Starting /dee2/code/volunteer_pipeline.sh SRR5578453 current disk space = 1522956070912 free memory = 1568759600 SRR5578453 SRAfilesize 1223920a541a76f0c4ace40d5b19a476 SRR5578453.sra SRR5578453.sra file validated SRR5578453 is paired end SRR5578453 is conventional basespace SRR5578453 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578453_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64825 34.0 33.0 34.0 33.0 34.0 2 33.39575 34.0 34.0 34.0 33.0 34.0 3 33.42175 34.0 34.0 34.0 33.0 34.0 4 33.476 34.0 34.0 34.0 33.0 34.0 5 33.53075 34.0 34.0 34.0 33.0 34.0 6 37.14075 38.0 38.0 38.0 36.0 38.0 7 37.4185 38.0 38.0 38.0 37.0 38.0 8 37.47275 38.0 38.0 38.0 37.0 38.0 9 37.522 38.0 38.0 38.0 38.0 38.0 10-14 37.52815 38.0 38.0 38.0 38.0 38.0 15-19 37.532799999999995 38.0 38.0 38.0 38.0 38.0 20-24 37.50385 38.0 38.0 38.0 38.0 38.0 25-29 37.40385 38.0 38.0 38.0 38.0 38.0 30-34 37.3817 38.0 38.0 38.0 38.0 38.0 35-39 37.23995 38.0 38.0 38.0 37.2 38.0 40-44 36.9058 38.0 38.0 38.0 36.0 38.0 45-49 37.14829999999999 38.0 38.0 38.0 36.8 38.0 50-54 37.112 38.0 38.0 38.0 36.8 38.0 55-59 37.0026 38.0 38.0 38.0 36.0 38.0 60-64 36.89065000000001 38.0 38.0 38.0 36.0 38.0 65-69 36.859249999999996 38.0 38.0 38.0 35.8 38.0 70-74 36.36005 38.0 38.0 38.0 34.4 38.0 75-79 35.26165 38.0 38.0 38.0 32.2 38.0 80-84 34.97595 38.0 38.0 38.0 29.4 38.0 85-89 34.77395 38.0 37.2 38.0 28.6 38.0 90-94 34.55355 38.0 37.0 38.0 27.4 38.0 95-99 34.4369 38.0 36.8 38.0 27.0 38.0 100-104 34.15105 38.0 36.0 38.0 24.4 38.0 105-109 33.93165 38.0 36.0 38.0 21.0 38.0 110-114 33.52535 38.0 35.0 38.0 16.2 38.0 115-119 33.28685 38.0 34.6 38.0 15.0 38.0 120-124 32.831450000000004 38.0 34.0 38.0 14.8 38.0 125-129 32.1865 38.0 33.2 38.0 14.0 38.0 130-134 31.8226 37.8 32.6 38.0 13.4 38.0 135-139 31.236 36.6 31.0 38.0 13.0 38.0 140-144 30.56105 36.0 29.4 38.0 6.4 38.0 145-149 29.038300000000003 35.0 24.8 38.0 2.0 38.0 150-151 24.067125 31.5 11.5 35.5 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 2.0 7 3.0 8 2.0 9 3.0 10 1.0 11 0.0 12 7.0 13 2.0 14 1.0 15 12.0 16 10.0 17 23.0 18 73.0 19 84.0 20 10.0 21 16.0 22 11.0 23 7.0 24 21.0 25 18.0 26 27.0 27 19.0 28 29.0 29 30.0 30 37.0 31 66.0 32 88.0 33 102.0 34 191.0 35 399.0 36 1069.0 37 1635.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 49.24222964294888 11.19958900590804 10.043668122270741 29.514513228872335 2 26.950000000000003 16.1 28.925 28.025 3 23.46760070052539 17.563172379284463 28.246184638478862 30.723042281711283 4 24.0 24.125 23.9 27.975 5 28.4 27.35 24.825 19.425 6 24.668501376032022 31.548661496122094 25.369026770077557 18.413810357768327 7 15.225 26.200000000000003 41.4 17.175 8 19.55 27.250000000000004 32.074999999999996 21.125 9 23.025000000000002 22.075 34.300000000000004 20.599999999999998 10-14 22.845 27.884999999999998 26.645000000000003 22.625 15-19 22.27 25.885 27.744999999999997 24.099999999999998 20-24 22.95 25.85 28.24 22.96 25-29 21.279999999999998 26.840000000000003 29.07 22.81 30-34 21.224999999999998 26.784999999999997 27.04 24.95 35-39 22.81 26.055 27.584999999999997 23.549999999999997 40-44 23.105 25.44 28.084999999999997 23.369999999999997 45-49 24.474999999999998 26.245 28.33 20.95 50-54 24.29 25.380000000000003 26.169999999999998 24.16 55-59 22.555 25.650000000000002 28.53 23.265 60-64 21.165 27.775 27.455000000000002 23.605 65-69 20.435 31.11 25.919999999999998 22.535 70-74 21.865000000000002 30.42 24.435000000000002 23.28 75-79 21.865000000000002 29.005 25.314999999999998 23.815 80-84 22.845 27.96 26.009999999999998 23.185 85-89 23.080000000000002 26.765 27.175 22.98 90-94 23.285 26.484999999999996 27.250000000000004 22.98 95-99 21.23 26.810000000000002 28.144999999999996 23.815 100-104 22.93 28.15 26.07 22.85 105-109 22.650000000000002 30.0 25.195 22.155 110-114 21.375 28.48 25.415 24.73 115-119 22.225 27.62 25.955000000000002 24.2 120-124 23.215 28.615000000000002 24.555 23.615 125-129 21.98 29.335 24.295 24.39 130-134 22.505 29.45 23.494999999999997 24.55 135-139 21.57 29.770000000000003 25.575 23.085 140-144 22.6 29.854999999999997 24.51 23.035 145-149 22.58 30.264999999999997 23.43 23.724999999999998 150-151 21.2625 28.6875 24.325 25.724999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 2.0 2 1.5 3 1.0 4 1.0 5 1.5 6 3.0 7 2.0 8 2.0 9 2.0 10 1.0 11 1.5 12 1.