Starting /dee2/code/volunteer_pipeline.sh SRR5578490 current disk space = 1521250279424 free memory = 1565400660 SRR5578490 SRAfilesize 7736417047ffe2197ff59056f58937f4 SRR5578490.sra SRR5578490.sra file validated SRR5578490 is paired end SRR5578490 is conventional basespace SRR5578490 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578490_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.89775 34.0 34.0 34.0 33.0 34.0 2 33.50575 34.0 34.0 34.0 33.0 34.0 3 33.54675 34.0 34.0 34.0 33.0 34.0 4 33.6125 34.0 34.0 34.0 33.0 34.0 5 33.63975 34.0 34.0 34.0 33.0 34.0 6 37.29425 38.0 38.0 38.0 36.0 38.0 7 37.5095 38.0 38.0 38.0 37.0 38.0 8 37.5905 38.0 38.0 38.0 38.0 38.0 9 37.66425 38.0 38.0 38.0 38.0 38.0 10-14 37.63375 38.0 38.0 38.0 38.0 38.0 15-19 37.640499999999996 38.0 38.0 38.0 38.0 38.0 20-24 37.609 38.0 38.0 38.0 38.0 38.0 25-29 37.515100000000004 38.0 38.0 38.0 38.0 38.0 30-34 37.456849999999996 38.0 38.0 38.0 38.0 38.0 35-39 37.420100000000005 38.0 38.0 38.0 38.0 38.0 40-44 37.18435000000001 38.0 38.0 38.0 37.0 38.0 45-49 37.24385 38.0 38.0 38.0 37.0 38.0 50-54 37.1662 38.0 38.0 38.0 37.0 38.0 55-59 37.144850000000005 38.0 38.0 38.0 37.0 38.0 60-64 37.11715 38.0 38.0 38.0 36.6 38.0 65-69 37.0495 38.0 38.0 38.0 36.0 38.0 70-74 36.83455 38.0 38.0 38.0 35.8 38.0 75-79 36.45355 38.0 38.0 38.0 35.0 38.0 80-84 36.320299999999996 38.0 38.0 38.0 34.8 38.0 85-89 36.1481 38.0 38.0 38.0 34.2 38.0 90-94 35.97135000000001 38.0 38.0 38.0 34.0 38.0 95-99 35.9345 38.0 38.0 38.0 34.0 38.0 100-104 35.7886 38.0 38.0 38.0 33.4 38.0 105-109 35.63175 38.0 37.8 38.0 33.0 38.0 110-114 35.35445 38.0 37.0 38.0 31.0 38.0 115-119 35.270250000000004 38.0 36.8 38.0 31.0 38.0 120-124 35.154399999999995 38.0 36.2 38.0 30.6 38.0 125-129 34.9967 38.0 36.0 38.0 30.2 38.0 130-134 34.622049999999994 38.0 35.6 38.0 27.8 38.0 135-139 34.321549999999995 38.0 35.0 38.0 26.0 38.0 140-144 33.832499999999996 38.0 35.0 38.0 22.8 38.0 145-149 33.1604 38.0 34.0 38.0 17.0 38.0 150-151 29.370875 36.0 27.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 0.0 7 7.0 8 4.0 9 1.0 10 3.0 11 0.0 12 1.0 13 0.0 14 4.0 15 4.0 16 3.0 17 8.0 18 20.0 19 35.0 20 9.0 21 7.0 22 7.0 23 11.0 24 10.0 25 16.0 26 19.0 27 15.0 28 16.0 29 29.0 30 32.0 31 31.0 32 51.0 33 61.0 34 99.0 35 230.0 36 651.0 37 2614.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.15676229508197 11.654713114754097 10.399590163934427 30.78893442622951 2 28.749999999999996 12.2 29.099999999999998 29.95 3 24.193144858643983 16.112084063047284 26.069552164123095 33.62521891418564 4 25.174999999999997 23.25 25.424999999999997 26.150000000000002 5 26.450000000000003 26.575 27.725 19.25 6 24.075 30.049999999999997 26.700000000000003 19.175 7 15.925 24.525 42.225 17.325 8 20.375 24.95 31.7 22.975 9 21.05 23.1 35.075 20.775 10-14 23.728559283892583 26.979046857028553 26.979046857028553 22.313347002050307 15-19 22.814999999999998 26.05 27.785 23.35 20-24 23.49 24.685000000000002 28.555000000000003 23.27 25-29 21.4 26.424999999999997 29.285 22.89 30-34 20.979999999999997 27.215 27.425 24.38 35-39 22.715 26.009999999999998 27.35 23.925 40-44 24.705 25.205 26.965 23.125 45-49 23.69 26.38 28.18 21.75 50-54 24.654999999999998 25.424999999999997 26.790000000000003 23.13 55-59 22.895 25.945 28.060000000000002 23.1 60-64 21.55 27.845 26.700000000000003 23.905 65-69 21.83 28.415000000000003 26.375 23.380000000000003 70-74 22.86 28.395 25.569999999999997 23.175 75-79 22.465 26.474999999999998 26.334999999999997 24.725 80-84 23.205000000000002 27.405 26.095000000000002 23.294999999999998 85-89 23.84 26.040000000000003 26.979999999999997 23.14 90-94 23.525 25.855 27.07 23.549999999999997 95-99 21.44 25.86 28.505000000000003 24.195 100-104 23.505000000000003 26.275 26.46 23.76 105-109 23.369999999999997 26.895000000000003 26.75 22.985 110-114 22.14 26.19 26.419999999999998 25.25 115-119 22.505 27.224999999999998 25.81 24.46 120-124 23.369999999999997 27.175 24.45 