Starting /dee2/code/volunteer_pipeline.sh SRR5578504 current disk space = 1521906888704 free memory = 1569968804 SRR5578504 SRAfilesize e82132f039ab2c79c17937d9ec138c23 SRR5578504.sra SRR5578504.sra file validated SRR5578504 is paired end SRR5578504 is conventional basespace SRR5578504 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578504_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.33475 34.0 33.0 34.0 33.0 34.0 2 33.45325 34.0 34.0 34.0 33.0 34.0 3 33.437 34.0 34.0 34.0 33.0 34.0 4 33.42475 34.0 34.0 34.0 33.0 34.0 5 33.39975 34.0 34.0 34.0 33.0 34.0 6 37.17025 38.0 38.0 38.0 36.0 38.0 7 37.331 38.0 38.0 38.0 37.0 38.0 8 37.386 38.0 38.0 38.0 37.0 38.0 9 37.541 38.0 38.0 38.0 38.0 38.0 10-14 37.4431 38.0 38.0 38.0 37.0 38.0 15-19 37.44305 38.0 38.0 38.0 37.2 38.0 20-24 37.3737 38.0 38.0 38.0 37.0 38.0 25-29 37.3309 38.0 38.0 38.0 37.2 38.0 30-34 37.2726 38.0 38.0 38.0 37.0 38.0 35-39 37.173 38.0 38.0 38.0 37.0 38.0 40-44 37.0329 38.0 38.0 38.0 36.6 38.0 45-49 37.08945 38.0 38.0 38.0 37.0 38.0 50-54 37.10145 38.0 38.0 38.0 36.8 38.0 55-59 37.00025 38.0 38.0 38.0 36.4 38.0 60-64 36.957249999999995 38.0 38.0 38.0 36.0 38.0 65-69 36.94435 38.0 38.0 38.0 36.6 38.0 70-74 36.68295 38.0 38.0 38.0 35.8 38.0 75-79 36.0578 38.0 38.0 38.0 34.6 38.0 80-84 35.9424 38.0 38.0 38.0 34.4 38.0 85-89 35.9049 38.0 38.0 38.0 34.0 38.0 90-94 35.7952 38.0 38.0 38.0 34.0 38.0 95-99 35.7207 38.0 38.0 38.0 34.0 38.0 100-104 35.591750000000005 38.0 38.0 38.0 33.2 38.0 105-109 35.46045 38.0 38.0 38.0 33.0 38.0 110-114 35.34125 38.0 38.0 38.0 32.6 38.0 115-119 35.18455 38.0 38.0 38.0 31.0 38.0 120-124 35.2106 38.0 37.8 38.0 32.0 38.0 125-129 34.97855 38.0 37.0 38.0 30.6 38.0 130-134 34.8457 38.0 36.4 38.0 29.2 38.0 135-139 34.6314 38.0 36.0 38.0 27.8 38.0 140-144 34.353500000000004 38.0 36.0 38.0 26.2 38.0 145-149 33.884249999999994 38.0 35.4 38.0 22.8 38.0 150-151 30.959875 36.5 30.0 38.0 8.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 0.0 5 1.0 6 6.0 7 2.0 8 4.0 9 3.0 10 2.0 11 6.0 12 2.0 13 1.0 14 2.0 15 4.0 16 3.0 17 13.0 18 37.0 19 52.0 20 9.0 21 5.0 22 11.0 23 3.0 24 12.0 25 10.0 26 16.0 27 8.0 28 15.0 29 32.0 30 34.0 31 39.0 32 52.0 33 61.0 34 98.0 35 157.0 36 394.0 37 2903.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.14895754835469 11.906556141672947 10.499874403416227 30.44461190655614 2 28.050000000000004 13.575000000000001 29.575000000000003 28.799999999999997 3 24.975 14.649999999999999 26.200000000000003 34.175 4 27.175 22.900000000000002 22.85 27.075 5 27.250000000000004 25.05 27.55 20.150000000000002 6 24.075 30.4 26.85 18.675 7 14.875 25.4 44.925 14.799999999999999 8 19.35 27.900000000000002 32.975 19.775000000000002 9 20.45 24.65 36.225 18.675 10-14 23.465 27.35 27.97 21.215 15-19 22.05 26.900000000000002 28.455000000000002 22.595000000000002 20-24 22.78 25.805 28.139999999999997 23.275000000000002 25-29 20.7 27.98 29.759999999999998 21.560000000000002 30-34 20.225 28.165000000000003 28.515 23.095 35-39 21.584999999999997 27.58 28.425 22.41 40-44 23.1 26.935 27.36 22.605 45-49 23.494999999999997 26.795 29.635 20.075000000000003 50-54 24.985 26.090000000000003 26.965 21.959999999999997 55-59 22.725 26.31 28.955 22.009999999999998 60-64 20.76 27.860000000000003 28.95 22.43 65-69 20.46 30.495 27.3 21.745 70-74 22.155 28.955 26.695 22.195 75-79 21.59 27.625 27.11 23.674999999999997 80-84 22.605 28.33 27.025 22.040000000000003 85-89 22.935 27.345000000000002 27.6 22.12 90-94 22.975 26.255 28.74 22.03 95-99 22.07 25.835 28.835 23.26 100-104 22.98 27.37 27.38 22.27 105-109 22.115000000000002 28.015 28.425 21.445 110-114 21.425 27.485 26.715 24.375 115-119 21.39 28.505000000000003 26.56 23.544999999999998 120-124 22.665 28.335 25.540000000000003 23.46 125-129 22.105 28.645 25.545 23.705000000000002 130-134 22.32 27.465 26.26 23.955000000000002 135-139 21.54 29.095 27.355 22.009999999999998 140-144 22.935 28.035 26.575 22.455 145-149 22.375 29.875 24.465 23.285 150-151 22.3375 27.525 25.5375 24.6 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 1.5 6 2.5 7 2.5 8 2.0 9 1.5 10 1.0 11 0.0 12 1.0 13 2.0 14 1.5 15 0.5 16 0.0 17 1.0 18 2.0 19 1.0 20 1.5 21 2.5 22 2.5 23 2.0 24 1.0 25 1.0 26 2.5 27 3.5 28 6.5 29 26.0 30 40.5 31 55.5 32 64.0 33 73.0 34 84.0 35 90.0 36 119.0 37 181.5 38 196.0 39 143.5 40 142.0 41 139.5 42 107.5 43 100.0 44 117.5 45 145.5 46 167.5 47 180.5 48 202.0 49 207.0 50 178.5 51 161.5 52 164.0 53 167.0 54 152.5 55 120.5 56 92.5 57 74.0 58 56.0 59 54.5 60 46.0 61 25.0 62 15.5 63 17.0 64 20.0 65 11.0 66 4.5 67 2.5 68 0.5 69 0.0 70 1.0 71 1.5 72 1.5 73 1.5 74 1.5 75 1.5 76 1.0 77 0.