Starting /dee2/code/volunteer_pipeline.sh SRR6031160 current disk space = 1523578695680 free memory = 1567325560 SRR6031160 SRAfilesize 81d7999093c69ffee4c8aa2082fbafe2 SRR6031160.sra SRR6031160.sra file validated SRR6031160 is paired end SRR6031160 is conventional basespace SRR6031160 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031160_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4805 32.0 31.0 33.0 30.0 33.0 2 32.8985 33.0 33.0 33.0 33.0 34.0 3 33.14225 33.0 33.0 34.0 33.0 34.0 4 33.49275 34.0 33.0 34.0 33.0 34.0 5 33.609 34.0 33.0 34.0 33.0 34.0 6 37.2915 38.0 38.0 38.0 37.0 38.0 7 37.74525 38.0 38.0 38.0 38.0 38.0 8 37.754 38.0 38.0 38.0 38.0 38.0 9 37.86575 38.0 38.0 38.0 38.0 38.0 10-14 37.857150000000004 38.0 38.0 38.0 38.0 38.0 15-19 37.8572 38.0 38.0 38.0 38.0 38.0 20-24 37.87265 38.0 38.0 38.0 38.0 38.0 25-29 37.841100000000004 38.0 38.0 38.0 38.0 38.0 30-34 37.832499999999996 38.0 38.0 38.0 38.0 38.0 35-39 37.83885 38.0 38.0 38.0 38.0 38.0 40-44 37.819 38.0 38.0 38.0 38.0 38.0 45-49 37.79065000000001 38.0 38.0 38.0 38.0 38.0 50-54 37.7358 38.0 38.0 38.0 38.0 38.0 55-59 37.73045 38.0 38.0 38.0 38.0 38.0 60-64 37.72709999999999 38.0 38.0 38.0 38.0 38.0 65-69 37.7215 38.0 38.0 38.0 38.0 38.0 70-74 37.66615 38.0 38.0 38.0 38.0 38.0 75-79 37.64755 38.0 38.0 38.0 38.0 38.0 80-84 37.64555 38.0 38.0 38.0 38.0 38.0 85-89 37.602599999999995 38.0 38.0 38.0 38.0 38.0 90-94 37.54545 38.0 38.0 38.0 38.0 38.0 95-99 37.51125 38.0 38.0 38.0 37.6 38.0 100-104 37.463 38.0 38.0 38.0 37.6 38.0 105-109 37.4351 38.0 38.0 38.0 37.4 38.0 110-114 37.396249999999995 38.0 38.0 38.0 37.0 38.0 115-119 37.34075 38.0 38.0 38.0 36.6 38.0 120-124 37.212900000000005 38.0 38.0 38.0 36.2 38.0 125-129 37.1486 38.0 38.0 38.0 36.0 38.0 130-134 37.017700000000005 38.0 38.0 38.0 35.6 38.0 135-139 37.045 38.0 38.0 38.0 35.6 38.0 140-144 36.86105 38.0 38.0 38.0 35.0 38.0 145-149 36.659800000000004 38.0 38.0 38.0 34.8 38.0 150-151 34.456 38.0 35.5 38.0 28.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 2.0 19 2.0 20 0.0 21 1.0 22 0.0 23 2.0 24 0.0 25 2.0 26 1.0 27 3.0 28 4.0 29 5.0 30 10.0 31 14.0 32 15.0 33 29.0 34 41.0 35 87.0 36 270.0 37 3510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.997241033358414 10.609480812641085 4.5899172310007526 31.80336092299975 2 21.575 10.025 36.25 32.15 3 17.625 15.15 27.224999999999998 40.0 4 23.175 22.35 24.099999999999998 30.375000000000004 5 25.55 24.45 24.375 25.624999999999996 6 25.075681130171546 29.56609485368315 23.738647830474267 21.61957618567104 7 16.7 26.174999999999997 38.35 18.775 8 19.275000000000002 22.925 30.5 27.3 9 19.0 21.55 33.725 25.724999999999998 10-14 22.009999999999998 26.985 26.05 24.955 15-19 22.945 24.84 27.0 25.215 20-24 22.375 26.224999999999998 26.875 24.525 25-29 21.62 25.490000000000002 26.740000000000002 26.150000000000002 30-34 21.87 25.074999999999996 26.045 27.01 35-39 22.175 25.495 26.565 25.765 40-44 22.770000000000003 25.105 26.445 25.679999999999996 45-49 21.771088554427724 24.71623581179059 26.736336816840844 26.776338816940846 50-54 21.44107205360268 25.721286064303218 26.3713185659283 26.466323316165806 55-59 22.245 24.955 26.369999999999997 26.43 60-64 21.94 25.09 27.155 25.814999999999998 65-69 22.285 24.060000000000002 27.05 26.605 70-74 22.065 25.45 26.415 26.07 75-79 22.46 25.509999999999998 25.88 26.150000000000002 80-84 21.825 25.240000000000002 26.650000000000002 