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 2.0 20 2.0 21 1.5 22 1.5 23 1.0 24 1.5 25 1.0 26 2.0 27 3.5 28 8.5 29 24.5 30 40.5 31 64.0 32 59.5 33 54.5 34 73.5 35 85.5 36 128.0 37 173.0 38 179.5 39 150.5 40 146.5 41 144.0 42 125.5 43 125.0 44 131.5 45 133.5 46 140.5 47 145.0 48 138.5 49 155.5 50 152.0 51 138.5 52 134.5 53 141.0 54 150.0 55 128.5 56 97.5 57 79.5 58 73.0 59 69.0 60 57.5 61 40.0 62 34.0 63 34.5 64 31.5 65 24.0 66 20.0 67 21.0 68 19.5 69 18.0 70 15.0 71 12.0 72 11.0 73 9.5 74 7.5 75 5.5 76 3.5 77 1.5 78 1.5 79 1.5 80 1.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.675 2 0.0 3 0.075 4 0.0 5 0.0 6 0.075 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.875 #Duplication Level Percentage of deduplicated Percentage of total 1 89.04841402337229 66.675 2 5.776293823038397 8.649999999999999 3 2.0701168614357264 4.65 4 1.2687813021702838 3.8 5 0.5342237061769616 2.0 6 0.333889816360601 1.5 7 0.23372287145242068 1.225 8 0.1335559265442404 0.8 9 0.10016694490818029 0.675 >10 0.46744574290484137 6.125 >50 0.0 0.0 >100 0.0333889816360601 3.9 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC 156 3.9 TruSeq Adapter, Index 22 (98% over 50bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 38 0.95 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 36 0.8999999999999999 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 27 0.675 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 18 0.44999999999999996 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 16 0.4 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 15 0.375 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 14 0.35000000000000003 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 13 0.325 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 12 0.3 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 12 0.3 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 12 0.3 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 11 0.27499999999999997 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 11 0.27499999999999997 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 10 0.25 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 9 0.22499999999999998 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 9 0.22499999999999998 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 9 0.22499999999999998 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 8 0.2 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 8 0.2 No Hit GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA 8 0.2 No Hit GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA 8 0.2 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 7 0.17500000000000002 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 7 0.17500000000000002 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 7 0.17500000000000002 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 7 0.17500000000000002 No Hit TGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATAT 7 0.17500000000000002 No Hit CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT 7 0.17500000000000002 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG 7 0.17500000000000002 TruSeq Adapter, Index 22 (97% over 49bp) CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA 6 0.15 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 6 0.15 No Hit GATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAG 6 0.15 No Hit GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT 6 0.15 No Hit CAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTAT 6 0.15 No Hit TCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTG 6 0.15 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 6 0.15 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 6 0.15 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6 0.15 No Hit GATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATG 6 0.15 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 5 0.125 No Hit GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA 5 0.125 No Hit CCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAA 5 0.125 No Hit GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA 5 0.125 No Hit GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCC 5 0.125 No Hit GGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACC 5 0.125 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 5 0.125 No Hit GTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAAT 5 0.125 No Hit TGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT 5 0.125 No Hit CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA 5 0.125 No Hit CATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAA 5 0.125 No Hit CACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG 5 0.125 No Hit CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA 5 0.125 No Hit GGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAG 5 0.125 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 5 0.125 No Hit GTTTACCGTGGGCTCAATGCCGGCAATCCACAGATAATTTTAGTATCTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.175 0.0 0.0 0.0 0.0 2 0.175 0.0 0.0 0.0 0.0 3 0.175 0.0 0.0 0.0 0.0 4 0.175 0.0 0.0 0.0 0.0 5 0.175 0.0 0.0 0.0 0.0 6 0.175 0.0 0.0 0.0 0.0 7 0.175 0.0 0.0 0.0 0.0 8 0.175 0.0 0.0 0.0 0.0 9 0.175 0.0 0.0 0.0 0.0 10-11 0.175 0.0 0.0 0.0 0.0 12-13 0.175 0.0 0.0 0.0 0.0 14-15 0.175 0.0 0.0 0.0 0.0 16-17 0.175 0.0 0.0 0.0 0.0 18-19 0.175 0.0 0.0 0.0 0.0 20-21 0.175 0.0 0.0 0.0 0.0 22-23 0.175 0.0 0.0 0.0 0.0 24-25 0.175 0.0 0.0 0.0 0.0 26-27 0.1875 0.0 0.0 0.0 0.0 28-29 0.2 0.0 0.0 0.0 0.0 30-31 0.2 0.0 0.0 0.0 0.0 32-33 0.2 0.0 0.0 0.0 0.0 34-35 0.2 0.0 0.0 0.0 0.0 36-37 0.2 0.0 0.0 0.0 0.0 38-39 0.2 0.0 0.0 0.0 0.0 40-41 0.2 0.0 0.0 0.0 0.0 42-43 0.2 0.0 0.0 0.0 0.0 44-45 0.2 0.0 0.0 0.0 0.0 46-47 0.2 0.0 0.0 0.0 0.0 48-49 0.2 0.0 0.0 0.0 0.0 50-51 0.2 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.2 0.0 0.0 0.0 0.0 56-57 0.2 0.0 0.0 0.0 0.0 58-59 0.2 0.0 0.0 0.0 0.0 60-61 0.2 0.0 0.0 0.0 0.0 62-63 0.2 0.0 0.0 0.0 0.0 64-65 0.2 0.0 0.0 0.0 0.0 66-67 0.225 0.0 0.0 0.0 0.0 68-69 0.25 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.35 0.0 0.0 0.0 0.0 74-75 0.42500000000000004 0.0 0.0 0.0 0.0 76-77 0.475 0.0 0.0 0.0 0.0 78-79 0.5125 0.0 0.0 0.0 0.0 80-81 0.6125 0.0 0.0 0.0 0.0 82-83 0.675 0.0 0.0 0.0 0.0 84-85 0.8125 0.0 0.0 0.0 0.0 86-87 1.0 0.0 0.0 0.0 0.0 88-89 1.1749999999999998 0.0 0.0 0.0 0.0 90-91 1.275 0.0 0.0 0.0 0.0 92-93 1.5125 0.0 0.0 0.0 0.0 94-95 1.7125 0.0 0.0 0.0 0.0 96-97 1.975 0.0 0.0 0.0 0.0 98-99 2.2375 0.0 0.0 0.0 0.0 100-101 2.7625 0.0 0.0 0.0 0.0 102-103 3.3 0.0 0.0 0.0 0.0 104-105 3.8125 0.0 0.0 0.0 0.0 106-107 4.6 0.0 0.0 0.0 0.0 108-109 5.199999999999999 0.0 0.0 0.0 0.0 110-111 5.8125 0.0 0.0 0.0 0.0 112-113 6.375 0.0 0.0 0.0 0.0 114-115 6.875 0.0 0.0 0.0 0.0 116-117 7.4625 0.0 0.0 0.0 0.0 118-119 8.1125 0.0 0.0 0.0 0.0 120-121 8.75 0.0 0.0 0.0 0.0 122-123 9.475000000000001 0.0 0.0 0.0 0.0 124-125 10.45 0.0 0.0 0.0 0.0 126-127 11.45 0.0 0.0 0.0 0.0 128-129 12.45 0.0 0.0 0.0 0.0 130-131 13.3 0.0 0.0 0.0 0.0 132-133 13.9875 0.0 0.0 0.0 0.0 134-135 14.9625 0.0 0.0 0.0 0.0 136-137 15.8125 0.0 0.0 0.0 0.0 138-139 16.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAAAC 10 0.0063298983 148.6923 1 TTGTCAC 10 0.0068343505 144.975 9 CCTTGTC 10 0.0068343505 144.975 7 ACCTTGT 10 0.0068343505 144.975 6 CTTGTCA 10 0.0068343505 144.975 8 GAGCACA 80 5.7043508E-9 63.426563 9 AAGAGCA 90 1.4473699E-8 56.379166 7 GAAGAGC 90 1.4473699E-8 56.379166 6 TCGGAAG 90 1.4473699E-8 56.379166 3 CGGAAGA 90 1.4473699E-8 56.379166 4 AGAGCAC 90 1.4473699E-8 56.379166 8 ATCGGAA 90 1.4473699E-8 56.379166 2 GGAAGAG 90 1.4473699E-8 56.379166 5 GATCGGA 100 2.6982889E-8 52.04231 1 GTATGCC 35 3.5421672E-6 28.995 45-49 TGCCGTC 35 3.5421672E-6 28.995 45-49 TATGCCG 35 3.5421672E-6 28.995 45-49 CCGTCTT 35 3.5421672E-6 28.995 50-54 TCTGCTT 35 3.5421672E-6 28.995 55-59 ATGCCGT 35 3.5421672E-6 28.995 45-49 >>END_MODULE SRR5578453 