Starting /dee2/code/volunteer_pipeline.sh SRR5578469 current disk space = 1522646339584 free memory = 1573859892 SRR5578469 SRAfilesize 4f45729fcfa112be427f064054c8c804 SRR5578469.sra SRR5578469.sra file validated SRR5578469 is paired end SRR5578469 is conventional basespace SRR5578469 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578469_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5955 34.0 33.0 34.0 33.0 34.0 2 33.38475 34.0 34.0 34.0 33.0 34.0 3 33.47425 34.0 34.0 34.0 33.0 34.0 4 33.53525 34.0 34.0 34.0 33.0 34.0 5 33.54475 34.0 34.0 34.0 33.0 34.0 6 37.206 38.0 38.0 38.0 36.0 38.0 7 37.48325 38.0 38.0 38.0 37.0 38.0 8 37.5635 38.0 38.0 38.0 38.0 38.0 9 37.61875 38.0 38.0 38.0 38.0 38.0 10-14 37.59015 38.0 38.0 38.0 38.0 38.0 15-19 37.5676 38.0 38.0 38.0 38.0 38.0 20-24 37.56655 38.0 38.0 38.0 38.0 38.0 25-29 37.4615 38.0 38.0 38.0 38.0 38.0 30-34 37.42825 38.0 38.0 38.0 38.0 38.0 35-39 37.3266 38.0 38.0 38.0 37.6 38.0 40-44 37.16 38.0 38.0 38.0 36.6 38.0 45-49 37.17555 38.0 38.0 38.0 37.0 38.0 50-54 37.15045 38.0 38.0 38.0 36.6 38.0 55-59 37.14635 38.0 38.0 38.0 36.2 38.0 60-64 37.0933 38.0 38.0 38.0 36.0 38.0 65-69 36.9702 38.0 38.0 38.0 36.0 38.0 70-74 36.691700000000004 38.0 38.0 38.0 34.8 38.0 75-79 36.1503 38.0 38.0 38.0 34.2 38.0 80-84 36.021 38.0 38.0 38.0 34.0 38.0 85-89 35.92965 38.0 38.0 38.0 34.0 38.0 90-94 35.829950000000004 38.0 38.0 38.0 33.6 38.0 95-99 35.534499999999994 38.0 38.0 38.0 33.0 38.0 100-104 35.3761 38.0 37.2 38.0 31.8 38.0 105-109 35.2557 38.0 37.2 38.0 30.8 38.0 110-114 35.137800000000006 38.0 36.8 38.0 31.0 38.0 115-119 34.9542 38.0 36.2 38.0 29.4 38.0 120-124 34.7327 38.0 36.0 38.0 28.2 38.0 125-129 34.33715 38.0 35.2 38.0 26.0 38.0 130-134 34.14095 38.0 35.0 38.0 24.4 38.0 135-139 33.827999999999996 38.0 35.0 38.0 22.2 38.0 140-144 33.47985 38.0 34.4 38.0 20.2 38.0 145-149 32.57795 38.0 34.0 38.0 13.6 38.0 150-151 28.441625000000002 35.0 24.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 0.0 5 0.0 6 2.0 7 3.0 8 3.0 9 0.0 10 0.0 11 1.0 12 1.0 13 7.0 14 5.0 15 6.0 16 5.0 17 6.0 18 35.0 19 49.0 20 5.0 21 9.0 22 9.0 23 6.0 24 8.0 25 4.0 26 14.0 27 20.0 28 25.0 29 29.0 30 39.0 31 46.0 32 66.0 33 80.0 34 147.0 35 233.0 36 721.0 37 2415.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 48.286524091728936 10.821953104869879 9.430559134243751 31.460963669157433 2 26.575 12.525 30.349999999999998 30.55 3 23.605901475368842 15.57889472368092 25.431357839459867 35.38384596149037 4 26.525 21.349999999999998 25.7 26.424999999999997 5 27.500000000000004 24.0 28.000000000000004 20.5 6 24.50612653163291 29.957489372343087 25.456364091022753 20.080020005001252 7 15.8 25.15 42.699999999999996 16.35 8 20.525 24.3 33.625 21.55 9 20.825 21.5 35.425000000000004 22.25 10-14 23.825 26.52 26.584999999999997 23.07 15-19 21.925 25.535000000000004 28.665000000000003 23.875 20-24 22.615 25.205 28.215 23.965 25-29 21.77 25.230000000000004 29.29 23.71 30-34 21.345 26.779999999999998 28.345 23.53 35-39 22.97 24.87 28.16 24.0 40-44 23.745 25.135 27.33 23.79 45-49 24.03 25.415 28.465 22.09 50-54 24.33 24.395 27.095000000000002 24.18 55-59 22.49 24.775 28.965000000000003 23.77 60-64 21.245 25.89 28.265 24.6 65-69 20.64 28.915000000000003 27.025 23.419999999999998 70-74 22.245 27.295 26.174999999999997 24.285 75-79 22.025 25.845000000000002 27.834999999999997 24.295 80-84 22.81 26.3 27.185 23.705000000000002 85-89 23.669999999999998 25.405 27.33 23.595 90-94 23.34 25.0 28.044999999999998 23.615 95-99 23.09 24.9 28.310000000000002 23.7 100-104 22.985 26.57 26.529999999999998 23.915 105-109 22.675 26.400000000000002 26.919999999999998 24.005000000000003 110-114 21.665 26.229999999999997 26.755000000000003 25.35 115-119 21.745 27.115000000000002 26.545 24.595 120-124 22.564999999999998 26.375 25.7 25.36 125-129 23.53 27.68 25.045 23.745 130-134 23.875 26.155 25.31 24.66 135-139 22.75 27.845 26.619999999999997 22.785 140-144 22.884999999999998 27.715 25.645 23.755000000000003 145-149 22.495 27.575 24.64 25.290000000000003 150-151 22.775000000000002 26.05 25.687500000000004 25.4875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 2.0 3 2.0 4 2.0 5 3.0 6 2.5 7 1.5 8 0.5 9 1.5 10 2.0 11 1.0 12 1.0 13 0.5 14 1.0 15 1.