Starting /dee2/code/volunteer_pipeline.sh SRR5578473 current disk space = 1522352197632 free memory = 1569717696 SRR5578473 SRAfilesize 84ce99f2e5ed9e72ae3e2b132ad58f68 SRR5578473.sra SRR5578473.sra file validated SRR5578473 is paired end SRR5578473 is conventional basespace SRR5578473 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578473_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.16725 34.0 33.0 34.0 33.0 34.0 2 33.4135 34.0 34.0 34.0 33.0 34.0 3 33.44275 34.0 34.0 34.0 33.0 34.0 4 33.42425 34.0 34.0 34.0 33.0 34.0 5 33.423 34.0 34.0 34.0 33.0 34.0 6 37.1295 38.0 38.0 38.0 36.0 38.0 7 37.34825 38.0 38.0 38.0 37.0 38.0 8 37.422 38.0 38.0 38.0 37.0 38.0 9 37.45 38.0 38.0 38.0 38.0 38.0 10-14 37.4128 38.0 38.0 38.0 37.0 38.0 15-19 37.4553 38.0 38.0 38.0 37.6 38.0 20-24 37.445800000000006 38.0 38.0 38.0 37.6 38.0 25-29 37.35255 38.0 38.0 38.0 37.8 38.0 30-34 37.3531 38.0 38.0 38.0 37.2 38.0 35-39 37.2813 38.0 38.0 38.0 37.0 38.0 40-44 37.1751 38.0 38.0 38.0 37.0 38.0 45-49 37.16155 38.0 38.0 38.0 37.0 38.0 50-54 37.182249999999996 38.0 38.0 38.0 37.0 38.0 55-59 37.0976 38.0 38.0 38.0 36.8 38.0 60-64 37.069449999999996 38.0 38.0 38.0 36.6 38.0 65-69 37.035 38.0 38.0 38.0 36.0 38.0 70-74 36.8792 38.0 38.0 38.0 36.0 38.0 75-79 36.5817 38.0 38.0 38.0 35.8 38.0 80-84 36.488299999999995 38.0 38.0 38.0 35.4 38.0 85-89 36.45 38.0 38.0 38.0 35.0 38.0 90-94 36.31125 38.0 38.0 38.0 34.6 38.0 95-99 36.277300000000004 38.0 38.0 38.0 34.4 38.0 100-104 36.15665 38.0 38.0 38.0 34.0 38.0 105-109 36.047450000000005 38.0 38.0 38.0 34.0 38.0 110-114 35.9134 38.0 38.0 38.0 33.6 38.0 115-119 35.8105 38.0 38.0 38.0 33.4 38.0 120-124 35.69839999999999 38.0 38.0 38.0 32.8 38.0 125-129 35.493449999999996 38.0 37.6 38.0 31.8 38.0 130-134 35.3634 38.0 37.2 38.0 31.2 38.0 135-139 35.19845 38.0 36.2 38.0 31.0 38.0 140-144 35.0173 38.0 36.0 38.0 30.4 38.0 145-149 34.38925 38.0 35.8 38.0 27.4 38.0 150-151 31.260624999999997 36.5 31.5 38.0 12.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 2.0 6 4.0 7 3.0 8 2.0 9 1.0 10 0.0 11 2.0 12 1.0 13 1.0 14 4.0 15 5.0 16 4.0 17 3.0 18 18.0 19 26.0 20 6.0 21 3.0 22 12.0 23 4.0 24 6.0 25 11.0 26 19.0 27 16.0 28 20.0 29 22.0 30 37.0 31 40.0 32 62.0 33 59.0 34 93.0 35 172.0 36 433.0 37 2908.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.561933534743204 11.706948640483382 11.027190332326283 32.703927492447136 2 27.6 12.425 29.275000000000002 30.7 3 23.175 17.1 25.025 34.699999999999996 4 26.400000000000002 24.175 23.974999999999998 25.45 5 27.250000000000004 26.85 25.95 19.950000000000003 6 23.549999999999997 31.075000000000003 27.150000000000002 18.224999999999998 7 16.35408852213053 24.5311327831958 41.5103775943986 17.60440110027507 8 19.25 26.625 31.474999999999998 22.650000000000002 9 20.125 23.75 35.175 20.95 10-14 23.185 28.595 25.835 22.384999999999998 15-19 22.41 26.395000000000003 27.075 24.12 20-24 23.285 25.945 27.034999999999997 23.735 25-29 21.97 27.22 28.07 22.74 30-34 21.77 27.389999999999997 27.08 23.76 35-39 22.36 26.305 27.944999999999997 23.39 40-44 23.44 25.915 26.825 23.82 45-49 23.635 26.96 27.339999999999996 22.065 50-54 24.12 26.700000000000003 25.905 23.275000000000002 55-59 23.255 27.01 26.52 23.215 60-64 21.959999999999997 27.725 26.200000000000003 24.115000000000002 65-69 21.705 28.555000000000003 25.919999999999998 23.82 70-74 22.93 27.87 25.5 23.7 75-79 22.475 26.575 26.724999999999998 24.224999999999998 80-84 23.585 27.189999999999998 25.685000000000002 23.54 85-89 23.84 26.779999999999998 25.900000000000002 23.48 90-94 23.62 25.94 26.484999999999996 23.955000000000002 95-99 22.855 25.555 27.095000000000002 24.495 100-104 23.0 26.965 26.400000000000002 23.635 105-109 22.785 27.779999999999998 26.025 23.41 110-114 22.865 26.455000000000002 