Starting /dee2/code/volunteer_pipeline.sh SRR5578464 current disk space = 1522604412928 free memory = 1573978008 SRR5578464 SRAfilesize ad44670a2fb3f79371bc62473adc0488 SRR5578464.sra SRR5578464.sra file validated SRR5578464 is paired end SRR5578464 is conventional basespace SRR5578464 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578464_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.3225 34.0 33.0 34.0 33.0 34.0 2 33.49375 34.0 34.0 34.0 33.0 34.0 3 33.53275 34.0 34.0 34.0 33.0 34.0 4 33.4895 34.0 34.0 34.0 33.0 34.0 5 33.542 34.0 34.0 34.0 33.0 34.0 6 37.2705 38.0 38.0 38.0 36.0 38.0 7 37.374 38.0 38.0 38.0 37.0 38.0 8 37.46475 38.0 38.0 38.0 37.0 38.0 9 37.498 38.0 38.0 38.0 37.0 38.0 10-14 37.52119999999999 38.0 38.0 38.0 38.0 38.0 15-19 37.580349999999996 38.0 38.0 38.0 38.0 38.0 20-24 37.5146 38.0 38.0 38.0 38.0 38.0 25-29 37.4653 38.0 38.0 38.0 38.0 38.0 30-34 37.36945 38.0 38.0 38.0 37.6 38.0 35-39 37.34930000000001 38.0 38.0 38.0 37.2 38.0 40-44 37.176050000000004 38.0 38.0 38.0 36.8 38.0 45-49 37.2741 38.0 38.0 38.0 37.0 38.0 50-54 37.297450000000005 38.0 38.0 38.0 37.0 38.0 55-59 37.2252 38.0 38.0 38.0 37.0 38.0 60-64 37.22855 38.0 38.0 38.0 37.0 38.0 65-69 37.18215 38.0 38.0 38.0 37.0 38.0 70-74 37.02915 38.0 38.0 38.0 36.8 38.0 75-79 36.40885 38.0 38.0 38.0 36.0 38.0 80-84 36.35395 38.0 38.0 38.0 36.0 38.0 85-89 36.240249999999996 38.0 38.0 38.0 35.2 38.0 90-94 36.124 38.0 38.0 38.0 34.8 38.0 95-99 36.0899 38.0 38.0 38.0 34.6 38.0 100-104 35.96395 38.0 38.0 38.0 34.4 38.0 105-109 35.935050000000004 38.0 38.0 38.0 34.4 38.0 110-114 35.84005 38.0 38.0 38.0 34.0 38.0 115-119 35.683 38.0 38.0 38.0 33.8 38.0 120-124 35.60039999999999 38.0 38.0 38.0 33.2 38.0 125-129 35.476749999999996 38.0 38.0 38.0 32.8 38.0 130-134 35.2017 38.0 37.8 38.0 31.8 38.0 135-139 35.07125 38.0 37.8 38.0 30.6 38.0 140-144 34.74995 38.0 36.4 38.0 29.0 38.0 145-149 34.27015 38.0 36.0 38.0 26.8 38.0 150-151 30.874 35.5 30.5 38.0 12.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 2.0 8 2.0 9 0.0 10 1.0 11 2.0 12 2.0 13 2.0 14 1.0 15 5.0 16 2.0 17 8.0 18 33.0 19 46.0 20 5.0 21 11.0 22 9.0 23 11.0 24 10.0 25 7.0 26 14.0 27 11.0 28 15.0 29 25.0 30 24.0 31 27.0 32 45.0 33 49.0 34 77.0 35 147.0 36 363.0 37 3040.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 48.303593867805986 11.786881125911034 8.871575772807239 31.037949233475747 2 26.724999999999998 14.075 30.7 28.499999999999996 3 23.225 16.25 26.950000000000003 33.575 4 26.150000000000002 23.474999999999998 24.6 25.775 5 27.85 25.85 25.424999999999997 20.875 6 22.400000000000002 30.55 26.75 20.3 7 15.15 25.575 41.75 17.525 8 19.125 26.224999999999998 32.225 22.425 9 21.175 22.25 36.6 19.975 10-14 23.415 26.424999999999997 26.985 23.175 15-19 22.405 25.650000000000002 28.194999999999997 23.75 20-24 22.715 25.085 28.744999999999997 23.455000000000002 25-29 21.0 25.919999999999998 29.7 23.380000000000003 30-34 20.91 26.865 28.825 23.400000000000002 35-39 21.94 25.695 28.610000000000003 23.755000000000003 40-44 23.544999999999998 25.435000000000002 26.995 24.025 45-49 23.380000000000003 25.915 28.599999999999998 22.105 50-54 24.34 24.959999999999997 27.185 23.515 55-59 22.884999999999998 25.16 28.470000000000002 23.485 60-64 21.44 26.779999999999998 28.055000000000003 23.724999999999998 65-69 20.94 28.315 26.46 24.285 70-74 22.54 28.165000000000003 25.77 23.525 75-79 21.88 26.47 27.1 24.55 80-84 23.125 26.695 26.66 23.52 85-89 23.66 25.740000000000002 27.01 23.59 90-94 23.685000000000002 25.240000000000002 28.005000000000003 23.07 95-99 22.645 25.025 27.994999999999997 24.335 100-104 23.555 27.165 25.929999999999996 23.35 105-109 23.025000000000002 26.685 26.875 23.415 110-114 21.85 26.58 26.32 25.25 115-119 21.77 27.015 26.655 24.560000000000002 120-124 22.985 26.845000000000002 25.61 24.560000000000002 125-129 22.655 27.575 