26.284999999999997 85-89 22.535 25.28 26.375 25.81 90-94 21.525 25.865 26.705000000000002 25.905 95-99 21.625 24.709999999999997 26.979999999999997 26.685 100-104 21.959999999999997 24.94 26.435 26.665 105-109 22.34 25.080000000000002 26.96 25.619999999999997 110-114 22.34 25.900000000000002 26.415 25.345000000000002 115-119 22.48 25.235000000000003 26.33 25.955000000000002 120-124 22.96 25.095 26.665 25.28 125-129 22.335 25.319999999999997 25.619999999999997 26.724999999999998 130-134 22.915 24.52 26.015 26.55 135-139 22.314999999999998 25.005 26.215 26.465 140-144 23.2061603080154 25.156257812890644 25.351267563378173 26.286314315715785 145-149 23.150000000000002 24.995 26.105 25.75 150-151 22.1055263815954 26.531632908227053 24.868717179294826 26.494123530882717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 1.0 23 0.5 24 0.5 25 1.5 26 2.0 27 2.0 28 6.5 29 7.0 30 10.5 31 13.0 32 12.0 33 15.5 34 21.0 35 31.5 36 48.5 37 67.5 38 92.0 39 103.5 40 105.5 41 119.0 42 135.0 43 158.0 44 179.5 45 185.0 46 187.0 47 220.0 48 222.5 49 205.5 50 193.5 51 174.5 52 174.5 53 170.5 54 155.0 55 151.0 56 146.5 57 116.5 58 104.0 59 100.0 60 86.0 61 58.0 62 37.0 63 40.0 64 29.5 65 25.5 66 24.5 67 15.5 68 12.0 69 11.0 70 8.5 71 4.0 72 3.0 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.8999999999999999 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.005 50-54 0.005 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.0 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.325 #Duplication Level Percentage of deduplicated Percentage of total 1 93.32012454005095 82.425 2 3.9343334276818567 6.950000000000001 3 1.4435324087178034 3.8249999999999997 4 0.5660911406736484 2.0 5 0.22643645626945935 1.0 6 0.16982734220209456 0.8999999999999999 7 0.1415227851684121 0.8750000000000001 8 0.05660911406736484 0.4 9 0.0 0.0 >10 0.1415227851684121 1.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 19 0.475 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 13 0.325 No Hit GTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA 12 0.3 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 11 0.27499999999999997 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 10 0.25 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 8 0.2 No Hit GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 8 0.2 No Hit GTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC 7 0.17500000000000002 No Hit CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT 7 0.17500000000000002 No Hit CCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCT 7 0.17500000000000002 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 7 0.17500000000000002 No Hit GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT 7 0.17500000000000002 No Hit GTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTT 6 0.15 No Hit CTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTC 6 0.15 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 6 0.15 No Hit GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT 6 0.15 No Hit GTGACAATCGCCCTATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTC 6 0.15 No Hit GTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCT 6 0.15 No Hit GGTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGA 5 0.125 No Hit GGCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTC 5 0.125 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 5 0.125 No Hit GTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCAC 5 0.125 No Hit ACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACG 5 0.125 No Hit GTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTT 5 0.125 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 5 0.125 No Hit GGCACGGTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0125 0.0 0.0 0.0 66-67 0.0 0.025 0.0 0.0 0.0 68-69 0.0 0.025 0.0 0.0 0.0 70-71 0.0 0.025 0.0 0.0 0.0 72-73 0.0 0.025 0.0 0.0 0.0 74-75 0.0 0.025 0.0 0.0 0.0 76-77 0.0 0.025 0.0 0.0 0.0 78-79 0.0 0.025 0.0 0.0 0.0 80-81 0.0 0.025 0.0 0.0 0.0 82-83 0.0 0.025 0.0 0.0 0.0 84-85 0.0 0.025 0.0 0.0 0.0 86-87 0.025 0.025 0.0 0.0 0.0 88-89 0.025 0.025 0.0 0.0 0.0 90-91 0.05 0.025 0.0 0.0 0.0 92-93 0.075 0.025 0.0 0.0 0.0 94-95 0.1 0.025 0.0 0.0 0.0 96-97 0.1125 0.025 0.0 0.0 0.0 98-99 0.15 0.025 0.0 0.0 0.0 100-101 0.15 0.025 0.0 0.0 0.0 102-103 0.175 0.025 0.0 0.0 0.0 104-105 0.2 0.025 0.0 0.0 0.0 106-107 0.21250000000000002 0.025 0.0 0.0 0.0 108-109 0.225 0.025 0.0 0.0 0.0 110-111 0.2625 0.025 0.0 0.0 0.0 112-113 0.275 0.025 0.0 0.0 0.0 114-115 0.275 0.025 0.0 0.0 0.0 116-117 0.3 0.025 0.0 0.0 0.0 118-119 0.3125 0.025 0.0 0.0 0.0 120-121 0.3625 0.025 0.0 0.0 0.0 122-123 0.4375 0.025 0.0 0.0 0.0 124-125 0.5125 0.025 0.0 0.0 0.0 126-127 0.5375000000000001 0.025 0.0 0.0 0.0 128-129 0.55 0.025 0.0 0.0 0.0 130-131 0.55 0.025 0.0 0.0 0.0 132-133 0.55 0.025 0.0 0.0 0.0 134-135 0.55 0.025 0.0 0.0 0.0 136-137 0.625 0.025 0.0 0.0 0.0 138-139 0.6875 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCAGCA 10 0.006841402 144.925 145 >>END_MODULE SRR6031160 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031160_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.4235 34.0 33.0 34.0 33.0 34.0 2 33.44075 34.0 33.0 34.0 33.0 34.0 3 33.32825 34.0 33.0 34.0 33.0 34.0 4 33.3045 34.0 33.0 34.0 33.0 34.0 5 33.33775 34.0 33.0 34.0 33.0 34.0 6 37.457 38.0 38.0 38.0 38.0 38.0 7 37.49825 38.0 38.0 38.0 38.0 38.0 8 37.5045 38.0 38.0 38.0 38.0 38.0 9 37.56025 38.0 38.0 38.0 38.0 38.0 10-14 37.45 38.0 38.0 38.0 38.0 38.0 15-19 37.3253 38.0 38.0 38.0 38.0 38.0 20-24 37.12425 38.0 38.0 38.0 38.0 38.0 25-29 37.313849999999995 38.0 38.0 38.0 38.0 38.0 30-34 37.618 38.0 38.0 38.0 38.0 38.0 35-39 37.63595 38.0 38.0 38.0 38.0 38.0 40-44 37.600550000000005 38.0 38.0 38.0 38.0 38.0 45-49 37.5997 38.0 38.0 38.0 38.0 38.0 50-54 37.5629 38.0 38.0 38.0 38.0 38.0 55-59 37.42775 38.0 38.0 38.0 38.0 38.0 60-64 37.5346 38.0 38.0 38.0 38.0 38.0 65-69 37.5323 38.0 38.0 38.0 38.0 38.0 70-74 37.3698 38.0 38.0 38.0 38.0 38.0 75-79 37.49895 38.0 38.0 38.0 38.0 38.0 80-84 37.483999999999995 38.0 38.0 38.0 38.0 38.0 85-89 37.40615 38.0 38.0 38.0 38.0 38.0 90-94 37.353 38.0 38.0 38.0 38.0 38.0 95-99 37.34765 38.0 38.0 38.0 38.0 38.0 100-104 37.31595 38.0 38.0 38.0 38.0 38.0 105-109 37.1374 38.0 38.0 38.0 37.8 38.0 110-114 37.0079 38.0 38.0 38.0 37.4 38.0 115-119 36.807100000000005 38.0 38.0 38.0 36.2 38.0 120-124 36.87555 38.0 38.0 38.0 36.0 38.0 125-129 36.99705 38.0 38.0 38.0 36.0 38.0 130-134 36.96755 38.0 38.0 38.0 36.0 38.0 135-139 36.94535 38.0 38.0 38.0 36.0 38.0 140-144 36.817099999999996 38.0 38.0 38.0 35.6 38.0 145-149 36.6435 38.0 38.0 38.0 35.0 38.0 150-151 34.3995 37.0 35.5 38.0 28.