Starting /dee2/code/volunteer_pipeline.sh SRR6127945 current disk space = 1526065029120 free memory = 1598517600 SRR6127945 SRAfilesize 86dc889e7c5dfba7341e93898008f52c SRR6127945.sra SRR6127945.sra file validated SRR6127945 is paired end SRR6127945 is conventional basespace SRR6127945 read1 length is 100 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6127945_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37825 33.0 33.0 33.0 33.0 33.0 2 32.545 33.0 33.0 33.0 33.0 33.0 3 32.7545 33.0 33.0 33.0 33.0 33.0 4 36.735 37.0 37.0 37.0 37.0 37.0 5 36.732 37.0 37.0 37.0 37.0 37.0 6 36.68575 37.0 37.0 37.0 37.0 37.0 7 36.61525 37.0 37.0 37.0 37.0 37.0 8 36.6685 37.0 37.0 37.0 37.0 37.0 9 36.67725 37.0 37.0 37.0 37.0 37.0 10-11 36.644125 37.0 37.0 37.0 37.0 37.0 12-13 36.61225 37.0 37.0 37.0 37.0 37.0 14-15 38.914875 40.0 40.0 40.0 37.0 40.0 16-17 38.949875 40.0 40.0 40.0 37.0 40.0 18-19 38.911500000000004 40.0 40.0 40.0 37.0 40.0 20-21 38.899249999999995 40.0 40.0 40.0 37.0 40.0 22-23 38.829875 40.0 38.5 40.0 37.0 40.0 24-25 38.719125 40.0 37.0 40.0 37.0 40.0 26-27 38.622875 40.0 37.0 40.0 37.0 40.0 28-29 38.5965 40.0 37.0 40.0 37.0 40.0 30-31 38.59375 40.0 37.0 40.0 37.0 40.0 32-33 38.554 40.0 37.0 40.0 37.0 40.0 34-35 38.481875 40.0 37.0 40.0 37.0 40.0 36-37 38.316125 40.0 37.0 40.0 37.0 40.0 38-39 38.096375 40.0 37.0 40.0 35.0 40.0 40-41 38.02475 40.0 37.0 40.0 33.0 40.0 42-43 37.795500000000004 40.0 37.0 40.0 33.0 40.0 44-45 37.69725 40.0 37.0 40.0 33.0 40.0 46-47 37.473625 40.0 37.0 40.0 33.0 40.0 48-49 37.387 40.0 37.0 40.0 33.0 40.0 50-51 37.172375 40.0 37.0 40.0 33.0 40.0 52-53 36.850625 37.0 37.0 40.0 33.0 40.0 54-55 36.312375 37.0 37.0 40.0 33.0 40.0 56-57 36.353875 37.0 37.0 40.0 33.0 40.0 58-59 36.179 37.0 37.0 40.0 33.0 40.0 60-61 35.908125 37.0 37.0 40.0 33.0 40.0 62-63 35.57899999999999 37.0 37.0 40.0 33.0 40.0 64-65 35.409499999999994 37.0 37.0 37.0 33.0 40.0 66-67 34.999750000000006 37.0 33.0 37.0 27.0 40.0 68-69 34.864875 37.0 33.0 37.0 27.0 40.0 70-71 34.630375 37.0 33.0 37.0 30.0 40.0 72-73 34.355374999999995 37.0 33.0 37.0 27.0 38.5 74-75 33.979124999999996 37.0 33.0 37.0 27.0 37.0 76-77 31.3205 33.0 30.0 35.0 24.5 37.0 78-79 33.021125 37.0 33.0 37.0 27.0 37.0 80-81 33.272375 37.0 33.0 37.0 27.0 37.0 82-83 33.4365 37.0 33.0 37.0 27.0 37.0 84-85 33.375125 37.0 33.0 37.0 27.0 37.0 86-87 33.25425 37.0 33.0 37.0 27.0 37.0 88-89 32.98425 37.0 33.0 37.0 27.0 37.0 90-91 33.046375 37.0 33.0 37.0 27.0 37.0 92-93 32.827875 37.0 33.0 37.0 27.0 37.0 94-95 32.631125 37.0 33.0 37.0 27.0 37.0 96-97 32.342125 37.0 33.0 37.0 24.5 37.0 98-99 31.91225 37.0 33.0 37.0 22.0 37.0 100 27.967 33.0 27.0 33.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 5.0 11 4.0 12 4.0 13 4.0 14 2.0 15 8.0 16 7.0 17 9.0 18 9.0 19 6.0 20 12.0 21 7.0 22 15.0 23 16.0 24 14.0 25 24.0 26 21.0 27 33.0 28 43.0 29 47.0 30 54.0 31 80.0 32 118.0 33 141.0 34 162.0 35 261.0 36 566.0 37 1389.0 38 937.