Starting /dee2/code/volunteer_pipeline.sh SRR5578451 current disk space = 1523300970496 free memory = 1575379720 SRR5578451 SRAfilesize 391932c9c48b1ed34a8ada252b022ce7 SRR5578451.sra SRR5578451.sra file validated SRR5578451 is paired end SRR5578451 is conventional basespace SRR5578451 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578451_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.2515 34.0 33.0 34.0 33.0 34.0 2 33.44075 34.0 34.0 34.0 33.0 34.0 3 33.459 34.0 34.0 34.0 33.0 34.0 4 33.47125 34.0 34.0 34.0 33.0 34.0 5 33.41975 34.0 34.0 34.0 33.0 34.0 6 37.152 38.0 38.0 38.0 36.0 38.0 7 37.33625 38.0 38.0 38.0 37.0 38.0 8 37.44575 38.0 38.0 38.0 37.0 38.0 9 37.41925 38.0 38.0 38.0 37.0 38.0 10-14 37.489000000000004 38.0 38.0 38.0 37.4 38.0 15-19 37.48485 38.0 38.0 38.0 37.8 38.0 20-24 37.5011 38.0 38.0 38.0 38.0 38.0 25-29 37.386399999999995 38.0 38.0 38.0 37.8 38.0 30-34 37.3625 38.0 38.0 38.0 37.8 38.0 35-39 37.3131 38.0 38.0 38.0 37.0 38.0 40-44 37.204699999999995 38.0 38.0 38.0 37.0 38.0 45-49 37.2232 38.0 38.0 38.0 37.0 38.0 50-54 37.148450000000004 38.0 38.0 38.0 37.0 38.0 55-59 37.10510000000001 38.0 38.0 38.0 36.8 38.0 60-64 37.05475 38.0 38.0 38.0 36.8 38.0 65-69 37.0822 38.0 38.0 38.0 37.0 38.0 70-74 36.9211 38.0 38.0 38.0 36.4 38.0 75-79 36.6731 38.0 38.0 38.0 36.0 38.0 80-84 36.608799999999995 38.0 38.0 38.0 35.8 38.0 85-89 36.579499999999996 38.0 38.0 38.0 35.4 38.0 90-94 36.4818 38.0 38.0 38.0 35.2 38.0 95-99 36.378699999999995 38.0 38.0 38.0 34.6 38.0 100-104 36.27435 38.0 38.0 38.0 34.4 38.0 105-109 36.13289999999999 38.0 38.0 38.0 34.0 38.0 110-114 36.039849999999994 38.0 38.0 38.0 34.0 38.0 115-119 35.92115 38.0 38.0 38.0 33.8 38.0 120-124 35.87284999999999 38.0 38.0 38.0 33.4 38.0 125-129 35.658 38.0 38.0 38.0 32.6 38.0 130-134 35.62625 38.0 38.0 38.0 33.0 38.0 135-139 35.4157 38.0 37.2 38.0 31.6 38.0 140-144 35.180749999999996 38.0 36.0 38.0 31.0 38.0 145-149 34.679700000000004 38.0 36.0 38.0 28.6 38.0 150-151 31.556125 36.5 32.0 38.0 13.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 2.0 6 2.0 7 3.0 8 1.0 9 5.0 10 0.0 11 2.0 12 1.0 13 3.0 14 1.0 15 4.0 16 4.0 17 2.0 18 17.0 19 19.0 20 3.0 21 5.0 22 5.0 23 8.0 24 8.0 25 10.0 26 18.0 27 10.0 28 24.0 29 17.0 30 37.0 31 29.0 32 59.0 33 77.0 34 94.0 35 168.0 36 372.0 37 2988.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 45.10445507173421 12.282909640070477 10.067958721369243 32.54467656682608 2 26.375 13.8 28.9 30.925000000000004 3 23.575 18.425 24.4 33.6 4 25.75 26.5 22.3 25.45 5 25.45 29.2 25.05 20.3 6 22.625 32.5 25.825 19.05 7 16.404101025256317 26.531632908227053 38.93473368342086 18.12953238309577 8 18.8 26.125 32.225 22.85 9 20.125 22.475 34.300000000000004 23.1 10-14 22.07 29.615000000000002 26.07 22.245 15-19 22.035 27.700000000000003 26.755000000000003 23.51 20-24 21.795 27.095000000000002 27.894999999999996 23.215 25-29 21.285 28.055000000000003 27.26 23.400000000000002 30-34 21.07 28.075 27.315 23.54 35-39 22.035 26.76 27.189999999999998 24.015 40-44 23.525 26.97 25.69 23.815 45-49 22.32 27.805000000000003 26.740000000000002 23.135 50-54 23.505000000000003 26.995 26.215 23.285 55-59 22.905 27.26 26.365 23.47 60-64 21.044999999999998 28.515 26.215 24.224999999999998 65-69 21.555 28.439999999999998 25.855 24.15 70-74 22.955000000000002 28.705000000000002 25.115 23.225 75-79 22.259999999999998 26.435 25.974999999999998 