Starting /dee2/code/volunteer_pipeline.sh SRR5578482 current disk space = 1521279410176 free memory = 1568056692 SRR5578482 SRAfilesize d8578fc04217caa7a10fa64fb99a40fb SRR5578482.sra SRR5578482.sra file validated SRR5578482 is paired end SRR5578482 is conventional basespace SRR5578482 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578482_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.485 34.0 33.0 34.0 32.0 34.0 2 33.3145 34.0 34.0 34.0 33.0 34.0 3 33.393 34.0 34.0 34.0 33.0 34.0 4 33.4565 34.0 34.0 34.0 33.0 34.0 5 33.47925 34.0 34.0 34.0 33.0 34.0 6 36.997 38.0 37.0 38.0 36.0 38.0 7 37.356 38.0 38.0 38.0 37.0 38.0 8 37.485 38.0 38.0 38.0 37.0 38.0 9 37.54775 38.0 38.0 38.0 38.0 38.0 10-14 37.468050000000005 38.0 38.0 38.0 37.6 38.0 15-19 37.460350000000005 38.0 38.0 38.0 37.6 38.0 20-24 37.39790000000001 38.0 38.0 38.0 37.2 38.0 25-29 37.31555 38.0 38.0 38.0 37.2 38.0 30-34 37.21585 38.0 38.0 38.0 37.0 38.0 35-39 37.1584 38.0 38.0 38.0 37.0 38.0 40-44 36.93345000000001 38.0 38.0 38.0 36.2 38.0 45-49 36.90175 38.0 38.0 38.0 36.0 38.0 50-54 36.90615 38.0 38.0 38.0 36.0 38.0 55-59 36.848650000000006 38.0 38.0 38.0 35.6 38.0 60-64 36.74825 38.0 38.0 38.0 35.2 38.0 65-69 36.661500000000004 38.0 38.0 38.0 35.0 38.0 70-74 36.402049999999996 38.0 38.0 38.0 34.2 38.0 75-79 36.08135 38.0 38.0 38.0 34.0 38.0 80-84 36.01535 38.0 38.0 38.0 34.0 38.0 85-89 35.91175 38.0 38.0 38.0 33.2 38.0 90-94 35.70375 38.0 37.8 38.0 33.0 38.0 95-99 35.5304 38.0 37.4 38.0 31.8 38.0 100-104 35.38635 38.0 37.0 38.0 31.2 38.0 105-109 35.25815 38.0 36.4 38.0 30.8 38.0 110-114 35.0084 38.0 36.0 38.0 29.4 38.0 115-119 34.80675 38.0 36.0 38.0 28.6 38.0 120-124 34.472 38.0 35.0 38.0 26.4 38.0 125-129 34.3887 38.0 35.2 38.0 26.2 38.0 130-134 34.08235 38.0 35.0 38.0 23.8 38.0 135-139 33.6804 38.0 34.4 38.0 22.2 38.0 140-144 33.223 38.0 34.0 38.0 16.2 38.0 145-149 32.5043 38.0 33.6 38.0 14.2 38.0 150-151 28.232125 35.5 24.0 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 2.0 7 4.0 8 1.0 9 6.0 10 3.0 11 2.0 12 3.0 13 3.0 14 4.0 15 9.0 16 10.0 17 6.0 18 20.0 19 33.0 20 9.0 21 8.0 22 8.0 23 10.0 24 14.0 25 14.0 26 7.0 27 26.0 28 26.0 29 46.0 30 34.0 31 44.0 32 66.0 33 105.0 34 157.0 35 293.0 36 772.0 37 2252.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.32447768893474 11.83905081248388 11.01367036368326 30.822801134898114 2 27.275 13.4 30.175 29.15 3 23.075000000000003 16.45 26.375 34.1 4 25.924999999999997 24.8 25.4 23.875 5 26.724999999999998 25.8 27.400000000000002 20.075000000000003 6 23.150000000000002 31.624999999999996 26.6 18.625 7 15.625 25.324999999999996 42.225 16.825000000000003 8 18.75 27.325 32.25 21.675 9 21.575 22.45 35.35 20.625 10-14 23.745 28.005000000000003 27.139999999999997 21.11 15-19 21.9 26.650000000000002 28.025 23.425 20-24 22.605 25.89 28.33 23.175 25-29 21.69 27.54 28.494999999999997 22.275 30-34 21.834999999999997 27.515 26.895000000000003 23.755000000000003 35-39 22.695 26.6 28.050000000000004 22.655 40-44 23.82 26.025 27.084999999999997 23.07 45-49 23.990000000000002 26.919999999999998 28.185 20.905 50-54 24.665 26.340000000000003 26.645000000000003 22.35 55-59 22.58 27.01 27.61 22.8 60-64 21.240000000000002 28.499999999999996 27.005000000000003 23.255 65-69 21.7 29.015 26.755000000000003 22.53 70-74 22.98 28.655 25.324999999999996 23.04 75-79 22.455 26.735 26.76 24.05 80-84 23.335 27.495000000000005 26.174999999999997 22.994999999999997 85-89 23.18 27.224999999999998 26.995 22.6 90-94 23.59 26.82 27.200000000000003 22.39 95-99 21.765 26.465 28.205000000000002 23.565 100-104 23.145 26.735 26.815 23.305 105-109 23.325000000000003 27.625 26.455000000000002 22.595000000000002 110-114 22.17 27.315 25.525 24.990000000000002 115-119 21.825 27.67 26.584999999999997 23.919999999999998 120-124 23.395 27.355 24.755 24.495 125-129 22.615 28.17 25.105 24.11 130-134 22.975 27.325 25.240000000000002 24.46 135-139 21.86 28.854999999999997 26.375 22.91 140-144 23.015 27.644999999999996 