Starting /dee2/code/volunteer_pipeline.sh SRR6257525 current disk space = 1544352407552 free memory = 1601532140 SRR6257525 SRAfilesize 4ade08beb2864c2c3e5b9d29874a2f9a SRR6257525.sra SRR6257525.sra file validated SRR6257525 is paired end SRR6257525 is conventional basespace SRR6257525 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6257525_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.8755 18.0 18.0 25.0 18.0 30.0 2 20.734 18.0 18.0 25.0 18.0 27.0 3 29.02025 28.0 27.0 32.0 27.0 32.0 4 28.65925 29.0 27.0 31.0 25.0 33.0 5 30.699 32.0 32.0 33.0 27.0 33.0 6 34.933 37.0 34.0 38.0 29.0 38.0 7 36.60575 38.0 37.0 38.0 34.0 38.0 8 37.2745 38.0 38.0 38.0 36.0 38.0 9 37.48575 38.0 38.0 38.0 37.0 38.0 10-14 37.3986 38.0 38.0 38.0 36.6 38.0 15-19 37.4847 38.0 38.0 38.0 37.4 38.0 20-24 37.4664 38.0 38.0 38.0 37.4 38.0 25-29 37.233999999999995 38.0 38.0 38.0 36.6 38.0 30-34 37.1018 38.0 38.0 38.0 36.2 38.0 35-39 37.3603 38.0 38.0 38.0 36.8 38.0 40-44 35.47935 37.8 34.8 38.0 30.0 38.0 45-49 37.07735 38.0 38.0 38.0 35.8 38.0 50-54 36.185449999999996 38.0 36.6 38.0 30.6 38.0 55-59 37.149150000000006 38.0 38.0 38.0 36.0 38.0 60-64 37.0141 38.0 38.0 38.0 35.6 38.0 65-69 35.187850000000005 37.8 34.0 38.0 29.0 38.0 70-74 37.019850000000005 38.0 38.0 38.0 35.2 38.0 75-79 36.52235 38.0 37.6 38.0 34.0 38.0 80-84 36.0238 38.0 37.2 38.0 31.8 38.0 85-89 36.452000000000005 38.0 38.0 38.0 34.0 38.0 90-94 34.2967 37.4 31.0 38.0 26.8 38.0 95-99 36.46275 38.0 38.0 38.0 34.0 38.0 100-104 36.26035 38.0 37.4 38.0 33.6 38.0 105-109 35.9326 38.0 36.6 38.0 32.6 38.0 110-114 35.51405 38.0 35.8 38.0 30.6 38.0 115-119 35.275549999999996 38.0 35.4 38.0 30.0 38.0 120-124 35.4879 38.0 35.6 38.0 31.4 38.0 125-129 35.0933 38.0 35.2 38.0 29.0 38.0 130-134 34.540800000000004 38.0 34.8 38.0 26.0 38.0 135-139 34.153800000000004 38.0 34.4 38.0 24.4 38.0 140-144 33.606 38.0 34.0 38.0 21.8 38.0 145-149 32.57885 38.0 33.4 38.0 15.4 38.0 150-151 28.181625 34.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 0.0 14 0.0 15 1.0 16 2.0 17 0.0 18 6.0 19 5.0 20 7.0 21 6.0 22 2.0 23 9.0 24 10.0 25 7.0 26 17.0 27 24.0 28 37.0 29 45.0 30 55.0 31 75.0 32 126.0 33 162.0 34 288.0 35 611.0 36 1469.0 37 1034.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.875164257555845 11.011826544021025 5.755584756898817 48.35742444152431 2 21.15 12.825000000000001 30.925000000000004 35.099999999999994 3 22.6 13.8 19.55 44.05 4 27.025 18.625 17.275 37.075 5 29.025000000000002 21.975 22.275 26.724999999999998 6 27.275 23.974999999999998 21.525 27.224999999999998 7 23.025000000000002 17.599999999999998 36.675000000000004 22.7 8 22.775000000000002 16.275000000000002 27.500000000000004 33.45 9 24.125 16.400000000000002 28.9 30.575000000000003 10-14 25.85 20.45 24.0 29.7 15-19 23.69 20.87 25.759999999999998 29.68 20-24 22.49 22.07 25.115 30.325000000000003 25-29 23.080000000000002 21.115000000000002 24.18 31.624999999999996 30-34 25.91 19.24 23.3 31.55 35-39 24.865000000000002 21.4 25.224999999999998 28.51 40-44 27.075 20.68 23.755000000000003 28.49 45-49 26.450000000000003 20.22 24.55 28.78 50-54 25.77 17.98 25.0 31.25 55-59 26.55 17.915 23.925 31.61 60-64 24.745 18.985 26.685 29.585 65-69 23.835 23.035 24.135 28.994999999999997 70-74 25.47 21.305 22.96 30.264999999999997 75-79 26.450000000000003 20.805 24.834999999999997 27.91 80-84 23.765 20.705000000000002 25.915 29.615000000000002 85-89 24.21 21.91 22.955000000000002 30.925000000000004 90-94 24.815 21.995 22.29 30.9 95-99 24.92 20.560000000000002 24.525 29.995 100-104 23.585 21.295 23.745 31.374999999999996 105-109 23.265 21.21 23.97 31.555 110-114 23.494999999999997 22.785 24.785 28.935 115-119 23.494999999999997 22.795 23.945 29.765000000000004 120-124 23.96 21.66 23.57 30.81 125-129 24.42 23.61 22.900000000000002 29.07 130-134 24.27 22.985 21.01 31.735000000000003 135-139 22.375 25.035 21.915000000000003 30.675 140-144 22.91 24.025 22.85 30.214999999999996 145-149 24.165 22.33 24.32 29.185 150-151 22.10276284535567 22.940367545943243 24.640580072509064 30.316289536192027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 1.0 25 1.5 26 1.0 27 0.5 28 0.5 29 1.5 30 2.0 31 5.0 32 5.0 33 6.0 34 8.5 35 7.0 36 10.0 37 15.0 38 17.0 39 22.0 40 21.0 41 14.5 42 19.5 43 25.5 44 30.0 45 37.0 46 52.0 47 84.0 48 116.0 49 133.5 50 187.5 51 228.0 52 211.5 53 225.5 54 239.5 55 217.5 56 225.0 57 228.0 58 199.0 59 179.5 60 174.0 61 198.5 62 215.0 63 190.0 64 134.0 65 68.5 66 29.5 67 25.0 68 23.5 69 10.0 70 9.5 71 12.0 72 9.0 73 7.5 74 7.0 75 13.0 76 32.5 77 37.0 78 19.5 79 6.