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578453_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.834 33.0 33.0 34.0 32.0 34.0 2 32.89 34.0 33.0 34.0 32.0 34.0 3 32.86075 34.0 33.0 34.0 32.0 34.0 4 32.76575 34.0 33.0 34.0 32.0 34.0 5 32.82525 34.0 33.0 34.0 32.0 34.0 6 36.967 38.0 38.0 38.0 37.0 38.0 7 36.9395 38.0 38.0 38.0 37.0 38.0 8 36.9155 38.0 38.0 38.0 37.0 38.0 9 36.94675 38.0 38.0 38.0 37.0 38.0 10-14 36.94575 38.0 38.0 38.0 37.0 38.0 15-19 36.873900000000006 38.0 38.0 38.0 37.0 38.0 20-24 36.82335 38.0 38.0 38.0 36.8 38.0 25-29 36.83795 38.0 38.0 38.0 37.0 38.0 30-34 36.8192 38.0 38.0 38.0 36.8 38.0 35-39 36.7484 38.0 38.0 38.0 36.6 38.0 40-44 36.757349999999995 38.0 38.0 38.0 36.6 38.0 45-49 36.6483 38.0 38.0 38.0 35.8 38.0 50-54 36.5867 38.0 38.0 38.0 35.8 38.0 55-59 36.6269 38.0 38.0 38.0 35.8 38.0 60-64 36.72234999999999 38.0 38.0 38.0 36.0 38.0 65-69 36.119699999999995 38.0 38.0 38.0 34.8 38.0 70-74 35.07185 38.0 38.0 38.0 31.2 38.0 75-79 35.02905 38.0 38.0 38.0 30.8 38.0 80-84 34.94835 38.0 38.0 38.0 30.2 38.0 85-89 34.867200000000004 38.0 38.0 38.0 29.6 38.0 90-94 34.81905 38.0 38.0 38.0 29.6 38.0 95-99 34.60515 38.0 38.0 38.0 27.8 38.0 100-104 34.460249999999995 38.0 37.8 38.0 26.8 38.0 105-109 34.317400000000006 38.0 37.4 38.0 24.4 38.0 110-114 34.138099999999994 38.0 37.0 38.0 22.6 38.0 115-119 33.89415 38.0 36.2 38.0 16.2 38.0 120-124 33.685900000000004 38.0 35.8 38.0 16.2 38.0 125-129 33.2847 38.0 35.0 38.0 14.4 38.0 130-134 32.956599999999995 38.0 35.0 38.0 14.0 38.0 135-139 32.3923 38.0 33.2 38.0 13.2 38.0 140-144 31.71295 38.0 33.0 38.0 6.4 38.0 145-149 30.361400000000003 38.0 30.2 38.0 2.0 38.0 150-151 25.629125000000002 34.5 16.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 3.0 4 7.0 5 4.0 6 4.0 7 0.0 8 3.0 9 3.0 10 3.0 11 4.0 12 5.0 13 8.0 14 6.0 15 14.0 16 25.0 17 130.0 18 18.0 19 3.0 20 6.0 21 11.0 22 10.0 23 16.0 24 13.0 25 18.0 26 14.0 27 18.0 28 24.0 29 34.0 30 46.0 31 49.0 32 58.0 33 89.0 34 118.0 35 250.0 36 557.0 37 2406.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.6 18.85 13.8 24.75 2 27.845884413309985 27.970978233675257 23.167375531648737 21.015761821366024 3 23.942957217913435 23.042281711283465 31.5986990242682 21.4160620465349 4 23.979974968710888 30.062578222778473 22.65331664580726 23.30413016270338 5 30.522892169126848 31.22341756317238 20.140105078809107 18.11358518889167 6 27.045283962972228 31.94896172129097 20.915686765073804 20.090067550662997 7 19.93995496622467 25.21891418563923 33.675256442331744 21.165874405804352 8 21.725 28.65 24.3 25.324999999999996 9 26.26970227670753 24.193144858643983 26.019514635976982 23.517638228671505 10-14 25.901081297557067 26.411694032839407 22.777332799359232 24.909891870244294 15-19 25.679061842237143 25.8494537436103 25.08269018743109 23.38879422672146 20-24 26.191311319336574 27.589317031617977 23.64583855288871 22.573533096156737 25-29 26.786967418546364 28.040100250626566 22.93734335839599 22.235588972431078 30-34 26.81777153745863 26.251128271988765 25.03259452411995 21.898505666432655 35-39 24.99749323172566 24.440990674822018 26.306026270931515 24.255489822520808 40-44 28.296785821568037 24.752177831180536 25.357965354961447 21.593070992289977 45-49 24.789747697236685 24.75470564677613 25.861033239887867 24.59451341609932 50-54 23.83074611917877 24.45668502754131 28.12719078617927 23.585378067100653 55-59 22.329610896890177 26.851620011017076 28.569282387700934 22.249486704391806 60-64 21.834586466165415 28.812030075187973 26.66165413533835 22.69172932330827 65-69 22.358131140966513 29.71225185482254 26.077802285943452 21.851814718267494 70-74 22.143680753998098 29.77891412242443 25.41735599338246 22.660049130195016 75-79 22.474164743654057 29.54249021771847 25.132938697702418 22.85040634092505 80-84 22.405056940751518 29.227913510259366 25.20443485677018 23.16259469221893 85-89 22.770293955631228 28.824678251289498 26.385898142120283 22.019129650958984 90-94 22.976422886319266 29.068428692996946 25.684537217800468 22.270611202883316 95-99 22.917710734689788 29.207176505963716 25.824396111055425 