25.330000000000002 80-84 22.634999999999998 27.860000000000003 25.69 23.815 85-89 23.415 27.215 26.284999999999997 23.085 90-94 22.935 26.615 26.33 24.12 95-99 22.655 27.235 26.55 23.56 100-104 23.169999999999998 27.034999999999997 25.919999999999998 23.875 105-109 23.515 27.810000000000002 25.7 22.975 110-114 22.11 27.21 25.335 25.345000000000002 115-119 22.729545909181837 27.615523104620927 25.825165033006602 23.829765953190638 120-124 23.150000000000002 26.740000000000002 24.635 25.474999999999998 125-129 22.855 27.815 24.365000000000002 24.965 130-134 23.965 26.68 24.665 24.69 135-139 22.455 28.084999999999997 25.674999999999997 23.785 140-144 23.525 27.589999999999996 24.565 24.32 145-149 23.081154057702886 27.76138806940347 23.656182809140457 25.501275063753187 150-151 22.768192048012004 26.731682920730183 24.843710927731934 25.656414103525883 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.5 5 0.5 6 0.0 7 0.5 8 0.5 9 0.5 10 1.0 11 1.0 12 0.5 13 0.5 14 0.5 15 0.5 16 0.5 17 0.5 18 0.5 19 0.0 20 1.0 21 2.0 22 1.5 23 0.5 24 0.5 25 1.0 26 1.5 27 3.5 28 4.5 29 12.5 30 26.5 31 51.0 32 55.5 33 50.0 34 69.0 35 92.0 36 111.5 37 149.0 38 161.0 39 140.5 40 153.0 41 153.0 42 135.5 43 126.0 44 142.5 45 164.0 46 163.5 47 164.5 48 163.0 49 152.0 50 158.5 51 157.0 52 155.5 53 164.0 54 147.5 55 125.5 56 97.0 57 79.5 58 73.5 59 64.0 60 45.0 61 32.5 62 34.5 63 32.0 64 28.5 65 24.5 66 16.0 67 13.0 68 15.0 69 16.5 70 12.0 71 7.0 72 9.5 73 10.5 74 8.5 75 5.0 76 1.5 77 1.5 78 2.0 79 1.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.675 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.025 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.02 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.005 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.7 #Duplication Level Percentage of deduplicated Percentage of total 1 91.84587813620072 76.875 2 4.778972520908005 8.0 3 1.5531660692951015 3.9 4 0.6869772998805257 2.3 5 0.2688172043010753 1.125 6 0.2688172043010753 1.35 7 0.14934289127837516 0.8750000000000001 8 0.02986857825567503 0.2 9 0.02986857825567503 0.22499999999999998 >10 0.38829151732377537 5.1499999999999995 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTGATCTCGTATGC 39 0.975 TruSeq Adapter, Index 7 (97% over 36bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 27 0.675 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 23 0.575 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 17 0.42500000000000004 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 13 0.325 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 13 0.325 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 11 0.27499999999999997 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 11 0.27499999999999997 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 11 0.27499999999999997 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 11 0.27499999999999997 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 10 0.25 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 10 0.25 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 10 0.25 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 9 0.22499999999999998 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 8 0.2 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 7 0.17500000000000002 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 7 0.17500000000000002 No Hit CAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTAT 7 0.17500000000000002 No Hit CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA 7 0.17500000000000002 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 7 0.17500000000000002 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 6 0.15 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 6 0.15 No Hit GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA 6 0.15 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 6 0.15 No Hit GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA 6 0.15 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 6 0.15 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 6 0.15 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 6 0.15 No Hit GGTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAA 6 0.15 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 5 0.125 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 5 0.125 No Hit GGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAG 5 0.125 No Hit GTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCT 5 0.125 No Hit TGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATAT 5 0.125 No Hit GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC 5 0.125 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 5 0.125 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 5 0.125 No Hit GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.5375 0.0 0.0 0.0 0.0 88-89 0.6625000000000001 0.0 0.0 0.0 0.0 90-91 0.775 0.0 0.0 0.0 0.0 92-93 0.8625 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.3875000000000002 0.0 0.0 0.0 0.0 98-99 1.7125 0.0 0.0 0.0 0.0 100-101 2.1 0.0 0.0 0.0 0.0 102-103 2.4875 0.0 0.0 0.0 0.0 104-105 2.9749999999999996 0.0 0.0 0.0 0.0 106-107 3.3875 0.0 0.0 0.0 0.0 108-109 3.725 0.0 0.0 0.0 0.0 110-111 4.112500000000001 0.0 0.0 0.0 0.0 112-113 4.5 0.0 0.0 0.0 0.0 114-115 5.2125 0.0 0.0 0.0 0.0 116-117 6.1125 0.0 0.0 0.0 0.0 118-119 6.5625 0.0 0.0 0.0 0.0 120-121 7.2625 0.0 0.0 0.0 0.0 122-123 7.8875 0.0 0.0 0.0 0.0 124-125 8.600000000000001 0.0 0.0 0.0 0.0 126-127 9.35 0.0 0.0 0.0 0.0 128-129 10.35 0.0 0.0 0.0 0.0 130-131 11.024999999999999 0.0 0.0 0.0 0.0 132-133 11.9 0.0 0.0 0.0 0.0 134-135 12.9 0.0 0.0 0.0 0.0 136-137 13.8125 0.0 0.0 0.0 0.0 138-139 14.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCTTT 10 0.006832588 144.9875 145 CTTAGTT 30 1.412442E-5 96.65834 7 TAGTTAC 30 1.412442E-5 96.65834 9 ACTTAGT 30 1.412442E-5 96.65834 6 CCTCACT 30 1.412442E-5 96.65834 2 TCACTTA 30 1.412442E-5 96.65834 4 GCCTCAC 35 2.8480996E-5 83.898735 1 CACTTAG 35 3.0364467E-5 82.85 5 CTCACTT 35 3.0364467E-5 82.85 3 TTAGTTA 45 1.0553847E-4 64.43889 8 TTGTTTT 20 0.0059376103 28.9975 85-89 AAACCTC 20 0.0059376103 28.9975 105-109 GGTGATT 20 0.0059376103 28.9975 80-84 CATGATG 20 0.0059376103 28.9975 75-79 AAACCAT 20 0.0059376103 28.9975 95-99 CCTATTG 20 0.0059376103 28.9975 110-114 GAAACCT 20 0.0059376103 28.9975 105-109 GAGGAAA 20 0.0059376103 28.9975 100-104 CTCCCGT 20 0.0059376103 28.9975 125-129 GATACCT 20 0.0059376103 28.9975 120-124 >>END_MODULE SRR5578451 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578451_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.029 33.0 33.0 34.0 31.0 34.0 2 32.13925 33.0 33.0 34.0 31.0 34.0 3 32.00525 33.0 33.0 34.0 29.0 34.0 4 31.9785 33.0 33.0 34.0 30.0 34.0 5 32.047 33.0 33.0 34.0 31.0 34.0 6 36.064 38.0 38.0 38.0 33.0 38.0 7 