Starting /dee2/code/volunteer_pipeline.sh SRR24040058 current disk space = 1548824125440 free memory = 1389686232 SRR24040058 SRAfilesize 53c174a282ae7ab6b4d7845ccf12e570 SRR24040058.sra SRR24040058.sra file validated SRR24040058 is paired end SRR24040058 is conventional basespace SRR24040058 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR24040058_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.78775 37.0 37.0 37.0 37.0 37.0 2 36.448 37.0 37.0 37.0 37.0 37.0 3 36.3255 37.0 37.0 37.0 37.0 37.0 4 36.675 37.0 37.0 37.0 37.0 37.0 5 36.607 37.0 37.0 37.0 37.0 37.0 6 35.0525 37.0 37.0 37.0 25.0 37.0 7 36.6765 37.0 37.0 37.0 37.0 37.0 8 36.6015 37.0 37.0 37.0 37.0 37.0 9 32.7435 37.0 37.0 37.0 11.0 37.0 10-14 36.565 37.0 37.0 37.0 37.0 37.0 15-19 36.4534 37.0 37.0 37.0 37.0 37.0 20-24 36.4269 37.0 37.0 37.0 37.0 37.0 25-29 36.4478 37.0 37.0 37.0 37.0 37.0 30-34 36.096900000000005 37.0 37.0 37.0 34.6 37.0 35-39 36.4943 37.0 37.0 37.0 37.0 37.0 40-44 36.3541 37.0 37.0 37.0 37.0 37.0 45-49 36.2816 37.0 37.0 37.0 37.0 37.0 50-54 36.4073 37.0 37.0 37.0 37.0 37.0 55-59 36.1899 37.0 37.0 37.0 37.0 37.0 60-64 36.2691 37.0 37.0 37.0 37.0 37.0 65-69 36.3977 37.0 37.0 37.0 37.0 37.0 70-74 36.391 37.0 37.0 37.0 37.0 37.0 75-79 36.392500000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.9948 37.0 37.0 37.0 37.0 37.0 85-89 36.292500000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.33815 37.0 37.0 37.0 37.0 37.0 95-99 36.2997 37.0 37.0 37.0 37.0 37.0 100-104 36.2365 37.0 37.0 37.0 37.0 37.0 105-109 36.3418 37.0 37.0 37.0 37.0 37.0 110-114 36.1978 37.0 37.0 37.0 37.0 37.0 115-119 35.91235 37.0 37.0 37.0 34.6 37.0 120-124 35.85754999999999 37.0 37.0 37.0 37.0 37.0 125-129 36.0946 37.0 37.0 37.0 37.0 37.0 130-134 35.7626 37.0 37.0 37.0 34.6 37.0 135-139 35.91425 37.0 37.0 37.0 37.0 37.0 140-144 35.592349999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.462450000000004 37.0 37.0 37.0 34.6 37.0 150 32.1965 37.0 37.0 37.0 11.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 1.0 24 1.0 25 6.0 26 10.0 27 10.0 28 11.0 29 14.0 30 33.0 31 26.0 32 48.0 33 68.0 34 165.0 35 415.0 36 2850.0 37 341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.729662077597 9.687108886107634 4.305381727158949 42.27784730913642 2 24.175 10.6 34.75 30.475 3 21.625 13.55 23.974999999999998 40.849999999999994 4 30.225 21.5 20.1 28.175 5 28.075 25.45 22.475 24.0 6 24.474999999999998 27.750000000000004 24.825 22.95 7 19.225 19.35 40.675 20.75 8 20.5 17.775 32.800000000000004 28.925 9 20.5 20.3 31.25 27.950000000000003 10-14 25.135 23.69 24.11 27.065 15-19 25.35 22.0 24.29 28.360000000000003 20-24 25.5 23.1 24.66 26.740000000000002 25-29 24.8 22.264999999999997 24.33 28.605000000000004 30-34 25.040000000000003 21.015 24.985 28.96 35-39 24.759999999999998 21.705 24.759999999999998 28.775000000000002 40-44 25.430000000000003 21.555 24.884999999999998 28.13 45-49 24.474999999999998 21.605 25.6 28.32 50-54 24.055 22.715 24.965 28.265 55-59 23.985 21.765 25.509999999999998 28.74 60-64 24.07 20.82 26.71 28.4 65-69 23.425 22.67 25.474999999999998 28.43 70-74 25.674999999999997 22.235 23.93 28.16 75-79 25.724999999999998 22.14 23.97 28.165000000000003 80-84 25.14 21.705 24.81 28.345 85-89 25.46 21.66 24.4 28.48 90-94 25.2437865679852 21.84327649147372 24.638695804370656 28.274241136170424 95-99 23.865 22.91 24.37 28.854999999999997 100-104 25.215 22.99 23.395 28.4 105-109 24.53745374537454 23.697369736973698 24.302430243024304 27.462746274627463 110-114 25.83 23.615 23.24 27.315 115-119 26.1039155873381 23.04845726859029 22.91843776566485 27.92918937840676 120-124 26.14153538384596 23.135783945986496 23.415853963490875 27.306826706676667 125-129 25.765 22.46 22.775000000000002 28.999999999999996 