25.105 25.575 115-119 22.173325998899834 27.169075361304195 26.428964344651696 24.22863429514427 120-124 23.07 26.76 24.63 25.540000000000003 125-129 23.165 27.62 24.529999999999998 24.685000000000002 130-134 23.630000000000003 26.69 25.285000000000004 24.395 135-139 22.45 27.750000000000004 26.14 23.66 140-144 23.830000000000002 27.305 25.15 23.715 145-149 22.685 28.860000000000003 23.419999999999998 25.035 150-151 22.655663915978995 26.231557889472366 24.93123280820205 26.18154538634659 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 2.0 7 3.0 8 1.5 9 0.5 10 0.5 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.5 22 2.0 23 1.0 24 1.0 25 2.0 26 2.5 27 2.0 28 3.0 29 18.0 30 28.5 31 48.5 32 62.5 33 58.5 34 74.5 35 95.0 36 119.5 37 156.0 38 160.0 39 122.0 40 135.0 41 142.5 42 112.5 43 112.0 44 118.5 45 131.5 46 150.5 47 149.5 48 154.0 49 172.5 50 168.0 51 162.0 52 158.0 53 156.5 54 146.0 55 122.0 56 98.5 57 82.5 58 76.5 59 68.0 60 60.0 61 47.0 62 35.0 63 38.5 64 37.5 65 28.5 66 25.5 67 26.0 68 23.5 69 18.5 70 18.0 71 13.0 72 8.5 73 8.5 74 6.5 75 4.5 76 5.0 77 3.5 78 1.0 79 2.0 80 2.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7000000000000001 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.025 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.015 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.0 #Duplication Level Percentage of deduplicated Percentage of total 1 87.66447368421053 66.625 2 6.578947368421052 10.0 3 2.598684210526316 5.925 4 0.9868421052631579 3.0 5 0.9868421052631579 3.75 6 0.3618421052631579 1.6500000000000001 7 0.13157894736842105 0.7000000000000001 8 0.1644736842105263 1.0 9 0.09868421052631579 0.675 >10 0.4276315789473685 6.675000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTAATCTCGTATGC 46 1.15 TruSeq Adapter, Index 18 (97% over 37bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 43 1.075 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 32 0.8 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 22 0.5499999999999999 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 19 0.475 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 17 0.42500000000000004 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 16 0.4 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 15 0.375 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 14 0.35000000000000003 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 13 0.325 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 10 0.25 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 10 0.25 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 10 0.25 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 9 0.22499999999999998 No Hit GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA 9 0.22499999999999998 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 9 0.22499999999999998 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 8 0.2 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 8 0.2 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 8 0.2 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 8 0.2 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 8 0.2 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 7 0.17500000000000002 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 7 0.17500000000000002 No Hit GGCCCACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGT 7 0.17500000000000002 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 7 0.17500000000000002 No Hit CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT 6 0.15 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 6 0.15 No Hit CAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTG 6 0.15 No Hit GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCC 6 0.15 No Hit ATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATA 6 0.15 No Hit GCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACATATGCAGAT 6 0.15 No Hit GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG 6 0.15 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 6 0.15 No Hit CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA 6 0.15 No Hit TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT 6 0.15 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 6 0.15 No Hit CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC 5 0.125 No Hit ACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAAT 5 0.125 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 5 0.125 No Hit GTCCAAAGTTAAGCTCTGCACCTGTTACACTATAGTCATCGTCCATAACA 5 0.125 No Hit GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT 5 0.125 No Hit CCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAA 5 0.125 No Hit GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA 5 0.125 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 5 0.125 No Hit GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT 5 0.125 No Hit TACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAA 5 0.125 No Hit TGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTT 5 0.125 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 5 0.125 No Hit GCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAAT 5 0.125 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 5 0.125 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 5 0.125 No Hit CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC 5 0.125 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 5 0.125 No Hit AGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCG 5 0.125 No Hit CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT 5 0.125 No Hit TGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATAT 5 0.125 No Hit ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 5 0.125 No Hit GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA 5 0.125 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 5 0.125 No Hit GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT 5 0.125 No Hit CGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAAC 5 0.125 No Hit CCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATG 5 0.125 No Hit GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC 5 0.125 No Hit CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA 5 0.125 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 5 0.125 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.425 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.7125 0.0 0.0 0.0 0.0 94-95 0.8875 0.0 0.0 0.0 0.0 96-97 1.025 0.0 0.0 0.0 0.0 98-99 1.1625 0.0 0.0 0.0 0.0 100-101 1.35 0.0 0.0 0.0 0.0 102-103 1.5125000000000002 0.0 0.0 0.0 0.0 104-105 1.75 0.0 0.0 0.0 0.0 106-107 2.2875 0.0 0.0 0.0 0.0 108-109 2.5999999999999996 0.0 0.0 0.0 0.0 110-111 2.9 0.0 0.0 0.0 0.0 112-113 3.35 0.0 0.0 0.0 0.0 114-115 3.9875000000000003 0.0 0.0 0.0 0.0 116-117 4.5875 0.0 0.0 0.0 0.0 118-119 5.1625 0.0 0.0 0.0 0.0 120-121 5.9625 0.0 0.0 0.0 0.0 122-123 6.550000000000001 0.0 0.0 0.0 0.0 124-125 7.1375 0.0 0.0 0.0 0.0 126-127 7.9875 0.0 0.0 0.0 0.0 128-129 8.7375 0.0 0.0 0.0 0.0 130-131 9.4125 0.0 0.0 0.0 0.0 132-133 10.1625 0.0 0.0 0.0 0.0 134-135 10.912500000000001 0.0 0.0 0.0 0.0 136-137 11.787500000000001 0.0 0.0 0.0 0.0 138-139 12.