Starting /dee2/code/volunteer_pipeline.sh SRR6031157 current disk space = 1523492564992 free memory = 1563784028 SRR6031157 SRAfilesize e20e27de345a34695f1909a7678adeeb SRR6031157.sra SRR6031157.sra file validated SRR6031157 is paired end SRR6031157 is conventional basespace SRR6031157 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031157_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.6325 32.0 25.0 33.0 18.0 34.0 2 31.852 33.0 32.0 33.0 30.0 34.0 3 32.7445 33.0 33.0 33.0 31.0 34.0 4 33.23875 33.0 33.0 34.0 33.0 34.0 5 33.48025 34.0 33.0 34.0 33.0 34.0 6 37.57875 38.0 38.0 38.0 37.0 38.0 7 37.78025 38.0 38.0 38.0 38.0 38.0 8 37.827 38.0 38.0 38.0 38.0 38.0 9 37.769 38.0 38.0 38.0 38.0 38.0 10-14 37.8309 38.0 38.0 38.0 38.0 38.0 15-19 37.81575 38.0 38.0 38.0 38.0 38.0 20-24 37.80665 38.0 38.0 38.0 38.0 38.0 25-29 37.775549999999996 38.0 38.0 38.0 38.0 38.0 30-34 37.7407 38.0 38.0 38.0 38.0 38.0 35-39 37.7351 38.0 38.0 38.0 38.0 38.0 40-44 37.699200000000005 38.0 38.0 38.0 38.0 38.0 45-49 37.6394 38.0 38.0 38.0 38.0 38.0 50-54 37.609899999999996 38.0 38.0 38.0 38.0 38.0 55-59 37.5505 38.0 38.0 38.0 38.0 38.0 60-64 37.576100000000004 38.0 38.0 38.0 38.0 38.0 65-69 37.5101 38.0 38.0 38.0 38.0 38.0 70-74 37.5027 38.0 38.0 38.0 38.0 38.0 75-79 37.4692 38.0 38.0 38.0 38.0 38.0 80-84 37.379400000000004 38.0 38.0 38.0 37.2 38.0 85-89 37.329699999999995 38.0 38.0 38.0 37.0 38.0 90-94 37.30245 38.0 38.0 38.0 37.0 38.0 95-99 37.23365 38.0 38.0 38.0 36.8 38.0 100-104 37.14325 38.0 38.0 38.0 36.4 38.0 105-109 37.0869 38.0 38.0 38.0 36.0 38.0 110-114 36.99475 38.0 38.0 38.0 36.0 38.0 115-119 36.94285 38.0 38.0 38.0 35.4 38.0 120-124 36.77675000000001 38.0 38.0 38.0 35.0 38.0 125-129 36.71220000000001 38.0 38.0 38.0 35.0 38.0 130-134 36.63135 38.0 38.0 38.0 34.8 38.0 135-139 36.4546 38.0 38.0 38.0 34.2 38.0 140-144 36.2254 38.0 38.0 38.0 33.6 38.0 145-149 36.0145 38.0 38.0 38.0 33.4 38.0 150-151 33.357375000000005 37.0 34.5 38.0 17.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 1.0 10 1.0 11 0.0 12 0.0 13 1.0 14 1.0 15 5.0 16 4.0 17 0.0 18 2.0 19 1.0 20 1.0 21 1.0 22 1.0 23 0.0 24 5.0 25 7.0 26 3.0 27 5.0 28 10.0 29 12.0 30 8.0 31 16.0 32 20.0 33 38.0 34 73.0 35 123.0 36 411.0 37 3249.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.06318956870612 12.662988966900702 10.982948846539617 30.29087261785356 2 24.075 14.05 32.725 29.15 3 20.3 21.125 27.975 30.599999999999998 4 22.375 26.375 23.3 27.950000000000003 5 23.875 28.975 25.0 22.15 6 24.55 30.7 24.099999999999998 20.65 7 17.849999999999998 23.275000000000002 38.775 20.1 8 20.724999999999998 24.625 27.224999999999998 27.425 9 18.2 23.075000000000003 32.05 26.674999999999997 10-14 21.060000000000002 27.089999999999996 26.424999999999997 25.424999999999997 15-19 21.745 25.35 27.800000000000004 25.105 20-24 21.595 25.96 27.47 24.975 25-29 21.695 25.7 27.034999999999997 25.569999999999997 30-34 22.17 25.775 26.655 25.4 35-39 22.145 25.345000000000002 26.71 25.8 40-44 21.745 25.75 26.51 25.995 45-49 22.259999999999998 25.52 27.025 25.195 50-54 21.685 25.495 26.779999999999998 26.040000000000003 55-59 22.78 25.4 26.340000000000003 25.480000000000004 60-64 21.505 26.35 26.985 25.16 65-69 21.265 25.619999999999997 27.21 25.905 70-74 21.52 26.529999999999998 26.169999999999998 25.779999999999998 75-79 22.314999999999998 26.16 25.840000000000003 