Starting /dee2/code/volunteer_pipeline.sh SRR5578516 current disk space = 1522500120576 free memory = 1593310400 SRR5578516 SRAfilesize dbda92cd66ef8fee3c35593ad5f98fbe SRR5578516.sra SRR5578516.sra file validated SRR5578516 is paired end SRR5578516 is conventional basespace SRR5578516 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578516_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.54675 34.0 34.0 34.0 33.0 34.0 2 33.497 34.0 34.0 34.0 33.0 34.0 3 33.594 34.0 34.0 34.0 33.0 34.0 4 33.6035 34.0 34.0 34.0 33.0 34.0 5 33.58675 34.0 34.0 34.0 33.0 34.0 6 37.32325 38.0 38.0 38.0 36.0 38.0 7 37.55625 38.0 38.0 38.0 37.0 38.0 8 37.62875 38.0 38.0 38.0 38.0 38.0 9 37.568 38.0 38.0 38.0 38.0 38.0 10-14 37.623900000000006 38.0 38.0 38.0 38.0 38.0 15-19 37.589800000000004 38.0 38.0 38.0 38.0 38.0 20-24 37.561 38.0 38.0 38.0 38.0 38.0 25-29 37.48615 38.0 38.0 38.0 38.0 38.0 30-34 37.44005 38.0 38.0 38.0 38.0 38.0 35-39 37.3766 38.0 38.0 38.0 37.8 38.0 40-44 37.3 38.0 38.0 38.0 37.0 38.0 45-49 37.318 38.0 38.0 38.0 37.0 38.0 50-54 37.3055 38.0 38.0 38.0 37.0 38.0 55-59 37.2146 38.0 38.0 38.0 37.0 38.0 60-64 37.246300000000005 38.0 38.0 38.0 37.0 38.0 65-69 37.17999999999999 38.0 38.0 38.0 37.0 38.0 70-74 37.10915 38.0 38.0 38.0 37.0 38.0 75-79 36.9745 38.0 38.0 38.0 36.6 38.0 80-84 36.8717 38.0 38.0 38.0 36.0 38.0 85-89 36.825599999999994 38.0 38.0 38.0 36.0 38.0 90-94 36.75685 38.0 38.0 38.0 36.0 38.0 95-99 36.66785 38.0 38.0 38.0 35.4 38.0 100-104 36.60705 38.0 38.0 38.0 35.4 38.0 105-109 36.517849999999996 38.0 38.0 38.0 35.0 38.0 110-114 36.53795 38.0 38.0 38.0 35.0 38.0 115-119 36.3488 38.0 38.0 38.0 34.4 38.0 120-124 36.21210000000001 38.0 38.0 38.0 34.0 38.0 125-129 36.0804 38.0 38.0 38.0 34.0 38.0 130-134 35.966499999999996 38.0 38.0 38.0 33.4 38.0 135-139 35.69205 38.0 37.6 38.0 32.6 38.0 140-144 35.529 38.0 36.8 38.0 32.6 38.0 145-149 35.05835 38.0 36.0 38.0 31.0 38.0 150-151 32.06525 36.5 32.0 38.0 15.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 4.0 8 0.0 9 4.0 10 2.0 11 3.0 12 1.0 13 1.0 14 2.0 15 5.0 16 3.0 17 5.0 18 10.0 19 8.0 20 4.0 21 2.0 22 7.0 23 6.0 24 5.0 25 9.0 26 11.0 27 13.0 28 17.0 29 26.0 30 18.0 31 26.0 32 45.0 33 67.0 34 85.0 35 130.0 36 366.0 37 3114.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.9 12.8 11.125 33.175 2 27.930861723446892 13.927855711422845 30.135270541082164 28.006012024048093 3 24.2 17.575 26.275 31.95 4 24.425 26.875 23.95 24.75 5 25.55 29.15 26.674999999999997 18.625 6 21.525 32.35 25.15 20.974999999999998 7 14.575 25.624999999999996 42.025 17.775 8 20.549999999999997 25.724999999999998 30.099999999999998 23.625 9 18.45 24.825 34.4 22.325 10-14 22.89 28.51 26.064999999999998 22.535 15-19 22.295 27.175 26.919999999999998 23.61 20-24 22.400000000000002 26.755000000000003 27.33 23.515 25-29 21.615000000000002 27.115000000000002 27.76 23.51 30-34 21.29 27.985 27.139999999999997 23.585 35-39 22.02 26.919999999999998 27.33 23.73 40-44 23.575 26.565 26.395000000000003 23.465 45-49 22.767276727672765 27.557755775577558 27.21272127212721 22.46224622462246 50-54 23.555 27.195000000000004 25.779999999999998 23.47 55-59 22.28722872287229 27.032703270327037 27.012701270127014 23.667366736673667 60-64 21.785 28.325 26.16 23.73 65-69 22.071621486445935 27.613283985195558 26.052815844753425 24.262278683605082 70-74 22.827282728272827 27.49274927492749 25.837583758375835 23.84238423842384 75-79 22.384999999999998 26.445 26.445 24.725 80-84 22.189999999999998 27.43 25.515 24.865000000000002 85-89 23.769753950790157 26.42028405681136 26.415283056611322 23.39467893578716 90-94 22.99 26.35 26.540000000000003 24.12 95-99 22.14 25.900000000000002 27.72 24.240000000000002 100-104 23.525 26.179999999999996 26.179999999999996 24.115000000000002 105-109 22.78 27.18 26.33 23.71 110-114 22.03 26.51 25.935000000000002 25.525 