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATGGA 10 0.006830828 145.0 7 ATGGATA 10 0.006830828 145.0 9 CCTCACT 10 0.006830828 145.0 145 TTTATGG 10 0.006830828 145.0 6 AGTTTTA 10 0.006830828 145.0 3 TCCAGTC 45 0.008957279 48.333332 145 >>END_MODULE SRR5578473 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578473_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89275 33.0 32.0 34.0 28.0 34.0 2 32.06225 33.0 33.0 34.0 31.0 34.0 3 31.999 33.0 33.0 34.0 29.0 34.0 4 31.983 33.0 33.0 34.0 31.0 34.0 5 31.933 33.0 33.0 34.0 30.0 34.0 6 36.04975 38.0 38.0 38.0 33.0 38.0 7 35.8875 38.0 37.0 38.0 31.0 38.0 8 35.9885 38.0 38.0 38.0 32.0 38.0 9 36.00275 38.0 38.0 38.0 31.0 38.0 10-14 35.8016 38.0 37.0 38.0 30.6 38.0 15-19 35.674549999999996 38.0 37.0 38.0 30.2 38.0 20-24 35.6697 38.0 37.0 38.0 30.0 38.0 25-29 35.46205 38.0 37.0 38.0 29.0 38.0 30-34 35.1765 38.0 36.4 38.0 28.4 38.0 35-39 35.012 38.0 36.0 38.0 27.2 38.0 40-44 34.838800000000006 38.0 36.0 38.0 27.0 38.0 45-49 34.679050000000004 38.0 36.0 38.0 26.6 38.0 50-54 34.413599999999995 38.0 35.0 38.0 25.4 38.0 55-59 34.18365 38.0 35.0 38.0 23.2 38.0 60-64 33.993700000000004 38.0 34.2 38.0 22.6 38.0 65-69 33.4991 38.0 34.0 38.0 16.0 38.0 70-74 32.944849999999995 38.0 33.2 38.0 16.0 38.0 75-79 32.6503 37.8 33.0 38.0 15.4 38.0 80-84 32.24235 37.0 31.4 38.0 15.0 38.0 85-89 31.6478 37.0 29.4 38.0 15.0 38.0 90-94 30.971799999999995 36.4 28.4 38.0 14.8 38.0 95-99 30.34345 35.8 26.4 38.0 13.4 38.0 100-104 29.529650000000004 35.0 24.0 38.0 13.0 38.0 105-109 28.532300000000003 34.8 21.8 38.0 6.4 38.0 110-114 27.671000000000003 34.2 17.4 38.0 2.0 38.0 115-119 26.6649 33.8 15.0 38.0 2.0 38.0 120-124 25.63605 33.2 14.4 37.6 2.0 38.0 125-129 24.45585 31.0 13.4 37.0 2.0 38.0 130-134 22.786849999999998 28.4 6.4 36.0 2.0 38.0 135-139 21.103 24.0 2.0 35.4 2.0 38.0 140-144 19.14375 20.2 2.0 34.6 2.0 38.0 145-149 16.796650000000003 8.8 2.0 33.6 2.0 38.0 150-151 12.23575 2.0 2.0 27.5 2.0 35.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24.0 3 9.0 4 9.0 5 9.0 6 5.0 7 12.0 8 5.0 9 5.0 10 16.0 11 9.0 12 14.0 13 18.0 14 31.0 15 42.0 16 32.0 17 45.0 18 29.0 19 42.0 20 42.0 21 60.0 22 76.0 23 78.0 24 95.0 25 85.0 26 106.0 27 146.0 28 176.0 29 159.0 30 202.0 31 262.0 32 296.0 33 324.0 34 412.0 35 453.0 36 468.0 37 204.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.6 19.6 13.5 29.299999999999997 2 27.88485607008761 26.758448060075096 25.081351689612013 20.275344180225282 3 23.654568210262827 26.533166458072593 27.63454317897372 22.177722152690862 4 26.48310387984981 31.33917396745932 21.051314142678347 21.126408010012515 5 27.108886107634543 32.49061326658323 20.550688360450565 19.849812265331664 6 23.84884884884885 33.50850850850851 22.17217217217217 20.47047047047047 7 21.6270337922403 22.95369211514393 33.166458072590736 22.252816020025033 8 21.8523153942428 25.3566958698373 25.5819774718398 27.2090112640801 9 24.23028785982478 24.28035043804756 27.15894868585732 24.330413016270338 10-14 25.54192740926158 26.39299123904881 23.143929912390487 24.921151439299123 15-19 25.58209403635271 26.47839367082269 24.800961394021332 23.138550898803263 20-24 25.53830746119179 26.855282924386582 24.111166750125186 23.495242864296443 25-29 25.658487731597397 26.82523785678518 24.271407110665997 23.244867300951427 30-34 26.772836538461537 26.156850961538463 24.303886217948715 22.766426282051285 35-39 26.030347037908758 25.55460964494967 25.06384896589714 23.351194351244427 40-44 26.987782896054473 25.355497696775487 24.924894852793912 22.731824554376125 45-49 25.96635289405167 24.714600440616863 26.031444021630286 23.287602643701184 50-54 24.839775685960344 24.374123773282598 27.403364710594836 23.382735830162225 55-59 24.225126433328327 26.007711181212755 27.71017976065295 22.05698262480597 60-64 23.54531797696545 25.98397596394592 27.200801201802705 23.26990485728593 65-69 23.471380639991988 27.59276879162702 25.920176273223493 23.015674295157492 70-74 22.8743114672008 27.090635953930896 25.733600400600903 24.301452178267404 75-79 22.544944664229554 27.71295508037458 25.489508738544743 24.25259151685112 80-84 23.51144273624117 27.02689168210727 