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 2.0 4 2.0 5 0.0 6 1.0 7 1.0 8 1.0 9 0.0 10 3.0 11 1.0 12 0.0 13 3.0 14 0.0 15 1.0 16 1.0 17 0.0 18 1.0 19 1.0 20 1.0 21 3.0 22 3.0 23 8.0 24 8.0 25 5.0 26 3.0 27 11.0 28 6.0 29 12.0 30 15.0 31 22.0 32 27.0 33 22.0 34 46.0 35 61.0 36 197.0 37 3525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.087021755438855 21.405351337834457 6.976744186046512 23.53088272068017 2 26.476476476476474 27.902902902902905 26.5015015015015 19.11911911911912 3 23.273914135074065 26.487572181772535 28.119507908611602 22.119005774541804 4 27.875439477649422 31.265695630336516 20.190858864892014 20.66800602712205 5 28.707653701380174 32.94855708908406 19.87452948557089 18.469259723964868 6 23.933768188660313 36.001003512293025 19.518314099347716 20.546914199698946 7 21.675865529352734 23.15604616156548 34.169593577521326 20.99849473156046 8 25.244667503136764 22.710163111668756 26.02258469259724 26.02258469259724 9 25.306940616386868 23.62816336757705 26.98571786519669 24.07917815083939 10-14 26.48876827981306 27.227498869289914 23.58409970350269 22.69963314739434 15-19 26.232234653764742 25.53170043342405 25.385545811914124 22.85051910089709 20-24 25.86268051684824 26.648087154801114 24.66683557131999 22.822396757030656 25-29 26.32824696580551 26.17716674220678 24.948380923603768 22.546205368383944 30-34 25.635 26.924999999999997 25.155 22.285 35-39 26.064999999999998 27.42 24.195 22.32 40-44 26.275 26.205000000000002 25.31 22.21 45-49 25.745 26.784999999999997 25.115 22.355 50-54 25.735000000000003 26.995 24.795 22.475 55-59 26.071034413564764 26.09611718671616 25.634594160730412 22.198254238988664 60-64 26.979999999999997 25.645 25.324999999999996 22.05 65-69 27.17674139311449 25.715572457966374 24.744795836669336 22.3628903122498 70-74 26.766433987847133 25.531060111484962 25.450710591071157 22.251795309596744 75-79 26.705000000000002 25.180000000000003 24.740000000000002 23.375 80-84 26.889999999999997 26.32 25.0 21.790000000000003 85-89 26.687668766876687 25.052505250525055 25.11751175117512 23.142314231423143 90-94 26.845000000000002 25.86 24.795 22.5 95-99 26.4063203160158 25.576278813940696 24.866243312165608 23.151157557877895 100-104 25.97759775977598 26.227622762276226 25.192519251925194 22.6022602260226 105-109 25.605466787257562 25.675811476233545 26.836498844337253 21.88222289217164 110-114 25.861547762998793 26.350261991132605 25.90689238210399 21.881297863764612 115-119 26.470439743525016 26.127126773362953 25.268844347957792 22.13358913515424 120-124 26.57325096680227 25.970569032193257 24.57937823313746 22.876801767867008 125-129 26.36 26.345000000000002 24.2 23.095 130-134 26.640000000000004 26.900000000000002 23.945 22.515 135-139 26.565 25.72 25.34 22.375 140-144 26.595000000000002 26.16 24.88 22.365 145-149 25.925185037007402 26.805361072214446 24.77995599119824 22.489497899579916 150-151 26.469117279319832 27.581895473868467 23.830957739434858 22.118029507376843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 0.5 23 0.5 24 1.0 25 2.0 26 3.5 27 4.0 28 7.0 29 11.0 30 13.5 31 13.5 32 16.5 33 21.0 34 28.5 35 44.0 36 56.5 37 69.5 38 78.0 39 96.5 40 129.5 41 146.5 42 149.0 43 156.0 44 174.0 45 182.5 46 176.5 47 180.5 48 188.5 49 191.0 50 165.0 51 149.0 52 147.5 53 159.0 54 164.5 55 157.0 56 131.5 57 91.0 58 88.0 59 88.0 60 70.5 61 52.0 62 57.0 63 53.5 64 46.0 65 39.5 66 38.5 67 44.5 68 45.0 69 28.5 70 8.5 71 10.5 72 9.5 73 3.5 74 2.0 75 3.0 76 2.5 77 0.5 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.1 3 0.42500000000000004 4 0.44999999999999996 5 0.375 