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.7548125633232 14.159067882472137 9.19452887537994 35.89159067882472 2 24.575 17.375 35.225 22.825 3 21.224999999999998 24.05 26.5 28.225 4 25.674999999999997 30.5 22.8 21.025 5 26.474999999999998 31.674999999999997 24.725 17.125 6 21.0 34.050000000000004 24.675 20.275000000000002 7 15.975 22.075 42.825 19.125 8 20.1 20.474999999999998 29.4 30.025000000000002 9 21.2 19.675 31.8 27.325 10-11 25.374999999999996 30.049999999999997 21.025 23.549999999999997 12-13 22.787499999999998 22.9875 27.8375 26.387500000000003 14-15 22.6375 25.825 26.125 25.412499999999998 16-17 23.075000000000003 24.625 26.6125 25.687500000000004 18-19 23.4125 26.437500000000004 25.7 24.45 20-21 23.25 25.387500000000003 26.2875 25.074999999999996 22-23 23.0 25.2375 26.575 25.1875 24-25 23.200000000000003 25.174999999999997 27.3375 24.2875 26-27 23.5875 26.375 26.724999999999998 23.3125 28-29 23.974999999999998 25.624999999999996 25.974999999999998 24.425 30-31 21.6 26.174999999999997 27.787499999999998 24.4375 32-33 22.625 25.9625 26.5125 24.9 34-35 22.400000000000002 24.5125 26.75 26.337500000000002 36-37 23.7625 26.4625 25.474999999999998 24.3 38-39 23.7125 25.7375 24.725 25.825 40-41 24.1875 25.724999999999998 25.837500000000002 24.25 42-43 23.375 25.974999999999998 26.525 24.125 44-45 23.65 25.825 26.400000000000002 24.125 46-47 23.575 26.9625 24.675 24.7875 48-49 24.0375 25.337500000000002 25.412499999999998 25.2125 50-51 24.1125 25.6125 25.112499999999997 25.162499999999998 52-53 24.65 24.4375 25.837500000000002 25.074999999999996 54-55 24.625 25.0125 25.674999999999997 24.6875 56-57 23.1875 25.124999999999996 25.837500000000002 25.85 58-59 22.7625 27.4125 24.975 24.85 60-61 22.787499999999998 26.4625 25.35 25.4 62-63 22.900000000000002 25.5375 27.1625 24.4 64-65 23.4125 25.8 25.95 24.837500000000002 66-67 22.3875 25.4375 26.0125 26.1625 68-69 23.4625 25.525 25.4 25.6125 70-71 23.4375 25.162499999999998 26.687499999999996 24.712500000000002 72-73 24.4375 24.4875 25.775 25.3 74-75 24.1625 25.3 25.2375 25.3 76-77 23.599999999999998 25.25 25.6 25.55 78-79 23.275000000000002 25.7 25.05 25.974999999999998 80-81 24.9 25.124999999999996 25.900000000000002 24.075 82-83 24.325 24.325 26.325 25.025 84-85 23.8125 26.0125 24.55 25.624999999999996 86-87 25.025 24.8 25.624999999999996 24.55 88-89 25.4625 25.35 25.174999999999997 24.0125 90-91 24.75 24.837500000000002 25.424999999999997 24.9875 92-93 23.4875 24.099999999999998 27.2625 25.15 94-95 23.1375 25.4 26.437500000000004 25.025 96-97 24.05 26.025 25.124999999999996 24.8 98-99 25.5 25.4625 25.1 23.9375 100 26.150000000000002 23.674999999999997 23.925 26.25 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 0.5 25 0.0 26 1.0 27 2.0 28 4.5 29 13.0 30 20.0 31 26.5 32 33.5 33 37.5 34 46.5 35 63.5 36 81.0 37 81.5 38 88.5 39 101.0 40 94.0 41 96.5 42 106.5 43 114.5 44 134.5 45 153.0 46 187.5 47 209.0 48 220.5 49 194.5 50 144.0 51 159.5 52 178.5 53 172.5 54 165.0 55 159.0 56 133.5 57 108.5 58 94.0 59 98.0 60 98.0 61 67.5 62 52.0 63 52.0 64 45.0 65 29.5 66 25.0 67 26.0 68 17.0 69 13.0 70 10.5 71 8.5 72 9.0 73 7.5 74 5.0 75 1.5 76 1.0 77 0.5 78 1.5 79 2.5 80 2.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.6 #Duplication Level Percentage of deduplicated Percentage of total 1 88.2046568627451 71.975 2 7.230392156862746 11.799999999999999 3 2.083333333333333 5.1 4 1.0723039215686274 3.5000000000000004 5 0.39828431372549017 1.625 6 0.39828431372549017 1.95 7 0.33700980392156865 1.925 8 0.12254901960784313 0.8 9 0.09191176470588235 0.675 >10 0.061274509803921566 0.65 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC 15 0.375 No Hit GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA 11 0.27499999999999997 No Hit GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT 9 0.22499999999999998 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 9 0.22499999999999998 No Hit CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA 9 0.22499999999999998 No Hit ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT 8 0.2 No Hit GCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCC 8 0.2 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 8 0.2 No Hit GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG 8 0.2 No Hit GTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGACAATC 7 0.17500000000000002 No Hit CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC 7 0.17500000000000002 No Hit GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC 7 0.17500000000000002 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 7 0.17500000000000002 No Hit ATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAG 7 0.17500000000000002 No Hit GGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAG 7 0.17500000000000002 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 7 0.17500000000000002 No Hit GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT 7 0.17500000000000002 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 7 0.17500000000000002 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 7 0.17500000000000002 No Hit GTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTCCGC 7 0.17500000000000002 No Hit GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT 6 0.15 No Hit GTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTG 6 0.15 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 6 0.15 No Hit CTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTT 6 0.15 No Hit GTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCG 6 0.15 No Hit CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG 6 0.15 No Hit GTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACA 6 0.15 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 6 0.15 No Hit GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA 6 0.15 No Hit CGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAG 6 0.15 No Hit CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT 6 0.15 No Hit CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAA 6 0.15 No Hit GCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGA 6 0.15 No Hit CTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGC 5 0.125 No Hit CAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGC 5 0.125 No Hit GTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGATC 5 0.125 No Hit CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT 5 0.125 No Hit GGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTG 5 0.125 No Hit AATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGATC 5 0.125 No Hit GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG 5 0.125 No Hit CAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAA 5 0.125 No Hit CCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCT 5 0.125 No Hit GTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG 5 0.125 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 5 0.125 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 5 0.125 No Hit GCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0125 