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.875 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.24999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97233201581028 49.95 2 10.869565217391305 13.750000000000002 3 3.992094861660079 7.575 4 1.9367588932806323 4.9 5 1.1462450592885376 3.6249999999999996 6 0.7509881422924901 2.85 7 0.592885375494071 2.625 8 0.1976284584980237 1.0 9 0.2766798418972332 1.575 >10 1.225296442687747 10.775 >50 0.039525691699604744 1.375 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGC 55 1.375 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 29 0.7250000000000001 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 26 0.65 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 21 0.525 No Hit CCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT 20 0.5 No Hit CGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATT 19 0.475 No Hit CCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGA 17 0.42500000000000004 No Hit CACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTT 16 0.4 No Hit GCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTT 15 0.375 No Hit GTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGT 15 0.375 No Hit GCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 14 0.35000000000000003 No Hit GTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACAC 14 0.35000000000000003 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 13 0.325 No Hit GTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGAC 13 0.325 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 13 0.325 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 13 0.325 No Hit GTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA 13 0.325 No Hit GCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGG 12 0.3 No Hit CCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAG 12 0.3 No Hit GGCCGTTGCAGCGCAGTGCCCCGAGGGACACGCCTTGCGGCGCGCGGGTA 11 0.27499999999999997 No Hit CCTTGTTCCGCTCTCGCTCGCCGCTACTAACGGGGTCTCGGTTGATTTTC 11 0.27499999999999997 No Hit CGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACACGC 11 0.27499999999999997 No Hit CGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGG 11 0.27499999999999997 No Hit CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC 11 0.27499999999999997 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 11 0.27499999999999997 No Hit GTCACTTTCGTGTACCCATCGGACGGCAGCCCTTTCGGGGGTTCCTTAGG 10 0.25 No Hit CTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCC 10 0.25 No Hit CCCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGGCT 10 0.25 No Hit CACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCAC 10 0.25 No Hit CCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCG 10 0.25 No Hit CAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGT 10 0.25 No Hit ATCCGTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCAC 10 0.25 No Hit CAGTAGTCAAAGCAACTTCGTCACTTTCGTGTACCCATCGGACGGCAGCC 9 0.22499999999999998 No Hit CCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGT 9 0.22499999999999998 No Hit CAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGA 9 0.22499999999999998 No Hit GCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGTTG 9 0.22499999999999998 No Hit GCGCTATCTGCCGGGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGTG 9 0.22499999999999998 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 9 0.22499999999999998 No Hit GCCCCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGG 9 0.22499999999999998 No Hit CCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGT 8 0.2 No Hit CCGTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTC 8 0.2 No Hit GGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTG 8 0.2 No Hit CCAGGGTTGGCAGGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCC 8 0.2 No Hit GCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGCC 8 0.2 No Hit CTCCGGCTGAACCGATTCCAGGGTTGGCAGGCCGTTAAGCAGAAAAGATA 7 0.17500000000000002 No Hit CATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAA 7 0.17500000000000002 No Hit ACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACT 7 0.17500000000000002 No Hit CAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCT 7 0.17500000000000002 No Hit GGGGTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGC 7 0.17500000000000002 No Hit CACGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGC 7 0.17500000000000002 No Hit CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT 7 0.17500000000000002 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 7 0.17500000000000002 No Hit CTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGGCTCCAGCTATC 7 0.17500000000000002 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 7 0.17500000000000002 No Hit GCCGTTGCAGCGCAGTGCCCCGAGGGACACGCCTTGCGGCGCGCGGGTAC 7 0.17500000000000002 No Hit CCCTTGTTCCGCTCTCGCTCGCCGCTACTAACGGGGTCTCGGTTGATTTT 7 0.17500000000000002 No Hit CTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGG 7 0.17500000000000002 No Hit TGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGTT 7 0.17500000000000002 No Hit GTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGC 7 0.17500000000000002 No Hit ATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAA 6 0.15 No Hit CGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGA 6 0.15 No Hit CTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTC 6 0.15 No Hit CCAGTCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCG 6 0.15 No Hit CCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAAGCGGTAGGAGC 6 0.15 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 6 0.15 No Hit GTGCGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCC 6 0.15 No Hit CCGCCGGCGTCTCCGGACTTCCTAACGTCGCCGTCAACCGCCACATCCCG 6 0.15 No Hit CGTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCT 6 0.15 No