Starting /dee2/code/volunteer_pipeline.sh SRR6031168 current disk space = 1523605835776 free memory = 1599208040 SRR6031168 SRAfilesize a99a69f5da662c9c3e8a5af2f07a7cc9 SRR6031168.sra SRR6031168.sra file validated SRR6031168 is paired end SRR6031168 is conventional basespace SRR6031168 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031168_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.9305 25.0 18.0 33.0 18.0 33.0 2 24.846 25.0 18.0 31.0 18.0 33.0 3 29.683 30.0 27.0 33.0 27.0 33.0 4 31.806 33.0 31.0 33.0 29.0 33.0 5 32.70375 33.0 33.0 33.0 32.0 33.0 6 36.92375 38.0 37.0 38.0 35.0 38.0 7 37.355 38.0 38.0 38.0 36.0 38.0 8 37.5745 38.0 38.0 38.0 37.0 38.0 9 37.674 38.0 38.0 38.0 38.0 38.0 10-14 37.6893 38.0 38.0 38.0 38.0 38.0 15-19 37.69755 38.0 38.0 38.0 38.0 38.0 20-24 37.766949999999994 38.0 38.0 38.0 38.0 38.0 25-29 37.73205 38.0 38.0 38.0 38.0 38.0 30-34 37.7539 38.0 38.0 38.0 38.0 38.0 35-39 37.761 38.0 38.0 38.0 38.0 38.0 40-44 37.717099999999995 38.0 38.0 38.0 38.0 38.0 45-49 37.60485 38.0 38.0 38.0 38.0 38.0 50-54 37.64104999999999 38.0 38.0 38.0 38.0 38.0 55-59 37.602349999999994 38.0 38.0 38.0 38.0 38.0 60-64 37.541 38.0 38.0 38.0 38.0 38.0 65-69 37.5221 38.0 38.0 38.0 37.6 38.0 70-74 37.4559 38.0 38.0 38.0 37.0 38.0 75-79 37.382999999999996 38.0 38.0 38.0 37.0 38.0 80-84 37.328950000000006 38.0 38.0 38.0 37.0 38.0 85-89 37.291399999999996 38.0 38.0 38.0 36.8 38.0 90-94 37.269999999999996 38.0 38.0 38.0 36.2 38.0 95-99 37.09905 38.0 38.0 38.0 36.0 38.0 100-104 37.083549999999995 38.0 38.0 38.0 36.0 38.0 105-109 36.8973 38.0 38.0 38.0 35.0 38.0 110-114 36.871849999999995 38.0 38.0 38.0 35.0 38.0 115-119 36.638 38.0 38.0 38.0 34.6 38.0 120-124 36.4846 38.0 38.0 38.0 34.0 38.0 125-129 36.22055 38.0 37.8 38.0 33.6 38.0 130-134 35.9079 38.0 36.4 38.0 32.8 38.0 135-139 35.834649999999996 38.0 36.2 38.0 32.4 38.0 140-144 35.66725 38.0 36.0 38.0 32.4 38.0 145-149 35.25135 38.0 35.8 38.0 31.4 38.0 150-151 31.565875 36.5 31.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 0.0 16 1.0 17 0.0 18 2.0 19 1.0 20 3.0 21 1.0 22 2.0 23 2.0 24 5.0 25 3.0 26 4.0 27 14.0 28 7.0 29 16.0 30 21.0 31 23.0 32 31.0 33 59.0 34 96.0 35 224.0 36 782.0 37 2701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.45454545454545 10.328282828282829 6.666666666666667 37.55050505050505 2 23.1 9.725 32.45 34.725 3 17.95 14.274999999999999 27.425 40.35 4 24.5 22.925 21.25 31.324999999999996 5 26.0 24.349999999999998 23.925 25.724999999999998 6 24.6 29.125 24.925 21.349999999999998 7 18.5 24.925 37.2 19.375 8 19.775000000000002 21.45 29.95 28.825 9 19.425 21.05 32.025 27.500000000000004 10-14 22.475 26.525 25.724999999999998 25.275 15-19 23.03 24.525 26.815 25.629999999999995 20-24 22.935 25.61 26.195 25.259999999999998 25-29 22.470000000000002 24.375 26.484999999999996 26.669999999999998 30-34 22.225 24.610000000000003 26.21 26.955000000000002 35-39 22.68 24.505 25.545 27.27 40-44 22.84 24.38 26.279999999999998 26.5 45-49 22.765 23.830000000000002 26.090000000000003 27.315 50-54 21.560000000000002 24.32 26.205000000000002 27.915 55-59 23.265 24.27 25.645 26.82 60-64 22.12 23.474999999999998 27.05 27.355 65-69 22.07 23.49 25.919999999999998 28.52 70-74 23.189999999999998 24.63 25.435000000000002 26.745 75-79 22.89 25.585 24.154999999999998 27.37 80-84 22.115000000000002 25.19 25.540000000000003 27.155 85-89 22.95 24.445 25.86 26.745 90-94 22.435 24.91 25.740000000000002 26.915 95-99 21.035 23.945 26.46 28.560000000000002 100-104 22.46 25.105 25.41 27.025 105-109 22.495 25.61 25.900000000000002 25.995 110-114 23.365 25.86 25.05 25.724999999999998 115-119 23.585 25.255 25.025 26.135 120-124 23.5 25.28 25.485000000000003 25.735000000000003 125-129 23.47 24.925 23.735 27.87 130-134 24.474999999999998 23.225 25.314999999999998 26.985 135-139 23.925 24.595 25.15 26.33 140-144 23.21 24.79 24.515 27.485 145-149 24.27 25.014999999999997 23.945 26.77 150-151 21.7375 26.0625 24.875 27.325 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 0.5 22 1.0 23 1.0 24 0.0 25 1.0 26 2.0 27 1.5 28 2.5 29 5.0 30 7.0 31 9.0 32 13.0 33 20.0 34 25.5 35 35.0 36 46.0 37 53.5 38 65.5 39 74.0 40 72.5 41 85.5 42 103.5 43 113.0 44 126.5 45 145.5 46 177.5 47 208.0 48 211.0 49 204.0 50 210.0 51 218.0 52 208.0 53 184.0 54 173.5 55 