Starting /dee2/code/volunteer_pipeline.sh SRR6031183 current disk space = 1523644428288 free memory = 1400788972 SRR6031183 SRAfilesize 73f7a860af44690a214c113d44570687 SRR6031183.sra SRR6031183.sra file validated SRR6031183 is paired end SRR6031183 is conventional basespace SRR6031183 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031183_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5885 32.0 32.0 33.0 30.0 33.0 2 32.96275 33.0 33.0 33.0 33.0 34.0 3 33.162 33.0 33.0 34.0 33.0 34.0 4 33.49 34.0 33.0 34.0 33.0 34.0 5 33.6815 34.0 34.0 34.0 33.0 34.0 6 37.62525 38.0 38.0 38.0 37.0 38.0 7 37.8285 38.0 38.0 38.0 38.0 38.0 8 37.85575 38.0 38.0 38.0 38.0 38.0 9 37.89025 38.0 38.0 38.0 38.0 38.0 10-14 37.88955 38.0 38.0 38.0 38.0 38.0 15-19 37.89085 38.0 38.0 38.0 38.0 38.0 20-24 37.878049999999995 38.0 38.0 38.0 38.0 38.0 25-29 37.8408 38.0 38.0 38.0 38.0 38.0 30-34 37.86215 38.0 38.0 38.0 38.0 38.0 35-39 37.859449999999995 38.0 38.0 38.0 38.0 38.0 40-44 37.82285 38.0 38.0 38.0 38.0 38.0 45-49 37.808550000000004 38.0 38.0 38.0 38.0 38.0 50-54 37.7847 38.0 38.0 38.0 38.0 38.0 55-59 37.77875 38.0 38.0 38.0 38.0 38.0 60-64 37.77105 38.0 38.0 38.0 38.0 38.0 65-69 37.7515 38.0 38.0 38.0 38.0 38.0 70-74 37.7158 38.0 38.0 38.0 38.0 38.0 75-79 37.675399999999996 38.0 38.0 38.0 38.0 38.0 80-84 37.6927 38.0 38.0 38.0 38.0 38.0 85-89 37.6177 38.0 38.0 38.0 38.0 38.0 90-94 37.594550000000005 38.0 38.0 38.0 38.0 38.0 95-99 37.54015 38.0 38.0 38.0 38.0 38.0 100-104 37.495149999999995 38.0 38.0 38.0 38.0 38.0 105-109 37.45485 38.0 38.0 38.0 37.8 38.0 110-114 37.4174 38.0 38.0 38.0 37.8 38.0 115-119 37.320800000000006 38.0 38.0 38.0 36.8 38.0 120-124 37.24615 38.0 38.0 38.0 36.0 38.0 125-129 37.19435 38.0 38.0 38.0 36.0 38.0 130-134 37.056599999999996 38.0 38.0 38.0 35.8 38.0 135-139 37.0017 38.0 38.0 38.0 35.4 38.0 140-144 36.8549 38.0 38.0 38.0 35.0 38.0 145-149 36.774699999999996 38.0 38.0 38.0 35.0 38.0 150-151 34.912625000000006 38.0 36.0 38.0 29.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.0 17 0.0 18 1.0 19 2.0 20 0.0 21 1.0 22 2.0 23 0.0 24 1.0 25 3.0 26 4.0 27 6.0 28 2.0 29 7.0 30 5.0 31 8.0 32 13.0 33 23.0 34 31.0 35 65.0 36 240.0 37 3582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.098171800651144 10.217881292261458 4.3826696719258695 29.301277235161532 2 20.625 9.6 36.35 33.425 3 18.224999999999998 13.875000000000002 26.5 41.4 4 25.85 20.3 21.6 32.25 5 26.950000000000003 23.724999999999998 23.425 25.900000000000002 6 24.580305687797544 28.990228013029316 23.352543222250063 23.076923076923077 7 18.125 24.7 36.05 21.125 8 19.3 20.625 32.824999999999996 27.250000000000004 9 19.225 20.825 32.775 27.175 10-14 23.355 25.94 25.28 25.424999999999997 15-19 23.16 23.025000000000002 27.195000000000004 26.619999999999997 20-24 22.915 24.89 26.31 25.885 25-29 22.634999999999998 24.310000000000002 25.374999999999996 27.68 30-34 23.165 22.97 25.905 27.96 35-39 22.425 23.94 25.624999999999996 28.01 40-44 23.419999999999998 23.565 26.0 27.015 45-49 23.225 23.275000000000002 25.91 27.589999999999996 50-54 22.2 23.945 25.814999999999998 28.04 55-59 23.61 23.18 25.405 27.805000000000003 60-64 22.41 22.655 27.229999999999997 27.705000000000002 65-69 22.455 23.54 26.58 27.425 70-74 23.25 24.255 25.080000000000002 27.415 75-79 23.215 24.95 23.380000000000003 28.455000000000002 80-84 22.259999999999998 24.349999999999998 25.47 27.92 85-89 23.345 23.225 25.91 27.52 90-94 22.475 24.89 25.590000000000003 27.045 95-99 21.5 23.119999999999997 26.169999999999998 29.21 100-104 23.98 23.494999999999997 25.16 27.365000000000002 105-109 23.095 24.295 26.055 26.555 110-114 23.91 24.59 25.4 26.1 115-119 24.68 23.544999999999998 24.34 27.435 120-124 24.245 25.074999999999996 24.725 25.955000000000002 125-129 24.03 24.715 22.705000000000002 28.549999999999997 130-134 24.605 23.285 25.215 26.895000000000003 135-139 23.805 23.93 24.25 28.015 140-144 24.07 24.245 23.549999999999997 28.134999999999998 145-149 24.05 23.685000000000002 24.525 27.74 150-151 22.340292536567073 25.928241030128767 24.94061757719715 26.790848856107015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 1.5 26 1.5 27 1.0 28 1.0 29 2.0 30 3.0 31 5.0 32 6.5 33 8.0 34 13.5 35 21.0 36 29.0 37 34.0 38 42.0 39 53.5 40 61.5 41 80.5 42 98.5 43 108.5 44 125.0 45 154.5 46 189.0 47 219.0 48 229.5 49 246.0 50 251.0 51 233.0 52 218.5 53 176.5 54 140.5 55 157.0 56 155.5 57 125.5 58 121.0 59 126.5 60 106.5 61 80.0 62 64.5 63 48.0 64 47.0 65 49.5 66 34.0 67 21.0 68 25.0 69 19.5 70 12.5 71 10.5 72 11.5 73 10.5 74 5.0 75 5.0 76 6.5 77 2.