Hit ATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAAC 6 0.15 No Hit GTAATGACTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTA 6 0.15 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 6 0.15 No Hit GCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGC 6 0.15 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 6 0.15 No Hit GGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGGCGTCTC 6 0.15 No Hit CGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGG 6 0.15 No Hit ATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTG 6 0.15 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 6 0.15 No Hit CCCGCCGGCGTCTCCGGACTTCCTAACGTCGCCGTCAACCGCCACATCCC 6 0.15 No Hit TACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTA 5 0.125 No Hit GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTA 5 0.125 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 5 0.125 No Hit CCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCT 5 0.125 No Hit TGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTAC 5 0.125 No Hit CCCCCATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTT 5 0.125 No Hit CGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTA 5 0.125 No Hit GGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCT 5 0.125 No Hit CTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCG 5 0.125 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 5 0.125 No Hit GGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCTCAATGCTTTG 5 0.125 No Hit CCTACCTCCGGCTGAACCGATTCCAGGGTTGGCAGGCCGTTAAGCAGAAA 5 0.125 No Hit CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAG 5 0.125 No Hit GGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTC 5 0.125 No Hit CCCCAGTCGAAGACCCCACCGTGGTATGCGCCAATAAGACCACCAAAGGC 5 0.125 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 5 0.125 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 5 0.125 No Hit CCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCGTGCTCCA 5 0.125 No Hit GGGTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGCA 5 0.125 No Hit CTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCACGCGGCA 5 0.125 No Hit GTTCAGTAGTCAAAGCAACTTCGTCACTTTCGTGTACCCATCGGACGGCA 5 0.125 No Hit CGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCT 5 0.125 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 5 0.125 No Hit GTCCCGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCG 5 0.125 No Hit GTTGGCAGGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCG 5 0.125 No Hit CCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCA 5 0.125 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 5 0.125 No Hit CGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTG 5 0.125 No Hit GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0125 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.037500000000000006 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.0625 0.0 0.0 0.0 0.0 54-55 0.0875 0.0 0.0 0.0 0.0 56-57 0.1125 0.0 0.0 0.0 0.0 58-59 0.1375 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.225 0.0 0.0 0.0 0.0 68-69 0.3375 0.0 0.0 0.0 0.0 70-71 0.4125 0.0 0.0 0.0 0.0 72-73 0.48750000000000004 0.0 0.0 0.0 0.0 74-75 0.6375 0.0 0.0 0.0 0.0 76-77 0.85 0.0 0.0 0.0 0.0 78-79 0.95 0.0 0.0 0.0 0.0 80-81 1.0125 0.0 0.0 0.0 0.0 82-83 1.175 0.0 0.0 0.0 0.0 84-85 1.5125 0.0 0.0 0.0 0.0 86-87 1.8125 0.0 0.0 0.0 0.0 88-89 2.0125 0.0 0.0 0.0 0.0 90-91 2.2750000000000004 0.0 0.0 0.0 0.0 92-93 2.7 0.0 0.0 0.0 0.0 94-95 3.2375 0.0 0.0 0.0 0.0 96-97 3.6500000000000004 0.0 0.0 0.0 0.0 98-99 4.1375 0.0 0.0 0.0 0.0 100-101 4.7875 0.0 0.0 0.0 0.0 102-103 5.4625 0.0 0.0 0.0 0.0 104-105 6.3 0.0 0.0 0.0 0.0 106-107 7.025 0.0 0.0 0.0 0.0 108-109 7.725 0.0 0.0 0.0 0.0 110-111 8.65 0.0 0.0 0.0 0.0 112-113 9.65 0.0 0.0 0.0 0.0 114-115 10.6625 0.0 0.0 0.0 0.0 116-117 12.1625 0.0 0.0 0.0 0.0 118-119 13.475 0.0 0.0 0.0 0.0 120-121 14.524999999999999 0.0 0.0 0.0 0.0 122-123 15.7875 0.0 0.0 0.0 0.0 124-125 17.4625 0.0 0.0 0.0 0.0 126-127 18.6625 0.0 0.0 0.0 0.0 128-129 20.0625 0.0 0.0 0.0 0.0 130-131 21.450000000000003 0.0 0.0 0.0 0.0 132-133 22.8875 0.0 0.0 0.0 0.0 134-135 24.25 0.0 0.0 0.0 0.0 136-137 25.4 0.0 0.0 0.0 0.0 138-139 26.262500000000003 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGTCA 10 0.005853838 152.57895 1 GGGTCAT 10 0.0068378756 144.95 2 GTCATAT 10 0.0068378756 144.95 4 ATCTAGT 10 0.0068378756 144.95 9 GGTCATA 10 0.0068378756 144.95 3 TATCTAG 10 0.0068378756 144.95 8 ATATCTA 10 0.0068378756 144.95 7 CATATCT 10 0.0068378756 144.95 6 TCATATC 10 0.0068378756 144.95 5 AGTCACG 35 0.0033169514 62.121426 145 TACCTCC 40 2.965456E-4 21.7425 20-24 CTACCTC 40 0.0076702754 18.11875 20-24 >>END_MODULE SRR6257525 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6257525_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8295 33.0 33.0 34.0 32.0 34.0 2 32.88 34.0 33.0 34.0 32.0 34.0 3 32.916 34.0 33.0 34.0 32.0 34.0 4 32.98825 34.0 33.0 34.0 32.0 34.0 5 32.96375 34.0 33.0 34.0 32.0 34.0 6 37.199 38.0 38.0 38.0 37.0 38.0 7 33.54125 38.0 33.0 38.0 16.0 38.0 8 35.9945 38.0 36.0 38.0 30.0 38.0 9 35.923 38.0 37.0 38.0 31.0 38.0 10-14 36.93599999999999 38.0 38.0 38.0 35.8 38.0 15-19 36.8803 38.0 38.0 38.0 