184.0 56 167.5 57 130.5 58 126.5 59 128.0 60 107.5 61 73.5 62 49.0 63 46.0 64 40.0 65 33.0 66 21.5 67 15.5 68 20.0 69 13.5 70 11.0 71 8.0 72 5.5 73 4.5 74 2.0 75 3.0 76 3.5 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.875 #Duplication Level Percentage of deduplicated Percentage of total 1 86.54424040066779 64.8 2 6.67779632721202 10.0 3 2.671118530884808 6.0 4 1.6360601001669448 4.9 5 0.8013355592654423 3.0 6 0.5342237061769616 2.4 7 0.333889816360601 1.7500000000000002 8 0.20033388981636058 1.2 9 0.1335559265442404 0.8999999999999999 >10 0.46744574290484137 5.050000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 31 0.775 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 18 0.44999999999999996 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 17 0.42500000000000004 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 17 0.42500000000000004 No Hit ACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACG 16 0.4 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 15 0.375 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 13 0.325 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 12 0.3 No Hit GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA 11 0.27499999999999997 No Hit GTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTT 11 0.27499999999999997 No Hit CTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTC 11 0.27499999999999997 No Hit CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT 10 0.25 No Hit GTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA 10 0.25 No Hit CCCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAAT 10 0.25 No Hit CCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCT 9 0.22499999999999998 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 9 0.22499999999999998 No Hit GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC 9 0.22499999999999998 No Hit GTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGA 9 0.22499999999999998 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 8 0.2 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 8 0.2 No Hit GGCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTC 8 0.2 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 8 0.2 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 8 0.2 No Hit GTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCT 8 0.2 No Hit GGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAG 7 0.17500000000000002 No Hit GCAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG 7 0.17500000000000002 No Hit CAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC 7 0.17500000000000002 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 7 0.17500000000000002 No Hit GCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCC 7 0.17500000000000002 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 7 0.17500000000000002 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 7 0.17500000000000002 No Hit CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATT 7 0.17500000000000002 No Hit GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 7 0.17500000000000002 No Hit GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT 7 0.17500000000000002 No Hit GGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAG 6 0.15 No Hit CTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACGTCGG 6 0.15 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 6 0.15 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 6 0.15 No Hit CCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTAT 6 0.15 No Hit CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC 6 0.15 No Hit GCCCCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACA 6 0.15 No Hit GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG 6 0.15 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 6 0.15 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 6 0.15 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 6 0.15 No Hit CTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGG 6 0.15 No Hit GGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCG 6 0.15 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 6 0.15 No Hit CCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTT 6 0.15 No Hit CCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGATCTAAA 6 0.15 