22.05071664829107 100-104 23.404681938944307 29.525289488194893 24.823299413504436 22.24672915935636 105-109 23.319464634818786 30.10677226928668 24.647852022657776 21.925911073236755 110-114 22.577247191011235 30.84871589085072 23.776083467094704 22.797953451043337 115-119 23.576012835940634 30.956678700361014 23.676293622141998 21.791014841556358 120-124 23.831728840754113 30.40513437625351 24.343160850381068 21.41997593261131 125-129 24.420936528627294 30.667803068284368 23.56362177880277 21.34763862428557 130-134 25.468389940887686 29.325718865845108 24.256086564472497 20.94980462879471 135-139 24.114114114114113 30.025025025025027 24.40940940940941 21.45145145145145 140-144 26.20465349011759 28.901676257192893 24.678508881661244 20.21516137102827 145-149 27.05611453171147 28.68799118986835 24.317965660509586 19.937928617910597 150-151 27.960485181943227 27.710391396773794 23.608853319995 20.720270101287984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 1.5 3 0.5 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.5 16 0.5 17 0.0 18 0.5 19 1.5 20 2.5 21 2.0 22 1.5 23 2.5 24 2.5 25 1.0 26 2.0 27 5.5 28 7.5 29 7.0 30 17.5 31 33.0 32 40.5 33 43.0 34 49.0 35 64.5 36 97.0 37 144.0 38 161.5 39 179.5 40 252.5 41 200.5 42 106.5 43 113.0 44 115.5 45 115.5 46 125.5 47 137.5 48 129.0 49 132.0 50 129.0 51 134.5 52 157.0 53 152.5 54 164.0 55 163.5 56 129.5 57 101.0 58 88.0 59 83.0 60 59.0 61 42.5 62 37.5 63 33.5 64 33.5 65 28.5 66 19.5 67 18.5 68 20.5 69 20.5 70 16.0 71 10.5 72 11.5 73 11.5 74 9.0 75 8.0 76 8.0 77 4.0 78 2.0 79 1.0 80 0.5 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.075 4 0.125 5 0.075 6 0.075 7 0.075 8 0.0 9 0.075 10-14 0.12 15-19 0.22999999999999998 20-24 0.215 25-29 0.25 30-34 0.29 35-39 0.27 40-44 0.13 45-49 0.12 50-54 0.15 55-59 0.155 60-64 0.25 65-69 0.26 70-74 0.265 75-79 0.33 80-84 0.335 85-89 0.155 90-94 0.11499999999999999 95-99 0.22999999999999998 100-104 0.255 105-109 0.255 110-114 0.32 115-119 0.27999999999999997 120-124 0.27999999999999997 125-129 0.27 130-134 0.19 135-139 0.1 140-144 0.075 145-149 0.11499999999999999 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.89999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 88.18758815232722 62.525 2 6.734837799717913 9.55 3 1.904090267983075 4.05 4 0.9167842031029618 2.6 5 0.4231311706629055 1.5 6 0.5289139633286318 2.25 7 0.21156558533145275 1.05 8 0.21156558533145275 1.2 9 0.07052186177715092 0.44999999999999996 >10 0.7757404795486601 10.7 >50 0.0 0.0 >100 0.03526093088857546 4.125 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 165 4.125 Illumina Single End PCR Primer 1 (100% over 50bp) CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 45 1.125 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 37 0.9249999999999999 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 26 0.65 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 26 0.65 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 24 0.6 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 22 0.5499999999999999 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 22 0.5499999999999999 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 22 0.5499999999999999 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 22 0.5499999999999999 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 22 0.5499999999999999 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 21 0.525 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 20 0.5 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 15 0.375 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 14 0.35000000000000003 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 13 0.325 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 12 0.3 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 12 0.3 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 12 0.3 No Hit CACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTAC 11 0.27499999999999997 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 10 0.25 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 10 0.25 