Starting /dee2/code/volunteer_pipeline.sh SRR5578483 current disk space = 1521200795648 free memory = 1330318516 SRR5578483 SRAfilesize f22f7cd0669e342ca1c583da1f27a2cb SRR5578483.sra SRR5578483.sra file validated SRR5578483 is paired end SRR5578483 is conventional basespace SRR5578483 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578483_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2745 34.0 33.0 34.0 32.0 34.0 2 33.3175 34.0 34.0 34.0 32.0 34.0 3 33.38875 34.0 34.0 34.0 32.0 34.0 4 33.4275 34.0 34.0 34.0 33.0 34.0 5 33.471 34.0 34.0 34.0 33.0 34.0 6 37.18075 38.0 38.0 38.0 36.0 38.0 7 37.453 38.0 38.0 38.0 37.0 38.0 8 37.57325 38.0 38.0 38.0 38.0 38.0 9 37.5495 38.0 38.0 38.0 38.0 38.0 10-14 37.53875 38.0 38.0 38.0 38.0 38.0 15-19 37.56205 38.0 38.0 38.0 38.0 38.0 20-24 37.51585 38.0 38.0 38.0 38.0 38.0 25-29 37.43455 38.0 38.0 38.0 38.0 38.0 30-34 37.380849999999995 38.0 38.0 38.0 38.0 38.0 35-39 37.336400000000005 38.0 38.0 38.0 37.8 38.0 40-44 37.23564999999999 38.0 38.0 38.0 37.0 38.0 45-49 37.16355 38.0 38.0 38.0 37.0 38.0 50-54 37.1661 38.0 38.0 38.0 36.8 38.0 55-59 37.1671 38.0 38.0 38.0 36.8 38.0 60-64 37.05365 38.0 38.0 38.0 36.0 38.0 65-69 37.00355 38.0 38.0 38.0 36.0 38.0 70-74 36.88915000000001 38.0 38.0 38.0 35.8 38.0 75-79 36.7133 38.0 38.0 38.0 35.6 38.0 80-84 36.56875000000001 38.0 38.0 38.0 35.0 38.0 85-89 36.481449999999995 38.0 38.0 38.0 34.6 38.0 90-94 36.427 38.0 38.0 38.0 34.4 38.0 95-99 36.2261 38.0 38.0 38.0 34.0 38.0 100-104 36.124 38.0 38.0 38.0 34.0 38.0 105-109 36.00745 38.0 38.0 38.0 33.6 38.0 110-114 35.80145 38.0 38.0 38.0 33.0 38.0 115-119 35.6918 38.0 37.2 38.0 32.4 38.0 120-124 35.441700000000004 38.0 36.2 38.0 31.0 38.0 125-129 35.251 38.0 36.0 38.0 30.6 38.0 130-134 35.0492 38.0 35.6 38.0 29.6 38.0 135-139 34.58455 38.0 35.0 38.0 27.4 38.0 140-144 34.3055 38.0 35.0 38.0 26.0 38.0 145-149 33.4482 38.0 34.6 38.0 18.8 38.0 150-151 29.212625 35.5 27.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 2.0 8 2.0 9 0.0 10 0.0 11 3.0 12 7.0 13 1.0 14 4.0 15 4.0 16 1.0 17 6.0 18 10.0 19 12.0 20 4.0 21 6.0 22 7.0 23 5.0 24 6.0 25 14.0 26 13.0 27 28.0 28 25.0 29 36.0 30 31.0 31 45.0 32 53.0 33 94.0 34 123.0 35 223.0 36 622.0 37 2609.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.660598179453835 11.65149544863459 9.05071521456437 30.637191157347203 2 24.7 11.899999999999999 32.4 31.0 3 21.435717858929465 18.734367183591797 24.337168584292147 35.4927463731866 4 27.675 23.375 23.1 25.85 5 27.650000000000002 25.85 25.525 20.974999999999998 6 22.650000000000002 30.975 25.75 20.625 7 18.275 24.525 38.525 18.675 8 18.4 24.625 31.424999999999997 25.55 9 20.599999999999998 22.6 32.925 23.875 10-14 23.255 27.49 25.46 23.794999999999998 15-19 23.43 25.215 26.040000000000003 25.314999999999998 20-24 22.575 25.685000000000002 26.314999999999998 25.424999999999997 25-29 23.415 26.284999999999997 26.064999999999998 24.235 30-34 22.925 26.665 25.814999999999998 24.595 35-39 23.53 25.7 25.795 24.975 40-44 24.08 25.224999999999998 25.75 24.945 45-49 24.224999999999998 25.545 25.81 24.42 50-54 24.68 25.14 25.445 24.735 55-59 24.0 25.740000000000002 25.15 25.11 60-64 23.625 25.509999999999998 25.28 25.585 65-69 23.485 26.484999999999996 24.925 25.105 70-74 23.905 26.275 24.445 25.374999999999996 75-79 24.07 25.195 24.685000000000002 26.05 80-84 24.15 25.424999999999997 24.555 25.869999999999997 85-89 24.42 25.335 24.795 25.45 90-94 25.14 24.775 25.215 24.87 95-99 23.66 24.785 25.929999999999996 25.624999999999996 100-104 24.52 25.09 24.9 25.490000000000002 105-109 24.875 25.295 24.38 