Starting /dee2/code/volunteer_pipeline.sh SRR5578454 current disk space = 1522914283520 free memory = 1580075096 SRR5578454 SRAfilesize 23a4daaa4ec64af13ae2d246f84e79e7 SRR5578454.sra SRR5578454.sra file validated SRR5578454 is paired end SRR5578454 is conventional basespace SRR5578454 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578454_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.97525 34.0 34.0 34.0 33.0 34.0 2 33.5455 34.0 34.0 34.0 33.0 34.0 3 33.57925 34.0 34.0 34.0 33.0 34.0 4 33.583 34.0 34.0 34.0 33.0 34.0 5 33.608 34.0 34.0 34.0 33.0 34.0 6 37.35325 38.0 38.0 38.0 36.0 38.0 7 37.437 38.0 38.0 38.0 37.0 38.0 8 37.6285 38.0 38.0 38.0 38.0 38.0 9 37.67375 38.0 38.0 38.0 38.0 38.0 10-14 37.630300000000005 38.0 38.0 38.0 38.0 38.0 15-19 37.609500000000004 38.0 38.0 38.0 38.0 38.0 20-24 37.554050000000004 38.0 38.0 38.0 38.0 38.0 25-29 37.38870000000001 38.0 38.0 38.0 38.0 38.0 30-34 37.3355 38.0 38.0 38.0 38.0 38.0 35-39 37.29585 38.0 38.0 38.0 38.0 38.0 40-44 37.11215 38.0 38.0 38.0 37.2 38.0 45-49 37.17915 38.0 38.0 38.0 37.0 38.0 50-54 37.147400000000005 38.0 38.0 38.0 37.0 38.0 55-59 37.070350000000005 38.0 38.0 38.0 37.0 38.0 60-64 37.00395 38.0 38.0 38.0 37.0 38.0 65-69 36.93929999999999 38.0 38.0 38.0 36.4 38.0 70-74 36.812650000000005 38.0 38.0 38.0 36.4 38.0 75-79 36.34895 38.0 38.0 38.0 35.8 38.0 80-84 36.267199999999995 38.0 38.0 38.0 35.6 38.0 85-89 36.1819 38.0 38.0 38.0 34.8 38.0 90-94 36.056799999999996 38.0 38.0 38.0 34.6 38.0 95-99 35.94315000000001 38.0 38.0 38.0 34.0 38.0 100-104 35.8248 38.0 38.0 38.0 34.0 38.0 105-109 35.70205 38.0 38.0 38.0 33.6 38.0 110-114 35.6235 38.0 38.0 38.0 33.4 38.0 115-119 35.43195 38.0 38.0 38.0 32.8 38.0 120-124 35.3507 38.0 37.8 38.0 32.2 38.0 125-129 35.1353 38.0 36.6 38.0 31.0 38.0 130-134 34.9311 38.0 36.0 38.0 30.0 38.0 135-139 34.6896 38.0 36.0 38.0 28.2 38.0 140-144 34.319 38.0 35.4 38.0 26.8 38.0 145-149 33.76475000000001 38.0 35.0 38.0 21.4 38.0 150-151 30.544625 36.5 29.0 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 8.0 7 4.0 8 3.0 9 1.0 10 7.0 11 3.0 12 9.0 13 7.0 14 3.0 15 10.0 16 6.0 17 4.0 18 17.0 19 42.0 20 5.0 21 2.0 22 5.0 23 6.0 24 10.0 25 9.0 26 10.0 27 9.0 28 24.0 29 19.0 30 26.0 31 33.0 32 39.0 33 63.0 34 79.0 35 150.0 36 518.0 37 2868.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 48.07447079826575 10.96659015557256 10.864575363427697 30.094363682734 2 24.474999999999998 12.975 30.875000000000004 31.674999999999997 3 23.48087021755439 15.853963490872719 26.78169542385596 33.88347086771693 4 26.974999999999998 22.2 24.7 26.125 5 27.375 24.95 28.275 19.400000000000002 6 23.125 28.975 29.5 18.4 7 16.625 24.4 41.65 17.325 8 19.575 24.15 34.9 21.375 9 21.275 22.2 35.475 21.05 10-14 23.72 27.705000000000002 26.905 21.67 15-19 22.195 25.790000000000003 28.01 24.005000000000003 20-24 22.725 25.674999999999997 28.54 23.06 25-29 21.68 27.205000000000002 28.92 22.195 30-34 21.096054802740134 27.91639581979099 27.2163608180409 23.771188559427973 35-39 23.36616830841542 26.196309815490775 27.336366818340917 23.101155057752887 40-44 24.581229061453072 25.83629181459073 26.691334566728337 22.89114455722786 45-49 24.055 26.44 28.494999999999997 21.01 50-54 24.895 26.009999999999998 26.419999999999998 22.675 55-59 23.695 26.275 27.384999999999998 22.645 60-64 21.93 27.500000000000004 26.939999999999998 23.630000000000003 65-69 21.44 29.345 26.029999999999998 23.185 70-74 22.645 29.09 25.05 23.215 75-79 22.86 26.095000000000002 26.595000000000002 24.45 80-84 23.405 28.095 25.745 22.755 85-89 23.79 26.705000000000002 26.529999999999998 22.975 90-94 23.87 25.795 26.845000000000002 23.49 95-99 22.28 25.765 28.46 23.494999999999997 100-104 22.705000000000002 27.395000000000003 26.26 23.64 105-109 23.815 26.985 26.0 23.200000000000003 110-114 21.87 27.35 26.174999999999997 24.605 115-119 22.075 27.805000000000003 