0.0 0.0 0.0 0.0 48-49 0.037500000000000006 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1125 0.0 0.0 0.0 0.0 56-57 0.175 0.0 0.0 0.0 0.0 58-59 0.25 0.0 0.0 0.0 0.0 60-61 0.275 0.0 0.0 0.0 0.0 62-63 0.30000000000000004 0.0 0.0 0.0 0.0 64-65 0.35 0.0 0.0 0.0 0.0 66-67 0.4375 0.0 0.0 0.0 0.0 68-69 0.5125 0.0 0.0 0.0 0.0 70-71 0.675 0.0 0.0 0.0 0.0 72-73 0.7375 0.0 0.0 0.0 0.0 74-75 0.875 0.0 0.0 0.0 0.0 76-77 1.025 0.0 0.0 0.0 0.0 78-79 1.275 0.0 0.0 0.0 0.0 80-81 1.5125 0.0 0.0 0.0 0.0 82-83 1.725 0.0 0.0 0.0 0.0 84-85 2.1625 0.0 0.0 0.0 0.0 86-87 2.6500000000000004 0.0 0.0 0.0 0.0 88 3.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6127945 read2 length is 101 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6127945_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6565 33.0 33.0 33.0 33.0 33.0 2 31.71975 33.0 33.0 33.0 33.0 33.0 3 31.83325 33.0 33.0 33.0 33.0 33.0 4 35.315 37.0 37.0 37.0 33.0 37.0 5 35.29425 37.0 37.0 37.0 33.0 37.0 6 35.22475 37.0 37.0 37.0 33.0 37.0 7 35.2805 37.0 37.0 37.0 33.0 37.0 8 35.4285 37.0 37.0 37.0 33.0 37.0 9 35.62225 37.0 37.0 37.0 33.0 37.0 10-11 35.69375 37.0 37.0 37.0 37.0 37.0 12-13 35.690625 37.0 37.0 37.0 37.0 37.0 14-15 37.90625 40.0 37.0 40.0 37.0 40.0 16-17 37.91375 40.0 37.0 40.0 37.0 40.0 18-19 37.9 40.0 37.0 40.0 37.0 40.0 20-21 37.866875 40.0 37.0 40.0 37.0 40.0 22-23 37.14375 40.0 37.0 40.0 33.0 40.0 24-25 37.639375 40.0 37.0 40.0 35.0 40.0 26-27 37.70625 40.0 37.0 40.0 37.0 40.0 28-29 37.741875 40.0 37.0 40.0 37.0 40.0 30-31 37.6035 40.0 37.0 40.0 33.0 40.0 32-33 37.513999999999996 40.0 37.0 40.0 33.0 40.0 34-35 37.517375 40.0 37.0 40.0 35.0 40.0 36-37 37.411125 40.0 37.0 40.0 33.0 40.0 38-39 37.338375 40.0 37.0 40.0 33.0 40.0 40-41 37.221625 40.0 37.0 40.0 33.0 40.0 42-43 37.087374999999994 40.0 37.0 40.0 33.0 40.0 44-45 36.972 40.0 37.0 40.0 33.0 40.0 46-47 36.78775 40.0 37.0 40.0 33.0 40.0 48-49 36.591375 40.0 37.0 40.0 33.0 40.0 50-51 34.879374999999996 37.0 35.0 38.5 30.0 38.5 52-53 35.234750000000005 37.0 35.0 38.5 30.0 40.0 54-55 35.86475 37.0 37.0 40.0 33.0 40.0 56-57 35.877875 37.0 37.0 40.0 33.0 40.0 58-59 35.670625 37.0 37.0 40.0 33.0 40.0 60-61 35.29725 37.0 37.0 40.0 33.0 40.0 62-63 35.1555 37.0 37.0 37.0 33.0 40.0 64-65 35.03975 37.0 37.0 37.0 33.0 40.0 66-67 34.847 37.0 37.0 37.0 33.0 40.0 68-69 34.623875 37.0 35.0 37.0 33.0 40.0 70-71 34.526250000000005 37.0 33.0 37.0 33.0 40.0 72-73 34.31175 37.0 33.0 37.0 27.0 38.5 74-75 34.075125 37.0 33.0 37.0 27.0 37.0 76-77 33.811375 37.0 33.0 37.0 27.0 37.0 78-79 33.450125 37.0 33.0 37.0 27.0 37.0 80-81 33.441375 37.0 33.0 37.0 27.0 37.0 82-83 33.377 37.0 33.0 37.0 27.0 37.0 84-85 33.30575 37.0 33.0 37.0 27.0 37.0 86-87 33.263374999999996 37.0 33.0 37.0 27.0 37.0 88-89 33.1395 37.0 33.0 37.0 27.0 37.0 90-91 33.089875 37.0 33.0 37.0 27.0 37.0 92-93 33.0195 37.0 33.0 37.0 27.0 37.0 94-95 32.886375 37.0 33.0 37.0 27.0 37.0 96-97 32.582875 37.0 33.0 37.0 24.5 37.0 98-99 32.593625 37.0 33.0 37.0 27.0 37.0 100-101 31.137 35.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83.0 3 5.0 4 5.0 5 3.0 6 2.0 7 4.0 8 5.0 9 1.0 10 7.0 11 3.0 12 4.0 13 7.0 14 7.0 15 2.0 16 4.0 17 8.0 18 6.0 19 6.0 20 8.0 21 2.0 22 9.0 23 11.0 24 14.0 25 14.0 26 25.0 27 23.0 28 28.0 29 37.0 30 56.0 31 59.0 32 69.0 33 114.0 34 141.0 35 224.0 36 478.0 37 1579.0 38 947.