25.45 110-114 24.085 25.52 24.34 26.055 115-119 24.805 24.945 24.474999999999998 25.775 120-124 25.019999999999996 24.77 23.880000000000003 26.33 125-129 24.779999999999998 25.185000000000002 24.48 25.555 130-134 25.224999999999998 25.215 23.645 25.915 135-139 24.65 25.205 24.7 25.445 140-144 24.959999999999997 25.245 24.145 25.650000000000002 145-149 24.865000000000002 25.665 23.76 25.71 150-151 25.7375 24.587500000000002 23.25 26.424999999999997 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 2.0 2 2.0 3 2.5 4 2.0 5 2.5 6 1.5 7 1.0 8 2.0 9 1.0 10 0.5 11 0.5 12 0.5 13 0.5 14 0.5 15 0.5 16 0.5 17 1.0 18 1.0 19 1.5 20 1.0 21 1.0 22 2.0 23 1.0 24 0.0 25 1.5 26 1.5 27 1.5 28 4.0 29 13.0 30 22.5 31 30.5 32 34.5 33 36.0 34 49.0 35 65.5 36 86.5 37 117.5 38 128.5 39 124.5 40 129.0 41 123.0 42 120.0 43 124.5 44 120.0 45 117.0 46 135.0 47 152.5 48 149.5 49 150.5 50 139.0 51 133.0 52 141.5 53 133.5 54 123.5 55 106.5 56 96.0 57 102.0 58 95.5 59 81.0 60 88.5 61 92.5 62 80.0 63 68.5 64 61.5 65 59.5 66 51.5 67 47.0 68 47.0 69 36.5 70 27.0 71 24.0 72 21.5 73 18.0 74 13.0 75 12.0 76 12.0 77 9.5 78 5.0 79 3.0 80 2.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.875 2 0.0 3 0.05 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.14999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 93.91475042063936 83.72500000000001 2 4.038137969713965 7.199999999999999 3 0.89736399326977 2.4 4 0.33651149747616377 1.2 5 0.2243409983174425 1.0 6 0.1962983735277622 1.05 7 0.14021312394840157 0.8750000000000001 8 0.056085249579360626 0.4 9 0.08412787436904094 0.675 >10 0.11217049915872125 1.4749999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 20 0.5 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC 16 0.4 TruSeq Adapter, Index 22 (97% over 37bp) CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 13 0.325 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 10 0.25 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 9 0.22499999999999998 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 9 0.22499999999999998 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 9 0.22499999999999998 No Hit GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA 8 0.2 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 8 0.2 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 7 0.17500000000000002 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 7 0.17500000000000002 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 7 0.17500000000000002 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 7 0.17500000000000002 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 7 0.17500000000000002 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 6 0.15 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 6 0.15 No Hit CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA 6 0.15 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 6 0.15 No Hit GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA 6 0.15 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 6 0.15 No Hit GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG 6 0.15 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 5 0.125 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 5 0.125 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 5 0.125 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 5 0.125 No Hit ATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAG 5 0.125 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 5 0.125 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 5 0.125 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.23750000000000002 