25.064999999999998 24.705 130-134 23.419999999999998 26.44 25.759999999999998 24.38 135-139 22.685 27.905 26.205000000000002 23.205000000000002 140-144 23.115 28.02 25.445 23.419999999999998 145-149 23.335 28.110000000000003 24.884999999999998 23.669999999999998 150-151 22.5625 26.5875 25.45 25.4 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.5 5 1.5 6 1.5 7 1.5 8 0.5 9 0.5 10 1.0 11 1.5 12 0.5 13 0.0 14 0.5 15 0.5 16 1.0 17 2.5 18 1.5 19 1.5 20 1.5 21 2.5 22 3.0 23 0.5 24 1.0 25 2.0 26 3.5 27 4.0 28 5.0 29 21.5 30 35.5 31 50.5 32 53.5 33 57.0 34 76.5 35 86.5 36 112.0 37 148.5 38 147.0 39 113.5 40 133.0 41 139.5 42 100.5 43 91.0 44 113.0 45 134.5 46 158.5 47 180.0 48 188.0 49 198.0 50 193.0 51 169.5 52 154.5 53 162.0 54 142.0 55 118.0 56 112.5 57 94.5 58 75.5 59 65.0 60 55.5 61 44.0 62 39.0 63 35.5 64 27.5 65 20.0 66 12.0 67 7.5 68 10.0 69 12.5 70 13.5 71 10.5 72 7.5 73 8.5 74 7.0 75 5.0 76 5.0 77 5.5 78 4.5 79 1.5 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.525 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.875 #Duplication Level Percentage of deduplicated Percentage of total 1 83.23478260869565 59.824999999999996 2 10.08695652173913 14.499999999999998 3 3.0608695652173914 6.6000000000000005 4 1.3565217391304347 3.9 5 0.8347826086956521 3.0 6 0.3826086956521739 1.6500000000000001 7 0.17391304347826086 0.8750000000000001 8 0.2782608695652174 1.6 9 0.06956521739130435 0.44999999999999996 >10 0.4869565217391304 5.4 >50 0.034782608695652174 2.1999999999999997 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAATGATCTCGTATGC 88 2.1999999999999997 TruSeq Adapter, Index 2 (97% over 36bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 46 1.15 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 24 0.6 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 16 0.4 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 14 0.35000000000000003 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 14 0.35000000000000003 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 13 0.325 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 13 0.325 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 12 0.3 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 12 0.3 No Hit GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT 11 0.27499999999999997 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 11 0.27499999999999997 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 10 0.25 No Hit GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA 10 0.25 No Hit GCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAAT 10 0.25 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 9 0.22499999999999998 No Hit GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG 9 0.22499999999999998 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 8 0.2 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 8 0.2 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 8 0.2 No Hit CACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCT 8 0.2 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 8 0.2 No Hit GGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTT 8 0.2 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 8 0.2 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 8 0.2 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 7 0.17500000000000002 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 7 0.17500000000000002 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 7 0.17500000000000002 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 7 0.17500000000000002 No Hit GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA 7 0.17500000000000002 No Hit CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT 6 0.15 No Hit CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT 6 0.15 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 6 0.15 No Hit CTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCT 6 0.15 No Hit ATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTG 6 0.15 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 6 0.15 No Hit CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA 6 0.15 No Hit AGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTT 6 0.15 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 6 0.15 No Hit GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC 6 0.15 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 6 0.15 No Hit GGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGT 5 0.125 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 5 0.125 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 5 0.125 No Hit CTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTT 5 0.125 No Hit CAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGAT 5 0.125 No Hit GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT 5 0.125 No Hit GGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATA 5 0.125 No Hit CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG 5 0.125 No Hit GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA 5 0.125 No Hit GTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTG 5 0.125 No Hit GCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAAT 5 0.125 No Hit GTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTAC 5 0.125 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 5 0.125 No Hit GTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTAT 5 0.125 No Hit GCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAA 5 0.125 No Hit CCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCT 5 0.125 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 5 0.125 No Hit GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA 5 0.125 No Hit AGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTATATT 5 0.125 No Hit CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT 5 0.125 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 5 0.125 No Hit CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC 5 0.125 No Hit GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC 5 0.125 No Hit CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.037500000000000006 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.25 0.0 0.0 0.0 0.0 74-75 0.3 0.0 0.0 0.0 0.0 76-77 0.38749999999999996 0.0 0.0 0.0 0.0 78-79 0.4625 0.0 0.0 0.0 0.0 80-81 0.525 0.0 0.0 0.0 0.0 82-83 0.7375 0.0 0.0 0.0 0.0 84-85 0.925 0.0 0.0 0.0 0.0 86-87 0.9875 0.0 0.0 0.0 0.0 88-89 1.1375 0.0 0.0 0.0 0.0 90-91 1.5 0.0 0.0 0.0 0.0 92-93 1.75 0.0 0.0 0.0 0.0 94-95 2.125 0.0 0.0 0.0 0.0 96-97 2.4000000000000004 0.0 0.0 0.0 0.0 98-99 2.7125 0.0 0.0 0.0 0.0 100-101 3.15 0.0 0.0 0.0 0.0 102-103 3.75 0.0 0.0 0.0 0.0 104-105 4.2875 0.0 0.0 0.0 0.0 106-107 4.875 0.0 0.0 0.0 0.0 108-109 5.4625 0.0 0.0 0.0 0.0 110-111 5.925000000000001 0.0 0.0 0.0 0.0 112-113 6.65 0.0 0.0 0.0 0.0 114-115 7.300000000000001 0.0 0.0 0.0 0.0 116-117 8.0625 0.0 0.0 0.0 0.0 118-119 8.675 0.0 0.0 0.0 0.0 120-121 9.475000000000001 0.0 0.0 0.0 0.0 122-123 10.4 0.0 0.0 0.0 0.0 124-125 11.3375 0.0 0.0 0.0 0.0 126-127 12.175 0.0 0.0 0.0 0.0 128-129 13.325 0.0 0.0 0.0 0.0 130-131 14.275 0.0 0.0 0.0 0.0 132-133 15.212499999999999 0.0 0.0 0.0 0.0 134-135 16.35 0.0 0.0 0.0 0.0 136-137 17.1625 0.0 0.0 0.0 0.0 138-139 18.