Starting /dee2/code/volunteer_pipeline.sh SRR6127921 current disk space = 1526338695168 free memory = 1599066272 SRR6127921 SRAfilesize 7deff5ef3ea197e5ef597d85188ecf60 SRR6127921.sra SRR6127921.sra file validated SRR6127921 is paired end SRR6127921 is conventional basespace SRR6127921 read1 length is 100 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6127921_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.367 33.0 33.0 33.0 33.0 33.0 2 32.4965 33.0 33.0 33.0 33.0 33.0 3 32.72125 33.0 33.0 33.0 33.0 33.0 4 36.72875 37.0 37.0 37.0 37.0 37.0 5 36.703 37.0 37.0 37.0 37.0 37.0 6 36.673 37.0 37.0 37.0 37.0 37.0 7 36.58725 37.0 37.0 37.0 37.0 37.0 8 36.65 37.0 37.0 37.0 37.0 37.0 9 36.65125 37.0 37.0 37.0 37.0 37.0 10-11 36.651375 37.0 37.0 37.0 37.0 37.0 12-13 36.58725 37.0 37.0 37.0 37.0 37.0 14-15 38.924875 40.0 40.0 40.0 37.0 40.0 16-17 38.94825 40.0 40.0 40.0 37.0 40.0 18-19 38.947625 40.0 40.0 40.0 37.0 40.0 20-21 38.8725 40.0 40.0 40.0 37.0 40.0 22-23 38.795249999999996 40.0 40.0 40.0 37.0 40.0 24-25 38.739125 40.0 38.5 40.0 37.0 40.0 26-27 38.702625 40.0 37.0 40.0 37.0 40.0 28-29 38.635000000000005 40.0 37.0 40.0 37.0 40.0 30-31 38.627624999999995 40.0 37.0 40.0 37.0 40.0 32-33 38.600875 40.0 37.0 40.0 37.0 40.0 34-35 38.481750000000005 40.0 37.0 40.0 37.0 40.0 36-37 38.403499999999994 40.0 37.0 40.0 37.0 40.0 38-39 38.219875 40.0 37.0 40.0 37.0 40.0 40-41 38.106375 40.0 37.0 40.0 33.0 40.0 42-43 37.885999999999996 40.0 37.0 40.0 33.0 40.0 44-45 37.84 40.0 37.0 40.0 33.0 40.0 46-47 37.651375 40.0 37.0 40.0 33.0 40.0 48-49 37.596374999999995 40.0 37.0 40.0 33.0 40.0 50-51 37.418000000000006 40.0 37.0 40.0 33.0 40.0 52-53 37.060874999999996 38.5 37.0 40.0 33.0 40.0 54-55 36.6695 37.0 37.0 40.0 33.0 40.0 56-57 36.575625 37.0 37.0 40.0 33.0 40.0 58-59 36.37975 37.0 37.0 40.0 33.0 40.0 60-61 36.100375 37.0 37.0 40.0 33.0 40.0 62-63 35.816 37.0 37.0 40.0 33.0 40.0 64-65 35.697374999999994 37.0 37.0 38.5 33.0 40.0 66-67 35.326 37.0 35.0 37.0 33.0 40.0 68-69 35.14275 37.0 33.0 37.0 33.0 40.0 70-71 34.835499999999996 37.0 33.0 37.0 30.0 40.0 72-73 34.557500000000005 37.0 33.0 37.0 27.0 40.0 74-75 34.06725 37.0 33.0 37.0 27.0 37.0 76-77 31.53775 33.0 30.0 35.0 24.5 37.0 78-79 33.316 37.0 33.0 37.0 27.0 37.0 80-81 33.42575 37.0 33.0 37.0 27.0 37.0 82-83 33.529624999999996 37.0 33.0 37.0 27.0 37.0 84-85 33.505375 37.0 33.0 37.0 27.0 37.0 86-87 33.420500000000004 37.0 33.0 37.0 27.0 37.0 88-89 33.289125 37.0 33.0 37.0 27.0 37.0 90-91 33.372125 37.0 33.0 37.0 27.0 37.0 92-93 33.1745 37.0 33.0 37.0 27.0 37.0 94-95 33.060625 37.0 33.0 37.0 27.0 37.0 96-97 32.615750000000006 37.0 33.0 37.0 24.5 37.0 98-99 32.286375 37.0 33.0 37.0 22.0 37.0 100 28.616 33.0 27.0 33.0 15.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 2.0 11 5.0 12 4.0 13 3.0 14 5.0 15 5.0 16 4.0 17 9.0 18 7.0 19 8.0 20 15.0 21 9.0 22 9.0 23 10.0 24 17.0 25 27.0 26 23.0 27 33.0 28 31.0 29 39.0 30 49.0 31 72.0 32 95.0 33 137.0 34 152.0 35 300.0 36 547.0 37 1407.0 38 973.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.066312325993415 10.275879524171096 8.706656542647432 42.95115160718805 2 23.599999999999998 14.299999999999999 36.6 25.5 3 23.849999999999998 20.474999999999998 23.45 32.225 4 26.450000000000003 28.925 23.400000000000002 21.224999999999998 5 25.974999999999998 30.95 24.125 18.95 6 20.225 34.55 26.674999999999997 18.55 7 15.275 23.125 42.775 18.825 8 19.8 21.65 31.175000000000004 27.375 9 20.674999999999997 20.974999999999998 34.075 24.275 10-11 24.1625 31.0 22.7625 22.075 12-13 22.925 