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.5 23 1.5 24 1.5 25 1.5 26 1.0 27 1.5 28 5.5 29 27.0 30 39.0 31 41.5 32 46.5 33 51.0 34 71.0 35 83.0 36 104.5 37 149.5 38 153.0 39 119.0 40 116.5 41 113.0 42 98.0 43 95.0 44 111.0 45 132.5 46 152.5 47 183.0 48 195.0 49 180.0 50 173.0 51 166.0 52 165.5 53 179.5 54 166.0 55 144.5 56 128.0 57 98.0 58 79.0 59 79.5 60 59.5 61 38.0 62 37.5 63 41.0 64 34.5 65 18.0 66 6.0 67 7.5 68 9.5 69 5.5 70 8.0 71 9.5 72 6.5 73 4.0 74 2.5 75 5.5 76 7.5 77 9.0 78 7.5 79 3.0 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.9749999999999996 2 0.0 3 0.025 4 0.0 5 0.0 6 0.025 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.65 #Duplication Level Percentage of deduplicated Percentage of total 1 80.00739098300073 54.125 2 10.606060606060606 14.35 3 4.249815225424982 8.625 4 2.0694752402069474 5.6000000000000005 5 1.0347376201034737 3.5000000000000004 6 0.7760532150776054 3.15 7 0.40650406504065045 1.925 8 0.14781966001478197 0.8 9 0.11086474501108648 0.675 >10 0.5543237250554324 5.35 >50 0.03695491500369549 1.9 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC 76 1.9 TruSeq Adapter, Index 1 (97% over 37bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 37 0.9249999999999999 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 19 0.475 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 18 0.44999999999999996 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 16 0.4 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 14 0.35000000000000003 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 14 0.35000000000000003 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 12 0.3 No Hit CCCTTATGAAGATGTCAATTTAAACGCTATTAAAACCTTGAAAAGTATAT 11 0.27499999999999997 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 11 0.27499999999999997 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 11 0.27499999999999997 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 11 0.27499999999999997 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 10 0.25 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 10 0.25 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 10 0.25 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 10 0.25 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 9 0.22499999999999998 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 9 0.22499999999999998 No Hit CAGGGATATTATTTTATTGCATTGTATTTCATCTTACCCAACCCCTTATG 9 0.22499999999999998 No Hit GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA 8 0.2 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 8 0.2 No Hit GCGCTATCTGCCGGGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGTG 8 0.2 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 8 0.2 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 7 0.17500000000000002 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 7 0.17500000000000002 No Hit GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT 7 0.17500000000000002 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 7 0.17500000000000002 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 7 0.17500000000000002 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 7 0.17500000000000002 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 7 0.17500000000000002 No Hit GGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC 7 0.17500000000000002 No Hit GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA 7 0.17500000000000002 No Hit CGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTG 7 0.17500000000000002 No Hit GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG 7 0.17500000000000002 No Hit GTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTG 6 0.15 No Hit CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA 6 0.15 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 6 0.15 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 6 0.15 No Hit GGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTG 6 0.15 No Hit GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA 6 0.15 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 6 0.15 No Hit TGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATG 6 0.15 No Hit GTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACAC 6 0.15 No Hit GGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGGCAATTTCAAGCACT 6 0.15 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 6 0.15 No Hit CTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTT 6 0.15 No Hit CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT 6 0.15 No Hit GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG 6 0.15 No Hit CCCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGGCT 6 0.15 No Hit GCGACAGTTCATCATTAAAGATGAGGAGATCAGCTTCAAGCTCTTCCACG 6 0.15 No Hit GCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTA 6 0.15 No Hit GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC 6 0.15 No Hit GCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGT 6 0.15 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 6 0.15 No Hit GCCCCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGG 6 0.15 No Hit GGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATAATTTTAGTATC 5 0.125 No Hit GCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 5 0.125 No Hit CCGCATACGGCCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGGC 5 0.125 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 5 0.125 No Hit GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT 5 0.125 No Hit GCCGTTCCGCATACGGCCAGGACGCATCGCCGGCCCCCATCCGCTTCCCT 5 0.125 No Hit GTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTG 5 0.125 No Hit GTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCG 5 0.125 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 5 0.125 No Hit ATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGA 5 0.125 No Hit ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT 5 0.125 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 5 0.125 No Hit CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA 5 0.125 No Hit CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA 5 0.125 No Hit GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG 5 0.125 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 5 0.125 No Hit GGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTC 5 0.125 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 5 0.125 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG 5 0.125 TruSeq Adapter, Index 1 (97% over 37bp) GGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAG 5 0.125 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 5 0.125 No Hit TGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATC 5 0.125 No Hit GTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTA 5 0.125 No Hit GTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCAC 5 0.125 No Hit CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC 5 0.125 No Hit CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT 5 0.125 No Hit GTCCATAACACGATCTAGTGAAACCTTCAAGCATGAATTGTAGCTGACGG 5 0.125 No Hit ACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.125 0.0 0.0 0.0 0.0 2 0.125 0.0 0.0 0.0 0.0 3 0.125 0.0 0.0 0.0 0.0 4 0.125 0.0 0.0 0.0 0.0 5 0.125 0.0 0.0 0.0 0.0 6 0.125 0.0 0.0 0.0 0.0 7 0.125 0.0 0.0 0.0 0.0 8 0.125 0.0 0.0 0.0 0.0 9 0.125 0.0 0.0 0.0 0.0 10-11 0.125 0.0 0.0 0.0 0.0 12-13 0.125 0.0 0.0 0.0 0.0 14-15 0.125 0.0 0.0 0.0 0.0 16-17 0.125 0.0 0.0 0.0 0.0 18-19 0.125 0.0 0.0 0.0 0.0 20-21 0.125 0.0 0.0 0.0 0.0 22-23 0.125 0.0 0.0 0.0 0.0 24-25 0.125 0.0 0.0 0.0 0.0 26-27 0.125 0.0 0.0 0.0 0.0 28-29 0.125 0.0 0.0 0.0 0.0 30-31 0.125 0.0 0.0 0.0 0.0 32-33 0.125 0.0 0.0 0.0 0.0 34-35 0.125 0.0 0.0 0.0 0.0 36-37 0.125 0.0 0.0 0.0 0.0 38-39 0.125 0.0 0.0 0.0 0.0 40-41 0.125 0.0 0.0 0.0 0.0 42-43 0.125 0.0 0.0 0.0 0.0 44-45 0.125 0.0 0.0 0.0 0.0 46-47 0.125 0.0 0.0 0.0 0.0 48-49 0.125 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.16249999999999998 0.0 0.0 0.0 0.0 68-69 0.2 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.30000000000000004 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.44999999999999996 0.0 0.0 0.0 0.0 82-83 0.525 0.0 0.0 0.0 0.0 84-85 0.6625 0.0 0.0 0.0 0.0 86-87 0.875 0.0 0.0 0.0 0.0 88-89 1.0125 0.0 0.0 0.0 0.0 90-91 1.15 0.0 0.0 0.0 0.0 92-93 1.5 0.0 0.0 0.0 0.0 94-95 1.775 0.0 0.0 0.0 0.0 96-97 2.0125 0.0 0.0 0.0 0.0 98-99 2.3375 0.0 0.0 0.0 0.0 100-101 2.6125 0.0 0.0 0.0 0.0 102-103 2.9625 0.0 0.0 0.0 0.0 104-105 3.25 0.0 0.0 0.0 0.0 106-107 3.625 0.0 0.0 0.0 0.0 108-109 4.2375 0.0 0.0 0.0 0.0 110-111 4.625 0.0 0.0 0.0 0.0 112-113 5.1 0.0 0.0 0.0 0.0 114-115 5.4875 0.0 0.0 0.0 0.0 116-117 6.137499999999999 0.0 0.0 0.0 0.0 118-119 6.75 0.0 0.0 0.0 0.0 120-121 7.4 0.0 0.0 0.0 0.0 122-123 8.2 0.0 0.0 0.0 0.0 124-125 9.037500000000001 0.0 0.0 0.0 0.0 126-127 9.8125 0.0 0.0 0.0 0.0 128-129 10.6375 0.0 0.0 0.0 0.0 130-131 11.45 0.0 0.0 0.0 0.0 132-133 12.0875 0.0 0.0 0.0 0.0 134-135 12.837499999999999 0.0 0.0 0.0 0.0 136-137 13.6125 0.0 0.0 0.0 0.0 138-139 14.