26.590000000000003 23.53 120-124 23.35350302545382 26.60399059858979 24.72370855628344 25.31879781967295 125-129 22.64566141535384 27.776944236059016 24.59114778694674 24.98624656164041 130-134 23.714742948589716 27.030406081216242 25.445089017803564 23.809761952390478 135-139 22.33611680584029 28.40642032101605 26.776338816940846 22.481124056202813 140-144 23.095 27.88 25.505 23.52 145-149 22.855 29.220000000000002 23.415 24.51 150-151 22.473736868434216 27.613806903451728 24.524762381190595 25.387693846923458 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 2.0 2 1.0 3 1.0 4 0.5 5 1.5 6 4.0 7 4.0 8 1.5 9 2.5 10 3.5 11 2.0 12 1.0 13 1.5 14 2.5 15 2.5 16 2.0 17 1.5 18 2.5 19 3.0 20 1.5 21 1.5 22 2.0 23 2.5 24 2.5 25 2.0 26 1.5 27 2.5 28 5.5 29 21.0 30 35.5 31 50.5 32 57.5 33 62.0 34 85.0 35 97.5 36 124.0 37 168.5 38 172.5 39 143.0 40 148.5 41 136.5 42 101.0 43 90.0 44 101.0 45 108.5 46 115.0 47 138.0 48 142.0 49 149.5 50 157.0 51 153.0 52 172.5 53 184.0 54 155.5 55 137.0 56 119.0 57 89.0 58 73.0 59 62.5 60 53.0 61 43.0 62 31.5 63 36.5 64 42.0 65 28.5 66 16.5 67 16.5 68 14.5 69 11.0 70 12.5 71 15.5 72 15.5 73 12.0 74 9.5 75 6.5 76 6.0 77 5.0 78 4.5 79 3.5 80 1.5 81 1.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.975 2 0.0 3 0.025 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.005 35-39 0.005 40-44 0.005 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.015 125-129 0.025 130-134 0.02 135-139 0.005 140-144 0.0 145-149 0.0 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.075 #Duplication Level Percentage of deduplicated Percentage of total 1 85.5434400281393 60.8 2 7.35138937741822 10.45 3 3.165670066830813 6.75 4 1.1959198030249736 3.4000000000000004 5 0.9145269081955681 3.25 6 0.38691523039043263 1.6500000000000001 7 0.35174111853675694 1.7500000000000002 8 0.2110446711220542 1.2 9 0.14069644741470277 0.8999999999999999 >10 0.6683081252198383 6.9750000000000005 >50 0.07034822370735139 2.875 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACATCTCGTATGC 59 1.4749999999999999 TruSeq Adapter, Index 7 (97% over 36bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 56 1.4000000000000001 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 24 0.6 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 24 0.6 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 20 0.5 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 19 0.475 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 18 0.44999999999999996 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 17 0.42500000000000004 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 16 0.4 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 15 0.375 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 14 0.35000000000000003 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 13 0.325 No Hit CAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCA 12 0.3 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 12 0.3 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 12 0.3 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 12 0.3 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 11 0.27499999999999997 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 10 0.25 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 10 0.25 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 10 0.25 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 10 0.25 No Hit GCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTG 9 0.22499999999999998 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 9 0.22499999999999998 No Hit GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA 9 0.22499999999999998 No Hit GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC 9 0.22499999999999998 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 8 0.2 No Hit CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA 8 0.2 No Hit GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT 8 0.2 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 8 0.2 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 8 0.2 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 8 0.2 No Hit GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA 7 0.17500000000000002 No Hit GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT 7 0.17500000000000002 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 7 0.17500000000000002 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 7 0.17500000000000002 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 7 0.17500000000000002 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 7 0.17500000000000002 No Hit GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG 7 0.17500000000000002 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 7 0.17500000000000002 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 7 0.17500000000000002 No Hit TGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATC 7 0.17500000000000002 No Hit CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT 6 0.15 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 6 0.15 No Hit TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA 6 0.15 No Hit GGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATT 6 0.15 No Hit GGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATG 6 0.15 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 6 0.15 No Hit GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG 6 0.15 No Hit GCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAAT 6 0.15 No Hit TGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATAT 6 0.15 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 6 0.15 No Hit GCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAAC 6 0.15 No Hit CCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAA 5 0.125 No Hit GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA 5 0.125 No Hit CAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTAT 5 0.125 No Hit GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCC 5 0.125 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 5 0.125 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit GCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACATATGCAGAT 5 0.125 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5 0.125 No Hit TGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT 5 0.125 No Hit CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT 5 0.125 No Hit GTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGG 5 0.125 No Hit CTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGC 5 0.125 No Hit CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA 5 0.125 No Hit CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT 5 0.125 No Hit ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 5 0.125 No Hit CACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG 5 0.125 No Hit GAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTA 5 0.125 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 5 0.125 No Hit ATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGAT 5 0.125 No Hit GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT 5 0.125 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 5 0.125 No Hit CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA 5 0.125 No Hit ATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGC 5 0.125 No Hit GCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTT 5 0.125 No Hit AGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAA 5 0.125 No Hit CCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.23750000000000002 0.0 0.0 0.0 0.0 86-87 0.3 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.6625 0.0 0.0 0.0 0.0 94-95 0.8125 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.0750000000000002 0.0 0.0 0.0 0.0 100-101 1.25 0.0 0.0 0.0 0.0 102-103 1.525 0.0 0.0 0.0 0.0 104-105 1.85 0.0 0.0 0.0 0.0 106-107 2.1875 0.0 0.0 0.0 0.0 108-109 2.4875 0.0 0.0 0.0 0.0 110-111 2.95 0.0 0.0 0.0 0.0 112-113 3.275 0.0 0.0 0.0 0.0 114-115 3.7 0.0 0.0 0.0 0.0 116-117 4.075 0.0 0.0 0.0 0.0 118-119 4.4625 0.0 0.0 0.0 0.0 120-121 4.8375 0.0 0.0 0.0 0.0 122-123 5.275 0.0 0.0 0.0 0.0 124-125 5.8875 0.0 0.0 0.0 0.0 126-127 6.5625 0.0 0.0 0.0 0.0 128-129 7.45 0.0 0.0 0.0 0.0 130-131 8.0875 0.0 0.0 0.0 0.0 132-133 8.662500000000001 0.0 0.0 0.0 0.0 134-135 9.175 0.0 0.0 0.0 0.0 136-137 9.8625 0.0 0.0 0.0 0.0 138-139 10.