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCACA 10 0.006830828 145.0 5 TTCACAC 10 0.006830828 145.0 6 CACACAA 10 0.006830828 145.0 8 TCAATGC 10 0.006830828 145.0 145 CTTGGGG 10 0.006830828 145.0 6 ACTTCAC 10 0.006830828 145.0 4 CCACTTC 10 0.006830828 145.0 2 ACACAAG 10 0.006830828 145.0 9 TGGGGTG 10 0.006830828 145.0 8 TTGGGGT 10 0.006830828 145.0 7 TCTTGGG 10 0.006830828 145.0 5 GCCACTT 10 0.006830828 145.0 1 ATGCATC 10 0.006830828 145.0 145 GGGGTGG 10 0.006830828 145.0 9 CTCATCT 10 0.006830828 145.0 1 TCACACA 10 0.006830828 145.0 7 >>END_MODULE SRR5578464 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578464_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17175 33.0 33.0 34.0 31.0 34.0 2 32.221 33.0 33.0 34.0 31.0 34.0 3 32.12475 33.0 33.0 34.0 29.0 34.0 4 32.20575 33.0 33.0 34.0 31.0 34.0 5 32.1115 33.0 33.0 34.0 30.0 34.0 6 36.38175 38.0 38.0 38.0 34.0 38.0 7 36.25025 38.0 38.0 38.0 34.0 38.0 8 36.19 38.0 38.0 38.0 33.0 38.0 9 36.28175 38.0 38.0 38.0 34.0 38.0 10-14 36.2236 38.0 38.0 38.0 33.8 38.0 15-19 36.046949999999995 38.0 38.0 38.0 33.0 38.0 20-24 35.8949 38.0 38.0 38.0 32.8 38.0 25-29 35.772149999999996 38.0 37.2 38.0 31.4 38.0 30-34 35.49605 38.0 37.0 38.0 29.4 38.0 35-39 35.302049999999994 38.0 37.0 38.0 29.0 38.0 40-44 35.11725 38.0 36.8 38.0 28.4 38.0 45-49 34.84685 38.0 36.0 38.0 27.4 38.0 50-54 34.660250000000005 38.0 36.0 38.0 27.0 38.0 55-59 34.35905 38.0 35.2 38.0 25.4 38.0 60-64 34.22135 38.0 34.8 38.0 23.0 38.0 65-69 33.67055 38.0 34.2 38.0 17.6 38.0 70-74 33.05175 38.0 33.6 38.0 15.8 38.0 75-79 32.63835 37.8 33.0 38.0 15.2 38.0 80-84 32.03810000000001 37.0 31.0 38.0 15.0 38.0 85-89 31.6518 37.0 29.4 38.0 15.0 38.0 90-94 31.000100000000003 36.6 28.4 38.0 14.4 38.0 95-99 30.07805 36.0 25.6 38.0 13.0 38.0 100-104 29.1621 35.0 23.4 38.0 8.6 38.0 105-109 28.377050000000004 35.0 20.6 38.0 2.0 38.0 110-114 27.06495 34.0 15.0 38.0 2.0 38.0 115-119 25.89995 33.6 14.4 38.0 2.0 38.0 120-124 24.6377 31.4 13.6 37.6 2.0 38.0 125-129 23.3093 29.4 10.8 36.4 2.0 38.0 130-134 21.779000000000003 25.2 2.0 35.8 2.0 38.0 135-139 20.2913 22.6 2.0 35.0 2.0 38.0 140-144 18.435299999999998 15.8 2.0 34.2 2.0 38.0 145-149 15.85155 6.6 2.0 33.6 2.0 38.0 150-151 12.091875 2.0 2.0 28.5 2.0 36.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25.0 3 9.0 4 8.0 5 11.0 6 9.0 7 9.0 8 5.0 9 10.0 10 7.0 11 10.0 12 21.0 13 16.0 14 35.0 15 29.0 16 30.0 17 38.0 18 36.0 19 47.0 20 50.0 21 71.0 22 77.0 23 80.0 24 90.0 25 95.0 26 114.0 27 140.0 28 170.0 29 155.0 30 225.0 31 255.0 32 251.0 33 394.0 34 444.0 35 433.0 36 441.0 37 160.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.425 19.775000000000002 13.0 25.8 2 30.345518277416122 26.86529794692038 22.183274912368553 20.605908863294943 3 24.204460035078927 25.482335254322226 28.564269606614882 21.748935103983964 4 25.288220551378448 32.23057644110276 20.977443609022554 21.503759398496243 5 29.090453520420944 32.07216236532198 20.07015785517414 18.767226259082936 6 24.937468734367183 35.217608804402204 19.70985492746373 20.135067533766886 7 21.46073036518259 24.81240620310155 32.666333166583286 21.060530265132567 8 22.43060765191298 27.60690172543136 24.381095273818453 25.581395348837212 9 26.450000000000003 25.025 25.4 23.125 10-14 25.197638346842787 27.16901831281897 23.136195336735714 24.497148003602522 15-19 25.762050152660294 26.89824315531308 24.610841383452627 22.728865308574004 20-24 26.06127352823388 27.758309971966362 23.237885462555067 22.94253103724469 25-29 25.80838922815097 27.955751326459104 23.130443487836622 23.10541595755331 30-34 26.229918422501374 27.120764726490165 24.278064160952905 22.371252690055552 35-39 24.974974974974977 26.106106106106107 24.654654654654653 24.264264264264266 40-44 26.57454691098428 26.439371182537297 24.727145288875537 22.258936617602885 45-49 25.409302558453913 26.085215040304412 25.38426876282982 23.121213638411856 50-54 24.421864050455504 25.96856542196416 26.148763640004002 23.460806887576332 55-59 22.687224669603523 27.833400080096116 26.762114537444937 22.717260712855428 60-64 22.41362043064597 28.517776664997495 25.98898347521282 23.079619429143715 65-69 22.604385701411836 29.908881546009813 24.747171322719534 22.739561429858817 70-74 22.585326794114703 29.271344209788808 24.447002302071866 23.696326694024624 75-79 22.71839799749687 29.82728410513141 23.994993742177723 23.459324155193993 80-84 23.262567594632486 