Starting /dee2/code/volunteer_pipeline.sh SRR5578468 current disk space = 1522629033984 free memory = 1573863328 SRR5578468 SRAfilesize d7284d9ad83c5a903aaa7a1c9a2a7b00 SRR5578468.sra SRR5578468.sra file validated SRR5578468 is paired end SRR5578468 is conventional basespace SRR5578468 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578468_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8785 34.0 34.0 34.0 33.0 34.0 2 33.431 34.0 34.0 34.0 33.0 34.0 3 33.4745 34.0 34.0 34.0 33.0 34.0 4 33.51625 34.0 34.0 34.0 33.0 34.0 5 33.52725 34.0 34.0 34.0 33.0 34.0 6 37.2945 38.0 38.0 38.0 36.0 38.0 7 37.505 38.0 38.0 38.0 37.0 38.0 8 37.55175 38.0 38.0 38.0 38.0 38.0 9 37.6235 38.0 38.0 38.0 38.0 38.0 10-14 37.60074999999999 38.0 38.0 38.0 38.0 38.0 15-19 37.61875 38.0 38.0 38.0 38.0 38.0 20-24 37.5917 38.0 38.0 38.0 38.0 38.0 25-29 37.4679 38.0 38.0 38.0 38.0 38.0 30-34 37.372299999999996 38.0 38.0 38.0 38.0 38.0 35-39 37.33205 38.0 38.0 38.0 37.8 38.0 40-44 37.125099999999996 38.0 38.0 38.0 37.0 38.0 45-49 37.1426 38.0 38.0 38.0 37.0 38.0 50-54 37.1177 38.0 38.0 38.0 37.0 38.0 55-59 37.0958 38.0 38.0 38.0 37.0 38.0 60-64 37.031600000000005 38.0 38.0 38.0 36.4 38.0 65-69 36.946299999999994 38.0 38.0 38.0 36.0 38.0 70-74 36.78815 38.0 38.0 38.0 36.0 38.0 75-79 36.213750000000005 38.0 38.0 38.0 35.2 38.0 80-84 36.067600000000006 38.0 38.0 38.0 34.6 38.0 85-89 36.027 38.0 38.0 38.0 34.6 38.0 90-94 35.8411 38.0 38.0 38.0 34.0 38.0 95-99 35.705600000000004 38.0 38.0 38.0 34.0 38.0 100-104 35.72005 38.0 38.0 38.0 34.0 38.0 105-109 35.5232 38.0 38.0 38.0 33.2 38.0 110-114 35.3706 38.0 37.8 38.0 33.0 38.0 115-119 35.22505 38.0 37.2 38.0 31.2 38.0 120-124 34.9829 38.0 36.6 38.0 30.2 38.0 125-129 34.91055 38.0 36.2 38.0 30.0 38.0 130-134 34.65415 38.0 36.0 38.0 28.0 38.0 135-139 34.470549999999996 38.0 35.8 38.0 27.2 38.0 140-144 33.9815 38.0 35.0 38.0 23.6 38.0 145-149 33.45095 38.0 35.0 38.0 19.8 38.0 150-151 29.859249999999996 35.5 27.0 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 3.0 7 5.0 8 6.0 9 2.0 10 4.0 11 3.0 12 5.0 13 7.0 14 9.0 15 5.0 16 5.0 17 6.0 18 20.0 19 47.0 20 6.0 21 9.0 22 7.0 23 6.0 24 11.0 25 5.0 26 10.0 27 15.0 28 19.0 29 17.0 30 31.0 31 45.0 32 37.0 33 47.0 34 100.0 35 195.0 36 574.0 37 2738.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.421267893660534 11.451942740286299 11.451942740286299 30.67484662576687 2 28.4 13.175 30.599999999999998 27.825 3 24.25 16.650000000000002 26.224999999999998 32.875 4 25.4 25.224999999999998 24.9 24.474999999999998 5 26.019514635976982 25.41906429822367 31.098323742807104 17.463097322992244 6 22.05 32.85 27.425 17.675 7 14.149999999999999 26.150000000000002 44.375 15.325 8 18.6 28.65 34.699999999999996 18.05 9 21.224999999999998 24.15 36.625 18.0 10-14 22.994999999999997 29.575000000000003 28.060000000000002 19.37 15-19 21.59 27.51 28.935 21.965 20-24 21.59 26.31 29.915000000000003 22.185 25-29 20.025000000000002 28.62 30.425 20.93 30-34 20.255000000000003 29.42 28.299999999999997 22.025 35-39 21.87 27.445000000000004 29.095 21.59 40-44 24.060000000000002 26.375 28.13 21.435000000000002 45-49 22.64 29.5 29.29 18.57 50-54 24.89 27.32 27.35 20.44 55-59 21.91 28.299999999999997 28.925 20.865000000000002 60-64 20.71 30.320000000000004 27.224999999999998 21.745 65-69 20.385 31.685000000000002 27.6 20.330000000000002 70-74 21.65 30.79 26.07 21.490000000000002 75-79 21.3 28.275 27.6 22.825 80-84 22.675 28.95 26.805 21.57 85-89 23.275000000000002 28.1 28.084999999999997 20.54 90-94 23.419999999999998 27.74 27.785 21.055 95-99 20.695 27.74 29.275000000000002 22.29 100-104 22.605 29.044999999999998 27.26 21.09 105-109 22.255 30.035 26.705000000000002 21.005 110-114 20.43 28.110000000000003 27.105 24.355 115-119 20.44 29.715000000000003 27.04 22.805 120-124 22.63 28.970000000000002 24.805 23.595 125-129 21.83 30.235 24.67 23.265 130-134 22.465 29.455 25.3 22.78 135-139 20.669999999999998 30.880000000000003 28.055000000000003 20.395 140-144 21.86 30.470000000000002 25.8 21.87 145-149 21.93 31.685000000000002 23.78 22.605 150-151 20.943325409733518 28.975353434254973 26.08532465907669 23.99599649693482 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 1.0 3 3.5 4 3.5 5 1.5 6 1.5 7 1.0 8 1.0 9 2.5 10 1.5 11 0.0 12 0.5 13 1.0 14 1.5 15 2.5 16 2.0 17 1.5 18 1.5 19 1.0 20 1.5 21 2.0 22 1.5 23 1.0 24 1.0 25 1.5 26 4.0 27 6.5 28 7.5 29 23.0 30 43.0 31 72.5 32 87.0 33 94.0 34 114.0 35 130.5 36 182.0 37 