130-134 26.27 22.7 23.485 27.544999999999998 135-139 25.873881082162324 23.368505275791367 23.363504525678852 27.394109116367453 140-144 25.663849577436615 23.403510526578984 23.268490273541033 27.664149622443368 145-149 25.923888583287493 21.898284742711407 24.028604290643596 28.149222383357504 150 25.324999999999996 25.174999999999997 23.625 25.874999999999996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.0 25 0.0 26 0.0 27 1.0 28 2.0 29 2.0 30 1.0 31 1.0 32 2.5 33 4.5 34 5.0 35 7.5 36 16.0 37 19.5 38 18.5 39 27.0 40 35.0 41 48.5 42 71.0 43 87.5 44 107.5 45 122.0 46 144.5 47 177.5 48 182.0 49 206.0 50 223.0 51 226.5 52 202.0 53 164.0 54 151.5 55 150.5 56 174.5 57 155.0 58 126.0 59 119.0 60 127.0 61 122.5 62 107.0 63 110.5 64 99.5 65 81.5 66 69.5 67 62.5 68 51.0 69 31.5 70 31.5 71 38.5 72 28.5 73 17.5 74 15.0 75 9.5 76 6.5 77 5.5 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.015 95-99 0.0 100-104 0.0 105-109 0.01 110-114 0.0 115-119 0.015 120-124 0.025 125-129 0.0 130-134 0.0 135-139 0.015 140-144 0.015 145-149 0.015 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.39999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 81.73342541436463 59.175 2 10.082872928176796 14.6 3 3.9019337016574585 8.475000000000001 4 1.5883977900552488 4.6 5 1.0359116022099446 3.75 6 0.6560773480662984 2.85 7 0.3798342541436464 1.925 8 0.24171270718232044 1.4000000000000001 9 0.13812154696132595 0.8999999999999999 >10 0.24171270718232044 2.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 22 0.5499999999999999 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 13 0.325 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 13 0.325 No Hit GGGAAATTCTGACCGTTGAGGCGTGCGATACTGCCAGCACGTGGGTTGTA 12 0.3 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 12 0.3 No Hit CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC 11 0.27499999999999997 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 10 0.25 No Hit GTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC 9 0.22499999999999998 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 9 0.22499999999999998 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 9 0.22499999999999998 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 9 0.22499999999999998 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 8 0.2 No Hit CCCCCATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTT 8 0.2 No Hit GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG 8 0.2 No Hit CATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAAT 8 0.2 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 8 0.2 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 8 0.2 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 8 0.2 No Hit CCCGATTCCATGGCGCGGCTCACCGGAGCAGCCGCGCCGTCCTACCTATT 7 0.17500000000000002 No Hit ATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAA 7 0.17500000000000002 No Hit CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC 7 0.17500000000000002 No Hit CCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTAT 7 0.17500000000000002 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 7 0.17500000000000002 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 7 0.17500000000000002 No Hit CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT 7 0.17500000000000002 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 7 0.17500000000000002 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 7 0.17500000000000002 No Hit CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAGGGAT 7 0.17500000000000002 No Hit GGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCAC 7 0.17500000000000002 No Hit CCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCA 6 0.15 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 