35.99125 38.0 38.0 38.0 33.0 38.0 8 36.09875 38.0 38.0 38.0 33.0 38.0 9 36.0445 38.0 38.0 38.0 33.0 38.0 10-14 35.95555 38.0 37.8 38.0 32.6 38.0 15-19 35.80459999999999 38.0 37.6 38.0 30.8 38.0 20-24 35.63855 38.0 37.0 38.0 30.4 38.0 25-29 35.512 38.0 37.0 38.0 29.0 38.0 30-34 35.26885 38.0 37.0 38.0 28.8 38.0 35-39 35.201499999999996 38.0 37.0 38.0 28.4 38.0 40-44 35.051899999999996 38.0 36.0 38.0 28.4 38.0 45-49 34.7299 38.0 36.0 38.0 27.0 38.0 50-54 34.459199999999996 38.0 35.6 38.0 25.8 38.0 55-59 34.29665 38.0 35.2 38.0 24.8 38.0 60-64 34.01595 38.0 34.6 38.0 21.0 38.0 65-69 33.55435 38.0 34.0 38.0 16.0 38.0 70-74 33.19775 38.0 33.8 38.0 16.0 38.0 75-79 32.74309999999999 38.0 33.0 38.0 15.4 38.0 80-84 32.312050000000006 37.4 31.6 38.0 15.0 38.0 85-89 31.75965 37.0 30.0 38.0 15.0 38.0 90-94 31.0784 36.8 28.6 38.0 14.6 38.0 95-99 30.383050000000004 36.0 26.8 38.0 13.6 38.0 100-104 29.69395 35.6 24.6 38.0 13.0 38.0 105-109 28.75145 35.0 22.6 38.0 4.2 38.0 110-114 27.697900000000004 34.2 16.2 38.0 2.0 38.0 115-119 26.57465 34.0 15.0 38.0 2.0 38.0 120-124 25.59665 33.2 14.4 37.8 2.0 38.0 125-129 24.430799999999998 31.4 13.4 37.0 2.0 38.0 130-134 22.691850000000002 28.0 6.4 36.2 2.0 38.0 135-139 21.1992 24.2 2.0 35.4 2.0 38.0 140-144 19.15885 20.2 2.0 34.6 2.0 38.0 145-149 16.7558 8.8 2.0 33.6 2.0 38.0 150-151 12.521875 2.0 2.0 28.5 2.0 35.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35.0 3 5.0 4 11.0 5 8.0 6 12.0 7 7.0 8 13.0 9 9.0 10 8.0 11 14.0 12 15.0 13 14.0 14 22.0 15 18.0 16 29.0 17 42.0 18 45.0 19 42.0 20 53.0 21 63.0 22 65.0 23 81.0 24 64.0 25 102.0 26 112.0 27 113.0 28 154.0 29 186.0 30 202.0 31 254.0 32 272.0 33 347.0 34 432.0 35 475.0 36 481.0 37 195.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.35 19.625 13.200000000000001 26.825 2 31.02930127723516 23.015276734285 23.140495867768596 22.814926120711245 3 23.99198597545705 24.61808164287503 29.17605810167794 22.21387427998998 4 26.195842724768344 31.05434510393188 21.98847983971951 20.761332331580267 5 28.449787127473076 32.78236914600551 19.734535437014774 19.033308289506635 6 24.567993989481593 34.41021788129226 20.66115702479339 20.36063110443276 7 22.26396193338342 22.33909341347358 33.83420986726772 21.562734785875282 8 23.841723015276735 26.02053593789131 24.292511895817682 25.845229151014276 9 25.494615577260205 24.3175557225144 25.845229151014276 24.34259954921112 10-14 25.786415547986373 25.94670406732118 23.727709877780004 24.53917050691244 15-19 25.539802615099443 25.79530083663143 25.04884524823406 23.616051300035068 20-24 25.81307942871461 26.85041343021799 24.269606614883486 23.066900526183915 25-29 26.950070182474434 26.077802285943452 24.12271906958091 22.849408462001204 30-34 26.149295633428586 25.793352383817115 25.266957437208603 22.7903945455457 35-39 25.224232099012877 25.088941223630805 25.70025554943128 23.98657112792504 40-44 26.271228896347875 25.07389409348229 25.54481238414909 23.11006462602074 45-49 25.85913235146779 24.591724276124637 26.124636809938885 23.424506562468693 50-54 24.28979407785961 24.941129315095946 27.48133674031765 23.28773986672679 55-59 24.55155827237198 26.29020943982363 26.921535223970338 22.23669706383405 60-64 23.571858087793146 25.957105632391258 27.31509320505111 23.155943074764483 65-69 23.275862068965516 27.330593424218126 26.383319967923015 23.010224538893347 70-74 23.617234468937877 26.56813627254509 