264.0 38 268.5 39 197.0 40 182.0 41 168.0 42 112.0 43 84.5 44 85.0 45 88.5 46 105.0 47 132.0 48 143.0 49 143.5 50 120.0 51 115.0 52 151.5 53 161.0 54 136.0 55 101.5 56 76.5 57 71.5 58 64.5 59 55.5 60 46.0 61 32.5 62 22.5 63 19.5 64 17.5 65 9.5 66 4.0 67 1.5 68 3.0 69 2.5 70 2.5 71 2.5 72 1.5 73 1.5 74 1.0 75 1.0 76 1.5 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.1999999999999997 2 0.0 3 0.0 4 0.0 5 0.075 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.25 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59336099585063 46.75 2 11.991701244813278 14.45 3 4.3153526970954355 7.8 4 1.9917012448132778 4.8 5 0.9543568464730291 2.875 6 1.0788381742738589 3.9 7 0.29045643153526973 1.225 8 0.24896265560165973 1.2 9 0.2074688796680498 1.125 >10 1.2448132780082988 12.5 >50 0.08298755186721991 3.375 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC 70 1.7500000000000002 TruSeq Adapter, Index 13 (98% over 50bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 65 1.625 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 35 0.8750000000000001 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 34 0.8500000000000001 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 29 0.7250000000000001 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 28 0.7000000000000001 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 27 0.675 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 25 0.625 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 21 0.525 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 21 0.525 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 20 0.5 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 19 0.475 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 16 0.4 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 16 0.4 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 15 0.375 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 14 0.35000000000000003 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 14 0.35000000000000003 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 14 0.35000000000000003 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 12 0.3 No Hit GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA 12 0.3 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 12 0.3 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 12 0.3 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 11 0.27499999999999997 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 11 0.27499999999999997 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 11 0.27499999999999997 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 11 0.27499999999999997 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 10 0.25 No Hit CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA 10 0.25 No Hit CAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTAT 10 0.25 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 10 0.25 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 10 0.25 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 10 0.25 No Hit CAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCA 9 0.22499999999999998 No Hit GGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAAT 9 0.22499999999999998 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC 9 0.22499999999999998 TruSeq Adapter, Index 13 (98% over 50bp) CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT 9 0.22499999999999998 No Hit GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC 9 0.22499999999999998 No Hit CAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTG 8 0.2 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 8 0.2 No Hit CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC 8 0.2 No Hit CTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGC 8 0.2 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 8 0.2 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 8 0.2 No Hit CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA 7 0.17500000000000002 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 7 0.17500000000000002 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 7 0.17500000000000002 No Hit CCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGG 7 0.17500000000000002 No Hit ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 7 0.17500000000000002 No Hit GCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAA 7 0.17500000000000002 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 