115-119 21.94 27.71 25.679999999999996 24.67 120-124 23.294999999999998 27.82 24.25 24.635 125-129 23.11 27.26 24.305 25.324999999999996 130-134 23.345 27.095000000000002 24.89 24.67 135-139 22.595000000000002 27.345000000000002 25.83 24.23 140-144 23.03 27.48 24.740000000000002 24.75 145-149 22.905 27.474999999999998 24.395 25.224999999999998 150-151 23.1125 26.0375 24.962500000000002 25.887500000000003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 1.0 5 2.0 6 2.5 7 1.0 8 0.5 9 1.0 10 0.5 11 1.0 12 1.0 13 1.0 14 2.0 15 1.5 16 0.5 17 0.0 18 0.5 19 1.0 20 0.5 21 1.5 22 2.0 23 1.0 24 1.0 25 2.5 26 2.5 27 2.5 28 6.5 29 10.5 30 23.0 31 44.0 32 49.0 33 54.5 34 75.0 35 93.5 36 116.5 37 147.0 38 154.0 39 125.5 40 132.5 41 156.0 42 130.5 43 120.0 44 135.0 45 144.5 46 156.0 47 165.5 48 167.5 49 180.5 50 177.0 51 149.5 52 142.5 53 154.5 54 143.5 55 119.5 56 112.5 57 97.0 58 80.5 59 68.5 60 57.0 61 49.0 62 37.0 63 27.5 64 28.0 65 24.0 66 16.0 67 15.5 68 15.0 69 10.5 70 8.0 71 9.0 72 9.0 73 8.5 74 9.0 75 7.0 76 3.0 77 0.0 78 0.5 79 1.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.2 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.01 50-54 0.0 55-59 0.01 60-64 0.0 65-69 0.03 70-74 0.01 75-79 0.0 80-84 0.0 85-89 0.02 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.35 #Duplication Level Percentage of deduplicated Percentage of total 1 90.7103825136612 74.7 2 5.434122647237402 8.95 3 1.5482695810564664 3.8249999999999997 4 1.0321797207043109 3.4000000000000004 5 0.36429872495446264 1.5 6 0.21250758955676988 1.05 7 0.09107468123861566 0.525 8 0.18214936247723132 1.2 9 0.030358227079538558 0.22499999999999998 >10 0.3946569520340012 4.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 29 0.7250000000000001 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 22 0.5499999999999999 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTCATCTCGTATGC 16 0.4 TruSeq Adapter, Index 13 (97% over 37bp) GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 16 0.4 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 15 0.375 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 12 0.3 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 12 0.3 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 11 0.27499999999999997 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 11 0.27499999999999997 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 11 0.27499999999999997 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 10 0.25 No Hit GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA 10 0.25 No Hit GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA 10 0.25 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 9 0.22499999999999998 No Hit CAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTAT 8 0.2 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 8 0.2 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 8 0.2 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 8 0.2 No Hit GATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATG 8 0.2 No Hit GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG 8 0.2 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 7 0.17500000000000002 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 7 0.17500000000000002 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 7 0.17500000000000002 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 6 0.15 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 6 0.15 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 6 0.15 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 6 0.15 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 6 0.15 No Hit GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG 6 0.15 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 6 0.15 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 5 0.125 No Hit GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT 5 0.125 No Hit GGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAAT 5 0.125 No Hit GCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAAT 5 0.125 No Hit CTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGG 5 0.125 No Hit GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT 5 0.125 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 5 0.125 No Hit CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT 5 0.125 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 5 0.125 No Hit GTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGA 5 0.125 No Hit CATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAA 5 0.125 No Hit ACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.36250000000000004 0.0 0.0 0.0 0.0 84-85 0.48750000000000004 0.0 0.0 0.0 0.0 86-87 0.55 0.0 0.0 0.0 0.0125 88-89 0.575 0.0 0.0 0.0 0.025 90-91 0.625 0.0 0.0 0.0 0.025 92-93 0.825 0.0 0.0 0.0 0.025 94-95 1.0499999999999998 0.0 0.0 0.0 0.025 96-97 1.25 0.0 0.0 0.0 0.025 98-99 1.5125000000000002 0.0 0.0 0.0 0.025 100-101 1.8875 0.0 0.0 0.0 0.025 102-103 2.1125 0.0 0.0 0.0 0.025 104-105 2.375 0.0 0.0 0.0 0.025 106-107 2.7125 0.0 0.0 0.0 0.025 108-109 3.1 0.0 0.0 0.0 0.025 110-111 3.5625 0.0 0.0 0.0 0.025 112-113 3.9 0.0 0.0 0.0 0.025 114-115 4.4125 0.0 0.0 0.0 0.025 116-117 5.0 0.0 0.0 0.0 0.025 118-119 5.55 0.0 0.0 0.0 0.025 120-121 6.112500000000001 0.0 0.0 0.0 0.025 122-123 6.925000000000001 0.0 0.0 0.0 0.025 124-125 7.550000000000001 0.0 0.0 0.0 0.025 126-127 8.3875 0.0 0.0 0.0 0.025 128-129 9.2125 0.0 0.0 0.0 0.025 130-131 9.9375 0.0 0.0 0.0 0.025 132-133 10.7625 0.0 0.0 0.0 0.025 134-135 11.5 0.0 0.0 0.0 0.025 136-137 12.4875 0.0 0.0 0.0 0.025 138-139 13.4625 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR5578516 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5578516_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.043 33.0 33.0 34.0 31.0 34.0 2 32.1335 33.0 33.0 34.0 31.0 34.0 3 32.044 33.0 33.0 34.0 29.0 34.0 4 32.0435 33.0 33.0 34.0 31.0 34.0 5 32.12025 33.0 33.0 34.0 31.0 34.0 6 36.20875 38.0 38.0 38.0 33.0 38.0 7 36.01075 38.0 38.0 38.0 33.0 38.0 8 36.02475 38.0 38.0 38.0 33.0 38.0 9 36.0415 38.0 38.0 38.0 33.0 38.0 10-14 35.9619 38.0 38.0 38.0 33.0 38.0 15-19 35.84305 38.0 37.8 38.0 31.8 38.0 20-24 35.8295 38.0 37.6 38.0 32.2 38.0 25-29 35.73235 38.0 37.0 38.0 31.2 38.0 30-34 35.466300000000004 38.0 37.0 38.0 29.2 38.0 35-39 35.244099999999996 38.0 37.0 38.0 28.8 38.0 40-44 35.1705 38.0 36.6 38.0 28.8 38.0 45-49 34.90115 38.0 36.0 38.0 27.8 38.0 50-54 34.69085 38.0 36.0 38.0 27.0 38.0 55-59 34.411350000000006 38.0 35.6 38.0 25.4 38.0 60-64 34.18465 38.0 35.0 38.0 24.6 38.0 65-69 33.8899 38.0 34.4 38.0 19.2 38.0 70-74 33.535999999999994 38.0 34.0 38.0 16.0 38.0 75-79 33.17155 38.0 33.8 38.0 16.0 38.0 80-84 32.59865 37.8 32.6 38.0 15.0 38.0 85-89 32.156600000000005 37.0 30.6 38.0 15.0 38.0 90-94 31.565499999999997 37.0 29.2 38.0 15.0 38.0 95-99 30.768900000000002 36.2 27.6 38.0 14.0 38.0 100-104 30.15745 36.0 26.2 38.0 13.0 38.0 105-109 29.17645 35.0 23.2 38.0 10.8 38.0 110-114 28.3376 34.4 20.6 38.0 2.0 38.0 115-119 27.07595 34.0 15.0 38.0 2.0 38.0 120-124 26.0262 33.8 14.6 38.0 2.0 38.0 125-129 24.7518 32.0 13.4 37.6 2.0 38.0 130-134 22.9964 28.4 6.4 36.2 2.0 38.0 135-139 21.441200000000002 24.8 2.0 35.2 2.0 38.0 140-144 19.826100000000004 21.8 2.0 35.0 2.0 38.0 145-149 17.1459 12.6 2.0 33.6 2.0 38.0 150-151 12.857375000000001 2.0 2.0 29.5 2.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33.0 3 11.0 4 8.0 5 4.0 6 11.0 7 14.0 8 8.0 9 9.0 10 9.0 11 6.0 12 15.0 13 16.0 14 15.0 15 28.0 16 27.0 17 36.0 18 23.0 19 37.0 20 34.0 21 61.0 22 67.0 23 71.0 24 91.0 25 100.0 26 105.0 27 135.0 28 127.0 29 164.0 30 203.0 31 259.0 32 287.0 33 305.0 34 441.0 35 527.0 36 491.0 37 222.