24.337500000000002 27.525 25.2125 14-15 22.25 26.3625 26.737499999999997 24.65 16-17 23.2375 25.687500000000004 26.150000000000002 24.925 18-19 23.45 25.95 26.4625 24.1375 20-21 24.05 26.2125 26.237500000000004 23.5 22-23 21.7 26.4125 26.275 25.6125 24-25 22.787499999999998 25.074999999999996 26.8125 25.324999999999996 26-27 22.912499999999998 26.087500000000002 27.925 23.075000000000003 28-29 22.8875 25.900000000000002 27.800000000000004 23.4125 30-31 22.0625 27.3625 26.6625 23.9125 32-33 22.95 27.487499999999997 26.174999999999997 23.3875 34-35 22.4375 25.95 26.937499999999996 24.675 36-37 23.599999999999998 25.4625 26.4125 24.525 38-39 23.6125 25.874999999999996 25.0 25.5125 40-41 23.974999999999998 25.8 26.75 23.474999999999998 42-43 25.0125 25.825 25.7625 23.400000000000002 44-45 24.5625 25.4375 25.9625 24.0375 46-47 23.825 26.6 25.95 23.625 48-49 24.275 25.6125 26.0125 24.099999999999998 50-51 24.2625 24.675 27.1 23.962500000000002 52-53 23.7 24.75 25.874999999999996 25.674999999999997 54-55 24.675 25.1 26.575 23.65 56-57 22.900000000000002 25.4375 26.825 24.837500000000002 58-59 23.3375 26.224999999999998 25.624999999999996 24.8125 60-61 22.7125 25.85 26.25 25.1875 62-63 23.0625 25.9875 28.075 22.875 64-65 21.6125 27.462500000000002 26.275 24.65 66-67 23.0125 26.05 25.874999999999996 25.0625 68-69 23.425 25.525 25.8125 25.2375 70-71 24.2625 25.924999999999997 26.4625 23.35 72-73 24.825 24.887500000000003 25.362499999999997 24.925 74-75 24.3 25.424999999999997 25.624999999999996 24.65 76-77 24.3 25.687500000000004 25.6 24.4125 78-79 23.0875 26.075 25.9875 24.85 80-81 23.8125 25.674999999999997 26.337500000000002 24.175 82-83 24.1625 25.45 26.3625 24.025 84-85 23.8625 25.974999999999998 25.8125 24.349999999999998 86-87 24.3125 25.112499999999997 24.975 25.6 88-89 23.75 25.162499999999998 26.0 25.087500000000002 90-91 23.8375 24.837500000000002 25.775 25.55 92-93 23.05 26.150000000000002 26.3625 24.4375 94-95 23.25 25.412499999999998 26.937499999999996 24.4 96-97 24.2625 25.5375 25.7375 24.462500000000002 98-99 23.474999999999998 25.575 26.025 24.925 100 24.55 24.95 24.075 26.424999999999997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.5 24 0.5 25 0.5 26 1.5 27 1.5 28 4.0 29 12.5 30 21.0 31 27.5 32 34.0 33 43.5 34 49.0 35 59.5 36 74.0 37 88.5 38 112.0 39 106.5 40 88.0 41 101.0 42 121.0 43 143.0 44 172.0 45 177.0 46 180.0 47 194.5 48 215.5 49 191.5 50 154.5 51 166.0 52 194.0 53 190.0 54 150.0 55 135.5 56 114.5 57 89.5 58 83.0 59 86.5 60 79.0 61 59.5 62 50.5 63 48.5 64 41.0 65 25.5 66 17.0 67 18.5 68 20.5 69 15.5 70 9.0 71 4.5 72 5.5 73 6.5 74 4.5 75 2.5 76 2.0 77 2.5 78 1.0 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.225 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.325 #Duplication Level Percentage of deduplicated Percentage of total 1 85.91549295774648 65.575 2 7.664592204389126 11.700000000000001 3 2.6203734032099577 6.0 4 1.5722240419259743 4.8 5 0.7206026858827383 2.75 6 0.491320013101867 2.25 7 0.22928267278087125 1.225 8 0.26203734032099574 1.6 9 0.2947920078611202 2.025 >10 0.22928267278087125 2.