6 0.15 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 6 0.15 No Hit GTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAG 6 0.15 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 6 0.15 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 6 0.15 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC 6 0.15 No Hit GTCAGTATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGC 6 0.15 No Hit GTTATTTTTAAGGTTTTGAGCTTCTTGCCTAGAGATGCGGTACGCATTGG 6 0.15 No Hit GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC 6 0.15 No Hit GTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGC 6 0.15 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 6 0.15 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 6 0.15 No Hit CAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGA 6 0.15 No Hit CTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCT 6 0.15 No Hit CCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGT 6 0.15 No Hit CGTTGGTCTTGAAAGCGATATACTGGTATCCTTCACTCTCCGCCTTCTTT 6 0.15 No Hit GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT 6 0.15 No Hit CCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTT 6 0.15 No Hit CTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGG 5 0.125 No Hit GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTA 5 0.125 No Hit GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGT 5 0.125 No Hit ATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGC 5 0.125 No Hit CGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTC 5 0.125 No Hit GTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATC 5 0.125 No Hit GGTAGCTTCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGT 5 0.125 No Hit GCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCC 5 0.125 No Hit CCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTA 5 0.125 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 5 0.125 No Hit GTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCC 5 0.125 No Hit CAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC 5 0.125 No Hit GCCGGGACCCCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCCGAAGTTA 5 0.125 No Hit GCCCACTTGGAGCTCCCGATTCCATGGCGCGGCTCACCGGAGCAGCCGCG 5 0.125 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 5 0.125 No Hit GCCGACTTCCCTTGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTT 5 0.125 No Hit CCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCC 5 0.125 No Hit GGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCGCGCGCTTTAGCG 5 0.125 No Hit GGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCG 5 0.125 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 5 0.125 No Hit CCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCA 5 0.125 No Hit CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 5 0.125 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 5 0.125 No Hit CGGTTATCCGAGTAGCACGTACCATCAAACAAACTATAACTGATTTAATG 5 0.125 No Hit GTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAG 5 0.125 No Hit CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATT 5 0.125 No Hit CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCCCGA 5 0.125 No Hit CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCACGA 5 0.125 No Hit CCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGAC 5 0.125 No Hit GGCACGGTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.025 0.0 7 0.0 0.0 0.0 0.025 0.0 8 0.0 0.0 0.0 0.025 0.0 9 0.0 0.0 0.0 0.025 0.0 10-11 0.025 0.0 0.0 0.025 0.0 12-13 0.025 0.0 0.0 0.025 0.0 14-15 0.025 0.0 0.0 0.025 0.0 16-17 0.025 0.0 0.0 0.025 0.0 18-19 0.025 0.0 0.0 0.025 0.0 20-21 0.025 0.0 0.0 0.025 0.0 22-23 0.025 0.0 0.0 0.025 0.0 24-25 0.025 0.0 0.0 0.025 0.0 26-27 0.025 0.0 0.0 0.025 0.0 28-29 0.025 0.0 0.0 0.025 0.0 30-31 0.05 0.0 0.0 0.025 