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.160040010002504 19.579894973743436 13.87846961740435 26.38159539884971 2 29.161451814768462 24.63078848560701 25.00625782227785 21.201501877346686 3 25.7135703555333 23.660490736104155 28.668002003004506 21.957936905358036 4 26.81522283425138 30.070105157736606 21.30696044066099 21.807711567351028 5 26.74011016524787 33.550325488232346 20.85628442663996 18.85327991987982 6 24.656164041010253 32.6081520380095 21.655413853463365 21.080270067516878 7 22.62828535669587 19.59949937421777 34.242803504380475 23.52941176470588 8 24.41161742613921 24.511767651477214 25.63845768652979 25.43815723585378 9 24.405506883604506 24.730913642052567 26.708385481852314 24.155193992490613 10-14 25.610855197276187 27.29821750450631 22.902062888043258 24.188864410174244 15-19 25.17398487958744 26.145296149802235 25.053822660591802 23.626896310018523 20-24 25.00625782227785 27.214017521902377 24.25531914893617 23.524405506883607 25-29 26.610279765777488 26.650317801911815 23.652469846354034 23.08693258595666 30-34 26.13681891025641 27.018229166666668 24.759615384615387 22.08533653846154 35-39 25.80774432700496 25.016280118218702 25.311826879727494 23.864148675048842 40-44 26.38325572079515 25.251614841520205 25.757348154824495 22.60778128286015 45-49 25.793849544225182 25.63357708103776 26.14444555744766 22.428127817289393 50-54 25.15021029441218 25.260364510314442 26.57220108151412 23.017224113759262 55-59 23.822159915886445 26.305512441796424 26.946377609773194 22.925950032543934 60-64 24.182646572873377 25.734741901567116 27.186702047764484 22.895909477795023 65-69 24.355315207050225 27.089279455210054 26.253066947073258 22.302338390666463 70-74 23.44164622240024 26.836228909027188 26.205377259300057 23.516747609272517 75-79 23.055736391406683 27.53768340928439 25.719865791977565 23.686714407331362 80-84 23.052663195835002 26.787144573488185 26.83219863836604 23.327993592310772 85-89 23.108486789431545 27.502001601281023 26.5162129703763 22.87329863891113 90-94 24.2865725443076 27.250425553219188 26.098928607189347 22.364073295283866 95-99 23.326490762529414 28.153006558854454 26.145296149802235 22.375206528813898 100-104 24.344606764058437 28.422053231939167 25.200120072043227 22.033219931959174 105-109 23.251389375657137 28.543533770590297 25.429329594953188 22.775747258799377 110-114 23.819774718397998 27.794743429286605 25.266583229036293 23.1188986232791 115-119 24.021423565922515 29.652617879667638 24.52197417158875 21.803984382821103 120-124 23.9729797348011 29.777332999749813 24.31823867900926 21.93144858643983 125-129 24.901126408010015 29.717146433041304 23.384230287859825 21.997496871088863 130-134 24.690863579474343 28.140175219023778 24.901126408010015 22.267834793491865 135-139 24.708589724348393 29.456200910500772 23.983190754915203 21.85201861023563 140-144 26.10066039623774 28.982389433660195 23.889333600160096 21.027616569941966 145-149 25.964087430600713 28.68503976391737 23.94838193367679 21.40249087180513 150-151 26.828353544193025 29.216152019002372 22.46530816352044 21.49018627328416 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 0.5 3 1.0 4 1.5 5 1.0 6 1.0 7 0.5 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 1.5 19 2.5 20 1.5 21 1.0 22 1.5 23 1.0 24 0.5 25 0.5 26 0.0 27 2.5 28 11.5 29 11.5 30 15.0 31 29.0 32 35.5 33 35.0 34 40.0 35 50.5 36 75.5 37 113.0 38 132.0 39 150.5 40 199.0 41 169.5 42 109.0 43 114.5 44 127.0 45 140.5 46 137.0 47 150.0 48 170.5 49 170.0 50 154.5 51 140.5 52 158.5 53 167.0 54 168.0 55 163.0 56 131.0 57 101.0 58 91.5 59 84.5 60 62.0 61 48.5 62 45.0 63 41.5 64 37.0 65 33.5 66 27.5 67 26.0 68 23.0 69 16.5 70 12.0 71 10.0 72 13.5 73 10.5 74 6.5 75 6.0 76 5.0 77 3.5 78 2.0 79 1.5 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.125 3 0.15 4 0.15 5 0.15 6 0.025 7 0.125 8 0.15 9 0.125 10-14 0.13999999999999999 