7 0.17500000000000002 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 6 0.15 No Hit CCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAG 6 0.15 No Hit GCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTG 6 0.15 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 6 0.15 No Hit TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA 6 0.15 No Hit GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT 6 0.15 No Hit GGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATA 6 0.15 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 6 0.15 No Hit GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT 6 0.15 No Hit GTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAAT 6 0.15 No Hit TGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTT 6 0.15 No Hit GTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTAT 6 0.15 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 6 0.15 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 6 0.15 No Hit GTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATA 6 0.15 No Hit CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA 6 0.15 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 6 0.15 No Hit TGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATC 6 0.15 No Hit TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT 6 0.15 No Hit CGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 6 0.15 No Hit GATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGT 6 0.15 No Hit GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC 6 0.15 No Hit CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT 6 0.15 No Hit CCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCA 6 0.15 No Hit CAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATA 6 0.15 No Hit CCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAG 6 0.15 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 5 0.125 No Hit CCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGT 5 0.125 No Hit TGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATG 5 0.125 No Hit ATCGGTTTGAGGCCCCGAGCTGGAGGGTTCTTATCTGCCTAGTACGCGCG 5 0.125 No Hit CGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACG 5 0.125 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 5 0.125 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 5 0.125 No Hit GATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCA 5 0.125 No Hit CTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAAC 5 0.125 No Hit GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA 5 0.125 No Hit GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG 5 0.125 No Hit GCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACATATGCAGAT 5 0.125 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 5 0.125 No Hit GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG 5 0.125 No Hit GCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAAT 5 0.125 No Hit GTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGA 5 0.125 No Hit CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT 5 0.125 No Hit TGGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCAT 5 0.125 No Hit GCTGACGGCAGGCCCCTAAACCCAAAAGTCGGGTTCTACAGGGTATATGA 5 0.125 No Hit GGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGC 5 0.125 No Hit TTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCAT 5 0.125 No Hit GCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTT 5 0.125 No Hit GTGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.037500000000000006 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.0875 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.16249999999999998 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.25 0.0 0.0 0.0 0.0 74-75 0.275 0.0 0.0 0.0 0.0 76-77 0.36250000000000004 0.0 0.0 0.0 0.0 78-79 0.4125 0.0 0.0 0.0 0.0 80-81 0.5875 0.0 0.0 0.0 0.0 82-83 0.75 0.0 0.0 0.0 0.0 84-85 0.875 0.0 0.0 0.0 0.0 86-87 1.0 0.0 0.0 0.0 0.0 88-89 1.25 0.0 0.0 0.0 0.0 90-91 1.4625 0.0 0.0 0.0 0.0 92-93 1.6875 0.0 0.0 0.0 0.0 94-95 2.0125 0.0 0.0 0.0 0.0 96-97 2.5 0.0 0.0 0.0 0.0 98-99 2.8875 0.0 0.0 0.0 0.0 100-101 3.475 0.0 0.0 0.0 0.0 102-103 3.8499999999999996 0.0 0.0 0.0 0.0 104-105 4.4 0.0 0.0 0.0 0.0 106-107 5.0375 0.0 0.0 0.0 0.0 108-109 5.5375 0.0 0.0 0.0 0.0 110-111 6.1625 0.0 0.0 0.0 0.0 112-113 6.7625 0.0 0.0 0.0 0.0 114-115 7.55 0.0 0.0 0.0 0.0 116-117 8.4375 0.0 0.0 0.0 0.0 118-119 9.3375 0.0 0.0 0.0 0.0 120-121 10.125 0.0 0.0 0.0 0.0 122-123 10.9875 0.0 0.0 0.0 0.0 124-125 