26.3376753507014 23.47695390781563 75-79 23.019094872951438 27.339247230992836 25.966020147346264 23.675637748709466 80-84 23.942461908580594 27.155172413793103 26.087610264635124 22.81475541299118 85-89 23.36340852130326 27.639097744360903 26.606516290726816 22.39097744360902 90-94 24.287217517662977 27.39389687828832 26.10111740241519 22.21776820163351 95-99 23.352873390450423 27.95731249060574 26.218748434290294 22.471065684653542 100-104 24.05190120735434 28.28014628525625 25.364460698361807 22.303491809027605 105-109 23.826950070182473 28.258472027270905 25.63665530378985 22.277922598756767 110-114 23.146337795157166 27.663307765578782 25.40231613776508 23.788038301498972 115-119 24.523952695931047 28.5277610743636 24.73441571457206 22.213870515133294 120-124 24.259287110843736 29.217426179375344 24.76562891662907 21.757657793151854 125-129 24.899759422614274 29.07979149959904 24.06776263031275 21.952686447473937 130-134 25.47358925528716 27.954294878219905 24.711837225618925 21.860278640874007 135-139 25.515063411699835 28.156799839590956 23.956088024462378 22.37204872424683 140-144 26.834402566158783 27.591218925421014 24.44867682437851 21.1257016840417 145-149 26.68302945301543 27.820076137046684 24.278701662993388 21.2181927469445 150-151 28.4748309541698 26.73428499874781 22.777360380666163 22.01352366641623 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 4.0 2 2.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 1.5 18 2.0 19 1.0 20 1.0 21 1.0 22 0.5 23 1.0 24 0.5 25 0.0 26 2.0 27 3.0 28 3.5 29 5.5 30 8.5 31 19.0 32 27.0 33 26.5 34 38.5 35 60.0 36 78.5 37 108.5 38 155.0 39 157.5 40 184.5 41 167.5 42 116.5 43 133.0 44 134.5 45 143.5 46 147.0 47 153.0 48 173.5 49 173.0 50 153.0 51 139.5 52 151.5 53 173.0 54 177.5 55 158.0 56 122.5 57 94.5 58 89.5 59 78.0 60 53.0 61 41.5 62 41.0 63 42.5 64 37.0 65 32.0 66 26.5 67 17.0 68 14.5 69 18.5 70 18.0 71 19.0 72 17.0 73 12.0 74 10.0 75 7.5 76 7.0 77 4.5 78 1.5 79 2.0 80 1.5 81 0.5 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.17500000000000002 3 0.17500000000000002 4 0.17500000000000002 5 0.17500000000000002 6 0.17500000000000002 7 0.17500000000000002 8 0.17500000000000002 9 0.17500000000000002 10-14 0.18 15-19 0.19499999999999998 20-24 0.22499999999999998 25-29 0.26 30-34 0.265 35-39 0.215 40-44 0.19499999999999998 45-49 0.19 50-54 0.20500000000000002 55-59 0.21 60-64 0.22 65-69 0.24 70-74 0.2 75-79 0.23500000000000001 80-84 0.24 85-89 0.25 90-94 0.215 95-99 0.20500000000000002 100-104 0.19499999999999998 105-109 0.26 110-114 0.265 115-119 0.22 120-124 0.265 125-129 0.24 130-134 0.22999999999999998 135-139 0.255 140-144 0.24 145-149 0.18 150-151 0.17500000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.375 #Duplication Level Percentage of deduplicated Percentage of total 1 91.9815668202765 74.85000000000001 2 4.301075268817205 7.000000000000001 3 1.5053763440860215 3.675 4 0.8294930875576038 2.7 5 0.43010752688172044 1.7500000000000002 6 0.21505376344086022 1.05 7 0.06144393241167435 0.35000000000000003 8 0.09216589861751152 0.6 9 0.06144393241167435 0.44999999999999996 >10 0.522273425499232 7.