Starting /dee2/code/volunteer_pipeline.sh SRR5578497 current disk space = 1515234414592 free memory = 1598669064 SRR5578497 SRAfilesize 156aeb1f08ae19b6a014dcf5fb6e76ba SRR5578497.sra SRR5578497.sra file validated SRR5578497 is paired end SRR5578497 is conventional basespace SRR5578497 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578497_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.29375 34.0 33.0 34.0 33.0 34.0 2 33.433 34.0 34.0 34.0 33.0 34.0 3 33.431 34.0 33.0 34.0 33.0 34.0 4 33.40225 34.0 34.0 34.0 33.0 34.0 5 33.41325 34.0 34.0 34.0 33.0 34.0 6 36.98725 38.0 37.0 38.0 36.0 38.0 7 37.32975 38.0 38.0 38.0 37.0 38.0 8 37.44375 38.0 38.0 38.0 37.0 38.0 9 37.4915 38.0 38.0 38.0 37.0 38.0 10-14 37.495549999999994 38.0 38.0 38.0 37.4 38.0 15-19 37.4679 38.0 38.0 38.0 37.2 38.0 20-24 37.4507 38.0 38.0 38.0 37.6 38.0 25-29 37.35425 38.0 38.0 38.0 37.0 38.0 30-34 37.3382 38.0 38.0 38.0 37.4 38.0 35-39 37.24720000000001 38.0 38.0 38.0 37.0 38.0 40-44 37.1152 38.0 38.0 38.0 37.0 38.0 45-49 37.128099999999996 38.0 38.0 38.0 36.8 38.0 50-54 37.12564999999999 38.0 38.0 38.0 36.8 38.0 55-59 37.068200000000004 38.0 38.0 38.0 36.0 38.0 60-64 37.05375 38.0 38.0 38.0 36.2 38.0 65-69 36.945449999999994 38.0 38.0 38.0 36.0 38.0 70-74 36.8236 38.0 38.0 38.0 36.0 38.0 75-79 36.608349999999994 38.0 38.0 38.0 35.8 38.0 80-84 36.443 38.0 38.0 38.0 35.0 38.0 85-89 36.39705 38.0 38.0 38.0 34.8 38.0 90-94 36.2565 38.0 38.0 38.0 34.4 38.0 95-99 36.14425 38.0 38.0 38.0 34.0 38.0 100-104 36.106899999999996 38.0 38.0 38.0 34.0 38.0 105-109 35.978699999999996 38.0 38.0 38.0 34.0 38.0 110-114 35.87065 38.0 38.0 38.0 33.6 38.0 115-119 35.655899999999995 38.0 38.0 38.0 32.8 38.0 120-124 35.56824999999999 38.0 38.0 38.0 32.6 38.0 125-129 35.448 38.0 37.6 38.0 31.6 38.0 130-134 35.29445 38.0 36.6 38.0 31.0 38.0 135-139 34.9573 38.0 36.2 38.0 29.6 38.0 140-144 34.550850000000004 38.0 35.6 38.0 27.4 38.0 145-149 34.24375 38.0 35.4 38.0 26.2 38.0 150-151 30.90625 35.5 30.5 38.0 11.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 7.0 8 4.0 9 2.0 10 0.0 11 2.0 12 4.0 13 4.0 14 1.0 15 1.0 16 5.0 17 4.0 18 18.0 19 24.0 20 4.0 21 7.0 22 7.0 23 10.0 24 6.0 25 9.0 26 10.0 27 19.0 28 25.0 29 37.0 30 37.0 31 38.0 32 60.0 33 72.0 34 112.0 35 180.0 36 410.0 37 2879.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.620015048908954 11.863556558816153 9.932279909706546 33.58414848256835 2 26.8 14.075 28.525 30.599999999999998 3 23.549999999999997 17.7 23.9 34.849999999999994 4 26.924999999999997 23.674999999999997 22.075 27.325 5 29.025000000000002 25.974999999999998 24.0 21.0 6 23.674999999999997 31.525 24.425 20.375 7 17.175 24.25 40.775 17.8 8 19.2 24.55 30.75 25.5 9 20.4 23.0 33.675 22.925 10-14 23.849999999999998 27.215 25.995 22.939999999999998 15-19 23.69 26.045 25.814999999999998 24.45 20-24 23.317331733173315 25.657565756575657 26.292629262926294 24.732473247324734 25-29 23.0 26.245 26.57 24.185000000000002 30-34 22.86 26.415 25.805 24.92 35-39 23.095 25.55 26.19 25.165 40-44 24.42 25.39 25.290000000000003 24.9 45-49 23.765 26.14 26.025 24.07 50-54 25.064999999999998 25.645 24.66 24.63 55-59 23.857385738573857 25.57255725572557 25.557555755575557 25.012501250125013 60-64 23.135 26.61 25.195 25.06 65-69 23.265 27.21 24.58 24.945 70-74 24.02 27.0 23.735 25.245 75-79 23.615 25.55 25.130000000000003 25.705 80-84 24.84 25.91 24.905 24.345 85-89 24.72 25.27 25.535000000000004 24.474999999999998 90-94 24.68 25.575 24.66 25.085 95-99 24.08 25.085 25.650000000000002 25.185000000000002 100-104 24.91 25.240000000000002 24.62 25.230000000000004 105-109 24.795 26.085 24.605 24.515 110-114 23.935000000000002 26.009999999999998 