11.8875 0.0 0.0 0.0 0.0 126-127 12.774999999999999 0.0 0.0 0.0 0.0 128-129 13.7125 0.0 0.0 0.0 0.0 130-131 14.55 0.0 0.0 0.0 0.0 132-133 15.525 0.0 0.0 0.0 0.0 134-135 16.487499999999997 0.0 0.0 0.0 0.0 136-137 17.4625 0.0 0.0 0.0 0.0 138-139 18.450000000000003 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTTAT 60 5.1524694E-8 74.34615 1 CCTTATG 60 6.1842E-8 72.487495 2 TATGTGA 70 1.7994171E-7 62.13214 5 TGATAGA 70 1.7994171E-7 62.13214 9 CTTATGT 70 1.7994171E-7 62.13214 3 GTGATAG 70 1.7994171E-7 62.13214 8 TTATGTG 70 1.7994171E-7 62.13214 4 TGTGATA 70 1.7994171E-7 62.13214 7 ATGTGAT 75 2.9005605E-7 57.990005 6 TAAACCA 110 2.0336316E-4 32.94886 145 CTGGGGT 70 3.8490973E-5 21.241758 1 ATAGGTG 55 4.8101083E-6 21.087273 100-104 GTGGCTA 55 4.8101083E-6 21.087273 105-109 GGTGGCT 55 4.8101083E-6 21.087273 105-109 TATAGGT 55 4.8101083E-6 21.087273 100-104 ATATAGG 55 4.8101083E-6 21.087273 100-104 TGGCTAT 55 4.8101083E-6 21.087273 105-109 TATCCCT 50 5.6162124E-5 20.2965 115-119 TCCCTAC 50 5.6162124E-5 20.2965 115-119 ATCCCTA 50 5.6162124E-5 20.2965 115-119 >>END_MODULE SRR5578468 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578468_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.93475 33.0 33.0 34.0 32.0 34.0 2 33.0615 34.0 33.0 34.0 32.0 34.0 3 32.9765 34.0 33.0 34.0 32.0 34.0 4 32.897 34.0 33.0 34.0 32.0 34.0 5 32.99575 34.0 33.0 34.0 33.0 34.0 6 37.1345 38.0 38.0 38.0 37.0 38.0 7 37.17225 38.0 38.0 38.0 37.0 38.0 8 37.1605 38.0 38.0 38.0 37.0 38.0 9 37.17475 38.0 38.0 38.0 37.0 38.0 10-14 37.14885 38.0 38.0 38.0 37.0 38.0 15-19 37.094550000000005 38.0 38.0 38.0 37.0 38.0 20-24 37.0988 38.0 38.0 38.0 37.0 38.0 25-29 37.11905 38.0 38.0 38.0 37.0 38.0 30-34 37.150349999999996 38.0 38.0 38.0 37.2 38.0 35-39 37.10435 38.0 38.0 38.0 37.0 38.0 40-44 37.11515000000001 38.0 38.0 38.0 37.0 38.0 45-49 37.08239999999999 38.0 38.0 38.0 37.0 38.0 50-54 37.07639999999999 38.0 38.0 38.0 37.0 38.0 55-59 37.07025 38.0 38.0 38.0 37.0 38.0 60-64 37.1291 38.0 38.0 38.0 37.0 38.0 65-69 36.8214 38.0 38.0 38.0 36.6 38.0 70-74 36.35895 38.0 38.0 38.0 36.0 38.0 75-79 36.266400000000004 38.0 38.0 38.0 36.0 38.0 80-84 36.251050000000006 38.0 38.0 38.0 36.0 38.0 85-89 36.15725 38.0 38.0 38.0 35.6 38.0 90-94 36.0941 38.0 38.0 38.0 35.4 38.0 95-99 36.00605 38.0 38.0 38.0 35.0 38.0 100-104 35.917 38.0 38.0 38.0 34.2 38.0 105-109 35.80585 38.0 38.0 38.0 34.0 38.0 110-114 35.5818 38.0 38.0 38.0 33.6 38.0 115-119 35.415 38.0 38.0 38.0 32.8 38.0 120-124 35.1001 38.0 37.2 38.0 31.0 38.0 125-129 34.9473 38.0 37.0 38.0 30.0 38.0 130-134 34.64875 38.0 36.2 38.0 27.8 38.0 135-139 33.89640000000001 38.0 35.6 38.0 22.2 38.0 140-144 33.207649999999994 38.0 33.4 38.0 15.4 38.0 145-149 31.798099999999998 38.0 33.0 38.0 6.4 38.0 150-151 26.135624999999997 33.0 16.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 3.0 4 3.0 5 2.0 6 1.0 7 0.0 8 0.0 9 2.0 10 1.0 11 7.0 12 10.0 13 5.0 14 7.0 15 6.0 16 10.0 17 61.0 18 8.0 19 4.0 20 7.0 21 6.0 22 6.0 23 9.0 24 15.0 25 13.0 26 10.0 27 17.0 28 20.0 29 23.0 30 40.0 31 37.0 32 63.0 33 84.0 34 115.0 35 170.0 36 491.0 37 2735.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.127691537305964 20.681021532298445 15.798698047070605 28.392588883324986 2 27.37737737737738 27.927927927927925 25.45045045045045 19.244244244244243 3 21.551939924906133 24.455569461827285 31.48936170212766 22.503128911138923 4 24.831038798498124 31.289111389236545 23.35419274092616 20.52565707133917 5 27.97797797797798 31.98198198198198 21.92192192192192 18.11811811811812 6 23.75 33.525 23.75 18.975 7 19.15 23.599999999999998 36.925000000000004 20.325 8 22.175 28.275 26.174999999999997 23.375 9 23.05 25.3 29.825000000000003 21.825 10-14 24.325 27.96 23.849999999999998 23.865 15-19 24.54 27.18 26.314999999999998 21.965 20-24 24.93 27.655 25.624999999999996 21.790000000000003 25-29 25.185000000000002 27.52 25.395 21.9 30-34 25.94 26.384999999999998 26.16 21.515 35-39 24.56991398279656 25.160032006401277 27.425485097019404 22.844568913782755 40-44 25.69 26.345000000000002 26.545 21.42 45-49 23.880000000000003 25.515 28.09 22.515 50-54 22.6 24.349999999999998 30.425 22.625 55-59 21.58 26.085 30.325000000000003 22.009999999999998 60-64 20.356106832049615 26.983094928478547 30.024007202160647 22.636791037311195 65-69 20.004002801961374 29.785850095066547 28.880216151305916 21.329930951666164 70-74 19.66876813769639 29.855899129390572 27.699389572700888 22.775943160212147 