Starting /dee2/code/volunteer_pipeline.sh SRR5578471 current disk space = 1522413940736 free memory = 1580361212 SRR5578471 SRAfilesize 080d1cb0bc0359530028b3fc27e44538 SRR5578471.sra SRR5578471.sra file validated SRR5578471 is paired end SRR5578471 is conventional basespace SRR5578471 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578471_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.33275 34.0 33.0 34.0 33.0 34.0 2 33.478 34.0 34.0 34.0 33.0 34.0 3 33.47975 34.0 34.0 34.0 33.0 34.0 4 33.487 34.0 34.0 34.0 33.0 34.0 5 33.4985 34.0 34.0 34.0 33.0 34.0 6 37.22475 38.0 38.0 38.0 36.0 38.0 7 37.434 38.0 38.0 38.0 37.0 38.0 8 37.5095 38.0 38.0 38.0 37.0 38.0 9 37.55475 38.0 38.0 38.0 38.0 38.0 10-14 37.5073 38.0 38.0 38.0 38.0 38.0 15-19 37.50725 38.0 38.0 38.0 37.8 38.0 20-24 37.490899999999996 38.0 38.0 38.0 38.0 38.0 25-29 37.4131 38.0 38.0 38.0 37.8 38.0 30-34 37.341899999999995 38.0 38.0 38.0 37.2 38.0 35-39 37.3116 38.0 38.0 38.0 37.0 38.0 40-44 37.1463 38.0 38.0 38.0 37.0 38.0 45-49 37.19345 38.0 38.0 38.0 37.0 38.0 50-54 37.19885000000001 38.0 38.0 38.0 37.0 38.0 55-59 37.1197 38.0 38.0 38.0 37.0 38.0 60-64 37.12305 38.0 38.0 38.0 37.0 38.0 65-69 37.08335 38.0 38.0 38.0 36.8 38.0 70-74 36.85435 38.0 38.0 38.0 36.4 38.0 75-79 36.26855 38.0 38.0 38.0 35.8 38.0 80-84 36.177800000000005 38.0 38.0 38.0 35.2 38.0 85-89 36.0848 38.0 38.0 38.0 34.8 38.0 90-94 36.001349999999995 38.0 38.0 38.0 34.2 38.0 95-99 35.98535 38.0 38.0 38.0 34.4 38.0 100-104 35.8633 38.0 38.0 38.0 34.0 38.0 105-109 35.8585 38.0 38.0 38.0 34.0 38.0 110-114 35.79665 38.0 38.0 38.0 34.0 38.0 115-119 35.59805 38.0 38.0 38.0 33.2 38.0 120-124 35.4677 38.0 38.0 38.0 32.8 38.0 125-129 35.3408 38.0 38.0 38.0 32.4 38.0 130-134 35.057249999999996 38.0 37.2 38.0 31.0 38.0 135-139 34.98785 38.0 37.6 38.0 30.6 38.0 140-144 34.7983 38.0 36.6 38.0 29.4 38.0 145-149 34.286950000000004 38.0 36.0 38.0 27.2 38.0 150-151 30.791375000000002 35.5 30.5 38.0 11.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 4.0 8 4.0 9 4.0 10 4.0 11 1.0 12 4.0 13 0.0 14 1.0 15 3.0 16 5.0 17 11.0 18 27.0 19 48.0 20 6.0 21 8.0 22 13.0 23 9.0 24 11.0 25 6.0 26 8.0 27 17.0 28 16.0 29 19.0 30 33.0 31 37.0 32 40.0 33 66.0 34 82.0 35 154.0 36 338.0 37 3020.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.6180904522613 11.4321608040201 8.944723618090453 26.00502512562814 2 25.3 14.249999999999998 31.275 29.175 3 22.85 18.65 26.8 31.7 4 25.124999999999996 23.825 25.650000000000002 25.4 5 25.95 26.674999999999997 27.025 20.349999999999998 6 21.425 32.25 27.700000000000003 18.625 7 15.775 25.074999999999996 41.775 17.375 8 18.5 27.375 32.300000000000004 21.825 9 20.525 21.575 36.475 21.425 10-14 22.715 28.560000000000002 26.52 22.205 15-19 22.235 26.71 27.325 23.73 20-24 22.515 25.965 28.115000000000002 23.405 25-29 21.584999999999997 27.150000000000002 28.725 22.54 30-34 21.025 27.615000000000002 27.515 23.845 35-39 21.82 27.084999999999997 27.35 23.745 40-44 23.155 26.395000000000003 26.93 23.52 45-49 23.595 27.339999999999996 27.865000000000002 21.2 50-54 24.285 26.700000000000003 25.900000000000002 23.115 55-59 22.56 26.26 27.950000000000003 23.23 60-64 20.71 27.755000000000003 27.485 24.05 65-69 21.025 29.98 25.729999999999997 23.265 70-74 22.255 28.945 25.31 23.49 75-79 22.015 27.405 26.334999999999997 24.245 80-84 23.150000000000002 28.17 25.81 22.869999999999997 85-89 23.53 27.034999999999997 26.32 23.115 90-94 22.745 26.490000000000002 27.11 23.655 95-99 21.535 26.58 28.51 23.375 100-104 22.935 26.85 26.450000000000003 23.765 105-109 22.29 28.49 26.275 22.945 110-114 21.935 27.51 25.705 24.85 115-119 21.435000000000002 28.499999999999996 25.974999999999998 24.09 120-124 23.225 27.450000000000003 24.715 24.610000000000003 