0.0 32-33 0.075 0.0 0.0 0.025 0.0 34-35 0.075 0.0 0.0 0.025 0.0 36-37 0.075 0.0 0.0 0.025 0.0 38-39 0.075 0.0 0.0 0.025 0.0 40-41 0.075 0.0 0.0 0.025 0.0 42-43 0.075 0.0 0.0 0.025 0.0 44-45 0.1125 0.0 0.0 0.025 0.0 46-47 0.15 0.0 0.0 0.025 0.0 48-49 0.225 0.0 0.0 0.025 0.0 50-51 0.2375 0.0 0.0 0.025 0.0 52-53 0.2625 0.0 0.0 0.025 0.0 54-55 0.3 0.0 0.0 0.025 0.0 56-57 0.3375 0.0 0.0 0.025 0.0 58-59 0.3625 0.0 0.0 0.025 0.0 60-61 0.4125 0.0 0.0 0.025 0.0 62-63 0.475 0.0 0.0 0.025 0.0 64-65 0.5375000000000001 0.0 0.0 0.025 0.0 66-67 0.5625 0.0 0.0 0.025 0.0 68-69 0.6125 0.0 0.0 0.025 0.0 70-71 0.775 0.0 0.0 0.025 0.0 72-73 0.9875 0.0 0.0 0.025 0.0 74-75 1.175 0.0 0.0 0.025 0.0 76-77 1.375 0.0 0.0 0.025 0.0 78-79 1.5 0.0 0.0 0.025 0.0 80-81 1.625 0.0 0.0 0.025 0.0 82-83 1.875 0.0 0.0 0.025 0.0 84-85 2.25 0.0 0.0 0.025 0.0 86-87 2.5999999999999996 0.0 0.0 0.025 0.0 88-89 3.0 0.0 0.0 0.025 0.0 90-91 3.35 0.0 0.0 0.025 0.0 92-93 3.9 0.0 0.0 0.025 0.0 94-95 4.475 0.0 0.0 0.025 0.0 96-97 5.0 0.0 0.0 0.025 0.0 98-99 5.4625 0.0 0.0 0.025 0.0 100-101 6.05 0.0 0.0 0.025 0.0 102-103 6.7125 0.0 0.0 0.025 0.0 104-105 7.25 0.0 0.0 0.025 0.0 106-107 7.8125 0.0 0.0 0.025 0.0 108-109 8.45 0.0 0.0 0.025 0.0 110-111 9.25 0.0 0.0 0.025 0.0 112-113 9.975000000000001 0.0 0.0 0.025 0.0 114-115 10.7625 0.0 0.0 0.025 0.0 116-117 11.3875 0.0 0.0 0.025 0.0 118-119 12.2375 0.0 0.0 0.025 0.0 120-121 13.075 0.0 0.0 0.025 0.0 122-123 13.9375 0.0 0.0 0.025 0.0 124-125 14.8625 0.0 0.0 0.025 0.0 126-127 16.0875 0.0 0.0 0.025 0.0 128-129 17.112499999999997 0.0 0.0 0.025 0.0 130-131 18.0875 0.0 0.0 0.025 0.0 132-133 19.0625 0.0 0.0 0.025 0.0 134-135 19.8625 0.0 0.0 0.025 0.0 136-137 20.525 0.0 0.0 0.025 0.0 138 21.2 0.0 0.0 0.025 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCCAA 10 0.006973645 144.0 4 CCCAGCC 10 0.006973645 144.0 2 CCAGCCA 10 0.006973645 144.0 3 AATTAAC 10 0.006973645 144.0 6 TAACCAG 10 0.006973645 144.0 9 ATTAACC 10 0.006973645 144.0 7 GAATTAA 10 0.006973645 144.0 5 GCCAAAC 10 0.006973645 144.0 6 GGAATTA 10 0.006973645 144.0 4 CCCCAGC 10 0.006973645 144.0 1 >>END_MODULE SRR24040058 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR24040058_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4215 37.0 37.0 37.0 37.0 37.0 2 36.126 37.0 37.0 37.0 37.0 37.0 3 33.1965 37.0 37.0 37.0 11.0 37.0 4 35.9495 37.0 37.0 37.0 37.0 37.0 5 36.443 37.0 37.0 37.0 37.0 37.0 6 36.3215 37.0 37.0 37.0 37.0 37.0 7 36.3055 37.0 37.0 37.0 37.0 37.0 8 35.8565 37.0 37.0 37.0 37.0 37.0 9 34.82 37.0 37.0 37.0 25.0 37.0 10-14 35.80460000000001 37.0 37.0 37.0 37.0 37.0 15-19 35.5502 37.0 37.0 37.0 34.6 37.0 20-24 35.7247 37.0 37.0 37.0 34.6 37.0 25-29 35.917899999999996 37.0 37.0 37.0 37.0 37.0 30-34 34.7315 37.0 34.6 37.0 29.4 37.0 35-39 36.0576 37.0 37.0 37.0 37.0 37.0 40-44 36.0321 37.0 37.0 37.0 37.0 37.0 45-49 36.0907 37.0 37.0 37.0 37.0 37.0 50-54 36.1177 37.0 37.0 37.0 37.0 37.0 55-59 36.0943 37.0 37.0 37.0 37.0 37.0 60-64 36.0327 37.0 37.0 37.0 37.0 37.0 65-69 35.761199999999995 37.0 37.0 37.0 34.6 37.0 70-74 35.6639 37.0 37.0 37.0 34.6 37.0 75-79 35.1593 37.0 37.0 37.0 31.8 37.0 80-84 35.7159 37.0 37.0 37.0 34.6 37.0 85-89 35.410000000000004 37.0 37.0 37.0 32.2 37.0 90-94 34.9074 37.0 37.0 37.0 27.0 37.0 95-99 35.7422 37.0 37.0 37.0 37.0 37.0 100-104 35.306799999999996 37.0 37.0 37.0 32.2 37.0 105-109 35.6509 37.0 37.0 37.0 37.0 37.0 110-114 35.036699999999996 37.0 37.0 37.0 32.2 37.0 115-119 35.407799999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.0438 37.0 37.0 37.0 32.2 37.0 125-129 35.0015 37.0 37.0 37.0 29.8 37.0 130-134 34.794500000000006 37.0 37.0 37.0 25.0 37.0 135-139 34.634100000000004 37.0 37.0 37.0 25.0 37.0 140-144 34.49575 37.0 37.0 37.0 25.0 37.0 145-149 34.15735 37.0 37.0 37.0 25.0 37.0 150 34.1135 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 9.0 14 12.0 15 9.0 16 6.0 17 2.0 18 5.0 19 1.0 20 7.0 21 9.0 22 10.0 23 13.0 24 7.0 25 7.0 26 8.0 27 7.0 28 13.0 29 21.0 30 28.0 31 48.0 32 85.0 33 133.0 34 276.0 35 774.0 36 2325.0 37 184.