25.685000000000002 80-84 21.48 25.525 27.16 25.835 85-89 21.205 25.86 27.52 25.415 90-94 22.185 26.155 26.484999999999996 25.174999999999997 95-99 21.39 26.064999999999998 26.715 25.83 100-104 21.995 26.75 26.275 24.98 105-109 22.12 25.71 26.375 25.795 110-114 22.615 26.39 26.265 24.73 115-119 22.814999999999998 26.045 26.169999999999998 24.97 120-124 22.39 26.22 26.724999999999998 24.665 125-129 22.295 26.479999999999997 25.900000000000002 25.324999999999996 130-134 22.925 25.89 26.445 24.740000000000002 135-139 21.87 26.3 26.445 25.385 140-144 22.38 26.11 26.11 25.4 145-149 22.595000000000002 25.650000000000002 26.179999999999996 25.575 150-151 22.412499999999998 25.8 26.1 25.687500000000004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 1.0 5 1.5 6 1.0 7 1.0 8 1.0 9 1.5 10 1.0 11 0.0 12 0.5 13 1.0 14 0.5 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 0.5 25 1.5 26 2.0 27 1.5 28 4.0 29 6.5 30 8.5 31 16.5 32 20.5 33 24.0 34 35.0 35 43.0 36 50.0 37 67.5 38 88.0 39 104.5 40 129.5 41 157.5 42 165.0 43 180.0 44 209.0 45 213.0 46 224.0 47 240.5 48 222.0 49 212.5 50 199.5 51 166.0 52 138.5 53 124.5 54 115.0 55 110.0 56 111.0 57 92.0 58 85.0 59 81.5 60 67.0 61 50.5 62 37.5 63 34.0 64 26.5 65 23.0 66 18.5 67 17.5 68 17.0 69 9.5 70 8.0 71 5.0 72 3.0 73 3.5 74 1.5 75 4.5 76 6.0 77 2.5 78 1.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.14999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 93.04542905215928 82.95 2 4.8233314638250135 8.6 3 1.0095344924284912 2.7 4 0.4206393718452047 1.5 5 0.2523836231071228 1.125 6 0.11217049915872125 0.6 7 0.1962983735277622 1.225 8 0.028042624789680313 0.2 9 0.0 0.0 >10 0.11217049915872125 1.0999999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 12 0.3 No Hit ACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACG 11 0.27499999999999997 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 11 0.27499999999999997 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 10 0.25 No Hit GTTCAGTCATAATCCGGCACACGGTAGCTTCGCGCCACTGGCTTTTCAAC 8 0.2 No Hit GTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTT 7 0.17500000000000002 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 7 0.17500000000000002 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 7 0.17500000000000002 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 7 0.17500000000000002 No Hit GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA 7 0.17500000000000002 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 7 0.17500000000000002 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 7 0.17500000000000002 No Hit GCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCA 6 0.15 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 6 0.15 No Hit GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC 6 0.15 No Hit CTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACGTCGG 6 0.15 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA 5 0.125 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 5 0.125 No Hit GGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTA 5 0.125 No Hit GTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTAT 5 0.125 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 5 0.125 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 5 0.125 No Hit CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC 5 0.125 