTATC 7 0.17500000000000002 No Hit GTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGC 7 0.17500000000000002 No Hit CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCC 7 0.17500000000000002 No Hit GCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGCC 7 0.17500000000000002 No Hit CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC 7 0.17500000000000002 No Hit CCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGT 7 0.17500000000000002 No Hit GTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAG 7 0.17500000000000002 No Hit CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC 7 0.17500000000000002 No Hit CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC 7 0.17500000000000002 No Hit CTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGG 6 0.15 No Hit GTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAG 6 0.15 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 6 0.15 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 6 0.15 No Hit CCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCT 6 0.15 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 6 0.15 No Hit GGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTG 6 0.15 No Hit GCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCAC 6 0.15 No Hit GGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGGCGTCTC 6 0.15 No Hit GTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTTCATGGGCCGC 6 0.15 No Hit GTTATGAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTTCAT 6 0.15 No Hit GTCCCGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCG 6 0.15 No Hit CCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTT 6 0.15 No Hit GCGGTTATGAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTT 6 0.15 No Hit GCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGA 5 0.125 No Hit CCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCA 5 0.125 No Hit CTTTTATCTAATAAATGCGCCCCTCCCAGAAGTCGGGGTTTGTTGCACGT 5 0.125 No Hit CCTTGTCCGTACCAGTTCTGAGTCGACTGTTCAGCGCTCGGGGAAAGCCC 5 0.125 No Hit CTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGC 5 0.125 No Hit GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC 5 0.125 No Hit GCCGACTTCCCTTGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTT 5 0.125 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 5 0.125 No Hit GTGCCGTTCACATGGAACCTTTCTCCTCTTCGGCCTTCAAAGTTCTCATT 5 0.125 No Hit AGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAG 5 0.125 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 5 0.125 No Hit CCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC 5 0.125 No Hit GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.075 0.0 0.0 0.0 0.0 9 0.075 0.0 0.0 0.0 0.0 10-11 0.075 0.0 0.0 0.0 0.0 12-13 0.075 0.0 0.0 0.0 0.0 14-15 0.075 0.0 0.0 0.0 0.0 16-17 0.075 0.0 0.0 0.0 0.0 18-19 0.075 0.0 0.0 0.0 0.0 20-21 0.075 0.0 0.0 0.0 0.0 22-23 0.075 0.0 0.0 0.0 0.0 24-25 0.075 0.0 0.0 0.0 0.0 26-27 0.075 0.0 0.0 0.0 0.0 28-29 0.075 0.0 0.0 0.0 0.0 30-31 0.125 0.0 0.0 0.0 0.0 32-33 0.15 0.0 0.0 0.0 0.0 34-35 0.15 0.0 0.0 0.0 0.0 36-37 0.15 0.0 0.0 0.0 0.0 38-39 0.175 0.0 0.0 0.0 0.0 40-41 0.2625 0.0 0.0 0.0 0.0 42-43 0.35 0.0 