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 28 0.7000000000000001 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 26 0.65 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 25 0.625 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 25 0.625 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 22 0.5499999999999999 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 21 0.525 Illumina Single End PCR Primer 1 (100% over 50bp) GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 20 0.5 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 17 0.42500000000000004 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 16 0.4 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 16 0.4 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 15 0.375 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 15 0.375 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 15 0.375 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 11 0.27499999999999997 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 11 0.27499999999999997 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 10 0.25 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 10 0.25 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 9 0.22499999999999998 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 9 0.22499999999999998 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 8 0.2 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 8 0.2 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 8 0.2 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 7 0.17500000000000002 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 7 0.17500000000000002 No Hit CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA 6 0.15 No Hit GAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATT 6 0.15 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 6 0.15 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 6 0.15 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 6 0.15 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 6 0.15 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 6 0.15 No Hit CGACTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAG 5 0.125 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 5 0.125 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 5 0.125 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 5 0.125 No Hit AGGAGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGC 5 0.125 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 5 0.125 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 5 0.125 No Hit GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC 5 0.125 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 5 0.125 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 5 0.125 No Hit CTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTG 5 0.125 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 5 0.125 No Hit GGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATC 5 0.125 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.5125 0.0 0.0 0.0 0.0 90-91 0.6 0.0 0.0 0.0 0.0 92-93 0.6625000000000001 0.0 0.0 0.0 0.0 94-95 0.825 0.0 0.0 0.0 0.0 96-97 1.0625 0.0 0.0 0.0 0.0 98-99 1.3 0.0 0.0 0.0 0.0 100-101 1.6 0.0 0.0 0.0 0.0 102-103 1.8625 0.0 0.0 0.0 0.0 104-105 2.2125 0.0 0.0 0.0 0.0 106-107 2.5250000000000004 0.0 0.0 0.0 0.0 108-109 2.7875 0.0 0.0 0.0 0.0 110-111 3.05 0.0 0.0 0.0 0.0 112-113 3.3125 0.0 0.0 0.0 0.0 114-115 3.75 0.0 0.0 0.0 0.0 116-117 4.325 0.0 0.0 0.0 0.0 118-119 4.6625 0.0 0.0 0.0 0.0 120-121 5.074999999999999 0.0 0.0 0.0 0.0 122-123 5.4625 0.0 0.0 0.0 0.0 124-125 5.8375 0.0 0.0 0.0 0.0 126-127 6.125 0.0 0.0 0.0 0.0 128-129 6.675000000000001 0.0 0.0 0.0 0.0 130-131 6.975 0.0 0.0 0.0 0.0 132-133 7.525 0.0 0.0 0.0 0.0 134-135 8.05 0.0 0.0 0.0 0.0 136-137 8.600000000000001 0.0 0.0 0.0 0.0 138-139 8.9125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090141 spots for SRR5578451.sra Written 1090141 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra Read 1090126 spots for SRR5578451.sra Written 1090126 spots for SRR5578451.sra SRR ids: ['SRR5578451.