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGC 10 0.006832588 144.9875 7 TGCGCAG 10 0.006832588 144.9875 9 GGACCTG 10 0.006832588 144.9875 145 TAGCTAG 10 0.006832588 144.9875 9 ATAGCTA 10 0.006832588 144.9875 8 CTGCGCA 10 0.006832588 144.9875 8 TCCGCAT 10 0.006832588 144.9875 3 CGCATAG 10 0.006832588 144.9875 5 CTCCGCA 10 0.006832588 144.9875 2 >>END_MODULE SRR5578469 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578469_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.94625 33.0 33.0 34.0 32.0 34.0 2 32.987 34.0 33.0 34.0 32.0 34.0 3 32.99325 34.0 33.0 34.0 32.0 34.0 4 32.84175 34.0 33.0 34.0 32.0 34.0 5 32.90925 34.0 33.0 34.0 32.0 34.0 6 37.0955 38.0 38.0 38.0 37.0 38.0 7 37.00625 38.0 38.0 38.0 37.0 38.0 8 37.044 38.0 38.0 38.0 37.0 38.0 9 37.08975 38.0 38.0 38.0 37.0 38.0 10-14 37.02645 38.0 38.0 38.0 37.0 38.0 15-19 36.9206 38.0 38.0 38.0 37.0 38.0 20-24 36.9423 38.0 38.0 38.0 37.0 38.0 25-29 37.00335 38.0 38.0 38.0 37.0 38.0 30-34 36.9724 38.0 38.0 38.0 37.0 38.0 35-39 36.95685 38.0 38.0 38.0 37.0 38.0 40-44 36.9948 38.0 38.0 38.0 37.0 38.0 45-49 36.9072 38.0 38.0 38.0 37.0 38.0 50-54 36.86095 38.0 38.0 38.0 36.8 38.0 55-59 36.86899999999999 38.0 38.0 38.0 36.8 38.0 60-64 36.92545 38.0 38.0 38.0 37.0 38.0 65-69 36.59705 38.0 38.0 38.0 36.2 38.0 70-74 36.03455 38.0 38.0 38.0 35.0 38.0 75-79 36.01155 38.0 38.0 38.0 35.0 38.0 80-84 35.8885 38.0 38.0 38.0 34.8 38.0 85-89 35.83 38.0 38.0 38.0 34.2 38.0 90-94 35.72835 38.0 38.0 38.0 34.0 38.0 95-99 35.65205 38.0 38.0 38.0 33.8 38.0 100-104 35.5281 38.0 38.0 38.0 33.4 38.0 105-109 35.40435 38.0 38.0 38.0 33.0 38.0 110-114 35.23285 38.0 38.0 38.0 31.6 38.0 115-119 35.02545 38.0 37.4 38.0 30.2 38.0 120-124 34.895950000000006 38.0 36.6 38.0 29.8 38.0 125-129 34.643899999999995 38.0 36.0 38.0 28.0 38.0 130-134 34.273399999999995 38.0 35.4 38.0 25.0 38.0 135-139 33.8527 38.0 34.4 38.0 23.0 38.0 140-144 33.248999999999995 38.0 33.0 38.0 17.8 38.0 145-149 32.1389 38.0 33.0 38.0 8.2 38.0 150-151 27.539625 34.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 6.0 4 7.0 5 4.0 6 1.0 7 3.0 8 2.0 9 2.0 10 3.0 11 4.0 12 5.0 13 6.0 14 6.0 15 10.0 16 18.0 17 66.0 18 5.0 19 5.0 20 2.0 21 4.0 22 5.0 23 11.0 24 9.0 25 17.0 26 14.0 27 17.0 28 20.0 29 28.0 30 32.0 31 41.0 32 51.0 33 83.0 34 125.0 35 202.0 36 587.0 37 2588.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.35 21.775 12.55 27.325 2 29.889944972486244 25.86293146573287 24.212106053026513 20.035017508754375 3 23.123123123123122 25.5005005005005 27.47747747747748 23.8988988988989 4 24.455569461827285 31.464330413016274 22.428035043804755 21.65206508135169 5 27.77082812109082 34.15061295971979 19.764823617713283 18.313735301476108 6 24.90622655663916 35.45886471617904 20.905226306576644 18.72968242060515 7 19.979994998749685 23.10577644411103 34.98374593648413 21.930482620655166 8 23.125 28.1 23.724999999999998 25.05 9 26.281570392598148 24.10602650662666 27.431857964491122 22.18054513628407 10-14 25.39912917271408 27.631249687202843 22.50638106200891 24.46324007807417 15-19 25.792875394558845 26.529385239741472 24.530287088531487 23.147452277168195 20-24 25.702549716976407 27.586034163201923 23.854130140760407 22.857285979061263 25-29 26.405451448040886 27.51778735344223 23.46427497745265 22.612486221064234 30-34 26.67669172932331 26.671679197994987 24.461152882205514 22.19047619047619 35-39 26.355617921218805 25.754234739901776 24.03026962012629 23.859877718753133 40-44 27.061533069644018 26.095228558554044 24.04245731737846 22.80078105442347 45-49 25.463009310241265 25.523075382921213 25.082590849934927 23.93132445690259 50-54 24.530475284218962 25.697400711173437 26.603896429108026 23.168227575499575 55-59 23.681706645300217 27.302318593820424 26.666332815864592 22.349641945014774 60-64 23.08617234468938 28.22144288577154 26.152304609218437 22.54008016032064 65-69 23.473990177408037 27.95930640473088 25.468577728776186 23.098125689084895 70-74 23.326317899378633 28.307276007215876 24.80958107837242 23.556825015033073 75-79 22.24897181261912 29.150366135018558 24.962383388504364 23.63827866385796 80-84 23.717337880535634 28.130798936757106 24.66522894829229 23.486634234414964 85-89 23.1508838699985 28.05849066052381 25.744904602133307 