25.005 125-129 22.765 27.155 24.64 25.44 130-134 23.46 26.88 25.740000000000002 23.919999999999998 135-139 21.93 28.634999999999998 26.58 22.855 140-144 22.869999999999997 28.410000000000004 24.855 23.865 145-149 22.68 29.595 22.95 24.775 150-151 22.8 26.5875 24.9125 25.7 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 1.5 3 1.0 4 1.5 5 1.5 6 1.5 7 1.0 8 0.0 9 0.5 10 1.0 11 1.0 12 0.5 13 0.0 14 0.0 15 1.5 16 2.5 17 1.0 18 0.5 19 2.0 20 2.5 21 2.0 22 1.0 23 0.5 24 2.5 25 2.5 26 0.5 27 2.0 28 6.5 29 20.5 30 38.5 31 53.0 32 62.5 33 72.0 34 80.5 35 92.5 36 126.5 37 168.5 38 172.0 39 132.5 40 140.0 41 138.5 42 94.5 43 82.5 44 83.0 45 92.0 46 112.0 47 133.5 48 144.0 49 138.0 50 148.0 51 170.5 52 171.5 53 172.0 54 165.0 55 145.5 56 138.0 57 118.0 58 108.0 59 97.5 60 66.5 61 52.0 62 38.5 63 40.5 64 41.0 65 20.5 66 12.0 67 12.0 68 10.5 69 10.5 70 8.0 71 7.0 72 10.5 73 8.0 74 2.0 75 0.5 76 2.5 77 2.5 78 2.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.4 2 0.0 3 0.075 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.375 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38939670932358 55.65 2 10.383912248628885 14.2 3 3.9122486288848264 8.025 4 1.5356489945155392 4.2 5 0.8043875685557587 2.75 6 0.5850091407678245 2.4 7 0.29250457038391225 1.4000000000000001 8 0.18281535648994515 1.0 9 0.21937842778793418 1.35 >10 0.6215722120658135 6.225 >50 0.07312614259597806 2.8000000000000003 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 58 1.4500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC 54 1.35 TruSeq Adapter, Index 2 (97% over 36bp) GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 23 0.575 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 20 0.5 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 19 0.475 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 16 0.4 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 16 0.4 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 15 0.375 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 15 0.375 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 15 0.375 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 15 0.375 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 14 0.35000000000000003 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 14 0.35000000000000003 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 14 0.35000000000000003 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 11 0.27499999999999997 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 11 0.27499999999999997 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 11 0.27499999999999997 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 10 0.25 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 10 0.25 No Hit GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA 9 0.22499999999999998 No Hit CTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGC 9 0.22499999999999998 No Hit CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT 9 0.22499999999999998 No Hit TGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATAT 9 0.22499999999999998 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 9 0.22499999999999998 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 9 0.22499999999999998 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 8 0.2 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 8 0.2 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 8 0.2 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 8 0.2 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 8 0.2 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 7 0.17500000000000002 No Hit GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA 7 0.17500000000000002 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 7 0.17500000000000002 No Hit GGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAAT 7 0.17500000000000002 No Hit CAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTG 7 0.17500000000000002 