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 10 0.25 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 9 0.22499999999999998 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 9 0.22499999999999998 No Hit TGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACA 8 0.2 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 8 0.2 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 8 0.2 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 8 0.2 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 8 0.2 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 8 0.2 No Hit CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA 7 0.17500000000000002 No Hit CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG 7 0.17500000000000002 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 7 0.17500000000000002 No Hit GTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTC 7 0.17500000000000002 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 7 0.17500000000000002 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 7 0.17500000000000002 Illumina Single End PCR Primer 1 (100% over 50bp) GGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC 6 0.15 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 6 0.15 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 6 0.15 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 6 0.15 No Hit GGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTTACT 6 0.15 No Hit GGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTT 6 0.15 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 6 0.15 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 6 0.15 No Hit GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG 6 0.15 No Hit TAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA 6 0.15 No Hit GGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTC 6 0.15 No Hit GTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCC 6 0.15 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 6 0.15 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 6 0.15 No Hit CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG 6 0.15 No Hit CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT 5 0.125 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 5 0.125 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 5 0.125 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 5 0.125 No Hit GACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCA 5 0.125 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 5 0.125 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 5 0.125 No Hit GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT 5 0.125 No Hit GTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTG 5 0.125 No Hit GGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAA 5 0.125 No Hit CTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATC 5 0.125 No Hit TCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.175 0.0 0.0 0.0 0.0 2 0.175 0.0 0.0 0.0 0.0 3 0.175 0.0 0.0 0.0 0.0 4 0.175 0.0 0.0 0.0 0.0 5 0.175 0.0 0.0 0.0 0.0 6 0.175 0.0 0.0 0.0 0.0 7 0.175 0.0 0.0 0.0 0.0 8 0.175 0.0 0.0 0.0 0.0 9 0.175 0.0 0.0 0.0 0.0 10-11 0.175 0.0 0.0 0.0 0.0 12-13 0.175 0.0 0.0 0.0 0.0 14-15 0.175 0.0 0.0 0.0 0.0 16-17 0.175 0.0 0.0 0.0 0.0 18-19 0.175 0.0 0.0 0.0 0.0 20-21 0.175 0.0 0.0 0.0 0.0 22-23 0.175 0.0 0.0 0.0 0.0 24-25 0.175 0.0 0.0 0.0 0.0 26-27 0.1875 0.0 0.0 0.0 0.0 28-29 0.2 0.0 0.0 0.0 0.0 30-31 0.2 0.0 0.0 0.0 0.0 32-33 0.2 0.0 0.0 0.0 0.0 34-35 0.2 0.0 0.0 0.0 0.0 36-37 0.2 0.0 0.0 0.0 0.0 38-39 0.2 0.0 0.0 0.0 0.0 40-41 0.2 0.0 0.0 0.0 0.0 42-43 0.2 0.0 0.0 0.0 0.0 44-45 0.2 0.0 0.0 0.0 0.0 46-47 0.2 0.0 0.0 0.0 0.0 48-49 0.2 0.0 0.0 0.0 0.0 50-51 0.2 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.2 0.0 0.0 0.0 0.0 56-57 0.2 0.0 0.0 0.0 0.0 58-59 0.2 0.0 0.0 0.0 0.0 60-61 0.2 0.0 0.0 0.0 0.0 62-63 0.2 0.0 0.0 0.0 0.0 64-65 0.2 0.0 0.0 0.0 0.0 66-67 0.225 0.0 0.0 0.0 0.0 68-69 0.25 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.35 0.0 0.0 0.0 0.0 74-75 0.4 0.0 0.0 0.0 0.0 76-77 0.45 0.0 0.0 0.0 0.0 78-79 0.4875 0.0 0.0 0.0 0.0 80-81 0.5875 