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.2 19.900000000000002 6.6000000000000005 33.300000000000004 2 33.875 21.45 26.275 18.4 3 23.225 25.974999999999998 24.175 26.625 4 27.775 31.85 18.125 22.25 5 30.9 32.725 16.725 19.650000000000002 6 26.5 34.75 17.2 21.55 7 24.875 19.400000000000002 31.525 24.2 8 24.575 21.2 23.425 30.8 9 25.825 23.175 25.974999999999998 25.025 10-14 28.050000000000004 25.6 20.695 25.655 15-19 28.685 25.195 21.490000000000002 24.63 20-24 28.775000000000002 25.240000000000002 21.705 24.279999999999998 25-29 27.939999999999998 25.419999999999998 21.345 25.295 30-34 27.435 26.115 21.21 25.240000000000002 35-39 27.26 26.075 21.275 25.39 40-44 28.315 24.865000000000002 21.4 25.419999999999998 45-49 27.88 25.240000000000002 21.32 25.56 50-54 28.405 25.235000000000003 21.625 24.735 55-59 28.335 24.785 22.470000000000002 24.41 60-64 28.895 24.025 21.745 25.335 65-69 28.08 25.35 21.395 25.174999999999997 70-74 28.22 24.985 21.395 25.4 75-79 28.835 23.794999999999998 21.83 25.540000000000003 80-84 29.435 24.685000000000002 20.935000000000002 24.945 85-89 29.134999999999998 24.224999999999998 21.525 25.115 90-94 28.665000000000003 25.009999999999998 21.805 24.52 95-99 29.799999999999997 25.005 21.25 23.945 100-104 29.28 25.8 21.695 23.225 105-109 29.825000000000003 24.990000000000002 21.91 23.275000000000002 110-114 31.055 25.230000000000004 20.41 23.305 115-119 31.075000000000003 25.86 20.47 22.595000000000002 120-124 31.72 25.355 20.349999999999998 22.575 125-129 32.275 25.335 19.97 22.42 130-134 33.195 25.345000000000002 19.57 21.89 135-139 34.849999999999994 23.905 19.84 21.404999999999998 140-144 35.13527029054358 24.48367255088263 19.63794569185378 20.743111466720006 145-149 36.426821341067054 24.866243312165608 18.590929546477327 20.116005800290015 150 36.1 25.874999999999996 19.325 18.7 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 1.0 14 1.5 15 1.5 16 1.0 17 0.5 18 0.5 19 0.0 20 0.0 21 1.5 22 2.0 23 1.0 24 1.5 25 1.5 26 0.5 27 0.0 28 1.0 29 2.5 30 3.5 31 3.5 32 3.5 33 4.5 34 7.5 35 13.5 36 15.0 37 17.0 38 24.5 39 36.0 40 54.0 41 54.0 42 67.0 43 95.5 44 109.0 45 124.5 46 137.0 47 173.0 48 192.0 49 186.0 50 189.0 51 199.5 52 200.5 53 199.0 54 184.0 55 159.5 56 143.0 57 122.0 58 113.0 59 98.0 60 94.0 61 104.0 62 101.0 63 86.0 64 79.5 65 79.5 66 76.0 67 83.5 68 83.5 69 57.5 70 45.5 71 41.0 72 27.0 73 17.0 74 11.5 75 12.0 76 14.0 77 11.5 78 7.0 79 2.5 80 0.5 81 1.0 82 0.5 83 0.0 84 0.5 85 1.0 86 0.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 1.0 94 1.5 95 0.5 96 1.5 97 1.5 98 1.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.015 145-149 0.005 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.375 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75279106858055 66.425 2 9.728867623604467 15.25 3 3.253588516746411 7.6499999999999995 4 1.0526315789473684 3.3000000000000003 5 0.28708133971291866 1.125 6 0.22328548644338117 1.05 7 0.22328548644338117 1.225 8 0.19138755980861244 1.2 9 0.03189792663476874 0.22499999999999998 >10 0.2551834130781499 2.55 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 19 0.475 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 14 0.35000000000000003 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 13 0.325 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 13 0.325 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 11 0.27499999999999997 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 11 0.27499999999999997 