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.074999999999996 18.35 9.925 32.65 2 28.275 24.575 27.55 19.6 3 22.475 26.424999999999997 28.000000000000004 23.1 4 26.3 34.2 18.775 20.724999999999998 5 26.474999999999998 35.15 19.05 19.325 6 22.425 36.725 20.5 20.349999999999998 7 20.724999999999998 18.55 36.95 23.775 8 22.650000000000002 22.825 24.349999999999998 30.175 9 24.525 22.8 26.150000000000002 26.525 10-11 25.662499999999998 28.9875 19.650000000000002 25.7 12-13 26.1125 22.875 24.075 26.937499999999996 14-15 24.4125 26.187500000000004 25.25 24.15 16-17 25.6125 25.4875 24.587500000000002 24.3125 18-19 25.7625 26.337500000000002 24.0 23.9 20-21 25.243810952738183 26.356589147286826 23.943485871467868 24.456114028507127 22-23 25.4875 26.487500000000004 24.6875 23.3375 24-25 25.1 26.1 23.95 24.85 26-27 26.337500000000002 26.325 23.799999999999997 23.5375 28-29 25.412499999999998 25.7375 24.7875 24.0625 30-31 25.3125 25.124999999999996 24.224999999999998 25.337500000000002 32-33 25.2875 26.2125 24.25 24.25 34-35 25.2 26.1 25.162499999999998 23.5375 36-37 25.2 25.0375 24.65 25.112499999999997 38-39 25.025 26.75 24.0 24.224999999999998 40-41 24.625 26.787499999999998 24.625 23.962500000000002 42-43 25.424999999999997 26.237500000000004 25.0125 23.325000000000003 44-45 25.75 25.15 25.0125 24.087500000000002 46-47 25.687500000000004 25.5375 25.0125 23.7625 48-49 25.5125 24.3875 25.124999999999996 24.975 50-51 24.175 25.7125 26.05 24.0625 52-53 25.05 25.6125 24.125 25.2125 54-55 25.4375 26.3 24.9 23.3625 56-57 24.725 26.375 25.0375 23.8625 58-59 25.2 25.275 24.9 24.625 60-61 24.975 26.275 25.837500000000002 22.912499999999998 62-63 24.8 26.5125 25.650000000000002 23.0375 64-65 25.05 26.174999999999997 24.337500000000002 24.4375 66-67 25.6 26.2125 24.7 23.4875 68-69 24.675 26.2875 25.5375 23.5 70-71 25.124999999999996 24.712500000000002 25.525 24.637500000000003 72-73 24.575 25.55 25.112499999999997 24.762500000000003 74-75 25.412499999999998 25.924999999999997 24.875 23.7875 76-77 24.7875 26.237500000000004 25.3125 23.6625 78-79 24.765595699462434 26.390798849856235 25.25315664458057 23.590448806100763 80-81 24.75 26.0625 25.337500000000002 23.849999999999998 82-83 25.36884221055264 25.30632658164541 24.956239059764943 24.36859214803701 84-85 25.196948855820935 25.497061398024258 25.30949105914718 23.996498687007627 86-87 25.0375 25.900000000000002 25.775 23.2875 88-89 26.1625 28.199999999999996 23.1375 22.5 90-91 25.5375 27.6375 24.3875 22.4375 92-93 25.7625 25.7625 25.025 23.45 94-95 26.487500000000004 27.037499999999998 24.762500000000003 21.712500000000002 96-97 26.6 26.4625 24.65 22.287499999999998 98-99 26.0 27.8125 23.4875 22.7 100-101 27.712500000000002 26.775 24.0 21.512500000000003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 2.0 21 1.5 22 0.5 23 1.0 24 1.0 25 1.0 26 1.0 27 1.0 28 4.0 29 7.0 30 11.5 31 20.0 32 27.0 33 31.0 34 36.5 35 48.0 36 54.5 37 65.5 38 93.5 39 116.0 40 120.0 41 101.0 42 93.5 43 111.0 44 139.5 45 153.0 46 165.5 47 188.0 48 193.5 49 193.5 50 172.5 51 154.5 52 164.5 53 161.5 54 154.5 55 167.5 56 157.5 57 124.0 58 120.5 59 115.0 60 95.5 61 81.0 62 63.0 63 49.5 64 43.5 65 37.5 66 28.5 67 27.5 68 19.0 69 12.0 70 11.5 71 9.5 72 10.5 73 8.5 74 4.0 75 2.5 76 2.0 77 3.5 78 4.5 79 3.0 80 1.0 81 0.5 82 1.5 83 1.