35.4 38.0 20-24 37.183299999999996 38.0 38.0 38.0 36.8 38.0 25-29 37.23765 38.0 38.0 38.0 37.0 38.0 30-34 37.2245 38.0 38.0 38.0 37.0 38.0 35-39 37.1361 38.0 38.0 38.0 36.8 38.0 40-44 35.9815 38.0 36.0 38.0 30.8 38.0 45-49 36.77165 38.0 37.8 38.0 35.0 38.0 50-54 36.69975 38.0 38.0 38.0 35.2 38.0 55-59 36.650749999999995 38.0 38.0 38.0 34.4 38.0 60-64 36.8667 38.0 38.0 38.0 35.6 38.0 65-69 36.75790000000001 38.0 38.0 38.0 35.0 38.0 70-74 36.57715 38.0 38.0 38.0 34.2 38.0 75-79 35.03295 37.8 34.6 38.0 28.4 38.0 80-84 35.96535 38.0 37.0 38.0 30.2 38.0 85-89 35.279900000000005 38.0 35.8 38.0 28.2 38.0 90-94 35.867149999999995 38.0 37.4 38.0 32.2 38.0 95-99 32.7444 35.4 29.8 38.0 24.8 38.0 100-104 35.85095 38.0 36.4 38.0 32.4 38.0 105-109 36.1229 38.0 38.0 38.0 33.2 38.0 110-114 35.802350000000004 38.0 36.6 38.0 32.4 38.0 115-119 34.339749999999995 37.6 33.2 38.0 27.2 38.0 120-124 34.28635 37.6 34.0 38.0 26.6 38.0 125-129 29.66705 33.0 24.0 37.0 16.4 38.0 130-134 28.49975 31.0 22.0 37.4 16.2 38.0 135-139 33.00665 37.6 32.8 38.0 21.8 38.0 140-144 28.45735 31.8 24.4 36.6 10.2 38.0 145-149 28.3876 33.6 25.6 37.4 2.0 38.0 150-151 24.777250000000002 31.5 14.5 36.5 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 3.0 4 3.0 5 0.0 6 0.0 7 2.0 8 1.0 9 0.0 10 0.0 11 0.0 12 3.0 13 1.0 14 4.0 15 0.0 16 2.0 17 2.0 18 4.0 19 8.0 20 12.0 21 8.0 22 9.0 23 13.0 24 23.0 25 31.0 26 26.0 27 34.0 28 46.0 29 63.0 30 72.0 31 116.0 32 167.0 33 208.0 34 426.0 35 796.0 36 1411.0 37 501.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 45.125 12.7 8.200000000000001 33.975 2 36.625 15.4 20.65 27.325 3 32.300000000000004 21.175 21.325 25.2 4 31.974999999999998 31.674999999999997 16.075 20.275000000000002 5 32.25 28.4 16.6 22.75 6 30.55 33.650000000000006 15.775 20.025000000000002 7 30.025000000000002 18.475 31.8 19.7 8 31.374999999999996 18.9 23.95 25.775 9 29.625 21.25 24.325 24.8 10-14 29.659999999999997 24.98 22.455 22.905 15-19 30.220000000000002 23.875 25.03 20.875 20-24 31.005 23.380000000000003 22.795 22.82 25-29 29.641482074103703 24.55122756137807 21.69108455422771 24.116205810290513 30-34 32.789918487773164 22.893434015102265 21.008151222683402 23.308496274441165 35-39 34.391878375675134 24.80996199239848 20.33906781356271 20.459091818363675 40-44 34.333433343334335 23.857385738573857 20.8970897089709 20.912091209120913 45-49 31.66 25.045 20.32 22.975 50-54 30.395 25.019999999999996 19.43 25.155 55-59 29.525000000000002 25.295 22.66 22.52 60-64 31.395 23.505000000000003 21.925 23.175 65-69 32.41 22.830000000000002 19.625 25.135 70-74 32.365 25.924999999999997 17.27 24.44 75-79 31.525 25.595000000000002 19.42 23.46 80-84 32.67663383169159 23.711185559277965 18.855942797139857 24.756237811890593 85-89 30.581529076453823 23.691184559227963 19.82099104955248 25.906295314765735 90-94 32.074999999999996 25.019999999999996 19.41 23.494999999999997 95-99 30.055 24.490000000000002 21.48 23.974999999999998 100-104 29.759999999999998 24.44 22.085 23.715 105-109 29.21 22.115000000000002 23.275000000000002 25.4 110-114 29.160000000000004 25.835 19.895 25.11 115-119 30.880000000000003 23.265 20.285 25.569999999999997 120-124 30.44804480448045 24.597459745974597 20.77707770777078 24.17741774177418 125-129 29.921496074803738 25.131256562828142 21.21606080304015 23.731186559327966 130-134 31.89 25.580000000000002 19.830000000000002 22.7 135-139 32.54162708135407 23.226161308065404 20.426021301065052 23.806190309515475 140-144 32.455 23.669999999999998 20.51 23.365 145-149 33.01 24.755 20.89 21.345 150-151 31.612499999999997 26.825 23.599999999999998 17.962500000000002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 0.5 25 0.5 26 1.0 27 0.5 28 1.0 29 1.5 30 3.0 31 4.5 32 4.0 33 5.0 34 6.0 35 9.0 36 14.5 37 13.0 38 9.0 39 14.0 40 15.5 41 17.0 42 23.0 43 26.0 44 35.0 45 51.5 46 80.5 47 129.5 48 175.5 49 205.5 50 202.5 51 211.5 52 219.5 53 205.0 54 237.5 55 278.5 56 236.5 57 172.0 58 155.0 59 135.0 60 124.5 61 130.5 62 131.5 63 123.0 64 82.5 65 49.5 66 49.0 67 56.5 68 55.0 69 37.5 70 34.5 71 32.0 72 17.5 73 14.0 74 20.5 75 31.5 76 50.0 77 39.5 78 12.5 79 4.0 80 1.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.005 30-34 0.015 35-39 0.02 40-44 0.01 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.005 85-89 0.005 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.01 125-129 0.005 130-134 0.0 135-139 0.005 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.39999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 78.41312056737588 44.224999999999994 2 10.99290780141844 12.4 3 3.50177304964539 5.925 4 1.950354609929078 4.3999999999999995 5 1.4184397163120568 4.0 6 0.6648936170212766 2.25 7 0.4875886524822695 1.925 8 0.5319148936170213 2.4 9 0.044326241134751775 0.22499999999999998 >10 1.9060283687943262 18.575 >50 0.08865248226950355 3.675 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGAC 84 2.1 No Hit CCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATC 63 1.575 No Hit GCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGC 42 1.05 No Hit GGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCA 38 0.95 No Hit GCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCC 33 0.8250000000000001 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 29 0.7250000000000001 No Hit CGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGC 26 0.65 No Hit CGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCA 25 0.625 No Hit GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC 22 0.5499999999999999 No Hit GCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAA 22 0.5499999999999999 No Hit CCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAAT 20 0.5 No Hit GTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCGCCTCG 20 0.5 No Hit AGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGA 20 0.5 No Hit CTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCA 20 0.5 No Hit GGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAG 19 0.475 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 19 0.475 No Hit GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGT 18 0.44999999999999996 No Hit GCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATA 18 0.44999999999999996 No Hit CATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCT 18 0.44999999999999996 No Hit CGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATG 18 0.44999999999999996 No Hit CATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGG 17 0.42500000000000004 No Hit GGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTA 17 0.42500000000000004 No Hit GGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAA 16 0.4 No Hit AGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAA 16 0.4 No Hit GGGAGCGTTCCGCCTTAGAGGGAAGCAACCGCGAAAGCGGGGGTCGACGA 15 0.375 No Hit CGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACG 15 0.375 No Hit GCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTG 14 0.35000000000000003 No Hit CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAAC 14 0.35000000000000003 No Hit GGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAAC 13 0.325 No Hit CTGTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCCCCC 13 0.325 No Hit AGGGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGC 12 0.3 No Hit CCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATT 12 0.3 No Hit GGACTGTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCC 12 0.3 No Hit GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT 12 0.3 No Hit GGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAA 12 0.3 No Hit CAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTG 11 0.27499999999999997 No Hit GGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCAT 11 0.27499999999999997 No Hit CTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTCCGGTGAGCCGCGCC 11 0.27499999999999997 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 11 0.27499999999999997 No Hit GACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGG 11 0.27499999999999997 No Hit AAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCG 11 0.27499999999999997 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 10 0.25 No Hit GACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCAT 10 0.25 No Hit CCCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGA 10 0.25 No Hit GTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCCCCCGG 10 0.25 No Hit CGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATC 9 0.22499999999999998 No Hit CCGCTCGGCTCGGGGCGTGGACTGTTGTCGGCCGTGCTGGCGGCCCAAGC 8 0.2 No Hit GCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCGC 8 0.2 No Hit CTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTCCGGTGAGCC 8 0.2 No Hit GGCGTGGACTGTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAG 8 0.2 No Hit GGGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCC 8 0.2 No Hit GAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGG 8 0.2 No Hit GAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGG 8 0.2 No Hit TTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTC 8 0.2 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 8 0.2 No Hit CGTGCCTCCGGCATCGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGC 8 0.2 No Hit GCGGGACGTTCTGTCAATCGGGGAAGGTTTTTGGTGACAAGACCTGGAGA 8 0.2 No Hit CCGACTGATGTTGAAGAATCAGCGGATGAGTTGTGGTTAGGGGTGAAATG 8 0.2 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 7 0.17500000000000002 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 7 0.17500000000000002 No Hit CGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTG 7 0.17500000000000002 No Hit ATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACGTTA 7 0.17500000000000002 No Hit GGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCG 7 0.17500000000000002 No Hit GTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTCCCCTGGAAAG 7 0.17500000000000002 No Hit ACCGACTGATGTTGAAGAATCAGCGGATGAGTTGTGGTTAGGGGTGAAAT 7 0.17500000000000002 No Hit CCGACATGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATC 7 0.17500000000000002 No Hit GCTAGGCGAAGCGGTTGAGTGCCGCACCCTAGATGGCTAAAGTCCAGTAG 7 0.17500000000000002 No Hit CAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATC 7 0.17500000000000002 No Hit CGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGG 7 0.17500000000000002 No Hit CGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCC 6 0.15 No Hit