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 14 0.35000000000000003 No Hit CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA 13 0.325 No Hit GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC 12 0.3 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 12 0.3 No Hit CAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGC 12 0.3 No Hit CCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCC 10 0.25 No Hit CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA 10 0.25 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 9 0.22499999999999998 No Hit GTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCG 9 0.22499999999999998 No Hit CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC 9 0.22499999999999998 No Hit GGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTT 9 0.22499999999999998 No Hit GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT 9 0.22499999999999998 No Hit CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAA 9 0.22499999999999998 No Hit CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT 9 0.22499999999999998 No Hit GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA 9 0.22499999999999998 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 9 0.22499999999999998 No Hit CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT 8 0.2 No Hit GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT 8 0.2 No Hit GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG 8 0.2 No Hit GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT 8 0.2 No Hit GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA 8 0.2 No Hit CCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGC 8 0.2 No Hit GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT 8 0.2 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 8 0.2 No Hit CCGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCC 7 0.17500000000000002 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 7 0.17500000000000002 No Hit GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA 7 0.17500000000000002 No Hit GTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTT 7 0.17500000000000002 No Hit CTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTT 7 0.17500000000000002 No Hit CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT 7 0.17500000000000002 No Hit GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG 7 0.17500000000000002 No Hit CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG 6 0.15 No Hit GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC 6 0.15 No Hit GCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCC 6 0.15 No Hit CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA 6 0.15 No Hit GTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTG 6 0.15 No Hit ATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACG 6 0.15 No Hit ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT 6 0.15 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 6 0.15 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6 0.15 No Hit CCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGT 6 0.15 No Hit GTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCG 6 0.15 No Hit ATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGG 6 0.15 No Hit TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT 6 0.15 No Hit GCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAAT 6 0.15 No Hit GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC 6 0.15 No Hit CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC 5 0.125 No Hit GCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGAC 5 0.125 No Hit CTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAG 5 0.125 No Hit AATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGATC 5 0.125 No Hit CTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTC 5 0.125 No Hit GGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAG 5 0.125 No Hit CAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTC 5 0.125 No Hit GATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGG 5 0.125 No Hit CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT 5 0.125 No Hit CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT 5 0.125 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 5 0.125 No Hit GCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAG 5 0.125 No Hit CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA 5 0.125 No Hit GTGTGGCACCAGCGCGGCTACTCCGATAGCGACTGGACACAACATGGCAG 5 0.125 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 5 0.125 No Hit AGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTATATT 5 0.125 No Hit CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG 5 0.125 No Hit GCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCG 5 0.125 No Hit GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC 5 0.125 No Hit GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC 5 0.125 No Hit GTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTCCGC 5 0.125 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.16249999999999998 0.0 0.0 0.0 0.0 74-75 0.21250000000000002 0.0 0.0 0.0 0.0 76-77 0.2875 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.425 0.0 0.0 0.0 0.0 82-83 0.5 0.0 0.0 0.0 0.0 84-85 0.6875 0.0 0.0 0.0 0.0 86-87 0.8875 0.0 0.0 0.0 0.0 88 1.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATTTC 25 0.004866334 56.392498 3 TTGATTT 25 0.004866334 56.392498 2 >>END_MODULE SRR6127921 read2 length is 101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6127921_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85675 33.0 33.0 33.0 33.0 33.0 2 32.00325 33.0 33.0 33.0 33.0 33.0 3 32.00625 33.0 33.0 33.0 33.0 33.0 4 35.56625 37.0 37.0 37.0 33.0 37.0 5 35.45275 37.0 37.0 37.0 33.0 37.0 6 35.4105 37.0 37.0 37.0 33.0 37.0 7 35.5015 37.0 37.0 37.0 33.0 37.0 8 35.5955 37.0 37.0 37.0 33.0 37.0 9 35.89975 37.0 37.0 37.0 37.0 37.0 10-11 36.02312499999999 37.0 37.0 37.0 37.0 37.0 12-13 35.996624999999995 37.0 37.0 37.0 37.0 37.0 14-15 38.206375 40.0 37.0 40.0 37.0 40.0 16-17 38.142250000000004 40.0 37.0 40.0 37.0 40.0 18-19 38.192375 40.0 37.0 40.0 37.0 40.0 20-21 38.179 40.0 38.5 40.0 37.0 40.0 22-23 37.532875000000004 40.0 37.0 40.0 33.0 40.0 24-25 37.94375 40.0 37.0 40.0 37.0 40.0 26-27 38.006125 40.0 37.0 40.0 37.0 40.0 28-29 38.075625 40.0 37.0 40.0 37.0 40.0 30-31 38.01375 40.0 37.0 40.0 37.0 40.0 32-33 37.83175 40.0 37.0 40.0 37.0 40.0 34-35 37.937375 40.0 37.0 40.0 37.0 40.0 36-37 37.762375 40.0 37.0 40.0 33.0 40.0 38-39 37.689 40.0 37.0 40.0 33.0 40.0 40-41 37.60275 40.0 37.0 40.0 33.0 40.0 42-43 37.443875000000006 40.0 37.0 40.0 33.0 40.0 44-45 37.1815 40.0 37.0 40.0 33.0 40.0 46-47 37.040125 40.0 37.0 40.0 33.0 40.0 48-49 36.89425 40.0 37.0 40.0 33.0 40.0 50-51 35.169375 38.5 35.0 38.5 30.0 40.0 52-53 35.514375 37.0 35.0 38.5 33.0 40.0 54-55 36.277 37.0 37.0 40.0 33.0 40.0 56-57 36.18675 37.0 37.0 40.0 33.0 40.0 58-59 35.93875 37.0 37.0 40.0 33.0 40.0 60-61 35.517250000000004 37.0 37.0 40.0 33.0 40.0 62-63 35.469 37.0 37.0 37.0 33.0 40.0 64-65 35.25425 37.0 37.0 37.0 33.0 40.0 66-67 35.111125 37.0 37.0 37.0 33.0 40.0 68-69 34.907375 37.0 35.0 37.0 33.0 40.0 70-71 34.78 37.0 33.0 37.0 33.0 40.0 72-73 34.573125 37.0 33.0 37.0 30.0 38.5 74-75 34.416125 37.0 33.0 37.0 30.0 37.0 76-77 34.158875 37.0 33.0 37.0 27.0 37.0 78-79 33.855625 37.0 33.0 37.0 27.0 37.0 80-81 33.716375 37.0 33.0 37.0 27.0 37.0 82-83 33.664375 37.0 33.0 37.0 27.0 37.0 84-85 33.626000000000005 37.0 33.0 37.0 27.0 37.0 86-87 33.616875 37.0 33.0 37.0 27.0 37.0 88-89 33.4845 37.0 33.0 37.0 27.0 37.0 90-91 