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 11 0.27499999999999997 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 10 0.25 No Hit GCCAGAGGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCG 9 0.22499999999999998 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 8 0.2 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 8 0.2 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 8 0.2 No Hit GTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCA 8 0.2 No Hit AGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAA 8 0.2 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 8 0.2 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 7 0.17500000000000002 No Hit GGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGC 7 0.17500000000000002 No Hit GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGGGTCGGAA 7 0.17500000000000002 No Hit CTGGAATCGGTTCAGCCGGAGGTAGGGTCCAGTGGCCGGAAGAGCACCGC 7 0.17500000000000002 No Hit GGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTC 7 0.17500000000000002 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 7 0.17500000000000002 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 7 0.17500000000000002 No Hit GTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGA 6 0.15 No Hit CACAAACACAAACACAAACACAAACACCAAAAGCAGTAGCCAACACCAGT 6 0.15 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 6 0.15 No Hit CGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGATTC 6 0.15 No Hit ATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG 6 0.15 No Hit GTTATCTTTTCTGCTTAACGGCCTGCCAACCCTGGAATCGGTTCAGCCGG 6 0.15 No Hit GTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTAT 6 0.15 No Hit CGAAGCCAGAGGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAA 5 0.125 No Hit GGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAG 5 0.125 No Hit CTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCG 5 0.125 No Hit CTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTT 5 0.125 No Hit GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA 5 0.125 No Hit GAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGAC 5 0.125 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 5 0.125 No Hit GACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAAC 5 0.125 No Hit CAAACATCAAAGCAAACTAGTAACCAGCCACCATGAAGAGCTTGTTCTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.0875 0.0 0.0 0.0 0.0 46-47 0.125 0.0 0.0 0.0 0.0 48-49 0.2 0.0 0.0 0.0 0.0 50-51 0.21250000000000002 0.0 0.0 0.0 0.0 52-53 0.2625 0.0 0.0 0.0 0.0 54-55 0.3 0.0 0.0 0.0 0.0 56-57 0.3375 0.0 0.0 0.0 0.0 58-59 0.3625 0.0 0.0 0.0 0.0 60-61 0.4125 0.0 0.0 0.0 0.0 62-63 0.475 0.0 0.0 0.0 0.0 64-65 0.5375000000000001 0.0 0.0 0.0 0.0 66-67 0.5625 0.0 0.0 0.0 0.0 68-69 0.6125 0.0 0.0 0.0 0.0 70-71 0.775 0.0 0.0 0.0 0.0 72-73 0.9875 0.0 0.0 0.0 0.0 74-75 1.175 0.0 0.0 0.0 0.0 76-77 1.375 0.0 0.0 0.0 0.0 78-79 1.5 0.0 0.0 0.0 0.0 80-81 1.625 0.0 0.0 0.0 0.0 82-83 1.8875 0.0 0.0 0.0 0.0 84-85 2.2375 0.0 0.0 0.0 0.0 86-87 2.55 0.0 0.0 0.0 0.0 88-89 2.95 0.0 0.0 0.0 0.0 90-91 3.2874999999999996 0.0 0.0 0.0 0.0 92-93 3.825 0.0 0.0 0.0 0.0 94-95 4.4 0.0 0.0 0.0 0.0 96-97 4.9 0.0 0.0 0.0 0.0 98-99 5.35 0.0 0.0 0.0 0.0 100-101 5.8875 0.0 0.0 0.0 0.0 102-103 6.5375 0.0 0.0 0.0 0.0 104-105 7.05 0.0 0.0 0.0 0.0 106-107 7.5875 0.0 0.0 0.0 0.0 108-109 8.2 0.0 0.0 0.0 0.0 110-111 9.0125 0.0 0.0 0.0 0.0 112-113 9.75 0.0 0.0 0.0 0.0 114-115 10.5375 0.0 0.0 0.0 0.0 116-117 11.175 0.0 0.0 0.0 0.0 118-119 12.0375 0.0 0.0 0.0 0.0 120-121 12.875 0.0 0.0 0.0 0.0 122-123 13.75 0.0 0.0 0.0 0.0 124-125 14.6875 0.0 0.0 0.0 0.0 126-127 15.912500000000001 0.0 0.0 0.0 0.0 128-129 16.9375 0.0 0.0 0.0 0.0 130-131 17.9625 0.0 0.0 0.0 0.0 132-133 18.9875 0.0 0.0 0.0 0.0 134-135 19.7875 0.0 0.0 0.0 0.0 136-137 20.4625 0.0 0.0 0.0 0.0 138 21.