125-129 22.36 28.1 24.69 24.85 130-134 23.57 27.08 25.09 24.26 135-139 21.785 27.72 27.21 23.285 140-144 22.91 27.860000000000003 25.374999999999996 23.855 145-149 22.2 28.810000000000002 23.935000000000002 25.055 150-151 22.925 26.787499999999998 24.175 26.1125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 1.0 5 1.5 6 2.0 7 1.5 8 1.0 9 0.5 10 1.0 11 1.0 12 0.5 13 0.5 14 0.0 15 0.5 16 0.5 17 1.0 18 1.0 19 1.0 20 2.0 21 3.5 22 2.5 23 0.5 24 2.0 25 4.5 26 4.0 27 3.0 28 4.0 29 17.0 30 33.5 31 54.5 32 67.0 33 74.5 34 95.5 35 104.0 36 135.5 37 176.5 38 174.0 39 147.0 40 156.5 41 147.5 42 113.5 43 107.5 44 113.5 45 139.0 46 146.0 47 160.0 48 171.5 49 166.0 50 149.0 51 132.5 52 136.0 53 145.0 54 131.0 55 97.5 56 84.5 57 69.0 58 57.5 59 64.0 60 52.5 61 33.5 62 31.0 63 35.5 64 33.5 65 25.5 66 20.0 67 18.0 68 25.0 69 22.0 70 16.5 71 16.0 72 15.5 73 13.5 74 6.5 75 5.5 76 5.5 77 4.5 78 5.0 79 3.5 80 1.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.55000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 87.35554044867438 64.25 2 6.9680489462950375 10.25 3 2.175390890550646 4.8 4 1.1896668932698844 3.5000000000000004 5 0.7817811012916385 2.875 6 0.2379333786539769 1.05 7 0.2379333786539769 1.225 8 0.20394289598912305 1.2 9 0.13596193065941536 0.8999999999999999 >10 0.6798096532970768 7.675 >50 0.03399048266485384 2.275 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCATATCTCGTATGC 91 2.275 TruSeq Adapter, Index 4 (97% over 37bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 50 1.25 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 25 0.625 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 20 0.5 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 18 0.44999999999999996 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 15 0.375 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 14 0.35000000000000003 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 14 0.35000000000000003 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 14 0.35000000000000003 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 13 0.325 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 13 0.325 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 13 0.325 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 13 0.325 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 12 0.3 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 12 0.3 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 11 0.27499999999999997 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 10 0.25 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 10 0.25 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 10 0.25 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 10 0.25 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 10 0.25 No Hit GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT 9 0.22499999999999998 No Hit GGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAAT 9 0.22499999999999998 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 9 0.22499999999999998 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 9 0.22499999999999998 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 8 0.2 No Hit GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT 8 0.2 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 8 0.2 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 8 0.2 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 8 0.2 No Hit CAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAA 8 0.2 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 7 0.17500000000000002 No Hit GTCCAAAGTTAAGCTCTGCACCTGTTACACTATAGTCATCGTCCATAACA 7 0.17500000000000002 No Hit GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA 7 0.17500000000000002 No Hit CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT 7 0.17500000000000002 No Hit GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA 7 0.17500000000000002 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 7 0.17500000000000002 No Hit GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA 7 0.17500000000000002 No Hit CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA 6 0.15 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 6 0.15 No Hit GCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTG 6 0.15 No Hit AACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTATATTTCGT 6 0.15 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 6 0.15 No Hit GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT 6 0.15 No Hit GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT 6 0.15 No Hit AAAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATATTC 5 0.125 No Hit CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC 5 0.125 No Hit CAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCA 5 0.125 No Hit GTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATC 5 0.125 No Hit GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA 5 0.125 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 5 0.125 No Hit CAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTG 5 0.125 No Hit GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA 5 0.125 No Hit CAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTAT 5 0.125 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 5 0.125 No Hit CCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGA 5 0.125 No Hit GTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGG 5 0.125 No Hit CATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAA 5 0.125 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5 0.125 No Hit ATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAG 5 0.125 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 5 0.125 No Hit ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 5 0.125 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 5 0.125 No Hit GCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAAC 5 0.125 No Hit GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT 5 0.125 No Hit GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC 5 0.125 No Hit GGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAG 5 0.125 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.5625 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.7375 0.0 0.0 0.0 0.0 92-93 0.8 0.0 0.0 0.0 0.0 94-95 0.95 0.0 0.0 0.0 0.0 96-97 1.1124999999999998 0.0 0.0 0.0 0.0 98-99 1.3375 0.0 0.0 0.0 0.0 100-101 1.5750000000000002 0.0 0.0 0.0 0.0 102-103 1.9 0.0 0.0 0.0 0.0 104-105 2.425 0.0 0.0 0.0 0.0 106-107 2.7875 0.0 0.0 0.0 0.0 108-109 3.3375 0.0 0.0 0.0 0.0 110-111 3.9375 0.0 0.0 0.0 0.0 112-113 4.5375 0.0 0.0 0.0 0.0 114-115 4.9625 0.0 0.0 0.0 0.0 116-117 5.237500000000001 0.0 0.0 0.0 0.0 118-119 5.887499999999999 0.0 0.0 0.0 0.0 120-121 6.5375 0.0 0.0 0.0 0.0 122-123 7.025 0.0 0.0 0.0 0.0 124-125 7.65 0.0 0.0 0.0 0.0 126-127 8.5625 0.0 0.0 0.0 0.0 128-129 9.275 0.0 0.0 0.0 0.0 130-131 10.1 0.0 0.0 0.0 0.0 132-133 10.9875 0.0 0.0 0.0 0.0 134-135 11.7125 0.0 0.0 0.0 0.0 136-137 12.4375 0.0 0.0 0.0 0.0 138-139 13.3375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAATA 10 0.006830828 145.0 7 GCCCATA 15 1.1411342E-4 145.0 1 TAACAAT 10 0.006830828 145.0 6 CCATAAC 10 0.006830828 145.0 3 TCCAACT 10 0.006830828 145.0 9 AATGATT 10 0.006830828 145.0 145 ACAATAG 10 0.006830828 145.0 8 CATAACA 10 0.006830828 145.0 4 AAAAAAA 120 2.133589E-5 12.083334 65-69 >>END_MODULE SRR5578471 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578471_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08375 33.0 33.0 34.0 31.0 34.0 2 32.161 