25.435000000000002 23.905 145-149 22.775000000000002 28.975 24.14 24.11 150-151 22.633487557834187 27.11016631236714 24.609228460672753 25.647117669125922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 1.5 3 1.0 4 2.5 5 2.5 6 2.5 7 3.5 8 3.0 9 1.5 10 0.5 11 1.0 12 1.0 13 1.0 14 0.5 15 0.0 16 2.5 17 4.0 18 1.5 19 0.0 20 0.5 21 2.0 22 4.0 23 4.5 24 3.0 25 2.5 26 3.5 27 4.5 28 6.0 29 22.0 30 32.5 31 52.5 32 64.0 33 74.5 34 100.5 35 108.0 36 131.0 37 182.0 38 198.5 39 163.0 40 158.5 41 137.5 42 98.0 43 86.0 44 92.5 45 115.0 46 137.0 47 144.5 48 143.0 49 147.5 50 142.5 51 137.5 52 153.5 53 166.0 54 141.0 55 110.0 56 94.0 57 77.5 58 68.5 59 62.0 60 50.0 61 40.0 62 35.0 63 43.5 64 41.0 65 28.5 66 20.0 67 17.0 68 18.5 69 17.0 70 14.5 71 10.0 72 15.0 73 17.5 74 11.5 75 8.0 76 5.5 77 3.0 78 0.5 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.425 #Duplication Level Percentage of deduplicated Percentage of total 1 85.15705902657922 61.675000000000004 2 8.111839834311356 11.75 3 2.7959958577839146 6.075 4 1.3117017604418364 3.8 5 0.9319986192613048 3.375 6 0.37970314118053156 1.6500000000000001 7 0.41422160856057993 2.1 8 0.17259233690024164 1.0 9 0.06903693476009665 0.44999999999999996 >10 0.6558508802209182 8.125 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 46 1.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC 45 1.125 TruSeq Adapter, Index 7 (97% over 35bp) GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 30 0.75 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 17 0.42500000000000004 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 16 0.4 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 16 0.4 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 15 0.375 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 14 0.35000000000000003 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 13 0.325 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 13 0.325 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 13 0.325 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 12 0.3 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 12 0.3 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 11 0.27499999999999997 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 11 0.27499999999999997 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 11 0.27499999999999997 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 10 0.25 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 10 0.25 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 10 0.25 No Hit GAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTT 9 0.22499999999999998 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 9 0.22499999999999998 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 8 0.2 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 8 0.2 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 8 0.2 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 8 0.2 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 8 0.2 No Hit GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT 7 0.17500000000000002 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 7 0.17500000000000002 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 7 0.17500000000000002 No Hit GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT 7 0.17500000000000002 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 7 0.17500000000000002 No Hit CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC 7 0.17500000000000002 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 7 0.17500000000000002 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 7 0.17500000000000002 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 7 0.17500000000000002 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 7 0.17500000000000002 No Hit CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA 7 0.17500000000000002 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 7 0.17500000000000002 No Hit CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT 6 0.15 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 6 0.15 No Hit CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA 6 0.15 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 6 0.15 No Hit GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG 6 0.15 No Hit GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG 6 0.15 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 6 0.15 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 6 0.15 No Hit ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 6 0.15 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6 0.15 No Hit GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT 6 0.15 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 5 0.125 No Hit CCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAG 5 0.125 No Hit GTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCAT 5 0.125 No Hit GCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTG 5 0.125 No Hit GATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAG 5 0.125 No Hit CACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCC 5 0.125 No Hit CAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTG 5 0.125 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 5 0.125 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 5 0.125 No Hit AACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTATATTTCGT 5 0.125 No Hit GCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAAT 5 0.125 No Hit CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT 5 0.125 No Hit AGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCG 5 0.125 No Hit CCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGG 5 0.125 No Hit CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT 5 0.125 No Hit TGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATAT 5 0.125 No Hit CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT 5 0.125 No Hit TGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAAC 5 0.125 No Hit GCAAAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATAT 5 0.125 No Hit GGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAG 5 0.125 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 5 0.125 No Hit ATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGAT 5 0.125 No Hit GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC 5 0.125 No Hit GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA 5 0.125 No Hit CCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGA 5 0.125 No Hit GCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTT 5 0.125 No Hit CCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0125 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.425 0.0 0.0 0.0 0.0 86-87 0.5875 0.0 0.0 0.0 0.0 88-89 0.6875 0.0 0.0 0.0 0.0 90-91 0.8 0.0 0.0 0.0 0.0 92-93 1.0750000000000002 0.0 0.0 0.0 0.0 94-95 1.1749999999999998 0.0 0.0 0.0 0.0 96-97 1.3 0.0 0.0 0.0 0.0 98-99 1.6875 0.0 0.0 0.0 0.0 100-101 1.95 0.0 0.0 0.0 0.0 102-103 2.1624999999999996 0.0 0.0 0.0 0.0 104-105 2.65 0.0 0.0 0.0 0.0 106-107 3.175 0.0 0.0 0.0 0.0 108-109 3.7 0.0 0.0 0.0 0.0 110-111 4.275 0.0 0.0 0.0 0.0 112-113 4.75 0.0 0.0 0.0 0.0 114-115 5.425 0.0 0.0 0.0 0.0 116-117 6.025 0.0 0.0 0.0 0.0 118-119 6.4125 0.0 0.0 0.0 0.0 120-121 7.012499999999999 0.0 0.0 0.0 0.0 122-123 7.7125 0.0 0.0 0.0 0.0 124-125 8.475000000000001 0.0 0.0 0.0 0.0 126-127 9.175 0.0 0.0 0.0 0.0 128-129 10.0875 0.0 0.0 0.0 0.0 130-131 10.8625 0.0 0.0 0.0 0.0 132-133 11.6125 0.0 0.0 0.0 0.0 134-135 12.3625 0.0 0.0 0.0 0.0 136-137 13.35 0.0 0.0 0.0 0.0 138-139 14.45 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTCCA 10 0.006832588 144.9875 4 CCAAAGT 10 0.006832588 144.9875 8 TGTCCAA 10 0.006832588 144.9875 5 CAATAGG 10 0.006832588 144.9875 6 CAAAGTT 10 0.006832588 144.9875 9 GTCCAAA 10 0.006832588 144.9875 6 TCCAAAG 10 0.006832588 144.9875 7 CTGCAAG 10 0.006832588 144.9875 145 AGTTCTT 10 0.006832588 144.9875 4 TGTGTCC 10 0.006832588 144.9875 3 CTTAGTT 40 5.8882564E-5 72.49375 7 