23.945 26.11 115-119 24.34621731086554 26.21131056552828 24.131206560328017 25.311265563278162 120-124 24.832483248324834 25.312531253125314 23.512351235123514 26.34263426342634 125-129 24.535 25.53 23.294999999999998 26.640000000000004 130-134 25.369999999999997 24.685000000000002 23.7 26.245 135-139 24.4 26.625 23.885 25.09 140-144 24.66 26.215 23.535 25.590000000000003 145-149 24.38 25.88 23.0 26.740000000000002 150-151 23.6375 25.825 23.200000000000003 27.3375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 0.5 3 1.0 4 1.5 5 1.0 6 1.5 7 2.0 8 1.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 1.5 15 1.5 16 0.5 17 0.0 18 1.0 19 1.0 20 0.0 21 0.0 22 1.0 23 1.0 24 0.5 25 2.0 26 1.5 27 0.5 28 3.0 29 16.5 30 23.5 31 30.5 32 39.5 33 43.5 34 61.5 35 70.5 36 83.0 37 126.5 38 141.5 39 116.5 40 114.5 41 118.5 42 114.5 43 117.0 44 119.0 45 126.0 46 139.0 47 157.0 48 159.5 49 155.5 50 145.0 51 125.0 52 134.0 53 141.5 54 121.5 55 113.0 56 108.5 57 99.0 58 104.0 59 101.0 60 85.0 61 72.5 62 67.5 63 69.0 64 66.5 65 60.5 66 49.5 67 40.0 68 36.5 69 30.5 70 28.0 71 24.5 72 18.0 73 16.5 74 16.0 75 10.0 76 6.5 77 5.5 78 3.0 79 1.0 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.01 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.005 120-124 0.01 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.075 #Duplication Level Percentage of deduplicated Percentage of total 1 92.03643843667354 78.3 2 5.02497796062298 8.55 3 1.1754334410813987 3.0 4 0.7640317367029091 2.6 5 0.32324419629738466 1.375 6 0.2057008521892448 1.05 7 0.11754334410813988 0.7000000000000001 8 0.08815750808110491 0.6 9 0.02938583602703497 0.22499999999999998 >10 0.23508668821627976 3.5999999999999996 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTTATCTCGTATGC 42 1.05 TruSeq Adapter, Index 23 (97% over 38bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 27 0.675 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 16 0.4 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 15 0.375 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 13 0.325 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 11 0.27499999999999997 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 10 0.25 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 10 0.25 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 9 0.22499999999999998 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 8 0.2 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 8 0.2 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 8 0.2 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 7 0.17500000000000002 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 7 0.17500000000000002 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 7 0.17500000000000002 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 7 0.17500000000000002 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 6 0.15 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 6 0.15 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 6 0.15 No Hit CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT 6 0.15 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 6 0.15 No Hit GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG 6 0.15 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 6 0.15 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 5 0.125 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 5 0.125 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 5 0.125 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 5 0.125 No Hit GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA 5 0.125 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 5 0.125 