26.185587660874354 23.276077920777205 85-89 22.675146477039412 28.278832189894338 26.14552556462517 22.900495768441083 90-94 23.520280420630947 28.177265898848276 25.81872809213821 22.483725588382576 95-99 22.6578538881378 28.821791597816837 26.102849131240298 22.417505382805068 100-104 23.99979970957889 28.50132692403986 24.831004957187922 22.667868409193332 105-109 23.43249198717949 28.740985576923077 25.33553685897436 22.490985576923077 110-114 23.367387820512818 29.056490384615387 23.908253205128204 23.66786858974359 115-119 23.415122684026038 29.664496745117674 24.071106659989987 22.849273910866298 120-124 23.69646882043576 29.381417480591033 23.75657400450789 23.165539694465316 125-129 23.920881321982975 30.215322984476717 23.360040060090135 22.503755633450176 130-134 24.261392088132197 28.5728592889334 24.42163244867301 22.744116174261393 135-139 24.747070019032353 29.23970750275468 24.085946108384253 21.927276369828707 140-144 25.86880320480721 28.913370055082627 24.061091637456183 21.156735102653982 145-149 26.262828535669584 28.270337922403005 23.779724655819777 21.687108886107634 150-151 26.107634543178975 28.735919899874844 23.85481852315394 21.30162703379224 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 2.5 2 2.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 1.5 19 2.0 20 2.5 21 2.0 22 2.5 23 3.0 24 1.5 25 1.5 26 3.5 27 5.0 28 8.0 29 14.5 30 16.5 31 21.5 32 30.5 33 38.0 34 47.0 35 63.0 36 85.0 37 122.5 38 161.5 39 165.5 40 199.5 41 166.5 42 95.0 43 108.0 44 113.5 45 113.0 46 123.5 47 136.5 48 157.5 49 158.0 50 133.0 51 140.5 52 161.0 53 164.0 54 174.0 55 170.5 56 138.5 57 114.0 58 100.5 59 84.5 60 70.5 61 52.0 62 36.0 63 33.5 64 33.5 65 34.5 66 32.0 67 24.0 68 18.5 69 18.5 70 17.0 71 11.5 72 10.0 73 10.0 74 8.5 75 9.5 76 9.0 77 4.5 78 2.0 79 1.5 80 2.0 81 1.5 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.125 4 0.125 5 0.125 6 0.1 7 0.125 8 0.125 9 0.125 10-14 0.125 15-19 0.145 20-24 0.15 25-29 0.15 30-34 0.16 35-39 0.155 40-44 0.13999999999999999 45-49 0.13999999999999999 50-54 0.13999999999999999 55-59 0.145 60-64 0.15 65-69 0.155 70-74 0.15 75-79 0.155 80-84 0.155 85-89 0.155 90-94 0.15 95-99 0.145 100-104 0.145 105-109 0.16 110-114 0.16 115-119 0.15 120-124 0.17500000000000002 125-129 0.15 130-134 0.15 135-139 0.16999999999999998 140-144 0.15 145-149 0.125 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.94999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 88.80842659644503 67.45 2 6.352863726135616 9.65 3 1.6458196181698488 3.75 4 1.0204081632653061 3.1 5 0.4279131007241606 1.625 6 0.4937458854509546 2.25 7 0.2304147465437788 1.225 8 0.16458196181698487 1.0 9 0.16458196181698487 1.125 >10 0.6912442396313364 8.825 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 30 0.75 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 28 0.7000000000000001 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 23 0.575 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 22 0.5499999999999999 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 22 0.5499999999999999 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 22 0.5499999999999999 Illumina Single End PCR Primer 1 (100% over 50bp) GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 19 0.475 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 19 0.475 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 18 0.44999999999999996 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 16 0.4 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 15 0.375 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 14 0.35000000000000003 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 13 0.325 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 13 0.325 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 