0.0 0.0 0.0 44-45 0.3875 0.0 0.0 0.0 0.0 46-47 0.4875 0.0 0.0 0.0 0.0 48-49 0.525 0.0 0.0 0.0 0.0 50-51 0.5875 0.0 0.0 0.0 0.0 52-53 0.6875 0.0 0.0 0.0 0.0 54-55 0.8125 0.0 0.0 0.0 0.0 56-57 1.0625 0.0 0.0 0.0 0.0 58-59 1.3125 0.0 0.0 0.0 0.0 60-61 1.6 0.0 0.0 0.0 0.0 62-63 1.9625 0.0 0.0 0.0 0.0 64-65 2.2249999999999996 0.0 0.0 0.0 0.0 66-67 2.6875 0.0 0.0 0.0 0.0 68-69 2.9875 0.0 0.0 0.0 0.0 70-71 3.3 0.0 0.0 0.0 0.0 72-73 3.6625 0.0 0.0 0.0 0.0 74-75 4.1125 0.0 0.0 0.0 0.0 76-77 4.5 0.0 0.0 0.0 0.0 78-79 4.9875 0.0 0.0 0.0 0.0 80-81 5.6875 0.0 0.0 0.0 0.0 82-83 6.2875 0.0 0.0 0.0 0.0 84-85 6.8125 0.0 0.0 0.0 0.0 86-87 7.4 0.0 0.0 0.0 0.0 88-89 8.075 0.0 0.0 0.0 0.0 90-91 8.5375 0.0 0.0 0.0 0.0 92-93 9.2625 0.0 0.0 0.0 0.0 94-95 9.9875 0.0 0.0 0.0 0.0 96-97 10.975000000000001 0.0 0.0 0.0 0.0 98-99 12.1125 0.0 0.0 0.0 0.0 100-101 13.025 0.0 0.0 0.0 0.0 102-103 14.287500000000001 0.0 0.0 0.0 0.0 104-105 15.2625 0.0 0.0 0.0 0.0 106-107 16.4375 0.0 0.0 0.0 0.0 108-109 17.3625 0.0 0.0 0.0 0.0 110-111 18.325 0.0 0.0 0.0 0.0 112-113 19.175 0.0 0.0 0.0 0.0 114-115 20.225 0.0 0.0 0.0 0.0 116-117 21.3625 0.0 0.0 0.0 0.0 118-119 22.45 0.0 0.0 0.0 0.0 120-121 23.325 0.0 0.0 0.0 0.0 122-123 24.3625 0.0 0.0 0.0 0.0 124-125 25.1625 0.0 0.0 0.0 0.0 126-127 26.1875 0.0 0.0 0.0 0.0 128-129 27.3 0.0 0.0 0.0 0.0 130-131 28.35 0.0 0.0 0.0 0.0 132-133 29.4625 0.0 0.0 0.0 0.0 134-135 30.575 0.0 0.0 0.0 0.0 136-137 31.7125 0.0 0.0 0.0 0.0 138 32.425 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAAATT 30 0.0018473949 72.0 8 GGGGGGG 575 2.1484651E-5 5.0086956 135-139 >>END_MODULE SRR24040056 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR24040056_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.388 37.0 37.0 37.0 37.0 37.0 2 35.9625 37.0 37.0 37.0 37.0 37.0 3 33.192 37.0 37.0 37.0 11.0 37.0 4 35.701 37.0 37.0 37.0 37.0 37.0 5 36.172 37.0 37.0 37.0 37.0 37.0 6 36.1025 37.0 37.0 37.0 37.0 37.0 7 36.2895 37.0 37.0 37.0 37.0 37.0 8 35.7075 37.0 37.0 37.0 37.0 37.0 9 34.6385 37.0 37.0 37.0 25.0 37.0 10-14 35.3091 37.0 37.0 37.0 34.6 37.0 15-19 35.26129999999999 37.0 37.0 37.0 32.2 37.0 20-24 35.6058 37.0 37.0 37.0 34.6 37.0 25-29 35.643699999999995 37.0 37.0 37.0 34.6 37.0 30-34 34.6046 37.0 37.0 37.0 29.4 37.0 35-39 35.767599999999995 37.0 37.0 37.0 37.0 37.0 40-44 35.6802 37.0 37.0 37.0 37.0 37.0 45-49 35.8238 37.0 37.0 37.0 37.0 37.0 50-54 35.81529999999999 37.0 37.0 37.0 37.0 37.0 55-59 35.822 37.0 37.0 37.0 37.0 37.0 60-64 35.7327 37.0 37.0 37.0 37.0 37.0 65-69 35.4948 37.0 37.0 37.0 34.6 37.0 70-74 35.4017 37.0 37.0 37.0 34.6 37.0 75-79 35.05980000000001 37.0 37.0 37.0 31.8 37.0 80-84 35.489799999999995 37.0 37.0 37.0 34.6 37.0 85-89 35.108799999999995 37.0 37.0 37.0 32.2 37.0 90-94 34.5114 37.0 37.0 37.0 27.0 37.0 95-99 35.2487 37.0 37.0 37.0 34.6 37.0 100-104 34.783100000000005 37.0 37.0 37.0 29.8 37.0 105-109 35.0531 37.0 37.0 37.0 32.2 37.0 110-114 34.39319999999999 37.0 37.0 37.0 22.2 37.0 115-119 34.467699999999994 37.0 37.0 37.0 25.0 37.0 120-124 34.038 37.0 37.0 37.0 25.0 37.0 125-129 33.988 37.0 37.0 37.0 25.0 37.0 130-134 33.659200000000006 37.0 37.0 37.0 19.4 37.0 135-139 33.5546 37.0 37.0 37.0 16.6 37.0 140-144 33.3775 37.0 37.0 37.0 11.0 37.0 145-149 33.140499999999996 37.0 37.0 37.0 11.0 37.0 150 33.2265 37.0 37.0 37.0 11.