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 5 0.125 No Hit CCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1 0.0 0.0 0.0 0.0 110-111 0.1 0.0 0.0 0.0 0.0 112-113 0.1 0.0 0.0 0.0 0.0 114-115 0.1 0.0 0.0 0.0 0.0 116-117 0.1 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.1125 0.0 0.0 0.0 0.0 124-125 0.1375 0.0 0.0 0.0 0.0 126-127 0.175 0.0 0.0 0.0 0.0 128-129 0.1875 0.0 0.0 0.0 0.0 130-131 0.25 0.0 0.0 0.0 0.0 132-133 0.2875 0.0 0.0 0.0 0.0 134-135 0.35 0.0 0.0 0.0 0.0 136-137 0.4 0.0 0.0 0.0 0.0 138-139 0.4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGTCA 20 0.00593511 29.0 110-114 >>END_MODULE SRR6031157 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031157_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68425 34.0 33.0 34.0 33.0 34.0 2 32.81825 34.0 33.0 34.0 33.0 34.0 3 32.87175 34.0 33.0 34.0 33.0 34.0 4 32.824 34.0 33.0 34.0 33.0 34.0 5 32.88375 34.0 33.0 34.0 33.0 34.0 6 36.85375 38.0 38.0 38.0 38.0 38.0 7 36.8925 38.0 38.0 38.0 38.0 38.0 8 36.928 38.0 38.0 38.0 38.0 38.0 9 36.9145 38.0 38.0 38.0 38.0 38.0 10-14 36.864200000000004 38.0 38.0 38.0 38.0 38.0 15-19 36.7534 38.0 38.0 38.0 38.0 38.0 20-24 36.7403 38.0 38.0 38.0 38.0 38.0 25-29 36.7884 38.0 38.0 38.0 38.0 38.0 30-34 37.0055 38.0 38.0 38.0 38.0 38.0 35-39 37.05545 38.0 38.0 38.0 38.0 38.0 40-44 37.02955 38.0 38.0 38.0 38.0 38.0 45-49 37.044 38.0 38.0 38.0 38.0 38.0 50-54 36.995799999999996 38.0 38.0 38.0 38.0 38.0 55-59 36.8365 38.0 38.0 38.0 38.0 38.0 60-64 36.8632 38.0 38.0 38.0 37.4 38.0 65-69 36.792899999999996 38.0 38.0 38.0 37.0 38.0 70-74 36.6231 38.0 38.0 38.0 37.2 38.0 75-79 36.67995 38.0 38.0 38.0 37.0 38.0 80-84 36.802749999999996 38.0 38.0 38.0 37.0 38.0 85-89 36.7826 38.0 38.0 38.0 37.0 38.0 90-94 36.7427 38.0 38.0 38.0 36.6 38.0 95-99 36.6933 38.0 38.0 38.0 36.2 38.0 100-104 36.5359 38.0 38.0 38.0 36.0 38.0 105-109 36.38745 38.0 38.0 38.0 36.0 38.0 110-114 36.33625 38.0 38.0 38.0 35.2 38.0 115-119 36.18755 38.0 38.0 38.0 35.0 38.0 120-124 36.140750000000004 38.0 38.0 38.0 34.8 38.0 125-129 36.2294 38.0 38.0 38.0 34.8 38.0 130-134 36.11015 38.0 38.0 38.0 34.4 38.0 135-139 36.0082 38.0 38.0 38.0 34.0 38.0 140-144 35.8317 38.0 38.0 38.0 33.6 38.0 145-149 35.4643 38.0 37.8 38.0 32.6 38.0 150-151 32.536125 37.0 33.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58.0 3 2.0 4 1.0 5 1.0 6 1.0 7 0.0 8 1.0 9 1.0 10 1.0 11 1.0 12 1.0 13 2.0 14 2.0 15 4.0 16 4.0 17 3.0 18 4.0 19 1.0 20 4.0 21 1.0 22 6.0 23 4.0 24 5.0 25 11.0 26 8.0 27 12.0 28 16.0 29 9.0 30 15.0 31 28.0 32 35.0 33 45.0 34 58.0 35 108.0 36 288.0 37 3259.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.82077393075356 20.74847250509165 12.24541751527495 24.185336048879837 2 29.987293519695047 26.683608640406607 24.904701397712834 18.424396442185515 3 23.65482233502538 26.979695431472084 28.527918781725887 20.83756345177665 4 25.895806861499366 32.630241423125796 20.025412960609913 21.448538754764932 5 28.45177664974619 32.61421319796954 19.16243654822335 19.77157360406091 6 24.40824637312293 35.09798931025706 19.82692797149402 20.666836345125986 7 22.618441161487517 20.453387671930717 33.97860417727968 22.949566989302088 8 24.85372678707708 23.785296362248793 23.02213177308573 28.3388450775884 9 23.12579415501906 24.650571791613725 27.445997458703943 24.777636594663278 10-14 25.375337167285867 27.120973077510307 