Starting /dee2/code/volunteer_pipeline.sh SRR5578505 current disk space = 1521933176832 free memory = 1569964676 SRR5578505 SRAfilesize 6b8e47cc8c782065e3a25ff6f98c129a SRR5578505.sra SRR5578505.sra file validated SRR5578505 is paired end SRR5578505 is conventional basespace SRR5578505 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578505_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.258 34.0 33.0 34.0 33.0 34.0 2 33.36 34.0 33.0 34.0 33.0 34.0 3 33.3915 34.0 33.0 34.0 33.0 34.0 4 33.40375 34.0 34.0 34.0 33.0 34.0 5 33.39525 34.0 33.0 34.0 33.0 34.0 6 37.094 38.0 38.0 38.0 36.0 38.0 7 37.28225 38.0 38.0 38.0 37.0 38.0 8 37.32 38.0 38.0 38.0 37.0 38.0 9 37.43975 38.0 38.0 38.0 37.0 38.0 10-14 37.37905 38.0 38.0 38.0 37.0 38.0 15-19 37.368900000000004 38.0 38.0 38.0 37.0 38.0 20-24 37.3458 38.0 38.0 38.0 37.0 38.0 25-29 37.26235 38.0 38.0 38.0 37.0 38.0 30-34 37.182050000000004 38.0 38.0 38.0 37.0 38.0 35-39 37.15755 38.0 38.0 38.0 37.0 38.0 40-44 36.85245 38.0 38.0 38.0 35.8 38.0 45-49 37.0313 38.0 38.0 38.0 36.4 38.0 50-54 36.99235 38.0 38.0 38.0 36.0 38.0 55-59 36.98805 38.0 38.0 38.0 36.0 38.0 60-64 36.926199999999994 38.0 38.0 38.0 36.0 38.0 65-69 36.83125 38.0 38.0 38.0 35.8 38.0 70-74 36.5219 38.0 38.0 38.0 35.6 38.0 75-79 35.73195 38.0 38.0 38.0 34.0 38.0 80-84 35.6146 38.0 38.0 38.0 33.6 38.0 85-89 35.5437 38.0 38.0 38.0 33.4 38.0 90-94 35.44015 38.0 38.0 38.0 32.8 38.0 95-99 35.3745 38.0 38.0 38.0 32.4 38.0 100-104 35.23405 38.0 38.0 38.0 31.6 38.0 105-109 35.13755 38.0 38.0 38.0 31.0 38.0 110-114 34.9904 38.0 38.0 38.0 29.8 38.0 115-119 34.896550000000005 38.0 38.0 38.0 29.4 38.0 120-124 34.9041 38.0 37.6 38.0 30.6 38.0 125-129 34.69995 38.0 36.8 38.0 28.0 38.0 130-134 34.490300000000005 38.0 36.0 38.0 27.2 38.0 135-139 34.223400000000005 38.0 36.0 38.0 24.2 38.0 140-144 33.98715 38.0 36.0 38.0 22.8 38.0 145-149 33.47665 38.0 35.2 38.0 16.8 38.0 150-151 30.313375 36.5 29.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 1.0 6 6.0 7 4.0 8 4.0 9 1.0 10 2.0 11 3.0 12 1.0 13 1.0 14 4.0 15 10.0 16 11.0 17 6.0 18 64.0 19 45.0 20 10.0 21 9.0 22 3.0 23 9.0 24 11.0 25 12.0 26 15.0 27 32.0 28 22.0 29 40.0 30 37.0 31 42.0 32 59.0 33 68.0 34 75.0 35 173.0 36 399.0 37 2820.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.90253705099221 11.228334589299171 10.324039186134137 30.545089173574482 2 26.474999999999998 15.575 28.7 29.25 3 22.900000000000002 16.75 27.400000000000002 32.95 4 24.7 23.799999999999997 23.075000000000003 28.425 5 27.775 26.075 25.8 20.349999999999998 6 24.425 31.55 24.7 19.325 7 16.650000000000002 27.025 38.75 17.575 8 18.325 27.900000000000002 31.4 22.375 9 22.5 23.474999999999998 33.925 20.1 10-14 22.13 28.925 26.005 22.939999999999998 15-19 21.845 26.615 27.255000000000003 24.285 20-24 21.75 26.974999999999998 27.575 23.7 25-29 21.505 27.474999999999998 27.675 23.345 30-34 21.27 27.810000000000002 26.705000000000002 24.215 35-39 22.025 26.090000000000003 27.345000000000002 24.54 40-44 23.085 26.555 26.384999999999998 23.974999999999998 45-49 23.400000000000002 27.125 27.365000000000002 22.11 50-54 24.675 25.669999999999998 25.835 23.82 55-59 22.3 26.47 27.55 23.68 60-64 21.634999999999998 27.88 26.405 24.08 65-69 21.17 30.165 25.669999999999998 22.994999999999997 70-74 22.17 29.415000000000003 25.064999999999998 23.35 75-79 22.400000000000002 26.915 26.075 24.610000000000003 80-84 22.955000000000002 27.735 25.624999999999996 23.685000000000002 85-89 23.974999999999998 26.915 25.674999999999997 23.435 90-94 23.189999999999998 26.279999999999998 26.490000000000002 24.04 95-99 21.745 26.655 26.935 24.665 100-104 23.305 27.250000000000004 25.585 23.86 105-109 