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 6.0 14 6.0 15 10.0 16 7.0 17 8.0 18 5.0 19 5.0 20 9.0 21 14.0 22 18.0 23 22.0 24 14.0 25 13.0 26 14.0 27 25.0 28 32.0 29 28.0 30 37.0 31 109.0 32 202.0 33 190.0 34 363.0 35 637.0 36 2043.0 37 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.2 19.275000000000002 6.550000000000001 28.975 2 35.575 21.275 23.375 19.775000000000002 3 28.525 23.75 23.375 24.349999999999998 4 30.55 29.925 17.5 22.025 5 31.825 31.4 16.075 20.7 6 29.849999999999998 32.275 16.025 21.85 7 27.3 18.875 28.225 25.6 8 28.749999999999996 20.45 21.975 28.825 9 28.575 21.15 24.5 25.775 10-14 29.915000000000003 24.725 19.505 25.855 15-19 30.380000000000003 23.325000000000003 20.61 25.685000000000002 20-24 30.475 24.01 20.59 24.925 25-29 29.794999999999998 24.560000000000002 19.855 25.790000000000003 30-34 29.375 24.595 20.64 25.39 35-39 29.304999999999996 25.395 19.950000000000003 25.35 40-44 29.925 23.810000000000002 20.495 25.77 45-49 29.7 24.525 20.23 25.545 50-54 29.599999999999998 25.2 20.085 25.115 55-59 29.815 24.115000000000002 21.349999999999998 24.72 60-64 30.795 24.12 20.345 24.740000000000002 65-69 29.815 24.490000000000002 20.205000000000002 25.490000000000002 70-74 30.925000000000004 23.76 19.950000000000003 25.365 75-79 31.540000000000003 23.25 20.315 24.895 80-84 31.480000000000004 24.154999999999998 19.77 24.595 85-89 31.264999999999997 24.19 19.509999999999998 25.035 90-94 31.655 24.855 19.63 23.86 95-99 32.75 24.23 19.46 23.56 100-104 32.769999999999996 25.080000000000002 19.605 22.545 105-109 34.13 24.169999999999998 19.735 21.965 110-114 34.56 24.615000000000002 18.615000000000002 22.21 115-119 35.49 23.225 19.145 22.14 120-124 36.265 23.62 18.755 21.36 125-129 37.765 23.549999999999997 18.495 20.19 130-134 38.655 23.095 18.060000000000002 20.19 135-139 40.985 22.66 17.285 19.07 140-144 42.61 22.42 16.55 18.42 145-149 44.82 21.13 15.870000000000001 18.18 150 44.824999999999996 20.724999999999998 17.075000000000003 17.375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 1.0 4 1.0 5 0.0 6 0.0 7 1.5 8 2.0 9 1.0 10 1.0 11 0.5 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 1.0 18 2.0 19 2.0 20 2.0 21 1.5 22 0.5 23 1.0 24 2.0 25 2.0 26 2.0 27 3.0 28 3.5 29 2.0 30 1.5 31 3.0 32 4.5 33 3.5 34 5.0 35 10.5 36 14.0 37 18.5 38 21.5 39 25.5 40 36.0 41 45.0 42 47.0 43 60.5 44 80.0 45 90.5 46 106.5 47 142.5 48 156.5 49 176.0 50 182.0 51 170.0 52 165.5 53 161.5 54 166.5 55 155.5 56 140.5 57 127.0 58 130.5 59 124.0 60 111.0 61 117.5 62 117.0 63 102.5 64 97.0 65 86.5 66 80.5 67 94.5 68 97.0 69 77.0 70 64.5 71 66.0 72 56.0 73 45.0 74 34.5 75 26.0 76 25.0 77 18.5 78 15.5 79 9.0 80 2.0 81 4.0 82 3.0 83 1.0 84 1.0 85 0.0 86 0.5 87 1.0 88 0.5 89 1.0 90 1.0 91 2.0 92 2.5 93 1.5 94 1.5 95 1.0 96 4.0 97 5.5 98 4.0 99 6.