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.325 0.0 0.0 0.0 0.0 88-89 0.4375 0.0 0.0 0.0 0.0 90-91 0.55 0.0 0.0 0.0 0.0 92-93 0.7 0.0 0.0 0.0 0.0 94-95 0.8500000000000001 0.0 0.0 0.0 0.0 96-97 1.1 0.0 0.0 0.0 0.0 98-99 1.3 0.0 0.0 0.0 0.0 100-101 1.5875 0.0 0.0 0.0 0.0 102-103 1.9125 0.0 0.0 0.0 0.0 104-105 2.3 0.0 0.0 0.0 0.0 106-107 2.6875 0.0 0.0 0.0 0.0 108-109 2.95 0.0 0.0 0.0 0.0 110-111 3.3625 0.0 0.0 0.0 0.0 112-113 3.7125000000000004 0.0 0.0 0.0 0.0 114-115 4.199999999999999 0.0 0.0 0.0 0.0 116-117 4.6625 0.0 0.0 0.0 0.0 118-119 5.074999999999999 0.0 0.0 0.0 0.0 120-121 5.425000000000001 0.0 0.0 0.0 0.0 122-123 6.050000000000001 0.0 0.0 0.0 0.0 124-125 6.725 0.0 0.0 0.0 0.0 126-127 7.35 0.0 0.0 0.0 0.0 128-129 8.212499999999999 0.0 0.0 0.0 0.0 130-131 8.925 0.0 0.0 0.0 0.0 132-133 9.662500000000001 0.0 0.0 0.0 0.0 134-135 10.4125 0.0 0.0 0.0 0.0 136-137 11.2875 0.0 0.0 0.0 0.0 138-139 12.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR5578483 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578483_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.834 33.0 33.0 34.0 32.0 34.0 2 32.8935 34.0 33.0 34.0 32.0 34.0 3 32.89175 34.0 33.0 34.0 32.0 34.0 4 32.8675 34.0 33.0 34.0 32.0 34.0 5 32.8645 34.0 33.0 34.0 32.0 34.0 6 36.983 38.0 38.0 38.0 36.0 38.0 7 36.93075 38.0 38.0 38.0 37.0 38.0 8 36.9325 38.0 38.0 38.0 37.0 38.0 9 36.89925 38.0 38.0 38.0 37.0 38.0 10-14 36.8768 38.0 38.0 38.0 36.6 38.0 15-19 36.81205 38.0 38.0 38.0 36.2 38.0 20-24 36.81055 38.0 38.0 38.0 36.6 38.0 25-29 36.7838 38.0 38.0 38.0 36.6 38.0 30-34 36.779399999999995 38.0 38.0 38.0 36.2 38.0 35-39 36.754599999999996 38.0 38.0 38.0 36.2 38.0 40-44 36.75425 38.0 38.0 38.0 36.4 38.0 45-49 36.7098 38.0 38.0 38.0 36.2 38.0 50-54 36.691250000000004 38.0 38.0 38.0 36.0 38.0 55-59 36.6348 38.0 38.0 38.0 36.0 38.0 60-64 36.61655 38.0 38.0 38.0 36.0 38.0 65-69 36.486200000000004 38.0 38.0 38.0 35.4 38.0 70-74 36.319300000000005 38.0 38.0 38.0 34.8 38.0 75-79 36.2428 38.0 38.0 38.0 34.6 38.0 80-84 36.177749999999996 38.0 38.0 38.0 34.6 38.0 85-89 36.126549999999995 38.0 38.0 38.0 34.0 38.0 90-94 36.05315 38.0 38.0 38.0 34.0 38.0 95-99 35.81655 38.0 38.0 38.0 33.2 38.0 100-104 35.722950000000004 38.0 38.0 38.0 33.4 38.0 105-109 35.56825 38.0 38.0 38.0 33.0 38.0 110-114 35.3655 38.0 37.6 38.0 31.6 38.0 115-119 35.2101 38.0 36.8 38.0 31.2 38.0 120-124 34.90945 38.0 36.0 38.0 28.8 38.0 125-129 34.59005 38.0 35.8 38.0 26.8 38.0 130-134 34.13965 38.0 35.0 38.0 24.0 38.0 135-139 33.5609 38.0 33.4 38.0 21.8 38.0 140-144 33.12335 38.0 33.0 38.0 16.6 38.0 145-149 31.972999999999995 38.0 32.6 38.0 8.0 38.0 150-151 26.890375 34.5 17.0 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21.0 3 6.0 4 6.0 5 2.0 6 5.0 7 2.0 8 2.0 9 3.0 10 5.0 11 1.0 12 3.0 13 2.0 14 10.0 15 2.0 16 6.0 17 18.0 18 9.0 19 9.0 20 12.0 21 11.0 22 11.0 23 8.0 24 10.0 25 24.0 26 18.0 27 15.0 28 23.0 29 39.0 30 40.0 31 57.0 32 77.0 33 97.0 34 137.0 35 211.0 36 681.0 37 2417.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.65 18.5 10.75 27.1 2 28.23911955977989 24.23711855927964 23.58679339669835 23.936968484242122 3 25.819364523392547 24.568426319739807 25.619214410808105 23.992994746059544 4 27.177177177177175 29.72972972972973 19.694694694694697 23.3983983983984 5 27.745809357017766 33.45008756567426 19.11433575181386 19.68976732549412 6 23.400000000000002 32.875 20.424999999999997 23.3 7 23.35 20.25 32.550000000000004 23.849999999999998 8 23.925 23.825 23.75 28.499999999999996 9 24.349999999999998 23.799999999999997 25.4 26.450000000000003 