0.0 0.0 0.0 0.0 82-83 0.65 0.0 0.0 0.0 0.0 84-85 0.7875 0.0 0.0 0.0 0.0 86-87 1.0125 0.0 0.0 0.0 0.0 88-89 1.1625 0.0 0.0 0.0 0.0 90-91 1.25 0.0 0.0 0.0 0.0 92-93 1.4625 0.0 0.0 0.0 0.0 94-95 1.6625 0.0 0.0 0.0 0.0 96-97 1.9249999999999998 0.0 0.0 0.0 0.0 98-99 2.2375 0.0 0.0 0.0 0.0 100-101 2.7750000000000004 0.0 0.0 0.0 0.0 102-103 3.3875 0.0 0.0 0.0 0.0 104-105 3.95 0.0 0.0 0.0 0.0 106-107 4.7375 0.0 0.0 0.0 0.0 108-109 5.3875 0.0 0.0 0.0 0.0 110-111 6.0 0.0 0.0 0.0 0.0 112-113 6.6 0.0 0.0 0.0 0.0 114-115 7.1 0.0 0.0 0.0 0.0 116-117 7.75 0.0 0.0 0.0 0.0 118-119 8.425 0.0 0.0 0.0 0.0 120-121 9.0375 0.0 0.0 0.0 0.0 122-123 9.7625 0.0 0.0 0.0 0.0 124-125 10.6875 0.0 0.0 0.0 0.0 126-127 11.625 0.0 0.0 0.0 0.0 128-129 12.5875 0.0 0.0 0.0 0.0 130-131 13.412500000000001 0.0 0.0 0.0 0.0 132-133 14.1875 0.0 0.0 0.0 0.0 134-135 15.175 0.0 0.0 0.0 0.0 136-137 16.1125 0.0 0.0 0.0 0.0 138-139 17.275 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAATTA 25 5.9632075E-6 114.96999 2 AGGGCTG 30 1.4757959E-5 95.808334 8 ATTAATT 30 1.4757959E-5 95.808334 1 TAATTAG 30 1.4757959E-5 95.808334 3 TAGGGCT 30 1.4757959E-5 95.808334 7 ATTAGGG 35 3.1724914E-5 82.12143 5 AATTAGG 35 3.1724914E-5 82.12143 4 GGGCTGA 35 3.1724914E-5 82.12143 9 TTAGGGC 40 6.151781E-5 71.85625 6 AAAACTG 45 1.1025647E-4 63.872227 145 GAGCGTC 80 6.1118044E-9 62.874218 9 AAGAGCG 90 1.5510523E-8 55.8882 7 TCGGAAG 90 1.5510523E-8 55.8882 3 CGGAAGA 90 1.5510523E-8 55.8882 4 AGAGCGT 90 1.5510523E-8 55.8882 8 ATCGGAA 90 1.5510523E-8 55.8882 2 GGAAGAG 90 1.5510523E-8 55.8882 5 GATCGGA 100 3.5632183E-8 50.299374 1 GAAGAGC 100 3.5632183E-8 50.299374 6 CTCGGTG 35 3.5040102E-6 29.03283 35-39 >>END_MODULE Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751281 spots for SRR5578453.sra Written 751281 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra Read 751265 spots for SRR5578453.sra Written 751265 spots for SRR5578453.sra SRR ids: ['SRR5578453.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_kp5k6vv9 SRR5578453.sra spots: 15025316 blocks: [[1, 751265], [751266, 1502530], [1502531, 2253795], [2253796, 3005060], [3005061, 3756325], [3756326, 4507590], [4507591, 5258855], [5258856, 6010120], [6010121, 6761385], [6761386, 7512650], [7512651, 8263915], [8263916, 9015180], [9015181, 9766445], [9766446, 10517710], [10517711, 11268975], [11268976, 12020240], [12020241, 12771505], [12771506, 13522770], [13522771, 14274035], [14274036, 15025316]] SRR5578453 file size 5069886 SRR5578453 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578453 SRR5578453_1.fastq SRR5578453_2.fastq Input file: SRR5578453_1.fastq Paired file: SRR5578453_2.fastq trimmed: SRR5578453-trimmed-pair1.fastq, SRR5578453-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 18:15:11 2024 >> started Mon Dec 9 18:15:31 2024 >> done (20.484s) 15025316 read pairs processed; of these: 46495 ( 0.31%) short read pairs filtered out after trimming by size control 525235 ( 3.50%) empty read pairs filtered out after trimming by size control 14453586 (96.19%) read pairs available; of these: 8697759 (60.18%) trimmed read pairs available after processing 5755827 (39.82%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 22 0.00% 19 31 0.00% 20 22 0.00% 21 21 0.00% 22 28 0.00% 23 28 0.00% 24 56 0.00% 25 34 0.00% 26 48 0.00% 27 59 0.00% 28 40 0.00% 29 25 0.00% 30 31 0.00% 31 69 0.00% 32 31 0.00% 33 39 0.00% 34 41 0.00% 35 66 0.00% 36 53 0.00% 37 53 0.00% 38 66 0.00% 39 82 0.00% 40 79 0.00% 41 87 0.00% 42 81 0.00% 43 118 0.00% 44 175 0.00% 45 241 0.00% 46 276 0.00% 47 297 0.00% 48 338 0.00% 49 361 0.00% 50 437 0.00% 51 555 0.00% 52 508 0.00% 53 491 0.00% 54 514 0.00% 55 565 0.00% 56 680 0.00% 57 742 0.01% 58 831 0.01% 59 794 0.01% 60 919 0.01% 61 1160 0.01% 62 1186 0.01% 63 1393 0.01% 64 1586 0.01% 65 2188 0.02% 66 3026 0.02% 67 4332 0.03% 68 5493 0.04% 69 13142 0.09% 70 14674 0.10% 71 9238 0.06% 72 7203 0.05% 73 6133 0.04% 74 6090 0.04% 75 6136 0.04% 76 6223 0.04% 77 6644 0.05% 78 7131 0.05% 79 7656 0.05% 80 8222 0.06% 81 8871 0.06% 82 10595 0.07% 83 11971 0.08% 84 14593 0.10% 85 16602 0.11% 