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 5 0.125 No Hit CTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTATTAATCATTACT 5 0.125 No Hit GTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC 5 0.125 No Hit GGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCT 5 0.125 No Hit GACCAATTGTGTGAATCAACGGTTCCTCTCGTACTAGGTTGAATTACTAT 5 0.125 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 5 0.125 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 5 0.125 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 5 0.125 No Hit CGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACT 5 0.125 No Hit CCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCC 5 0.125 No Hit CGAAGATCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTAC 5 0.125 No Hit GTGCCGTTCACATGGAACCTTTCTCCTCTTCGGCCTTCAAAGTTCTCATT 5 0.125 No Hit ATCCAGAGCGATGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGA 5 0.125 No Hit TGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTT 5 0.125 No Hit CCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC 5 0.125 No Hit CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC 5 0.125 No Hit GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTC 5 0.125 No Hit CCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGG 5 0.125 No Hit GTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCAC 5 0.125 No Hit CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCCCGA 5 0.125 No Hit CCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGAC 5 0.125 No Hit CCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGC 5 0.125 No Hit GGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGA 5 0.125 No Hit GGCACGGTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.0625 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.0875 0.0 0.0 0.0 0.0 88-89 0.1125 0.0 0.0 0.0 0.0 90-91 0.16249999999999998 0.0 0.0 0.0 0.0 92-93 0.175 0.0 0.0 0.0 0.0 94-95 0.2 0.0 0.0 0.0 0.0 96-97 0.30000000000000004 0.0 0.0 0.0 0.0 98-99 0.375 0.0 0.0 0.0 0.0 100-101 0.375 0.0 0.0 0.0 0.0 102-103 0.42500000000000004 0.0 0.0 0.0 0.0 104-105 0.5125 0.0 0.0 0.0 0.0 106-107 0.575 0.0 0.0 0.0 0.0 108-109 0.6375 0.0 0.0 0.0 0.0 110-111 0.675 0.0 0.0 0.0 0.0 112-113 0.825 0.0 0.0 0.0 0.0 114-115 0.875 0.0 0.0 0.0 0.0 116-117 0.9875 0.0 0.0 0.0 0.0 118-119 1.0 0.0 0.0 0.0 0.0 120-121 1.0375 0.0 0.0 0.0 0.0 122-123 1.1125 0.0 0.0 0.0 0.0 124-125 1.225 0.0 0.0 0.0 0.0 126-127 1.2875 0.0 0.0 0.0 0.0 128-129 1.3875 0.0 0.0 0.0 0.0 130-131 1.5375 0.0 0.0 0.0 0.0 132-133 1.5625 0.0 0.0 0.0 0.0 134-135 1.6125 0.0 0.0 0.0 0.0 136-137 1.675 0.0 0.0 0.0 0.0 138-139 1.7375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTTTA 10 0.006577216 146.82278 1 GGCAGAA 10 0.006577216 146.82278 1 TTACCTG 10 0.006832588 144.9875 5 CTTTACC 10 0.006832588 144.9875 3 AATTTGA 10 0.006832588 144.9875 7 AGAAATT 10 0.006832588 144.9875 4 GCAGAAA 10 0.006832588 144.9875 2 GCTTGGC 10 0.006832588 144.9875 145 CTCGGTC 10 0.006832588 144.9875 145 TTTACCT 10 0.006832588 144.9875 4 GCTTTAC 10 0.006832588 144.9875 2 >>END_MODULE SRR6031168 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031168_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32175 34.0 33.0 34.0 33.0 34.0 2 32.5945 34.0 33.0 34.0 33.0 34.0 3 32.6555 34.0 33.0 34.0 33.0 34.0 4 32.614 34.0 33.0 34.0 33.0 34.0 5 32.7035 34.0 33.0 34.0 33.0 34.0 6 36.802 38.0 38.0 38.0 38.0 38.0 7 36.839 38.0 38.0 38.0 38.0 38.0 8 36.853 38.0 38.0 38.0 38.0 38.0 9 36.8715 38.0 38.0 38.0 38.0 38.0 10-14 36.83475 38.0 38.0 38.0 38.0 38.0 15-19 36.71265 38.0 38.0 38.0 38.0 38.0 20-24 36.662099999999995 38.0 38.0 38.0 37.8 38.0 25-29 36.7502 38.0 38.0 38.0 37.8 38.0 30-34 37.0051 38.0 38.0 38.0 38.0 38.0 35-39 37.09955 38.0 38.0 38.0 38.0 38.0 40-44 37.13235 38.0 38.0 38.0 38.0 38.0 45-49 37.1052 38.0 38.0 38.0 38.0 38.0 50-54 37.055749999999996 38.0 38.0 38.0 38.0 38.0 55-59 36.7952 38.0 38.0 38.0 37.2 38.0 60-64 36.87945 38.0 38.0 38.0 37.0 38.0 65-69 36.79825 38.0 38.0 38.0 37.0 38.0 70-74 36.615899999999996 38.0 38.0 38.0 37.0 38.0 75-79 36.7239 38.0 38.0 38.0 37.0 38.0 80-84 36.87400000000001 38.0 38.0 38.0 37.0 38.0 85-89 36.818 38.0 38.0 38.0 36.6 38.0 90-94 36.57975 38.0 38.0 38.0 35.6 38.0 95-99 36.642999999999994 38.0 38.0 38.0 36.0 38.0 100-104 36.53565 38.0 38.0 38.0 35.6 38.0 105-109 36.26965 38.0 38.0 38.0 35.2 38.0 110-114 36.0409 38.0 38.0 38.0 34.4 38.0 115-119 35.91415000000001 38.0 38.0 38.0 34.2 38.0 120-124 35.877700000000004 38.0 38.0 38.0 34.0 38.0 125-129 36.03365 38.0 38.0 38.0 34.0 38.0 130-134 36.040350000000004 38.0 38.0 38.0 33.8 38.0 135-139 35.8719 38.0 38.0 38.0 33.0 38.0 140-144 35.505 38.0 38.0 38.0 31.6 38.0 145-149 35.1298 38.0 36.6 38.0 31.0 38.0 150-151 31.201375 35.5 30.