29.521329861806528 24.123773282595636 23.092329260965354 85-89 22.727272727272727 28.80456547857429 25.470564677613133 22.997597116539847 90-94 24.19677709938945 29.246321689520567 24.892403162846563 21.66449804824342 95-99 23.522932104946925 29.961946725415583 24.25395553775285 22.261165631884637 100-104 23.635453179769655 30.14521782674011 23.805708562844266 22.41362043064597 105-109 24.171422849704616 31.00530689896866 23.480524682086713 21.342745569240012 110-114 23.35854159362949 30.069614864526468 23.173235839134573 23.39860770270947 115-119 24.365329728105753 31.250312953782984 22.612788543387914 21.77156877472335 120-124 24.394273127753305 30.25130156187425 23.317981577893473 22.036443732478975 125-129 25.00625782227785 31.254067584480598 21.92240300375469 21.817271589486857 130-134 25.185259363108354 29.871820548768273 23.03224514320048 21.910674944922892 135-139 24.531985183702073 30.999099008909802 22.805085594153567 21.663830213234558 140-144 26.239679759819868 29.942456842631977 22.922191643732802 20.89567175381536 145-149 26.406124899919938 30.36429143314652 22.44295436349079 20.786629303442755 150-151 27.058823529411764 30.988735919899874 22.06508135168961 19.887359198998748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.0 3 1.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 1.0 20 1.5 21 0.5 22 0.5 23 1.5 24 1.0 25 1.0 26 2.5 27 5.0 28 9.0 29 10.5 30 14.5 31 31.0 32 41.0 33 43.0 34 54.5 35 68.0 36 91.5 37 122.5 38 147.5 39 165.5 40 203.5 41 163.0 42 104.5 43 114.5 44 106.0 45 98.0 46 117.0 47 139.5 48 169.5 49 194.0 50 163.0 51 144.0 52 156.5 53 158.0 54 165.0 55 166.5 56 151.5 57 127.0 58 100.0 59 83.5 60 63.5 61 42.5 62 38.5 63 36.0 64 26.5 65 25.0 66 17.5 67 11.0 68 15.0 69 14.0 70 9.0 71 11.0 72 11.0 73 7.5 74 6.0 75 5.5 76 6.5 77 4.5 78 1.0 79 1.5 80 1.5 81 0.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.15 3 0.22499999999999998 4 0.25 5 0.22499999999999998 6 0.05 7 0.05 8 0.025 9 0.0 10-14 0.06999999999999999 15-19 0.105 20-24 0.12 25-29 0.11 30-34 0.095 35-39 0.1 40-44 0.13 45-49 0.135 50-54 0.11 55-59 0.12 60-64 0.15 65-69 0.13 70-74 0.09 75-79 0.125 80-84 0.13999999999999999 85-89 0.12 90-94 0.09 95-99 0.13999999999999999 100-104 0.15 105-109 0.13 110-114 0.165 115-119 0.145 120-124 0.12 125-129 0.125 130-134 0.13999999999999999 135-139 0.11 140-144 0.075 145-149 0.08 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.825 #Duplication Level Percentage of deduplicated Percentage of total 1 84.44135050469892 60.650000000000006 2 8.840932822833276 12.7 3 3.2718412808910546 7.049999999999999 4 1.3226592412112774 3.8 5 0.4872955099199443 1.7500000000000002 6 0.3132613992342499 1.35 7 0.17403411068569438 0.8750000000000001 8 0.17403411068569438 1.0 9 0.24364775495997215 1.575 >10 0.7309432648799165 9.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 39 0.975 Illumina Single End PCR Primer 1 (100% over 50bp) GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 31 0.775 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 28 0.7000000000000001 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 27 0.675 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 26 0.65 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 22 0.5499999999999999 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 18 0.44999999999999996 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 17 0.42500000000000004 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 17 0.42500000000000004 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 17 0.42500000000000004 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 16 0.4 No Hit TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA 14 0.35000000000000003 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 13 0.325 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 12 0.3 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 