75-79 19.373404734497772 30.654121415344576 27.060707672288675 22.911766177868977 80-84 20.301240992794238 29.538630904723778 27.547037630104082 22.613090472377902 85-89 19.89 29.970000000000002 28.749999999999996 21.39 90-94 21.515 29.87 28.32 20.294999999999998 95-99 20.335 31.605 27.884999999999998 20.175 100-104 21.41 31.119999999999997 26.51 20.96 105-109 21.279999999999998 32.910000000000004 24.89 20.919999999999998 110-114 20.271216973578863 31.335068054443553 25.22017614091273 23.173538831064853 115-119 21.858278741811272 32.31984797719658 25.333800070010504 20.488073210981646 120-124 21.875 31.96 25.39 20.775 125-129 23.124624924984996 32.00640128025605 24.219843968793757 20.649129825965193 130-134 22.993449017352603 29.654448167225084 26.864029604440663 20.488073210981646 135-139 22.73 30.775000000000002 26.279999999999998 20.215 140-144 25.715 29.189999999999998 26.479999999999997 18.615000000000002 145-149 25.615 29.304999999999996 25.55 19.53 150-151 26.4125 27.875 26.0125 19.7 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 1.5 19 1.5 20 0.0 21 1.0 22 2.0 23 2.5 24 1.5 25 1.0 26 2.0 27 2.5 28 6.5 29 13.0 30 26.0 31 47.0 32 52.5 33 47.5 34 73.5 35 107.5 36 140.0 37 190.5 38 245.5 39 246.5 40 330.5 41 262.0 42 97.0 43 102.5 44 93.0 45 89.5 46 101.5 47 116.5 48 124.0 49 132.0 50 107.0 51 92.5 52 138.0 53 170.0 54 179.0 55 157.5 56 119.0 57 97.0 58 81.0 59 62.0 60 36.0 61 23.0 62 15.5 63 17.0 64 13.5 65 7.5 66 4.5 67 1.0 68 0.0 69 1.0 70 2.5 71 3.5 72 2.0 73 1.0 74 3.0 75 2.0 76 1.0 77 1.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.1 3 0.125 4 0.125 5 0.1 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.02 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.03 65-69 0.06999999999999999 70-74 0.06999999999999999 75-79 0.095 80-84 0.08 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.08 115-119 0.015 120-124 0.0 125-129 0.02 130-134 0.015 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.725 #Duplication Level Percentage of deduplicated Percentage of total 1 76.65600730927365 41.949999999999996 2 11.87756966651439 13.0 3 4.751027866605756 7.8 4 2.1014161717679305 4.6 5 0.9593421653723162 2.625 6 0.8222932846048424 2.7 7 0.5025125628140703 1.925 8 0.27409776153494747 1.2 9 0.31978072179077205 1.575 >10 1.5989036089538604 17.925 >50 0.13704888076747374 4.7 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 73 1.825 Illumina Single End PCR Primer 1 (100% over 50bp) CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 62 1.55 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 53 1.325 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 45 1.125 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 44 1.0999999999999999 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 40 1.0 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 38 0.95 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 37 0.9249999999999999 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 36 0.8999999999999999 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 33 0.8250000000000001 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 32 0.8 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 29 0.7250000000000001 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 27 0.675 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 27 0.675 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 21 0.525 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 20 0.5 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 20 0.5 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 19 0.475 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 19 0.475 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 16 0.4 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 15 0.375 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 15 0.375 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 14 0.35000000000000003 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 13 0.325 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 13 0.325 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 12 0.3 No Hit GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT 12 0.3 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 12 0.3 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 11 0.27499999999999997 No Hit ACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC 11 0.27499999999999997 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 