Starting /dee2/code/volunteer_pipeline.sh SRR5578492 current disk space = 1515292798976 free memory = 1604511692 SRR5578492 SRAfilesize d2b8b9ba58a7fffd5fc628442997c190 SRR5578492.sra SRR5578492.sra file validated SRR5578492 is paired end SRR5578492 is conventional basespace SRR5578492 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578492_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.30575 34.0 33.0 34.0 33.0 34.0 2 33.39025 34.0 33.0 34.0 33.0 34.0 3 33.37825 34.0 33.0 34.0 33.0 34.0 4 33.325 34.0 33.0 34.0 33.0 34.0 5 33.41675 34.0 34.0 34.0 33.0 34.0 6 37.13475 38.0 37.0 38.0 36.0 38.0 7 37.3195 38.0 38.0 38.0 37.0 38.0 8 37.48525 38.0 38.0 38.0 37.0 38.0 9 37.4285 38.0 38.0 38.0 37.0 38.0 10-14 37.423950000000005 38.0 38.0 38.0 37.0 38.0 15-19 37.4628 38.0 38.0 38.0 37.0 38.0 20-24 37.401199999999996 38.0 38.0 38.0 37.2 38.0 25-29 37.29485 38.0 38.0 38.0 37.2 38.0 30-34 37.208299999999994 38.0 38.0 38.0 37.0 38.0 35-39 37.18655 38.0 38.0 38.0 37.0 38.0 40-44 37.0616 38.0 38.0 38.0 36.8 38.0 45-49 37.040800000000004 38.0 38.0 38.0 36.6 38.0 50-54 37.0652 38.0 38.0 38.0 36.8 38.0 55-59 36.98105 38.0 38.0 38.0 36.4 38.0 60-64 36.9867 38.0 38.0 38.0 36.0 38.0 65-69 36.9212 38.0 38.0 38.0 36.0 38.0 70-74 36.7969 38.0 38.0 38.0 36.0 38.0 75-79 36.5153 38.0 38.0 38.0 35.4 38.0 80-84 36.4914 38.0 38.0 38.0 35.2 38.0 85-89 36.39855 38.0 38.0 38.0 35.0 38.0 90-94 36.31895 38.0 38.0 38.0 34.8 38.0 95-99 36.21555 38.0 38.0 38.0 34.4 38.0 100-104 36.08195 38.0 38.0 38.0 34.0 38.0 105-109 35.9387 38.0 38.0 38.0 33.8 38.0 110-114 35.96175 38.0 38.0 38.0 34.0 38.0 115-119 35.7165 38.0 38.0 38.0 33.2 38.0 120-124 35.674099999999996 38.0 38.0 38.0 33.0 38.0 125-129 35.57355 38.0 38.0 38.0 32.4 38.0 130-134 35.3656 38.0 37.4 38.0 31.4 38.0 135-139 35.0458 38.0 36.2 38.0 30.2 38.0 140-144 34.62885 38.0 36.0 38.0 27.6 38.0 145-149 34.46274999999999 38.0 36.0 38.0 27.8 38.0 150-151 31.170375 35.5 31.0 38.0 13.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 3.0 6 3.0 7 6.0 8 5.0 9 3.0 10 4.0 11 4.0 12 0.0 13 4.0 14 0.0 15 3.0 16 4.0 17 7.0 18 14.0 19 20.0 20 7.0 21 4.0 22 6.0 23 4.0 24 10.0 25 9.0 26 9.0 27 18.0 28 19.0 29 31.0 30 24.0 31 47.0 32 50.0 33 94.0 34 108.0 35 182.0 36 428.0 37 2869.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.8671679197995 11.528822055137844 10.100250626566416 31.50375939849624 2 27.575 12.825000000000001 29.825000000000003 29.775000000000002 3 24.775 16.825000000000003 24.425 33.975 4 24.975 25.025 23.625 26.375 5 27.875 27.075 25.974999999999998 19.075 6 22.75 33.225 25.3 18.725 7 15.9 25.25 41.425 17.424999999999997 8 20.775 25.025 32.125 22.075 9 19.6 23.200000000000003 34.65 22.55 10-14 23.715 27.465 26.365 22.455 15-19 22.38 26.515 26.884999999999998 24.22 20-24 22.945 25.074999999999996 28.249999999999996 23.73 25-29 21.84 27.150000000000002 27.700000000000003 23.31 30-34 22.275 27.529999999999998 27.275 22.919999999999998 35-39 22.765 26.095000000000002 27.24 23.9 40-44 23.865 27.005000000000003 26.179999999999996 22.95 45-49 23.535 27.195000000000004 26.71 22.56 50-54 24.455 26.419999999999998 26.005 23.119999999999997 55-59 23.215 25.979999999999997 27.384999999999998 23.419999999999998 60-64 21.759999999999998 27.51 26.369999999999997 24.36 65-69 22.1 27.99 26.009999999999998 23.9 70-74 22.78 29.07 24.715 23.435 75-79 22.945 26.490000000000002 26.055 24.51 80-84 24.035 27.07 25.555 23.34 85-89 24.03 26.325 26.08 23.565 90-94 23.544999999999998 26.255 26.169999999999998 24.03 95-99 22.49 25.8 27.47 24.240000000000002 100-104 23.75 26.400000000000002 25.56 24.29 105-109 22.965 27.425 25.94 23.669999999999998 110-114 22.465 26.484999999999996 25.585 25.465 115-119 22.830000000000002 27.865000000000002 25.155 24.15 120-124 23.991199559978 26.82134106705335 24.396219810990548 24.7912395619781 125-129 