10-14 26.40584350610366 25.310186111667 22.038222933760256 26.245747448469082 15-19 26.639631520977268 24.757184339641533 23.515570241313707 25.08761389806749 20-24 27.280464603985184 24.897366576549516 22.514268549113847 25.307900270351457 25-29 27.19807730823152 24.509313038253556 23.15742038854396 25.135189264970958 30-34 27.40610916374562 24.491737606409615 23.09464196294442 25.00751126690035 35-39 27.158021229721612 24.168836370919287 23.332665732024836 25.340476667334265 40-44 27.120764726490165 24.753515840048046 23.697512637005154 24.428206796456635 45-49 26.571257005604483 24.089271417133705 24.054243394715773 25.28522818254604 50-54 26.33133133133133 23.633633633633632 25.115115115115117 24.91991991991992 55-59 25.78078078078078 24.364364364364363 24.95995995995996 24.894894894894897 60-64 24.909873823352694 24.148808331664327 25.330462647706792 25.610855197276187 65-69 25.485679951932706 25.365511716402967 24.584418185459644 24.564390146204687 70-74 24.959943921490087 25.250350490686962 24.61446024434208 25.175245343480874 75-79 24.99749624436655 24.99248873309965 24.541812719078617 25.468202303455183 80-84 25.43815723585378 25.558337506259388 24.41662493740611 24.586880320480724 85-89 25.265265265265263 25.365365365365367 25.020020020020016 24.34934934934935 90-94 25.58546837469976 25.52542033626902 24.494595676541234 24.394515612489993 95-99 25.274120062083817 26.9213438141491 24.157612777249287 23.6469233465178 100-104 25.44689800210305 26.02774022332382 24.09493765960643 24.4304241149667 105-109 25.751051472060887 26.707390346485077 23.402763869417186 24.13879431203685 110-114 25.01752628943415 26.745117676514774 23.985978968452677 24.251377065598398 115-119 25.72601642299219 26.687362307230124 23.903464850791106 23.68315641898658 120-124 26.129193790686028 26.324486730095142 24.256384576865297 23.28993490235353 125-129 26.703720394572127 27.224475489459714 23.108507335636673 22.96329678033148 130-134 26.85087851028683 25.929819292186014 24.16278720528608 23.056514992241077 135-139 26.711369095276222 26.676341072858285 23.85408326661329 22.758206565252202 140-144 27.897553899254664 25.871642239007553 24.070831874343455 22.15997198739433 145-149 28.376282211658744 25.349011758819113 24.053039779834876 22.221666249687267 150-151 28.769692423105774 25.418854713678417 23.69342335583896 22.118029507376843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 0.5 3 1.0 4 1.0 5 0.0 6 0.5 7 0.5 8 0.0 9 1.0 10 1.5 11 0.5 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 1.0 18 0.5 19 0.5 20 0.5 21 1.0 22 2.5 23 1.5 24 0.5 25 0.5 26 0.5 27 2.5 28 5.5 29 8.0 30 11.5 31 16.5 32 19.5 33 22.5 34 30.5 35 46.5 36 67.0 37 95.5 38 116.0 39 113.5 40 137.0 41 121.0 42 94.0 43 109.0 44 110.0 45 120.5 46 120.5 47 110.0 48 126.5 49 138.5 50 115.5 51 116.0 52 131.0 53 132.5 54 149.5 55 151.5 56 131.0 57 115.0 58 113.5 59 119.5 60 109.5 61 95.5 62 83.0 63 82.5 64 76.0 65 66.0 66 67.0 67 56.0 68 52.0 69 62.0 70 52.5 71 39.0 72 33.0 73 27.0 74 22.5 75 12.5 76 8.5 77 7.5 78 4.5 79 2.5 80 1.5 81 1.5 82 2.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.075 4 0.1 5 0.075 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.06 15-19 0.13 20-24 0.13 25-29 0.13999999999999999 30-34 0.15 35-39 0.13999999999999999 40-44 0.095 45-49 0.08 50-54 0.1 55-59 0.1 60-64 0.13999999999999999 65-69 0.13999999999999999 70-74 0.13999999999999999 75-79 0.15 80-84 0.15 85-89 0.1 90-94 0.08 95-99 0.135 100-104 0.145 105-109 0.13999999999999999 110-114 0.15 115-119 0.13999999999999999 120-124 0.15 125-129 0.145 130-134 0.11499999999999999 135-139 0.08 140-144 0.045 145-149 0.075 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.125 #Duplication Level Percentage of deduplicated Percentage of total 1 94.14634146341463 82.025 2 3.472022955523673 6.05 3 1.1190817790530847 2.9250000000000003 4 0.22955523672883787 0.8 5 0.20086083213773312 0.8750000000000001 6 0.22955523672883787 1.2 7 0.05738880918220947 0.35000000000000003 8 0.11477761836441894 0.8 9 0.0860832137733142 0.675 >10 0.3443328550932568 4.