22.42 28.799999999999997 25.16 23.62 110-114 22.125 27.615000000000002 24.925 25.335 115-119 21.921096054802742 28.16640832041602 25.461273063653184 24.451222561128056 120-124 23.395 27.27 23.885 25.45 125-129 23.075000000000003 27.73 24.19 25.005 130-134 23.169999999999998 27.339999999999996 24.365000000000002 25.124999999999996 135-139 22.34 27.845 25.55 24.265 140-144 23.150000000000002 27.55 24.2 25.1 145-149 22.63 27.825 23.785 25.759999999999998 150-151 23.0875 26.637499999999996 24.05 26.224999999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.5 5 1.0 6 1.0 7 2.5 8 2.5 9 2.0 10 1.5 11 1.0 12 1.0 13 0.5 14 2.0 15 3.0 16 2.0 17 2.0 18 1.0 19 0.0 20 1.0 21 1.5 22 0.5 23 0.0 24 0.0 25 0.0 26 0.0 27 3.5 28 7.0 29 17.5 30 28.5 31 43.0 32 52.0 33 49.5 34 64.5 35 84.5 36 102.0 37 143.5 38 153.5 39 133.0 40 139.0 41 142.5 42 128.5 43 115.5 44 136.0 45 158.5 46 166.0 47 179.5 48 180.5 49 177.0 50 173.0 51 150.5 52 152.5 53 161.5 54 135.5 55 116.0 56 102.5 57 88.0 58 82.0 59 72.5 60 52.0 61 34.0 62 32.0 63 32.0 64 29.0 65 24.0 66 18.0 67 14.0 68 12.5 69 13.0 70 15.5 71 13.5 72 8.5 73 7.5 74 5.0 75 3.5 76 6.0 77 5.0 78 3.0 79 3.5 80 1.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.475 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.005 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.3 #Duplication Level Percentage of deduplicated Percentage of total 1 89.68710089399745 70.22500000000001 2 5.747126436781609 9.0 3 1.7879948914431671 4.2 4 1.053639846743295 3.3000000000000003 5 0.6704980842911877 2.625 6 0.19157088122605362 0.8999999999999999 7 0.28735632183908044 1.575 8 0.09578544061302681 0.6 9 0.1277139208173691 0.8999999999999999 >10 0.31928480204342274 3.675 >50 0.0 0.0 >100 0.031928480204342274 3.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCATGATCTCGTATGC 120 3.0 TruSeq Adapter, Index 6 (97% over 37bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 27 0.675 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 19 0.475 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 19 0.475 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 13 0.325 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 12 0.3 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 12 0.3 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 12 0.3 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 12 0.3 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 11 0.27499999999999997 No Hit GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT 10 0.25 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 9 0.22499999999999998 No Hit GCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTG 9 0.22499999999999998 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 9 0.22499999999999998 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 9 0.22499999999999998 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 8 0.2 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 8 0.2 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 8 0.2 No Hit CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT 7 0.17500000000000002 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 7 0.17500000000000002 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 7 0.17500000000000002 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 7 0.17500000000000002 No Hit GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT 7 0.17500000000000002 No Hit CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT 7 0.17500000000000002 No Hit GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT 7 0.17500000000000002 No Hit GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG 7 0.17500000000000002 No Hit GAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCC 7 0.17500000000000002 No Hit GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT 6 0.15 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 6 0.15 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 6 0.15 No Hit GGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTAT 6 0.15 No Hit GCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTT 6 0.15 No Hit GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA 6 0.15 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 5 0.125 No Hit GGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATA 5 0.125 No Hit CAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTAT 5 0.125 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 5 0.125 No Hit CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT 5 0.125 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 5 0.125 No Hit GTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATA 5 0.125 No Hit TTCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACA 5 0.125 No Hit CCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAG 5 0.125 No Hit CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG 5 0.125 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 5 0.125 No Hit GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG 5 0.125 No Hit CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA 5 0.125 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 5 0.125 No Hit GCGGTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTAT 5 0.125 No Hit TGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATC 5 0.125 No Hit GGCCCACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGT 5 0.125 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 5 0.125 No Hit GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA 5 0.125 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 5 0.125 No Hit GCCCACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.07500000000000001 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1625 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.5375 0.0 0.0 0.0 0.0 86-87 0.6875 0.0 0.0 0.0 0.0 88-89 0.8625 0.0 0.0 0.0 0.0 90-91 1.225 0.0 0.0 0.0 0.0 92-93 1.425 0.0 0.0 0.0 0.0 94-95 1.6375000000000002 0.0 0.0 0.0 0.0 96-97 2.0875 0.0 0.0 0.0 0.0 98-99 2.525 0.0 0.0 0.0 0.0 100-101 2.8625 0.0 0.0 0.0 0.0 102-103 3.3 0.0 0.0 0.0 0.0 104-105 3.8 0.0 0.0 0.0 0.0 106-107 4.425000000000001 0.0 0.0 0.0 0.0 108-109 4.9875 0.0 0.0 0.0 0.0 110-111 5.6375 0.0 0.0 0.0 0.0 112-113 6.1375 0.0 0.0 0.0 0.0 114-115 6.800000000000001 0.0 0.0 0.0 0.0 116-117 7.575 0.0 0.0 0.0 0.0 118-119 8.25 0.0 0.0 0.0 0.0 120-121 8.95 0.0 0.0 0.0 0.0 122-123 9.6375 0.0 0.0 0.0 0.0 124-125 10.475 0.0 0.0 0.0 0.0 126-127 11.3625 0.0 0.0 0.0 0.0 128-129 12.0875 0.0 0.0 0.0 0.0 130-131 13.3 0.0 0.0 0.0 0.0 132-133 14.0375 0.0 0.0 0.0 0.0 134-135 15.1125 0.0 0.0 0.0 0.0 136-137 16.0 0.0 0.0 0.0 0.0 138-139 17.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCTTGA 20 0.0059376103 28.9975 55-59 CTGCTTG 20 0.0059376103 28.9975 55-59 AAAAAAA 210 6.742266E-7 9.665833 65-69 >>END_MODULE SRR5578505 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578505_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8585 33.0 32.0 34.0 28.0 34.0 2 31.941 33.0 33.0 34.0 30.0 34.0 3 31.77925 33.0 33.0 34.0 29.0 34.0 4 31.76775 33.0 33.0 34.0 28.0 34.0 5 31.78425 33.0 33.0 34.0 28.0 34.0 6 35.79 38.0 37.0 38.0 31.0 38.0 7 35.6525 38.0 37.0 38.0 29.0 38.0 8 35.7315 38.0 37.0 38.0 31.0 38.0 9 35.62875 38.0 37.0 38.0 29.0 38.0 10-14 35.54495 38.0 37.0 38.0 29.4 38.0 15-19 35.3959 38.0 37.0 38.0 29.0 38.0 20-24 35.1948 38.0 37.0 38.0 28.4 38.0 25-29 35.03705 