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46.0 3 3.0 4 0.0 5 1.0 6 0.0 7 0.0 8 0.0 9 3.0 10 0.0 11 0.0 12 2.0 13 1.0 14 2.0 15 7.0 16 6.0 17 7.0 18 4.0 19 8.0 20 7.0 21 5.0 22 5.0 23 9.0 24 7.0 25 8.0 26 14.0 27 10.0 28 14.0 29 16.0 30 16.0 31 24.0 32 37.0 33 44.0 34 63.0 35 136.0 36 366.0 37 3129.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.04691930910028 22.68625934519206 8.765145656096932 26.501675689610728 2 28.188090978788654 26.55251725019167 26.986966521850242 18.272425249169437 3 22.514694607717864 24.942499361104012 29.874776386404296 22.66802964477383 4 26.42089093701997 31.36200716845878 21.32616487455197 20.89093701996928 5 28.907048008171603 30.005107252298263 20.93973442288049 20.148110316649642 6 23.813169984686063 34.30321592649311 20.26544155181215 21.61817253700868 7 21.913265306122447 24.081632653061224 33.85204081632653 20.153061224489797 8 27.35391681551416 21.587139576422558 25.338096453176828 25.72084715488645 9 27.766445690973995 21.672616012238656 28.76083630800612 21.800101988781233 10-14 26.327873871115877 27.042196030409716 24.113475177304963 22.516454921169448 15-19 26.43642875415707 25.40291634689179 25.622921463289845 22.537733435661295 20-24 27.106058278281353 25.805295232242536 24.98591693552517 22.10272955395094 25-29 27.180351307189543 26.725898692810457 24.30044934640523 21.79330065359477 30-34 26.356078272330937 26.432120044611175 25.53482713170435 21.676974551353542 35-39 26.507607541828843 27.685386442905525 23.262397007531717 22.54460900773391 40-44 27.415038125536533 25.46078876937838 24.647780639297075 22.476392465788013 45-49 26.439157173102128 25.335215243472124 25.924992438753907 22.300635144671844 50-54 25.937721160651094 26.746537256091397 25.331109089070875 21.984632494186634 55-59 25.831084864837344 26.16708242121876 25.377997250929084 22.623835463014814 60-64 28.05478062389044 24.615774790768448 25.33603854932792 21.993406036013187 65-69 28.127538586515026 24.664906580016247 24.64459788789602 22.562956945572704 70-74 27.43466721110657 25.0051041241323 24.479379338505513 23.080849326255613 75-79 26.601360267993098 23.94173180387778 25.520251751091262 23.936656177037865 80-84 27.294271883824123 25.4739814441307 24.798305768455023 22.433440903590157 85-89 27.40449947153858 24.29915949469022 25.059137349640142 23.237203684131057 90-94 27.40416099944587 25.157422799858946 24.829983376152335 22.60843282454284 95-99 27.344182999949613 25.00125963621706 24.310978989267902 23.34357837456543 100-104 26.57087928766569 25.599514317514927 26.05990083982596 21.769705554993422 105-109 26.813847957987047 24.06057206954571 27.461377657676035 21.664202314791208 110-114 27.64294108630116 25.358949466046703 25.282305451944204 21.715803995707937 115-119 27.607550258325237 25.111258887922656 24.952683001688065 22.328507852064046 120-124 28.127709491508135 24.751364308665273 23.629316060590604 23.491610139235988 125-129 27.53410813542193 25.36634663971703 23.92622536634664 23.1733198585144 130-134 27.145015105740182 25.65961732124874 23.66062437059416 23.53474320241692 135-139 26.988836367293572 24.62033591471387 24.997485668309363 23.393342049683195 140-144 27.307885484681066 25.695630336514313 24.359618282270215 22.636865896534406 145-149 26.70611242039814 25.44752544752545 24.615153186581757 23.23120894549466 150-151 27.46212121212121 26.52777777777778 23.446969696969695 22.56313131313131 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22.0 1 11.5 2 1.5 3 1.5 4 0.5 5 1.0 6 1.5 7 1.5 8 2.0 9 2.0 10 1.5 11 1.5 12 2.0 13 2.0 14 1.5 15 0.5 16 0.5 17 2.0 18 1.5 19 1.5 20 3.0 21 2.0 22 3.0 23 7.0 24 7.0 25 4.5 26 4.5 27 7.0 28 7.5 29 5.5 30 9.0 31 13.0 32 14.5 33 21.5 34 28.0 35 47.0 36 65.5 37 70.5 38 74.5 39 107.0 40 110.5 41 90.0 42 113.0 43 148.0 44 146.0 45 127.0 46 135.0 47 180.0 48 197.0 49 210.5 50 205.5 51 160.0 52 160.5 53 180.0 54 188.0 55 159.0 56 119.5 57 101.0 58 89.0 59 79.5 60 74.5 61 54.5 62 43.0 63 46.5 64 45.5 65 42.0 66 47.0 67 61.0 68 56.0 69 32.0 70 15.0 71 8.5 72 7.0 73 5.0 74 2.5 75 1.5 76 2.5 77 1.5 78 3.0 79 2.