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 5 0.125 No Hit GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT 5 0.125 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 5 0.125 No Hit TGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATC 5 0.125 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.48750000000000004 0.0 0.0 0.0 0.0 86-87 0.6000000000000001 0.0 0.0 0.0 0.0 88-89 0.8 0.0 0.0 0.0 0.0 90-91 0.9875 0.0 0.0 0.0 0.0 92-93 1.1625 0.0 0.0 0.0 0.0 94-95 1.3624999999999998 0.0 0.0 0.0 0.0 96-97 1.6125 0.0 0.0 0.0 0.0 98-99 1.875 0.0 0.0 0.0 0.0 100-101 2.1375 0.0 0.0 0.0 0.0 102-103 2.575 0.0 0.0 0.0 0.0 104-105 3.05 0.0 0.0 0.0 0.0 106-107 3.675 0.0 0.0 0.0 0.0 108-109 4.2625 0.0 0.0 0.0 0.0 110-111 4.6125 0.0 0.0 0.0 0.0 112-113 5.2 0.0 0.0 0.0 0.0 114-115 5.925 0.0 0.0 0.0 0.0 116-117 6.525 0.0 0.0 0.0 0.0 118-119 7.0875 0.0 0.0 0.0 0.0 120-121 7.85 0.0 0.0 0.0 0.0 122-123 8.7375 0.0 0.0 0.0 0.0 124-125 9.55 0.0 0.0 0.0 0.0 126-127 10.3125 0.0 0.0 0.0 0.0 128-129 11.2 0.0 0.0 0.0 0.0 130-131 12.1 0.0 0.0 0.0 0.0 132-133 12.975 0.0 0.0 0.0 0.0 134-135 14.125 0.0 0.0 0.0 0.0 136-137 15.4375 0.0 0.0 0.0 0.0 138-139 16.2625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGGA 10 0.006830828 145.0 6 TTTTTTA 10 0.006830828 145.0 4 >>END_MODULE SRR5578497 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578497_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75775 33.0 32.0 33.0 28.0 34.0 2 31.854 33.0 33.0 34.0 28.0 34.0 3 31.9395 33.0 33.0 34.0 30.0 34.0 4 31.70475 33.0 33.0 34.0 29.0 34.0 5 31.7405 33.0 33.0 34.0 29.0 34.0 6 35.787 38.0 37.0 38.0 31.0 38.0 7 35.60575 38.0 37.0 38.0 29.0 38.0 8 35.633 38.0 37.0 38.0 31.0 38.0 9 35.5865 38.0 37.0 38.0 29.0 38.0 10-14 35.46985 38.0 37.0 38.0 29.2 38.0 15-19 35.278800000000004 38.0 37.0 38.0 28.6 38.0 20-24 35.25435 38.0 37.0 38.0 28.8 38.0 25-29 35.02505000000001 38.0 36.8 38.0 27.8 38.0 30-34 34.839299999999994 38.0 36.0 38.0 27.4 38.0 35-39 34.69635 38.0 36.0 38.0 27.0 38.0 40-44 34.4264 38.0 36.0 38.0 25.2 38.0 45-49 34.14685 38.0 35.0 38.0 24.8 38.0 50-54 33.80425 38.0 34.4 38.0 17.6 38.0 55-59 33.5507 38.0 34.0 38.0 16.0 38.0 60-64 33.3898 38.0 34.0 38.0 16.0 38.0 65-69 32.847500000000004 38.0 33.2 38.0 16.0 38.0 70-74 32.21715 37.8 31.0 38.0 15.0 38.0 75-79 31.792650000000002 37.0 29.8 38.0 15.0 38.0 80-84 31.218799999999998 37.0 29.0 38.0 14.6 38.0 85-89 30.597249999999995 36.2 27.2 38.0 13.6 38.0 90-94 29.95365 36.0 25.6 38.0 13.0 38.0 95-99 29.08265 35.2 23.0 38.0 8.6 38.0 100-104 28.173099999999998 34.2 20.2 38.0 2.0 38.0 105-109 27.18685 34.0 15.0 38.0 2.0 38.0 110-114 26.037599999999998 33.4 14.6 37.8 2.0 38.0 115-119 25.2181 32.6 13.8 37.8 2.0 38.0 120-124 23.98655 30.6 13.0 36.8 2.0 38.0 125-129 22.665399999999998 27.6 4.2 36.0 2.0 38.0 130-134 21.0749 23.4 2.0 35.0 2.0 38.0 135-139 19.3915 20.2 2.0 35.0 2.0 38.0 140-144 17.38295 14.0 2.0 34.0 2.0 38.0 145-149 15.138049999999998 4.2 2.0 33.8 2.0 38.0 150-151 11.560875 2.0 2.0 27.5 2.0 36.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41.0 3 17.0 4 10.0 5 13.0 6 4.0 7 12.0 8 17.0 9 13.0 10 12.0 11 18.0 12 28.0 13 25.0 14 25.0 15 24.0 16 41.0 17 45.0 18 46.0 19 54.0 20 59.0 21 80.0 22 71.0 23 90.0 24 116.0 25 103.0 26 136.0 27 148.0 28 169.0 29 195.0 30 192.0 31 252.0 32 271.0 33 323.0 34 403.0 35 419.0 36 363.0 37 165.