23.705000000000002 27.339999999999996 24.060000000000002 24.895 130-134 24.26 26.435 24.435000000000002 24.87 135-139 22.855 27.51 25.91 23.724999999999998 140-144 23.665 27.02 25.36 23.955000000000002 145-149 23.435 28.03 23.655 24.88 150-151 22.6875 25.474999999999998 25.887500000000003 25.95 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 1.0 5 1.0 6 1.0 7 1.0 8 2.0 9 2.5 10 2.0 11 0.5 12 0.0 13 0.5 14 1.0 15 0.5 16 0.5 17 2.0 18 1.5 19 0.5 20 1.5 21 1.0 22 0.5 23 0.5 24 1.0 25 3.0 26 2.5 27 2.0 28 3.5 29 17.5 30 32.0 31 40.0 32 43.0 33 46.5 34 60.0 35 81.0 36 111.0 37 160.5 38 177.5 39 144.5 40 144.5 41 137.0 42 116.5 43 111.5 44 109.5 45 121.0 46 134.5 47 144.5 48 169.5 49 168.5 50 148.0 51 159.0 52 162.5 53 162.5 54 147.0 55 127.5 56 124.0 57 105.5 58 90.5 59 88.5 60 65.5 61 45.5 62 37.5 63 31.5 64 34.5 65 25.0 66 21.0 67 23.5 68 18.0 69 15.5 70 15.0 71 10.0 72 6.5 73 6.0 74 3.5 75 5.5 76 7.5 77 4.5 78 2.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.97500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 87.52879236590984 66.5 2 6.679828891082592 10.15 3 2.4350115169463638 5.55 4 1.1846001974333662 3.5999999999999996 5 0.7568279039157618 2.875 6 0.3290556103981573 1.5 7 0.23033892727871008 1.225 8 0.29615004935834155 1.7999999999999998 9 0.09871668311944717 0.675 >10 0.46067785455742016 6.125 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 41 1.0250000000000001 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCTATCTCGTATGC 36 0.8999999999999999 TruSeq Adapter, Index 9 (97% over 36bp) GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 25 0.625 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 18 0.44999999999999996 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 16 0.4 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 15 0.375 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 14 0.35000000000000003 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 14 0.35000000000000003 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 12 0.3 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 12 0.3 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 12 0.3 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 10 0.25 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 10 0.25 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 10 0.25 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 9 0.22499999999999998 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 9 0.22499999999999998 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 9 0.22499999999999998 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 8 0.2 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 8 0.2 No Hit GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT 8 0.2 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 8 0.2 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 8 0.2 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 8 0.2 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 8 0.2 No Hit CAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAA 8 0.2 No Hit GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT 8 0.2 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 7 0.17500000000000002 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 7 0.17500000000000002 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 7 0.17500000000000002 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 7 0.17500000000000002 No Hit GATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGG 7 0.17500000000000002 No Hit GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG 7 0.17500000000000002 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 7 0.17500000000000002 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 6 0.15 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 6 0.15 No Hit GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA 6 0.15 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 6 0.15 No Hit GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC 6 0.15 No Hit ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT 6 0.15 No Hit CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT 6 0.15 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 6 0.15 No Hit GGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGC 6 0.15 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 6 0.15 No Hit CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG 5 0.125 No Hit GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA 5 0.125 No Hit GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT 5 0.125 No Hit GATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAG 5 0.125 No Hit CTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACA 5 0.125 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 5 0.125 No Hit GTACAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTC 5 0.125 No Hit GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG 5 0.125 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 5 0.125 No Hit CAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCA 5 0.125 No Hit TGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT 5 0.125 No Hit TTTTTTTTTAAGGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCG 5 0.125 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 5 0.125 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 5 0.125 No Hit TGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATAT 5 0.125 No Hit ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 5 0.125 No Hit GCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAA 5 0.125 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 5 0.125 No Hit GTTTCATCAATGGCACTCTCTCACAGCCAATAACTTCAACAACTTCCCTA 5 0.125 No Hit CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC 5 0.125 No Hit CTCAAAGAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACC 5 0.125 No Hit CCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATA 5 0.125 No Hit CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.0625 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1375 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.16249999999999998 0.0 0.0 0.0 0.0 74-75 0.2375 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.3625 0.0 0.0 0.0 0.0 80-81 0.5 0.0 0.0 0.0 0.0 82-83 0.7 0.0 0.0 0.0 0.0 84-85 0.85 0.0 0.0 0.0 0.0 86-87 0.975 0.0 0.0 0.0 0.0 88-89 1.3 0.0 0.0 0.0 0.0 90-91 1.6625 0.0 0.0 0.0 0.0 92-93 1.8875000000000002 0.0 0.0 0.0 0.0 94-95 2.0999999999999996 0.0 0.0 0.0 0.0 96-97 2.5375 0.0 0.0 0.0 0.0 98-99 3.0125 0.0 0.0 0.0 0.0 100-101 3.5 0.0 0.0 0.0 0.0 102-103 3.8 0.0 0.0 0.0 0.0 104-105 4.2125 0.0 0.0 0.0 0.0 106-107 4.775 0.0 0.0 0.0 0.0 108-109 5.425000000000001 0.0 0.0 0.0 0.0 110-111 6.074999999999999 0.0 0.0 0.0 0.0 112-113 6.699999999999999 0.0 0.0 0.0 0.0 114-115 7.2875 0.0 0.0 0.0 0.0 116-117 8.15 0.0 0.0 0.0 0.0 118-119 9.1375 0.0 0.0 0.0 0.0 120-121 10.0 0.0 0.0 0.0 0.0 122-123 11.075 0.0 0.0 0.0 0.0 124-125 11.9875 0.0 0.0 0.0 0.0 126-127 13.05 0.0 0.0 0.0 0.0 128-129 14.225 0.0 0.0 0.0 0.0 130-131 15.212499999999999 0.0 0.0 0.0 0.0 132-133 16.275 0.0 0.0 0.0 0.0 134-135 17.4375 0.0 0.0 0.0 0.0 136-137 18.4 0.0 0.0 0.0 0.0 138-139 19.35 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAATCC 10 0.006830828 145.0 3 TATGAGT 45 1.0549343E-4 64.44444 6 TTATGAG 35 0.0033124194 62.14286 5 GGTTATG 35 0.0033124194 62.14286 3 GAGTAGG 35 0.0033124194 62.14286 9 GTTATGA 35 0.0033124194 62.14286 4 ATGAGTA 35 0.0033124194 62.14286 7 GGGTTAT 40 0.005621335 54.375 2 ATAATTG 40 0.005621335 54.375 145 GGGGTTA 40 0.005621335 54.375 1 TGAGTAG 45 0.008957279 48.333332 8 CACGTCT 95 0.007278115 15.263159 145 CTCCAGT 70 7.343502E-4 14.5 140-144 AACTCCA 85 0.0031733946 