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.025 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0125 80-81 0.0 82-83 0.025 84-85 0.0375 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.6 #Duplication Level Percentage of deduplicated Percentage of total 1 87.953995157385 72.65 2 7.717917675544794 12.75 3 2.1791767554479415 5.4 4 1.0290556900726393 3.4000000000000004 5 0.48426150121065376 2.0 6 0.3329297820823245 1.6500000000000001 7 0.1513317191283293 0.8750000000000001 8 0.03026634382566586 0.2 9 0.03026634382566586 0.22499999999999998 >10 0.09079903147699758 0.8500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 13 0.325 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 11 0.27499999999999997 No Hit GTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCG 10 0.25 No Hit GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT 9 0.22499999999999998 No Hit GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA 8 0.2 No Hit GCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGT 7 0.17500000000000002 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 7 0.17500000000000002 No Hit CGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAA 7 0.17500000000000002 No Hit GGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCAT 7 0.17500000000000002 No Hit CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG 7 0.17500000000000002 No Hit GCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGG 6 0.15 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 6 0.15 No Hit CCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTG 6 0.15 No Hit ATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACAT 6 0.15 No Hit CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG 6 0.15 No Hit GGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTACCCGGAT 6 0.15 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 6 0.15 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 6 0.15 No Hit GCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTG 6 0.15 No Hit GAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGC 6 0.15 No Hit AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC 6 0.15 No Hit GTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTG 5 0.125 No Hit CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC 5 0.125 No Hit CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCC 5 0.125 No Hit GTCGACTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTT 5 0.125 No Hit AGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGT 5 0.125 No Hit CCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCAC 5 0.125 No Hit GAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAA 5 0.125 No Hit CGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCA 5 0.125 No Hit CGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCG 5 0.125 No Hit GAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCG 5 0.125 No Hit GCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAG 5 0.125 No Hit CATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTG 5 0.125 No Hit CTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTT 5 0.125 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 5 0.125 No Hit GGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTG 5 0.125 No Hit GCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0125 