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_id4w28vm SRR5578451.sra spots: 21802535 blocks: [[1, 1090126], [1090127, 2180252], [2180253, 3270378], [3270379, 4360504], [4360505, 5450630], [5450631, 6540756], [6540757, 7630882], [7630883, 8721008], [8721009, 9811134], [9811135, 10901260], [10901261, 11991386], [11991387, 13081512], [13081513, 14171638], [14171639, 15261764], [15261765, 16351890], [16351891, 17442016], [17442017, 18532142], [18532143, 19622268], [19622269, 20712394], [20712395, 21802535]] SRR5578451 file size 7366463 SRR5578451 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578451 SRR5578451_1.fastq SRR5578451_2.fastq Input file: SRR5578451_1.fastq Paired file: SRR5578451_2.fastq trimmed: SRR5578451-trimmed-pair1.fastq, SRR5578451-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 18:07:19 2024 >> started Mon Dec 9 18:07:45 2024 >> done (26.606s) 21802535 read pairs processed; of these: 60338 ( 0.28%) short read pairs filtered out after trimming by size control 318026 ( 1.46%) empty read pairs filtered out after trimming by size control 21424171 (98.26%) read pairs available; of these: 11951801 (55.79%) trimmed read pairs available after processing 9472370 (44.21%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 9 0.00% 20 17 0.00% 21 18 0.00% 22 26 0.00% 23 32 0.00% 24 27 0.00% 25 28 0.00% 26 30 0.00% 27 35 0.00% 28 38 0.00% 29 48 0.00% 30 42 0.00% 31 45 0.00% 32 44 0.00% 33 34 0.00% 34 39 0.00% 35 48 0.00% 36 76 0.00% 37 64 0.00% 38 68 0.00% 39 79 0.00% 40 72 0.00% 41 95 0.00% 42 92 0.00% 43 124 0.00% 44 128 0.00% 45 166 0.00% 46 209 0.00% 47 198 0.00% 48 236 0.00% 49 304 0.00% 50 304 0.00% 51 388 0.00% 52 427 0.00% 53 481 0.00% 54 487 0.00% 55 546 0.00% 56 653 0.00% 57 752 0.00% 58 870 0.00% 59 885 0.00% 60 1138 0.01% 61 1251 0.01% 62 1533 0.01% 63 1626 0.01% 64 1813 0.01% 65 2135 0.01% 66 2532 0.01% 67 3660 0.02% 68 4756 0.02% 69 8680 0.04% 70 9332 0.04% 71 5682 0.03% 72 5319 0.02% 73 5649 0.03% 74 6221 0.03% 75 6969 0.03% 76 7297 0.03% 77 8273 0.04% 78 9066 0.04% 79 10161 0.05% 80 10967 0.05% 81 12707 0.06% 82 14799 0.07% 83 16177 0.08% 84 20387 0.10% 85 23156 0.11% 86 24559 0.11% 87 27029 0.13% 88 28468 0.13% 89 30127 0.14% 90 32013 0.15% 91 32382 0.15% 92 34984 0.16% 93 37105 0.17% 94 38573 0.18% 95 40802 0.19% 96 42750 0.20% 97 44481 0.21% 98 46255 0.22% 99 48890 0.23% 100 51871 0.24% 101 54149 0.25% 102 56450 0.26% 103 60100 0.28% 104 63140 0.29% 105 66402 0.31% 106 69267 0.32% 107 71217 0.33% 108 73457 0.34% 109 73655 0.34% 110 75203 0.35% 111 78762 0.37% 112 83439 0.39% 113 90630 0.42% 114 94813 0.44% 115 98877 0.46% 116 99425 0.46% 117 99316 0.46% 118 100604 0.47% 119 101206 0.47% 120 106201 0.50% 121 107416 0.50% 122 112830 0.53% 123 117291 0.55% 124 122237 0.57% 125 124250 0.58% 126 128405 0.60% 127 128398 0.60% 128 127441 0.59% 129 133620 0.62% 130 134503 0.63% 131 136565 0.64% 132 141151 0.66% 133 145543 0.68% 134 149857 0.70% 135 152614 0.71% 136 155731 