11.941176 140-144 CTGAACT 85 0.0031733946 11.941176 135-139 GTCTGAA 90 0.0048656333 11.277777 135-139 >>END_MODULE SRR5578492 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578492_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.719 33.0 32.0 33.0 28.0 34.0 2 31.808 33.0 32.0 34.0 28.0 34.0 3 31.9345 33.0 33.0 34.0 30.0 34.0 4 31.67 33.0 33.0 34.0 29.0 34.0 5 31.76025 33.0 33.0 34.0 29.0 34.0 6 35.89725 38.0 37.0 38.0 31.0 38.0 7 35.6385 38.0 37.0 38.0 29.0 38.0 8 35.7155 38.0 37.0 38.0 29.0 38.0 9 35.545 38.0 37.0 38.0 29.0 38.0 10-14 35.4705 38.0 37.0 38.0 29.0 38.0 15-19 35.36555 38.0 37.0 38.0 28.6 38.0 20-24 35.201049999999995 38.0 37.0 38.0 28.2 38.0 25-29 35.08635 38.0 36.8 38.0 27.6 38.0 30-34 34.844550000000005 38.0 36.0 38.0 27.0 38.0 35-39 34.61125 38.0 36.0 38.0 26.2 38.0 40-44 34.415800000000004 38.0 35.6 38.0 25.0 38.0 45-49 34.143649999999994 38.0 34.8 38.0 23.0 38.0 50-54 33.7554 38.0 34.0 38.0 17.6 38.0 55-59 33.55820000000001 38.0 34.0 38.0 16.0 38.0 60-64 33.417500000000004 38.0 34.0 38.0 16.0 38.0 65-69 32.8288 38.0 33.2 38.0 16.0 38.0 70-74 32.19845 37.0 30.8 38.0 15.0 38.0 75-79 31.642000000000003 37.0 29.2 38.0 15.0 38.0 80-84 31.0953 37.0 28.4 38.0 14.8 38.0 85-89 30.49275 36.4 26.8 38.0 14.2 38.0 90-94 30.004900000000003 36.0 26.0 38.0 13.0 38.0 95-99 29.081349999999997 35.0 23.4 38.0 8.6 38.0 100-104 28.0217 34.0 18.6 38.0 2.0 38.0 105-109 26.96105 34.0 15.0 38.0 2.0 38.0 110-114 25.8257 33.0 15.0 37.2 2.0 38.0 115-119 25.1565 32.6 14.0 37.6 2.0 38.0 120-124 24.0026 30.2 13.2 36.8 2.0 38.0 125-129 22.51755 27.6 4.2 36.0 2.0 38.0 130-134 20.708900000000003 23.2 2.0 35.2 2.0 38.0 135-139 19.022399999999998 18.4 2.0 34.6 2.0 38.0 140-144 16.9297 13.6 2.0 33.8 2.0 38.0 145-149 15.07095 4.2 2.0 34.0 2.0 38.0 150-151 11.22625 2.0 2.0 24.0 2.0 36.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29.0 3 20.0 4 8.0 5 15.0 6 10.0 7 5.0 8 13.0 9 9.0 10 19.0 11 25.0 12 24.0 13 27.0 14 24.0 15 38.0 16 32.0 17 48.0 18 57.0 19 58.0 20 52.0 21 65.0 22 95.0 23 84.0 24 107.0 25 134.0 26 127.0 27 151.0 28 188.0 29 168.0 30 209.0 31 245.0 32 298.0 33 342.0 34 352.0 35 414.0 36 370.0 37 138.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.699999999999996 19.125 13.075000000000001 26.1 2 28.1 24.075 25.650000000000002 22.175 3 22.061030515257627 24.437218609304654 28.96448224112056 24.537268634317158 4 25.494120590442833 32.02401801351013 21.66624968726545 20.815611708781585 5 27.031757939484873 30.932733183295824 21.405351337834457 20.630157539384847 6 22.828535669586984 34.34292866082603 21.00125156445557 21.827284105131415 7 21.101376720901126 22.478097622027533 33.69211514392991 22.728410513141426 8 23.171342685370742 24.9749498997996 24.874749498997996 26.978957915831664 9 24.242424242424242 24.34259954921112 26.120711244678184 25.29426496368645 10-14 25.134011322078052 26.727117879865737 22.769400330644757 25.369470467411453 15-19 25.50345656747821 27.0814547640517 24.10079150385733 23.314297164612764 20-24 25.262999699428917 26.916140667267808 24.13585813044785 23.685001502855425 25-29 26.387219551282055 26.257011217948715 24.01842948717949 23.337339743589745 30-34 27.13240453041997 26.255387390999296 23.82980855968728 22.782399518893452 35-39 25.67919799498747 25.428571428571427 24.87218045112782 24.020050125313283 40-44 26.12486221064235 25.53362060326686 24.817115943481312 23.52440124260948 45-49 25.79013273228149 25.1740545955422 25.77009767092412 23.26571500125219 50-54 24.655543864923093 24.460143293752193 26.77488852146901 24.109424319855705 55-59 23.763963332164504 26.36878224715724 26.984922105895908 22.882332314782346 60-64 22.949013322648504 26.394871281178002 26.870680156265653 23.785435239907844 65-69 23.375907838717755 27.473077886301027 26.316053092912593 22.834961182068618 70-74 22.822491730981255 27.347900170391902 