12 0.3 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 11 0.27499999999999997 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 10 0.25 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 10 0.25 No Hit GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA 10 0.25 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 10 0.25 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 10 0.25 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 9 0.22499999999999998 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 9 0.22499999999999998 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 9 0.22499999999999998 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 9 0.22499999999999998 No Hit GGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAA 9 0.22499999999999998 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 9 0.22499999999999998 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 9 0.22499999999999998 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 8 0.2 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 8 0.2 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 8 0.2 No Hit GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT 8 0.2 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 8 0.2 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 7 0.17500000000000002 No Hit GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA 7 0.17500000000000002 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 7 0.17500000000000002 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 7 0.17500000000000002 No Hit CATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCC 7 0.17500000000000002 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 6 0.15 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 6 0.15 No Hit GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG 6 0.15 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 6 0.15 No Hit GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC 6 0.15 No Hit GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA 6 0.15 No Hit GAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAA 6 0.15 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 6 0.15 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 6 0.15 No Hit TGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACA 5 0.125 No Hit AAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT 5 0.125 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 5 0.125 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 5 0.125 No Hit ATTTTGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGAC 5 0.125 No Hit CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCC 5 0.125 No Hit GGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTTACT 5 0.125 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 5 0.125 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 5 0.125 No Hit AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC 5 0.125 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 5 0.125 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 5 0.125 No Hit CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG 5 0.125 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.037500000000000006 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.275 0.0 0.0 0.0 0.0 74-75 0.325 0.0 0.0 0.0 0.0 76-77 0.4125 0.0 0.0 0.0 0.0 78-79 0.5 0.0 0.0 0.0 0.0 80-81 0.575 0.0 0.0 0.0 0.0 82-83 0.7875 0.0 0.0 0.0 0.0 84-85 0.8875 0.0 0.0 0.0 0.0 86-87 0.9 0.0 0.0 0.0 0.0 88-89 0.9875 0.0 0.0 0.0 0.0 90-91 1.2625 0.0 0.0 0.0 0.0 92-93 1.475 0.0 0.0 0.0 0.0 94-95 1.75 0.0 0.0 0.0 0.0 96-97 1.975 0.0 0.0 0.0 0.0 98-99 2.2125 0.0 0.0 0.0 0.0 100-101 2.525 0.0 0.0 0.0 0.0 102-103 2.9375 0.0 0.0 0.0 0.0 104-105 3.3125 0.0 0.0 0.0 0.0 106-107 3.8125 0.0 0.0 0.0 0.0 108-109 4.137499999999999 0.0 0.0 0.0 0.0 110-111 4.4 0.0 0.0 0.0 