33.507875 37.0 33.0 37.0 27.0 37.0 92-93 33.348749999999995 37.0 33.0 37.0 27.0 37.0 94-95 33.194374999999994 37.0 33.0 37.0 27.0 37.0 96-97 32.936375 37.0 33.0 37.0 27.0 37.0 98-99 32.9045 37.0 33.0 37.0 27.0 37.0 100-101 31.2555 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54.0 3 2.0 4 1.0 5 4.0 6 4.0 7 2.0 8 1.0 9 4.0 10 2.0 11 4.0 12 1.0 13 9.0 14 6.0 15 5.0 16 4.0 17 5.0 18 4.0 19 4.0 20 12.0 21 7.0 22 11.0 23 15.0 24 14.0 25 16.0 26 22.0 27 28.0 28 21.0 29 39.0 30 38.0 31 59.0 32 96.0 33 109.0 34 146.0 35 232.0 36 499.0 37 1583.0 38 937.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.625 17.424999999999997 11.450000000000001 37.5 2 26.875 24.825 30.625000000000004 17.675 3 21.9 27.35 26.6 24.15 4 26.5 32.45 19.575 21.475 5 26.575 33.6 19.400000000000002 20.424999999999997 6 21.65 36.925000000000004 20.625 20.8 7 20.674999999999997 18.5 36.1 24.725 8 22.025 23.95 24.725 29.299999999999997 9 25.775 23.3 25.900000000000002 25.025 10-11 25.324999999999996 29.9875 19.9375 24.75 12-13 25.0 24.4125 24.0125 26.575 14-15 24.2 27.075 24.975 23.75 16-17 25.337500000000002 26.450000000000003 23.925 24.2875 18-19 25.0625 27.05 23.5 24.3875 20-21 25.550275137568786 26.713356678339167 23.58679339669835 24.149574787393696 22-23 25.4375 26.325 24.325 23.9125 24-25 24.72809101137642 26.22827853481685 24.090511313914238 24.953119139892486 26-27 25.924999999999997 27.125 22.9375 24.0125 28-29 25.2 26.637499999999996 24.5625 23.599999999999998 30-31 25.55 26.174999999999997 24.2375 24.0375 32-33 25.224999999999998 26.200000000000003 24.8125 23.7625 34-35 25.112499999999997 26.5625 24.887500000000003 23.4375 36-37 25.650000000000002 24.5625 25.2875 24.5 38-39 24.9125 26.7125 24.1125 24.2625 40-41 25.387500000000003 25.674999999999997 25.0375 23.9 42-43 25.124999999999996 26.3625 24.375 24.1375 44-45 25.224999999999998 26.1625 24.962500000000002 23.65 46-47 24.8625 26.3125 25.412499999999998 23.4125 48-49 24.5625 25.15 25.650000000000002 24.637500000000003 50-51 24.712500000000002 26.775 25.637500000000003 22.875 52-53 25.162499999999998 26.3 24.0125 24.525 54-55 25.35 26.674999999999997 24.925 23.05 56-57 24.712500000000002 25.825 25.8 23.6625 58-59 25.6 26.525 25.324999999999996 22.55 60-61 24.725 25.9625 25.124999999999996 24.1875 62-63 24.762500000000003 25.937500000000004 25.6125 23.6875 64-65 25.2125 27.037499999999998 24.637500000000003 23.1125 66-67 24.5125 25.4 25.2 24.887500000000003 68-69 24.615576947118388 26.303287910988875 25.790723840480062 23.29041130141268 70-71 25.2625 25.5625 25.3125 23.8625 72-73 23.502937867233403 25.415676959619955 26.24078009751219 24.840605075634453 74-75 24.5125 25.887500000000003 25.3 24.3 76-77 24.4375 27.212500000000002 24.5625 23.7875 78-79 23.846442415905962 27.335250719019633 24.534200325121923 24.284106539952482 80-81 24.275 27.537499999999998 25.0625 23.125 82-83 25.700350175087543 26.76338169084542 24.112056028014006 23.424212106053027 84-85 24.111166750125186 27.666499749624435 24.624436654982475 23.5978968452679 86-87 24.49056132016502 25.86573321665208 25.778222277784725 23.865483185398176 88-89 25.2625 27.725 24.3625 22.650000000000002 90-91 25.5125 28.462500000000002 24.125 21.9 92-93 24.375 26.150000000000002 25.412499999999998 24.0625 94-95 25.275 26.974999999999998 25.35 22.400000000000002 96-97 25.35 26.575 25.874999999999996 22.2 98-99 24.224999999999998 27.6625 24.975 23.1375 100-101 25.662499999999998 26.575 24.2 23.5625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.5 24 1.0 25 1.0 26 2.5 27 4.0 28 7.0 29 11.0 30 14.5 31 25.0 32 39.0 33 37.0 34 40.5 35 59.5 36 73.0 37 74.0 38 95.0 39 114.5 40 115.0 41 103.0 42 93.5 43 110.5 44 130.5 45 148.0 46 148.5 47 176.5 48 205.0 49 196.0 50 176.5 51 169.5 52 167.0 53 167.0 54 165.5 55 159.5 56 142.5 57 109.0 58 113.5 59 119.5 60 100.5 61 76.0 62 57.5 63 47.5 64 34.5 65 30.5 66 30.5 67 23.0 68 14.5 69 12.5 70 10.5 71 9.5 72 8.5 73 7.5 74 5.5 75 1.5 76 2.0 77 1.0 78 0.5 79 1.0 80 1.0 81 3.0 82 2.5 83 0.