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.725 17.65 13.125 29.5 2 29.539769884942473 23.51175587793897 23.06153076538269 23.88694347173587 3 23.861930965482742 24.387193596798397 25.83791895947974 25.912956478239117 4 26.394796097072803 29.572179134350762 20.665499124343256 23.367525644233176 5 28.83941970985493 30.81540770385193 18.734367183591797 21.61080540270135 6 25.70855279658891 33.28317030348633 19.764233759719087 21.24404314020567 7 22.698771005768748 20.993227990970656 32.45548031101079 23.85252069224981 8 23.551542513167796 24.404314020566844 22.74893403561575 29.29520943064961 9 24.028091296714322 23.727113117632307 26.31050915475295 25.93428643090043 10-14 26.385753699523452 25.573112616002007 21.715575620767495 26.32555806370705 15-19 27.04790569350389 25.352395284675193 23.300727363932783 24.298971657888135 20-24 26.576373212942062 25.728618008527715 23.25056433408578 24.444444444444443 25-29 27.163280662151994 25.116629044394283 22.53824931025834 25.181840983195386 30-34 27.19975920537775 25.132938697702418 23.888833149392998 23.778468947526836 35-39 25.891916302875206 24.702694565708263 23.939986953685583 25.465402177730944 40-44 27.291426278031405 24.547233231324938 24.010434957106305 24.15090553353735 45-49 26.385753699523452 24.028091296714322 24.604966139954854 24.981188863807375 50-54 25.71614909948327 23.869964380675263 25.169317212662417 25.24456930717905 55-59 26.37070479056935 24.319036869826938 24.951091045899172 24.35916729370454 60-64 24.238776022071733 25.613243039879606 25.4477050413845 24.70027589666416 65-69 24.790569350388765 26.175068974166038 24.544770504138448 24.489591171306746 70-74 24.411779461195003 25.44022475292229 24.70777103296042 25.44022475292229 75-79 24.1294530858003 26.537882589061716 24.440541896638234 24.89212242849975 80-84 24.836929252383342 26.81384846964375 23.903662819869542 24.44555945810336 85-89 24.36540583926959 26.04595164041336 24.972408949533463 24.616233570783585 90-94 24.957350727546412 26.40240842950326 24.67134972403412 23.968891118916208 95-99 24.754164158137666 27.31788079470199 24.67389123018262 23.254063816977723 100-104 25.603210433910206 26.39578630549285 23.707047905693504 24.293955354903435 105-109 25.609633718013047 27.757150025087807 23.46713497240341 23.166081284495736 110-114 24.714027694160144 28.090507726269315 22.797511539233394 24.397953040337146 115-119 25.647200481637565 28.246036524182223 22.09010636162954 24.016656632550674 120-124 25.4276398294457 27.87057938299473 23.185352395284674 23.516428392274893 125-129 26.101354741595586 28.830908178625187 21.73105870546914 23.336678374310086 130-134 26.42882231923328 27.61804405640022 22.605248632645893 23.34788499172061 135-139 26.059694005517937 28.587910709806874 22.061700526711814 23.290694757963383 140-144 27.389014296463504 27.97592174567344 22.618510158013542 22.01655379984951 145-149 27.007775269626283 28.843742162026587 21.710559317782792 22.437923250564335 150-151 27.45171808377226 30.147980938048658 20.968146476047153 21.43215450213193 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 2.5 2 3.0 3 3.5 4 2.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.5 18 0.5 19 0.0 20 0.5 21 0.5 22 1.0 23 1.0 24 0.5 25 2.5 26 3.0 27 3.5 28 5.5 29 10.0 30 15.0 31 20.5 32 22.0 33 22.0 34 36.0 35 44.0 36 56.0 37 85.5 38 107.5 39 118.0 40 161.0 41 139.0 42 96.0 43 112.0 44 105.0 45 118.5 46 133.0 47 131.5 48 143.5 49 138.0 50 120.0 51 129.5 52 148.0 53 149.0 54 151.0 55 144.5 56 126.5 57 116.0 58 113.0 59 113.0 60 94.0 61 82.0 62 84.0 63 78.5 64 66.0 65 61.5 66 60.0 67 50.5 68 47.0 69 49.0 70 40.5 71 29.0 72 26.0 73 23.0 74 16.5 75 11.5 76 8.5 77 4.0 78 3.0 79 2.5 80 1.5 81 1.5 82 0.