33.0 33.0 34.0 31.0 34.0 3 32.06225 33.0 33.0 34.0 29.0 34.0 4 32.1015 33.0 33.0 34.0 31.0 34.0 5 32.067 33.0 33.0 34.0 30.0 34.0 6 36.22975 38.0 38.0 38.0 33.0 38.0 7 36.13275 38.0 38.0 38.0 33.0 38.0 8 36.07375 38.0 38.0 38.0 33.0 38.0 9 36.07475 38.0 38.0 38.0 33.0 38.0 10-14 36.014149999999994 38.0 37.8 38.0 32.6 38.0 15-19 35.80329999999999 38.0 37.0 38.0 31.0 38.0 20-24 35.6786 38.0 37.0 38.0 30.2 38.0 25-29 35.51065 38.0 37.0 38.0 29.8 38.0 30-34 35.27905 38.0 37.0 38.0 28.8 38.0 35-39 35.05645 38.0 36.4 38.0 27.8 38.0 40-44 34.84315 38.0 36.0 38.0 27.4 38.0 45-49 34.65925 38.0 35.8 38.0 26.8 38.0 50-54 34.321000000000005 38.0 35.0 38.0 25.0 38.0 55-59 33.976699999999994 38.0 34.2 38.0 21.0 38.0 60-64 33.9382 38.0 34.0 38.0 21.0 38.0 65-69 33.273250000000004 38.0 33.8 38.0 16.0 38.0 70-74 32.70195 38.0 33.0 38.0 15.6 38.0 75-79 32.25815 37.4 32.2 38.0 15.0 38.0 80-84 31.674799999999998 37.0 29.8 38.0 15.0 38.0 85-89 31.197249999999997 37.0 29.0 38.0 14.8 38.0 90-94 30.57685 36.6 27.8 38.0 14.0 38.0 95-99 29.791449999999998 35.8 25.4 38.0 13.0 38.0 100-104 28.736650000000004 35.0 22.6 38.0 4.2 38.0 105-109 28.020249999999997 34.2 16.2 38.0 2.0 38.0 110-114 26.883049999999997 34.0 15.0 38.0 2.0 38.0 115-119 25.74125 33.0 14.8 37.6 2.0 38.0 120-124 24.718799999999998 31.0 13.8 37.0 2.0 38.0 125-129 23.051750000000002 27.8 10.8 36.2 2.0 38.0 130-134 21.83035 25.8 2.0 35.8 2.0 38.0 135-139 20.0027 21.2 2.0 35.0 2.0 38.0 140-144 17.847450000000002 14.2 2.0 34.0 2.0 38.0 145-149 15.648600000000002 6.6 2.0 33.4 2.0 38.0 150-151 11.911249999999999 2.0 2.0 28.0 2.0 36.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25.0 3 10.0 4 11.0 5 8.0 6 10.0 7 8.0 8 10.0 9 11.0 10 7.0 11 15.0 12 24.0 13 30.0 14 29.0 15 39.0 16 40.0 17 43.0 18 40.0 19 45.0 20 51.0 21 59.0 22 61.0 23 73.0 24 88.0 25 110.0 26 125.0 27 143.0 28 181.0 29 183.0 30 209.0 31 252.0 32 299.0 33 329.0 34 390.0 35 460.0 36 451.0 37 131.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.075 22.175 12.125 23.625 2 26.327655310621246 28.481963927855713 24.298597194388776 20.89178356713427 3 21.25846076710955 25.670594133868136 31.035347204813235 22.035597894209076 4 24.918525946352467 31.336174479819505 22.236149410879918 21.509150162948107 5 27.124592629731765 34.62020556530459 19.62897969415894 18.62622211080471 6 24.3 33.15 22.25 20.3 7 20.78019504876219 24.131032758189548 32.75818954738685 22.330582645661416 8 22.575 27.025 25.75 24.65 9 25.025 25.224999999999998 27.125 22.625 10-14 25.055022008803522 27.290916366546618 23.359343737494996 24.294717887154864 15-19 25.66322955250776 25.958554409850837 25.513064370807886 22.865151666833516 20-24 25.919991989185398 27.316877785009762 24.267761477995293 22.495368747809543 25-29 26.364000400440485 27.420162178396236 23.786164781259387 22.429672639903895 30-34 26.34871384245821 26.193574216795117 25.407867080372338 22.04984486037434 35-39 24.73468161794153 25.88105726872247 25.53063676411694 23.853624349219064 40-44 26.683691352461068 25.19152771518702 25.87752240749086 22.24725852486105 45-49 25.227841762643965 24.41161742613921 27.00550826239359 23.355032548823235 50-54 23.95254542724133 25.219001852129953 28.372628522801218 22.4558241978275 55-59 23.752065288138986 25.864917638812397 28.378310719471283 22.00470635357733 60-64 22.76369828708805 27.717119102474207 26.920765301011716 22.598417309426026 65-69 22.8092138207311 29.093640460691038 25.943915873810713 22.15322984476715 70-74 22.92062856570914 28.45060554499049 26.11350215193674 22.51526373736363 75-79 22.398597896845267 28.918377566349523 25.317976965448175 23.365047571357035 80-84 22.737970056582043 28.71663912673376 25.907565970657455 22.63782484602674 85-89 22.609392209872833 28.326824872334033 