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTCGTT 10 0.006830828 145.0 7 TCGTTCT 10 0.006830828 145.0 9 CTCGTTC 10 0.006830828 145.0 8 AGGTGGC 40 2.9585467E-4 21.75 105-109 TAGGTGG 40 2.9585467E-4 21.75 105-109 GATGGGA 45 6.5511256E-4 19.333332 85-89 TATATAG 45 6.5511256E-4 19.333332 100-104 CTCTCAA 45 6.5511256E-4 19.333332 75-79 AACCAAC 45 6.5511256E-4 19.333332 65-69 AGATGGG 45 6.5511256E-4 19.333332 85-89 AAGAAGA 45 6.5511256E-4 19.333332 80-84 GGCTATC 45 6.5511256E-4 19.333332 110-114 TCTCAAA 45 6.5511256E-4 19.333332 75-79 ACAACTC 45 6.5511256E-4 19.333332 70-74 AAAGAAG 55 1.1668232E-4 18.454546 80-84 TATAGGT 40 0.0076550315 18.125 100-104 GGATGAG 50 0.0013298223 17.4 55-59 TATACTA 50 0.0013298223 17.4 95-99 CTATACT 50 0.0013298223 17.4 95-99 GAAGCTA 50 0.0013298223 17.4 90-94 >>END_MODULE SRR5578454 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578454_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.9665 33.0 33.0 34.0 32.0 34.0 2 33.03625 34.0 33.0 34.0 32.0 34.0 3 32.96375 34.0 33.0 34.0 33.0 34.0 4 32.92675 34.0 33.0 34.0 33.0 34.0 5 32.9325 34.0 33.0 34.0 33.0 34.0 6 37.104 38.0 38.0 38.0 37.0 38.0 7 37.11575 38.0 38.0 38.0 37.0 38.0 8 37.07 38.0 38.0 38.0 37.0 38.0 9 37.0275 38.0 38.0 38.0 37.0 38.0 10-14 37.03415 38.0 38.0 38.0 37.0 38.0 15-19 37.0531 38.0 38.0 38.0 37.0 38.0 20-24 37.02094999999999 38.0 38.0 38.0 37.0 38.0 25-29 37.0146 38.0 38.0 38.0 37.0 38.0 30-34 36.99165 38.0 38.0 38.0 37.0 38.0 35-39 36.97735 38.0 38.0 38.0 37.0 38.0 40-44 36.96945000000001 38.0 38.0 38.0 37.0 38.0 45-49 36.8972 38.0 38.0 38.0 36.8 38.0 50-54 36.81025 38.0 38.0 38.0 36.4 38.0 55-59 36.85875 38.0 38.0 38.0 36.2 38.0 60-64 36.863099999999996 38.0 38.0 38.0 36.2 38.0 65-69 36.624700000000004 38.0 38.0 38.0 36.0 38.0 70-74 36.1616 38.0 38.0 38.0 35.0 38.0 75-79 36.1611 38.0 38.0 38.0 34.6 38.0 80-84 36.040800000000004 38.0 38.0 38.0 34.2 38.0 85-89 35.9103 38.0 38.0 38.0 34.0 38.0 90-94 35.9122 38.0 38.0 38.0 34.0 38.0 95-99 35.708650000000006 38.0 38.0 38.0 33.4 38.0 100-104 35.5116 38.0 38.0 38.0 33.0 38.0 105-109 35.32685 38.0 38.0 38.0 32.2 38.0 110-114 35.17355 38.0 37.8 38.0 30.8 38.0 115-119 34.95875 38.0 37.0 38.0 30.0 38.0 120-124 34.71345000000001 38.0 36.2 38.0 28.2 38.0 125-129 34.3957 38.0 35.8 38.0 25.6 38.0 130-134 33.9852 38.0 35.2 38.0 23.0 38.0 135-139 33.5121 38.0 33.8 38.0 20.6 38.0 140-144 32.826150000000005 38.0 33.0 38.0 13.0 38.0 145-149 31.646299999999997 38.0 33.0 38.0 6.0 38.0 150-151 26.35925 33.0 16.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 6.0 4 2.0 5 2.0 6 1.0 7 4.0 8 1.0 9 0.0 10 5.0 11 3.0 12 4.0 13 7.0 14 9.0 15 11.0 16 11.0 17 43.0 18 10.0 19 13.0 20 10.0 21 8.0 22 14.0 23 9.0 24 15.0 25 11.0 26 13.0 27 27.0 28 28.0 29 32.0 30 42.0 31 52.0 32 53.0 33 74.0 34 115.0 35 234.0 36 599.0 37 2520.