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 7 0.17500000000000002 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 7 0.17500000000000002 No Hit CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA 7 0.17500000000000002 No Hit CAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCA 6 0.15 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 6 0.15 No Hit ACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAAT 6 0.15 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 6 0.15 No Hit TGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATG 6 0.15 No Hit GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT 6 0.15 No Hit GCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTA 6 0.15 No Hit GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA 6 0.15 No Hit CAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTAT 6 0.15 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 6 0.15 No Hit GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA 6 0.15 No Hit GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG 6 0.15 No Hit GGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG 6 0.15 No Hit GCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAA 6 0.15 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 6 0.15 No Hit GGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGC 6 0.15 No Hit CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG 5 0.125 No Hit CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA 5 0.125 No Hit GCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTG 5 0.125 No Hit GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT 5 0.125 No Hit GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT 5 0.125 No Hit CCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAA 5 0.125 No Hit ATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATA 5 0.125 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 5 0.125 No Hit GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT 5 0.125 No Hit GTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCT 5 0.125 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 5 0.125 No Hit CATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAA 5 0.125 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5 0.125 No Hit CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA 5 0.125 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5 0.125 No Hit GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT 5 0.125 No Hit TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT 5 0.125 No Hit GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC 5 0.125 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 5 0.125 No Hit GCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATG 5 0.125 No Hit CAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATA 5 0.125 No Hit GCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.2375 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.32499999999999996 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.55 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.7875 0.0 0.0 0.0 0.0 94-95 0.925 0.0 0.0 0.0 0.0 96-97 1.0875 0.0 0.0 0.0 0.0 98-99 1.2000000000000002 0.0 0.0 0.0 0.0 100-101 1.4125 0.0 0.0 0.0 0.0 102-103 1.675 0.0 0.0 0.0 0.0 104-105 1.925 0.0 0.0 0.0 0.0 106-107 2.2625 0.0 0.0 0.0 0.0 108-109 2.675 0.0 0.0 0.0 0.0 110-111 3.05 0.0 0.0 0.0 0.0 112-113 3.5375 0.0 0.0 0.0 0.0 114-115 4.050000000000001 0.0 0.0 0.0 0.0 116-117 4.6 0.0 0.0 0.0 0.0 118-119 5.1375 0.0 0.0 0.0 0.0 120-121 5.699999999999999 0.0 0.0 0.0 0.0 122-123 6.2875 0.0 0.0 0.0 0.0 124-125 6.9375 0.0 0.0 0.0 0.0 126-127 7.4625 0.0 0.0 0.0 0.0 128-129 7.9375 0.0 0.0 0.0 0.0 130-131 8.775 0.0 0.0 0.0 0.0 132-133 9.4875 0.0 0.0 0.0 0.0 134-135 10.1 0.0 0.0 0.0 0.0 136-137 10.725000000000001 0.0 0.0 0.0 0.0 138-139 11.