11 0.27499999999999997 No Hit CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC 11 0.27499999999999997 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 11 0.27499999999999997 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 11 0.27499999999999997 No Hit CACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTAC 11 0.27499999999999997 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 11 0.27499999999999997 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 10 0.25 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 10 0.25 No Hit AAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT 9 0.22499999999999998 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 9 0.22499999999999998 No Hit CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT 9 0.22499999999999998 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 9 0.22499999999999998 No Hit CGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGG 9 0.22499999999999998 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 9 0.22499999999999998 No Hit TTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGG 9 0.22499999999999998 No Hit GACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCA 8 0.2 No Hit CATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAAT 8 0.2 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 8 0.2 No Hit GGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTT 8 0.2 No Hit CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC 8 0.2 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 8 0.2 No Hit CACGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGA 7 0.17500000000000002 No Hit CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT 7 0.17500000000000002 No Hit GGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC 7 0.17500000000000002 No Hit CAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCG 7 0.17500000000000002 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 7 0.17500000000000002 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 7 0.17500000000000002 No Hit GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG 7 0.17500000000000002 No Hit GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA 7 0.17500000000000002 No Hit GGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAA 7 0.17500000000000002 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 7 0.17500000000000002 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 7 0.17500000000000002 No Hit CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA 6 0.15 No Hit CCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTA 6 0.15 No Hit AAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTA 6 0.15 No Hit CGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGT 6 0.15 No Hit CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA 6 0.15 No Hit CCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCG 6 0.15 No Hit CTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTG 6 0.15 No Hit TGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGC 6 0.15 No Hit TTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGAC 6 0.15 No Hit TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA 6 0.15 No Hit GTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAA 6 0.15 No Hit TAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAA 6 0.15 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 6 0.15 No Hit AGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCA 6 0.15 No Hit CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG 6 0.15 No Hit TTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAG 6 0.15 No Hit CATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCC 6 0.15 No Hit TCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTC 6 0.15 No Hit CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA 5 0.125 No Hit CTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAAT 5 0.125 No Hit GCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAG 5 0.125 No Hit TAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTT 5 0.125 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 5 0.125 No Hit GAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATAT 5 0.125 No Hit CTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCT 5 0.125 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 5 0.125 No Hit CAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGC 5 0.125 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 5 0.125 No Hit GTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTC 5 0.125 No Hit TGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAG 5 0.125 No Hit GGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCAC 5 0.125 No Hit GGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATA 5 0.125 No Hit AGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGG 5 0.125 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 5 0.125 No Hit GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA 5 0.125 No Hit GCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTAT 5 0.125 No Hit CTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATC 5 0.125 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 5 0.125 No Hit GGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.037500000000000006 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.0625 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.21250000000000002 0.0 0.0 0.0 0.0 70-71 0.25 0.0 0.0 0.0 0.0 72-73 0.3 0.0 0.0 0.0 0.0 74-75 0.32499999999999996 0.0 0.0 0.0 0.0 76-77 0.4 0.0 0.0 0.0 0.0 78-79 0.4375 0.0 0.0 0.0 0.0 80-81 0.5875 0.0 0.0 0.0 0.0 82-83 0.725 0.0 0.0 0.0 0.0 84-85 0.8625 0.0 0.0 0.0 0.0 86-87 1.0 0.0 0.0 0.0 0.0 88-89 1.25 0.0 0.0 0.0 0.0 90-91 1.4625 0.0 0.0 0.0 0.0 92-93 1.7 0.0 0.0 0.0 0.0 94-95 1.9875 0.0 0.0 0.0 0.0 96-97 2.4875 0.0 0.0 0.0 0.0 98-99 2.8375 0.0 0.0 0.0 0.0 100-101 3.4125 0.0 0.0 0.0 0.0 102-103 3.7375 0.0 0.0 0.0 0.0 104-105 4.300000000000001 0.0 0.0 0.0 0.0 106-107 4.925 0.0 0.0 0.0 0.0 108-109 5.425000000000001 0.0 0.0 0.0 0.0 110-111 6.075 0.0 0.0 0.0 0.0 112-113 6.737500000000001 0.0 0.0 0.0 0.0 114-115 7.525 0.0 0.0 0.0 0.0 116-117 8.3625 0.0 0.0 0.0 0.0 118-119 9.274999999999999 0.0 0.0 0.0 0.0 120-121 10.0625 0.0 0.0 0.0 0.0 122-123 10.9875 0.0 0.0 0.0 0.0 124-125 11.8125 0.0 0.0 0.0 0.0 126-127 12.725000000000001 0.0 0.0 0.0 0.0 128-129 13.6875 0.0 0.0 0.0 0.0 130-131 14.5625 0.0 0.0 0.0 0.0 132-133 15.55 0.0 0.0 0.0 0.0 134-135 16.512500000000003 0.0 0.0 0.0 0.0 136-137 17.5875 0.0 0.0 0.0 0.0 138-139 18.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGGG 15 1.1411342E-4 145.0 5 AATTAGG 15 1.1411342E-4 145.0 4 TAATTAG 15 1.1411342E-4 145.0 3 ATTAATT 25 8.7132835E-4 87.0 1 TTAGGGC 25 8.7132835E-4 87.0 6 TTAATTA 25 8.7132835E-4 87.0 2 TAGGGCT 30 0.0017973486 72.5 7 AGGGCTG 35 0.0033124194 62.14286 8 GGGCTGA 35 0.0033124194 62.14286 9 AAAAAAA 190 0.0021796133 7.631579 60-64 >>END_MODULE Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703137 spots for SRR5578468.sra Written 703137 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra Read 703126 spots for SRR5578468.sra Written 703126 spots for SRR5578468.sra SRR ids: ['SRR5578468.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_nkyyt3vo SRR5578468.sra spots: 14062531 blocks: [[1, 703126], [703127, 1406252], [1406253, 2109378], [2109379, 2812504], [2812505, 3515630], [3515631, 4218756], [4218757, 4921882], [4921883, 5625008], [5625009, 6328134], [6328135, 7031260], [7031261, 7734386], [7734387, 8437512], [8437513, 9140638], [9140639, 9843764], [9843765, 10546890], [10546891, 11250016], [11250017, 11953142], [11953143, 12656268], [12656269, 13359394], [13359395, 14062531]] SRR5578468 file size 4743629 SRR5578468 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578468 SRR5578468_1.fastq SRR5578468_2.fastq Input file: SRR5578468_1.fastq Paired file: SRR5578468_2.fastq trimmed: SRR5578468-trimmed-pair1.fastq, SRR5578468-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 18:59:35 2024 >> started Mon Dec 9 18:59:53 2024 >> done (18.038s) 14062531 read pairs processed; of these: 19706 ( 0.14%) short read pairs filtered out after trimming by size control 299908 ( 2.13%) empty read pairs filtered out after trimming by size control 13742917 (97.73%) read pairs available; of these: 7529491 (54.79%) trimmed read pairs available after processing 6213426 (45.21%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 17 0.00% 20 11 0.00% 21 29 0.00% 22 18 0.00% 23 16 0.00% 24 47 0.00% 25 42 0.00% 26 37 0.00% 27 42 0.00% 28 42 0.00% 29 75 0.00% 30 60 0.00% 31 70 0.00% 32 80 0.00% 33 60 0.00% 34 60 0.00% 35 166 0.00% 36 113 0.00% 37 92 0.00% 38 103 0.00% 39 82 0.00% 40 122 0.00% 41 96 0.00% 42 121 0.00% 43 132 0.00% 44 162 0.00% 45 216 0.00% 46 280 0.00% 47 287 0.00% 48 338 0.00% 49 384 0.00% 50 411 0.00% 51 406 0.00% 52 605 0.00% 53 609 0.00% 54 594 0.00% 55 628 0.00% 56 720 0.01% 57 837 0.01% 58 896 0.01% 