38.0 36.8 38.0 27.8 38.0 30-34 34.65785 38.0 36.0 38.0 26.2 38.0 35-39 34.4078 38.0 36.0 38.0 25.0 38.0 40-44 34.32605 38.0 35.4 38.0 25.4 38.0 45-49 33.96165 38.0 35.0 38.0 17.6 38.0 50-54 33.65240000000001 38.0 34.0 38.0 16.0 38.0 55-59 33.441250000000004 38.0 34.0 38.0 16.0 38.0 60-64 33.2598 38.0 34.0 38.0 16.0 38.0 65-69 32.638 38.0 32.6 38.0 15.8 38.0 70-74 31.883699999999997 37.6 31.0 38.0 15.0 38.0 75-79 31.331750000000007 37.0 29.2 38.0 15.0 38.0 80-84 30.955399999999997 37.0 29.0 38.0 13.8 38.0 85-89 30.245299999999997 36.6 26.8 38.0 13.0 38.0 90-94 29.625349999999997 36.0 25.0 38.0 8.6 38.0 95-99 28.932399999999994 35.4 23.4 38.0 2.0 38.0 100-104 28.123400000000004 35.0 17.4 38.0 2.0 38.0 105-109 27.281799999999997 34.0 15.0 38.0 2.0 38.0 110-114 26.15255 33.6 14.8 38.0 2.0 38.0 115-119 25.2627 32.8 13.8 38.0 2.0 38.0 120-124 24.10525 31.0 13.0 37.2 2.0 38.0 125-129 22.91205 28.4 4.2 36.6 2.0 38.0 130-134 21.24375 24.6 2.0 35.6 2.0 38.0 135-139 19.6132 21.0 2.0 35.0 2.0 38.0 140-144 17.705750000000002 14.4 2.0 34.4 2.0 38.0 145-149 15.3762 4.2 2.0 33.0 2.0 38.0 150-151 11.437125 2.0 2.0 26.0 2.0 35.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41.0 3 18.0 4 17.0 5 6.0 6 15.0 7 11.0 8 11.0 9 16.0 10 15.0 11 18.0 12 25.0 13 44.0 14 34.0 15 35.0 16 45.0 17 52.0 18 49.0 19 52.0 20 54.0 21 58.0 22 78.0 23 75.0 24 108.0 25 114.0 26 105.0 27 153.0 28 146.0 29 198.0 30 188.0 31 245.0 32 262.0 33 317.0 34 368.0 35 410.0 36 434.0 37 183.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.449999999999996 19.175 14.05 25.324999999999996 2 27.995996997748314 26.670002501876404 24.418313735301474 20.915686765073804 3 23.60450563204005 23.554443053817273 30.538172715894866 22.302878598247812 4 25.33166458072591 30.137672090112638 20.250312891113893 24.28035043804756 5 30.21276595744681 31.314142678347935 20.175219023779725 18.29787234042553 6 26.21310655327664 32.16608304152076 21.48574287143572 20.135067533766886 7 21.966474856142106 23.567675756817614 32.849637227920944 21.61621215911934 8 22.742056542406804 27.09532149111834 24.59344508381286 25.569176882662 9 25.256570713391742 24.080100125156445 26.608260325406757 24.055068836045056 10-14 26.08499774740952 26.43039495419733 22.656054462632028 24.828552835761126 15-19 26.27441161742614 25.30796194291437 25.292939409113668 23.124687030545818 20-24 26.469998998297108 26.995893018130822 23.79044375438245 22.743664229189623 25-29 25.80015026296018 27.222639619333833 23.911845730027547 23.065364387678436 30-34 27.51953516329393 25.806451612903224 24.76457623722701 21.909436986575837 35-39 25.812590774778382 24.926128111383782 25.70741723844343 23.553863875394402 40-44 27.43203324488059 25.12391728833926 24.75341711310269 22.690632353677465 45-49 25.64462023732038 24.693336003604866 25.163971361337804 24.498072397736944 50-54 24.795954133493566 24.88608482299334 27.519903860597868 22.798057182915226 55-59 23.31614001702639 26.72141819820722 27.29731083178927 22.665130952977115 60-64 23.487580128205128 27.468950320512818 26.52744391025641 22.516025641025642 65-69 22.54946155772602 28.615076383671422 26.225895316804408 22.609566741798144 70-74 23.142027243589745 28.275240384615387 25.806290064102566 22.776442307692307 75-79 23.07653776798237 28.456221198156683 25.01502704868764 23.452213985173312 80-84 23.372069725505913 28.040472851132037 25.125225405730315 23.462232017631738 85-89 23.48226808254859 27.73993187737928 26.172109797635745 22.605690242436385 90-94 24.236354531797698 28.28242363545318 25.59839759639459 21.88282423635453 95-99 23.567708333333336 28.525641025641026 25.49078525641026 22.415865384615387 100-104 24.256384576865297 