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.1 19.8 12.925 27.175 2 26.375 26.25 24.9 22.475 3 22.2 25.5 29.825000000000003 22.475 4 26.174999999999997 30.275000000000002 21.675 21.875 5 27.275 32.175 21.95 18.6 6 23.5 33.0 22.725 20.775 7 21.75 22.400000000000002 33.825 22.025 8 22.400000000000002 26.424999999999997 25.3 25.874999999999996 9 24.474999999999998 25.525 26.25 23.75 10-14 25.465 26.935 23.175 24.425 15-19 25.415 26.345000000000002 25.014999999999997 23.225 20-24 25.06 26.905 24.32 23.715 25-29 26.325 26.424999999999997 24.085 23.165 30-34 26.875 26.06 24.915000000000003 22.15 35-39 25.915 25.395 25.11 23.580000000000002 40-44 26.340000000000003 24.959999999999997 25.285000000000004 23.415 45-49 25.52 24.6 26.365 23.515 50-54 24.705 24.245 27.045 24.005000000000003 55-59 24.325 25.580000000000002 27.855 22.24 60-64 22.96 25.965 27.905 23.169999999999998 65-69 22.869999999999997 27.54 26.805 22.785 70-74 22.765 27.35 26.290000000000003 23.595 75-79 21.695 28.99 25.215 24.099999999999998 80-84 23.145 27.625 26.055 23.175 85-89 22.96 27.85 26.47 22.720000000000002 90-94 23.84 27.700000000000003 26.5 21.959999999999997 95-99 23.195 28.685 26.400000000000002 21.72 100-104 23.11 28.720000000000002 25.885 22.285 105-109 23.425 29.45 24.855 22.27 110-114 22.645 29.49 24.565 23.3 115-119 23.73 30.15 23.75 22.37 120-124 23.605 29.705 24.29 22.400000000000002 125-129 23.95 30.130000000000003 23.79 22.13 130-134 24.5 28.16 25.03 22.31 135-139 23.655 29.235 25.259999999999998 21.85 140-144 25.674999999999997 27.779999999999998 25.430000000000003 21.115000000000002 145-149 26.515 27.939999999999998 24.615000000000002 20.93 150-151 27.575 26.3125 24.2375 21.875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 1.0 20 1.0 21 0.5 22 1.5 23 2.0 24 1.0 25 2.0 26 4.0 27 4.5 28 6.5 29 9.0 30 18.5 31 32.5 32 32.0 33 32.0 34 49.5 35 64.0 36 82.0 37 132.0 38 176.5 39 178.5 40 244.5 41 201.0 42 81.5 43 92.0 44 109.0 45 114.0 46 110.0 47 109.5 48 141.5 49 161.0 50 132.0 51 119.0 52 145.0 53 166.0 54 187.5 55 182.0 56 153.0 57 129.5 58 116.0 59 96.5 60 52.5 61 32.0 62 32.5 63 36.5 64 30.5 65 19.5 66 16.5 67 22.0 68 24.5 69 16.0 70 13.0 71 16.0 72 13.5 73 11.5 74 9.5 75 6.5 76 8.5 77 7.0 78 2.0 79 2.5 80 2.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.625 #Duplication Level Percentage of deduplicated Percentage of total 1 83.78986866791745 55.825 2 8.442776735459661 11.25 3 2.9268292682926833 5.8500000000000005 4 1.7260787992495312 4.6 5 0.8255159474671669 2.75 6 0.7129455909943715 2.85 7 0.450281425891182 2.1 8 0.150093808630394 0.8 9 0.075046904315197 0.44999999999999996 >10 0.8630393996247656 12.125 >50 0.0375234521575985 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 56 1.4000000000000001 Illumina Single End PCR Primer 1 (100% over 50bp) CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 39 0.975 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 37 0.9249999999999999 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 35 0.8750000000000001 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 33 0.8250000000000001 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 31 0.775 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 31 0.775 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 29 0.7250000000000001 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 25 0.625 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 24 0.6 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 23 0.575 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 21 0.525 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 19 0.475 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 19 0.475 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 16 0.4 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 15 0.375 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 14 0.35000000000000003 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 13 0.325 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 11 0.27499999999999997 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 10 0.25 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 10 0.25 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 10 0.25 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 10 0.25 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 10 0.25 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 9 0.22499999999999998 