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 24 0.6 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 19 0.475 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 16 0.4 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 16 0.4 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 14 0.35000000000000003 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 14 0.35000000000000003 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 14 0.35000000000000003 Illumina Single End PCR Primer 1 (100% over 50bp) CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 12 0.3 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 11 0.27499999999999997 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 11 0.27499999999999997 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 11 0.27499999999999997 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 10 0.25 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 9 0.22499999999999998 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 9 0.22499999999999998 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 9 0.22499999999999998 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 8 0.2 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 8 0.2 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 8 0.2 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 8 0.2 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 7 0.17500000000000002 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 7 0.17500000000000002 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 6 0.15 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 6 0.15 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 6 0.15 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 6 0.15 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 6 0.15 No Hit GGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTC 6 0.15 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 6 0.15 No Hit CGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGT 6 0.15 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 5 0.125 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 5 0.125 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 5 0.125 No Hit CGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGG 5 0.125 No Hit GGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAA 5 0.125 No Hit GAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCA 5 0.125 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.23750000000000002 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.325 0.0 0.0 0.0 0.0 88-89 0.42500000000000004 0.0 0.0 0.0 0.0 90-91 0.5375 0.0 0.0 0.0 0.0 92-93 0.7 0.0 0.0 0.0 0.0 94-95 0.8500000000000001 0.0 0.0 0.0 0.0 96-97 1.075 0.0 0.0 0.0 0.0 98-99 1.325 0.0 0.0 0.0 0.0 100-101 1.6375000000000002 0.0 0.0 0.0 0.0 102-103 2.025 0.0 0.0 0.0 0.0 104-105 2.3875 0.0 0.0 0.0 0.0 106-107 2.7375 0.0 0.0 0.0 0.0 108-109 3.0 0.0 0.0 0.0 0.0 110-111 3.3875 0.0 0.0 0.0 0.0 112-113 3.7 0.0 0.0 0.0 0.0 114-115 4.1375 0.0 0.0 0.0 0.0 116-117 4.550000000000001 0.0 0.0 0.0 0.0 118-119 5.0 0.0 0.0 0.0 0.0 120-121 5.3875 0.0 0.0 0.0 0.0 122-123 6.074999999999999 0.0 0.0 0.0 0.0 124-125 6.75 0.0 0.0 0.0 0.0 126-127 7.35 0.0 0.0 0.0 0.0 128-129 8.1875 0.0 0.0 0.0 0.0 130-131 8.95 0.0 0.0 0.0 0.0 132-133 9.7875 0.0 0.0 0.0 0.0 134-135 10.600000000000001 0.0 0.0 0.0 0.0 136-137 11.425 0.0 0.0 0.0 0.0 138-139 12.