86 17797 0.12% 87 19774 0.14% 88 21244 0.15% 89 22423 0.16% 90 23405 0.16% 91 23850 0.17% 92 25402 0.18% 93 27162 0.19% 94 27742 0.19% 95 28928 0.20% 96 30270 0.21% 97 31473 0.22% 98 32544 0.23% 99 34173 0.24% 100 37175 0.26% 101 38222 0.26% 102 39296 0.27% 103 41576 0.29% 104 43880 0.30% 105 46854 0.32% 106 48272 0.33% 107 49535 0.34% 108 51942 0.36% 109 50395 0.35% 110 52228 0.36% 111 53140 0.37% 112 57249 0.40% 113 62995 0.44% 114 66453 0.46% 115 68220 0.47% 116 67367 0.47% 117 66065 0.46% 118 66690 0.46% 119 68359 0.47% 120 71787 0.50% 121 70475 0.49% 122 73569 0.51% 123 75784 0.52% 124 79534 0.55% 125 80324 0.56% 126 84001 0.58% 127 83533 0.58% 128 80916 0.56% 129 86876 0.60% 130 85245 0.59% 131 87445 0.61% 132 89386 0.62% 133 91413 0.63% 134 94709 0.66% 135 95155 0.66% 136 96395 0.67% 137 98299 0.68% 138 104466 0.72% 139 108684 0.75% 140 111577 0.77% 141 112434 0.78% 142 128631 0.89% 143 134600 0.93% 144 144470 1.00% 145 165552 1.15% 146 197216 1.36% 147 246960 1.71% 148 355306 2.46% 149 681651 4.72% 150 3047744 21.09% 151 5755827 39.82% 14453586 reads passed initial QC criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=21.85 fanout-score-rank=1 prefix-density=6.42 prefix-fanout=2.0 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.57 sequence-density-rank=1 fanout-score=21.85 fanout-score-rank=1 prefix-density=6.42 prefix-fanout=2.0 sequence=TTCGTTTTTTTTCTTG criterion=sequence-density sequence-density=0.98 sequence-density-rank=1 fanout-score=4.80 fanout-score-rank=10 prefix-density=4.65 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.14 sequence-density-rank=18 fanout-score=85.72 fanout-score-rank=1 prefix-density=12.22 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR5578453 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 18:17:35 Started mapping on | Dec 09 18:17:35 Finished on | Dec 09 18:48:18 Mapping speed, Million of reads per hour | 28.23 Number of input reads | 14453586 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 4898098 Uniquely mapped reads % | 33.89% Average mapped length | 282.20 Number of splices: Total | 3647709 Number of splices: Annotated (sjdb) | 3395990 Number of splices: GT/AG | 3596780 Number of splices: GC/AG | 44819 Number of splices: AT/AC | 2286 Number of splices: Non-canonical | 3824 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.01% Deletion average length | 1.46 Insertion rate per base | 0.00% Insertion average length | 1.10 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 245110 % of reads mapped to multiple loci | 1.70% Number of reads mapped to too many loci | 57705 % of reads mapped to too many loci | 0.40% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 61.77% % of reads unmapped: other | 2.25% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9318394 9318394 9318394 N_multimapping 245110 245110 245110 N_noFeature 249867 4734517 293966 N_ambiguous 137088 647 18046 UnstrandedReadsAssigned:4511143 PositiveStrandReadsAssigned:162934 NegativeStrandReadsAssigned:4586086 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=133 echo kmer=129 SRR5578453 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578453-trimmed-pair1.fastq SRR5578453-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,453,586 reads, 4,695,050 reads pseudoaligned [quant] estimated average fragment length: 189.698 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,022 rounds 52973 SRR5578453.ke.tsv 35125 SRR5578453.se.tsv 88098 total ==> SRR5578453.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 747.419 11.4472 4.00875 PNS24247 1044 855.302 0 0 PNS24249 1928 1739.3 14.5839 2.1947 PNS24246 1044 855.302 0 0 PNS24248 1044 855.302 0 0 PNS24244 1471 1282.3 63.9689 13.0573 PNS24243 293 124.039 0 0 KQK14069 1603 1414.3 616.382 114.073 KQK14071 474 289.984 5.95084 5.3713 ==> SRR5578453.se.tsv <== BRADI_1g14170v3 618 BRADI_1g53295v3 41 BRADI_1g59795v3 187 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 468 BRADI_1g74790v3 46 BRADI_1g09890v3 0 BRADI_1g77505v3 77 BRADI_1g48960v3 0 SRR5578453 completed mapping pipeline successfully