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.475 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.925 #Duplication Level Percentage of deduplicated Percentage of total 1 75.09232663110382 45.75 2 12.556421830119 15.299999999999999 3 5.416495691423882 9.9 4 2.5030775543701274 6.1 5 1.7234304472712352 5.25 6 0.8206811653672548 3.0 7 0.5744768157570783 2.45 8 0.2051702913418137 1.0 9 0.28723840787853916 1.575 >10 0.7796471070988921 7.249999999999999 >50 0.04103405826836274 2.4250000000000003 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAAATCTCGTATGC 97 2.4250000000000003 TruSeq Adapter, Index 2 (97% over 36bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 43 1.075 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 26 0.65 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 21 0.525 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 17 0.42500000000000004 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 15 0.375 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 15 0.375 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 14 0.35000000000000003 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 14 0.35000000000000003 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 13 0.325 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 13 0.325 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 12 0.3 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 12 0.3 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 11 0.27499999999999997 No Hit GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA 11 0.27499999999999997 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 11 0.27499999999999997 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 11 0.27499999999999997 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 11 0.27499999999999997 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 10 0.25 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 10 0.25 No Hit GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA 9 0.22499999999999998 No Hit GTCCAAAGTTAAGCTCTGCACCTGTTACACTATAGTCATCGTCCATAACA 9 0.22499999999999998 No Hit GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG 9 0.22499999999999998 No Hit CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT 9 0.22499999999999998 No Hit TGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATAT 9 0.22499999999999998 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 9 0.22499999999999998 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 9 0.22499999999999998 No Hit GCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGAC 8 0.2 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 8 0.2 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 8 0.2 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 8 0.2 No Hit GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG 8 0.2 No Hit CAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCA 7 0.17500000000000002 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 7 0.17500000000000002 No Hit GGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATA 7 0.17500000000000002 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 7 0.17500000000000002 No Hit GTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTG 7 0.17500000000000002 No Hit ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT 7 0.17500000000000002 No Hit GGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTT 7 0.17500000000000002 No Hit GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA 7 0.17500000000000002 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 7 0.17500000000000002 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 7 0.17500000000000002 No Hit CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAA 7 