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.22499999999999998 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.55 #Duplication Level Percentage of deduplicated Percentage of total 1 84.6917080085046 59.75 2 7.547838412473423 10.65 3 3.0120481927710845 6.375 4 1.2756909992912826 3.5999999999999996 5 0.9922041105598866 3.5000000000000004 6 0.5669737774627923 2.4 7 0.5315379163713678 2.625 8 0.4606661941885188 2.6 9 0.1771793054571226 1.125 >10 0.744153082919915 7.375 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 22 0.5499999999999999 No Hit GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 21 0.525 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 20 0.5 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 19 0.475 No Hit GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTA 18 0.44999999999999996 No Hit GTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA 17 0.42500000000000004 No Hit CCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCT 16 0.4 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 16 0.4 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 15 0.375 No Hit GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT 15 0.375 No Hit GTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAG 12 0.3 No Hit GTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCT 12 0.3 No Hit GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA 11 0.27499999999999997 No Hit GTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCG 11 0.27499999999999997 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 10 0.25 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 10 0.25 No Hit CCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTAT 10 0.25 No Hit CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC 10 0.25 No Hit GCCCACTTGGAGCTCCCGATTCCATGGCGCGGCTCACCGGAGCAGCCGCG 10 0.25 No Hit GCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCATAA 10 0.25 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 10 0.25 No Hit CCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGC 9 0.22499999999999998 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 9 0.22499999999999998 No Hit GTCAAATTAAGCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTC 9 0.22499999999999998 No Hit GCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCC 9 0.22499999999999998 No Hit GTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTT 9 0.22499999999999998 No Hit CTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTT 8 0.2 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 8 0.2 No Hit CCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTA 8 0.2 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 8 0.2 No Hit GCAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG 8 0.2 No Hit CTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTC 8 0.2 No Hit GGCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTC 8 0.2 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 8 0.2 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 8 0.2 No Hit GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC 8 0.2 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 8 0.2 No Hit GTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCAC 8 0.2 No Hit CCCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAAT 8 0.2 No Hit CTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTATTAATCATTACT 7 0.17500000000000002 No Hit GCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCC 7 0.17500000000000002 No Hit ATCGCGGCACGGTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC 7 0.17500000000000002 No Hit ACTCATTCCAATTACCAGACACTAATGTGCCCGGTATTGTTATTTATTGT 7 0.17500000000000002 No Hit GTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC 7 0.17500000000000002 No Hit CCCCCATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTT 7 0.17500000000000002 No Hit GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTT 7 0.17500000000000002 No Hit GACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACGTC 7 0.17500000000000002 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 7 0.17500000000000002 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 7 0.17500000000000002 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 7 0.17500000000000002 No Hit GTTCAGTCATAATCCGGCACACGGTAGCTTCGCGCCACTGGCTTTTCAAC 7 0.17500000000000002 No Hit CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCCCGA 7 0.17500000000000002 No Hit GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC 7 0.17500000000000002 No Hit CCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGATCTAAA 7 0.17500000000000002 No Hit GGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAG 6 0.15 No Hit GTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTT 6 0.15 No Hit GCCCTCCAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATT 6 0.15 No Hit CCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG 6 0.15 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 6 0.15 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 6 0.15 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 6 0.15 No Hit ACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTC 6 0.15 No Hit GCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGC 6 0.15 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 6 0.15 No Hit GTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGC 6 0.15 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 6 0.15 No Hit GGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCAC 6 0.15 No Hit GTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGA 6 0.15 No Hit GGCACGGTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC 6 0.15 No Hit GGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCC 6 0.15 No Hit ACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACG 5 0.125 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA 5 0.125 No Hit GTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAG 5 0.125 No Hit GGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTT 5 0.125 No Hit GTCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG 5 0.125 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 5 0.125 No Hit CCCATGCTAATGTATCCAGAGCGATGGCTTGCTTTGAGCACTCTAATTTC 5 0.125 No Hit GACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGG 5 0.125 No Hit GGCCCACTTGGAGCTCCCGATTCCATGGCGCGGCTCACCGGAGCAGCCGC 5 0.125 No Hit GTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCC 5 0.125 No Hit GCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCACGACCC 5 0.125 No Hit GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG 5 0.125 No Hit GCCCCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACA 5 0.125 No Hit CATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAAT 5 0.125 No Hit GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC 5 0.125 No Hit ATCCCATGCTAATGTATCCAGAGCGATGGCTTGCTTTGAGCACTCTAATT 5 0.125 No Hit CTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGT 5 0.125 No Hit CGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACT 5 0.125 No Hit GGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCG 5 0.125 No Hit GGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCC 5 0.125 No Hit GGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTA 5 0.125 No Hit GGCTTTACCTGATAGAACTCGTAATGGGCTCCAGCTATCCTGAGGGAAAC 5 0.125 No Hit CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC 5 0.125 No Hit CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATT 5 0.125 No Hit CCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTT 5 0.125 No Hit CCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGAC 5 0.125 No Hit GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA 5 0.125 No Hit CCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.21250000000000002 0.0 0.0 0.0 0.0 86-87 0.2375 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.3625 0.0 0.0 0.0 0.0 94-95 0.3875 0.0 0.0 0.0 0.0 96-97 0.4375 0.0 0.0 0.0 0.0 98-99 0.45 0.0 0.0 0.0 0.0 100-101 0.475 0.0 0.0 0.0 0.0 102-103 0.475 0.0 0.0 0.0 0.0 104-105 0.4875 0.0 0.0 0.0 0.0 106-107 0.525 0.0 0.0 0.0 0.0 108-109 0.5375000000000001 0.0 0.0 0.0 0.0 110-111 0.625 0.0 0.0 0.0 0.0 112-113 0.7 0.0 0.0 0.0 0.0 114-115 0.775 0.0 0.0 0.0 0.0 116-117 0.8374999999999999 0.0 0.0 0.0 0.0 118-119 0.875 0.0 0.0 0.0 0.0 120-121 0.9 0.0 0.0 0.0 0.0 122-123 0.9375 0.0 0.0 0.0 0.0 124-125 1.0125 0.0 0.0 0.0 0.0 126-127 1.0750000000000002 0.0 0.0 0.0 0.0 128-129 1.1749999999999998 0.0 0.0 0.0 0.0 130-131 1.2875 0.0 0.0 0.0 0.0 132-133 1.375 0.0 0.0 0.0 0.0 134-135 1.4249999999999998 0.0 0.0 0.0 0.0 136-137 1.4625 0.0 0.0 0.0 0.0 138-139 1.5625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6031183 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031183_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.28925 34.0 33.0 34.0 33.0 34.0 2 33.50925 34.0 33.0 34.0 33.0 34.0 3 33.55975 34.0 33.0 34.0 33.0 34.0 4 33.48375 34.0 33.0 34.0 33.0 34.0 5 33.525 34.0 33.0 34.0 33.0 34.0 6 37.68625 38.0 38.0 38.0 38.0 38.0 7 37.17125 38.0 38.0 38.0 37.0 38.0 8 37.55125 38.0 38.0 38.0 38.0 38.0 9 37.58425 38.0 38.0 38.0 38.0 38.0 10-14 37.647000000000006 38.0 38.0 38.0 38.0 38.0 15-19 37.60045 38.0 38.0 38.0 38.0 38.0 20-24 37.59215 38.0 38.0 38.0 38.0 38.0 25-29 37.6414 38.0 38.0 38.0 38.0 38.0 30-34 37.75605 38.0 38.0 38.0 38.0 38.0 35-39 37.738350000000004 38.0 38.0 38.0 38.0 38.0 40-44 37.715050000000005 38.0 38.0 38.0 38.0 38.0 45-49 37.733250000000005 38.0 38.0 38.0 38.0 38.0 50-54 37.7308 38.0 38.0 38.0 38.0 38.0 55-59 37.6921 38.0 38.0 38.0 38.0 38.0 60-64 37.70855 38.0 38.0 38.0 38.0 38.0 65-69 37.70985 38.0 38.0 38.0 38.0 38.0 70-74 37.54775 38.0 38.0 38.0 38.0 38.0 75-79 37.64665000000001 38.0 38.0 38.0 38.0 38.0 80-84 37.626050000000006 38.0 38.0 38.0 38.0 38.0 85-89 37.61945 38.0 38.0 38.0 38.0 38.0 90-94 37.587450000000004 38.0 38.0 38.0 38.0 38.0 95-99 37.5569 38.0 38.0 38.0 38.0 38.0 100-104 37.49905 38.0 38.0 38.0 38.0 38.0 105-109 37.34455 38.0 38.0 38.0 38.0 38.0 110-114 37.326699999999995 38.0 38.0 38.0 38.0 38.0 115-119 37.286500000000004 38.0 38.0 38.0 38.0 38.0 120-124 37.2841 38.0 38.0 38.0 37.8 38.0 125-129 37.28115 38.0 38.0 38.0 37.4 38.0 130-134 37.210300000000004 38.0 38.0 38.0 36.4 38.0 135-139 37.201499999999996 38.0 38.0 38.0 36.0 38.0 140-144 37.13595 38.0 38.0 38.0 36.0 38.0 145-149 36.97285000000001 38.0 38.0 38.0 36.0 38.0 150-151 34.957875 38.0 36.0 38.0 30.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 0.0 15 1.0 16 0.0 17 0.0 18 2.0 19 1.0 20 1.0 21 3.0 22 1.0 23 1.0 24 3.0 25 4.0 26 6.0 27 8.0 28 7.0 29 7.0 30 11.0 31 11.0 32 15.0 33 24.0 34 37.0 35 58.0 36 186.0 37 3607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.325 20.375 5.55 24.75 2 28.875 24.4 27.075 19.650000000000002 3 24.48112028007002 25.10627656914228 27.25681420355089 23.15578894723681 4 28.953953953953953 29.754754754754753 20.295295295295297 20.995995995995994 5 30.21510755377689 30.265132566283143 20.535267633816908 18.98449224612306 6 25.25 33.95 19.650000000000002 21.15 7 22.975 23.025000000000002 31.900000000000002 22.1 8 27.122464312546956 22.364137240170297 24.3175557225144 26.195842724768344 9 26.710097719869708 21.92432974191932 27.38661989476322 23.978952643447755 10-14 26.450708456416162 27.111600660892204 23.08115956541331 23.356531317278325 15-19 27.583095202286056 24.775655487040655 24.28936682207851 23.35188248859478 20-24 27.89460476787955 25.69134253450439 24.416562107904642 21.99749058971142 25-29 27.563942139246205 26.50783322488613 23.87006356674508 