TAGTTAC 40 5.8882564E-5 72.49375 9 ACTTAGT 40 5.8882564E-5 72.49375 6 CACTTAG 40 5.8882564E-5 72.49375 5 TCACTTA 40 5.8882564E-5 72.49375 4 GGGCTTT 30 0.0017979635 72.49375 145 GCCTCAC 45 9.900625E-5 65.25457 1 CTCACTT 45 1.0553847E-4 64.43889 3 CCTCACT 50 1.7777059E-4 57.995 2 TTAGTTA 85 6.8856207E-7 51.17206 8 >>END_MODULE SRR5578482 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578482_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8665 33.0 33.0 34.0 32.0 34.0 2 32.92025 33.0 33.0 34.0 32.0 34.0 3 32.89725 34.0 33.0 34.0 32.0 34.0 4 32.80325 34.0 33.0 34.0 32.0 34.0 5 32.87175 34.0 33.0 34.0 32.0 34.0 6 36.92725 38.0 38.0 38.0 36.0 38.0 7 36.951 38.0 38.0 38.0 37.0 38.0 8 36.91475 38.0 38.0 38.0 37.0 38.0 9 36.88 38.0 38.0 38.0 36.0 38.0 10-14 36.852 38.0 38.0 38.0 36.0 38.0 15-19 36.7819 38.0 38.0 38.0 36.2 38.0 20-24 36.78869999999999 38.0 38.0 38.0 36.2 38.0 25-29 36.8223 38.0 38.0 38.0 36.0 38.0 30-34 36.811899999999994 38.0 38.0 38.0 36.0 38.0 35-39 36.73715 38.0 38.0 38.0 36.0 38.0 40-44 36.759949999999996 38.0 38.0 38.0 36.0 38.0 45-49 36.6948 38.0 38.0 38.0 36.0 38.0 50-54 36.63590000000001 38.0 38.0 38.0 35.8 38.0 55-59 36.5893 38.0 38.0 38.0 35.4 38.0 60-64 36.61065 38.0 38.0 38.0 35.6 38.0 65-69 36.4246 38.0 38.0 38.0 35.2 38.0 70-74 36.119 38.0 38.0 38.0 34.2 38.0 75-79 36.08365 38.0 38.0 38.0 34.2 38.0 80-84 35.950900000000004 38.0 38.0 38.0 34.0 38.0 85-89 35.853449999999995 38.0 38.0 38.0 34.0 38.0 90-94 35.72515 38.0 38.0 38.0 33.4 38.0 95-99 35.618399999999994 38.0 38.0 38.0 33.2 38.0 100-104 35.426300000000005 38.0 38.0 38.0 32.2 38.0 105-109 35.29875 38.0 38.0 38.0 31.2 38.0 110-114 35.120149999999995 38.0 37.2 38.0 30.6 38.0 115-119 34.92405 38.0 36.4 38.0 29.2 38.0 120-124 34.37975 38.0 35.4 38.0 24.8 38.0 125-129 34.1894 38.0 35.2 38.0 24.0 38.0 130-134 33.84255 38.0 34.8 38.0 22.4 38.0 135-139 33.481700000000004 38.0 33.4 38.0 20.2 38.0 140-144 32.8224 38.0 33.0 38.0 13.6 38.0 145-149 31.70885 38.0 33.0 38.0 6.4 38.0 150-151 26.184625 33.0 17.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 10.0 4 3.0 5 4.0 6 4.0 7 0.0 8 3.0 9 4.0 10 5.0 11 4.0 12 3.0 13 7.0 14 11.0 15 12.0 16 16.0 17 32.0 18 6.0 19 12.0 20 6.0 21 12.0 22 10.0 23 15.0 24 13.0 25 20.0 26 18.0 27 30.0 28 29.0 29 34.0 30 38.0 31 52.0 32 69.0 33 95.0 34 126.0 35 272.0 36 625.0 37 2393.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.074999999999996 20.075000000000003 13.775 28.075 2 27.700000000000003 25.650000000000002 24.675 21.975 3 22.975 24.45 30.25 22.325 4 24.65 32.025 23.849999999999998 19.475 5 26.224999999999998 33.575 20.075000000000003 20.125 6 24.006001500375092 35.108777194298575 22.20555138784696 18.67966991747937 7 21.3 23.400000000000002 34.949999999999996 20.349999999999998 8 23.1 26.875 24.275 25.75 9 24.224999999999998 24.7 27.775 23.3 10-14 25.3113901255565 26.732029413235956 23.350507728477815 24.60607273272973 15-19 24.77734414089863 27.26408485940158 25.05253677574302 22.90603422395677 20-24 24.602221555088562 26.548584008806163 24.94746322425698 23.901731211848293 25-29 26.132212380523445 26.63764199569634 24.280638542761345 22.949507081018865 30-34 26.9973968762515 25.891069283139768 24.539447336804166 22.572086503804563 35-39 24.745983282446566 24.96120926973322 26.067370739276242 24.22543670854397 40-44 26.10958218663998 25.43907930948211 25.59919939954966 22.852139104328245 45-49 25.61048839071257 24.629703763010408 27.401921537229786 22.357886309047238 50-54 23.710153630586 24.33068107891708 28.689385978081365 23.269779312415555 55-59 24.00540459390482 25.876995446129207 28.374118000300257 21.74348195966572 60-64 22.72386005305571 25.461734821562644 28.16957805695981 23.644827068421844 65-69 23.051119010664397 27.807540179241975 26.79617483602864 22.345165974064987 70-74 21.957936905358036 26.97045568352529 27.035553329994993 24.036054081121684 75-79 21.347020530796193 28.452679018527792 26.27441161742614 23.925888833249875 80-84 22.56384576865298 