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 9 0.22499999999999998 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 8 0.2 No Hit CCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCG 8 0.2 No Hit GACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCA 8 0.2 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 8 0.2 No Hit CCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTA 7 0.17500000000000002 No Hit TGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGC 7 0.17500000000000002 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 7 0.17500000000000002 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 7 0.17500000000000002 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 7 0.17500000000000002 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 7 0.17500000000000002 No Hit GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT 7 0.17500000000000002 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 7 0.17500000000000002 No Hit CTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATC 7 0.17500000000000002 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 7 0.17500000000000002 No Hit CGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGT 7 0.17500000000000002 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 7 0.17500000000000002 No Hit GCACGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCG 6 0.15 No Hit AAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAAC 6 0.15 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 6 0.15 No Hit CAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCG 6 0.15 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 6 0.15 No Hit CGACTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAG 6 0.15 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 6 0.15 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 6 0.15 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 6 0.15 No Hit CCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTG 6 0.15 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 6 0.15 No Hit GAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTT 6 0.15 No Hit TAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAA 6 0.15 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 6 0.15 No Hit GCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAG 6 0.15 No Hit TAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAA 6 0.15 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 6 0.15 No Hit CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG 6 0.15 No Hit CATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCC 6 0.15 No Hit GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC 5 0.125 No Hit CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG 5 0.125 No Hit ACCTAATTGGCGCACAGTACTAGGCATCGTCATCCAATGCGACGAGTCCT 5 0.125 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 5 0.125 No Hit CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG 5 0.125 No Hit GTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAG 5 0.125 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 5 0.125 No Hit GAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCA 5 0.125 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 5 0.125 No Hit GCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAG 5 0.125 No Hit CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC 5 0.125 No Hit GGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGG 5 0.125 No Hit CGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGG 5 0.125 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 5 0.125 No Hit GTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACG 5 0.125 No Hit GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA 5 0.125 No Hit CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA 5 0.125 No Hit GCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACT 5 0.125 No Hit TTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGG 5 0.125 No Hit TTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAG 5 0.125 No Hit GCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACT 5 0.125 No Hit CGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.325 0.0 0.0 0.0 0.0 88-89 0.3625 0.0 0.0 0.0 0.0 90-91 0.4625 0.0 0.0 0.0 0.0 92-93 0.6375 0.0 0.0 0.0 0.0 94-95 0.7875 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.1124999999999998 0.0 0.0 0.0 0.0 100-101 1.2625 0.0 0.0 0.0 0.0 102-103 1.5625 0.0 0.0 0.0 0.0 104-105 1.9249999999999998 0.0 0.0 0.0 0.0 106-107 2.3 0.0 0.0 0.0 0.0 108-109 2.65 0.0 0.0 0.0 0.0 110-111 3.0875000000000004 0.0 0.0 0.0 0.0 112-113 3.45 0.0 0.0 0.0 0.0 114-115 3.9749999999999996 0.0 0.0 0.0 0.0 116-117 4.4 0.0 0.0 0.0 0.0 118-119 4.7875 0.0 0.0 0.0 0.0 120-121 5.1875 0.0 0.0 0.0 0.0 122-123 5.625 0.0 0.0 0.0 0.0 124-125 6.275 0.0 0.0 0.0 0.0 126-127 6.9 0.0 0.0 0.0 0.0 128-129 7.8125 0.0 0.0 0.0 0.0 130-131 8.45 0.0 0.0 0.0 0.0 132-133 8.975 0.0 0.0 0.0 0.0 134-135 9.5125 0.0 0.0 0.0 0.0 136-137 10.2 0.0 0.0 0.0 0.0 138-139 10.8625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTTTG 20 0.00593511 29.0 140-144 CTGGTTT 20 0.00593511 29.0 135-139 CTAAAAA 40 0.0076550315 18.125 110-114 TTTTTGC 50 0.0013298223 17.4 140-144 >>END_MODULE Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635713 spots for SRR5578454.sra Written 635713 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra Read 635694 spots for SRR5578454.sra Written 635694 spots for SRR5578454.sra SRR ids: ['SRR5578454.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_2nlb6ot9 SRR5578454.sra spots: 12713899 blocks: [[1, 635694], [635695, 1271388], [1271389, 1907082], [1907083, 2542776], [2542777, 3178470], [3178471, 3814164], [3814165, 4449858], [4449859, 5085552], [5085553, 5721246], [5721247, 6356940], [6356941, 6992634], [6992635, 7628328], [7628329, 8264022], [8264023, 8899716], [8899717, 9535410], [9535411, 10171104], [10171105, 10806798], [10806799, 11442492], [11442493, 12078186], [12078187, 12713899]] SRR5578454 file size 4286622 SRR5578454 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578454 SRR5578454_1.fastq SRR5578454_2.fastq Input file: SRR5578454_1.fastq Paired file: SRR5578454_2.fastq trimmed: SRR5578454-trimmed-pair1.fastq, SRR5578454-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 18:16:04 2024 >> started Mon Dec 9 18:16:18 2024 >> done (14.680s) 12713899 read pairs processed; of these: 32868 ( 0.26%) short read pairs filtered out after trimming by size control 194603 ( 1.53%) empty read pairs filtered out after trimming by size control 12486428 (98.21%) read pairs available; of these: 6231707 (49.91%) trimmed read pairs available after processing 6254721 (50.09%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 11 0.00% 20 20 0.00% 21 28 0.00% 22 27 0.00% 23 29 0.00% 24 52 0.00% 25 30 0.00% 26 45 0.00% 27 41 0.00% 28 34 0.00% 29 68 0.00% 30 31 0.00% 31 47 0.00% 32 40 0.00% 33 41 0.00% 34 42 0.00% 35 118 0.00% 36 66 0.00% 37 46 0.00% 38 48 0.00% 39 38 0.00% 40 59 0.00% 41 68 0.00% 42 64 0.00% 43 73 0.00% 44 89 0.00% 45 122 0.00% 46 185 0.00% 47 202 0.00% 48 194 0.00% 49 229 0.00% 50 267 0.00% 51 334 0.00% 52 363 0.00% 53 347 0.00% 54 346 0.00% 55 352 0.00% 56 394 0.00% 57 515 0.00% 58 563 0.00% 59 448 0.00% 60 488 0.00% 61 609 0.00% 62 686 0.01% 63 714 0.01% 64 778 0.01% 65 1068 0.01% 66 1669 0.01% 67 2319 0.02% 68 5178 0.04% 69 21687 0.17% 70 27864 0.22% 71 11247 0.09% 72 5293 0.04% 73 3336 0.03% 74 2867 0.02% 75 2751 0.02% 76 2832 0.02% 77 2956 0.02% 78 3206 0.03% 79 3498 0.03% 80 3485 0.03% 81 3892 0.03% 82 4731 0.04% 83 5319 0.04% 84 7731 0.06% 85 10054 0.08% 86 12373 0.10% 87 15562 0.12% 88 16201 0.13% 89 16515 0.13% 90 16647 0.13% 91 14882 0.12% 92 15194 0.12% 93 15365 0.12% 94 14855 0.12% 95 15191 0.12% 96 15222 0.12% 97 15617 0.13% 98 16460 0.13% 99 17482 0.14% 100 