6 0.35000000000000003 7 0.35000000000000003 8 0.375 9 0.22499999999999998 10-14 0.505 15-19 0.79 20-24 1.325 25-29 0.715 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.33 60-64 0.0 65-69 0.08 70-74 0.43499999999999994 75-79 0.0 80-84 0.0 85-89 0.01 90-94 0.0 95-99 0.005 100-104 0.01 105-109 0.49 110-114 0.76 115-119 0.9650000000000001 120-124 0.445 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.02 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.625 #Duplication Level Percentage of deduplicated Percentage of total 1 92.6984126984127 80.30000000000001 2 4.617604617604617 8.0 3 1.1544011544011543 3.0 4 0.6349206349206349 2.1999999999999997 5 0.2597402597402597 1.125 6 0.23088023088023088 1.2 7 0.08658008658008658 0.525 8 0.02886002886002886 0.2 9 0.08658008658008658 0.675 >10 0.20202020202020202 2.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 26 0.65 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 23 0.575 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 16 0.4 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 13 0.325 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 13 0.325 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 10 0.25 No Hit AAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCG 10 0.25 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 9 0.22499999999999998 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 9 0.22499999999999998 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 9 0.22499999999999998 No Hit AACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTC 8 0.2 No Hit GTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAG 7 0.17500000000000002 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 7 0.17500000000000002 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 7 0.17500000000000002 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 6 0.15 No Hit GTAGGATAAGTGGGAGCCTTTACGGGCGCAAGTGAAATACCACTACTTTT 6 0.15 No Hit CTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCG 6 0.15 No Hit ACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCT 6 0.15 No Hit GTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 6 0.15 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 6 0.15 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 6 0.15 No Hit AAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCA 6 0.15 No Hit GATAACTCGACGGATCGCACGGCCCTCGTGCCGGCGACGCATCATTCAAA 5 0.125 No Hit GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTA 5 0.125 No Hit GACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTG 5 0.125 No Hit GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC 5 0.125 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 5 0.125 No Hit GCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAGCGTGGC 5 0.125 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 5 0.125 No Hit GTATGAACTAATTTGAACTGTGAAACTGCGAATGGCTCATTAAATCAGTT 5 0.125 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.1 0.0 0.0 0.0 0.0 96-97 0.1125 0.0 0.0 0.0 0.0 98-99 0.15 0.0 0.0 0.0 0.0 100-101 0.15 0.0 0.0 0.0 0.0 102-103 0.175 0.0 0.0 0.0 0.0 104-105 0.2 0.0 0.0 0.0 0.0 106-107 0.21250000000000002 0.0 0.0 0.0 0.0 108-109 0.225 0.0 0.0 0.0 0.0 110-111 0.2625 0.0 0.0 0.0 0.0 112-113 0.275 0.0 0.0 0.0 0.0 114-115 0.275 0.0 0.0 0.0 0.0 116-117 0.3 0.0 0.0 0.0 0.0 118-119 0.3125 0.0 0.0 0.0 0.0 120-121 0.3625 0.0 0.0 0.0 0.0 122-123 0.4375 0.0 0.0 0.0 0.0 124-125 0.5125 0.0 0.0 0.0 0.0 126-127 0.5375000000000001 0.0 0.0 0.0 0.0 128-129 0.55 0.0 0.0 0.0 0.0 130-131 0.55 0.0 0.0 0.0 0.0 132-133 0.55 0.0 0.0 0.0 0.0 134-135 0.55 0.0 0.0 0.0 0.0 136-137 0.625 0.0 0.0 0.0 0.0 138-139 0.