CAAACTTTAAATAGGTAGGACGGCGCGGCTGCTCCGGTGAGCCGCGCCAT 6 0.15 No Hit GGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGT 6 0.15 No Hit GTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACG 6 0.15 No Hit CCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGG 6 0.15 No Hit GGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGAT 6 0.15 No Hit GCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACGGAACCCACCGGA 6 0.15 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 6 0.15 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 6 0.15 No Hit CTCCGGCATCGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCATCG 6 0.15 No Hit GCCGACGAGCCGCGTCCGGCCGCCTCGAAGCTCCCTTCCCCACGGGCGGC 6 0.15 No Hit CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA 6 0.15 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 6 0.15 No Hit TGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACGGA 6 0.15 No Hit GTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGT 6 0.15 No Hit GGACTGGTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCC 5 0.125 No Hit GGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGAC 5 0.125 No Hit AGCGGGACGTTCTGTCAATCGGGGAAGGTTTTTGGTGACAAGACCTGGAG 5 0.125 No Hit GCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTG 5 0.125 No Hit CGGGACGTTCTGTCAATCGGGGAAGGTTTTTGGTGACAAGACCTGGAGAT 5 0.125 No Hit ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAA 5 0.125 No Hit CCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTCCGGTGAGC 5 0.125 No Hit GCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAA 5 0.125 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 5 0.125 No Hit AATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACGTT 5 0.125 No Hit GGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACG 5 0.125 No Hit TGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCA 5 0.125 No Hit TGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCT 5 0.125 No Hit GCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGG 5 0.125 No Hit GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAG 5 0.125 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTTGTTGTGTAGATCT 5 0.125 Illumina Single End PCR Primer 1 (96% over 32bp) GTCGGCCAGTGAGACGATGGGGGATAAGCTTCATCGTCGAGAGGGAAACA 5 0.125 No Hit GCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTT 5 0.125 No Hit CCGAAATGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGAC 5 0.125 No Hit CACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGA 5 0.125 No Hit GCGAAAGCGAGTCTTCATAGGGCGATTGTCACTGCTTATGGACCCGAACC 5 0.125 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 5 0.125 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 5 0.125 No Hit CTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGC 5 0.125 No Hit CCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTC 5 0.125 No Hit CATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAACAA 5 0.125 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 5 0.125 No Hit GTTTTTGGTGACAAGACCTGGAGATATCAGAAGTGAGAATGCTGACATGA 5 0.125 No Hit CCGAAACCGATCGATCTAGCCATGAGCAGGTTGAAGAGAGCTCTAACAGG 5 0.125 No Hit ACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGG 5 0.125 No Hit AGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAG 5 0.125 No Hit CCCCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0125 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.037500000000000006 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.0625 0.0 0.0 0.0 0.0 54-55 0.0875 0.0 0.0 0.0 0.0 56-57 0.1125 0.0 0.0 0.0 0.0 58-59 0.1375 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.225 0.0 0.0 0.0 0.0 68-69 0.325 0.0 0.0 0.0 0.0 70-71 0.4 0.0 0.0 0.0 0.0 72-73 0.48750000000000004 0.0 0.0 0.0 0.0 74-75 0.625 0.0 0.0 0.0 0.0 76-77 0.775 0.0 0.0 0.0 0.0 78-79 0.875 0.0 0.0 0.0 0.0 80-81 0.9624999999999999 0.0 0.0 0.0 0.0 82-83 1.1 0.0 0.0 0.0 0.0 84-85 1.525 0.0 0.0 0.0 0.0 86-87 1.8125 0.0 0.0 0.0 0.0 88-89 1.9625 0.0 0.0 0.0 0.0 90-91 2.1875 0.0 0.0 0.0 0.0 92-93 2.525 0.0 0.0 0.0 0.0 94-95 2.925 0.0 0.0 0.0 0.0 96-97 3.175 0.0 0.0 0.0 0.0 98-99 3.6375 0.0 0.0 0.0 0.0 100-101 4.300000000000001 0.0 0.0 0.0 0.0 102-103 5.0125 0.0 0.0 0.0 0.0 104-105 5.862500000000001 0.0 0.0 0.0 0.0 106-107 6.6375 0.0 0.0 0.0 0.0 108-109 7.324999999999999 0.0 0.0 0.0 0.0 110-111 8.1875 0.0 0.0 0.0 0.0 112-113 9.1 0.0 0.0 0.0 0.0 114-115 9.9 0.0 0.0 0.0 0.0 116-117 10.9 0.0 0.0 0.0 0.0 118-119 11.8125 0.0 0.0 0.0 0.0 120-121 12.287500000000001 0.0 0.0 0.0 0.0 122-123 12.9875 0.0 0.0 0.0 0.0 124-125 13.9375 0.0 0.0 0.0 0.0 126-127 14.6 0.0 0.0 0.0 0.0 128-129 15.587499999999999 0.0 0.0 0.0 0.0 130-131 16.612499999999997 0.0 0.0 0.0 0.0 132-133 17.6625 0.0 0.0 0.0 0.0 134-135 18.65 0.0 0.0 0.0 0.0 136-137 19.4 0.0 0.0 0.0 0.0 138-139 20.