19027 0.15% 101 19778 0.16% 102 20102 0.16% 103 21996 0.18% 104 23267 0.19% 105 25430 0.20% 106 26125 0.21% 107 27323 0.22% 108 28163 0.23% 109 28362 0.23% 110 29206 0.23% 111 30708 0.25% 112 32497 0.26% 113 36612 0.29% 114 40366 0.32% 115 42271 0.34% 116 42596 0.34% 117 40815 0.33% 118 40364 0.32% 119 41209 0.33% 120 44545 0.36% 121 43346 0.35% 122 44239 0.35% 123 45640 0.37% 124 47146 0.38% 125 48163 0.39% 126 50400 0.40% 127 51865 0.42% 128 50806 0.41% 129 54178 0.43% 130 53644 0.43% 131 54204 0.43% 132 56369 0.45% 133 59369 0.48% 134 59594 0.48% 135 60877 0.49% 136 62326 0.50% 137 64313 0.52% 138 68618 0.55% 139 71792 0.57% 140 74804 0.60% 141 74637 0.60% 142 86769 0.69% 143 90111 0.72% 144 95814 0.77% 145 109188 0.87% 146 129982 1.04% 147 162779 1.30% 148 231655 1.86% 149 472661 3.79% 150 2635709 21.11% 151 6254721 50.09% 12486428 reads passed initial QC criterion=sequence-density sequence-density=0.73 sequence-density-rank=1 fanout-score=22.12 fanout-score-rank=2 prefix-density=8.30 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.08 sequence-density-rank=26 fanout-score=46.86 fanout-score-rank=1 prefix-density=3.53 prefix-fanout=1.1 sequence=TCGTTTTTTTTTCTT criterion=sequence-density sequence-density=1.33 sequence-density-rank=1 fanout-score=4.74 fanout-score-rank=9 prefix-density=6.26 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.15 sequence-density-rank=14 fanout-score=88.50 fanout-score-rank=1 prefix-density=13.34 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR5578454 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 18:18:27 Started mapping on | Dec 09 18:18:27 Finished on | Dec 09 18:53:48 Mapping speed, Million of reads per hour | 21.19 Number of input reads | 12486428 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 3156292 Uniquely mapped reads % | 25.28% Average mapped length | 287.87 Number of splices: Total | 2117246 Number of splices: Annotated (sjdb) | 1977144 Number of splices: GT/AG | 2090628 Number of splices: GC/AG | 22505 Number of splices: AT/AC | 864 Number of splices: Non-canonical | 3249 Mismatch rate per base, % | 0.11% Deletion rate per base | 0.00% Deletion average length | 1.38 Insertion rate per base | 0.01% Insertion average length | 1.25 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 138678 % of reads mapped to multiple loci | 1.11% Number of reads mapped to too many loci | 41921 % of reads mapped to too many loci | 0.34% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 71.81% % of reads unmapped: other | 1.46% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9199650 9199650 9199650 N_multimapping 138678 138678 138678 N_noFeature 158065 3013830 203959 N_ambiguous 110896 306 14941 UnstrandedReadsAssigned:2887331 PositiveStrandReadsAssigned:142156 NegativeStrandReadsAssigned:2937392 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=147 echo kmer=143 SRR5578454 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578454-trimmed-pair1.fastq SRR5578454-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 12,486,428 reads, 2,984,485 reads pseudoaligned [quant] estimated average fragment length: 207.063 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,005 rounds 52973 SRR5578454.ke.tsv 35125 SRR5578454.se.tsv 88098 total ==> SRR5578454.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 730.171 21.7882 9.84864 PNS24247 1044 837.937 0 0 PNS24249 1928 1721.94 15.0717 2.88884 PNS24246 1044 837.937 0 0 PNS24248 1044 837.937 0 0 PNS24244 1471 1264.94 29.1402 7.60332 PNS24243 293 111.914 0 0 KQK14069 1603 1396.94 187.708 44.3493 KQK14071 474 273.407 2.29185 2.76666 ==> SRR5578454.se.tsv <== BRADI_1g14170v3 189 BRADI_1g53295v3 0 BRADI_1g59795v3 39 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 50 BRADI_1g74790v3 311 BRADI_1g09890v3 1 BRADI_1g77505v3 22 BRADI_1g48960v3 0 SRR5578454 completed mapping pipeline successfully