28.718077115673513 24.817225838758137 22.208312468703053 105-109 24.10839511120016 30.139250651172112 23.787818072530555 21.964536165097176 110-114 23.115452041071876 29.070874029551714 24.598046581517654 23.215627347858753 115-119 24.022038567493112 29.7771099423992 23.651389932381665 22.54946155772602 120-124 24.660554135978757 30.036574978706348 23.332832306227765 21.970038579087127 125-129 24.606831613743363 29.725533406791545 23.42482219773615 22.242812781728936 130-134 25.865264212371653 28.259454044578014 24.232406711745554 21.642875031304783 135-139 25.773970544033663 28.99509067227733 23.46458270714357 21.76635607654544 140-144 26.32343366554816 29.23323483748185 22.857715230129713 21.585616266840287 145-149 26.883604505632043 28.7459324155194 22.73842302878598 21.632040050062578 150-151 27.92240300375469 29.799749687108886 20.95118898623279 21.32665832290363 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 1.5 2 1.5 3 1.0 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.5 16 1.0 17 0.5 18 0.0 19 0.0 20 0.5 21 1.0 22 1.5 23 2.0 24 1.0 25 1.0 26 1.0 27 2.0 28 3.0 29 5.5 30 15.5 31 24.5 32 28.0 33 27.5 34 44.0 35 64.5 36 72.5 37 112.0 38 143.0 39 141.0 40 181.5 41 177.5 42 129.0 43 130.5 44 133.5 45 134.5 46 139.0 47 143.5 48 165.0 49 185.5 50 176.5 51 156.5 52 155.5 53 166.5 54 174.0 55 154.0 56 124.5 57 108.0 58 90.5 59 75.0 60 55.0 61 42.5 62 38.0 63 38.0 64 31.5 65 24.0 66 23.5 67 20.5 68 20.0 69 18.0 70 17.5 71 15.5 72 10.5 73 10.0 74 9.0 75 5.5 76 4.5 77 3.5 78 4.5 79 4.0 80 1.0 81 2.0 82 2.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.125 4 0.125 5 0.125 6 0.05 7 0.075 8 0.075 9 0.125 10-14 0.11499999999999999 15-19 0.15 20-24 0.16999999999999998 25-29 0.17500000000000002 30-34 0.18 35-39 0.165 40-44 0.135 45-49 0.135 50-54 0.145 55-59 0.155 60-64 0.16 65-69 0.17500000000000002 70-74 0.16 75-79 0.18 80-84 0.18 85-89 0.18 90-94 0.15 95-99 0.16 100-104 0.15 105-109 0.18 110-114 0.17500000000000002 115-119 0.17500000000000002 120-124 0.20500000000000002 125-129 0.16999999999999998 130-134 0.17500000000000002 135-139 0.19 140-144 0.165 145-149 0.125 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.5 #Duplication Level Percentage of deduplicated Percentage of total 1 89.16129032258064 69.1 2 5.967741935483871 9.25 3 2.0 4.65 4 1.2258064516129032 3.8 5 0.5161290322580645 2.0 6 0.1935483870967742 0.8999999999999999 7 0.1935483870967742 1.05 8 0.03225806451612903 0.2 9 0.03225806451612903 0.22499999999999998 >10 0.6451612903225806 7.199999999999999 >50 0.03225806451612903 1.625 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 65 1.625 Illumina Single End PCR Primer 1 (100% over 50bp) TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 26 0.65 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 21 0.525 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 19 0.475 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 17 0.42500000000000004 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 16 0.4 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 16 0.4 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 15 0.375 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 15 0.375 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 15 0.375 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 14 0.35000000000000003 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 14 0.35000000000000003 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 14 0.35000000000000003 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 12 0.3 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 11 0.27499999999999997 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 11 