0.17500000000000002 No Hit GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT 7 0.17500000000000002 No Hit CGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAA 7 0.17500000000000002 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 7 0.17500000000000002 No Hit GCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTC 6 0.15 No Hit CCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTT 6 0.15 No Hit GTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGG 6 0.15 No Hit GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT 6 0.15 No Hit CCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAA 6 0.15 No Hit CCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGC 6 0.15 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 6 0.15 No Hit GCAGGGATATTATTTTATTGCATTGTATTTCATCTTACCCAACCCCTTAT 6 0.15 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 6 0.15 No Hit GTCAATTTAAACGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGT 6 0.15 No Hit CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA 6 0.15 No Hit GGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG 6 0.15 No Hit CTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGG 6 0.15 No Hit CGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTG 6 0.15 No Hit TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT 6 0.15 No Hit GCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAG 6 0.15 No Hit ACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAAC 6 0.15 No Hit GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC 6 0.15 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 6 0.15 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 6 0.15 No Hit CAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTT 5 0.125 No Hit CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT 5 0.125 No Hit CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA 5 0.125 No Hit GCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTG 5 0.125 No Hit GGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTG 5 0.125 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 5 0.125 No Hit GGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATT 5 0.125 No Hit GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT 5 0.125 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 5 0.125 No Hit GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA 5 0.125 No Hit CTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACA 5 0.125 No Hit GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC 5 0.125 No Hit CTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGC 5 0.125 No Hit GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT 5 0.125 No Hit GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA 5 0.125 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5 0.125 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 5 0.125 No Hit CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC 5 0.125 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 5 0.125 No Hit CCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGG 5 0.125 No Hit GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG 5 0.125 No Hit GGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC 5 0.125 No Hit CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA 5 0.125 No Hit ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT 5 0.125 No Hit GGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCA 5 0.125 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 5 0.125 No Hit GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT 5 0.125 No Hit CCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCA 5 0.125 No Hit TGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTG 5 0.125 No Hit GGTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAA 5 0.125 No Hit CACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCC 5 0.125 No Hit GCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATG 5 0.125 No Hit GCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAAT 5 0.125 No Hit CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT 5 0.125 No Hit GACCATACATTGGGAATACTCGCCCCAGTAGTTTCTGTTGCCTTAGGAGC 5 0.125 No Hit GGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAG 5 0.125 No Hit CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA 5 0.125 No Hit GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA 5 0.125 No Hit CCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATT 5 0.125 No Hit TCTCAAAGAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTAC 5 0.125 No Hit CCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGAC 5 0.125 No Hit GTGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.30000000000000004 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.7125 0.0 0.0 0.0 0.0 92-93 0.8875 0.0 0.0 0.0 0.0 94-95 1.2125 0.0 0.0 0.0 0.0 96-97 1.4874999999999998 0.0 0.0 0.0 0.0 98-99 1.8250000000000002 0.0 0.0 0.0 0.0 100-101 2.1375 0.0 0.0 0.0 0.0 102-103 2.5625 0.0 0.0 0.0 0.0 104-105 2.9125 0.0 0.0 0.0 0.0 106-107 3.375 0.0 0.0 0.0 0.0 108-109 3.7874999999999996 0.0 0.0 0.0 0.0 110-111 4.3125 0.0 0.0 0.0 0.0 112-113 4.7875 0.0 0.0 0.0 0.0 114-115 5.4625 0.0 0.0 0.0 0.0 116-117 6.2125 0.0 0.0 0.0 0.0 118-119 6.9 0.0 0.0 0.0 0.0 120-121 7.6 0.0 0.0 0.0 0.0 122-123 8.1375 0.0 0.0 0.0 0.0 124-125 8.662500000000001 0.0 0.0 0.0 0.0 126-127 9.35 0.0 0.0 0.0 0.0 128-129 9.8625 0.0 0.0 0.0 0.0 130-131 10.65 0.0 0.0 0.0 0.0 132-133 11.462499999999999 0.0 0.0 0.0 0.0 134-135 12.212499999999999 0.0 0.0 0.0 0.0 136-137 12.9625 0.0 0.0 0.0 0.0 138-139 13.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTTTT 220 0.009102302 6.5909095 6 >>END_MODULE SRR5578504 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578504_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.3105 33.0 31.0 33.0 18.0 34.0 2 30.5305 33.0 31.0 33.0 18.0 34.0 3 30.38625 33.0 30.0 33.0 18.0 34.0 4 30.42025 33.0 31.0 33.0 25.0 34.0 5 30.351 33.0 31.0 33.0 15.0 34.0 6 34.12075 38.0 34.0 38.0 26.0 38.0 7 34.09475 38.0 34.0 38.0 26.0 38.0 8 34.0645 38.0 34.0 38.0 16.0 38.0 9 34.06 38.0 34.0 38.0 16.0 38.0 10-14 33.8836 38.0 34.0 38.0 16.0 38.0 15-19 33.681400000000004 38.0 34.0 38.0 17.8 38.0 20-24 33.311600000000006 38.0 33.6 38.0 16.0 38.0 25-29 32.96255 37.8 33.0 38.0 16.0 38.0 30-34 32.317550000000004 37.0 30.6 38.0 16.0 38.0 35-39 31.86205 37.0 29.2 38.0 16.0 38.0 40-44 31.7959 37.0 29.0 38.0 16.0 38.0 45-49 31.45045 37.0 29.0 38.0 16.0 38.0 50-54 31.070549999999997 36.2 28.2 38.0 15.6 38.0 55-59 30.738649999999996 36.0 27.8 38.0 15.0 38.0 60-64 30.428350000000002 36.0 27.0 38.0 15.0 38.0 65-69 29.78675 35.4 26.2 38.0 14.6 38.0 70-74 29.100450000000002 35.0 24.4 38.0 14.0 38.0 75-79 28.3898 34.0 17.6 38.0 13.4 38.0 80-84 27.611849999999997 34.0 15.6 38.0 2.0 38.0 85-89 26.633099999999995 33.2 15.0 37.0 2.0 38.0 90-94 25.663400000000003 31.2 15.0 37.0 2.0 38.0 95-99 24.777749999999997 29.6 15.0 37.0 2.0 38.0 100-104 23.39805 27.6 13.6 36.2 2.0 38.0 105-109 22.2551 26.0 13.0 35.2 2.0 38.0 110-114 20.90745 23.2 2.0 34.6 2.0 38.0 115-119 19.6935 20.2 2.0 34.0 2.0 38.0 120-124 17.947449999999996 15.0 2.0 34.0 2.0 37.4 125-129 16.48375 14.4 2.0 33.0 2.0 37.0 130-134 14.766 11.0 2.0 29.8 2.0 36.2 135-139 13.293599999999998 2.0 2.0 26.6 2.0 35.4 140-144 11.31135 2.0 2.0 22.2 2.0 34.6 145-149 8.9817 2.0 2.0 9.4 2.0 33.4 150-151 6.264875 2.0 2.0 2.0 2.0 26.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60.0 3 24.0 4 23.0 5 28.0 6 21.0 7 26.0 8 20.0 9 22.0 10 23.0 11 32.0 12 36.0 13 51.0 14 51.0 15 63.0 16 61.0 17 84.0 18 68.0 19 105.0 20 110.0 21 139.0 22 132.0 23 142.0 24 186.0 25 197.0 26 208.0 27 216.0 28 194.0 29 243.0 30 268.0 31 306.0 32 272.0 33 258.0 34 189.0 35 92.0 36 45.0 37 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.824999999999996 21.125 14.174999999999999 26.875 2 28.09917355371901 27.848735286751815 24.36764337590784 19.684447783621337 3 22.400400902029567 26.13380105236783 30.719118015534953 20.74668003006765 4 24.93734335839599 30.751879699248118 22.706766917293233 21.604010025062657 5 27.11779448621554 33.40852130325814 21.904761904761905 17.56892230576441 6 24.030037546933666 34.968710888610765 21.501877346683354 19.499374217772214 7 18.883045329326322 26.270974204858504 34.63561232156274 20.210368144252442 8 21.036814425244177 29.526671675432002 24.292511895817682 25.144002003506138 9 24.67434869739479 25.150300601202403 