13 0.325 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 12 0.3 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 12 0.3 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 11 0.27499999999999997 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 11 0.27499999999999997 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 10 0.25 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 10 0.25 No Hit CAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCG 9 0.22499999999999998 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 9 0.22499999999999998 No Hit CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC 9 0.22499999999999998 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 9 0.22499999999999998 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 9 0.22499999999999998 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 8 0.2 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 8 0.2 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 8 0.2 No Hit GACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCA 8 0.2 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 8 0.2 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 7 0.17500000000000002 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 7 0.17500000000000002 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 7 0.17500000000000002 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 7 0.17500000000000002 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 7 0.17500000000000002 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 7 0.17500000000000002 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 7 0.17500000000000002 No Hit CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA 6 0.15 No Hit CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT 6 0.15 No Hit GGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC 6 0.15 No Hit GGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTC 6 0.15 No Hit GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG 6 0.15 No Hit AGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGA 6 0.15 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 6 0.15 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 6 0.15 No Hit CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA 6 0.15 No Hit CTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATC 6 0.15 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 6 0.15 No Hit TTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGG 6 0.15 No Hit CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG 6 0.15 No Hit CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA 6 0.15 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 6 0.15 No Hit TGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACA 5 0.125 No Hit CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA 5 0.125 No Hit CCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCG 5 0.125 No Hit GGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCA 5 0.125 No Hit GGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTT 5 0.125 No Hit GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA 5 0.125 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 5 0.125 No Hit CAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTC 5 0.125 No Hit CTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTT 5 0.125 No Hit ACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAG 5 0.125 No Hit GGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTC 5 0.125 No Hit AGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCA 5 0.125 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.32499999999999996 0.0 0.0 0.0 0.0 86-87 0.4 0.0 0.0 0.0 0.0 88-89 0.5125 0.0 0.0 0.0 0.0 90-91 0.55 0.0 0.0 0.0 0.0 92-93 0.6499999999999999 0.0 0.0 