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACACG 40 0.0076550315 18.125 140-144 >>END_MODULE SRR5578490 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578490_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.96975 33.0 33.0 34.0 32.0 34.0 2 33.0135 34.0 33.0 34.0 32.0 34.0 3 33.047 34.0 33.0 34.0 33.0 34.0 4 32.98575 34.0 33.0 34.0 32.0 34.0 5 33.03325 34.0 33.0 34.0 33.0 34.0 6 37.121 38.0 38.0 38.0 37.0 38.0 7 37.095 38.0 38.0 38.0 37.0 38.0 8 37.04725 38.0 38.0 38.0 37.0 38.0 9 37.08925 38.0 38.0 38.0 37.0 38.0 10-14 37.03825 38.0 38.0 38.0 37.0 38.0 15-19 37.000600000000006 38.0 38.0 38.0 37.0 38.0 20-24 36.99865 38.0 38.0 38.0 37.0 38.0 25-29 36.98755 38.0 38.0 38.0 37.0 38.0 30-34 36.982549999999996 38.0 38.0 38.0 37.0 38.0 35-39 36.9471 38.0 38.0 38.0 37.0 38.0 40-44 36.96145 38.0 38.0 38.0 37.0 38.0 45-49 36.953649999999996 38.0 38.0 38.0 37.0 38.0 50-54 36.898849999999996 38.0 38.0 38.0 37.0 38.0 55-59 36.866749999999996 38.0 38.0 38.0 37.0 38.0 60-64 36.8538 38.0 38.0 38.0 37.0 38.0 65-69 36.63025 38.0 38.0 38.0 36.4 38.0 70-74 36.18575 38.0 38.0 38.0 35.8 38.0 75-79 36.2435 38.0 38.0 38.0 36.0 38.0 80-84 36.179500000000004 38.0 38.0 38.0 35.8 38.0 85-89 36.070299999999996 38.0 38.0 38.0 35.0 38.0 90-94 36.01345 38.0 38.0 38.0 34.8 38.0 95-99 35.937850000000005 38.0 38.0 38.0 34.4 38.0 100-104 35.743 38.0 38.0 38.0 34.0 38.0 105-109 35.6278 38.0 38.0 38.0 33.8 38.0 110-114 35.5011 38.0 38.0 38.0 33.0 38.0 115-119 35.39920000000001 38.0 38.0 38.0 32.8 38.0 120-124 35.154250000000005 38.0 37.6 38.0 31.6 38.0 125-129 34.888850000000005 38.0 36.6 38.0 29.6 38.0 130-134 34.578700000000005 38.0 36.0 38.0 28.0 38.0 135-139 34.1072 38.0 35.8 38.0 25.0 38.0 140-144 33.54685 38.0 34.0 38.0 21.6 38.0 145-149 32.694900000000004 38.0 33.0 38.0 12.8 38.0 150-151 27.51775 34.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16.0 3 8.0 4 3.0 5 4.0 6 3.0 7 3.0 8 6.0 9 2.0 10 2.0 11 3.0 12 6.0 13 2.0 14 6.0 15 4.0 16 10.0 17 45.0 18 9.0 19 4.0 20 14.0 21 11.0 22 11.0 23 13.0 24 4.0 25 11.0 26 15.0 27 17.0 28 18.0 29 23.0 30 24.0 31 48.0 32 40.0 33 51.0 34 111.0 35 176.0 36 506.0 37 2771.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.725 20.275000000000002 13.8 27.200000000000003 2 27.125 26.6 24.45 21.825 3 24.224999999999998 25.25 28.499999999999996 22.025 4 24.4 32.0 22.625 20.974999999999998 5 28.575 32.525 20.4 18.5 6 26.075 32.725 21.825 19.375 7 21.75 22.525000000000002 34.25 21.475 8 21.95 26.650000000000002 26.150000000000002 25.25 9 24.275 24.099999999999998 27.500000000000004 24.125 10-14 26.125 26.615 22.745 24.515 15-19 25.216304076019004 26.65166291572893 24.82620655163791 23.305826456614152 20-24 25.697569756975696 27.16771677167717 24.147414741474147 22.987298729872986 25-29 26.03082465972778 26.62630104083267 24.394515612489993 22.94835868694956 30-34 26.832323029908324 25.92555483192225 24.658083262361604 22.584038875807824 35-39 25.68223924690802 24.48550398077212 25.847478844324268 23.984777927995594 40-44 26.488541979385573 25.60792554788352 24.892424697288103 23.01110777544281 45-49 25.593033730357323 24.657191472325092 26.078470623561206 23.67130417375638 50-54 24.545954870665934 24.69605243408215 27.512883374193226 23.24510932105869 55-59 23.6876377479463 25.841514726507715 28.230815467842113 22.240032057703868 60-64 22.836104988980164 26.101983570426768 27.39430975756361 23.667601683029453 65-69 22.972769670528056 27.62649816960032 27.125018805476152 22.275713354395464 70-74 22.7903945455457 27.467789642552766 26.354840326866196 23.386975485035343 75-79 21.851981760785687 27.849877236057523 26.12617126822669 24.171969734930098 80-84 22.864875701684042 28.327987169206093 25.240577385725743 23.566559743384122 85-89 22.677472609935464 28.21051578368102 26.104357396568112 23.0076542098154 90-94 23.5703207084605 28.343423225096316 25.706709361084705 22.379546705358482 95-99 23.517638228671505 29.407055291468602 25.72929697272955 21.346009507130347 100-104 23.443682075424448 28.972805128461964 24.82596283868383 22.75754995742976 105-109 23.74987473694759 29.922837959715405 