0.0 0.0 0.0 0.0 48-49 0.037500000000000006 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1125 0.0 0.0 0.0 0.0 56-57 0.175 0.0 0.0 0.0 0.0 58-59 0.25 0.0 0.0 0.0 0.0 60-61 0.275 0.0 0.0 0.0 0.0 62-63 0.30000000000000004 0.0 0.0 0.0 0.0 64-65 0.36250000000000004 0.0 0.0 0.0 0.0 66-67 0.4625 0.0 0.0 0.0 0.0 68-69 0.5375 0.0 0.0 0.0 0.0 70-71 0.7 0.0 0.0 0.0 0.0 72-73 0.7625 0.0 0.0 0.0 0.0 74-75 0.9 0.0 0.0 0.0 0.0 76-77 1.0499999999999998 0.0 0.0 0.0 0.0 78-79 1.3 0.0 0.0 0.0 0.0 80-81 1.525 0.0 0.0 0.0 0.0 82-83 1.7625000000000002 0.0 0.0 0.0 0.0 84-85 2.1875 0.0 0.0 0.0 0.0 86-87 2.6875 0.0 0.0 0.0 0.0 88-89 3.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552921 spots for SRR6127945.sra Written 1552921 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra Read 1552911 spots for SRR6127945.sra Written 1552911 spots for SRR6127945.sra SRR ids: ['SRR6127945.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_nixtfmn5 SRR6127945.sra spots: 31058230 blocks: [[1, 1552911], [1552912, 3105822], [3105823, 4658733], [4658734, 6211644], [6211645, 7764555], [7764556, 9317466], [9317467, 10870377], [10870378, 12423288], [12423289, 13976199], [13976200, 15529110], [15529111, 17082021], [17082022, 18634932], [18634933, 20187843], [20187844, 21740754], [21740755, 23293665], [23293666, 24846576], [24846577, 26399487], [26399488, 27952398], [27952399, 29505309], [29505310, 31058230]] SRR6127945 file size 7409223 SRR6127945 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6127945 SRR6127945_1.fastq SRR6127945_2.fastq Input file: SRR6127945_1.fastq Paired file: SRR6127945_2.fastq trimmed: SRR6127945-trimmed-pair1.fastq, SRR6127945-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 04:49:26 2024 >> started Tue Dec 10 04:49:58 2024 >> done (31.049s) 31058230 read pairs processed; of these: 136592 ( 0.44%) short read pairs filtered out after trimming by size control 572345 ( 1.84%) empty read pairs filtered out after trimming by size control 30349293 (97.72%) read pairs available; of these: 6325163 (20.84%) trimmed read pairs available after processing 24024130 (79.16%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 16 0.00% 19 20 0.00% 20 40 0.00% 21 68 0.00% 22 102 0.00% 23 133 0.00% 24 229 0.00% 25 239 0.00% 26 291 0.00% 27 390 0.00% 28 493 0.00% 29 624 0.00% 30 645 0.00% 31 857 0.00% 32 993 0.00% 33 1181 0.00% 34 1371 0.00% 35 1463 0.00% 36 1750 0.01% 37 1868 0.01% 38 2257 0.01% 39 2487 0.01% 40 2698 0.01% 41 3095 0.01% 42 3367 0.01% 43 3807 0.01% 44 4077 0.01% 45 4621 0.02% 46 5033 0.02% 47 5417 0.02% 48 6007 0.02% 49 6529 0.02% 50 7188 0.02% 51 7831 0.03% 52 8629 0.03% 53 9392 0.03% 54 10140 0.03% 55 11099 0.04% 56 11995 0.04% 57 13264 0.04% 58 14987 0.05% 59 25569 0.08% 60 26560 0.09% 61 28106 0.09% 62 31390 0.10% 63 33070 0.11% 64 35576 0.12% 65 39110 0.13% 66 38706 0.13% 67 40511 0.13% 68 41863 0.14% 69 45987 0.15% 70 48602 0.16% 71 51754 0.17% 72 56035 0.18% 73 58428 0.19% 74 62007 0.20% 75 61209 0.20% 76 61641 0.20% 77 66786 0.22% 78 70513 0.23% 79 76439 0.25% 80 83045 0.27% 81 89643 0.30% 82 98674 0.33% 83 108584 0.36% 84 121665 0.40% 85 133358 0.44% 86 154484 0.51% 87 156246 0.51% 88 153663 0.51% 89 154005 0.51% 90 171161 0.56% 91 186611 0.61% 92 207957 0.69% 93 223252 0.74% 94 256929 0.85% 95 290609 0.96% 96 339096 1.12% 97 403370 