15-19 0.135 20-24 0.125 25-29 0.095 30-34 0.16 35-39 0.185 40-44 0.145 45-49 0.16999999999999998 50-54 0.13999999999999999 55-59 0.135 60-64 0.135 65-69 0.145 70-74 0.135 75-79 0.155 80-84 0.12 85-89 0.08 90-94 0.13 95-99 0.135 100-104 0.06 105-109 0.135 110-114 0.125 115-119 0.11 120-124 0.075 125-129 0.125 130-134 0.125 135-139 0.055 140-144 0.06 145-149 0.034999999999999996 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 90.50868486352357 72.95 2 5.5521091811414385 8.95 3 1.7990074441687345 4.35 4 0.6823821339950372 2.1999999999999997 5 0.4032258064516129 1.625 6 0.21712158808933005 1.05 7 0.15508684863523575 0.8750000000000001 8 0.062034739454094295 0.4 9 0.18610421836228289 1.35 >10 0.4342431761786601 6.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 32 0.8 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 28 0.7000000000000001 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 23 0.575 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 21 0.525 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 19 0.475 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 16 0.4 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 16 0.4 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 16 0.4 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 16 0.4 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 16 0.4 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 14 0.35000000000000003 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 13 0.325 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 10 0.25 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 10 0.25 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 9 0.22499999999999998 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 9 0.22499999999999998 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 9 0.22499999999999998 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 9 0.22499999999999998 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 9 0.22499999999999998 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 9 0.22499999999999998 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 8 0.2 No Hit GCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAA 8 0.2 No Hit GAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGA 7 0.17500000000000002 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 7 0.17500000000000002 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 7 0.17500000000000002 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 7 0.17500000000000002 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 7 0.17500000000000002 No Hit CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA 6 0.15 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 6 0.15 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 6 0.15 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 6 0.15 No Hit CCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCG 6 0.15 No Hit AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG 6 0.15 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 6 0.15 No Hit AGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAG 5 0.125 No Hit CAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCG 5 0.125 No Hit GCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGT 5 0.125 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 5 0.125 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 5 0.125 No Hit GGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATA 5 0.125 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 5 0.125 No Hit TAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA 5 0.125 No Hit GGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCAC 5 0.125 No Hit GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG 5 0.125 No Hit CTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATC 5 0.125 