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.025 2 2.175 3 2.175 4 2.35 5 2.1 6 2.0500000000000003 7 2.0 8 2.025 9 1.95 10-14 2.005 15-19 2.275 20-24 2.365 25-29 2.08 30-34 1.37 35-39 1.085 40-44 0.985 45-49 0.8099999999999999 50-54 1.09 55-59 1.7850000000000001 60-64 1.425 65-69 1.52 70-74 2.04 75-79 1.49 80-84 0.84 85-89 0.655 90-94 0.745 95-99 0.765 100-104 1.17 105-109 1.9349999999999998 110-114 2.145 115-119 2.255 120-124 1.965 125-129 1.05 130-134 0.7000000000000001 135-139 0.5700000000000001 140-144 0.44999999999999996 145-149 0.28500000000000003 150-151 1.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.7 #Duplication Level Percentage of deduplicated Percentage of total 1 86.05834464043419 63.425 2 7.564450474898236 11.15 3 2.9850746268656714 6.6000000000000005 4 1.322930800542741 3.9 5 0.5427408412483039 2.0 6 0.44097693351424694 1.95 7 0.20352781546811397 1.05 8 0.23744911804613297 1.4000000000000001 9 0.13568521031207598 0.8999999999999999 >10 0.508819538670285 7.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 44 1.0999999999999999 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 43 1.075 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 26 0.65 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 26 0.65 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 25 0.625 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 21 0.525 No Hit GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC 20 0.5 No Hit AAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCG 17 0.42500000000000004 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 15 0.375 No Hit AACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTC 14 0.35000000000000003 No Hit GTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAG 13 0.325 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 11 0.27499999999999997 No Hit GACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTG 10 0.25 No Hit AGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAA 10 0.25 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 10 0.25 No Hit AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCA 9 0.22499999999999998 No Hit GTCCCCTCCTTTTGGCTCCAAGGCCCGGTCTGACCGGGCCGATCCGGGCG 9 0.22499999999999998 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 9 0.22499999999999998 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 9 0.22499999999999998 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 8 0.2 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 8 0.2 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 8 0.2 No Hit GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTA 8 0.2 No Hit GTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCA 8 0.2 No Hit ACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCT 8 0.2 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 8 0.2 No Hit GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG 7 0.17500000000000002 No Hit TACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGA 7 0.17500000000000002 No Hit GGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGG 7 0.17500000000000002 No Hit CTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCG 7 0.17500000000000002 No Hit GTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 7 0.17500000000000002 No Hit GAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGG 7 0.17500000000000002 No Hit GGCTCATTAAATCAGTTATAGTTTGTTTGATGGTACGTGCTACTCGGATA 6 0.15 No Hit GCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAGCGTGGC 6 0.15 No Hit GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAA 6 0.15 No Hit GTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTAC 6 0.15 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 6 0.15 No Hit GTTTAATTAAAACAAAGCATTGCGATGGTCCTCGCGGATGCTGACGCAAT 6 0.15 No Hit GTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAG 6 0.15 No Hit AGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAATCTCGTG 6 0.15 No Hit CAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 6 0.15 No Hit AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA 6 0.15 No Hit GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGGGTCGGAA 6 0.15 No Hit CAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTC 6 0.15 No Hit CGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 6 0.15 No Hit AAGTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGGGTCGG 5 0.125 No Hit CAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTA 5 0.125 No Hit CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA 5 0.125 No Hit CAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGA 5 0.125 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 5 0.125 No Hit GTGCAAGTATGAACTAATTTGAACTGTGAAACTGCGAATGGCTCATTAAA 5 0.125 No Hit GAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTA 5 0.125 No Hit GGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTC 5 0.125 No Hit CAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGG 5 0.125 No Hit GAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAG 5 0.125 No Hit GGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGC 5 0.125 No Hit GGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCC 5 0.125 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 5 0.125 No Hit CCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTC 5 0.125 No Hit ACTAATTTGAACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTT 5 0.125 No Hit TGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.0625 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.0875 0.0 0.0 0.0 0.0 88-89 0.1125 0.0 0.0 0.0 0.0 90-91 0.16249999999999998 0.0 0.0 0.0 0.0 92-93 0.175 0.0 0.0 0.0 0.0 94-95 0.2 0.0 0.0 0.0 0.0 96-97 0.30000000000000004 0.0 0.0 0.0 0.0 98-99 0.375 0.0 0.0 0.0 0.0 100-101 0.375 0.0 0.0 0.0 0.0 102-103 0.42500000000000004 0.0 0.0 0.0 0.0 104-105 0.5 0.0 0.0 0.0 0.0 106-107 0.55 0.0 0.0 0.0 0.0 108-109 0.6125 0.0 0.0 0.0 0.0 110-111 0.65 0.0 0.0 0.0 0.0 112-113 0.8 0.0 0.0 0.0 0.0 114-115 0.85 0.0 0.0 0.0 0.0 116-117 0.9624999999999999 0.0 0.0 0.0 0.0 118-119 0.975 0.0 0.0 0.0 0.0 120-121 1.0125 0.0 0.0 0.0 0.0 122-123 1.0875 0.0 0.0 0.0 0.0 124-125 1.2 0.0 0.0 0.0 0.0 126-127 1.2625 0.0 0.0 0.0 0.0 128-129 1.35 0.0 0.0 0.0 0.0 130-131 1.4625 0.0 0.0 0.0 0.0 132-133 1.4875 0.0 0.0 0.0 0.0 134-135 1.5375 0.0 0.0 0.0 0.0 136-137 1.6 0.0 0.0 0.0 0.0 138-139 1.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTCAT 10 0.00670825 145.83333 9 GCCAGTA 10 0.00670825 145.83333 4 ATAGTAA 10 0.00670825 145.83333 6 TGCCAGT 10 0.00670825 145.83333 3 >>END_MODULE Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792466 spots for SRR6031168.sra Written 792466 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra Read 792451 spots for SRR6031168.sra Written 792451 spots for SRR6031168.sra SRR ids: ['SRR6031168.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_olawqzyy SRR6031168.sra spots: 15849035 blocks: [[1, 792451], [792452, 1584902], [1584903, 2377353], [2377354, 3169804], [3169805, 3962255], [3962256, 4754706], [4754707, 5547157], [5547158, 6339608], [6339609, 7132059], [7132060, 7924510], [7924511, 8716961], [8716962, 9509412], [9509413, 10301863], [10301864, 11094314], [11094315, 11886765], [11886766, 12679216], [12679217, 13471667], [13471668, 14264118], [14264119, 15056569], [15056570, 15849035]] SRR6031168 file size 5349017 SRR6031168 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6031168 SRR6031168_1.fastq SRR6031168_2.fastq Input file: SRR6031168_1.fastq Paired file: SRR6031168_2.fastq trimmed: SRR6031168-trimmed-pair1.fastq, SRR6031168-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 00:28:53 2024 >> started Tue Dec 10 00:29:12 2024 >> done (18.339s) 15849035 read pairs processed; of these: 11139 ( 0.07%) short read pairs filtered out after trimming by size control 21245 ( 0.13%) empty read pairs filtered out after trimming by size control 15816651 (99.80%) read pairs available; of these: 4864188 (30.75%) trimmed read pairs available after processing 10952463 (69.25%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 8 0.00% 20 11 0.00% 21 7 0.00% 22 7 0.00% 23 7 0.00% 24 8 0.00% 25 8 0.00% 26 15 0.00% 27 23 0.00% 28 11 0.00% 29 17 0.00% 30 23 0.00% 31 20 0.00% 32 22 0.00% 33 23 0.00% 34 23 0.00% 35 21 0.00% 36 33 0.00% 37 23 0.00% 38 18 0.00% 39 29 0.00% 40 26 0.00% 41 37 0.00% 42 37 0.00% 43 41 0.00% 44 46 0.00% 45 