26.749774707119258 22.314008210673876 90-94 22.84098869208446 28.700090063044133 26.318422896027222 22.14049834884419 95-99 22.801041875375677 29.197555600080143 26.036866359447004 21.964536165097176 100-104 23.336171065150985 28.90981020581902 25.24913616104963 22.50488256798037 105-109 23.737045010764533 29.950433084664297 24.38291693786612 21.929604966705053 110-114 22.443252993936966 29.94438041789848 24.292228290825275 23.32013829733928 115-119 23.539725478408975 30.257489229536116 24.17593427512273 22.026851016932174 120-124 23.524405506883607 30.46808510638298 23.774718397997496 22.23279098873592 125-129 23.527939114760667 30.61285800120168 23.928499899859805 21.93070298417785 130-134 24.243486973947896 29.62424849699399 23.77755511022044 22.354709418837675 135-139 24.14155571128241 30.858944839323254 23.886274902392632 21.113224547001703 140-144 25.051273072882797 29.838427292281526 23.93076884598069 21.179530788854986 145-149 24.843664015208365 29.801390764920704 23.638000900495275 21.716944319375656 150-151 25.647765677806984 30.20403054199524 23.206909500563274 20.941294279634498 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 1.5 12 1.5 13 0.0 14 0.0 15 1.5 16 1.5 17 0.0 18 1.0 19 1.0 20 0.5 21 2.5 22 3.5 23 2.0 24 1.0 25 2.0 26 5.0 27 8.5 28 8.5 29 9.0 30 13.0 31 30.0 32 39.5 33 37.5 34 62.5 35 82.5 36 93.0 37 124.5 38 171.0 39 208.0 40 243.5 41 188.5 42 117.0 43 122.0 44 121.5 45 126.0 46 125.5 47 137.0 48 143.0 49 146.0 50 145.5 51 130.5 52 149.0 53 155.5 54 149.5 55 135.0 56 110.0 57 93.5 58 74.0 59 63.5 60 54.0 61 40.5 62 29.0 63 36.5 64 34.0 65 26.5 66 21.5 67 18.0 68 21.5 69 20.5 70 22.0 71 17.5 72 12.5 73 11.5 74 11.5 75 12.0 76 7.0 77 3.5 78 2.5 79 1.5 80 1.5 81 1.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.2 3 0.27499999999999997 4 0.27499999999999997 5 0.27499999999999997 6 0.0 7 0.025 8 0.0 9 0.0 10-14 0.04 15-19 0.11 20-24 0.135 25-29 0.11 30-34 0.09 35-39 0.12 40-44 0.145 45-49 0.15 50-54 0.11499999999999999 55-59 0.135 60-64 0.16999999999999998 65-69 0.15 70-74 0.09 75-79 0.15 80-84 0.145 85-89 0.13 90-94 0.06999999999999999 95-99 0.18 100-104 0.155 105-109 0.135 110-114 0.215 115-119 0.19 120-124 0.125 125-129 0.13999999999999999 130-134 0.2 135-139 0.11 140-144 0.045 145-149 0.055 150-151 0.13749999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.225 #Duplication Level Percentage of deduplicated Percentage of total 1 88.08467053601912 64.5 2 6.452714236940936 9.45 3 2.4923181973369752 5.475 4 0.7511095937179925 2.1999999999999997 5 0.6145442130419939 2.25 6 0.30727210652099696 1.35 7 0.27313076135199726 1.4000000000000001 8 0.1707067258449983 1.0 9 0.13656538067599863 0.8999999999999999 >10 0.6828269033799932 10.0 >50 0.03414134516899966 1.4749999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 59 1.4749999999999999 Illumina Single End PCR Primer 1 (100% over 50bp) CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 36 0.8999999999999999 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 34 0.8500000000000001 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 32 0.8 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 32 0.8 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 31 0.775 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 22 0.5499999999999999 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 22 0.5499999999999999 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 21 0.525 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 21 0.525 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 19 0.475 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 16 0.4 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 16 0.4 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 15 0.375 