0.0 112-113 4.825 0.0 0.0 0.0 0.0 114-115 5.3 0.0 0.0 0.0 0.0 116-117 5.824999999999999 0.0 0.0 0.0 0.0 118-119 6.25 0.0 0.0 0.0 0.0 120-121 6.75 0.0 0.0 0.0 0.0 122-123 7.2375 0.0 0.0 0.0 0.0 124-125 7.75 0.0 0.0 0.0 0.0 126-127 8.2 0.0 0.0 0.0 0.0 128-129 8.875 0.0 0.0 0.0 0.0 130-131 9.412500000000001 0.0 0.0 0.0 0.0 132-133 9.912500000000001 0.0 0.0 0.0 0.0 134-135 10.6125 0.0 0.0 0.0 0.0 136-137 11.0625 0.0 0.0 0.0 0.0 138-139 11.5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACAAT 10 0.006830828 145.0 1 AACAAAG 10 0.006830828 145.0 9 TAAACAA 10 0.006830828 145.0 7 TCGTGAG 10 0.006830828 145.0 2 GACAATA 10 0.006830828 145.0 2 ATAAACA 10 0.006830828 145.0 6 AAAATCA 10 0.006830828 145.0 145 >>END_MODULE Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra Read 782722 spots for SRR5578464.sra Written 782722 spots for SRR5578464.sra Read 782713 spots for SRR5578464.sra Written 782713 spots for SRR5578464.sra SRR ids: ['SRR5578464.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_69jka07t SRR5578464.sra spots: 15654269 blocks: [[1, 782713], [782714, 1565426], [1565427, 2348139], [2348140, 3130852], [3130853, 3913565], [3913566, 4696278], [4696279, 5478991], [5478992, 6261704], [6261705, 7044417], [7044418, 7827130], [7827131, 8609843], [8609844, 9392556], [9392557, 10175269], [10175270, 10957982], [10957983, 11740695], [11740696, 12523408], [12523409, 13306121], [13306122, 14088834], [14088835, 14871547], [14871548, 15654269]] SRR5578464 file size 5283017 SRR5578464 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578464 SRR5578464_1.fastq SRR5578464_2.fastq Input file: SRR5578464_1.fastq Paired file: SRR5578464_2.fastq trimmed: SRR5578464-trimmed-pair1.fastq, SRR5578464-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 18:54:15 2024 >> started Mon Dec 9 18:54:33 2024 >> done (18.119s) 15654269 read pairs processed; of these: 40640 ( 0.26%) short read pairs filtered out after trimming by size control 450918 ( 2.88%) empty read pairs filtered out after trimming by size control 15162711 (96.86%) read pairs available; of these: 8754420 (57.74%) trimmed read pairs available after processing 6408291 (42.26%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 20 0.00% 20 21 0.00% 21 24 0.00% 22 22 0.00% 23 29 0.00% 24 39 0.00% 25 44 0.00% 26 29 0.00% 27 37 0.00% 28 49 0.00% 29 41 0.00% 30 52 0.00% 31 54 0.00% 32 45 0.00% 33 45 0.00% 34 40 0.00% 35 71 0.00% 36 58 0.00% 37 62 0.00% 38 42 0.00% 39 71 0.00% 40 66 0.00% 41 91 0.00% 42 114 0.00% 43 121 0.00% 44 175 0.00% 45 259 0.00% 46 251 0.00% 47 308 0.00% 48 338 0.00% 49 396 0.00% 50 378 0.00% 51 443 0.00% 52 517 0.00% 53 555 0.00% 54 541 0.00% 55 638 0.00% 56 775 0.01% 57 864 0.01% 58 878 0.01% 59 946 0.01% 60 1071 0.01% 61 1192 0.01% 62 1432 0.01% 63 1539 0.01% 64 1936 0.01% 65 2137 0.01% 66 2970 0.02% 67 3633 0.02% 68 5089 0.03% 69 16967 0.11% 70 30272 0.20% 71 13584 0.09% 72 7645 0.05% 73 6469 0.04% 74 6564 0.04% 75 7096 0.05% 76 7359 0.05% 77 7964 0.05% 78 8782 0.06% 79 10215 0.07% 80 10502 0.07% 81 11691 0.08% 82 13763 0.09% 83 15198 0.10% 84 18211 0.12% 85 21097 0.14% 86 21872 0.14% 87 23690 0.16% 88 25552 0.17% 89 26269 0.17% 90 27723 0.18% 91 28667 0.19% 92 30690 0.20% 93 31986 0.21% 94 33782 0.22% 95 35678 0.24% 96 37458 0.25% 97 38557 0.25% 98 39167 0.26% 99 41631 0.27% 100 44999 0.30% 101 46933 0.31% 102 47412 0.31% 103 50085 0.33% 104 52577 0.35% 105 54234 0.36% 106 56846 0.37% 107 57155 0.38% 108 60442 0.40% 109 59653 0.39% 110 62047 0.41% 111 63877 0.42% 112 67174 0.44% 113 73407 0.48% 114 76745 0.51% 115 79610 0.53% 116 78016 0.51% 117 79486 0.52% 118 77541 0.51% 119 76733 0.51% 120 82845 0.55% 121 83049 0.55% 122 86122 0.57% 123 89865 0.59% 124 93413 0.62% 125 95331 0.63% 126 96400 0.64% 127 95466 0.63% 128 93871 0.62% 129 98268 0.65% 130 98737 0.65% 131 99621 0.66% 132 102275 0.67% 133 105577 0.70% 134 108459 