46 0.00% 46 45 0.00% 47 58 0.00% 48 84 0.00% 49 63 0.00% 50 56 0.00% 51 68 0.00% 52 62 0.00% 53 93 0.00% 54 96 0.00% 55 96 0.00% 56 116 0.00% 57 115 0.00% 58 182 0.00% 59 197 0.00% 60 170 0.00% 61 191 0.00% 62 257 0.00% 63 245 0.00% 64 251 0.00% 65 302 0.00% 66 300 0.00% 67 345 0.00% 68 442 0.00% 69 540 0.00% 70 515 0.00% 71 559 0.00% 72 661 0.00% 73 785 0.00% 74 743 0.00% 75 717 0.00% 76 888 0.01% 77 977 0.01% 78 1045 0.01% 79 1225 0.01% 80 1137 0.01% 81 1356 0.01% 82 1550 0.01% 83 1633 0.01% 84 2139 0.01% 85 2664 0.02% 86 2863 0.02% 87 3319 0.02% 88 3731 0.02% 89 3150 0.02% 90 3261 0.02% 91 3499 0.02% 92 3711 0.02% 93 3926 0.02% 94 4025 0.03% 95 4398 0.03% 96 4738 0.03% 97 4925 0.03% 98 4808 0.03% 99 5301 0.03% 100 5295 0.03% 101 5540 0.04% 102 5471 0.03% 103 5563 0.04% 104 5923 0.04% 105 7544 0.05% 106 6567 0.04% 107 6440 0.04% 108 6769 0.04% 109 6766 0.04% 110 6513 0.04% 111 7477 0.05% 112 7814 0.05% 113 7762 0.05% 114 8466 0.05% 115 9086 0.06% 116 9116 0.06% 117 8925 0.06% 118 9256 0.06% 119 9617 0.06% 120 10602 0.07% 121 9647 0.06% 122 10456 0.07% 123 12432 0.08% 124 11819 0.07% 125 12174 0.08% 126 12352 0.08% 127 12328 0.08% 128 12798 0.08% 129 13604 0.09% 130 14345 0.09% 131 15120 0.10% 132 15798 0.10% 133 16391 0.10% 134 16809 0.11% 135 18068 0.11% 136 19222 0.12% 137 20672 0.13% 138 21149 0.13% 139 22680 0.14% 140 25341 0.16% 141 27259 0.17% 142 30443 0.19% 143 35858 0.23% 144 43647 0.28% 145 53576 0.34% 146 70557 0.45% 147 102189 0.65% 148 174821 1.11% 149 447918 2.83% 150 3328982 21.05% 151 10952463 69.25% 15816651 reads passed initial QC criterion=sequence-density sequence-density=3.21 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=37 prefix-density=3.21 prefix-fanout=1.9 sequence=TACCCTTTTGTT criterion=fanout-score sequence-density=0.09 sequence-density-rank=27 fanout-score=51.80 fanout-score-rank=1 prefix-density=4.43 prefix-fanout=1.0 sequence=CGGTGTGTACAGGGCCCGGGTACATATTCACCGCGGCATGCTGATCCGCGATTACTAG criterion=sequence-density sequence-density=0.90 sequence-density-rank=1 fanout-score=8.69 fanout-score-rank=10 prefix-density=5.78 prefix-fanout=1.4 sequence=TGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCAGGAATCGCTAGTAATCGCCGGTCAGCCATACGGCGGTGAATCCGTTCCCGGGCCTTGTACACACC criterion=fanout-score sequence-density=0.08 sequence-density-rank=20 fanout-score=25.53 fanout-score-rank=1 prefix-density=1.59 prefix-fanout=1.4 sequence=CCAGAGGAAACACTGGTGGAGGC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x TACCCTTTTGTT -y TGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCAGGAATCGCTAGTAATCGCCGGTCAGCCATACGGCGGTGAATCCGTTCCCGGGCCTTGTACACACC -o SRR6031168 SRR6031168_1.fastq SRR6031168_2.fastq Input file: SRR6031168_1.fastq Paired file: SRR6031168_2.fastq trimmed: SRR6031168-trimmed-pair1.fastq, SRR6031168-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): TACCCTTTTGTT -- paired 3' end adapter sequence (-y): TGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 00:30:38 2024 >> started Tue Dec 10 00:30:44 2024 >> done (5.982s) 5272217 read pairs processed; of these: 230 ( 0.00%) short read pairs filtered out after trimming by size control 375 ( 0.01%) empty read pairs filtered out after trimming by size control 5271612 (99.99%) read pairs available; of these: 137 ( 0.00%) trimmed read pairs available after processing 5271475 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 3 0.00% 20 5 0.00% 21 6 0.00% 22 4 0.00% 23 5 0.00% 24 3 0.00% 25 7 0.00% 26 8 0.00% 27 13 0.00% 28 5 0.00% 29 2 0.00% 30 6 0.00% 31 2 0.00% 32 5 0.00% 33 4 0.00% 34 5 0.00% 35 7 0.00% 36 12 0.00% 37 8 0.00% 38 7 0.00% 39 11 0.00% 40 7 0.00% 41 9 0.00% 42 6 0.00% 43 16 0.00% 44 22 0.00% 45 14 0.00% 46 10 0.00% 47 17 0.00% 48 28 0.00% 49 23 0.00% 50 14 0.00% 51 24 0.00% 52 19 0.00% 53 32 0.00% 54 37 0.00% 55 28 0.00% 56 43 0.00% 57 32 0.00% 58 61 0.00% 59 48 0.00% 60 59 0.00% 61 58 0.00% 62 83 0.00% 63 82 0.00% 64 99 0.00% 65 96 0.00% 66 98 0.00% 67 126 0.00% 68 149 0.00% 69 182 0.00% 70 174 0.00% 71 187 0.00% 72 224 0.00% 73 277 0.01% 74 268 0.01% 75 244 0.00% 76 317 0.01% 77 310 0.01% 78 349 0.01% 79 404 0.01% 80 376 0.01% 81 450 0.01% 82 506 0.01% 83 555 0.01% 84 729 0.01% 85 913 0.02% 86 956 0.02% 87 1119 0.02% 88 1229 0.02% 89 1023 0.02% 90 1112 0.02% 91 1190 0.02% 92 1212 0.02% 93 