24.006310753727924 23.497379001475903 15-19 25.140291806958476 25.762677277828793 26.124885215794308 22.972145699418427 20-24 25.997143731510764 26.109354279302256 25.39018667754769 22.503315311639295 25-29 25.444631300005092 26.88681649085257 25.12357947306732 22.544972736075014 30-34 25.74543610547667 27.19066937119676 25.096348884381335 21.967545638945232 35-39 25.784889609074334 27.223009925055702 24.680980352440756 22.311120113429208 40-44 26.254552812626468 26.12302711452853 24.620598947794413 23.001821125050586 45-49 25.781526185546184 26.18049593454876 25.357305186606737 22.680672693298316 50-54 25.38430420711974 26.25910194174757 25.313511326860844 23.043082524271842 55-59 25.386571719226858 25.61546286876907 25.879959308240082 23.11800610376399 60-64 25.865745912460646 26.089164212450495 25.43922006702549 22.60586980806337 65-69 26.025725761350348 26.010473333672273 25.268188520006102 22.695612384971277 70-74 26.129295401244008 26.231263383297645 25.165697970837158 22.47374324462119 75-79 25.857600243939626 25.75087665802714 25.09528891599329 23.296234182039946 80-84 25.50078207780413 26.212220596397394 25.13749432362884 23.149503002169634 85-89 25.917338709677416 25.41834677419355 25.68548387096774 22.978830645161292 90-94 25.937673060464178 26.139052509691385 25.761466042390374 22.16180838745406 95-99 26.272984441301272 25.585976965043443 26.091129521115374 22.049909072539908 100-104 25.36912070627632 25.60251661677406 26.62235526916637 22.406007407783246 105-109 26.189627970889102 26.10310957300626 25.797750521655043 21.90951193444959 110-114 26.24471283697702 26.23452071548693 25.138867655302448 22.381898792233603 115-119 26.291607106391666 26.363079436389626 24.606902185011233 22.738411272207472 120-124 25.973232914355503 26.039387308533918 25.220090580632025 22.76728919647855 125-129 25.56737588652482 26.372847011144884 25.49645390070922 22.563323201621074 130-134 25.668989195193376 26.062809249722307 25.759870746238512 22.508330808845805 135-139 25.37366010769463 27.01424185999698 25.262946001711033 22.349152030597352 140-144 25.974548563955537 26.381972737789848 25.064131582918364 22.57934711533625 145-149 25.326633165829143 26.507537688442213 25.42713567839196 22.738693467336685 150-151 25.454545454545453 25.643939393939398 25.757575757575758 23.143939393939394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 13.0 2 0.5 3 2.0 4 2.0 5 0.5 6 1.0 7 1.5 8 3.0 9 2.5 10 1.5 11 1.5 12 2.0 13 3.0 14 1.5 15 0.0 16 1.5 17 2.5 18 2.0 19 2.0 20 2.0 21 3.0 22 2.0 23 1.0 24 2.0 25 2.0 26 2.0 27 3.5 28 3.5 29 6.0 30 9.0 31 10.0 32 12.5 33 22.0 34 35.5 35 48.0 36 67.0 37 79.5 38 87.5 39 112.5 40 143.5 41 158.5 42 168.0 43 187.0 44 184.0 45 163.0 46 171.5 47 209.5 48 207.5 49 172.5 50 164.0 51 162.0 52 145.0 53 135.5 54 125.5 55 110.5 56 89.0 57 61.0 58 65.0 59 69.0 60 56.0 61 42.5 62 40.0 63 43.0 64 43.5 65 43.5 66 42.0 67 54.0 68 54.5 69 39.0 70 23.5 71 13.0 72 12.0 73 7.5 74 6.0 75 7.0 76 4.5 77 4.0 78 4.5 79 2.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7999999999999998 2 1.625 3 1.5 4 1.625 5 1.5 6 1.775 7 1.8499999999999999 8 1.725 9 1.625 10-14 1.755 15-19 1.9900000000000002 20-24 1.97 25-29 1.8849999999999998 30-34 1.4000000000000001 35-39 1.26 40-44 1.16 45-49 0.9950000000000001 50-54 1.1199999999999999 55-59 1.7000000000000002 60-64 1.53 65-69 1.6549999999999998 70-74 1.9300000000000002 75-79 1.6150000000000002 80-84 0.905 85-89 0.8 90-94 0.685 95-99 1.02 100-104 1.455 105-109 1.755 110-114 1.8849999999999998 115-119 2.06 120-124 1.745 125-129 1.3 130-134 0.97 135-139 0.645 140-144 0.5950000000000001 145-149 0.5 150-151 1.