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGCG 10 0.006973645 144.0 6 TGCGTGC 10 0.006973645 144.0 9 ATGCGTG 10 0.006973645 144.0 8 CTGACAT 10 0.006973645 144.0 3 TCTGACA 10 0.006973645 144.0 2 CATGCGT 10 0.006973645 144.0 7 GGGGGGG 485 0.0 8.313402 135-139 >>END_MODULE Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332677 spots for SRR24040058.sra Written 4332677 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra Read 4332671 spots for SRR24040058.sra Written 4332671 spots for SRR24040058.sra SRR ids: ['SRR24040058.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_oc4ui8zv SRR24040058.sra spots: 86653426 blocks: [[1, 4332671], [4332672, 8665342], [8665343, 12998013], [12998014, 17330684], [17330685, 21663355], [21663356, 25996026], [25996027, 30328697], [30328698, 34661368], [34661369, 38994039], [38994040, 43326710], [43326711, 47659381], [47659382, 51992052], [51992053, 56324723], [56324724, 60657394], [60657395, 64990065], [64990066, 69322736], [69322737, 73655407], [73655408, 77988078], [77988079, 82320749], [82320750, 86653426]] SRR24040058 file size 29257679 SRR24040058 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR24040058 SRR24040058_1.fastq SRR24040058_2.fastq Input file: SRR24040058_1.fastq Paired file: SRR24040058_2.fastq trimmed: SRR24040058-trimmed-pair1.fastq, SRR24040058-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 22:10:58 2024 >> started Fri Dec 6 22:14:21 2024 >> done (202.979s) 86653426 read pairs processed; of these: 27198 ( 0.03%) short read pairs filtered out after trimming by size control 42836 ( 0.05%) empty read pairs filtered out after trimming by size control 86583392 (99.92%) read pairs available; of these: 23142090 (26.73%) trimmed read pairs available after processing 63441302 (73.27%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1031 0.00% 19 955 0.00% 20 657 0.00% 21 773 0.00% 22 925 0.00% 23 639 0.00% 24 695 0.00% 25 832 0.00% 26 792 0.00% 27 870 0.00% 28 901 0.00% 29 994 0.00% 30 2648 0.00% 31 1127 0.00% 32 1219 0.00% 33 1375 0.00% 34 1423 0.00% 35 1599 0.00% 36 1753 0.00% 37 1851 0.00% 38 2306 0.00% 39 2807 0.00% 40 3308 0.00% 41 3775 0.00% 42 3980 0.00% 43 4351 0.01% 44 4493 0.01% 45 5042 0.01% 46 5888 0.01% 47 7095 0.01% 48 8694 0.01% 49 10220 0.01% 50 11828 0.01% 51 13068 0.02% 52 14647 0.02% 53 15303 0.02% 54 16853 0.02% 55 17711 0.02% 56 19769 0.02% 57 22110 0.03% 58 26931 0.03% 59 28863 0.03% 60 32120 0.04% 61 36272 0.04% 62 39590 0.05% 63 40789 0.05% 64 42030 0.05% 65 43445 0.05% 66 44836 0.05% 67 49952 0.06% 68 54032 0.06% 69 57289 0.07% 70 58712 0.07% 71 64730 0.07% 72 71272 0.08% 73 79055 0.09% 74 79096 0.09% 75 82720 0.10% 76 87648 0.10% 77 90115 0.10% 78 94590 0.11% 79 103284 0.12% 80 112975 0.13% 81 115944 0.13% 82 123437 0.14% 83 125207 0.14% 84 134562 0.16% 85 147422 0.17% 86 161643 0.19% 87 161853 0.19% 88 161893 0.19% 89 160246 0.19% 90 168730 0.19% 91 168613 0.19% 92 185928 0.21% 93 201533 0.23% 94 198975 0.23% 95 221070 0.26% 96 225478 0.26% 97 223860 0.26% 98 242471 0.28% 99 235156 0.27% 100 251752 0.29% 101 244626 0.28% 102 248950 0.29% 103 263580 0.30% 104 259370 0.30% 105 258157 0.30% 106 268062 0.31% 107 280845 0.32% 108 267886 0.31% 109 282815 0.33% 110 280100 0.32% 111 305139 0.35% 112 312613 0.36% 113 297986 0.34% 114 316522 0.37% 115 331967 0.38% 116 343397 0.40% 117 333485 0.39% 118 319281 0.37% 119 348957 0.40% 120 370808 0.43% 121 359299 0.41% 122 372835 0.43% 123 390059 0.45% 124 390152 0.45% 125 386832 0.45% 126 393760 0.45% 127 390948 0.45% 128 386691 0.45% 129 417348 0.48% 130 379525 0.44% 131 394416 0.46% 132 393769 0.45% 133 407686 0.47% 134 405008 0.47% 135 408323 0.47% 136 417748 0.48% 137 429243 