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.05 4 0.075 5 0.05 6 0.325 7 0.325 8 0.325 9 0.325 10-14 0.325 15-19 0.325 20-24 0.325 25-29 0.325 30-34 0.33 35-39 0.35500000000000004 40-44 0.335 45-49 0.325 50-54 0.335 55-59 0.325 60-64 0.325 65-69 0.325 70-74 0.335 75-79 0.35000000000000003 80-84 0.35000000000000003 85-89 0.33 90-94 0.35000000000000003 95-99 0.33999999999999997 100-104 0.325 105-109 0.35000000000000003 110-114 0.33999999999999997 115-119 0.33999999999999997 120-124 0.325 125-129 0.35000000000000003 130-134 0.35500000000000004 135-139 0.325 140-144 0.325 145-149 0.325 150-151 0.325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.675 #Duplication Level Percentage of deduplicated Percentage of total 1 92.08739297313257 77.97500000000001 2 5.137289636846766 8.7 3 1.3876586950103336 3.5249999999999995 4 0.41334514319456744 1.4000000000000001 5 0.3247711839385887 1.375 6 0.029524653085326247 0.15 7 0.029524653085326247 0.17500000000000002 8 0.029524653085326247 0.2 9 0.11809861234130499 0.8999999999999999 >10 0.44286979627989376 5.6000000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 24 0.6 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 21 0.525 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 21 0.525 Illumina Single End PCR Primer 1 (100% over 50bp) GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 19 0.475 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 18 0.44999999999999996 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 16 0.4 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 14 0.35000000000000003 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 14 0.35000000000000003 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 14 0.35000000000000003 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 12 0.3 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 11 0.27499999999999997 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 10 0.25 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 10 0.25 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 10 0.25 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 10 0.25 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 9 0.22499999999999998 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 9 0.22499999999999998 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 9 0.22499999999999998 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 9 0.22499999999999998 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 8 0.2 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 7 0.17500000000000002 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 6 0.15 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 5 0.125 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 5 0.125 No Hit CCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATT 5 0.125 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 5 0.125 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 5 0.125 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 5 0.125 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 5 0.125 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 5 0.125 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 5 0.125 No Hit GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT 5 0.125 No Hit CACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.30000000000000004 0.0 0.0 0.0 0.0 84-85 0.3875 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.7625 0.0 0.0 0.0 0.0 92-93 0.9125 0.0 0.0 0.0 0.0 94-95 1.0499999999999998 0.0 0.0 0.0 0.0 96-97 1.225 0.0 0.0 0.0 0.0 98-99 1.4125 0.0 0.0 0.0 0.0 100-101 1.5875 0.0 0.0 0.0 0.0 102-103 1.9 0.0 0.0 0.0 0.0 104-105 2.2625 0.0 0.0 0.0 0.0 106-107 2.675 0.0 0.0 0.0 0.0 108-109 3.125 0.0 0.0 0.0 0.0 110-111 3.35 0.0 0.0 0.0 0.0 112-113 3.75 0.0 0.0 0.0 0.0 114-115 4.275 0.0 0.0 0.0 0.0 116-117 4.6875 0.0 0.0 0.0 0.0 118-119 5.0 0.0 0.0 0.0 0.0 120-121 5.5375 0.0 0.0 0.0 0.0 122-123 6.1625 0.0 0.0 0.0 0.0 124-125 6.6875 0.0 0.0 0.0 0.0 126-127 