23.045720867344382 90-94 24.684684684684687 28.923923923923923 24.48948948948949 21.9019019019019 95-99 23.80260521042084 29.428857715430862 25.035070140280563 21.733466933867735 100-104 23.77755511022044 30.34068136272545 24.113226452905813 21.7685370741483 105-109 24.230884858202227 30.293616594849183 24.11564284998497 21.359855696963624 110-114 24.187562688064194 30.110330992978934 22.662988966900702 23.039117352056167 115-119 24.55014786226254 30.92576813192321 22.64046914941607 21.883614856398175 120-124 25.154112163584426 29.689770961760136 23.264671979151004 21.891444895504435 125-129 25.324447562258857 30.265069900285614 22.84912562008318 21.56135691737235 130-134 25.43323650205349 28.29309826705399 24.3413803465892 21.932284884303314 135-139 24.73973973973974 29.234234234234236 24.34934934934935 21.676676676676678 140-144 26.757040668300736 27.657445850632783 24.481016457405833 21.10449702366065 145-149 26.843185344611843 27.744131337904797 24.155363131287853 21.257320186195507 150-151 26.86921730432608 27.60690172543136 24.543635908977244 20.980245061265315 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.5 5 0.5 6 0.5 7 1.0 8 0.5 9 0.0 10 0.5 11 1.0 12 0.5 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 0.5 19 1.0 20 1.5 21 1.0 22 1.5 23 2.5 24 1.5 25 0.5 26 2.0 27 4.0 28 8.0 29 9.0 30 12.5 31 25.5 32 34.0 33 30.0 34 38.5 35 62.0 36 91.0 37 127.5 38 151.5 39 161.0 40 207.0 41 170.0 42 91.5 43 89.5 44 96.0 45 106.5 46 109.5 47 140.0 48 190.0 49 188.0 50 159.0 51 155.5 52 158.5 53 178.0 54 204.5 55 185.5 56 157.5 57 127.5 58 98.0 59 83.5 60 57.5 61 41.5 62 28.0 63 23.5 64 25.0 65 20.5 66 15.0 67 14.5 68 18.0 69 17.0 70 14.5 71 10.5 72 4.5 73 4.0 74 7.0 75 5.5 76 8.0 77 8.0 78 3.5 79 2.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.1 4 0.125 5 0.075 6 0.025 7 0.025 8 0.0 9 0.025 10-14 0.095 15-19 0.20500000000000002 20-24 0.185 25-29 0.21 30-34 0.25 35-39 0.22999999999999998 40-44 0.135 45-49 0.11 50-54 0.165 55-59 0.155 60-64 0.2 65-69 0.22999999999999998 70-74 0.22 75-79 0.31 80-84 0.305 85-89 0.155 90-94 0.1 95-99 0.2 100-104 0.2 105-109 0.21 110-114 0.3 115-119 0.245 120-124 0.23500000000000001 125-129 0.215 130-134 0.16999999999999998 135-139 0.1 140-144 0.045 145-149 0.105 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 79.45046439628483 51.324999999999996 2 11.493808049535604 14.85 3 4.179566563467492 8.1 4 1.8962848297213624 4.9 5 0.541795665634675 1.7500000000000002 6 0.7352941176470588 2.85 7 0.2708978328173375 1.225 8 0.2708978328173375 1.4000000000000001 9 0.2708978328173375 1.575 >10 0.8513931888544891 10.05 >50 0.03869969040247678 1.975 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 79 1.975 Illumina Single End PCR Primer 1 (100% over 50bp) CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 34 0.8500000000000001 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 27 0.675 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 26 0.65 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 25 0.625 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 24 0.6 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 23 0.575 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 23 0.575 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 22 0.5499999999999999 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 22 0.5499999999999999 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 21 0.525 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 20 0.5 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 15 0.375 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 14 0.35000000000000003 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 13 0.325 No Hit TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA 13 0.325 No Hit ATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTA 13 0.325 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 12 0.3 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 12 0.3 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 11 0.27499999999999997 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 11 0.27499999999999997 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 11 0.27499999999999997 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 10 0.25 No Hit GTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTT 9 0.22499999999999998 