25.939661220807857 23.889946877818986 75-79 22.973988873853557 28.376685210244073 24.823334836866636 23.82599107903573 80-84 23.04029671210906 27.97213311948677 25.651563753007217 23.336006415396955 85-89 22.657580919931856 28.154123659685336 25.894378194207835 23.29391722617497 90-94 23.91097298110181 28.19690210035591 25.45992280314803 22.432202115394258 95-99 23.042998897464166 29.197153452941766 25.373358725067657 22.38648892452641 100-104 24.040677286845007 28.829776575493437 24.246067528303776 22.88347860935778 105-109 23.90116774419887 29.484288076980903 23.991379742394628 22.6231644364256 110-114 23.377274322089118 29.652648990025565 23.542679564934087 23.42739712295123 115-119 23.792101042502004 30.839013632718526 23.10545308740978 22.26343223736969 120-124 24.229670825191644 29.355178115136027 23.73866426173656 22.67648679793577 125-129 24.607749761892826 30.96897087573312 22.07629455110532 22.346984811268737 130-134 25.174194195197753 29.04907514161111 23.770615068424483 22.006115594766655 135-139 24.804648367060707 30.214385894610295 22.876177118813864 22.10478861951513 140-144 26.62861148665565 29.813229182314355 22.46757798808272 21.090581342947274 145-149 26.097194388777556 29.914829659318638 22.720440881763526 21.26753507014028 150-151 26.95390781563126 31.337675350701407 21.455410821643287 20.25300601202405 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.5 3 1.5 4 1.5 5 1.0 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 1.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.5 18 0.5 19 0.0 20 0.5 21 1.0 22 1.5 23 1.5 24 2.5 25 3.5 26 2.0 27 4.5 28 8.0 29 9.5 30 14.5 31 21.5 32 30.5 33 37.0 34 48.0 35 58.5 36 75.5 37 113.0 38 149.5 39 161.5 40 206.0 41 181.0 42 108.0 43 107.0 44 100.5 45 123.5 46 132.5 47 126.0 48 156.0 49 158.5 50 142.0 51 131.0 52 156.5 53 188.5 54 184.5 55 170.0 56 140.5 57 110.5 58 88.0 59 80.5 60 75.0 61 54.5 62 35.5 63 34.5 64 40.5 65 29.0 66 23.0 67 27.0 68 23.0 69 24.0 70 22.0 71 13.0 72 11.0 73 11.5 74 9.5 75 7.5 76 5.0 77 3.5 78 1.5 79 0.5 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.05 4 0.075 5 0.025 6 0.125 7 0.125 8 0.2 9 0.17500000000000002 10-14 0.19499999999999998 15-19 0.19 20-24 0.19 25-29 0.16 30-34 0.22999999999999998 35-39 0.25 40-44 0.21 45-49 0.17500000000000002 50-54 0.20500000000000002 55-59 0.185 60-64 0.16999999999999998 65-69 0.17500000000000002 70-74 0.22999999999999998 75-79 0.23500000000000001 80-84 0.24 85-89 0.21 90-94 0.255 95-99 0.22999999999999998 100-104 0.19 105-109 0.23500000000000001 110-114 0.245 115-119 0.24 120-124 0.20500000000000002 125-129 0.255 130-134 0.255 135-139 0.18 140-144 0.145 145-149 0.2 150-151 0.2 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.625 #Duplication Level Percentage of deduplicated Percentage of total 1 89.03752039151713 68.22500000000001 2 5.970636215334421 9.15 3 1.9902120717781402 4.575 4 1.2398042414355628 3.8 5 0.48939641109298526 1.875 6 0.1631321370309951 0.75 7 0.1631321370309951 0.8750000000000001 8 0.09787928221859706 0.6 9 0.13050570962479607 0.8999999999999999 >10 0.7177814029363785 9.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 28 0.7000000000000001 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 27 0.675 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 27 0.675 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 24 0.6 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 24 0.6 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 23 0.575 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 19 0.475 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 19 0.475 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 19 0.475 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 18 0.44999999999999996 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 16 0.4 