0.27499999999999997 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 11 0.27499999999999997 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 11 0.27499999999999997 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 10 0.25 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 10 0.25 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 10 0.25 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 9 0.22499999999999998 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 8 0.2 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 7 0.17500000000000002 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 7 0.17500000000000002 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 7 0.17500000000000002 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 7 0.17500000000000002 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 7 0.17500000000000002 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 7 0.17500000000000002 No Hit GCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAG 6 0.15 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 6 0.15 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 6 0.15 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 6 0.15 No Hit CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA 6 0.15 No Hit GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG 6 0.15 No Hit GTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC 5 0.125 No Hit GACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCA 5 0.125 No Hit GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG 5 0.125 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 5 0.125 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 5 0.125 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 5 0.125 No Hit TAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTT 5 0.125 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 5 0.125 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 5 0.125 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 5 0.125 No Hit CAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTC 5 0.125 No Hit CACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGG 5 0.125 No Hit TGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCC 5 0.125 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 5 0.125 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 5 0.125 No Hit AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0125 0.0 0.0 0.0 20-21 0.0 0.025 0.0 0.0 0.0 22-23 0.0 0.025 0.0 0.0 0.0 24-25 0.0 0.025 0.0 0.0 0.0 26-27 0.0 0.025 0.0 0.0 0.0 28-29 0.0 0.025 0.0 0.0 0.0 30-31 0.0 0.025 0.0 0.0 0.0 32-33 0.0 0.025 0.0 0.0 0.0 34-35 0.0 0.025 0.0 0.0 0.0 36-37 0.0 0.025 0.0 0.0 0.0 38-39 0.0 0.025 0.0 0.0 0.0 40-41 0.0 0.025 0.0 0.0 0.0 42-43 0.0 0.025 0.0 0.0 0.0 44-45 0.0 0.025 0.0 0.0 0.0 46-47 0.0 0.025 0.0 0.0 0.0 48-49 0.0 0.025 0.0 0.0 0.0 50-51 0.0 0.025 0.0 0.0 0.0 52-53 0.0 0.025 0.0 0.0 0.0 54-55 0.0 0.025 0.0 0.0 0.0 56-57 0.025 0.025 0.0 0.0 0.0 58-59 0.025 0.025 0.0 0.0 0.0 60-61 0.025 0.025 0.0 0.0 0.0 62-63 0.025 0.025 0.0 0.0 0.0 64-65 0.025 0.025 0.0 0.0 0.0 66-67 0.025 0.025 0.0 0.0 0.0 68-69 0.05 0.025 0.0 0.0 0.0 70-71 0.05 0.025 0.0 0.0 0.0 72-73 0.07500000000000001 0.025 0.0 0.0 0.0 74-75 0.125 0.025 0.0 0.0 0.0 76-77 0.175 0.025 0.0 0.0 0.0 78-79 0.225 0.025 0.0 0.0 0.0 80-81 0.2875 0.025 0.0 0.0 0.0 82-83 0.3375 0.025 0.0 0.0 0.0 84-85 0.48750000000000004 0.025 0.0 0.0 0.0 86-87 0.6 0.025 0.0 0.0 0.0 88-89 0.7 0.025 0.0 0.0 0.0 90-91 0.9875 0.025 0.0 0.0 0.0 92-93 1.1375000000000002 0.025 0.0 0.0 0.0 94-95 1.2999999999999998 0.025 0.0 0.0 0.0 96-97 1.5375 0.025 0.0 0.0 0.0 98-99 1.85 0.025 0.0 0.0 0.0 100-101 2.0625 0.025 