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 9 0.22499999999999998 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 9 0.22499999999999998 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 9 0.22499999999999998 No Hit GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA 9 0.22499999999999998 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 9 0.22499999999999998 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 9 0.22499999999999998 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 8 0.2 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 8 0.2 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 8 0.2 No Hit GGGCGGAAGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGT 8 0.2 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 8 0.2 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 8 0.2 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 8 0.2 No Hit AAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT 7 0.17500000000000002 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 7 0.17500000000000002 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 7 0.17500000000000002 No Hit CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC 7 0.17500000000000002 No Hit CACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGG 7 0.17500000000000002 No Hit AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC 7 0.17500000000000002 No Hit GCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCC 7 0.17500000000000002 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 6 0.15 No Hit AATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCA 6 0.15 No Hit ATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG 6 0.15 No Hit CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTG 6 0.15 No Hit CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG 6 0.15 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 6 0.15 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 6 0.15 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 6 0.15 No Hit GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA 6 0.15 No Hit ATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT 6 0.15 No Hit CGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGG 6 0.15 No Hit ATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCAT 6 0.15 No Hit CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC 6 0.15 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 6 0.15 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 6 0.15 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 6 0.15 Illumina Single End PCR Primer 1 (100% over 50bp) GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 6 0.15 No Hit GATAAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATG 6 0.15 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 6 0.15 No Hit GGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC 5 0.125 No Hit CCGGGCGGAAGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCT 5 0.125 No Hit GTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC 5 0.125 No Hit CATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCT 5 0.125 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 5 0.125 No Hit ATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGAC 5 0.125 No Hit GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT 5 0.125 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 5 0.125 No Hit CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC 5 0.125 No Hit GCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTAT 5 0.125 No Hit GAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAA 5 0.125 No Hit CCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATA 5 0.125 No Hit AACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAAC 5 0.125 No Hit CTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.15 0.0 0.0 0.0 0.0 2 0.15 0.0 0.0 0.0 0.0 3 0.15 0.0 0.0 0.0 0.0 4 0.15 0.0 0.0 0.0 0.0 5 0.15 0.0 0.0 0.0 0.0 6 0.15 0.0 0.0 0.0 0.0 7 0.15 0.0 0.0 0.0 0.0 8 0.15 0.0 0.0 0.0 0.0 9 0.15 0.0 0.0 0.0 0.0 10-11 0.15 0.0 0.0 0.0 0.0 12-13 0.15 0.0 0.0 0.0 0.0 14-15 0.15 0.0 0.0 0.0 0.0 16-17 0.15 0.0 0.0 0.0 0.0 18-19 0.15 0.0 0.0 0.0 0.0 20-21 0.15 0.0 0.0 0.0 0.0 22-23 0.15 0.0 0.0 0.0 0.0 24-25 0.15 0.0 0.0 0.0 0.0 26-27 0.15 0.0 0.0 0.0 0.0 28-29 0.15 0.0 0.0 0.0 0.0 30-31 0.15 0.0 0.0 0.0 0.0 32-33 0.15 0.0 0.0 0.0 0.0 34-35 0.15 0.0 0.0 0.0 0.0 36-37 0.15 0.0 