27.290936404606907 26.790185277916873 23.355032548823235 85-89 21.8018018018018 28.53853853853854 27.112112112112115 22.54754754754755 90-94 23.028422738190553 28.527822257806246 26.871497197758202 21.572257806244995 95-99 22.77663780591562 28.807366998648714 26.77543666483159 21.640558530604075 100-104 23.229745283490967 28.79947955762398 25.421608367112047 22.549166791773008 105-109 23.158158158158155 30.36036036036036 25.085085085085083 21.396396396396398 110-114 22.237244003805518 28.66656652145611 24.986230033548644 24.109959441189723 115-119 23.160108140582757 31.05537198357865 24.121357765094622 21.663162110743965 120-124 23.697251839615557 29.644090704309956 24.978725534364518 21.679931921709965 125-129 23.434606336653484 30.041543620801843 24.57079933930627 21.9530507032384 130-134 24.41941941941942 28.348348348348345 25.560560560560557 21.67167167167167 135-139 24.163122341756317 29.497122842131603 24.813610207655742 21.52614460845634 140-144 25.78789394697349 27.283641820910454 25.947973986993496 20.98049024512256 145-149 25.485291174704823 28.6972183309986 25.22013207924755 20.59735841504903 150-151 27.072652244591723 26.384894335375762 25.534575465799676 21.007877954232836 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.5 6 1.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 0.0 19 0.5 20 1.5 21 1.5 22 1.0 23 1.5 24 1.0 25 0.5 26 1.5 27 4.0 28 6.5 29 11.5 30 18.5 31 31.5 32 36.5 33 33.5 34 56.0 35 85.5 36 100.0 37 141.0 38 192.5 39 190.5 40 260.5 41 217.0 42 84.0 43 90.0 44 102.0 45 109.5 46 128.0 47 131.5 48 136.5 49 139.5 50 118.0 51 109.5 52 142.0 53 169.5 54 179.5 55 175.0 56 132.0 57 94.5 58 90.5 59 80.0 60 57.0 61 46.5 62 36.5 63 37.0 64 33.5 65 25.5 66 19.5 67 18.0 68 19.5 69 14.5 70 15.0 71 15.0 72 10.5 73 12.0 74 10.5 75 5.5 76 3.5 77 3.0 78 2.0 79 1.5 80 0.5 81 0.0 82 0.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.025 7 0.0 8 0.0 9 0.0 10-14 0.045 15-19 0.06999999999999999 20-24 0.06999999999999999 25-29 0.08499999999999999 30-34 0.12 35-39 0.105 40-44 0.075 45-49 0.08 50-54 0.08499999999999999 55-59 0.08499999999999999 60-64 0.105 65-69 0.135 70-74 0.15 75-79 0.15 80-84 0.15 85-89 0.1 90-94 0.08 95-99 0.095 100-104 0.08499999999999999 105-109 0.1 110-114 0.145 115-119 0.13 120-124 0.11499999999999999 125-129 0.105 130-134 0.1 135-139 0.075 140-144 0.05 145-149 0.06 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.975 #Duplication Level Percentage of deduplicated Percentage of total 1 85.47260022066936 58.099999999999994 2 7.907318867230599 10.75 3 2.61125413755057 5.325 4 1.360794409709452 3.6999999999999997 5 0.5884516366311144 2.0 6 0.3677822728944465 1.5 7 0.33100404560500185 1.575 8 0.03677822728944465 0.2 9 0.1471129091577786 0.8999999999999999 >10 1.1769032732622289 15.950000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 50 1.25 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 45 1.125 Illumina Single End PCR Primer 1 (100% over 50bp) GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 38 0.95 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 35 0.8750000000000001 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 34 0.8500000000000001 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 32 0.8 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 30 0.75 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 28 0.7000000000000001 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 25 0.625 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 23 0.575 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 21 0.525 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 20 0.5 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 18 0.44999999999999996 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 18 0.44999999999999996 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 18 0.44999999999999996 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 17 0.42500000000000004 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 