22.05816106912258 30-34 26.915 26.145000000000003 24.7 22.24 35-39 26.88 27.435 21.815 23.87 40-44 27.755000000000003 24.97 24.29 22.985 45-49 27.295 25.045 24.325 23.335 50-54 26.795 26.400000000000002 23.97 22.835 55-59 26.411602900725185 25.661415353838457 24.571142785696424 23.355838959739934 60-64 28.299999999999997 24.19 24.169999999999998 23.34 65-69 28.285 24.759999999999998 23.69 23.265 70-74 27.840424998747054 24.778228837768758 23.269683756828545 24.11166240665564 75-79 27.725 23.575 24.675 24.025 80-84 28.694999999999997 24.474999999999998 23.425 23.405 85-89 28.389999999999997 23.45 23.825 24.335 90-94 28.810000000000002 24.055 23.715 23.419999999999998 95-99 27.775 24.065 24.09 24.07 100-104 27.66 24.46 25.365 22.515 105-109 27.645701675193095 23.477781121476575 26.311565854147855 22.564951349182465 110-114 28.395681646999748 24.82550841074567 24.263118252573438 22.515691689681145 115-119 28.52409638554217 24.533132530120483 23.40863453815261 23.53413654618474 120-124 28.720953525641026 24.283854166666664 23.232171474358974 23.763020833333336 125-129 27.565 25.52 23.175 23.74 130-134 27.87 25.169999999999998 22.875 24.085 135-139 27.675 24.215 24.349999999999998 23.76 140-144 28.26 25.31 23.315 23.115 145-149 26.640000000000004 25.624999999999996 23.555 24.18 150-151 28.7 25.575 22.900000000000002 22.825 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 1.0 26 1.0 27 1.0 28 2.0 29 2.5 30 4.5 31 5.5 32 7.0 33 10.5 34 13.0 35 26.0 36 39.0 37 43.5 38 51.5 39 90.5 40 101.5 41 82.0 42 113.0 43 144.0 44 148.5 45 146.0 46 156.5 47 192.5 48 207.5 49 222.0 50 209.5 51 177.0 52 176.0 53 201.5 54 189.5 55 135.0 56 104.5 57 85.0 58 88.0 59 85.0 60 69.0 61 53.0 62 46.5 63 48.5 64 54.0 65 61.5 66 61.0 67 82.0 68 94.5 69 58.5 70 27.0 71 22.0 72 16.0 73 7.5 74 7.0 75 7.0 76 7.0 77 6.0 78 4.5 79 3.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.025 4 0.1 5 0.05 6 0.0 7 0.0 8 0.17500000000000002 9 0.22499999999999998 10-14 0.135 15-19 0.265 20-24 0.375 25-29 0.105 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.025 60-64 0.0 65-69 0.0 70-74 0.23500000000000001 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.31 110-114 0.42500000000000004 115-119 0.4 120-124 0.16 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.85 #Duplication Level Percentage of deduplicated Percentage of total 1 82.57184966838615 56.025000000000006 2 9.174649963154016 12.45 3 3.831982313927782 7.8 4 1.5106853352984526 4.1000000000000005 5 0.6632277081798084 2.25 6 0.552689756816507 2.25 7 0.3316138540899042 1.575 8 0.2579218865143699 1.4000000000000001 9 0.14738393515106854 0.8999999999999999 >10 0.9211495946941783 9.775 >50 0.036845983787767135 1.4749999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 59 1.4749999999999999 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 42 1.05 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 32 0.8 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 25 0.625 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 22 0.5499999999999999 No Hit GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC 20 0.5 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 19 0.475 No Hit GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAA 18 0.44999999999999996 No Hit AAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCG 16 0.4 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 15 0.375 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 15 0.375 No Hit AACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTC 14 0.35000000000000003 No Hit ACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCT 14 0.35000000000000003 No Hit GGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAG 12 0.3 No Hit GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGGGTCGGAA 12 0.3 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 11 0.27499999999999997 No Hit GTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCA 11 0.27499999999999997 No Hit GGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTT 11 0.27499999999999997 No Hit GTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAG 11 0.27499999999999997 No Hit CAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 11 0.27499999999999997 No Hit GCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAGCGTGGC 10 0.25 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 10 0.25 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 10 0.25 No Hit GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTA 10 0.25 No Hit GTTTAATTAAAACAAAGCATTGCGATGGTCCTCGCGGATGCTGACGCAAT 10 0.25 No Hit GTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 10 0.25 No Hit CTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCG 9 0.22499999999999998 No Hit GCCAGAGGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCG 9 0.22499999999999998 No Hit GAAGAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAGCAG 9 0.22499999999999998 No Hit GGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACT 9 0.22499999999999998 No Hit CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA 8 0.2 No Hit GCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTG 8 0.2 No Hit GACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTG 8 0.2 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 8 0.2 No Hit GTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGGAGGG 8 0.2 No Hit GATTAAGCCATGCATGTGCAAGTATGAACTAATTTGAACTGTGAAACTGC 8 0.2 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 8 0.2 No Hit AGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGT 7 0.17500000000000002 No Hit GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG 7 0.17500000000000002 No Hit CGTTATTTTACTTATTCCGTGGGTCGGAAGCGGGGCAAGTCCCCTCCTTT 7 0.17500000000000002 No Hit CGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGT 7 0.17500000000000002 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 7 0.17500000000000002 No Hit GGTAAAGCCAATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATT 7 0.17500000000000002 No Hit CCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATT 7 0.17500000000000002 No Hit CATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGG 7 0.17500000000000002 No Hit GCTCATTAAATCAGTTATAGTTTGTTTGATGGTACGTGCTACTCGGATAA 7 0.17500000000000002 No Hit GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA 6 0.15 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 6 0.15 No Hit GTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACC 6 0.15 No Hit GTATGAACTAATTTGAACTGTGAAACTGCGAATGGCTCATTAAATCAGTT 6 0.15 No Hit GTAGGATAAGTGGGAGCCTTTACGGGCGCAAGTGAAATACCACTACTTTT 6 0.15 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 6 0.15 No Hit GGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGG 6 0.15 No Hit GCAAGTATGAACTAATTTGAACTGTGAAACTGCGAATGGCTCATTAAATC 6 0.15 No Hit CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA 6 0.15 No Hit CGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGATTC 6 0.15 No Hit GTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTAC 6 0.15 No Hit GAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTA 6 0.15 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 6 0.15 No Hit GATTAATAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA 6 0.15 No Hit GGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTAC 6 0.15 No Hit GTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAATAGGGACAGT 5 0.125 No Hit GGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAG 5 0.125 No Hit CACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTG 5 0.125 No Hit AAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTT 5 0.125 No Hit GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTA 5 0.125 No Hit GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTA 5 0.125 No Hit GGATTAACGAGATTCCCACTGTCCCTGTCTACTATCCAGCGAAACCACAG 5 0.125 No Hit CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTTGAAC 5 0.125 No Hit CAGAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGATTCTGATTT 5 0.125 No Hit AAGCCATGCATGTGCAAGTATGAACTAATTTGAACTGTGAAACTGCGAAT 5 0.125 No Hit GAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGATTCTGATTTCC 5 0.125 No Hit GCGTGGCCTATCGATCCTTTAGATCTTCGGAGTTTGAAGCTAGAGGTGTC 5 0.125 No Hit GAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCC 5 0.125 No Hit GTAATGATTAATAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGG 5 0.125 No Hit CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATG 5 0.125 No Hit GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTAT 5 0.125 No Hit CGATGATTCATGATAACTCGACGGATCGCACGGCCCTCGTGCCGGCGACG 5 0.125 No Hit AATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.21250000000000002 0.0 0.0 0.0 0.0 86-87 0.2375 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.3625 0.0 0.0 0.0 0.0 94-95 0.3875 0.0 0.0 0.0 0.0 96-97 0.4375 0.0 0.0 0.0 0.0 98-99 0.45 0.0 0.0 0.0 0.0 100-101 0.475 0.0 0.0 0.0 0.0 102-103 0.475 0.0 0.0 0.0 0.0 104-105 0.4875 0.0 0.0 0.0 0.0 106-107 0.525 0.0 0.0 0.0 0.0 108-109 0.5375000000000001 0.0 0.0 0.0 0.0 110-111 0.6125 0.0 0.0 0.0 0.0 112-113 0.675 0.0 0.0 0.0 0.0 114-115 0.75 0.0 0.0 0.0 0.0 116-117 0.8125 0.0 0.0 0.0 0.0 118-119 0.85 0.0 0.0 0.0 0.0 120-121 0.875 0.0 0.0 0.0 0.0 122-123 0.9125000000000001 0.0 0.0 0.0 0.0 124-125 0.9875 0.0 0.0 0.0 0.0 126-127 1.0499999999999998 0.0 0.0 0.0 0.0 128-129 1.1375 0.0 0.0 0.0 0.0 130-131 1.2374999999999998 0.0 0.0 0.0 0.0 132-133 1.325 0.0 0.0 0.0 0.0 134-135 1.375 0.0 0.0 0.0 0.0 136-137 1.4125 0.0 0.0 0.0 0.0 138-139 1.5125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAAAAG 25 8.7132835E-4 87.0 3 >>END_MODULE Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra Read 330154 spots for SRR6031183.sra Written 330154 spots for SRR6031183.sra SRR ids: ['SRR6031183.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_fm9io7k2 SRR6031183.sra spots: 6603080 blocks: [[1, 330154], [330155, 660308], [660309, 990462], [990463, 1320616], [1320617, 1650770], [1650771, 1980924], [1980925, 2311078], [2311079, 2641232], [2641233, 2971386], [2971387, 3301540], [3301541, 3631694], [3631695, 3961848], [3961849, 4292002], [4292003, 4622156], [4622157, 4952310], [4952311, 5282464], [5282465, 5612618], [5612619, 5942772], [5942773, 6272926], [6272927, 6603080]] SRR6031183 file size 2222501 SRR6031183 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6031183 SRR6031183_1.fastq SRR6031183_2.fastq Input file: SRR6031183_1.fastq Paired file: SRR6031183_2.fastq trimmed: SRR6031183-trimmed-pair1.fastq, SRR6031183-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 00:40:27 2024 >> started Tue Dec 10 00:40:35 2024 >> done (8.047s) 6603080 read pairs processed; of these: 2652 ( 0.04%) short read pairs filtered out after trimming by size control 2512 ( 0.04%) empty read pairs filtered out after trimming by size control 6597916 (99.92%) read pairs available; of these: 1264847 (19.17%) trimmed read pairs available after processing 5333069 (80.83%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 7 0.00% 20 12 0.00% 21 13 0.00% 22 8 0.00% 23 6 0.00% 24 8 0.00% 25 11 0.00% 26 17 0.00% 27 20 0.00% 28 14 0.00% 29 11 0.00% 30 15 0.00% 31 26 0.00% 32 19 0.00% 33 11 0.00% 34 13 0.00% 35 21 0.00% 36 15 0.00% 37 18 0.00% 38 28 0.00% 39 20 0.00% 40 22 0.00% 41 31 0.00% 42 19 0.00% 43 25 0.00% 44 31 0.00% 45 28 0.00% 46 51 0.00% 47 46 0.00% 48 39 0.00% 49 44 0.00% 50 54 0.00% 51 65 0.00% 52 65 0.00% 53 59 0.00% 54 71 0.00% 55 73 0.00% 56 94 0.00% 57 94 0.00% 58 131 0.00% 59 122 0.00% 60 143 0.00% 61 151 0.00% 62 148 0.00% 63 176 0.00% 64 167 0.00% 65 189 0.00% 66 213 0.00% 67 211 0.00% 68 229 0.00% 69 278 0.00% 70 297 0.00% 71 294 0.00% 72 360 0.01% 73 340 0.01% 74 405 0.01% 75 435 0.01% 76 462 0.01% 77 557 0.01% 78 557 0.01% 79 633 0.01% 80 636 0.01% 81 722 0.01% 82 882 0.01% 83 785 0.01% 84 973 0.01% 85 1195 0.02% 86 1268 0.02% 87 1446 0.02% 88 1418 0.02% 89 1348 0.02% 90 1395 0.02% 91 1538 0.02% 92 1554 0.02% 93 1671 0.03% 94 1619 0.02% 95 1815 0.03% 96 2132 0.03% 97 1924 0.03% 98 1957 0.03% 99 2104 0.03% 100 2086 0.03% 101 2164 0.03% 102 1993 0.03% 103 2022 0.03% 104 2187 0.03% 