0.0 0.0 0.0 38-39 0.15 0.0 0.0 0.0 0.0 40-41 0.15 0.0 0.0 0.0 0.0 42-43 0.15 0.0 0.0 0.0 0.0 44-45 0.15 0.0 0.0 0.0 0.0 46-47 0.15 0.0 0.0 0.0 0.0 48-49 0.15 0.0 0.0 0.0 0.0 50-51 0.15 0.0 0.0 0.0 0.0 52-53 0.15 0.0 0.0 0.0 0.0 54-55 0.15 0.0 0.0 0.0 0.0 56-57 0.15 0.0 0.0 0.0 0.0 58-59 0.15 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.16249999999999998 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.2 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.30000000000000004 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.55 0.0 0.0 0.0 0.0 84-85 0.6875 0.0 0.0 0.0 0.0 86-87 0.8875 0.0 0.0 0.0 0.0 88-89 1.0125 0.0 0.0 0.0 0.0 90-91 1.175 0.0 0.0 0.0 0.0 92-93 1.5499999999999998 0.0 0.0 0.0 0.0 94-95 1.8 0.0 0.0 0.0 0.0 96-97 2.0375 0.0 0.0 0.0 0.0 98-99 2.3625 0.0 0.0 0.0 0.0 100-101 2.625 0.0 0.0 0.0 0.0 102-103 3.0250000000000004 0.0 0.0 0.0 0.0 104-105 3.3625 0.0 0.0 0.0 0.0 106-107 3.7375 0.0 0.0 0.0 0.0 108-109 4.324999999999999 0.0 0.0 0.0 0.0 110-111 4.725 0.0 0.0 0.0 0.0 112-113 5.1875 0.0 0.0 0.0 0.0 114-115 5.574999999999999 0.0 0.0 0.0 0.0 116-117 6.175000000000001 0.0 0.0 0.0 0.0 118-119 6.737500000000001 0.0 0.0 0.0 0.0 120-121 7.3625 0.0 0.0 0.0 0.0 122-123 8.162500000000001 0.0 0.0 0.0 0.0 124-125 9.0375 0.0 0.0 0.0 0.0 126-127 9.912500000000001 0.0 0.0 0.0 0.0 128-129 10.75 0.0 0.0 0.0 0.0 130-131 11.587499999999999 0.0 0.0 0.0 0.0 132-133 12.274999999999999 0.0 0.0 0.0 0.0 134-135 13.075 0.0 0.0 0.0 0.0 136-137 13.875 0.0 0.0 0.0 0.0 138-139 14.525 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra Read 605794 spots for SRR5578469.sra Written 605794 spots for SRR5578469.sra SRR ids: ['SRR5578469.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_i7jquppe SRR5578469.sra spots: 12115880 blocks: [[1, 605794], [605795, 1211588], [1211589, 1817382], [1817383, 2423176], [2423177, 3028970], [3028971, 3634764], [3634765, 4240558], [4240559, 4846352], [4846353, 5452146], [5452147, 6057940], [6057941, 6663734], [6663735, 7269528], [7269529, 7875322], [7875323, 8481116], [8481117, 9086910], [9086911, 9692704], [9692705, 10298498], [10298499, 10904292], [10904293, 11510086], [11510087, 12115880]] SRR5578469 file size 4083973 SRR5578469 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578469 SRR5578469_1.fastq SRR5578469_2.fastq Input file: SRR5578469_1.fastq Paired file: SRR5578469_2.fastq trimmed: SRR5578469-trimmed-pair1.fastq, SRR5578469-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 18:59:31 2024 >> started Mon Dec 9 19:00:23 2024 >> done (52.131s) 12115880 read pairs processed; of these: 28060 ( 0.23%) short read pairs filtered out after trimming by size control 258314 ( 2.13%) empty read pairs filtered out after trimming by size control 11829506 (97.64%) read pairs available; of these: 6390785 (54.02%) trimmed read pairs available after processing 5438721 (45.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 10 0.00% 20 19 0.00% 21 24 0.00% 22 20 0.00% 23 44 0.00% 24 42 0.00% 25 25 0.00% 26 26 0.00% 27 27 0.00% 28 20 0.00% 29 51 0.00% 30 40 0.00% 31 58 0.00% 32 34 0.00% 33 38 0.00% 34 23 0.00% 35 88 0.00% 36 54 0.00% 37 50 0.00% 38 50 0.00% 39 42 0.00% 40 96 0.00% 41 75 0.00% 42 83 0.00% 43 112 0.00% 44 141 0.00% 45 201 0.00% 46 208 0.00% 47 243 0.00% 48 265 0.00% 49 270 0.00% 50 310 0.00% 51 301 0.00% 52 399 0.00% 53 413 0.00% 54 482 0.00% 55 370 0.00% 56 465 0.00% 57 477 0.00% 58 589 0.00% 59 549 0.00% 60 687 0.01% 61 781 0.01% 62 829 0.01% 63 941 0.01% 64 1206 0.01% 65 2002 0.02% 66 2252 0.02% 67 2757 0.02% 68 4328 0.04% 69 16917 0.14% 70 20186 0.17% 71 7389 0.06% 72 4635 0.04% 73 4030 0.03% 74 3989 0.03% 75 4156 0.04% 76 4299 0.04% 77 4825 0.04% 78 5181 0.04% 79 5926 0.05% 80 6051 0.05% 81 6831 0.06% 82 7974 0.07% 83 8769 0.07% 84 10535 0.09% 85 12106 0.10% 86 13166 0.11% 87 14636 0.12% 88 16049 0.14% 89 16918 0.14% 90 17472 0.15% 91 17246 0.15% 92 18726 0.16% 93 19722 0.17% 94 20510 0.17% 95 22161 0.19% 96 23589 0.20% 97 24243 0.20% 98 25786 0.22% 99 26397 0.22% 100 28406 0.24% 101 28942 0.24% 102 29613 0.25% 103 31743 0.27% 104 32959 0.28% 105 34257 0.29% 106 36333 0.31% 107 37198 0.31% 108 38990 0.33% 109 38375 0.32% 110 40041 0.34% 111 40902 0.35% 112 44080 0.37% 113 48227 0.41% 114 50125 0.42% 115 53521 0.45% 116 51803 0.44% 117 51454 0.43% 118 49168 0.42% 119 48853 0.41% 120 51879 0.44% 121 51010 0.43% 122 