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGCGAC 10 0.006830828 145.0 9 CATGCGA 10 0.006830828 145.0 8 >>END_MODULE Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802194 spots for SRR5578483.sra Written 802194 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra Read 802188 spots for SRR5578483.sra Written 802188 spots for SRR5578483.sra SRR ids: ['SRR5578483.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_2ty73w3c SRR5578483.sra spots: 16043766 blocks: [[1, 802188], [802189, 1604376], [1604377, 2406564], [2406565, 3208752], [3208753, 4010940], [4010941, 4813128], [4813129, 5615316], [5615317, 6417504], [6417505, 7219692], [7219693, 8021880], [8021881, 8824068], [8824069, 9626256], [9626257, 10428444], [10428445, 11230632], [11230633, 12032820], [12032821, 12835008], [12835009, 13637196], [13637197, 14439384], [14439385, 15241572], [15241573, 16043766]] SRR5578483 file size 5415005 SRR5578483 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578483 SRR5578483_1.fastq SRR5578483_2.fastq Input file: SRR5578483_1.fastq Paired file: SRR5578483_2.fastq trimmed: SRR5578483-trimmed-pair1.fastq, SRR5578483-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 20:26:25 2024 >> started Mon Dec 9 20:27:51 2024 >> done (86.121s) 16043766 read pairs processed; of these: 51209 ( 0.32%) short read pairs filtered out after trimming by size control 117890 ( 0.73%) empty read pairs filtered out after trimming by size control 15874667 (98.95%) read pairs available; of these: 8469062 (53.35%) trimmed read pairs available after processing 7405605 (46.65%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 13 0.00% 19 12 0.00% 20 22 0.00% 21 29 0.00% 22 40 0.00% 23 35 0.00% 24 53 0.00% 25 51 0.00% 26 36 0.00% 27 41 0.00% 28 45 0.00% 29 40 0.00% 30 39 0.00% 31 65 0.00% 32 40 0.00% 33 39 0.00% 34 41 0.00% 35 55 0.00% 36 62 0.00% 37 69 0.00% 38 64 0.00% 39 73 0.00% 40 75 0.00% 41 63 0.00% 42 86 0.00% 43 115 0.00% 44 139 0.00% 45 168 0.00% 46 194 0.00% 47 175 0.00% 48 223 0.00% 49 297 0.00% 50 269 0.00% 51 329 0.00% 52 372 0.00% 53 365 0.00% 54 340 0.00% 55 373 0.00% 56 416 0.00% 57 499 0.00% 58 524 0.00% 59 545 0.00% 60 673 0.00% 61 724 0.00% 62 739 0.00% 63 855 0.01% 64 989 0.01% 65 1361 0.01% 66 1718 0.01% 67 2359 0.01% 68 3091 0.02% 69 9487 0.06% 70 10634 0.07% 71 4248 0.03% 72 3071 0.02% 73 2971 0.02% 74 3308 0.02% 75 3516 0.02% 76 3682 0.02% 77 4223 0.03% 78 4487 0.03% 79 5072 0.03% 80 5610 0.04% 81 6069 0.04% 82 6908 0.04% 83 8050 0.05% 84 10809 0.07% 85 13505 0.09% 86 15014 0.09% 87 16612 0.10% 88 17325 0.11% 89 17640 0.11% 90 18626 0.12% 91 17931 0.11% 92 18499 0.12% 93 19224 0.12% 94 19712 0.12% 95 20666 0.13% 96 21552 0.14% 97 22673 0.14% 98 23909 0.15% 99 25486 0.16% 100 26966 0.17% 101 27808 0.18% 102 29407 0.19% 103 31419 0.20% 104 32910 0.21% 105 35559 0.22% 106 37536 0.24% 107 38135 0.24% 108 40066 0.25% 109 40072 0.25% 110 41734 0.26% 111 43376 0.27% 112 45563 0.29% 113 48605 0.31% 114 52525 0.33% 115 55070 0.35% 116 55347 0.35% 117 56230 0.35% 118 56320 0.35% 119 57600 0.36% 120 61201 0.39% 121 59985 0.38% 122 61918 0.39% 123 63960 0.40% 124 65982 0.42% 125 67769 0.43% 126 70839 0.45% 127 71974 0.45% 128 72572 0.46% 129 76693 0.48% 130 76411 0.48% 131 77491 0.49% 132 81142 0.51% 133 83564 0.53% 134 85320 0.54% 135 86757 