105 2362 0.04% 106 2193 0.03% 107 2176 0.03% 108 2205 0.03% 109 2182 0.03% 110 2219 0.03% 111 2469 0.04% 112 2610 0.04% 113 2412 0.04% 114 2690 0.04% 115 2938 0.04% 116 2863 0.04% 117 2951 0.04% 118 3116 0.05% 119 3083 0.05% 120 3688 0.06% 121 3092 0.05% 122 3552 0.05% 123 4180 0.06% 124 3895 0.06% 125 3803 0.06% 126 3921 0.06% 127 3903 0.06% 128 3882 0.06% 129 4322 0.07% 130 4478 0.07% 131 4576 0.07% 132 4832 0.07% 133 4947 0.07% 134 4914 0.07% 135 5101 0.08% 136 5571 0.08% 137 5584 0.08% 138 5817 0.09% 139 6233 0.09% 140 6474 0.10% 141 7066 0.11% 142 7775 0.12% 143 8699 0.13% 144 10595 0.16% 145 12750 0.19% 146 15443 0.23% 147 22121 0.34% 148 36345 0.55% 149 84516 1.28% 150 877744 13.30% 151 5333069 80.83% 6597916 reads passed initial QC criterion=sequence-density sequence-density=2.42 sequence-density-rank=1 fanout-score=1.70 fanout-score-rank=30 prefix-density=4.10 prefix-fanout=1.0 sequence=CGACGGTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGATCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTTGCCCCGCTTCCGACCCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTAT criterion=fanout-score sequence-density=0.05 sequence-density-rank=26 fanout-score=84.60 fanout-score-rank=1 prefix-density=4.43 prefix-fanout=1.0 sequence=CAATCCAACACCTGGTGAATTCT criterion=sequence-density sequence-density=0.66 sequence-density-rank=1 fanout-score=2.49 fanout-score-rank=20 prefix-density=1.64 prefix-fanout=1.0 sequence=GAGAAACGGCTGCCACATCCAAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=114.09 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=3.0 sequence=GCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGC SRR6031183 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 00:41:24 Started mapping on | Dec 10 00:41:25 Finished on | Dec 10 00:42:31 Mapping speed, Million of reads per hour | 359.89 Number of input reads | 6597916 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 2056947 Uniquely mapped reads % | 31.18% Average mapped length | 298.99 Number of splices: Total | 2181265 Number of splices: Annotated (sjdb) | 2075141 Number of splices: GT/AG | 2152384 Number of splices: GC/AG | 25427 Number of splices: AT/AC | 1557 Number of splices: Non-canonical | 1897 Mismatch rate per base, % | 0.08% Deletion rate per base | 0.01% Deletion average length | 1.47 Insertion rate per base | 0.00% Insertion average length | 1.20 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 30453 % of reads mapped to multiple loci | 0.46% Number of reads mapped to too many loci | 461188 % of reads mapped to too many loci | 6.99% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.04% % of reads unmapped: other | 56.33% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4511323 4511323 4511323 N_multimapping 30453 30453 30453 N_noFeature 141382 1999178 161683 N_ambiguous 52760 509 15350 UnstrandedReadsAssigned:1862805 PositiveStrandReadsAssigned:57260 NegativeStrandReadsAssigned:1879914 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6031183 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6031183-trimmed-pair1.fastq SRR6031183-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 6,597,916 reads, 2,042,302 reads pseudoaligned [quant] estimated average fragment length: 431.03 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,023 rounds 52973 SRR6031183.ke.tsv 35125 SRR6031183.se.tsv 88098 total ==> SRR6031183.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 507.464 0 0 PNS24247 1044 613.97 11.4526 13.3783 PNS24249 1928 1497.97 7.65725 3.66619 PNS24246 1044 613.97 11.4526 13.3783 PNS24248 1044 613.97 11.4526 13.3783 PNS24244 1471 1040.97 11.985 8.25742 PNS24243 293 67.9814 0 0 KQK14069 1603 1172.97 456.022 278.833 KQK14071 474 138.335 2.77554 14.39 ==> SRR6031183.se.tsv <== BRADI_1g14170v3 444 BRADI_1g53295v3 16 BRADI_1g59795v3 55 BRADI_1g07683v3 0 BRADI_1g00485v3 7 BRADI_1g20270v3 102 BRADI_1g74790v3 21 BRADI_1g09890v3 0 BRADI_1g77505v3 28 BRADI_1g48960v3 0 SRR6031183 completed mapping pipeline successfully