54966 0.46% 123 56532 0.48% 124 57152 0.48% 125 58357 0.49% 126 59665 0.50% 127 59226 0.50% 128 56597 0.48% 129 60928 0.52% 130 60227 0.51% 131 59524 0.50% 132 61470 0.52% 133 65159 0.55% 134 67226 0.57% 135 67943 0.57% 136 67308 0.57% 137 67402 0.57% 138 72815 0.62% 139 75883 0.64% 140 76546 0.65% 141 77878 0.66% 142 91082 0.77% 143 92135 0.78% 144 98767 0.83% 145 110946 0.94% 146 132019 1.12% 147 162451 1.37% 148 230856 1.95% 149 456215 3.86% 150 2382582 20.14% 151 5438721 45.98% 11829506 reads passed initial QC criterion=sequence-density sequence-density=0.55 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=31 prefix-density=0.55 prefix-fanout=1.9 sequence=AGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAATAAATGCGCCCCTCCCAGAAGTCGGGGTTTGTTGCACGTATTAGCTCTAGAATTACTA criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=25.67 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=1.0 sequence=CTTTGATTTGGATGAGATAATTAACGATACAACCGTTCCAAGAAACATTAGAGCTGCTGCTGAAAAGGCTAAAGAAGCTGTTTTAAAAGAGGGGGAGGAGCCAATCGTTAGAAGTGCAACAGCAATCCACATCTTAGATGAGATTAGCAACGACCCAAACATGCCACTTCACACAAGAACACAAATTTGGAGTATTGTTAGTGAATTAGAAAGAGTTAAATAAATTTAAAAATCCCCACTATTTCTTTACAAGAAGGTTTAAAAGTGTAAGTT criterion=sequence-density sequence-density=0.87 sequence-density-rank=1 fanout-score=4.72 fanout-score-rank=9 prefix-density=4.12 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.11 sequence-density-rank=17 fanout-score=39.67 fanout-score-rank=1 prefix-density=4.08 prefix-fanout=1.1 sequence=GGGCTGAAAGCACAGGTGGCATATGCGAGCAAAGCATTCTCATCAGTCGCAATGATTCAGCTCGCTGAGGAAGAGGGACTTTCTTTAGATGTCGTATCCGGAGGAGAGCTATATACGGCTGTTGCAGCAGGCTTTCCGGCAGAACGCATCCACTTTCATGGAAACAATAAGAGCAGGGAAGAACTGCGGATGGCGCTTGAGCACCGCATCGGCTGCATTGTGGTGGATAATTTCTATGAAATCGCGCTTCTTGAAGACCTATGTAAAGAAACGGGTCACTCCATCGATGTTCTTCTTCGGATCACGCCCGGAGTAGAAGCGCATACGCATGACTACATTACAACGGGCCAGGAAGATTCAAAGTTTGGTTTCGATCTTCATAACGGACAAACTGAACGGGCCATTGAACAAGTATTACAATCGGAACACATTCAGCTGCTGGGTGTCCATTGCCATATCGGCTCGCAAATCTTTGATACGGCCGGTTTTGTGTTAGCAGCGGAAAAAATCT SRR5578469 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 19:02:36 Started mapping on | Dec 09 19:02:37 Finished on | Dec 09 19:39:20 Mapping speed, Million of reads per hour | 19.33 Number of input reads | 11829506 Average input read length | 285 UNIQUE READS: Uniquely mapped reads number | 1369286 Uniquely mapped reads % | 11.58% Average mapped length | 286.73 Number of splices: Total | 887602 Number of splices: Annotated (sjdb) | 832116 Number of splices: GT/AG | 876886 Number of splices: GC/AG | 9238 Number of splices: AT/AC | 482 Number of splices: Non-canonical | 996 Mismatch rate per base, % | 0.13% Deletion rate per base | 0.01% Deletion average length | 1.49 Insertion rate per base | 0.00% Insertion average length | 1.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 326547 % of reads mapped to multiple loci | 2.76% Number of reads mapped to too many loci | 257060 % of reads mapped to too many loci | 2.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 74.35% % of reads unmapped: other | 9.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10135573 10135573 10135573 N_multimapping 326547 326547 326547 N_noFeature 369157 1338416 380372 N_ambiguous 35482 1087 16661 UnstrandedReadsAssigned:964647 PositiveStrandReadsAssigned:29783 NegativeStrandReadsAssigned:972253 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=139 echo kmer=135 SRR5578469 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578469-trimmed-pair1.fastq SRR5578469-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 11,829,506 reads, 1,058,681 reads pseudoaligned [quant] estimated average fragment length: 216.256 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,035 rounds 52973 SRR5578469.ke.tsv 35125 SRR5578469.se.tsv 88098 total ==> SRR5578469.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 720.941 6.63861 10.4155 PNS24247 1044 828.744 0 0 PNS24249 1928 1712.74 10.3614 6.84268 PNS24246 1044 828.744 0 0 PNS24248 1044 828.744 0 0 PNS24244 1471 1255.74 0 0 PNS24243 293 112.935 0 0 KQK14069 1603 1387.74 491 400.196 KQK14071 474 268.091 0 0 ==> SRR5578469.se.tsv <== BRADI_1g14170v3 492 BRADI_1g53295v3 0 BRADI_1g59795v3 12 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 273 BRADI_1g74790v3 1 BRADI_1g09890v3 0 BRADI_1g77505v3 12 BRADI_1g48960v3 0 SRR5578469 completed mapping pipeline successfully