7.025 0.0 0.0 0.0 0.0 128-129 7.4125 0.0 0.0 0.0 0.0 130-131 7.825 0.0 0.0 0.0 0.0 132-133 8.225 0.0 0.0 0.0 0.0 134-135 8.8125 0.0 0.0 0.0 0.0 136-137 9.4875 0.0 0.0 0.0 0.0 138-139 9.8875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCTC 10 0.006830828 145.0 2 TTGCTCG 10 0.006830828 145.0 3 >>END_MODULE Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799064 spots for SRR5578497.sra Written 799064 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra Read 799052 spots for SRR5578497.sra Written 799052 spots for SRR5578497.sra SRR ids: ['SRR5578497.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1mwj8ji1 SRR5578497.sra spots: 15981052 blocks: [[1, 799052], [799053, 1598104], [1598105, 2397156], [2397157, 3196208], [3196209, 3995260], [3995261, 4794312], [4794313, 5593364], [5593365, 6392416], [6392417, 7191468], [7191469, 7990520], [7990521, 8789572], [8789573, 9588624], [9588625, 10387676], [10387677, 11186728], [11186729, 11985780], [11985781, 12784832], [12784833, 13583884], [13583885, 14382936], [14382937, 15181988], [15181989, 15981052]] SRR5578497 file size 5393753 SRR5578497 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578497 SRR5578497_1.fastq SRR5578497_2.fastq Input file: SRR5578497_1.fastq Paired file: SRR5578497_2.fastq trimmed: SRR5578497-trimmed-pair1.fastq, SRR5578497-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 03:19:14 2024 >> started Thu Dec 12 03:19:33 2024 >> done (18.587s) 15981052 read pairs processed; of these: 58031 ( 0.36%) short read pairs filtered out after trimming by size control 185180 ( 1.16%) empty read pairs filtered out after trimming by size control 15737841 (98.48%) read pairs available; of these: 8827791 (56.09%) trimmed read pairs available after processing 6910050 (43.91%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 9 0.00% 20 10 0.00% 21 18 0.00% 22 23 0.00% 23 27 0.00% 24 24 0.00% 25 35 0.00% 26 15 0.00% 27 29 0.00% 28 27 0.00% 29 26 0.00% 30 28 0.00% 31 32 0.00% 32 45 0.00% 33 36 0.00% 34 37 0.00% 35 43 0.00% 36 31 0.00% 37 42 0.00% 38 48 0.00% 39 53 0.00% 40 64 0.00% 41 68 0.00% 42 64 0.00% 43 84 0.00% 44 128 0.00% 45 138 0.00% 46 159 0.00% 47 163 0.00% 48 199 0.00% 49 213 0.00% 50 252 0.00% 51 280 0.00% 52 306 0.00% 53 319 0.00% 54 339 0.00% 55 433 0.00% 56 454 0.00% 57 528 0.00% 58 588 0.00% 59 561 0.00% 60 751 0.00% 61 778 0.00% 62 928 0.01% 63 957 0.01% 64 1136 0.01% 65 1358 0.01% 66 1677 0.01% 67 2089 0.01% 68 2773 0.02% 69 5116 0.03% 70 5881 0.04% 71 3607 0.02% 72 3347 0.02% 73 3602 0.02% 74 3836 0.02% 75 4440 0.03% 76 4860 0.03% 77 5541 0.04% 78 6012 0.04% 79 6936 0.04% 80 7678 0.05% 81 8550 0.05% 82 9760 0.06% 83 11228 0.07% 84 14067 0.09% 85 16516 0.10% 86 17833 0.11% 87 19423 0.12% 88 20836 0.13% 89 21845 0.14% 90 23236 0.15% 91 23754 0.15% 92 24561 0.16% 93 26223 0.17% 94 27774 0.18% 95 29286 0.19% 96 30652 0.19% 97 32270 0.21% 98 33747 0.21% 99 34952 0.22% 100 37818 0.24% 101 39526 0.25% 102 41179 0.26% 103 43660 0.28% 104 45978 0.29% 105 48118 0.31% 106 50015 0.32% 107 52487 0.33% 108 55534 0.35% 109 55682 0.35% 110 57505 0.37% 111 59466 0.38% 112 62299 0.40% 113 66958 0.43% 114 69866 0.44% 115 73953 0.47% 116 74344 0.47% 117 75757 0.48% 118 76462 0.49% 119 77695 0.49% 120 81474 0.52% 121 82344 0.52% 122 85033 0.54% 123 87139 0.55% 124 91718 0.58% 125 93835 0.60% 126 96154 0.61% 127 98675 0.63% 128 98290 0.62% 129 102711 0.65% 130 102818 0.65% 131 104107 0.66% 132 106695 0.68% 133 109901 0.70% 134 113863 0.72% 135 115827 0.74% 136 117094 0.74% 137 121110 0.77% 138 125714 0.80% 139 131856 0.84% 140 137710 0.88% 141 140765 0.89% 142 154851 0.98% 143 164418 1.04% 144 177063 1.13% 145 199403 1.27% 146 228556 