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.075 #Duplication Level Percentage of deduplicated Percentage of total 1 86.12395929694728 69.825 2 9.096515572001234 14.75 3 2.682701202590194 6.525 4 1.0484119642306506 3.4000000000000004 5 0.46253469010175763 1.875 6 0.27752081406105455 1.35 7 0.12334258402713535 0.7000000000000001 8 0.03083564600678384 0.2 9 0.03083564600678384 0.22499999999999998 >10 0.12334258402713535 1.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 13 0.325 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 12 0.3 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 11 0.27499999999999997 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 10 0.25 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 9 0.22499999999999998 No Hit CAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTC 8 0.2 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 7 0.17500000000000002 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 7 0.17500000000000002 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 7 0.17500000000000002 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 7 0.17500000000000002 No Hit CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA 6 0.15 No Hit CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTA 6 0.15 No Hit CGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCC 6 0.15 No Hit GTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCA 6 0.15 No Hit GCCAGAGGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCG 6 0.15 No Hit GTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGA 6 0.15 No Hit AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA 6 0.15 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 6 0.15 No Hit GTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCT 6 0.15 No Hit GGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAAC 5 0.125 No Hit GTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGATGGTGAACTATGCC 5 0.125 No Hit GGACGGGGTAACCCCGGCAGATAGCGCGATCACGCGTATCCCCCGAAAGG 5 0.125 No Hit AGCGCGATCACGCGTATCCCCCGAAAGGGAATCGGGTTAAGATTTCCCGA 5 0.125 No Hit TACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGA 5 0.125 No Hit CGAAAGATGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTG 5 0.125 No Hit CTTCAGCCGGCGATGCGCTCCTAGCCTTAATTGGCCGGGTCGTGCCTCCG 5 0.125 No Hit GTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGA 5 0.125 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 5 0.125 No Hit GCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATT 5 0.125 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 5 0.125 No Hit AGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAA 5 0.125 No Hit GTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 5 0.125 No Hit GGGTAACCCCGGCAGATAGCGCGATCACGCGTATCCCCCGAAAGGGAATC 5 0.125 No Hit CCCAAATCGGGCGGTAGACTCCGTCCAAGGCTAAATACAGGCGAGAGACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.075 0.0 0.0 0.0 0.0 9 0.075 0.0 0.0 0.0 0.0 10-11 0.075 0.0 0.0 0.0 0.0 12-13 0.075 0.0 0.0 0.0 0.0 14-15 0.075 0.0 0.0 0.0 0.0 16-17 0.075 0.0 0.0 0.0 0.0 18-19 0.075 0.0 0.0 0.0 0.0 20-21 0.075 0.0 0.0 0.0 0.0 22-23 0.075 0.0 0.0 0.0 0.0 24-25 0.075 0.0 0.0 0.0 0.0 26-27 0.075 0.0 0.0 0.0 0.0 28-29 0.075 0.0 0.0 0.0 0.0 30-31 0.125 0.0 0.0 0.0 0.0 32-33 0.15 0.0 0.0 0.0 0.0 34-35 0.15 0.0 0.0 0.0 0.0 36-37 0.15 0.0 0.0 0.0 0.0 