1.33% 98 489291 1.61% 99 606683 2.00% 100 24764412 81.60% 30349293 reads passed initial QC criterion=sequence-density sequence-density=0.59 sequence-density-rank=1 fanout-score=1.91 fanout-score-rank=22 prefix-density=0.57 prefix-fanout=1.9 sequence=TTCAAATGTACA criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=33.59 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=1.3 sequence=TTTTTTTTCTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATTCCCGAGGCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCCCAGAGACGGGCGGAGGCTCTTAACCGTACGAG criterion=sequence-density sequence-density=0.59 sequence-density-rank=1 fanout-score=2.93 fanout-score-rank=14 prefix-density=0.68 prefix-fanout=2.6 sequence=GTGCAGTGCATCAGCTTCATCGCCTTCAAGCCACCGGGTTGTGAGGAGTCCGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=10.33 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=2.5 sequence=CGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCG SRR6127945 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 04:50:57 Started mapping on | Dec 10 04:50:57 Finished on | Dec 10 05:21:30 Mapping speed, Million of reads per hour | 59.61 Number of input reads | 30349293 Average input read length | 196 UNIQUE READS: Uniquely mapped reads number | 11867643 Uniquely mapped reads % | 39.10% Average mapped length | 195.35 Number of splices: Total | 4952945 Number of splices: Annotated (sjdb) | 4651114 Number of splices: GT/AG | 4887627 Number of splices: GC/AG | 50084 Number of splices: AT/AC | 1090 Number of splices: Non-canonical | 14144 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.10 Insertion rate per base | 0.03% Insertion average length | 2.04 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 970885 % of reads mapped to multiple loci | 3.20% Number of reads mapped to too many loci | 146574 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 54.98% % of reads unmapped: other | 2.24% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 17521231 17521231 17521231 N_multimapping 970885 970885 970885 N_noFeature 520838 11497510 625602 N_ambiguous 298118 1361 34134 UnstrandedReadsAssigned:11048687 PositiveStrandReadsAssigned:368772 NegativeStrandReadsAssigned:11207907 Dataset is classified negative stranded MeadianReadLen=100 20thPercentileLength=100 echo kmer=95 SRR6127945 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6127945-trimmed-pair1.fastq SRR6127945-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,349,293 reads, 11,356,105 reads pseudoaligned [quant] estimated average fragment length: 148.9 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,112 rounds 52973 SRR6127945.ke.tsv 35125 SRR6127945.se.tsv 88098 total ==> SRR6127945.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 788.189 0 0 PNS24247 1044 896.1 10.3638 1.27722 PNS24249 1928 1780.1 7.28281 0.451811 PNS24246 1044 896.1 10.3638 1.27722 PNS24248 1044 896.1 10.3638 1.27722 PNS24244 1471 1323.1 124.626 10.402 PNS24243 293 148.668 0 0 KQK14069 1603 1455.1 16684.1 1266.23 KQK14071 474 326.746 221.675 74.9217 ==> SRR6127945.se.tsv <== BRADI_1g14170v3 18131 BRADI_1g53295v3 48 BRADI_1g59795v3 16 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 62 BRADI_1g74790v3 46 BRADI_1g09890v3 0 BRADI_1g77505v3 102 BRADI_1g48960v3 0 SRR6127945 completed mapping pipeline successfully