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.97500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 92.4410343847684 81.325 2 5.029838022165388 8.85 3 1.4208581983518045 3.75 4 0.4262574595055414 1.5 5 0.2557544757033248 1.125 6 0.08525149190110827 0.44999999999999996 7 0.08525149190110827 0.525 8 0.11366865586814436 0.8 9 0.05683432793407218 0.44999999999999996 >10 0.08525149190110827 1.225 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 27 0.675 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 12 0.3 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 10 0.25 No Hit AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGA 9 0.22499999999999998 No Hit GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGGGTCGGAA 9 0.22499999999999998 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 8 0.2 No Hit CTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCG 8 0.2 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 8 0.2 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 8 0.2 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 7 0.17500000000000002 No Hit AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA 7 0.17500000000000002 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 7 0.17500000000000002 No Hit GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC 6 0.15 No Hit GAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTT 6 0.15 No Hit GCCAGAGGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCG 6 0.15 No Hit AAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTT 5 0.125 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 5 0.125 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 5 0.125 No Hit AGAAATTAGAGTGCTCAAAGCAAGCCATCGCTCTGGATACATTAGCATGG 5 0.125 No Hit GGCAAGTCCCCTCCTTTTGGCTCCAAGGCCCGGTCTGACCGGGCCGATCC 5 0.125 No Hit GGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTC 5 0.125 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 5 0.125 No Hit GGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTT 5 0.125 No Hit GGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1 0.0 0.0 0.0 0.0 110-111 0.1 0.0 0.0 0.0 0.0 112-113 0.1 0.0 0.0 0.0 0.0 114-115 0.1 0.0 0.0 0.0 0.0 116-117 0.1 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.1125 0.0 0.0 0.0 0.0 124-125 0.1375 0.0 0.0 0.0 0.0 126-127 0.2 0.0 0.0 0.0 0.0 128-129 0.21250000000000002 0.0 0.0 0.0 0.0 130-131 0.275 0.0 0.0 0.0 0.0 132-133 0.3125 0.0 0.0 0.0 0.0 134-135 0.375 0.0 0.0 0.0 0.0 136-137 0.4375 0.0 0.0 0.0 0.0 138-139 0.45 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554834 spots for SRR6031157.sra Written 554834 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra Read 554826 spots for SRR6031157.sra Written 554826 spots for SRR6031157.sra SRR ids: ['SRR6031157.