26.528056112224448 23.647294589178355 10-14 24.543996792944476 28.282220885949087 23.421527360192425 23.75225496091401 15-19 24.521303258145362 28.12531328320802 25.047619047619047 22.305764411027567 20-24 24.578905153398836 28.67956687387207 24.23801884900742 22.503509123721678 25-29 25.769577860222604 28.953173568635314 23.588689461546174 21.68855910959591 30-34 25.669307129248974 27.554396871553195 24.987466158628298 21.788829840569537 35-39 24.33204671913379 27.329690711313848 25.20427089077147 23.13399167878089 40-44 25.710419485791608 27.88553099784494 24.90352327970731 21.500526236656142 45-49 24.195488721804512 26.31578947368421 26.516290726817044 22.972431077694235 50-54 23.107769423558896 26.917293233082706 27.42857142857143 22.546365914786968 55-59 21.60902255639098 28.050125313283207 28.411027568922304 21.929824561403507 60-64 20.933380119304225 29.795979748358313 27.03894932076796 22.2316908115695 65-69 20.984559855624624 30.860236615199522 26.218167234810508 21.93703629436535 70-74 21.32330827067669 29.87468671679198 25.45864661654135 23.343358395989974 75-79 21.064768397834367 30.714858632444354 25.020052135552433 23.20032083416884 80-84 21.480850210547423 30.108281531983156 25.496290354922802 22.914577902546622 85-89 20.760014037198577 30.836717300847244 26.37990675289517 22.023361909059005 90-94 22.086320116296555 31.249686701087775 25.830868715223822 20.83312446739185 95-99 21.418546365914786 31.49874686716792 25.86967418546366 21.213032581453632 100-104 22.275689223057643 32.1203007518797 23.859649122807017 21.74436090225564 105-109 22.295197031986362 32.858718540058156 23.032186904642536 21.813897523312946 110-114 21.091948260302818 32.23202647147298 23.729068484909256 22.94695678331495 115-119 21.979949874686717 33.523809523809526 22.69172932330827 21.804511278195488 120-124 22.069000100290843 33.452010831411094 22.831210510480393 21.64777855781767 125-129 21.681371566071785 33.59735311810708 22.493483055945457 22.227792259875674 130-134 21.460798074994987 32.40926408662523 24.273110086224182 21.856827752155606 135-139 22.108007822293537 32.958932958932955 23.49696635410921 21.436092864664293 140-144 23.130138359735312 33.41187086424704 22.623821937036297 20.834168838981352 145-149 22.755751591398926 33.16625733045962 22.795849832088617 21.28214124605283 150-151 21.56371382032327 34.30647788497682 22.691392056133317 21.438416238566596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 3.5 2 2.5 3 0.0 4 0.0 5 1.0 6 1.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 0.5 13 1.5 14 1.0 15 0.0 16 1.0 17 2.5 18 3.0 19 2.5 20 2.0 21 3.5 22 4.5 23 4.5 24 2.5 25 1.0 26 3.5 27 6.5 28 13.5 29 14.5 30 12.0 31 28.0 32 47.0 33 54.5 34 66.5 35 90.0 36 116.5 37 147.5 38 180.5 39 200.0 40 224.0 41 183.0 42 112.5 43 111.0 44 116.5 45 111.5 46 128.5 47 161.5 48 182.0 49 191.0 50 171.5 51 152.0 52 157.0 53 160.5 54 160.5 55 151.0 56 129.0 57 95.5 58 71.0 59 61.0 60 41.5 61 22.5 62 16.5 63 14.5 64 13.5 65 8.0 66 3.0 67 4.0 68 4.0 69 3.5 70 3.5 71 3.0 72 2.5 73 1.0 74 1.5 75 2.5 76 2.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.17500000000000002 3 0.22499999999999998 4 0.25 5 0.25 6 0.125 7 0.17500000000000002 8 0.17500000000000002 9 0.2 10-14 0.22 15-19 0.25 20-24 0.26 25-29 0.27 30-34 0.27 35-39 0.255 40-44 0.23500000000000001 45-49 0.25 50-54 0.25 55-59 0.25 60-64 0.255 65-69 0.26 70-74 0.25 75-79 0.26 80-84 0.26 85-89 0.265 90-94 0.255 95-99 0.25 100-104 0.25 105-109 0.27 110-114 0.27 115-119 0.25 120-124 0.29 125-129 0.26 130-134 0.26 135-139 0.28500000000000003 140-144 0.26 145-149 0.245 150-151 0.2375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.025 #Duplication Level Percentage of deduplicated Percentage of total 1 81.12644535621037 54.37499999999999 2 10.481163744871317 14.05 3 3.729951510630362 7.5 4 1.9768743006340919 5.3 5 0.7459903021260723 2.5 6 0.4475941812756434 1.7999999999999998 7 0.07459903021260723 0.35000000000000003 8 0.3356956359567326 1.7999999999999998 9 0.14919806042521447 0.8999999999999999 >10 0.9324878776575904 11.425 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 36 0.8999999999999999 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 