0.55% 136 89728 0.57% 137 91995 0.58% 138 98021 0.62% 139 102191 0.64% 140 105743 0.67% 141 110128 0.69% 142 121624 0.77% 143 128422 0.81% 144 139930 0.88% 145 159479 1.00% 146 188234 1.19% 147 238712 1.50% 148 341309 2.15% 149 663998 4.18% 150 3474163 21.88% 151 7405605 46.65% 15874667 reads passed initial QC criterion=sequence-density sequence-density=0.95 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=32 prefix-density=0.98 prefix-fanout=2.0 sequence=AACATGGAGAACATGGCGAGGCGGCCGTTCTTGATCTCCTTCACCTTGAGCTCAGCGAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=25.65 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=1.4 sequence=TTTTTTTTCCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATT criterion=sequence-density sequence-density=0.78 sequence-density-rank=1 fanout-score=3.14 fanout-score-rank=19 prefix-density=0.99 prefix-fanout=2.5 sequence=CTCGCCATGTTCTCCATGTTCGGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=56.21 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=1.4 sequence=CCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGGAAAACTGCGACTGTTCTTTAACCAAACATCCGTGCGATTCGTGCCACTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGGAGTTTCATGCTAATCAAAGAAGAGCTAATCCGACGTAAAGTTGCGGCGTTGATTACGCAGGATTGCGACCA SRR5578483 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 20:36:52 Started mapping on | Dec 09 20:36:52 Finished on | Dec 09 21:53:45 Mapping speed, Million of reads per hour | 12.39 Number of input reads | 15874667 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 10354955 Uniquely mapped reads % | 65.23% Average mapped length | 288.19 Number of splices: Total | 7677409 Number of splices: Annotated (sjdb) | 7258812 Number of splices: GT/AG | 7587613 Number of splices: GC/AG | 79048 Number of splices: AT/AC | 3998 Number of splices: Non-canonical | 6750 Mismatch rate per base, % | 0.10% Deletion rate per base | 0.00% Deletion average length | 1.36 Insertion rate per base | 0.00% Insertion average length | 1.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 106461 % of reads mapped to multiple loci | 0.67% Number of reads mapped to too many loci | 17108 % of reads mapped to too many loci | 0.11% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 33.59% % of reads unmapped: other | 0.41% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5433900 5433900 5433900 N_multimapping 106461 106461 106461 N_noFeature 178456 9989051 286296 N_ambiguous 298253 747 41414 UnstrandedReadsAssigned:9878246 PositiveStrandReadsAssigned:365157 NegativeStrandReadsAssigned:10027245 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR5578483 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578483-trimmed-pair1.fastq SRR5578483-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,874,667 reads, 10,103,834 reads pseudoaligned [quant] estimated average fragment length: 209.587 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,078 rounds 52973 SRR5578483.ke.tsv 35125 SRR5578483.se.tsv 88098 total ==> SRR5578483.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 727.509 3.47764 0.52311 PNS24247 1044 835.413 0 0 PNS24249 1928 1719.41 3.76239 0.239459 PNS24246 1044 835.413 0 0 PNS24248 1044 835.413 0 0 PNS24244 1471 1262.41 72.76 6.30723 PNS24243 293 110.391 0 0 KQK14069 1603 1394.41 137 10.7517 KQK14071 474 270.457 0 0 ==> SRR5578483.se.tsv <== BRADI_1g14170v3 135 BRADI_1g53295v3 12 BRADI_1g59795v3 85 BRADI_1g07683v3 0 BRADI_1g00485v3 9 BRADI_1g20270v3 221 BRADI_1g74790v3 362 BRADI_1g09890v3 5 BRADI_1g77505v3 159 BRADI_1g48960v3 0 SRR5578483 completed mapping pipeline successfully