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 14 0.35000000000000003 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 13 0.325 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 13 0.325 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 12 0.3 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 11 0.27499999999999997 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 10 0.25 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 10 0.25 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 9 0.22499999999999998 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 9 0.22499999999999998 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 9 0.22499999999999998 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 9 0.22499999999999998 No Hit AATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTT 8 0.2 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 8 0.2 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 8 0.2 No Hit GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG 8 0.2 No Hit AGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCA 8 0.2 No Hit AAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTA 7 0.17500000000000002 No Hit CCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCG 7 0.17500000000000002 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 7 0.17500000000000002 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 7 0.17500000000000002 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 7 0.17500000000000002 No Hit AAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA 7 0.17500000000000002 No Hit TAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAA 7 0.17500000000000002 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 7 0.17500000000000002 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 6 0.15 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 6 0.15 No Hit GCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTT 6 0.15 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 6 0.15 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 6 0.15 No Hit GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG 6 0.15 No Hit GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA 6 0.15 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 6 0.15 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 6 0.15 No Hit TGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCAT 5 0.125 No Hit AAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT 5 0.125 No Hit CTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTCTAA 5 0.125 No Hit AATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGG 5 0.125 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 5 0.125 No Hit CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA 5 0.125 No Hit TATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACT 5 0.125 No Hit GATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCAT 5 0.125 No Hit GGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCA 5 0.125 No Hit AAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTATACCAA 5 0.125 No Hit AGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGT 5 0.125 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 5 0.125 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 5 0.125 No Hit GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT 5 0.125 No Hit GTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCC 5 0.125 No Hit CTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGG 5 0.125 No Hit GATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGT 5 0.125 No Hit TCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.325 0.0 0.0 0.0 0.0 86-87 0.3625 0.0 0.0 0.0 0.0 88-89 0.4 0.0 0.0 0.0 0.0 90-91 0.5249999999999999 0.0 0.0 0.0 0.0 92-93 0.5874999999999999 0.0 0.0 