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790480 spots for SRR6031160.sra Written 790480 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra Read 790465 spots for SRR6031160.sra Written 790465 spots for SRR6031160.sra SRR ids: ['SRR6031160.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_xx9ikbzz SRR6031160.sra spots: 15809315 blocks: [[1, 790465], [790466, 1580930], [1580931, 2371395], [2371396, 3161860], [3161861, 3952325], [3952326, 4742790], [4742791, 5533255], [5533256, 6323720], [6323721, 7114185], [7114186, 7904650], [7904651, 8695115], [8695116, 9485580], [9485581, 10276045], [10276046, 11066510], [11066511, 11856975], [11856976, 12647440], [12647441, 13437905], [13437906, 14228370], [14228371, 15018835], [15018836, 15809315]] SRR6031160 file size 5335557 SRR6031160 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6031160 SRR6031160_1.fastq SRR6031160_2.fastq Input file: SRR6031160_1.fastq Paired file: SRR6031160_2.fastq trimmed: SRR6031160-trimmed-pair1.fastq, SRR6031160-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 00:21:59 2024 >> started Tue Dec 10 00:22:18 2024 >> done (18.662s) 15809315 read pairs processed; of these: 9465 ( 0.06%) short read pairs filtered out after trimming by size control 7239 ( 0.05%) empty read pairs filtered out after trimming by size control 15792611 (99.89%) read pairs available; of these: 3557331 (22.53%) trimmed read pairs available after processing 12235280 (77.47%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 10 0.00% 20 15 0.00% 21 14 0.00% 22 18 0.00% 23 8 0.00% 24 14 0.00% 25 12 0.00% 26 15 0.00% 27 15 0.00% 28 15 0.00% 29 15 0.00% 30 11 0.00% 31 16 0.00% 32 18 0.00% 33 15 0.00% 34 13 0.00% 35 15 0.00% 36 12 0.00% 37 15 0.00% 38 18 0.00% 39 16 0.00% 40 13 0.00% 41 11 0.00% 42 18 0.00% 43 24 0.00% 44 13 0.00% 45 14 0.00% 46 24 0.00% 47 20 0.00% 48 23 0.00% 49 32 0.00% 50 25 0.00% 51 26 0.00% 52 34 0.00% 53 29 0.00% 54 44 0.00% 55 32 0.00% 56 45 0.00% 57 56 0.00% 58 62 0.00% 59 59 0.00% 60 67 0.00% 61 98 0.00% 62 75 0.00% 63 101 0.00% 64 99 0.00% 65 113 0.00% 66 134 0.00% 67 140 0.00% 68 139 0.00% 69 196 0.00% 70 203 0.00% 71 228 0.00% 72 251 0.00% 73 280 0.00% 74 291 0.00% 75 297 0.00% 76 359 0.00% 77 443 0.00% 78 406 0.00% 79 402 0.00% 80 477 0.00% 81 564 0.00% 82 644 0.00% 83 684 0.00% 84 1144 0.01% 85 1496 0.01% 86 1577 0.01% 87 1640 0.01% 88 1793 0.01% 89 1820 0.01% 90 1849 0.01% 91 1856 0.01% 92 1908 0.01% 93 2054 0.01% 94 2039 0.01% 95 2221 0.01% 96 2235 0.01% 97 2296 0.01% 98 2419 0.02% 99 2454 0.02% 100 2507 0.02% 101 2710 0.02% 102 2763 0.02% 103 3010 0.02% 104 2994 0.02% 105 3801 0.02% 106 3322 0.02% 107 3303 0.02% 108 3622 0.02% 109 3504 0.02% 110 3776 0.02% 111 4074 0.03% 112 4454 0.03% 113 4428 0.03% 114 4975 0.03% 115 5435 0.03% 116 5694 0.04% 117 5518 0.03% 118 5754 0.04% 119 5758 0.04% 120 6467 0.04% 121 6069 0.04% 122 6587 0.04% 123 7094 0.04% 124 7253 0.05% 125 7454 0.05% 126 7660 0.05% 127 7918 0.05% 128 8216 0.05% 129 8871 0.06% 130 9483 0.06% 131 10149 0.06% 132 10546 0.07% 133 11478 0.07% 134 11660 0.07% 135 12704 0.08% 136 13559 0.09% 137 14547 0.09% 138 15416 0.10% 139 16857 0.11% 140 18661 0.12% 141 20491 0.13% 142 23089 