0.0 0.0 0.0 102-103 2.275 0.025 0.0 0.0 0.0 104-105 2.625 0.025 0.0 0.0 0.0 106-107 3.1125 0.025 0.0 0.0 0.0 108-109 3.4625000000000004 0.025 0.0 0.0 0.0 110-111 3.9125 0.025 0.0 0.0 0.0 112-113 4.25 0.025 0.0 0.0 0.0 114-115 4.6 0.025 0.0 0.0 0.0 116-117 5.0375 0.025 0.0 0.0 0.0 118-119 5.4375 0.025 0.0 0.0 0.0 120-121 5.8625 0.025 0.0 0.0 0.0 122-123 6.1875 0.025 0.0 0.0 0.0 124-125 6.675000000000001 0.025 0.0 0.0 0.0 126-127 7.2875 0.025 0.0 0.0 0.0 128-129 7.675000000000001 0.025 0.0 0.0 0.0 130-131 8.3 0.025 0.0 0.0 0.0 132-133 8.7375 0.025 0.0 0.0 0.0 134-135 9.3875 0.025 0.0 0.0 0.0 136-137 9.8625 0.025 0.0 0.0 0.0 138-139 10.425 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAAAA 395 4.5508827E-4 5.5063295 60-64 >>END_MODULE Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra Read 1093175 spots for SRR5578505.sra Written 1093175 spots for SRR5578505.sra SRR ids: ['SRR5578505.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_oe110_g_ SRR5578505.sra spots: 21863500 blocks: [[1, 1093175], [1093176, 2186350], [2186351, 3279525], [3279526, 4372700], [4372701, 5465875], [5465876, 6559050], [6559051, 7652225], [7652226, 8745400], [8745401, 9838575], [9838576, 10931750], [10931751, 12024925], [12024926, 13118100], [13118101, 14211275], [14211276, 15304450], [15304451, 16397625], [16397626, 17490800], [17490801, 18583975], [18583976, 19677150], [19677151, 20770325], [20770326, 21863500]] SRR5578505 file size 7387122 SRR5578505 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578505 SRR5578505_1.fastq SRR5578505_2.fastq Input file: SRR5578505_1.fastq Paired file: SRR5578505_2.fastq trimmed: SRR5578505-trimmed-pair1.fastq, SRR5578505-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 21:18:11 2024 >> started Mon Dec 9 21:19:32 2024 >> done (80.987s) 21863500 read pairs processed; of these: 78534 ( 0.36%) short read pairs filtered out after trimming by size control 756634 ( 3.46%) empty read pairs filtered out after trimming by size control 21028332 (96.18%) read pairs available; of these: 12290286 (58.45%) trimmed read pairs available after processing 8738046 (41.55%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 26 0.00% 19 27 0.00% 20 53 0.00% 21 42 0.00% 22 57 0.00% 23 51 0.00% 24 87 0.00% 25 48 0.00% 26 65 0.00% 27 67 0.00% 28 55 0.00% 29 51 0.00% 30 55 0.00% 31 85 0.00% 32 78 0.00% 33 60 0.00% 34 81 0.00% 35 96 0.00% 36 95 0.00% 37 95 0.00% 38 103 0.00% 39 99 0.00% 40 143 0.00% 41 142 0.00% 42 182 0.00% 43 227 0.00% 44 321 0.00% 45 377 0.00% 46 483 0.00% 47 470 0.00% 48 591 0.00% 49 618 0.00% 50 847 0.00% 51 956 0.00% 52 945 0.00% 53 965 0.00% 54 977 0.00% 55 1166 0.01% 56 1300 0.01% 57 1473 0.01% 58 1770 0.01% 59 1584 0.01% 60 1616 0.01% 61 1867 0.01% 62 2161 0.01% 63 2680 0.01% 64 3294 0.02% 65 4542 0.02% 66 8400 0.04% 67 20639 0.10% 68 32790 0.16% 69 45163 0.21% 70 50293 0.24% 71 17823 0.08% 72 9538 0.05% 73 8073 0.04% 74 8412 0.04% 75 9209 0.04% 76 9564 0.05% 77 10724 0.05% 78 11589 0.06% 79 12914 0.06% 80 13875 0.07% 81 15095 0.07% 82 17978 0.09% 83 20073 0.10% 84 24781 0.12% 85 28555 0.14% 86 30072 0.14% 87 32282 0.15% 88 35250 0.17% 89 37126 0.18% 90 38580 0.18% 91 39524 0.19% 92 42100 0.20% 93 44563 0.21% 94 46693 0.22% 95 48998 0.23% 96 50469 0.24% 97 53276 0.25% 98 55012 0.26% 99 58625 0.28% 100 63013 0.30% 101 64556 0.31% 102 66279 0.32% 103 70724 0.34% 104 74580 0.35% 105 77899 0.37% 106 81030 0.39% 107 82599 0.39% 108 85799 0.41% 109 85322 0.41% 110 88251 0.42% 111 90833 0.43% 112 94398 0.45% 113 103274 0.49% 114 107505 0.51% 115 110319 0.52% 116 110559 0.53% 117 111149 0.53% 118 111168 0.53% 119 112153 0.53% 120 116936 0.56% 121 119035 0.57% 122 123254 0.59% 