0.73% 137 160007 0.75% 138 167823 0.78% 139 173788 0.81% 140 181237 0.85% 141 187393 0.87% 142 207692 0.97% 143 218509 1.02% 144 237991 1.11% 145 270518 1.26% 146 309787 1.45% 147 385039 1.80% 148 519024 2.42% 149 887529 4.14% 150 3541454 16.53% 151 9472370 44.21% 21424171 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=22.02 fanout-score-rank=3 prefix-density=5.31 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=59.20 fanout-score-rank=1 prefix-density=0.44 prefix-fanout=1.4 sequence=TTTTTTTTCTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATTCCCGAGGCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCCCAGAGACGGGCGGAGGCTCTTAACCGTACGAGACAATCTCCA criterion=sequence-density sequence-density=0.79 sequence-density-rank=1 fanout-score=4.74 fanout-score-rank=17 prefix-density=3.71 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.08 sequence-density-rank=24 fanout-score=119.11 fanout-score-rank=1 prefix-density=9.38 prefix-fanout=1.0 sequence=CCCAACTATACAAAAGAATATCC SRR5578451 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 18:09:24 Started mapping on | Dec 09 18:09:24 Finished on | Dec 09 18:45:24 Mapping speed, Million of reads per hour | 35.71 Number of input reads | 21424171 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 10795645 Uniquely mapped reads % | 50.39% Average mapped length | 282.00 Number of splices: Total | 6802249 Number of splices: Annotated (sjdb) | 6345638 Number of splices: GT/AG | 6705931 Number of splices: GC/AG | 86216 Number of splices: AT/AC | 3011 Number of splices: Non-canonical | 7091 Mismatch rate per base, % | 0.14% Deletion rate per base | 0.01% Deletion average length | 1.48 Insertion rate per base | 0.00% Insertion average length | 1.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 218609 % of reads mapped to multiple loci | 1.02% Number of reads mapped to too many loci | 37710 % of reads mapped to too many loci | 0.18% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 47.57% % of reads unmapped: other | 0.84% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10429413 10429413 10429413 N_multimapping 218609 218609 218609 N_noFeature 337569 10363495 465441 N_ambiguous 333565 1222 30415 UnstrandedReadsAssigned:10124511 PositiveStrandReadsAssigned:430928 NegativeStrandReadsAssigned:10299789 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=141 echo kmer=137 SRR5578451 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578451-trimmed-pair1.fastq SRR5578451-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,424,171 reads, 10,428,361 reads pseudoaligned [quant] estimated average fragment length: 199.859 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,217 rounds 52973 SRR5578451.ke.tsv 35125 SRR5578451.se.tsv 88098 total ==> SRR5578451.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 737.285 0 0 PNS24247 1044 845.141 1.26819 0.189213 PNS24249 1928 1729.14 29.5278 2.15326 PNS24246 1044 845.141 1.26819 0.189213 PNS24248 1044 845.141 1.26819 0.189213 PNS24244 1471 1272.14 221.668 21.9717 PNS24243 293 118.175 0 0 KQK14069 1603 1404.14 2052.55 184.323 KQK14071 474 280.185 19.236 8.65696 ==> SRR5578451.se.tsv <== BRADI_1g14170v3 2087 BRADI_1g53295v3 74 BRADI_1g59795v3 79 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 260 BRADI_1g74790v3 300 BRADI_1g09890v3 0 BRADI_1g77505v3 111 BRADI_1g48960v3 0 SRR5578451 completed mapping pipeline successfully