1305 0.02% 94 1325 0.03% 95 1467 0.03% 96 1601 0.03% 97 1756 0.03% 98 1588 0.03% 99 1721 0.03% 100 1810 0.03% 101 1884 0.04% 102 1836 0.03% 103 1831 0.03% 104 1999 0.04% 105 2467 0.05% 106 2182 0.04% 107 2219 0.04% 108 2275 0.04% 109 2244 0.04% 110 2177 0.04% 111 2445 0.05% 112 2630 0.05% 113 2568 0.05% 114 2836 0.05% 115 3064 0.06% 116 3069 0.06% 117 2956 0.06% 118 3074 0.06% 119 3248 0.06% 120 3597 0.07% 121 3245 0.06% 122 3452 0.07% 123 4246 0.08% 124 3961 0.08% 125 4083 0.08% 126 3985 0.08% 127 4091 0.08% 128 4243 0.08% 129 4515 0.09% 130 4746 0.09% 131 5168 0.10% 132 5351 0.10% 133 5544 0.11% 134 5629 0.11% 135 5940 0.11% 136 6352 0.12% 137 6988 0.13% 138 6963 0.13% 139 7510 0.14% 140 8388 0.16% 141 9073 0.17% 142 10220 0.19% 143 11987 0.23% 144 14497 0.28% 145 17803 0.34% 146 23751 0.45% 147 33972 0.64% 148 58569 1.11% 149 149048 2.83% 150 1109758 21.05% 151 3649581 69.23% criterion=sequence-density sequence-density=1.90 sequence-density-rank=1 fanout-score=1.70 fanout-score-rank=38 prefix-density=3.23 prefix-fanout=1.0 sequence=CGACGGTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGATCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTTGCCCCGCTTCCGACCCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTAT criterion=fanout-score sequence-density=0.08 sequence-density-rank=26 fanout-score=52.39 fanout-score-rank=1 prefix-density=4.40 prefix-fanout=1.0 sequence=CGGTGTGTACAGGGCCCGGGTACATATTCACCGCGGCATGCTGATCCGCGATTACTAG criterion=sequence-density sequence-density=0.92 sequence-density-rank=1 fanout-score=8.55 fanout-score-rank=10 prefix-density=5.78 prefix-fanout=1.4 sequence=TGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCAGGAATCGCTAGTAATCGCCGGTCAGCCATACGGCGGTGAATCCGTTCCCGGGCCTTGTACACACC criterion=fanout-score sequence-density=0.09 sequence-density-rank=20 fanout-score=25.58 fanout-score-rank=1 prefix-density=1.61 prefix-fanout=1.4 sequence=CCAGAGGAAACACTGGTGGAGGC SRR6031168 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 00:31:40 Started mapping on | Dec 10 00:31:41 Finished on | Dec 10 00:33:56 Mapping speed, Million of reads per hour | 421.76 Number of input reads | 15816046 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 6084947 Uniquely mapped reads % | 38.47% Average mapped length | 298.96 Number of splices: Total | 4892334 Number of splices: Annotated (sjdb) | 4644169 Number of splices: GT/AG | 4824670 Number of splices: GC/AG | 55667 Number of splices: AT/AC | 3397 Number of splices: Non-canonical | 8600 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.01% Deletion average length | 1.49 Insertion rate per base | 0.00% Insertion average length | 1.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 950702 % of reads mapped to multiple loci | 6.01% Number of reads mapped to too many loci | 929035 % of reads mapped to too many loci | 5.87% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.28% % of reads unmapped: other | 45.36% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 8783862 8783862 8783862 N_multimapping 950702 950702 950702 N_noFeature 1436209 5907208 1496789 N_ambiguous 186780 2278 73511 UnstrandedReadsAssigned:4461958 PositiveStrandReadsAssigned:175461 NegativeStrandReadsAssigned:4514647 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6031168 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6031168-trimmed-pair1.fastq SRR6031168-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,816,046 reads, 5,160,065 reads pseudoaligned [quant] estimated average fragment length: 424.798 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 987 rounds 52973 SRR6031168.ke.tsv 35125 SRR6031168.se.tsv 88098 total ==> SRR6031168.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 513.549 0 0 PNS24247 1044 620.202 26.8387 10.5715 PNS24249 1928 1504.2 9.2847 1.5079 PNS24246 1044 620.202 26.8387 10.5715 PNS24248 1044 620.202 26.8387 10.5715 PNS24244 1471 1047.2 12.1992 2.84584 PNS24243 293 71.8269 0 0 KQK14069 1603 1179.2 755.088 156.43 KQK14071 474 141.456 9.3026 16.0654 ==> SRR6031168.se.tsv <== BRADI_1g14170v3 922 BRADI_1g53295v3 35 BRADI_1g59795v3 163 BRADI_1g07683v3 0 BRADI_1g00485v3 8 BRADI_1g20270v3 320 BRADI_1g74790v3 44 BRADI_1g09890v3 0 BRADI_1g77505v3 57 BRADI_1g48960v3 0 SRR6031168 completed mapping pipeline successfully