0.15% 143 26493 0.17% 144 31829 0.20% 145 40165 0.25% 146 51839 0.33% 147 74574 0.47% 148 125163 0.79% 149 307034 1.94% 150 2518198 15.95% 151 12235280 77.47% 15792611 reads passed initial QC criterion=sequence-density sequence-density=2.36 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=38 prefix-density=2.36 prefix-fanout=2.0 sequence=TACCCTTTTGTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=60.31 fanout-score-rank=1 prefix-density=0.43 prefix-fanout=1.0 sequence=CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAA criterion=sequence-density sequence-density=0.91 sequence-density-rank=1 fanout-score=5.83 fanout-score-rank=12 prefix-density=3.10 prefix-fanout=1.7 sequence=TGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCAGGAATCGCTAGTAATCGCCGGTCAGCCATACGGCGGTGAATCCGTTCCCGGGCCTTGTACACACC criterion=fanout-score sequence-density=0.03 sequence-density-rank=29 fanout-score=20.54 fanout-score-rank=1 prefix-density=0.48 prefix-fanout=1.4 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6031160 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 00:23:06 Started mapping on | Dec 10 00:23:06 Finished on | Dec 10 00:24:48 Mapping speed, Million of reads per hour | 557.39 Number of input reads | 15792611 Average input read length | 300 UNIQUE READS: Uniquely mapped reads number | 9852577 Uniquely mapped reads % | 62.39% Average mapped length | 299.70 Number of splices: Total | 9492072 Number of splices: Annotated (sjdb) | 9014398 Number of splices: GT/AG | 9363335 Number of splices: GC/AG | 111083 Number of splices: AT/AC | 6472 Number of splices: Non-canonical | 11182 Mismatch rate per base, % | 0.09% Deletion rate per base | 0.00% Deletion average length | 1.44 Insertion rate per base | 0.00% Insertion average length | 1.30 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 890989 % of reads mapped to multiple loci | 5.64% Number of reads mapped to too many loci | 416256 % of reads mapped to too many loci | 2.64% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.96% % of reads unmapped: other | 24.37% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5054570 5054570 5054570 N_multimapping 890989 890989 890989 N_noFeature 1608298 9575205 1699255 N_ambiguous 268516 2917 86471 UnstrandedReadsAssigned:7975763 PositiveStrandReadsAssigned:274455 NegativeStrandReadsAssigned:8066851 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6031160 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6031160-trimmed-pair1.fastq SRR6031160-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,792,611 reads, 8,802,544 reads pseudoaligned [quant] estimated average fragment length: 439.57 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,134 rounds 52973 SRR6031160.ke.tsv 35125 SRR6031160.se.tsv 88098 total ==> SRR6031160.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 499.227 0 0 PNS24247 1044 605.43 54.2249 13.8328 PNS24249 1928 1489.43 5.86984 0.608667 PNS24246 1044 605.43 54.2249 13.8328 PNS24248 1044 605.43 54.2249 13.8328 PNS24244 1471 1032.43 31.4556 4.70555 PNS24243 293 65.0492 0 0 KQK14069 1603 1164.43 773.725 102.624 KQK14071 474 132.837 11.3348 13.1786 ==> SRR6031160.se.tsv <== BRADI_1g14170v3 850 BRADI_1g53295v3 61 BRADI_1g59795v3 329 BRADI_1g07683v3 0 BRADI_1g00485v3 26 BRADI_1g20270v3 875 BRADI_1g74790v3 63 BRADI_1g09890v3 0 BRADI_1g77505v3 99 BRADI_1g48960v3 0 SRR6031160 completed mapping pipeline successfully