0.0 0.0 94-95 0.725 0.0 0.0 0.0 0.0 96-97 0.875 0.0 0.0 0.0 0.0 98-99 1.0625 0.0 0.0 0.0 0.0 100-101 1.25 0.0 0.0 0.0 0.0 102-103 1.4500000000000002 0.0 0.0 0.0 0.0 104-105 1.7875 0.0 0.0 0.0 0.0 106-107 1.9874999999999998 0.0 0.0 0.0 0.0 108-109 2.3875 0.0 0.0 0.0 0.0 110-111 2.7125000000000004 0.0 0.0 0.0 0.0 112-113 3.0625 0.0 0.0 0.0 0.0 114-115 3.325 0.0 0.0 0.0 0.0 116-117 3.4875 0.0 0.0 0.0 0.0 118-119 3.9125 0.0 0.0 0.0 0.0 120-121 4.3 0.0 0.0 0.0 0.0 122-123 4.5625 0.0 0.0 0.0 0.0 124-125 4.9125 0.0 0.0 0.0 0.0 126-127 5.4625 0.0 0.0 0.0 0.0 128-129 5.875 0.0 0.0 0.0 0.0 130-131 6.324999999999999 0.0 0.0 0.0 0.0 132-133 6.800000000000001 0.0 0.0 0.0 0.0 134-135 7.0875 0.0 0.0 0.0 0.0 136-137 7.4 0.0 0.0 0.0 0.0 138-139 7.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCAAG 10 0.006830828 145.0 1 ATTAGGG 10 0.006830828 145.0 1 GGCTGAA 10 0.006830828 145.0 6 TAGGGCT 10 0.006830828 145.0 3 CAAGCTG 10 0.006830828 145.0 4 TGAAAGC 10 0.006830828 145.0 9 >>END_MODULE Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra Read 770166 spots for SRR5578471.sra Written 770166 spots for SRR5578471.sra SRR ids: ['SRR5578471.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_fz2xi3we SRR5578471.sra spots: 15403320 blocks: [[1, 770166], [770167, 1540332], [1540333, 2310498], [2310499, 3080664], [3080665, 3850830], [3850831, 4620996], [4620997, 5391162], [5391163, 6161328], [6161329, 6931494], [6931495, 7701660], [7701661, 8471826], [8471827, 9241992], [9241993, 10012158], [10012159, 10782324], [10782325, 11552490], [11552491, 12322656], [12322657, 13092822], [13092823, 13862988], [13862989, 14633154], [14633155, 15403320]] SRR5578471 file size 5197979 SRR5578471 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578471 SRR5578471_1.fastq SRR5578471_2.fastq Input file: SRR5578471_1.fastq Paired file: SRR5578471_2.fastq trimmed: SRR5578471-trimmed-pair1.fastq, SRR5578471-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 19:09:56 2024 >> started Mon Dec 9 19:10:14 2024 >> done (17.838s) 15403320 read pairs processed; of these: 53398 ( 0.35%) short read pairs filtered out after trimming by size control 329613 ( 2.14%) empty read pairs filtered out after trimming by size control 15020309 (97.51%) read pairs available; of these: 8574696 (57.09%) trimmed read pairs available after processing 6445613 (42.91%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 17 0.00% 20 23 0.00% 21 28 0.00% 22 30 0.00% 23 52 0.00% 24 78 0.00% 25 48 0.00% 26 53 0.00% 27 64 0.00% 28 52 0.00% 29 50 0.00% 30 77 0.00% 31 90 0.00% 32 77 0.00% 33 52 0.00% 34 78 0.00% 35 101 0.00% 36 73 0.00% 37 78 0.00% 38 88 0.00% 39 81 0.00% 40 98 0.00% 41 120 0.00% 42 144 0.00% 43 170 0.00% 44 192 0.00% 45 229 0.00% 46 284 0.00% 47 299 0.00% 48 304 0.00% 49 366 0.00% 50 360 0.00% 51 413 0.00% 52 504 0.00% 53 486 0.00% 54 627 0.00% 55 696 0.00% 56 708 0.00% 57 762 0.01% 58 844 0.01% 59 754 0.01% 60 916 0.01% 61 947 0.01% 62 1102 0.01% 63 1285 0.01% 64 1625 0.01% 65 2232 0.01% 66 3365 0.02% 67 5362 0.04% 68 10448 0.07% 69 25057 0.17% 70 30750 0.20% 71 13187 0.09% 72 6448 0.04% 73 4879 0.03% 74 4858 0.03% 75 5268 0.04% 76 5304 0.04% 77 5786 0.04% 78 6106 0.04% 79 7052 0.05% 80 7064 0.05% 81 8074 0.05% 82 9571 0.06% 83 10499 0.07% 84 13958 0.09% 85 16967 0.11% 86 17663 0.12% 87 19210 0.13% 88 20496 0.14% 89 21188 0.14% 90 22210 0.15% 91 22043 0.15% 92 23497 0.16% 93 24973 0.17% 94 25423 0.17% 95 26715 0.18% 96 27873 0.19% 97 29366 0.20% 98 30519 0.20% 99 32127 0.21% 100 34633 0.23% 101 36090 0.24% 102 37186 0.25% 103 40516 0.27% 104 41684 0.28% 105 44355 0.30% 106 46645 0.31% 107 47801 0.32% 108 50634 0.34% 109 49158 0.33% 110 50711 0.34% 111 53741 0.36% 112 57728 0.38% 113 63155 0.42% 114 67033 0.45% 115 69218 