59 854 0.01% 60 943 0.01% 61 1197 0.01% 62 1264 0.01% 63 1427 0.01% 64 1678 0.01% 65 2166 0.02% 66 2987 0.02% 67 4231 0.03% 68 7463 0.05% 69 18637 0.14% 70 28411 0.21% 71 24757 0.18% 72 14682 0.11% 73 8732 0.06% 74 7056 0.05% 75 6734 0.05% 76 6738 0.05% 77 7612 0.06% 78 7464 0.05% 79 8589 0.06% 80 8879 0.06% 81 9699 0.07% 82 11859 0.09% 83 12579 0.09% 84 15225 0.11% 85 17442 0.13% 86 18975 0.14% 87 21218 0.15% 88 23117 0.17% 89 24229 0.18% 90 25380 0.18% 91 25340 0.18% 92 26907 0.20% 93 27737 0.20% 94 29593 0.22% 95 30540 0.22% 96 31582 0.23% 97 32556 0.24% 98 33960 0.25% 99 35795 0.26% 100 38661 0.28% 101 39635 0.29% 102 41025 0.30% 103 43685 0.32% 104 46536 0.34% 105 48905 0.36% 106 51243 0.37% 107 52399 0.38% 108 54330 0.40% 109 52313 0.38% 110 53130 0.39% 111 55322 0.40% 112 57934 0.42% 113 65328 0.48% 114 71693 0.52% 115 74642 0.54% 116 73678 0.54% 117 69999 0.51% 118 67208 0.49% 119 65214 0.47% 120 68948 0.50% 121 68225 0.50% 122 72736 0.53% 123 73315 0.53% 124 76377 0.56% 125 74612 0.54% 126 77625 0.56% 127 77699 0.57% 128 71964 0.52% 129 78358 0.57% 130 77116 0.56% 131 76426 0.56% 132 79373 0.58% 133 81906 0.60% 134 83689 0.61% 135 81290 0.59% 136 81428 0.59% 137 80826 0.59% 138 87570 0.64% 139 88931 0.65% 140 93298 0.68% 141 89029 0.65% 142 107364 0.78% 143 106389 0.77% 144 109103 0.79% 145 123305 0.90% 146 142910 1.04% 147 167989 1.22% 148 231484 1.68% 149 454385 3.31% 150 2587594 18.83% 151 6213426 45.21% 13742917 reads passed initial QC criterion=sequence-density sequence-density=0.85 sequence-density-rank=1 fanout-score=21.37 fanout-score-rank=5 prefix-density=9.26 prefix-fanout=2.0 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.10 sequence-density-rank=15 fanout-score=92.67 fanout-score-rank=1 prefix-density=8.65 prefix-fanout=1.0 sequence=GTGATTGTTTTT criterion=sequence-density sequence-density=1.48 sequence-density-rank=1 fanout-score=4.68 fanout-score-rank=13 prefix-density=6.95 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.08 sequence-density-rank=18 fanout-score=212.35 fanout-score-rank=1 prefix-density=17.38 prefix-fanout=1.0 sequence=TATCCATAAAAACTGTAAC SRR5578468 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 19:02:28 Started mapping on | Dec 09 19:02:28 Finished on | Dec 09 19:44:35 Mapping speed, Million of reads per hour | 19.58 Number of input reads | 13742917 Average input read length | 283 UNIQUE READS: Uniquely mapped reads number | 1348204 Uniquely mapped reads % | 9.81% Average mapped length | 271.66 Number of splices: Total | 468073 Number of splices: Annotated (sjdb) | 423899 Number of splices: GT/AG | 460189 Number of splices: GC/AG | 4948 Number of splices: AT/AC | 238 Number of splices: Non-canonical | 2698 Mismatch rate per base, % | 0.11% Deletion rate per base | 0.01% Deletion average length | 1.43 Insertion rate per base | 0.01% Insertion average length | 1.28 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 31103 % of reads mapped to multiple loci | 0.23% Number of reads mapped to too many loci | 35429 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 89.28% % of reads unmapped: other | 0.43% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 12366142 12366142 12366142 N_multimapping 31103 31103 31103 N_noFeature 56522 1283494 74180 N_ambiguous 64640 160 19167 UnstrandedReadsAssigned:1227042 PositiveStrandReadsAssigned:64550 NegativeStrandReadsAssigned:1254857 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=134 echo kmer=129 SRR5578468 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578468-trimmed-pair1.fastq SRR5578468-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 13,742,917 reads, 1,265,896 reads pseudoaligned [quant] estimated average fragment length: 169.013 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 904 rounds 52973 SRR5578468.ke.tsv 35125 SRR5578468.se.tsv 88098 total ==> SRR5578468.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 768.008 0 0 PNS24247 1044 875.987 0 0 PNS24249 1928 1759.99 7.70235 3.30482 PNS24246 1044 875.987 0 0 PNS24248 1044 875.987 0 0 PNS24244 1471 1302.99 7.29765 4.22939 PNS24243 293 136.391 0 0 KQK14069 1603 1434.99 60.7167 31.9517 KQK14071 474 308.213 0 0 ==> SRR5578468.se.tsv <== BRADI_1g14170v3 66 BRADI_1g53295v3 0 BRADI_1g59795v3 32 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 12 BRADI_1g74790v3 40 BRADI_1g09890v3 0 BRADI_1g77505v3 11 BRADI_1g48960v3 0 SRR5578468 completed mapping pipeline successfully