32 0.8 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 31 0.775 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 31 0.775 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 29 0.7250000000000001 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 28 0.7000000000000001 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 27 0.675 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 22 0.5499999999999999 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 19 0.475 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 17 0.42500000000000004 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 16 0.4 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 16 0.4 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 16 0.4 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 15 0.375 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 12 0.3 No Hit CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC 12 0.3 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 12 0.3 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 12 0.3 Illumina Single End PCR Primer 1 (100% over 50bp) CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 11 0.27499999999999997 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 11 0.27499999999999997 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 11 0.27499999999999997 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 11 0.27499999999999997 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 10 0.25 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 10 0.25 No Hit GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA 10 0.25 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 9 0.22499999999999998 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 9 0.22499999999999998 No Hit TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA 9 0.22499999999999998 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 9 0.22499999999999998 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 8 0.2 No Hit AAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTA 8 0.2 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 8 0.2 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 8 0.2 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 8 0.2 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 8 0.2 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 8 0.2 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 8 0.2 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 8 0.2 No Hit CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTG 7 0.17500000000000002 No Hit CGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTT 7 0.17500000000000002 No Hit CTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAA 6 0.15 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 6 0.15 No Hit GCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTT 6 0.15 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 6 0.15 No Hit GGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGG 6 0.15 No Hit GTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAA 6 0.15 No Hit CTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGTT 6 0.15 No Hit CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCC 6 0.15 No Hit GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA 6 0.15 No Hit GCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTAT 6 0.15 No Hit CTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATC 6 0.15 No Hit GTCATTGGCAACAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGA 6 0.15 No Hit TAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACA 5 0.125 No Hit GCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAG 5 0.125 No Hit GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG 5 0.125 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 5 0.125 No Hit GAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGT 5 0.125 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 5 0.125 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 5 0.125 No Hit CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC 5 0.125 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 5 0.125 No Hit ATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGG 5 0.125 No Hit ATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTA 5 0.125 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 5 0.125 No Hit CATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGAT 5 0.125 No Hit GCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACA 5 0.125 No Hit GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG 5 0.125 No Hit CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC 5 0.125 No Hit CGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAAC 5 0.125 No Hit TTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGG 5 0.125 No Hit AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC 5 0.125 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.175 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.2875 0.0 0.0 0.0 0.0 92-93 0.3375 0.0 0.0 0.0 0.0 94-95 0.4625 0.0 0.0 0.0 0.0 96-97 0.575 0.0 0.0 0.0 0.0 98-99 0.725 0.0 0.0 0.0 0.0 100-101 0.8625 0.0 0.0 0.0 0.0 102-103 0.9874999999999999 0.0 0.0 0.0 0.0 104-105 1.0875 0.0 0.0 0.0 0.0 106-107 1.15 0.0 0.0 0.0 0.0 108-109 1.325 0.0 0.0 0.0 0.0 110-111 1.5375 0.0 0.0 0.0 0.0 112-113 1.75 0.0 0.0 0.0 0.0 114-115 1.925 0.0 0.0 0.0 0.0 116-117 2.0625 0.0 0.0 0.0 0.0 118-119 2.175 0.0 0.0 0.0 0.0 120-121 2.4000000000000004 0.0 0.0 0.0 0.0 122-123 2.5125 0.0 0.0 0.0 0.0 124-125 2.6125 0.0 0.0 0.0 0.0 126-127 2.825 0.0 0.0 0.0 0.0 128-129 2.9 0.0 0.0 0.0 0.0 130-131 3.05 0.0 0.0 0.0 0.0 132-133 3.3 0.0 0.0 0.0 0.0 134-135 3.425 0.0 0.0 0.0 0.0 136-137 3.5374999999999996 0.0 0.0 0.0 0.0 138-139 3.6500000000000004 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGTAT 10 0.006830828 145.0 8 CAGTATA 10 0.006830828 145.0 9 CTGCTCA 10 0.006830828 145.0 2 CTCACAG 10 0.006830828 145.0 5 TCACAGT 10 0.006830828 145.0 6 CACAGTA 10 0.006830828 145.0 7 >>END_MODULE Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra Read 996534 spots for SRR5578504.sra Written 996534 spots for SRR5578504.sra Read 996517 spots for SRR5578504.sra Written 996517 spots for SRR5578504.sra SRR ids: ['SRR5578504.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4l7k5efp SRR5578504.sra spots: 19930357 blocks: [[1, 996517], [996518, 1993034], [1993035, 2989551], [2989552, 3986068], [3986069, 4982585], [4982586, 5979102], [5979103, 6975619], [6975620, 7972136], [7972137, 8968653], [8968654, 9965170], [9965171, 10961687], [10961688, 11958204], [11958205, 12954721], [12954722, 13951238], [13951239, 14947755], [14947756, 15944272], [15944273, 16940789], [16940790, 17937306], [17937307, 18933823], [18933824, 19930357]] SRR5578504 file size 6732043 SRR5578504 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578504 SRR5578504_1.fastq SRR5578504_2.fastq Input file: SRR5578504_1.fastq Paired file: SRR5578504_2.fastq trimmed: SRR5578504-trimmed-pair1.fastq, SRR5578504-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 21:19:16 2024 >> started Mon Dec 9 21:19:43 2024 >> done (27.144s) 19930357 read pairs processed; of these: 153981 ( 0.77%) short read pairs filtered out after trimming by size control 635857 ( 3.19%) empty read pairs filtered out after trimming by size control 19140519 (96.04%) read pairs available; of these: 14990264 (78.32%) trimmed read pairs available after processing 4150255 (21.68%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 25 0.00% 19 27 0.00% 20 56 0.00% 21 61 0.00% 22 84 0.00% 23 97 0.00% 24 162 0.00% 25 124 0.00% 26 148 0.00% 27 134 0.00% 28 145 0.00% 29 160 0.00% 30 184 0.00% 31 273 0.00% 32 146 0.00% 33 123 0.00% 34 149 0.00% 35 181 0.00% 36 181 0.00% 37 202 0.00% 38 192 0.00% 39 208 0.00% 40 272 0.00% 41 308 0.00% 42 332 0.00% 43 389 0.00% 44 546 0.00% 45 707 0.00% 46 909 0.00% 47 1038 0.01% 48 1354 0.01% 49 1761 0.01% 50 1828 0.01% 51 1643 0.01% 52 1963 0.01% 53 2001 0.01% 54 1877 0.01% 55 1918 0.01% 56 2023 0.01% 57 2051 0.01% 58 2145 0.01% 59 2256 0.01% 60 2243 0.01% 61 2510 0.01% 62 2744 0.01% 63 3088 0.02% 64 3739 0.02% 65 4428 0.02% 66 5562 0.03% 67 7456 0.04% 68 9764 0.05% 69 16051 0.08% 70 22495 0.12% 71 19942 0.10% 72 14670 0.08% 73 11592 0.06% 74 10484 0.05% 75 10539 0.06% 76 11124 0.06% 77 11835 0.06% 78 12807 0.07% 79 14246 0.07% 80 15232 0.08% 81 16389 