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 14 0.35000000000000003 Illumina Single End PCR Primer 1 (100% over 50bp) AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 13 0.325 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 12 0.3 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 12 0.3 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 12 0.3 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 11 0.27499999999999997 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 11 0.27499999999999997 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 11 0.27499999999999997 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 10 0.25 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 10 0.25 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 10 0.25 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 9 0.22499999999999998 No Hit GCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAG 9 0.22499999999999998 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 9 0.22499999999999998 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 9 0.22499999999999998 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 8 0.2 No Hit CTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGATC 8 0.2 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 8 0.2 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 7 0.17500000000000002 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 7 0.17500000000000002 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 7 0.17500000000000002 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 7 0.17500000000000002 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 7 0.17500000000000002 No Hit AAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT 6 0.15 No Hit GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG 6 0.15 No Hit GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG 6 0.15 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 6 0.15 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 6 0.15 No Hit GGCAGAGGCGTTTGTATCTGCCATTATAAAGAAGTTTCCTCCAGCAACTC 5 0.125 No Hit GCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAG 5 0.125 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 5 0.125 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 5 0.125 No Hit GTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGG 5 0.125 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 5 0.125 No Hit GGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTA 5 0.125 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 5 0.125 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 5 0.125 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 5 0.125 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 5 0.125 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 5 0.125 No Hit CGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGT 5 0.125 No Hit GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC 5 0.125 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.16249999999999998 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.2625 0.0 0.0 0.0 0.0 80-81 0.36250000000000004 0.0 0.0 0.0 0.0 82-83 0.55 0.0 0.0 0.0 0.0 84-85 0.6875 0.0 0.0 0.0 0.0 86-87 0.8 0.0 0.0 0.0 0.0 88-89 1.075 0.0 0.0 0.0 0.0 90-91 1.325 0.0 0.0 0.0 0.0 92-93 1.4125 0.0 0.0 0.0 0.0 94-95 1.575 0.0 0.0 0.0 0.0 96-97 1.8625 0.0 0.0 0.0 0.0 98-99 2.2750000000000004 0.0 0.0 0.0 0.0 100-101 2.5999999999999996 0.0 0.0 0.0 0.0 102-103 2.8125 0.0 0.0 0.0 0.0 104-105 3.075 0.0 0.0 0.0 0.0 106-107 3.45 0.0 0.0 0.0 0.0 108-109 3.8375 0.0 0.0 0.0 0.0 110-111 4.175000000000001 0.0 0.0 0.0 0.0 112-113 4.550000000000001 0.0 0.0 0.0 0.0 114-115 4.9125 0.0 0.0 0.0 0.0 116-117 5.4875 0.0 0.0 0.0 0.0 118-119 6.0875 0.0 0.0 0.0 0.0 120-121 6.525 0.0 0.0 0.0 0.0 122-123 7.1625 0.0 0.0 0.0 0.0 124-125 7.762499999999999 0.0 0.0 0.0 0.0 126-127 8.3625 0.0 0.0 0.0 0.0 128-129 9.0 0.0 0.0 0.0 0.0 130-131 9.475000000000001 0.0 0.0 0.0 0.0 132-133 10.025 0.0 0.0 0.0 0.0 134-135 10.475 0.0 0.0 0.0 0.0 136-137 10.825 0.0 0.0 0.0 0.0 138-139 11.