15 0.375 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 14 0.35000000000000003 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 14 0.35000000000000003 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 13 0.325 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 13 0.325 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 12 0.3 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 12 0.3 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 12 0.3 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 11 0.27499999999999997 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 10 0.25 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 10 0.25 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 10 0.25 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 10 0.25 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 10 0.25 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 10 0.25 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 10 0.25 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 9 0.22499999999999998 No Hit AATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTT 9 0.22499999999999998 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 9 0.22499999999999998 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 9 0.22499999999999998 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 8 0.2 No Hit GCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGT 7 0.17500000000000002 No Hit AAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTA 7 0.17500000000000002 No Hit GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG 7 0.17500000000000002 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 7 0.17500000000000002 No Hit CAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGC 7 0.17500000000000002 No Hit GGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTT 7 0.17500000000000002 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 7 0.17500000000000002 No Hit CTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATC 7 0.17500000000000002 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 7 0.17500000000000002 No Hit CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA 6 0.15 No Hit GCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT 6 0.15 No Hit CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA 6 0.15 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 6 0.15 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 6 0.15 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 6 0.15 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 6 0.15 No Hit CACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTAC 6 0.15 No Hit CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG 6 0.15 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 6 0.15 No Hit AAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT 5 0.125 No Hit CACGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGA 5 0.125 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 5 0.125 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 5 0.125 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 5 0.125 No Hit GGAGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCA 5 0.125 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 5 0.125 No Hit CCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTG 5 0.125 No Hit GCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAG 5 0.125 No Hit GGAAAAGTCACACTAGAGCGACACCAACATCGTTACGCTTACACACCGGA 5 0.125 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 5 0.125 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 5 0.125 No Hit CCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGAT 5 0.125 No Hit GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC 5 0.125 No Hit CGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTC 5 0.125 No Hit AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0125 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.2625 0.0 0.0 0.0 0.0 82-83 0.3625 