0.50% 138 416909 0.48% 139 430074 0.50% 140 418025 0.48% 141 423546 0.49% 142 453687 0.52% 143 435222 0.50% 144 462276 0.53% 145 427956 0.49% 146 437273 0.51% 147 456644 0.53% 148 437082 0.50% 149 432782 0.50% 150 63441302 73.27% 86583392 reads passed initial QC criterion=sequence-density sequence-density=1.09 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=33 prefix-density=1.09 prefix-fanout=2.0 sequence=TTTGGCTTTGGC criterion=fanout-score sequence-density=0.38 sequence-density-rank=8 fanout-score=26.93 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=26.9 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAGGG criterion=sequence-density sequence-density=0.99 sequence-density-rank=1 fanout-score=3.09 fanout-score-rank=21 prefix-density=1.50 prefix-fanout=2.1 sequence=CCATGAAGACCTTTCTCATCCTCGCCCTCCTCGCCATCGTGGCGACCACCACCACTGCGCTGGTAGTCGACCCAGTCACTAGATCTTTTCAGCCGTCACAGGAACAATCATGCCAGCAGCC criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=236.88 fanout-score-rank=1 prefix-density=1.36 prefix-fanout=4.1 sequence=AGCAGCAGCAAGAACAGGGGCAGCAAGGTCAGATCCCTTCGCGCCGGTTCGGGGCCCAGTCACAGGAGTGCGGACAACAGGGTATCTTCTCGCCCCAGCTGCTAGCTCAGATACTACCGGCGATGTGCAATGTGTACCTCCCACCGTACTGCCGCGCCACCGCCGCTGATCAGCCATTCGGACCCGGTGGCTACTGATAAGACAAAGGGCTCTTGTAGTAGATAGATAGGGATCACCGCT SRR24040058 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 22:16:52 Started mapping on | Dec 06 22:16:52 Finished on | Dec 06 22:28:07 Mapping speed, Million of reads per hour | 461.78 Number of input reads | 86583392 Average input read length | 282 UNIQUE READS: Uniquely mapped reads number | 29343372 Uniquely mapped reads % | 33.89% Average mapped length | 282.81 Number of splices: Total | 19921782 Number of splices: Annotated (sjdb) | 18123956 Number of splices: GT/AG | 19042322 Number of splices: GC/AG | 333402 Number of splices: AT/AC | 9696 Number of splices: Non-canonical | 536362 Mismatch rate per base, % | 0.35% Deletion rate per base | 0.01% Deletion average length | 2.14 Insertion rate per base | 0.01% Insertion average length | 2.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9165533 % of reads mapped to multiple loci | 10.59% Number of reads mapped to too many loci | 7001593 % of reads mapped to too many loci | 8.09% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.13% % of reads unmapped: other | 44.30% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 48074487 48074487 48074487 N_multimapping 9165533 9165533 9165533 N_noFeature 1744727 28622624 1887859 N_ambiguous 958312 1883 599872 UnstrandedReadsAssigned:26640333 PositiveStrandReadsAssigned:718865 NegativeStrandReadsAssigned:26855641 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=135 echo kmer=131 SRR24040058 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR24040058-trimmed-pair1.fastq SRR24040058-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 86,583,392 reads, 34,307,131 reads pseudoaligned [quant] estimated average fragment length: 200.27 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,101 rounds 52973 SRR24040058.ke.tsv 35125 SRR24040058.se.tsv 88098 total ==> SRR24040058.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 736.897 93.137 3.88334 PNS24247 1044 844.73 22.1661 0.806235 PNS24249 1928 1728.73 77.51 1.37759 PNS24246 1044 844.73 22.1661 0.806235 PNS24248 1044 844.73 22.1661 0.806235 PNS24244 1471 1271.73 111.855 2.7024 PNS24243 293 119.524 0 0 KQK14069 1603 1403.73 4700.3 102.88 KQK14071 474 279.534 2.61818 0.287776 ==> SRR24040058.se.tsv <== BRADI_1g14170v3 4622 BRADI_1g53295v3 29 BRADI_1g59795v3 44 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 580 BRADI_1g74790v3 53 BRADI_1g09890v3 5 BRADI_1g77505v3 320 BRADI_1g48960v3 3 SRR24040058 completed mapping pipeline successfully