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_av4j__4n SRR6031157.sra spots: 11096528 blocks: [[1, 554826], [554827, 1109652], [1109653, 1664478], [1664479, 2219304], [2219305, 2774130], [2774131, 3328956], [3328957, 3883782], [3883783, 4438608], [4438609, 4993434], [4993435, 5548260], [5548261, 6103086], [6103087, 6657912], [6657913, 7212738], [7212739, 7767564], [7767565, 8322390], [8322391, 8877216], [8877217, 9432042], [9432043, 9986868], [9986869, 10541694], [10541695, 11096528]] SRR6031157 file size 3738548 SRR6031157 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6031157 SRR6031157_1.fastq SRR6031157_2.fastq Input file: SRR6031157_1.fastq Paired file: SRR6031157_2.fastq trimmed: SRR6031157-trimmed-pair1.fastq, SRR6031157-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 00:18:37 2024 >> started Tue Dec 10 00:18:48 2024 >> done (11.224s) 11096528 read pairs processed; of these: 8781 ( 0.08%) short read pairs filtered out after trimming by size control 16280 ( 0.15%) empty read pairs filtered out after trimming by size control 11071467 (99.77%) read pairs available; of these: 3110533 (28.10%) trimmed read pairs available after processing 7960934 (71.90%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 4 0.00% 20 5 0.00% 21 4 0.00% 22 7 0.00% 23 4 0.00% 24 7 0.00% 25 8 0.00% 26 3 0.00% 27 5 0.00% 28 4 0.00% 29 5 0.00% 30 7 0.00% 31 9 0.00% 32 2 0.00% 33 4 0.00% 34 3 0.00% 35 3 0.00% 36 4 0.00% 37 11 0.00% 38 10 0.00% 39 3 0.00% 40 7 0.00% 41 4 0.00% 42 9 0.00% 43 5 0.00% 44 8 0.00% 45 11 0.00% 46 16 0.00% 47 14 0.00% 48 13 0.00% 49 9 0.00% 50 16 0.00% 51 18 0.00% 52 31 0.00% 53 24 0.00% 54 18 0.00% 55 26 0.00% 56 25 0.00% 57 34 0.00% 58 40 0.00% 59 44 0.00% 60 46 0.00% 61 48 0.00% 62 55 0.00% 63 61 0.00% 64 56 0.00% 65 69 0.00% 66 80 0.00% 67 81 0.00% 68 107 0.00% 69 121 0.00% 70 102 0.00% 71 139 0.00% 72 147 0.00% 73 133 0.00% 74 180 0.00% 75 172 0.00% 76 211 0.00% 77 259 0.00% 78 232 0.00% 79 298 0.00% 80 324 0.00% 81 327 0.00% 82 398 0.00% 83 440 0.00% 84 811 0.01% 85 1138 0.01% 86 1231 0.01% 87 1385 0.01% 88 1591 0.01% 89 1557 0.01% 90 1647 0.01% 91 1498 0.01% 92 1493 0.01% 93 1418 0.01% 94 1454 0.01% 95 1542 0.01% 96 1446 0.01% 97 1442 0.01% 98 1510 0.01% 99 1555 0.01% 100 1704 0.02% 101 1774 0.02% 102 1791 0.02% 103 1797 0.02% 104 1994 0.02% 105 2684 0.02% 106 2348 0.02% 107 2134 0.02% 108 2566 0.02% 109 2725 0.02% 110 2947 0.03% 111 3107 0.03% 112 3503 0.03% 113 3903 0.04% 114 4951 0.04% 115 5757 0.05% 116 5983 0.05% 117 5365 0.05% 118 4581 0.04% 119 4264 0.04% 120 4517 0.04% 121 4166 0.04% 122 4345 0.04% 123 4824 0.04% 124 4808 0.04% 125 5285 0.05% 126 5237 0.05% 127 5581 0.05% 128 5781 0.05% 129 6055 0.05% 130 6488 0.06% 131 6957 0.06% 132 7274 0.07% 133 8050 0.07% 134 8564 0.08% 135 9014 0.08% 136 9788 0.09% 137 10655 0.10% 138 11766 0.11% 139 12598 0.11% 140 13860 0.13% 141 16241 0.15% 142 18128 0.16% 143 21032 0.19% 144 26797 0.24% 145 38289 0.35% 146 63691 0.58% 147 68871 0.62% 148 126900 1.15% 149 309237 2.79% 150 2172565 19.62% 151 7960934 71.90% 11071467 reads passed initial QC criterion=sequence-density sequence-density=2.46 sequence-density-rank=1 fanout-score=2.50 fanout-score-rank=25 prefix-density=2.56 prefix-fanout=2.4 sequence=CCCAGCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGATCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTTGCCCCGCTTCCGACCCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC criterion=fanout-score sequence-density=0.03 sequence-density-rank=26 fanout-score=101.29 fanout-score-rank=1 prefix-density=2.72 prefix-fanout=1.0 