0.09% 82 19026 0.10% 83 21402 0.11% 84 29906 0.16% 85 35316 0.18% 86 36235 0.19% 87 37546 0.20% 88 38535 0.20% 89 40131 0.21% 90 40557 0.21% 91 41659 0.22% 92 43405 0.23% 93 45491 0.24% 94 46834 0.24% 95 49520 0.26% 96 52638 0.28% 97 54393 0.28% 98 56228 0.29% 99 58965 0.31% 100 63002 0.33% 101 65460 0.34% 102 67056 0.35% 103 70660 0.37% 104 74083 0.39% 105 77766 0.41% 106 81719 0.43% 107 84143 0.44% 108 88100 0.46% 109 87092 0.46% 110 92377 0.48% 111 96582 0.50% 112 102242 0.53% 113 111883 0.58% 114 116806 0.61% 115 122983 0.64% 116 123830 0.65% 117 128055 0.67% 118 131054 0.68% 119 133816 0.70% 120 142630 0.75% 121 147800 0.77% 122 158078 0.83% 123 166321 0.87% 124 173477 0.91% 125 178985 0.94% 126 183032 0.96% 127 186509 0.97% 128 190066 0.99% 129 196227 1.03% 130 201551 1.05% 131 207220 1.08% 132 212366 1.11% 133 217902 1.14% 134 224166 1.17% 135 235747 1.23% 136 242371 1.27% 137 250648 1.31% 138 263834 1.38% 139 280578 1.47% 140 299684 1.57% 141 311332 1.63% 142 349425 1.83% 143 384555 2.01% 144 428124 2.24% 145 506289 2.65% 146 536233 2.80% 147 651276 3.40% 148 829440 4.33% 149 1177714 6.15% 150 2530865 13.22% 151 4150255 21.68% 19140519 reads passed initial QC criterion=sequence-density sequence-density=0.63 sequence-density-rank=1 fanout-score=21.68 fanout-score-rank=6 prefix-density=7.07 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.08 sequence-density-rank=15 fanout-score=52.28 fanout-score-rank=1 prefix-density=4.20 prefix-fanout=1.0 sequence=ATTTCGTTTTTTCTGAGA criterion=sequence-density sequence-density=0.81 sequence-density-rank=1 fanout-score=4.92 fanout-score-rank=31 prefix-density=3.74 prefix-fanout=1.1 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.08 sequence-density-rank=31 fanout-score=154.37 fanout-score-rank=1 prefix-density=12.50 prefix-fanout=1.0 sequence=TCACCATCATGATATTAATGATA SRR5578504 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 21:22:04 Started mapping on | Dec 09 21:22:04 Finished on | Dec 09 22:13:07 Mapping speed, Million of reads per hour | 22.50 Number of input reads | 19140519 Average input read length | 275 UNIQUE READS: Uniquely mapped reads number | 986032 Uniquely mapped reads % | 5.15% Average mapped length | 269.77 Number of splices: Total | 629154 Number of splices: Annotated (sjdb) | 588354 Number of splices: GT/AG | 621015 Number of splices: GC/AG | 7005 Number of splices: AT/AC | 461 Number of splices: Non-canonical | 673 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.00% Deletion average length | 1.42 Insertion rate per base | 0.00% Insertion average length | 1.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 53145 % of reads mapped to multiple loci | 0.28% Number of reads mapped to too many loci | 43345 % of reads mapped to too many loci | 0.23% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 93.24% % of reads unmapped: other | 1.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 18108139 18108139 18108139 N_multimapping 53145 53145 53145 N_noFeature 74621 950767 86831 N_ambiguous 26922 1659 4396 UnstrandedReadsAssigned:884489 PositiveStrandReadsAssigned:33606 NegativeStrandReadsAssigned:894805 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=143 echo kmer=139 SRR5578504 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578504-trimmed-pair1.fastq SRR5578504-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,140,519 reads, 919,683 reads pseudoaligned [quant] estimated average fragment length: 202.316 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,060 rounds 52973 SRR5578504.ke.tsv 35125 SRR5578504.se.tsv 88098 total ==> SRR5578504.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 734.805 0 0 PNS24247 1044 842.684 0 0 PNS24249 1928 1726.68 0 0 PNS24246 1044 842.684 0 0 PNS24248 1044 842.684 0 0 PNS24244 1471 1269.68 16 15.5181 PNS24243 293 116.874 0 0 KQK14069 1603 1401.68 1026.24 901.593 KQK14071 474 277.602 3.7635 16.6948 ==> SRR5578504.se.tsv <== BRADI_1g14170v3 1014 BRADI_1g53295v3 4 BRADI_1g59795v3 6 BRADI_1g07683v3 0 BRADI_1g00485v3 7 BRADI_1g20270v3 81 BRADI_1g74790v3 7 BRADI_1g09890v3 0 BRADI_1g77505v3 26 BRADI_1g48960v3 0 SRR5578504 completed mapping pipeline successfully