24.531516183986373 21.795771119350636 110-114 22.306380417335472 29.414125200642054 24.729133226324237 23.550361155698234 115-119 23.64949591212319 30.27035160756383 23.995586096203038 22.084566384109948 120-124 23.77776663490949 29.890187033044175 24.28420999849571 22.04783633355062 125-129 23.925580462363975 30.23920565668723 23.559500526553332 22.275713354395464 130-134 24.217652958876627 28.36008024072217 25.25075225677031 22.171514543630895 135-139 24.058304948908035 29.16750150270487 24.559206571829293 22.214986976557803 140-144 25.918878317476214 27.911867801702556 25.408112168252377 20.761141712568854 145-149 26.170329945426325 27.77249286536825 25.07384969709107 20.983327492114352 150-151 26.02225834688008 26.559959984994375 24.946855070651495 22.470926597474055 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 0.5 18 1.0 19 2.0 20 1.0 21 2.0 22 2.0 23 1.0 24 1.5 25 0.5 26 1.0 27 2.5 28 5.0 29 6.5 30 15.0 31 29.0 32 32.0 33 39.0 34 62.5 35 81.5 36 104.0 37 144.5 38 180.5 39 170.0 40 225.0 41 189.0 42 79.5 43 90.0 44 91.5 45 84.0 46 90.0 47 106.0 48 125.5 49 132.0 50 130.5 51 120.0 52 153.5 53 210.0 54 213.0 55 204.5 56 183.5 57 131.0 58 101.0 59 91.0 60 63.0 61 48.5 62 47.5 63 38.0 64 26.0 65 21.5 66 13.5 67 12.0 68 17.0 69 16.5 70 13.0 71 8.5 72 7.0 73 4.5 74 3.0 75 6.0 76 5.0 77 4.5 78 5.0 79 2.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.025 20-24 0.01 25-29 0.08 30-34 0.19499999999999998 35-39 0.145 40-44 0.06999999999999999 45-49 0.09 50-54 0.065 55-59 0.18 60-64 0.18 65-69 0.295 70-74 0.265 75-79 0.215 80-84 0.24 85-89 0.055 90-94 0.065 95-99 0.075 100-104 0.165 105-109 0.21 110-114 0.32 115-119 0.315 120-124 0.28500000000000003 125-129 0.295 130-134 0.3 135-139 0.18 140-144 0.15 145-149 0.135 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.9 #Duplication Level Percentage of deduplicated Percentage of total 1 81.77966101694916 53.075 2 10.208012326656394 13.25 3 3.1587057010785826 6.15 4 1.50231124807396 3.9 5 1.0400616332819723 3.375 6 0.539291217257319 2.1 7 0.2696456086286595 1.225 8 0.30816640986132515 1.6 9 0.15408320493066258 0.8999999999999999 >10 1.0015408320493067 13.075000000000001 >50 0.038520801232665644 1.35 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 54 1.35 Illumina Single End PCR Primer 1 (100% over 50bp) CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 49 1.225 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 33 0.8250000000000001 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 30 0.75 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 28 0.7000000000000001 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 28 0.7000000000000001 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 26 0.65 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 26 0.65 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 25 0.625 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 25 0.625 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 22 0.5499999999999999 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 20 0.5 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 20 0.5 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 20 0.5 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 19 0.475 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 17 0.42500000000000004 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 16 0.4 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 16 0.4 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 16 0.4 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 13 0.325 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 12 0.3 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 11 0.27499999999999997 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 11 0.27499999999999997 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 10 0.25 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 10 0.25 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 