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.6125 0.0 0.0 0.0 0.0 88-89 0.7124999999999999 0.0 0.0 0.0 0.0 90-91 0.8375 0.0 0.0 0.0 0.0 92-93 1.15 0.0 0.0 0.0 0.0 94-95 1.2875 0.0 0.0 0.0 0.0 96-97 1.4375 0.0 0.0 0.0 0.0 98-99 1.8 0.0 0.0 0.0 0.0 100-101 2.0999999999999996 0.0 0.0 0.0 0.0 102-103 2.3125 0.0 0.0 0.0 0.0 104-105 2.8375000000000004 0.0 0.0 0.0 0.0 106-107 3.4124999999999996 0.0 0.0 0.0 0.0 108-109 4.0 0.0 0.0 0.0 0.0 110-111 4.5375 0.0 0.0 0.0 0.0 112-113 5.0375 0.0 0.0 0.0 0.0 114-115 5.7375 0.0 0.0 0.0 0.0 116-117 6.325 0.0 0.0 0.0 0.0 118-119 6.7125 0.0 0.0 0.0 0.0 120-121 7.3125 0.0 0.0 0.0 0.0 122-123 8.0 0.0 0.0 0.0 0.0 124-125 8.725000000000001 0.0 0.0 0.0 0.0 126-127 9.4 0.0 0.0 0.0 0.0 128-129 10.4125 0.0 0.0 0.0 0.0 130-131 11.225000000000001 0.0 0.0 0.0 0.0 132-133 12.075 0.0 0.0 0.0 0.0 134-135 12.912500000000001 0.0 0.0 0.0 0.0 136-137 13.9 0.0 0.0 0.0 0.0 138-139 14.9875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTAAT 10 0.0070833815 143.25 1 TATATTC 25 8.6898333E-4 87.03797 145 GGATAGA 30 0.001886076 71.625 2 GGGATAG 30 0.001886076 71.625 1 GATAGAT 30 0.001886076 71.625 3 ATAGATA 35 0.0034757173 61.392857 4 TAGATAG 35 0.0034757173 61.392857 5 GCCCTAA 35 0.0035261218 20.723328 15-19 AAAGCCC 40 0.0076324623 18.132912 7 TGAAAGC 40 0.0076324623 18.132912 7 TTAGATA 40 0.0076324623 18.13291 85-89 >>END_MODULE Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663850 spots for SRR5578482.sra Written 663850 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra Read 663833 spots for SRR5578482.sra Written 663833 spots for SRR5578482.sra SRR ids: ['SRR5578482.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_imz7dze9 SRR5578482.sra spots: 13276677 blocks: [[1, 663833], [663834, 1327666], [1327667, 1991499], [1991500, 2655332], [2655333, 3319165], [3319166, 3982998], [3982999, 4646831], [4646832, 5310664], [5310665, 5974497], [5974498, 6638330], [6638331, 7302163], [7302164, 7965996], [7965997, 8629829], [8629830, 9293662], [9293663, 9957495], [9957496, 10621328], [10621329, 11285161], [11285162, 11948994], [11948995, 12612827], [12612828, 13276677]] SRR5578482 file size 4477329 SRR5578482 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578482 SRR5578482_1.fastq SRR5578482_2.fastq Input file: SRR5578482_1.fastq Paired file: SRR5578482_2.fastq trimmed: SRR5578482-trimmed-pair1.fastq, SRR5578482-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 20:22:15 2024 >> started Mon Dec 9 20:22:31 2024 >> done (16.344s) 13276677 read pairs processed; of these: 43444 ( 0.33%) short read pairs filtered out after trimming by size control 204821 ( 1.54%) empty read pairs filtered out after trimming by size control 13028412 (98.13%) read pairs available; of these: 7286741 (55.93%) trimmed read pairs available after processing 5741671 (44.07%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 7 0.00% 20 18 0.00% 21 21 0.00% 22 35 0.00% 23 23 0.00% 24 42 0.00% 25 32 0.00% 26 44 0.00% 27 45 0.00% 28 42 0.00% 29 45 0.00% 30 63 0.00% 31 108 0.00% 32 47 0.00% 33 46 0.00% 34 43 0.00% 35 70 0.00% 36 66 0.00% 37 68 0.00% 38 56 0.00% 39 68 0.00% 40 78 0.00% 41 80 0.00% 42 105 0.00% 43 122 0.00% 44 153 0.00% 45 202 0.00% 46 220 0.00% 47 274 0.00% 48 275 0.00% 49 311 0.00% 50 375 0.00% 51 388 0.00% 52 406 0.00% 53 410 0.00% 54 439 0.00% 55 490 0.00% 56 478 0.00% 57 557 0.00% 58 557 0.00% 59 578 0.00% 60 631 0.00% 61 730 0.01% 62 832 0.01% 63 1037 0.01% 64 1175 0.01% 65 1647 0.01% 66 2450 0.02% 67 3910 0.03% 68 6232 0.05% 69 12486 0.10% 70 11963 0.09% 71 5224 0.04% 72 3676 0.03% 73 3315 0.03% 74 3406 0.03% 75 3620 0.03% 76 3867 0.03% 77 4247 0.03% 78 4685 0.04% 79 5325 0.04% 80 5579 0.04% 81 6122 0.05% 82 7751 0.06% 83 8604 0.07% 84 10805 0.08% 85 13112 0.10% 86 14061 0.11% 87 15924 0.12% 88 16984 0.13% 89 17794 0.14% 90 17873 0.14% 91 17401 0.13% 92 18662 0.14% 93 20007 0.15% 94 20563 0.16% 95 21515 0.17% 96 22493 0.17% 97 22980 0.18% 98 24437 0.19% 99 25409 0.20% 100 27793 0.21% 101 29277 