0.0 0.0 94-95 0.8375 0.0 0.0 0.0 0.0 96-97 0.975 0.0 0.0 0.0 0.0 98-99 1.0625 0.0 0.0 0.0 0.0 100-101 1.225 0.0 0.0 0.0 0.0 102-103 1.3375 0.0 0.0 0.0 0.0 104-105 1.525 0.0 0.0 0.0 0.0 106-107 1.9125 0.0 0.0 0.0 0.0 108-109 2.1375 0.0 0.0 0.0 0.0 110-111 2.2875 0.0 0.0 0.0 0.0 112-113 2.6125 0.0 0.0 0.0 0.0 114-115 3.0875000000000004 0.0 0.0 0.0 0.0 116-117 3.5125 0.0 0.0 0.0 0.0 118-119 3.8875 0.0 0.0 0.0 0.0 120-121 4.2625 0.0 0.0 0.0 0.0 122-123 4.5625 0.0 0.0 0.0 0.0 124-125 4.9 0.0 0.0 0.0 0.0 126-127 5.45 0.0 0.0 0.0 0.0 128-129 5.9625 0.0 0.0 0.0 0.0 130-131 6.35 0.0 0.0 0.0 0.0 132-133 6.725 0.0 0.0 0.0 0.0 134-135 7.1375 0.0 0.0 0.0 0.0 136-137 7.575 0.0 0.0 0.0 0.0 138-139 8.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATAC 10 0.006830828 145.0 1 ACTTCCC 10 0.006830828 145.0 6 TTCCCAA 10 0.006830828 145.0 8 TCATACT 10 0.006830828 145.0 2 >>END_MODULE Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949053 spots for SRR5578473.sra Written 949053 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra Read 949041 spots for SRR5578473.sra Written 949041 spots for SRR5578473.sra SRR ids: ['SRR5578473.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6g_ucp6l SRR5578473.sra spots: 18980832 blocks: [[1, 949041], [949042, 1898082], [1898083, 2847123], [2847124, 3796164], [3796165, 4745205], [4745206, 5694246], [5694247, 6643287], [6643288, 7592328], [7592329, 8541369], [8541370, 9490410], [9490411, 10439451], [10439452, 11388492], [11388493, 12337533], [12337534, 13286574], [13286575, 14235615], [14235616, 15184656], [15184657, 16133697], [16133698, 17082738], [17082739, 18031779], [18031780, 18980832]] SRR5578473 file size 6410280 SRR5578473 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578473 SRR5578473_1.fastq SRR5578473_2.fastq Input file: SRR5578473_1.fastq Paired file: SRR5578473_2.fastq trimmed: SRR5578473-trimmed-pair1.fastq, SRR5578473-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 19:32:04 2024 >> started Mon Dec 9 19:32:27 2024 >> done (23.311s) 18980832 read pairs processed; of these: 50658 ( 0.27%) short read pairs filtered out after trimming by size control 225745 ( 1.19%) empty read pairs filtered out after trimming by size control 18704429 (98.54%) read pairs available; of these: 10033682 (53.64%) trimmed read pairs available after processing 8670747 (46.36%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 16 0.00% 20 13 0.00% 21 14 0.00% 22 30 0.00% 23 30 0.00% 24 40 0.00% 25 35 0.00% 26 20 0.00% 27 38 0.00% 28 42 0.00% 29 31 0.00% 30 31 0.00% 31 42 0.00% 32 41 0.00% 33 27 0.00% 34 42 0.00% 35 64 0.00% 36 43 0.00% 37 57 0.00% 38 59 0.00% 39 54 0.00% 40 93 0.00% 41 62 0.00% 42 69 0.00% 43 106 0.00% 44 109 0.00% 45 164 0.00% 46 180 0.00% 47 197 0.00% 48 178 0.00% 49 231 0.00% 50 248 0.00% 51 279 0.00% 52 320 0.00% 53 347 0.00% 54 399 0.00% 55 435 0.00% 56 453 0.00% 57 539 0.00% 58 597 0.00% 59 614 0.00% 60 662 0.00% 61 690 0.00% 62 869 0.00% 63 893 0.00% 64 1090 0.01% 65 1470 0.01% 66 1765 0.01% 67 2493 0.01% 68 5016 0.03% 69 10313 0.06% 70 10640 0.06% 71 5700 0.03% 72 3740 0.02% 73 3463 0.02% 74 3598 0.02% 75 4059 0.02% 76 4281 0.02% 77 4881 0.03% 78 5230 0.03% 79 5902 0.03% 80 6367 0.03% 81 7181 0.04% 82 8544 0.05% 83 9629 0.05% 84 12848 0.07% 85 15257 0.08% 86 16426 0.09% 87 19123 0.10% 88 20586 0.11% 89 21220 0.11% 90 21982 0.12% 91 21694 0.12% 92 23190 0.12% 93 24172 0.13% 94 25277 0.14% 95 26751 0.14% 96 28490 0.15% 97 29741 0.16% 98 30780 0.16% 99 33426 0.18% 100 35479 0.19% 101 37325 0.20% 102 38723 0.21% 103 41126 0.22% 104 43142 0.23% 105 46313 0.25% 106 49062 0.26% 107 51125 0.27% 108 53289 0.28% 109 53062 0.28% 110 54725 0.29% 111 56927 0.30% 112 60993 0.33% 113 67229 0.36% 114 71945 0.38% 115 76042 0.41% 116 76143 0.41% 117 76309 