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGGG 10 0.006830828 145.0 3 AATTAGG 10 0.006830828 145.0 2 CCCAAGC 10 0.006830828 145.0 1 TAATTAG 10 0.006830828 145.0 1 CCAAGCT 10 0.006830828 145.0 2 AAGCTGC 10 0.006830828 145.0 4 GCTCACA 10 0.006830828 145.0 9 CAAGCTG 10 0.006830828 145.0 3 TAGGGAA 45 6.5511256E-4 19.333332 135-139 TGTAGGG 55 1.1668232E-4 18.454546 135-139 TCGTGTA 55 0.0025160722 15.818182 130-134 CGTGTAG 65 0.0076375785 13.384615 130-134 >>END_MODULE Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830019 spots for SRR5578492.sra Written 830019 spots for SRR5578492.sra Read 830023 spots for SRR5578492.sra Written 830023 spots for SRR5578492.sra SRR ids: ['SRR5578492.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_52b3pyg6 SRR5578492.sra spots: 16600384 blocks: [[1, 830019], [830020, 1660038], [1660039, 2490057], [2490058, 3320076], [3320077, 4150095], [4150096, 4980114], [4980115, 5810133], [5810134, 6640152], [6640153, 7470171], [7470172, 8300190], [8300191, 9130209], [9130210, 9960228], [9960229, 10790247], [10790248, 11620266], [11620267, 12450285], [12450286, 13280304], [13280305, 14110323], [14110324, 14940342], [14940343, 15770361], [15770362, 16600384]] SRR5578492 file size 5603625 SRR5578492 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578492 SRR5578492_1.fastq SRR5578492_2.fastq Input file: SRR5578492_1.fastq Paired file: SRR5578492_2.fastq trimmed: SRR5578492-trimmed-pair1.fastq, SRR5578492-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 03:15:32 2024 >> started Thu Dec 12 03:15:51 2024 >> done (18.846s) 16600384 read pairs processed; of these: 62927 ( 0.38%) short read pairs filtered out after trimming by size control 244347 ( 1.47%) empty read pairs filtered out after trimming by size control 16293110 (98.15%) read pairs available; of these: 9334463 (57.29%) trimmed read pairs available after processing 6958647 (42.71%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 15 0.00% 20 15 0.00% 21 34 0.00% 22 27 0.00% 23 38 0.00% 24 53 0.00% 25 36 0.00% 26 54 0.00% 27 45 0.00% 28 56 0.00% 29 42 0.00% 30 68 0.00% 31 62 0.00% 32 47 0.00% 33 50 0.00% 34 46 0.00% 35 58 0.00% 36 59 0.00% 37 67 0.00% 38 74 0.00% 39 90 0.00% 40 103 0.00% 41 105 0.00% 42 120 0.00% 43 161 0.00% 44 210 0.00% 45 249 0.00% 46 292 0.00% 47 295 0.00% 48 348 0.00% 49 383 0.00% 50 398 0.00% 51 468 0.00% 52 522 0.00% 53 574 0.00% 54 617 0.00% 55 736 0.00% 56 774 0.00% 57 851 0.01% 58 972 0.01% 59 1023 0.01% 60 1181 0.01% 61 1294 0.01% 62 1594 0.01% 63 1726 0.01% 64 2038 0.01% 65 2397 0.01% 66 2999 0.02% 67 3642 0.02% 68 4958 0.03% 69 12656 0.08% 70 16793 0.10% 71 8277 0.05% 72 6317 0.04% 73 6390 0.04% 74 6844 0.04% 75 7662 0.05% 76 8122 0.05% 77 8909 0.05% 78 9988 0.06% 79 11238 0.07% 80 12160 0.07% 81 13235 0.08% 82 15416 0.09% 83 17065 0.10% 84 21135 0.13% 85 24535 0.15% 86 25966 0.16% 87 28228 0.17% 88 30454 0.19% 89 31463 0.19% 90 32971 0.20% 91 33742 0.21% 92 34875 0.21% 93 37110 0.23% 94 38143 0.23% 95 40064 0.25% 96 42235 0.26% 97 43821 0.27% 98 45147 0.28% 99 47321 0.29% 100 51296 0.31% 101 52149 0.32% 102 53758 0.33% 103 56684 0.35% 104 60108 0.37% 105 63068 0.39% 106 66330 0.41% 107 67437 0.41% 108 69951 0.43% 109 68767 0.42% 110 69499 0.43% 111 72683 0.45% 112 75767 0.47% 113 82179 0.50% 114 85685 0.53% 115 91103 0.56% 116 88811 0.55% 117 88111 0.54% 118 87464 0.54% 119 88596 0.54% 120 93468 0.57% 121 92699 0.57% 122 97314 0.60% 123 99605 0.61% 124 103166 0.63% 125 103772 0.64% 126 107371 0.66% 127 106114 0.65% 128 104271 0.64% 129 110047 0.68% 130 109005 0.67% 131 109121 0.67% 132 112010 0.69% 133 115189 0.71% 134 118292 0.73% 