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 5 0.125 No Hit CATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.2625 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.4 0.0 0.0 0.0 0.0 88-89 0.42500000000000004 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.6125 0.0 0.0 0.0 0.0 94-95 0.7875 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.125 0.0 0.0 0.0 0.0 100-101 1.3875 0.0 0.0 0.0 0.0 102-103 1.5625 0.0 0.0 0.0 0.0 104-105 1.7625 0.0 0.0 0.0 0.0 106-107 2.0 0.0 0.0 0.0 0.0 108-109 2.325 0.0 0.0 0.0 0.0 110-111 2.6500000000000004 0.0 0.0 0.0 0.0 112-113 2.85 0.0 0.0 0.0 0.0 114-115 3.1625 0.0 0.0 0.0 0.0 116-117 3.4875 0.0 0.0 0.0 0.0 118-119 3.8375 0.0 0.0 0.0 0.0 120-121 4.225 0.0 0.0 0.0 0.0 122-123 4.6625 0.0 0.0 0.0 0.0 124-125 5.0 0.0 0.0 0.0 0.0 126-127 5.5375 0.0 0.0 0.0 0.0 128-129 5.9625 0.0 0.0 0.0 0.0 130-131 6.4125 0.0 0.0 0.0 0.0 132-133 6.8875 0.0 0.0 0.0 0.0 134-135 7.2125 0.0 0.0 0.0 0.0 136-137 7.675 0.0 0.0 0.0 0.0 138-139 8.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCATAC 10 0.006830828 145.0 7 TCCCGGC 10 0.006830828 145.0 7 ACAATCC 10 0.006830828 145.0 3 >>END_MODULE Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932268 spots for SRR5578516.sra Written 932268 spots for SRR5578516.sra Read 932280 spots for SRR5578516.sra Written 932280 spots for SRR5578516.sra SRR ids: ['SRR5578516.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_48qx1nf5 SRR5578516.sra spots: 18645372 blocks: [[1, 932268], [932269, 1864536], [1864537, 2796804], [2796805, 3729072], [3729073, 4661340], [4661341, 5593608], [5593609, 6525876], [6525877, 7458144], [7458145, 8390412], [8390413, 9322680], [9322681, 10254948], [10254949, 11187216], [11187217, 12119484], [12119485, 13051752], [13051753, 13984020], [13984021, 14916288], [14916289, 15848556], [15848557, 16780824], [16780825, 17713092], [17713093, 18645372]] SRR5578516 file size 6296604 SRR5578516 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5578516 SRR5578516_1.fastq SRR5578516_2.fastq Input file: SRR5578516_1.fastq Paired file: SRR5578516_2.fastq trimmed: SRR5578516-trimmed-pair1.fastq, SRR5578516-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 21:55:53 2024 >> started Mon Dec 9 21:56:21 2024 >> done (27.493s) 18645372 read pairs processed; of these: 60948 ( 0.33%) short read pairs filtered out after trimming by size control 138500 ( 0.74%) empty read pairs filtered out after trimming by size control 18445924 (98.93%) read pairs available; of these: 10533052 (57.10%) trimmed read pairs available after processing 7912872 (42.90%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 7 0.00% 20 11 0.00% 21 8 0.00% 22 10 0.00% 23 14 0.00% 24 24 0.00% 25 8 0.00% 26 33 0.00% 27 21 0.00% 28 42 0.00% 29 32 0.00% 30 35 0.00% 31 45 0.00% 32 27 0.00% 33 34 0.00% 34 44 0.00% 35 46 0.00% 36 31 0.00% 37 43 0.00% 38 48 0.00% 39 64 0.00% 40 50 0.00% 41 62 0.00% 42 68 0.00% 43 74 0.00% 44 84 0.00% 45 111 0.00% 46 121 0.00% 47 154 0.00% 48 168 0.00% 49 204 0.00% 50 209 0.00% 51 291 0.00% 52 290 0.00% 53 346 0.00% 54 339 0.00% 55 383 0.00% 56 474 0.00% 57 464 0.00% 58 559 0.00% 59 627 0.00% 60 666 0.00% 61 732 0.00% 62 869 0.00% 63 946 0.01% 64 1178 0.01% 65 1322 0.01% 66 1797 0.01% 67 2546 0.01% 68 4156 0.02% 69 7923 0.04% 70 8869 0.05% 71 4337 0.02% 72 3552 0.02% 73 3576 0.02% 74 4006 0.02% 75 4288 0.02% 76 4561 0.02% 77 5059 0.03% 78 5592 0.03% 79 6271 0.03% 80 6970 0.04% 81 7671 0.04% 82 8997 0.05% 83 10143 0.05% 84 13456 0.07% 85 16062 0.09% 86 17274 0.09% 87 18830 0.10% 88 20245 0.11% 89 21240 0.12% 90 22581 0.12% 91 22946 0.12% 92 24106 0.13% 93 25436 0.14% 94 26885 0.15% 95 28773 0.16% 96 30163 0.16% 97 31847 0.17% 98 33178 0.18% 99 35087 0.19% 100 38206 0.21% 101 39628 0.21% 102 42043 0.23% 103 44358 0.24% 104 47645 0.26% 105 50157 0.27% 106 52349 0.28% 107 54050 