38-39 0.175 0.0 0.0 0.0 0.0 40-41 0.2625 0.0 0.0 0.0 0.0 42-43 0.35 0.0 0.0 0.0 0.0 44-45 0.3875 0.0 0.0 0.0 0.0 46-47 0.5125 0.0 0.0 0.0 0.0 48-49 0.55 0.0 0.0 0.0 0.0 50-51 0.6125 0.0 0.0 0.0 0.0 52-53 0.7250000000000001 0.0 0.0 0.0 0.0 54-55 0.8625 0.0 0.0 0.0 0.0 56-57 1.1124999999999998 0.0 0.0 0.0 0.0 58-59 1.3625 0.0 0.0 0.0 0.0 60-61 1.675 0.0 0.0 0.0 0.0 62-63 2.0375 0.0 0.0 0.0 0.0 64-65 2.3375 0.0 0.0 0.0 0.0 66-67 2.775 0.0 0.0 0.0 0.0 68-69 3.0625 0.0 0.0 0.0 0.0 70-71 3.375 0.0 0.0 0.0 0.0 72-73 3.75 0.0 0.0 0.0 0.0 74-75 4.199999999999999 0.0 0.0 0.0 0.0 76-77 4.574999999999999 0.0 0.0 0.0 0.0 78-79 5.025 0.0 0.0 0.0 0.0 80-81 5.6875 0.0 0.0 0.0 0.0 82-83 6.275 0.0 0.0 0.0 0.0 84-85 6.7875 0.0 0.0 0.0 0.0 86-87 7.3375 0.0 0.0 0.0 0.0 88-89 7.975 0.0 0.0 0.0 0.0 90-91 8.4375 0.0 0.0 0.0 0.0 92-93 9.1625 0.0 0.0 0.0 0.0 94-95 9.8875 0.0 0.0 0.0 0.0 96-97 10.9125 0.0 0.0 0.0 0.0 98-99 12.025 0.0 0.0 0.0 0.0 100-101 12.925 0.0 0.0 0.0 0.0 102-103 14.212499999999999 0.0 0.0 0.0 0.0 104-105 15.1625 0.0 0.0 0.0 0.0 106-107 16.2625 0.0 0.0 0.0 0.0 108-109 17.15 0.0 0.0 0.0 0.0 110-111 18.1 0.0 0.0 0.0 0.0 112-113 18.975 0.0 0.0 0.0 0.0 114-115 20.025 0.0 0.0 0.0 0.0 116-117 21.15 0.0 0.0 0.0 0.0 118-119 22.175 0.0 0.0 0.0 0.0 120-121 23.0875 0.0 0.0 0.0 0.0 122-123 24.125 0.0 0.0 0.0 0.0 124-125 24.9125 0.0 0.0 0.0 0.0 126-127 25.9125 0.0 0.0 0.0 0.0 128-129 27.05 0.0 0.0 0.0 0.0 130-131 28.1 0.0 0.0 0.0 0.0 132-133 29.2375 0.0 0.0 0.0 0.0 134-135 30.3375 0.0 0.0 0.0 0.0 136-137 31.450000000000003 0.0 0.0 0.0 0.0 138 32.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACCAC 10 0.006973645 144.0 9 AGAAAAG 10 0.006973645 144.0 2 AAAGTTA 10 0.006973645 144.0 5 CAGAAAA 10 0.006973645 144.0 1 GTTACCA 10 0.006973645 144.0 8 AAGTTAC 10 0.006973645 144.0 6 AGTTACC 10 0.006973645 144.0 7 GGGGGGG 1645 0.0 5.0772038 140-144 >>END_MODULE Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616928 spots for SRR24040056.sra Written 3616928 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra Read 3616926 spots for SRR24040056.sra Written 3616926 spots for SRR24040056.sra SRR ids: ['SRR24040056.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4vjbz19p SRR24040056.sra spots: 72338522 blocks: [[1, 3616926], [3616927, 7233852], [7233853, 10850778], [10850779, 14467704], [14467705, 18084630], [18084631, 21701556], [21701557, 25318482], [25318483, 28935408], [28935409, 32552334], [32552335, 36169260], [36169261, 39786186], [39786187, 43403112], [43403113, 47020038], [47020039, 50636964], [50636965, 54253890], [54253891, 57870816], [57870817, 61487742], [61487743, 65104668], [65104669, 68721594], [68721595, 72338522]] SRR24040056 file size 24420808 SRR24040056 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR24040056 SRR24040056_1.fastq SRR24040056_2.fastq Input file: SRR24040056_1.fastq Paired file: SRR24040056_2.fastq trimmed: SRR24040056-trimmed-pair1.fastq, SRR24040056-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 22:08:35 2024 >> started Fri Dec 6 22:09:55 2024 >> done (79.489s) 72338522 read pairs processed; of these: 17746 ( 0.02%) short read pairs filtered out