0.46% 116 67956 0.45% 117 69079 0.46% 118 68627 0.46% 119 69895 0.47% 120 74639 0.50% 121 76377 0.51% 122 80296 0.53% 123 83642 0.56% 124 86769 0.58% 125 88823 0.59% 126 92173 0.61% 127 91282 0.61% 128 88585 0.59% 129 97294 0.65% 130 96551 0.64% 131 98661 0.66% 132 101252 0.67% 133 105961 0.71% 134 109127 0.73% 135 110440 0.74% 136 112193 0.75% 137 115202 0.77% 138 123057 0.82% 139 128043 0.85% 140 133069 0.89% 141 137030 0.91% 142 159877 1.06% 143 166215 1.11% 144 177158 1.18% 145 202536 1.35% 146 231501 1.54% 147 284355 1.89% 148 388520 2.59% 149 639841 4.26% 150 2467309 16.43% 151 6445613 42.91% 15020309 reads passed initial QC criterion=sequence-density sequence-density=0.63 sequence-density-rank=1 fanout-score=21.92 fanout-score-rank=4 prefix-density=7.11 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=48.44 fanout-score-rank=1 prefix-density=0.58 prefix-fanout=1.3 sequence=TTTTTTTTCTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATTCCCGAGGCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCCCAGAGACGGGCGGAGGCTCTTAACCGTACGAGACAATCTCC criterion=sequence-density sequence-density=0.97 sequence-density-rank=1 fanout-score=4.86 fanout-score-rank=10 prefix-density=4.67 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.16 sequence-density-rank=18 fanout-score=76.88 fanout-score-rank=1 prefix-density=12.27 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR5578471 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 19:12:09 Started mapping on | Dec 09 19:12:09 Finished on | Dec 09 19:45:07 Mapping speed, Million of reads per hour | 27.34 Number of input reads | 15020309 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 5192507 Uniquely mapped reads % | 34.57% Average mapped length | 281.97 Number of splices: Total | 3659692 Number of splices: Annotated (sjdb) | 3426850 Number of splices: GT/AG | 3616718 Number of splices: GC/AG | 35318 Number of splices: AT/AC | 1379 Number of splices: Non-canonical | 6277 Mismatch rate per base, % | 0.16% Deletion rate per base | 0.01% Deletion average length | 1.40 Insertion rate per base | 0.01% Insertion average length | 1.28 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 169830 % of reads mapped to multiple loci | 1.13% Number of reads mapped to too many loci | 43385 % of reads mapped to too many loci | 0.29% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 62.92% % of reads unmapped: other | 1.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9672474 9672474 9672474 N_multimapping 169830 169830 169830 N_noFeature 205723 4975490 281134 N_ambiguous 165043 526 23955 UnstrandedReadsAssigned:4821741 PositiveStrandReadsAssigned:216491 NegativeStrandReadsAssigned:4887418 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=142 echo kmer=137 SRR5578471 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578471-trimmed-pair1.fastq SRR5578471-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,020,309 reads, 4,950,868 reads pseudoaligned [quant] estimated average fragment length: 199.031 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,060 rounds 52973 SRR5578471.ke.tsv 35125 SRR5578471.se.tsv 88098 total ==> SRR5578471.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 738.225 1.37999e-08 3.88442e-09 PNS24247 1044 845.969 0 0 PNS24249 1928 1729.97 46.8336 5.62547 PNS24246 1044 845.969 0 0 PNS24248 1044 845.969 0 0 PNS24244 1471 1272.97 148.166 24.1864 PNS24243 293 117.976 0 0 KQK14069 1603 1404.97 299.138 44.243 KQK14071 474 280.953 0 0 ==> SRR5578471.se.tsv <== BRADI_1g14170v3 310 BRADI_1g53295v3 9 BRADI_1g59795v3 42 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 67 BRADI_1g74790v3 397 BRADI_1g09890v3 0 BRADI_1g77505v3 42 BRADI_1g48960v3 0 SRR5578471 completed mapping pipeline successfully