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTCA 15 1.1411342E-4 145.0 2 ACTCATA 15 1.1411342E-4 145.0 4 GCAAATT 15 1.1411342E-4 145.0 145 GCACGTG 10 0.006830828 145.0 4 CGTACTC 15 1.1411342E-4 145.0 1 AAAGGGA 15 1.1411342E-4 145.0 4 TACTCAT 15 1.1411342E-4 145.0 3 GAAAGGG 15 1.1411342E-4 145.0 3 CATCATT 20 3.5877043E-4 108.75 2 ATAACCG 20 3.5877043E-4 108.75 8 AGGGAAT 20 3.5877043E-4 108.75 6 TAACCGC 20 3.5877043E-4 108.75 9 ATCATTC 20 3.5877043E-4 108.75 3 GGAGGTA 20 3.5877043E-4 108.75 145 TCATTCA 20 3.5877043E-4 108.75 4 CTCATAA 20 3.5877043E-4 108.75 5 GCATCAT 20 3.5877043E-4 108.75 1 TCATAAC 20 3.5877043E-4 108.75 6 CATAACC 20 3.5877043E-4 108.75 7 CATTCAA 25 8.7132835E-4 87.0 5 >>END_MODULE Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769317 spots for SRR6257525.sra Written 769317 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra Read 769305 spots for SRR6257525.sra Written 769305 spots for SRR6257525.sra SRR ids: ['SRR6257525.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_e5nlssr1 SRR6257525.sra spots: 15386112 blocks: [[1, 769305], [769306, 1538610], [1538611, 2307915], [2307916, 3077220], [3077221, 3846525], [3846526, 4615830], [4615831, 5385135], [5385136, 6154440], [6154441, 6923745], [6923746, 7693050], [7693051, 8462355], [8462356, 9231660], [9231661, 10000965], [10000966, 10770270], [10770271, 11539575], [11539576, 12308880], [12308881, 13078185], [13078186, 13847490], [13847491, 14616795], [14616796, 15386112]] SRR6257525 file size 5192148 SRR6257525 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6257525 SRR6257525_1.fastq SRR6257525_2.fastq Input file: SRR6257525_1.fastq Paired file: SRR6257525_2.fastq trimmed: SRR6257525-trimmed-pair1.fastq, SRR6257525-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 09:02:43 2024 >> started Sat Dec 7 09:03:11 2024 >> done (28.151s) 15386112 read pairs processed; of these: 8600 ( 0.06%) short read pairs filtered out after trimming by size control 37621 ( 0.24%) empty read pairs filtered out after trimming by size control 15339891 (99.70%) read pairs available; of these: 10030707 (65.39%) trimmed read pairs available after processing 5309184 (34.61%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 4 0.00% 20 11 0.00% 21 16 0.00% 22 31 0.00% 23 41 0.00% 24 38 0.00% 25 52 0.00% 26 109 0.00% 27 128 0.00% 28 147 0.00% 29 139 0.00% 30 157 0.00% 31 153 0.00% 32 151 0.00% 33 139 0.00% 34 202 0.00% 35 217 0.00% 36 235 0.00% 37 245 0.00% 38 291 0.00% 39 385 0.00% 40 405 0.00% 41 494 0.00% 42 459 0.00% 43 430 0.00% 44 446 0.00% 45 476 0.00% 46 494 0.00% 47 610 0.00% 48 770 0.01% 49 904 0.01% 50 1087 0.01% 51 1069 0.01% 52 1122 0.01% 53 1152 0.01% 54 1215 0.01% 55 1205 0.01% 56 1355 0.01% 57 1489 0.01% 58 1666 0.01% 59 1761 0.01% 60 1964 0.01% 61 2727 0.02% 62 2806 0.02% 63 3724 0.02% 64 3695 0.02% 65 3805 0.02% 66 4536 0.03% 67 5756 0.04% 68 6455 0.04% 69 6639 0.04% 70 7504 0.05% 71 7894 0.05% 72 9023 0.06% 73 9306 0.06% 74 9532 0.06% 75 9927 0.06% 76 10761 0.07% 77 11055 0.07% 78 11832 0.08% 79 12858 0.08% 80 14328 0.09% 81 15705 0.10% 82 20478 0.13% 83 22070 0.14% 84 21912 0.14% 85 21160 0.14% 86 20391 0.13% 87 22451 0.15% 88 20479 0.13% 89 22479 0.15% 90 22090 0.14% 91 25994 0.17% 92 31678 0.21% 93 31785 0.21% 94 36903 0.24% 95 38901 0.25% 96 44625 0.29% 97 45816 0.30% 98 58988 0.38% 99 53773 0.35% 100 57205 0.37% 101 56710 0.37% 102 59392 0.39% 103 61879 0.40% 104 64048 0.42% 105 59249 0.39% 106 62530 0.41% 107 67187 0.44% 108 59012 0.38% 109 64148 0.42% 110 67135 0.44% 111 71942 0.47% 112 95879 0.63% 113 78924 0.51% 114 86679 0.57% 115 92094 0.60% 116 100814 0.66% 117 91505 0.60% 118 99961 0.65% 119 103322 0.67% 120 107289 0.70% 121 100642 0.66% 122 123863 0.81% 123 113456 0.74% 124 115913 0.76% 125 113658 0.74% 126 122156 0.80% 127 132115 0.86% 128 102509 0.67% 129 120417 0.78% 130 119193 0.78% 131 117995 0.77% 132 117182 0.76% 133 121818 0.79% 134 103506 0.67% 135 105561 0.69% 136 105398 0.69% 137 115885 0.76% 138 113444 0.74% 139 119863 0.78% 140 126190 0.82% 141 144761 0.94% 142 152410 0.99% 143 167515 1.09% 144 183950 1.20% 145 196835 1.28% 146 235681 1.54% 147 288720 1.88% 148 398320 2.60% 149 735245 4.79% 150 2854287 18.61% 151 5309184 34.61% 15339891 reads passed initial QC criterion=sequence-density sequence-density=5.76 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=27 prefix-density=5.80 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.45 sequence-density-rank=18 fanout-score=28.46 fanout-score-rank=1 prefix-density=12.88 prefix-fanout=1.0 sequence=CGGTGTGTACAGGGCCCGGGTACATATTCACCGCGGCATGCTGATCCGCGATTACTAG criterion=sequence-density sequence-density=4.32 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=29 prefix-density=4.39 prefix-fanout=2.0 sequence=CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGGCGATGC criterion=fanout-score sequence-density=0.37 sequence-density-rank=18 fanout-score=22.39 fanout-score-rank=1 prefix-density=3.77 prefix-fanout=2.2 sequence=GACCGATAGCGTACAAGTACCGTGAG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGGCGATGC -o SRR6257525 SRR6257525_1.fastq SRR6257525_2.fastq Input file: SRR6257525_1.fastq Paired file: SRR6257525_2.fastq trimmed: SRR6257525-trimmed-pair1.fastq, SRR6257525-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 09:04:16 2024 >> started Sat Dec 7 09:04:31 2024 >> done (14.513s) 10226594 read pairs processed; of these: 306 ( 0.00%) short read pairs filtered out after trimming by size control 448 ( 0.00%) empty read pairs filtered out after trimming by size control 10225840 (99.99%) read pairs available; of these: 7025 ( 0.07%) trimmed read