0.22% 102 29407 0.23% 103 31847 0.24% 104 33800 0.26% 105 35724 0.27% 106 38479 0.30% 107 38534 0.30% 108 40569 0.31% 109 39532 0.30% 110 40318 0.31% 111 42935 0.33% 112 44937 0.34% 113 50184 0.39% 114 54109 0.42% 115 56242 0.43% 116 56289 0.43% 117 54882 0.42% 118 54505 0.42% 119 54709 0.42% 120 56364 0.43% 121 57226 0.44% 122 60285 0.46% 123 62923 0.48% 124 65650 0.50% 125 66349 0.51% 126 68280 0.52% 127 68429 0.53% 128 65154 0.50% 129 70848 0.54% 130 70372 0.54% 131 69750 0.54% 132 73330 0.56% 133 76120 0.58% 134 78529 0.60% 135 78863 0.61% 136 78791 0.60% 137 79946 0.61% 138 85605 0.66% 139 88142 0.68% 140 91913 0.71% 141 92722 0.71% 142 107979 0.83% 143 110608 0.85% 144 119324 0.92% 145 137402 1.05% 146 165300 1.27% 147 202558 1.55% 148 291585 2.24% 149 559860 4.30% 150 2716432 20.85% 151 5741671 44.07% 13028412 reads passed initial QC criterion=sequence-density sequence-density=0.68 sequence-density-rank=1 fanout-score=21.62 fanout-score-rank=5 prefix-density=7.56 prefix-fanout=2.0 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=64.05 fanout-score-rank=1 prefix-density=0.63 prefix-fanout=1.3 sequence=TTTTTTTTCTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATTCCCGAGGCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCCCAGAGACGGGCGGAGGCTCTTAACCGTACGAGACAATCTCCA criterion=sequence-density sequence-density=1.22 sequence-density-rank=1 fanout-score=4.69 fanout-score-rank=12 prefix-density=5.68 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.16 sequence-density-rank=14 fanout-score=86.72 fanout-score-rank=1 prefix-density=13.64 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR5578482 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 20:24:41 Started mapping on | Dec 09 20:24:41 Finished on | Dec 09 20:55:26 Mapping speed, Million of reads per hour | 25.42 Number of input reads | 13028412 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 3577441 Uniquely mapped reads % | 27.46% Average mapped length | 283.26 Number of splices: Total | 2363470 Number of splices: Annotated (sjdb) | 2204347 Number of splices: GT/AG | 2335448 Number of splices: GC/AG | 22840 Number of splices: AT/AC | 1114 Number of splices: Non-canonical | 4068 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.01% Deletion average length | 1.38 Insertion rate per base | 0.01% Insertion average length | 1.29 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 81471 % of reads mapped to multiple loci | 0.63% Number of reads mapped to too many loci | 54141 % of reads mapped to too many loci | 0.42% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 70.25% % of reads unmapped: other | 1.25% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9376686 9376686 9376686 N_multimapping 81471 81471 81471 N_noFeature 121281 3429237 173144 N_ambiguous 117708 353 22291 UnstrandedReadsAssigned:3338452 PositiveStrandReadsAssigned:147851 NegativeStrandReadsAssigned:3382006 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=140 echo kmer=135 SRR5578482 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578482-trimmed-pair1.fastq SRR5578482-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 13,028,412 reads, 3,406,698 reads pseudoaligned [quant] estimated average fragment length: 199.211 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,003 rounds 52973 SRR5578482.ke.tsv 35125 SRR5578482.se.tsv 88098 total ==> SRR5578482.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 737.985 1.33583 0.536943 PNS24247 1044 845.789 0 0 PNS24249 1928 1729.79 23.4044 4.01354 PNS24246 1044 845.789 0 0 PNS24248 1044 845.789 0 0 PNS24244 1471 1272.79 63.2597 14.7433 PNS24243 293 118.877 0 0 KQK14069 1603 1404.79 163.709 34.5687 KQK14071 474 281.337 0 0 ==> SRR5578482.se.tsv <== BRADI_1g14170v3 175 BRADI_1g53295v3 0 BRADI_1g59795v3 20 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 39 BRADI_1g74790v3 271 BRADI_1g09890v3 0 BRADI_1g77505v3 47 BRADI_1g48960v3 0 SRR5578482 completed mapping pipeline successfully