10 0.25 No Hit GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC 10 0.25 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 9 0.22499999999999998 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 9 0.22499999999999998 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 9 0.22499999999999998 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 9 0.22499999999999998 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 8 0.2 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 8 0.2 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 8 0.2 No Hit GTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAA 8 0.2 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 8 0.2 No Hit CACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGG 8 0.2 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 8 0.2 No Hit GGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAA 8 0.2 No Hit GGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACT 7 0.17500000000000002 No Hit ACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC 7 0.17500000000000002 No Hit AAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTA 7 0.17500000000000002 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 7 0.17500000000000002 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 7 0.17500000000000002 No Hit GACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCA 7 0.17500000000000002 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 7 0.17500000000000002 No Hit TGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACA 6 0.15 No Hit AAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT 6 0.15 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 6 0.15 No Hit GGAGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCA 6 0.15 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 6 0.15 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 6 0.15 No Hit AAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGG 6 0.15 No Hit GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT 6 0.15 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 6 0.15 No Hit CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA 6 0.15 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 6 0.15 No Hit CTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATC 6 0.15 No Hit GCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACT 6 0.15 No Hit AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC 6 0.15 No Hit CAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCC 5 0.125 No Hit CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG 5 0.125 No Hit GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC 5 0.125 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 5 0.125 No Hit CAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCG 5 0.125 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 5 0.125 No Hit CCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCG 5 0.125 No Hit CATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAAT 5 0.125 No Hit AATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTT 5 0.125 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 5 0.125 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 5 0.125 No Hit GCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTG 5 0.125 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 5 0.125 No Hit CTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGATC 5 0.125 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 5 0.125 No Hit TAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAA 5 0.125 No Hit GTCGGCCAGTGAGACGATGGGGGATAAGCTTCATCGTCGAGAGGGAAACA 5 0.125 No Hit AAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA 5 0.125 No Hit AGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATCAGGC 5 0.125 No Hit GCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTAT 5 0.125 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 5 0.125 No Hit GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC 5 0.125 No Hit TTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGG 5 0.125 No Hit GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGGGTCGGAA 5 0.125 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 5 0.125 No Hit CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG 5 0.125 No Hit TCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.2375 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.32499999999999996 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.55 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.6875 0.0 0.0 0.0 0.0 92-93 0.7875 0.0 0.0 0.0 0.0 94-95 0.9 0.0 0.0 0.0 0.0 96-97 1.075 0.0 0.0 0.0 0.0 98-99 1.2000000000000002 0.0 0.0 0.0 0.0 100-101 1.3875000000000002 0.0 0.0 0.0 0.0 102-103 1.6125 0.0 0.0 0.0 0.0 104-105 1.875 0.0 0.0 0.0 0.0 106-107 2.25 0.0 0.0 0.0 0.0 108-109 2.675 0.0 0.0 0.0 0.0 110-111 3.05 0.0 0.0 0.0 0.0 112-113 3.55 0.0 0.0 0.0 0.0 114-115 4.0625 0.0 0.0 0.0 0.0 116-117 4.6 0.0 0.0 0.0 0.0 118-119 5.1375 0.0 0.0 0.0 0.0 120-121 5.6375 0.0 0.0 0.0 0.0 122-123 6.1625 0.0 0.0 0.0 0.0 124-125 6.85 0.0 0.0 0.0 0.0 126-127 7.4 0.0 0.0 0.0 0.0 128-129 7.9 0.0 0.0 0.0 0.0 130-131 8.775 0.0 0.0 0.0 0.0 132-133 9.625 0.0 0.0 0.0 0.0 134-135 10.275 0.0 0.0 0.0 0.0 136-137 10.9125 0.0 0.0 0.0 0.0 138-139 11.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGGG 10 0.006830828 145.0 6 ATATTCC 10 0.006830828 145.0 145 GCGTCGT 40 0.0076550315 18.125 140-144 >>END_MODULE Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639490 spots for SRR5578490.sra Written 639490 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra Read 639474 spots for SRR5578490.sra Written 639474 spots for SRR5578490.sra SRR ids: ['SRR5578490.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0oiscpu9 SRR5578490.sra spots: 12789496 blocks: [[1, 639474], [639475, 1278948], [1278949, 1918422], [1918423, 2557896], [2557897, 3197370], [3197371, 3836844], [3836845, 4476318], [4476319, 5115792], [5115793, 5755266], [5755267, 6394740], [6394741, 7034214], [7034215, 7673688], [7673689, 8313162], [8313163, 8952636], [8952637, 9592110], [9592111, 10231584], [10231585, 10871058], [10871059, 11510532], [11510533, 12150006], [12150007, 12789496]] SRR5578490 file size 4312240 SRR5578490 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578490 SRR5578490_1.fastq SRR5578490_2.fastq Input file: SRR5578490_1.fastq Paired file: SRR5578490_2.fastq trimmed: SRR5578490-trimmed-pair1.fastq, SRR5578490-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 20:35:24 2024 >> started Mon Dec 9 20:35:41 2024 >> done (16.819s) 12789496 read pairs processed; of these: 28724 ( 0.22%) short read pairs filtered out after trimming by size control 196172 ( 1.53%) empty read pairs filtered out after trimming by size control 12564600 (98.24%) read pairs available; of these: 6479007 (51.57%) trimmed read pairs available after processing 6085593 (48.43%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 13 0.00% 20 3 0.00% 21 20 0.00% 22 33 0.00% 23 23 0.00% 24 39 0.00% 25 29 0.00% 26 27 0.00% 27 30 0.00% 28 25 0.00% 29 26 0.00% 30 36 0.00% 31 28 0.00% 32 24 0.00% 33 30 0.00% 34 26 0.00% 35 30 0.00% 36 22 0.00% 37 41 0.00% 38 37 0.00% 39 48 0.00% 40 33 0.00% 41 51 0.00% 42 43 0.00% 43 58 0.00% 44 95 0.00% 45 109 0.00% 46 152 0.00% 47 182 0.00% 48 167 0.00% 49 184 0.00% 50 245 0.00% 51 251 0.00% 52 300 0.00% 53 267 0.00% 54 297 0.00% 55 307 0.00% 56 350 0.00% 57 365 0.00% 58 447 0.00% 59 389 0.00% 60 413 0.00% 61 555 0.00% 62 612 0.00% 63 694 0.01% 64 803 0.01% 65 1110 0.01% 66 1957 0.02% 67 3603 0.03% 68 3705 0.03% 69 7842 0.06% 70 9617 0.08% 71 6018 0.05% 72 3676 0.03% 73 2744 0.02% 74 2799 0.02% 75 2762 0.02% 76 2791 0.02% 77 3175 0.03% 78 3305 0.03% 79 3746 0.03% 80 3929 0.03% 81 4302 0.03% 82 5169 0.04% 83 5985 0.05% 84 7724 0.06% 85 9205 0.07% 86 10134 0.08% 87 12076 0.10% 88 13333 0.11% 89 14012 0.11% 90 13913 0.11% 91 13486 0.11% 92 14189 0.11% 93 14984 0.12% 