after trimming by size control 552326 ( 0.76%) empty read pairs filtered out after trimming by size control 71768450 (99.21%) read pairs available; of these: 28096392 (39.15%) trimmed read pairs available after processing 43672058 (60.85%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 664 0.00% 19 695 0.00% 20 438 0.00% 21 615 0.00% 22 659 0.00% 23 489 0.00% 24 646 0.00% 25 845 0.00% 26 950 0.00% 27 1345 0.00% 28 1924 0.00% 29 2259 0.00% 30 3821 0.01% 31 3157 0.00% 32 3381 0.00% 33 4217 0.01% 34 4527 0.01% 35 5015 0.01% 36 5936 0.01% 37 6926 0.01% 38 8593 0.01% 39 10239 0.01% 40 12258 0.02% 41 13838 0.02% 42 14705 0.02% 43 15981 0.02% 44 17664 0.02% 45 19752 0.03% 46 22332 0.03% 47 25469 0.04% 48 31662 0.04% 49 35353 0.05% 50 40070 0.06% 51 44297 0.06% 52 47906 0.07% 53 50668 0.07% 54 52510 0.07% 55 53083 0.07% 56 59000 0.08% 57 64606 0.09% 58 75304 0.10% 59 82046 0.11% 60 89379 0.12% 61 97680 0.14% 62 107509 0.15% 63 107858 0.15% 64 109282 0.15% 65 109465 0.15% 66 114643 0.16% 67 121054 0.17% 68 127263 0.18% 69 134166 0.19% 70 137859 0.19% 71 150862 0.21% 72 166761 0.23% 73 179452 0.25% 74 178555 0.25% 75 178197 0.25% 76 182554 0.25% 77 184423 0.26% 78 190989 0.27% 79 204076 0.28% 80 221408 0.31% 81 229766 0.32% 82 237342 0.33% 83 241249 0.34% 84 250709 0.35% 85 260594 0.36% 86 276220 0.38% 87 278781 0.39% 88 270690 0.38% 89 277830 0.39% 90 281964 0.39% 91 282275 0.39% 92 307961 0.43% 93 328524 0.46% 94 316948 0.44% 95 338671 0.47% 96 337024 0.47% 97 339104 0.47% 98 353287 0.49% 99 342490 0.48% 100 359761 0.50% 101 346527 0.48% 102 353208 0.49% 103 367887 0.51% 104 355908 0.50% 105 343118 0.48% 106 350524 0.49% 107 349648 0.49% 108 337410 0.47% 109 345907 0.48% 110 336234 0.47% 111 357933 0.50% 112 361718 0.50% 113 352927 0.49% 114 368446 0.51% 115 376281 0.52% 116 380524 0.53% 117 358133 0.50% 118 347058 0.48% 119 364600 0.51% 120 373235 0.52% 121 367067 0.51% 122 378905 0.53% 123 388262 0.54% 124 396119 0.55% 125 394557 0.55% 126 395500 0.55% 127 384342 0.54% 128 371024 0.52% 129 389062 0.54% 130 361751 0.50% 131 367323 0.51% 132 369817 0.52% 133 387307 0.54% 134 388903 0.54% 135 385226 0.54% 136 388907 0.54% 137 389767 0.54% 138 383771 0.53% 139 380639 0.53% 140 371641 0.52% 141 375375 0.52% 142 390242 0.54% 143 380845 0.53% 144 405283 0.56% 145 386029 0.54% 146 391941 0.55% 147 394336 0.55% 148 378288 0.53% 149 376467 0.52% 150 43672058 60.85% 71768450 reads passed initial QC criterion=sequence-density sequence-density=1.03 sequence-density-rank=1 fanout-score=2.81 fanout-score-rank=24 prefix-density=1.07 prefix-fanout=2.7 sequence=CCCAGCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGATCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTTGCCCCGCTTCCGACCCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC criterion=fanout-score sequence-density=0.78 sequence-density-rank=2 fanout-score=35.53 fanout-score-rank=1 prefix-density=0.79 prefix-fanout=34.9 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAGGGGG criterion=sequence-density sequence-density=0.80 sequence-density-rank=1 fanout-score=2.38 fanout-score-rank=25 prefix-density=0.80 prefix-fanout=2.4 