0.72% 135 109961 0.73% 136 111352 0.73% 137 113623 0.75% 138 119987 0.79% 139 125392 0.83% 140 128816 0.85% 141 131341 0.87% 142 153653 1.01% 143 157257 1.04% 144 171782 1.13% 145 192724 1.27% 146 220955 1.46% 147 270970 1.79% 148 369837 2.44% 149 613145 4.04% 150 2382693 15.71% 151 6408291 42.26% 15162711 reads passed initial QC criterion=sequence-density sequence-density=0.69 sequence-density-rank=1 fanout-score=2.08 fanout-score-rank=29 prefix-density=0.71 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.46 sequence-density-rank=3 fanout-score=22.30 fanout-score-rank=1 prefix-density=5.27 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=sequence-density sequence-density=0.72 sequence-density-rank=1 fanout-score=5.01 fanout-score-rank=8 prefix-density=3.56 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.11 sequence-density-rank=16 fanout-score=32.32 fanout-score-rank=1 prefix-density=3.34 prefix-fanout=1.1 sequence=GGGCTGAAAGCACAGGTGGCATATGCGAGCAAAGCATTCTCATCAGTCGCAATGATTCAGCTCGCTGAGGAAGAGGGACTTTCTTTAGATGTCGTATCCGGAGGAGAGCTATATACGGCTGTTGCAGCAGGCTTTCCGGCAGAACGCATCCACTTTCATGGAAACAATAAGAGCAGGGAAGAACTGCGGATGGCGCTTGAGCACCGCATCGGCTGCATTGTGGTGGATAATTTCTATGAAATCGCGCTTCTTGAAGACCTATGTAAAGAAACGGGTCACTCCATCGATGTTCTTCTTCGGATCACGCCCGGAGTAGAAGCGCATACGCATGACTACATTACAACGGGCCAGGAAGATTCAAAGTTTGGTTTCGATCTTCATAACGGACAAACTGAACGGGCCATTGAACAAGTATTACAATCGGAACACATTCAGCTGCTGGGTGTCCATTGCCATATCGGCTCGCAAATCTTTGATACGGCCGGTTTTGTGTTAGCAGCGGAAAAAATCT SRR5578464 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 18:56:33 Started mapping on | Dec 09 18:56:34 Finished on | Dec 09 19:30:20 Mapping speed, Million of reads per hour | 26.94 Number of input reads | 15162711 Average input read length | 282 UNIQUE READS: Uniquely mapped reads number | 3434189 Uniquely mapped reads % | 22.65% Average mapped length | 281.95 Number of splices: Total | 2454315 Number of splices: Annotated (sjdb) | 2308374 Number of splices: GT/AG | 2422979 Number of splices: GC/AG | 28163 Number of splices: AT/AC | 770 Number of splices: Non-canonical | 2403 Mismatch rate per base, % | 0.16% Deletion rate per base | 0.00% Deletion average length | 1.41 Insertion rate per base | 0.00% Insertion average length | 1.22 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 695947 % of reads mapped to multiple loci | 4.59% Number of reads mapped to too many loci | 77101 % of reads mapped to too many loci | 0.51% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 69.41% % of reads unmapped: other | 2.84% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 11039789 11039789 11039789 N_multimapping 695947 695947 695947 N_noFeature 590518 3336959 615185 N_ambiguous 90033 1032 17965 UnstrandedReadsAssigned:2753638 PositiveStrandReadsAssigned:96198 NegativeStrandReadsAssigned:2801039 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=137 echo kmer=133 SRR5578464 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578464-trimmed-pair1.fastq SRR5578464-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,162,711 reads, 2,934,115 reads pseudoaligned [quant] estimated average fragment length: 202.265 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,082 rounds 52973 SRR5578464.ke.tsv 35125 SRR5578464.se.tsv 88098 total ==> SRR5578464.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 734.862 0 0 PNS24247 1044 842.735 2.18925 1.04093 PNS24249 1928 1726.74 0 0 PNS24246 1044 842.735 2.18925 1.04093 PNS24248 1044 842.735 2.18925 1.04093 PNS24244 1471 1269.74 33.4323 10.5504 PNS24243 293 119.989 0 0 KQK14069 1603 1401.74 910.463 260.263 KQK14071 474 279.76 22.4915 32.2143 ==> SRR5578464.se.tsv <== BRADI_1g14170v3 963 BRADI_1g53295v3 12 BRADI_1g59795v3 44 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 21 BRADI_1g74790v3 12 BRADI_1g09890v3 0 BRADI_1g77505v3 43 BRADI_1g48960v3 0 SRR5578464 completed mapping pipeline successfully