94 15447 0.12% 95 16526 0.13% 96 16622 0.13% 97 17148 0.14% 98 17989 0.14% 99 19274 0.15% 100 20761 0.17% 101 21452 0.17% 102 22312 0.18% 103 24279 0.19% 104 25496 0.20% 105 27032 0.22% 106 28741 0.23% 107 29897 0.24% 108 31285 0.25% 109 30246 0.24% 110 31685 0.25% 111 32825 0.26% 112 35111 0.28% 113 39311 0.31% 114 42776 0.34% 115 45166 0.36% 116 45529 0.36% 117 43804 0.35% 118 42729 0.34% 119 43290 0.34% 120 46769 0.37% 121 45025 0.36% 122 48048 0.38% 123 50128 0.40% 124 51667 0.41% 125 52876 0.42% 126 54726 0.44% 127 54600 0.43% 128 52412 0.42% 129 57816 0.46% 130 56108 0.45% 131 56922 0.45% 132 59769 0.48% 133 61784 0.49% 134 62905 0.50% 135 63575 0.51% 136 64500 0.51% 137 65315 0.52% 138 70887 0.56% 139 73642 0.59% 140 75776 0.60% 141 77008 0.61% 142 90857 0.72% 143 92437 0.74% 144 99691 0.79% 145 115804 0.92% 146 138740 1.10% 147 173953 1.38% 148 253988 2.02% 149 510064 4.06% 150 2725228 21.69% 151 6085593 48.43% 12564600 reads passed initial QC criterion=sequence-density sequence-density=0.81 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=25 prefix-density=0.81 prefix-fanout=2.2 sequence=TTTCCTCTGGCT criterion=fanout-score sequence-density=0.66 sequence-density-rank=3 fanout-score=22.07 fanout-score-rank=1 prefix-density=7.47 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=sequence-density sequence-density=1.21 sequence-density-rank=1 fanout-score=4.74 fanout-score-rank=10 prefix-density=5.73 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.16 sequence-density-rank=18 fanout-score=93.55 fanout-score-rank=1 prefix-density=14.95 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR5578490 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 20:37:47 Started mapping on | Dec 09 20:37:47 Finished on | Dec 09 21:09:36 Mapping speed, Million of reads per hour | 23.69 Number of input reads | 12564600 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 1827655 Uniquely mapped reads % | 14.55% Average mapped length | 289.82 Number of splices: Total | 1145947 Number of splices: Annotated (sjdb) | 1080495 Number of splices: GT/AG | 1131335 Number of splices: GC/AG | 12858 Number of splices: AT/AC | 483 Number of splices: Non-canonical | 1271 Mismatch rate per base, % | 0.10% Deletion rate per base | 0.01% Deletion average length | 1.46 Insertion rate per base | 0.00% Insertion average length | 1.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 609049 % of reads mapped to multiple loci | 4.85% Number of reads mapped to too many loci | 133895 % of reads mapped to too many loci | 1.07% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 73.41% % of reads unmapped: other | 6.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10131119 10131119 10131119 N_multimapping 609049 609049 609049 N_noFeature 558949 1790741 567847 N_ambiguous 35677 505 7774 UnstrandedReadsAssigned:1233029 PositiveStrandReadsAssigned:36409 NegativeStrandReadsAssigned:1252034 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR5578490 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578490-trimmed-pair1.fastq SRR5578490-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 12,564,600 reads, 1,408,792 reads pseudoaligned [quant] estimated average fragment length: 211.444 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 907 rounds 52973 SRR5578490.ke.tsv 35125 SRR5578490.se.tsv 88098 total ==> SRR5578490.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 725.662 12.8052 10.4634 PNS24247 1044 833.556 0 0 PNS24249 1928 1717.56 5.19478 1.79339 PNS24246 1044 833.556 0 0 PNS24248 1044 833.556 0 0 PNS24244 1471 1260.56 0 0 PNS24243 293 114.688 0 0 KQK14069 1603 1392.56 568.966 242.267 KQK14071 474 271.778 0 0 ==> SRR5578490.se.tsv <== BRADI_1g14170v3 560 BRADI_1g53295v3 0 BRADI_1g59795v3 19 BRADI_1g07683v3 0 BRADI_1g00485v3 5 BRADI_1g20270v3 170 BRADI_1g74790v3 8 BRADI_1g09890v3 0 BRADI_1g77505v3 14 BRADI_1g48960v3 0 SRR5578490 completed mapping pipeline successfully