0.41% 118 77635 0.42% 119 77044 0.41% 120 81676 0.44% 121 83245 0.45% 122 88063 0.47% 123 91302 0.49% 124 94617 0.51% 125 96279 0.51% 126 101717 0.54% 127 103655 0.55% 128 102175 0.55% 129 108289 0.58% 130 109201 0.58% 131 111870 0.60% 132 114759 0.61% 133 119013 0.64% 134 121633 0.65% 135 124875 0.67% 136 128049 0.68% 137 132589 0.71% 138 138420 0.74% 139 146446 0.78% 140 154526 0.83% 141 158826 0.85% 142 180172 0.96% 143 188124 1.01% 144 206330 1.10% 145 237085 1.27% 146 271936 1.45% 147 339256 1.81% 148 458733 2.45% 149 797473 4.26% 150 3213871 17.18% 151 8670747 46.36% 18704429 reads passed initial QC criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=21.79 fanout-score-rank=3 prefix-density=7.24 prefix-fanout=2.0 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=72.19 fanout-score-rank=1 prefix-density=0.60 prefix-fanout=1.3 sequence=TTTTTTTTCTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATTCCCGAGGCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCCCAGAGACGGGCGGAGGCTCTTAACCGTACGAGACAATCTCCA criterion=sequence-density sequence-density=1.05 sequence-density-rank=1 fanout-score=4.79 fanout-score-rank=11 prefix-density=4.97 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.15 sequence-density-rank=13 fanout-score=78.47 fanout-score-rank=1 prefix-density=11.51 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR5578473 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 19:34:20 Started mapping on | Dec 09 19:34:20 Finished on | Dec 09 20:11:47 Mapping speed, Million of reads per hour | 29.97 Number of input reads | 18704429 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 6572177 Uniquely mapped reads % | 35.14% Average mapped length | 284.97 Number of splices: Total | 5089590 Number of splices: Annotated (sjdb) | 4787840 Number of splices: GT/AG | 5019093 Number of splices: GC/AG | 64301 Number of splices: AT/AC | 2051 Number of splices: Non-canonical | 4145 Mismatch rate per base, % | 0.14% Deletion rate per base | 0.00% Deletion average length | 1.35 Insertion rate per base | 0.00% Insertion average length | 1.21 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 141012 % of reads mapped to multiple loci | 0.75% Number of reads mapped to too many loci | 56312 % of reads mapped to too many loci | 0.30% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 62.76% % of reads unmapped: other | 1.05% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 12003793 12003793 12003793 N_multimapping 141012 141012 141012 N_noFeature 200273 6320905 271043 N_ambiguous 207251 584 27913 UnstrandedReadsAssigned:6164653 PositiveStrandReadsAssigned:250688 NegativeStrandReadsAssigned:6273221 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=146 echo kmer=141 SRR5578473 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578473-trimmed-pair1.fastq SRR5578473-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,704,429 reads, 6,322,589 reads pseudoaligned [quant] estimated average fragment length: 207.529 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,018 rounds 52973 SRR5578473.ke.tsv 35125 SRR5578473.se.tsv 88098 total ==> SRR5578473.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 729.677 44.724 10.3668 PNS24247 1044 837.471 3.12899 0.631931 PNS24249 1928 1721.47 22.6014 2.2206 PNS24246 1044 837.471 3.12899 0.631931 PNS24248 1044 837.471 3.12899 0.631931 PNS24244 1471 1264.47 37.2876 4.98759 PNS24243 293 113.344 0 0 KQK14069 1603 1396.47 930.944 112.753 KQK14071 474 273.031 18.9807 11.7581 ==> SRR5578473.se.tsv <== BRADI_1g14170v3 962 BRADI_1g53295v3 2 BRADI_1g59795v3 77 BRADI_1g07683v3 0 BRADI_1g00485v3 11 BRADI_1g20270v3 141 BRADI_1g74790v3 268 BRADI_1g09890v3 3 BRADI_1g77505v3 85 BRADI_1g48960v3 0 SRR5578473 completed mapping pipeline successfully