sequence=GTCTCACGACGTTCTAAACCCAACTCACGTACCACTTTCATCGGCGAACAACCGAACCCTTGGGACCTTCTTCAACCCCAGGATGTGATGAGTCGACATCGAGGTGCCAAACGACTCCGTCGATAAGAGCTCTTGGGAGTCATCAGCCTGTTATCCCCGGCGTACCTTTGATCCGTTGAGCGAGAGCCCTTCCACACGGGACTCCCGGATCACTATGGCCGACTTTCGTCTCTGTTCGACCAGTCGGTCTCACAGTCAGGCAGGCTTATACCATTACGCTCACGAGCAGAATCAACGCTTGAGCCTACCTTCGCACACCTCCGTTACTCTTTAGGAGGCATCCGCCCCAGATAAACTACCCACCTCGCAGTGTCCCGCCCCCCCCGAATGATCGGTGCGGCGGTTAGGCATCCTTAGACGAAAGAGTGGTCTTTCAGGATTGGTCGTTGTGTGTCACCACCTCCCACCTATCCTACACATTCGATCAAGGTTGTCACTGCGAAGCTATAGT criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.57 fanout-score-rank=24 prefix-density=0.92 prefix-fanout=1.0 sequence=GAGAAACGGCTGCCACATCCAAG criterion=fanout-score sequence-density=0.05 sequence-density-rank=21 fanout-score=138.10 fanout-score-rank=1 prefix-density=0.42 prefix-fanout=15.7 sequence=GAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATTGTTGTTCTCCTGCTCATCGTGCTTTCGGTTTGTGCCGCAGGAGGAAGGGAGCTGGCGGAACAAAAGCTACAAAAGGACTTCTACAGTGCTGCATCCAAAGAGGGAGCAACAGTTTCGAGCAACCATCCAAGGAACCTCATGGTTAAGACGAACGACTACGGCCGCTACGACCCTGCTCCAG SRR6031157 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 00:19:43 Started mapping on | Dec 10 00:19:43 Finished on | Dec 10 00:22:06 Mapping speed, Million of reads per hour | 278.72 Number of input reads | 11071467 Average input read length | 300 UNIQUE READS: Uniquely mapped reads number | 6725394 Uniquely mapped reads % | 60.75% Average mapped length | 299.50 Number of splices: Total | 7726690 Number of splices: Annotated (sjdb) | 7366089 Number of splices: GT/AG | 7628419 Number of splices: GC/AG | 87693 Number of splices: AT/AC | 5469 Number of splices: Non-canonical | 5109 Mismatch rate per base, % | 0.17% Deletion rate per base | 0.00% Deletion average length | 1.39 Insertion rate per base | 0.00% Insertion average length | 1.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 82667 % of reads mapped to multiple loci | 0.75% Number of reads mapped to too many loci | 310058 % of reads mapped to too many loci | 2.80% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.13% % of reads unmapped: other | 28.57% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4267882 4267882 4267882 N_multimapping 82667 82667 82667 N_noFeature 298342 6543359 347733 N_ambiguous 165527 1558 32965 UnstrandedReadsAssigned:6261525 PositiveStrandReadsAssigned:180477 NegativeStrandReadsAssigned:6344696 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6031157 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6031157-trimmed-pair1.fastq SRR6031157-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 11,071,467 reads, 6,578,336 reads pseudoaligned [quant] estimated average fragment length: 464.442 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,126 rounds 52973 SRR6031157.ke.tsv 35125 SRR6031157.se.tsv 88098 total ==> SRR6031157.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 474.154 0 0 PNS24247 1044 580.558 36.9634 13.7209 PNS24249 1928 1464.56 10.5714 1.55555 PNS24246 1044 580.558 36.9634 13.7209 PNS24248 1044 580.558 36.9634 13.7209 PNS24244 1471 1007.56 21.5384 4.60682 PNS24243 293 63.3503 0 0 KQK14069 1603 1139.56 958.575 181.279 KQK14071 474 116.839 4.45889 8.22425 ==> SRR6031157.se.tsv <== BRADI_1g14170v3 960 BRADI_1g53295v3 53 BRADI_1g59795v3 239 BRADI_1g07683v3 0 BRADI_1g00485v3 42 BRADI_1g20270v3 252 BRADI_1g74790v3 82 BRADI_1g09890v3 0 BRADI_1g77505v3 86 BRADI_1g48960v3 0 SRR6031157 completed mapping pipeline successfully