135 119122 0.73% 136 119654 0.73% 137 122472 0.75% 138 129542 0.80% 139 133717 0.82% 140 137676 0.84% 141 140453 0.86% 142 158371 0.97% 143 164586 1.01% 144 175491 1.08% 145 198436 1.22% 146 227790 1.40% 147 270700 1.66% 148 365095 2.24% 149 602219 3.70% 150 2497892 15.33% 151 6958647 42.71% 16293110 reads passed initial QC criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=21.71 fanout-score-rank=2 prefix-density=7.17 prefix-fanout=2.0 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.10 sequence-density-rank=24 fanout-score=23.61 fanout-score-rank=1 prefix-density=2.13 prefix-fanout=1.1 sequence=TTGTCTTTAAACGCTCACCTATCATTTGCTAAGGCAGTTAAAGAATGTGGAACATTCATGGGAACTGGTGAAGGAGGATTGCCAAAAGCTCTCTACCCTTATGCAGACCACATAATTACCCAAGTTGCAAGTGGAAGATTTGGAGTTAATGAAGAGTATCTTATGAAAGGTTCTGCAATAGAGATTAAAATAGGGCAGGGAGCTAAGCCTGGAATTGGAGGGCACTTACCTGGAGAGAAGGTTACAGCAGAAATTTCAGCAACAAGAATGATTCCTGAGGGAAGTGATGCTATCTCACCAGCTCCTCACCATGACATTTACTCAATTGAGGATTTAGCTCAATTAGTTAGAAGTTTGAAAGAAGCAACAAGATGGAAAAAGCCAGTGTTTGTTAAAATTGCAGCTGTCCATAATGCTCCAGCTATTGCTGTTGGAATAGCAACAAGTGATGCTGACGCAGTTGTTATAGATGGATATAAAGGAGGGACAGGGGCAGCACCAAAGGTATTCA criterion=sequence-density sequence-density=1.05 sequence-density-rank=1 fanout-score=4.80 fanout-score-rank=8 prefix-density=4.95 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.11 sequence-density-rank=18 fanout-score=108.48 fanout-score-rank=1 prefix-density=11.12 prefix-fanout=1.0 sequence=CCCAACTATACAAAAGAATATCC SRR5578492 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 12 03:17:34 Started mapping on | Dec 12 03:17:34 Finished on | Dec 12 03:49:09 Mapping speed, Million of reads per hour | 30.95 Number of input reads | 16293110 Average input read length | 281 UNIQUE READS: Uniquely mapped reads number | 5563870 Uniquely mapped reads % | 34.15% Average mapped length | 279.08 Number of splices: Total | 4438884 Number of splices: Annotated (sjdb) | 4168703 Number of splices: GT/AG | 4379662 Number of splices: GC/AG | 51933 Number of splices: AT/AC | 2854 Number of splices: Non-canonical | 4435 Mismatch rate per base, % | 0.15% Deletion rate per base | 0.00% Deletion average length | 1.42 Insertion rate per base | 0.00% Insertion average length | 1.09 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 201707 % of reads mapped to multiple loci | 1.24% Number of reads mapped to too many loci | 58524 % of reads mapped to too many loci | 0.36% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 62.72% % of reads unmapped: other | 1.54% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10540642 10540642 10540642 N_multimapping 201707 201707 201707 N_noFeature 183555 5399327 225253 N_ambiguous 137067 523 14946 UnstrandedReadsAssigned:5243248 PositiveStrandReadsAssigned:164020 NegativeStrandReadsAssigned:5323671 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=135 echo kmer=131 SRR5578492 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578492-trimmed-pair1.fastq SRR5578492-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,293,110 reads, 5,419,250 reads pseudoaligned [quant] estimated average fragment length: 196.48 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,016 rounds 52973 SRR5578492.ke.tsv 35125 SRR5578492.se.tsv 88098 total ==> SRR5578492.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 740.633 0 0 PNS24247 1044 848.52 0 0 PNS24249 1928 1732.52 14.7688 1.91907 PNS24246 1044 848.52 0 0 PNS24248 1044 848.52 0 0 PNS24244 1471 1275.52 104.231 18.3964 PNS24243 293 123.212 0 0 KQK14069 1603 1407.52 159.07 25.4424 KQK14071 474 284.524 1.23271 0.975362 ==> SRR5578492.se.tsv <== BRADI_1g14170v3 164 BRADI_1g53295v3 11 BRADI_1g59795v3 67 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 295 BRADI_1g74790v3 21 BRADI_1g09890v3 3 BRADI_1g77505v3 90 BRADI_1g48960v3 0 SRR5578492 completed mapping pipeline successfully