1.45% 147 278248 1.77% 148 373605 2.37% 149 623111 3.96% 150 2613598 16.61% 151 6910050 43.91% 15737841 reads passed initial QC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=2.58 fanout-score-rank=19 prefix-density=0.53 prefix-fanout=2.5 sequence=CCCAGGCGTTGTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=53.20 fanout-score-rank=1 prefix-density=0.42 prefix-fanout=1.3 sequence=TTTTTTTTCTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATTCCCGAGGCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCCCAGAGACGGGCGGAGGCTCTTAACCGTACGAGACAATCTCC criterion=sequence-density sequence-density=0.74 sequence-density-rank=1 fanout-score=4.68 fanout-score-rank=12 prefix-density=3.44 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=48.42 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=3.5 sequence=CAGCAGCGATTAAGGCAGAGGCGTTTGTATCTGCCATTATAAAGAAGTTTCCTCCAGCAACTCCTTTCTTAATTCCAAACTTAGCTTCAGTTATAAATTCCCCTCCCATGATTGGGATTTTATAAACTTTTCTTCCATATAATTCATCTTTCTTCTCATAACCGTCTCCGAAAAACTTCAACTTAAATCCAACCTTTAACTGCTCATCAGCCATGTCTCCCACAGCATCAAAAATAGCAGTTGTTGGACATGTTAAGACACACTGCCCCAATCTCTCTAACATTTGATGCTCTAACTCTGACTTTTTAGGGTGGCATATCTGTATTATAAATCCTGGTCTTCCATCTGGTGTTTTTGATGGAGGGACATATTTCTCAATTCCTGCTTCTGCTGGACACATTATAACTGAACAACCAAAACCTGTTGCCTCTGTAGCTGCAATCTTAGCCCACTTCTTTGTAGCTGCTGTTATTAAAACTCTTGAAACCCATATTGGGAATGCTTCTGCAAA SRR5578497 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 12 03:21:02 Started mapping on | Dec 12 03:21:02 Finished on | Dec 12 03:43:17 Mapping speed, Million of reads per hour | 42.44 Number of input reads | 15737841 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 8813556 Uniquely mapped reads % | 56.00% Average mapped length | 282.86 Number of splices: Total | 5767716 Number of splices: Annotated (sjdb) | 5440351 Number of splices: GT/AG | 5701660 Number of splices: GC/AG | 57637 Number of splices: AT/AC | 3550 Number of splices: Non-canonical | 4869 Mismatch rate per base, % | 0.14% Deletion rate per base | 0.00% Deletion average length | 1.37 Insertion rate per base | 0.00% Insertion average length | 1.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 86929 % of reads mapped to multiple loci | 0.55% Number of reads mapped to too many loci | 15192 % of reads mapped to too many loci | 0.10% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 43.01% % of reads unmapped: other | 0.34% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6853731 6853731 6853731 N_multimapping 86929 86929 86929 N_noFeature 153989 8485716 268830 N_ambiguous 248054 614 36014 UnstrandedReadsAssigned:8411513 PositiveStrandReadsAssigned:327226 NegativeStrandReadsAssigned:8508712 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=141 echo kmer=137 SRR5578497 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578497-trimmed-pair1.fastq SRR5578497-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,737,841 reads, 8,530,673 reads pseudoaligned [quant] estimated average fragment length: 199.667 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,072 rounds 52973 SRR5578497.ke.tsv 35125 SRR5578497.se.tsv 88098 total ==> SRR5578497.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 737.459 15.8036 2.80787 PNS24247 1044 845.333 0 0 PNS24249 1928 1729.33 0 0 PNS24246 1044 845.333 0 0 PNS24248 1044 845.333 0 0 PNS24244 1471 1272.33 45.1964 4.65437 PNS24243 293 117.081 0 0 KQK14069 1603 1404.33 99.6491 9.2974 KQK14071 474 279.474 0 0 ==> SRR5578497.se.tsv <== BRADI_1g14170v3 109 BRADI_1g53295v3 21 BRADI_1g59795v3 38 BRADI_1g07683v3 0 BRADI_1g00485v3 6 BRADI_1g20270v3 231 BRADI_1g74790v3 260 BRADI_1g09890v3 9 BRADI_1g77505v3 131 BRADI_1g48960v3 0 SRR5578497 completed mapping pipeline successfully