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.05 22-23 0.0 24-25 0.0125 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0125 70-71 0.0 72-73 0.0125 74-75 0.0 76-77 0.0 78-79 0.0375 80-81 0.0 82-83 0.05 84-85 0.15 86-87 0.0125 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.875 #Duplication Level Percentage of deduplicated Percentage of total 1 85.95879556259905 67.80000000000001 2 7.9873217115689386 12.6 3 3.0427892234548337 7.199999999999999 4 1.5213946117274169 4.8 5 0.5705229793977813 2.25 6 0.4437400950871633 2.1 7 0.22187004754358164 1.225 8 0.1901743264659271 1.2 9 0.0 0.0 >10 0.06339144215530904 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 20 0.5 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 13 0.325 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 8 0.2 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 8 0.2 No Hit CTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATGTTATTAACCA 8 0.2 No Hit CGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAA 8 0.2 No Hit GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA 8 0.2 No Hit AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC 8 0.2 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 7 0.17500000000000002 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 7 0.17500000000000002 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 7 0.17500000000000002 No Hit CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG 7 0.17500000000000002 No Hit CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA 7 0.17500000000000002 No Hit CAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAG 7 0.17500000000000002 No Hit GCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACA 7 0.17500000000000002 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 6 0.15 No Hit GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC 6 0.15 No Hit CTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAG 6 0.15 No Hit CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC 6 0.15 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 6 0.15 No Hit GTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGA 6 0.15 No Hit ATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGG 6 0.15 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 6 0.15 No Hit GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA 6 0.15 No Hit CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC 6 0.15 No Hit GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT 6 0.15 No Hit CATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTG 6 0.15 No Hit GGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTG 6 0.15 No Hit CAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGT 6 0.15 No Hit CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATT 5 0.125 No Hit CGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGAT 5 0.125 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 5 0.125 No Hit CGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATG 5 0.125 No Hit GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG 5 0.125 No Hit GGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCA 5 0.125 No Hit GGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATG 5 0.125 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 5 0.125 No Hit CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC 5 0.125 No Hit GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA 5 0.125 No Hit GAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAA 5 0.125 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 5 0.125 No Hit CCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTG 5 0.125 No Hit CTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAAT 5 0.125 No Hit CGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGT 5 0.125 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 5 0.125 No Hit GCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTG 5 0.125 No Hit CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.16249999999999998 0.0 0.0 0.0 0.0 74-75 0.1875 0.0 0.0 0.0 0.0 76-77 0.2625 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.475 0.0 0.0 0.0 0.0 84-85 0.6625 0.0 0.0 0.0 0.0 86-87 0.8625 0.0 0.0 0.0 0.0 88-89 1.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628824 spots for SRR6127921.sra Written 1628824 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra Read 1628823 spots for SRR6127921.sra Written 1628823 spots for SRR6127921.sra SRR ids: ['SRR6127921.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_9d5zyzc3 SRR6127921.sra spots: 32576461 blocks: [[1, 1628823], [1628824, 3257646], [3257647, 4886469], [4886470, 6515292], [6515293, 8144115], [8144116, 9772938], [9772939, 11401761], [11401762, 13030584], [13030585, 14659407], [14659408, 16288230], [16288231, 17917053], [17917054, 19545876], [19545877, 21174699], [21174700, 22803522], [22803523, 24432345], [24432346, 26061168], [26061169, 27689991], [27689992, 29318814], [29318815, 30947637], [30947638, 32576461]] SRR6127921 file size 7772472 SRR6127921 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6127921 SRR6127921_1.fastq SRR6127921_2.fastq Input file: SRR6127921_1.fastq Paired file: SRR6127921_2.fastq trimmed: SRR6127921-trimmed-pair1.fastq, SRR6127921-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 04:18:47 2024 >> started Tue Dec 10 04:19:23 2024 >> done (36.284s) 32576461 read pairs processed; of these: 121613 ( 0.37%) short read pairs filtered out after trimming by size control 342783 ( 1.05%) empty read pairs filtered out after trimming by size control 32112065 (98.57%) read pairs available; of these: 4816139 (15.00%) trimmed read pairs available after processing 27295926 (85.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 22 0.00% 20 25 0.00% 21 43 0.00% 22 94 0.00% 23 121 0.00% 24 191 0.00% 25 221 0.00% 26 297 0.00% 27 371 0.00% 28 496 0.00% 29 554 0.00% 30 672 0.00% 31 764 0.00% 32 884 0.00% 33 1114 0.00% 34 1296 0.00% 35 1459 0.00% 36 1653 0.01% 37 1775 0.01% 38 1946 0.01% 39 2221 0.01% 40 2487 0.01% 41 2791 0.01% 42 3093 0.01% 43 3398 0.01% 44 3740 0.01% 45 4088 0.01% 46 4588 0.01% 47 4798 0.01% 48 5323 0.02% 49 5507 0.02% 50 5938 0.02% 51 6452 0.02% 52 6927 0.02% 53 7540 0.02% 54 7958 0.02% 55 8775 0.03% 56 9451 0.03% 57 10400 0.03% 58 11458 0.04% 59 21151 0.07% 60 22438 0.07% 61 24025 0.07% 62 27212 0.08% 63 29055 0.09% 64 30659 0.10% 65 33192 0.10% 66 33245 0.10% 67 34179 0.11% 68 35554 0.11% 69 38688 0.12% 70 39563 0.12% 71 41327 0.13% 72 44012 0.14% 73 44819 0.14% 74 46950 0.15% 75 42743 0.13% 76 41752 0.13% 77 46363 0.14% 78 50168 0.16% 79 53340 0.17% 80 56773 0.18% 81 63732 0.20% 82 65764 0.20% 83 71386 0.22% 