pairs available after processing 10218815 (99.93%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 3 0.00% 20 7 0.00% 21 9 0.00% 22 20 0.00% 23 23 0.00% 24 30 0.00% 25 32 0.00% 26 70 0.00% 27 91 0.00% 28 92 0.00% 29 95 0.00% 30 90 0.00% 31 96 0.00% 32 104 0.00% 33 88 0.00% 34 134 0.00% 35 150 0.00% 36 149 0.00% 37 173 0.00% 38 178 0.00% 39 249 0.00% 40 272 0.00% 41 311 0.00% 42 301 0.00% 43 283 0.00% 44 296 0.00% 45 304 0.00% 46 315 0.00% 47 413 0.00% 48 513 0.01% 49 630 0.01% 50 703 0.01% 51 696 0.01% 52 739 0.01% 53 782 0.01% 54 836 0.01% 55 802 0.01% 56 879 0.01% 57 967 0.01% 58 1100 0.01% 59 1169 0.01% 60 1300 0.01% 61 1807 0.02% 62 1854 0.02% 63 2496 0.02% 64 2435 0.02% 65 2541 0.02% 66 3000 0.03% 67 3809 0.04% 68 4298 0.04% 69 4410 0.04% 70 5022 0.05% 71 5295 0.05% 72 6033 0.06% 73 6120 0.06% 74 6364 0.06% 75 6521 0.06% 76 7201 0.07% 77 7367 0.07% 78 7850 0.08% 79 8581 0.08% 80 9639 0.09% 81 10504 0.10% 82 13589 0.13% 83 14789 0.14% 84 14509 0.14% 85 14179 0.14% 86 13518 0.13% 87 15019 0.15% 88 13674 0.13% 89 14871 0.15% 90 14627 0.14% 91 17222 0.17% 92 21142 0.21% 93 21311 0.21% 94 24648 0.24% 95 25916 0.25% 96 29673 0.29% 97 30497 0.30% 98 39421 0.39% 99 35852 0.35% 100 38189 0.37% 101 37768 0.37% 102 39642 0.39% 103 41260 0.40% 104 42684 0.42% 105 39659 0.39% 106 41673 0.41% 107 44724 0.44% 108 39595 0.39% 109 42822 0.42% 110 44724 0.44% 111 47907 0.47% 112 63697 0.62% 113 52738 0.52% 114 57919 0.57% 115 61322 0.60% 116 67242 0.66% 117 61133 0.60% 118 66777 0.65% 119 68788 0.67% 120 71773 0.70% 121 67087 0.66% 122 82449 0.81% 123 75668 0.74% 124 77448 0.76% 125 75841 0.74% 126 81175 0.79% 127 87811 0.86% 128 68548 0.67% 129 80652 0.79% 130 79606 0.78% 131 78602 0.77% 132 78500 0.77% 133 81295 0.79% 134 68851 0.67% 135 70338 0.69% 136 70109 0.69% 137 77382 0.76% 138 75851 0.74% 139 80158 0.78% 140 83958 0.82% 141 96431 0.94% 142 101449 0.99% 143 111670 1.09% 144 122775 1.20% 145 131528 1.29% 146 157437 1.54% 147 192459 1.88% 148 265710 2.60% 149 489758 4.79% 150 1901063 18.59% 151 3539091 34.61% criterion=sequence-density sequence-density=5.73 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=26 prefix-density=5.77 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.45 sequence-density-rank=18 fanout-score=28.63 fanout-score-rank=1 prefix-density=12.92 prefix-fanout=1.0 sequence=CGGTGTGTACAGGGCCCGGGTACATATTCACCGCGGCATGCTGATCCGCGATTACTAG criterion=sequence-density sequence-density=4.45 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=28 prefix-density=4.52 prefix-fanout=2.0 sequence=CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGGCGATGC criterion=fanout-score sequence-density=0.37 sequence-density-rank=17 fanout-score=22.52 fanout-score-rank=1 prefix-density=3.71 prefix-fanout=2.2 sequence=GACCGATAGCGTACAAGTACCGTGAG SRR6257525 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 09:05:32 Started mapping on | Dec 07 09:05:32 Finished on | Dec 07 09:09:15 Mapping speed, Million of reads per hour | 247.63 Number of input reads | 15339137 Average input read length | 279 UNIQUE READS: Uniquely mapped reads number | 2833084 Uniquely mapped reads % | 18.47% Average mapped length | 283.47 Number of splices: Total | 111085 Number of splices: Annotated (sjdb) | 92321 Number of splices: GT/AG | 102613 Number of splices: GC/AG | 1695 Number of splices: AT/AC | 126 Number of splices: Non-canonical | 6651 Mismatch rate per base, % | 0.26% Deletion rate per base | 0.05% Deletion average length | 2.86 Insertion rate per base | 0.00% Insertion average length | 1.56 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2347691 % of reads mapped to multiple loci | 15.31% Number of reads mapped to too many loci | 2486221 % of reads mapped to too many loci | 16.21% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.38% % of reads unmapped: other | 41.63% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10159683 10159683 10159683 N_multimapping 2347691 2347691 2347691 N_noFeature 2164493 2724275 2264833 N_ambiguous 72110 1060 63207 UnstrandedReadsAssigned:596481 PositiveStrandReadsAssigned:107749 NegativeStrandReadsAssigned:505044 Dataset is classified unstranded MeadianReadLen=150 20thPercentileLength=127 echo kmer=123 SRR6257525 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6257525-trimmed-pair1.fastq SRR6257525-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,339,137 reads, 2,931,850 reads pseudoaligned [quant] estimated average fragment length: 180.106 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 808 rounds 52973 SRR6257525.ke.tsv 35125 SRR6257525.se.tsv 88098 total ==> SRR6257525.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 756.894 0 0 PNS24247 1044 864.894 0 0 PNS24249 1928 1748.89 0 0 PNS24246 1044 864.894 0 0 PNS24248 1044 864.894 0 0 PNS24244 1471 1291.89 9 2.04474 PNS24243 293 115.716 0 0 KQK14069 1603 1423.89 68.5759 14.1356 KQK14071 474 294.989 10.1756 10.1245 ==> SRR6257525.se.tsv <== BRADI_1g14170v3 80 BRADI_1g53295v3 13 BRADI_1g59795v3 3 BRADI_1g07683v3 0 BRADI_1g00485v3 8 BRADI_1g20270v3 14 BRADI_1g74790v3 2 BRADI_1g09890v3 0 BRADI_1g77505v3 13 BRADI_1g48960v3 1 SRR6257525 completed mapping pipeline successfully