0.29% 108 56022 0.30% 109 57192 0.31% 110 59285 0.32% 111 61998 0.34% 112 66164 0.36% 113 71928 0.39% 114 76057 0.41% 115 79656 0.43% 116 80281 0.44% 117 80873 0.44% 118 81397 0.44% 119 84018 0.46% 120 87848 0.48% 121 89018 0.48% 122 94635 0.51% 123 99582 0.54% 124 103088 0.56% 125 106202 0.58% 126 109423 0.59% 127 110250 0.60% 128 110127 0.60% 129 115152 0.62% 130 115170 0.62% 131 118449 0.64% 132 125108 0.68% 133 129001 0.70% 134 131678 0.71% 135 135380 0.73% 136 140460 0.76% 137 143940 0.78% 138 152002 0.82% 139 159535 0.86% 140 166230 0.90% 141 172209 0.93% 142 192983 1.05% 143 205148 1.11% 144 224370 1.22% 145 256067 1.39% 146 302165 1.64% 147 377698 2.05% 148 515805 2.80% 149 862598 4.68% 150 3158582 17.12% 151 7912872 42.90% 18445924 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=23.69 fanout-score-rank=2 prefix-density=5.70 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=46.65 fanout-score-rank=1 prefix-density=0.44 prefix-fanout=1.4 sequence=TTTTTTTTCTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATTCCCGAGGCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCCCAGAGACGGGCGGAGGCTCTTAACCGTACGAGACAATCTCC criterion=sequence-density sequence-density=0.76 sequence-density-rank=1 fanout-score=5.14 fanout-score-rank=10 prefix-density=3.85 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.12 sequence-density-rank=24 fanout-score=75.25 fanout-score-rank=1 prefix-density=8.74 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR5578516 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 21:58:06 Started mapping on | Dec 09 21:58:06 Finished on | Dec 09 22:29:03 Mapping speed, Million of reads per hour | 35.76 Number of input reads | 18445924 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 8150103 Uniquely mapped reads % | 44.18% Average mapped length | 283.98 Number of splices: Total | 5886010 Number of splices: Annotated (sjdb) | 5516798 Number of splices: GT/AG | 5804993 Number of splices: GC/AG | 71743 Number of splices: AT/AC | 3368 Number of splices: Non-canonical | 5906 Mismatch rate per base, % | 0.16% Deletion rate per base | 0.01% Deletion average length | 1.45 Insertion rate per base | 0.00% Insertion average length | 1.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 419796 % of reads mapped to multiple loci | 2.28% Number of reads mapped to too many loci | 76377 % of reads mapped to too many loci | 0.41% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 50.60% % of reads unmapped: other | 2.53% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9893782 9893782 9893782 N_multimapping 419796 419796 419796 N_noFeature 412896 7885617 477083 N_ambiguous 224672 912 25186 UnstrandedReadsAssigned:7512535 PositiveStrandReadsAssigned:263574 NegativeStrandReadsAssigned:7647834 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=144 echo kmer=139 SRR5578516 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR5578516-trimmed-pair1.fastq SRR5578516-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,445,924 reads, 7,811,219 reads pseudoaligned [quant] estimated average fragment length: 202.198 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,076 rounds 52973 SRR5578516.ke.tsv 35125 SRR5578516.se.tsv 88098 total ==> SRR5578516.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 735.057 3.90229 0.863519 PNS24247 1044 842.802 0 0 PNS24249 1928 1726.8 30.2285 2.84739 PNS24246 1044 842.802 0 0 PNS24248 1044 842.802 0 0 PNS24244 1471 1269.8 136.869 17.5325 PNS24243 293 115.787 0 0 KQK14069 1603 1401.8 1508.76 175.068 KQK14071 474 277.939 7.31705 4.28213 ==> SRR5578516.se.tsv <== BRADI_1g14170v3 1516 BRADI_1g53295v3 20 BRADI_1g59795v3 135 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 555 BRADI_1g74790v3 154 BRADI_1g09890v3 1 BRADI_1g77505v3 138 BRADI_1g48960v3 0 SRR5578516 completed mapping pipeline successfully