123 129799 0.62% 124 133202 0.63% 125 134165 0.64% 126 139403 0.66% 127 138569 0.66% 128 135475 0.64% 129 142661 0.68% 130 143793 0.68% 131 145061 0.69% 132 149069 0.71% 133 153196 0.73% 134 155871 0.74% 135 156738 0.75% 136 160293 0.76% 137 162509 0.77% 138 171468 0.82% 139 177232 0.84% 140 183515 0.87% 141 188157 0.89% 142 208979 0.99% 143 220484 1.05% 144 236902 1.13% 145 265899 1.26% 146 303393 1.44% 147 369235 1.76% 148 493628 2.35% 149 833312 3.96% 150 3286412 15.63% 151 8738046 41.55% 21028332 reads passed initial QC criterion=sequence-density sequence-density=0.59 sequence-density-rank=1 fanout-score=22.23 fanout-score-rank=3 prefix-density=6.72 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=70.12 fanout-score-rank=1 prefix-density=0.54 prefix-fanout=1.3 sequence=TTTTTTTTCTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATTCCCGAGGCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCCCAGAGACGGGCGGAGGCTCTTAACCGTACGAGACAATCTCCA criterion=sequence-density sequence-density=0.93 sequence-density-rank=1 fanout-score=4.96 fanout-score-rank=12 prefix-density=4.52 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.13 sequence-density-rank=17 fanout-score=76.95 fanout-score-rank=1 prefix-density=9.88 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR5578505 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 21:22:21 Started mapping on | Dec 09 21:22:21 Finished on | Dec 09 22:09:58 Mapping speed, Million of reads per hour | 26.50 Number of input reads | 21028332 Average input read length | 281 UNIQUE READS: Uniquely mapped reads number | 8218935 Uniquely mapped reads % | 39.09% Average mapped length | 278.72 Number of splices: Total | 5048102 Number of splices: Annotated (sjdb) | 4686580 Number of splices: GT/AG | 4978047 Number of splices: GC/AG | 60709 Number of splices: AT/AC | 2315 Number of splices: Non-canonical | 7031 Mismatch rate per base, % | 0.15% Deletion rate per base | 0.01% Deletion average length | 1.51 Insertion rate per base | 0.00% Insertion average length | 1.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 383005 % of reads mapped to multiple loci | 1.82% Number of reads mapped to too many loci | 86674 % of reads mapped to too many loci | 0.41% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 56.63% % of reads unmapped: other | 2.05% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 12445137 12445137 12445137 N_multimapping 383005 383005 383005 N_noFeature 392102 7914932 475716 N_ambiguous 245034 934 26034 UnstrandedReadsAssigned:7581799 PositiveStrandReadsAssigned:303069 NegativeStrandReadsAssigned:7717185 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=136 echo kmer=131 SRR5578505 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578505-trimmed-pair1.fastq SRR5578505-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,028,332 reads, 7,849,102 reads pseudoaligned [quant] estimated average fragment length: 190.343 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,072 rounds 52973 SRR5578505.ke.tsv 35125 SRR5578505.se.tsv 88098 total ==> SRR5578505.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 746.828 0 0 PNS24247 1044 854.657 0 0 PNS24249 1928 1738.66 52.4209 5.00299 PNS24246 1044 854.657 0 0 PNS24248 1044 854.657 0 0 PNS24244 1471 1281.66 174.579 22.6027 PNS24243 293 124.441 0 0 KQK14069 1603 1413.66 2045.89 240.148 KQK14071 474 289.135 12.2288 7.01814 ==> SRR5578505.se.tsv <== BRADI_1g14170v3 2101 BRADI_1g53295v3 16 BRADI_1g59795v3 101 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 331 BRADI_1g74790v3 299 BRADI_1g09890v3 0 BRADI_1g77505v3 116 BRADI_1g48960v3 0 SRR5578505 completed mapping pipeline successfully