84 77314 0.24% 85 87433 0.27% 86 109169 0.34% 87 105037 0.33% 88 96831 0.30% 89 95084 0.30% 90 107892 0.34% 91 117248 0.37% 92 132259 0.41% 93 144721 0.45% 94 173338 0.54% 95 201961 0.63% 96 250455 0.78% 97 323242 1.01% 98 414911 1.29% 99 538819 1.68% 100 27967322 87.09% 32112065 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=1.94 fanout-score-rank=31 prefix-density=0.38 prefix-fanout=1.9 sequence=TTCAAATGTACA criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=55.33 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=1.2 sequence=TCAGAAATCAGAACGATCATGTTTCTTCTGCCCTTGATAATTTACACGTACGGTACATTCGGACTCTCGTACACTTTCCCGGTAAAATTAACACAGCACATCATGCTGCATTGCACGCACGCATGCAGCTGATCATCGACAAGTCGACGGCAATTGCGATGAAGAGCTCACAGGCTGGGCGTCCTCTCGGCAGCTCCGGAGATGACGGTGAGCAGGTTGTTGCCGAAGGGGTCGCTGAGGTGCGCAGAGAGGTTCTCAACGGGGCCCTCGCCAGTGACGTATGCCTGGATGAA criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=3.29 fanout-score-rank=14 prefix-density=0.45 prefix-fanout=2.4 sequence=CCATGTTCGGGTGCACCGACGCCAC criterion=fanout-score sequence-density=0.11 sequence-density-rank=21 fanout-score=12.50 fanout-score-rank=1 prefix-density=1.38 prefix-fanout=1.0 sequence=ACATGAAAAAAACAGTG SRR6127921 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 04:20:31 Started mapping on | Dec 10 04:20:32 Finished on | Dec 10 04:57:18 Mapping speed, Million of reads per hour | 52.40 Number of input reads | 32112065 Average input read length | 197 UNIQUE READS: Uniquely mapped reads number | 10916767 Uniquely mapped reads % | 34.00% Average mapped length | 196.74 Number of splices: Total | 4668326 Number of splices: Annotated (sjdb) | 4332405 Number of splices: GT/AG | 4596351 Number of splices: GC/AG | 54706 Number of splices: AT/AC | 1046 Number of splices: Non-canonical | 16223 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.38 Insertion rate per base | 0.03% Insertion average length | 1.98 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 639656 % of reads mapped to multiple loci | 1.99% Number of reads mapped to too many loci | 83419 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 62.35% % of reads unmapped: other | 1.41% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 20563847 20563847 20563847 N_multimapping 639656 639656 639656 N_noFeature 474750 10559713 545129 N_ambiguous 327904 977 42234 UnstrandedReadsAssigned:10114113 PositiveStrandReadsAssigned:356077 NegativeStrandReadsAssigned:10329404 Dataset is classified negative stranded MeadianReadLen=100 20thPercentileLength=100 echo kmer=95 SRR6127921 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6127921-trimmed-pair1.fastq SRR6127921-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,112,065 reads, 10,439,855 reads pseudoaligned [quant] estimated average fragment length: 164.183 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,114 rounds 52973 SRR6127921.ke.tsv 35125 SRR6127921.se.tsv 88098 total ==> SRR6127921.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 772.864 0 0 PNS24247 1044 880.817 11.2607 1.56132 PNS24249 1928 1764.82 0 0 PNS24246 1044 880.817 11.2607 1.56132 PNS24248 1044 880.817 11.2607 1.56132 PNS24244 1471 1307.82 141.218 13.1873 PNS24243 293 135.229 3 2.70935 KQK14069 1603 1439.82 1274.77 108.128 KQK14071 474 311.738 39.8388 15.6074 ==> SRR6127921.se.tsv <== BRADI_1g14170v3 1521 BRADI_1g53295v3 301 BRADI_1g59795v3 146 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 103 BRADI_1g74790v3 32 BRADI_1g09890v3 0 BRADI_1g77505v3 208 BRADI_1g48960v3 0 SRR6127921 completed mapping pipeline successfully