sequence=TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGTGCCGGATTATGACTGAACGCCTCTAAGTCAGAATCCAAGCTAGCAAGCGGCGCCTGCGCCCGCCGCCTGCCCCGACCCACGTTAGGGGCGCTTGCGCCCCCAAGGGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCGCCTCGAAGCTCCCTTCCCCACGGGCGGCGGGCTGAATCCTTTGCAGACGACTTAAATACGCGACGGGGCATTGTAAGTGGCAGAGTGGCCTTGCTGCCACGATCCACTGAGATCCAGCCCCACGTCGCACGGAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=257.63 fanout-score-rank=1 prefix-density=0.66 prefix-fanout=5.1 sequence=GCAGCAGCAAGAACAGGGGCAGCAAGGTCAGATCCCTTCGCGCCGGTTCGGGGCCCAGTCACAGGAGTGCGGACAACAGGGTATCTTCTCGCCCCAGCTGCTAGCTCAGATACTACCGGCGATGTGCAATGTGTACCTCCCACCGTACTGCCGCGCCACCGCCGCTGATCAGCCATTCGGACCCGGTGGCTACTGATAAGACAAAGGGCTCTTGTAGTAGATAGATAGGGATCACCGCT SRR24040056 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 22:10:50 Started mapping on | Dec 06 22:10:51 Finished on | Dec 06 22:19:52 Mapping speed, Million of reads per hour | 477.57 Number of input reads | 71768450 Average input read length | 268 UNIQUE READS: Uniquely mapped reads number | 26064195 Uniquely mapped reads % | 36.32% Average mapped length | 270.95 Number of splices: Total | 16563161 Number of splices: Annotated (sjdb) | 15522653 Number of splices: GT/AG | 16218960 Number of splices: GC/AG | 207943 Number of splices: AT/AC | 8300 Number of splices: Non-canonical | 127958 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.01% Deletion average length | 2.14 Insertion rate per base | 0.01% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 3992233 % of reads mapped to multiple loci | 5.56% Number of reads mapped to too many loci | 7073100 % of reads mapped to too many loci | 9.86% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.95% % of reads unmapped: other | 44.31% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 41712022 41712022 41712022 N_multimapping 3992233 3992233 3992233 N_noFeature 1477781 25500130 1735493 N_ambiguous 438501 1590 176940 UnstrandedReadsAssigned:24147913 PositiveStrandReadsAssigned:562475 NegativeStrandReadsAssigned:24151762 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=112 echo kmer=107 SRR24040056 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR24040056-trimmed-pair1.fastq SRR24040056-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 71,768,450 reads, 27,035,961 reads pseudoaligned [quant] estimated average fragment length: 181.64 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,153 rounds 52973 SRR24040056.ke.tsv 35125 SRR24040056.se.tsv 88098 total ==> SRR24040056.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 755.557 108.928 6.05788 PNS24247 1044 863.36 25.5795 1.24494 PNS24249 1928 1747.36 202.944 4.88028 PNS24246 1044 863.36 25.5795 1.24494 PNS24248 1044 863.36 25.5795 1.24494 PNS24244 1471 1290.36 46.3896 1.51064 PNS24243 293 130.953 0 0 KQK14069 1603 1422.36 5458.96 161.269 KQK14071 474 295.711 19.8427 2.81957 ==> SRR24040056.se.tsv <== BRADI_1g14170v3 5359 BRADI_1g53295v3 26 BRADI_1g59795v3 26 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 368 BRADI_1g74790v3 44 BRADI_1g09890v3 9 BRADI_1g77505v3 207 BRADI_1g48960v3 5 SRR24040056 completed mapping pipeline successfully