Starting /dee2/code/volunteer_pipeline.sh SRR6257526 current disk space = 1544296562688 free memory = 1598317796 SRR6257526 SRAfilesize f0dd6d17be5a4af70aab2fed641a02de SRR6257526.sra SRR6257526.sra file validated SRR6257526 is paired end SRR6257526 is conventional basespace SRR6257526 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6257526_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.8505 28.0 18.0 32.0 18.0 33.0 2 24.0525 25.0 18.0 29.0 18.0 33.0 3 26.3735 27.0 25.0 31.0 18.0 31.0 4 28.10125 29.0 27.0 31.0 25.0 33.0 5 30.12525 31.0 29.0 33.0 27.0 33.0 6 33.87975 36.0 33.0 37.0 28.0 38.0 7 35.13775 37.0 35.0 38.0 30.0 38.0 8 36.82575 38.0 37.0 38.0 35.0 38.0 9 37.1005 38.0 38.0 38.0 36.0 38.0 10-14 37.385450000000006 38.0 38.0 38.0 36.8 38.0 15-19 37.556650000000005 38.0 38.0 38.0 37.8 38.0 20-24 37.397850000000005 38.0 38.0 38.0 37.2 38.0 25-29 36.53745 38.0 37.4 38.0 33.4 38.0 30-34 37.2488 38.0 38.0 38.0 36.6 38.0 35-39 37.394949999999994 38.0 38.0 38.0 37.0 38.0 40-44 36.545750000000005 38.0 37.4 38.0 31.8 38.0 45-49 37.0269 38.0 38.0 38.0 35.8 38.0 50-54 37.108700000000006 38.0 38.0 38.0 36.0 38.0 55-59 37.18605 38.0 38.0 38.0 36.0 38.0 60-64 37.0505 38.0 38.0 38.0 35.6 38.0 65-69 37.12975 38.0 38.0 38.0 36.0 38.0 70-74 36.39725 38.0 37.4 38.0 33.0 38.0 75-79 34.8549 37.2 32.8 38.0 29.2 38.0 80-84 35.50285 37.8 35.2 38.0 30.8 38.0 85-89 35.40509999999999 38.0 35.8 38.0 28.8 38.0 90-94 36.206849999999996 38.0 37.2 38.0 33.2 38.0 95-99 36.3325 38.0 37.8 38.0 33.6 38.0 100-104 36.09825 38.0 37.0 38.0 33.0 38.0 105-109 34.52475 38.0 34.6 38.0 24.8 38.0 110-114 35.3971 38.0 35.6 38.0 30.2 38.0 115-119 35.02265 38.0 35.0 38.0 28.2 38.0 120-124 34.7766 38.0 35.0 38.0 27.2 38.0 125-129 34.44905 38.0 34.4 38.0 25.4 38.0 130-134 33.95625 38.0 34.4 38.0 23.8 38.0 135-139 30.245549999999998 33.8 26.0 37.8 16.4 38.0 140-144 32.77035 36.8 33.2 38.0 15.4 38.0 145-149 32.114050000000006 37.2 33.0 38.0 11.4 38.0 150-151 27.521 35.5 17.0 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 0.0 16 0.0 17 4.0 18 7.0 19 5.0 20 5.0 21 4.0 22 8.0 23 7.0 24 13.0 25 9.0 26 21.0 27 25.0 28 35.0 29 44.0 30 77.0 31 81.0 32 143.0 33 224.0 34 347.0 35 704.0 36 1466.0 37 767.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.42194992008525 7.911561001598296 3.702717101758125 33.96377197655834 2 24.95 8.375 31.474999999999998 35.199999999999996 3 24.85 14.249999999999998 21.75 39.15 4 28.65 19.7 18.275 33.375 5 30.225 22.625 21.575 25.575 6 26.863431715857928 25.68784392196098 22.236118059029515 25.212606303151574 7 19.7 17.974999999999998 40.6 21.725 8 23.7 18.8 26.950000000000003 30.55 9 22.5 16.6 31.874999999999996 29.025000000000002 10-14 25.995 20.915 24.505 28.585 15-19 24.51 20.685000000000002 26.029999999999998 28.775000000000002 20-24 24.21 21.15 24.22 30.42 25-29 23.369999999999997 19.994999999999997 24.03 32.605000000000004 30-34 26.33 18.834999999999997 23.895 30.94 35-39 25.515 21.23 25.1 28.155 40-44 27.605 20.21 23.615 28.57 45-49 26.02 19.650000000000002 25.935000000000002 28.395 50-54 25.080000000000002 19.28 25.095 30.545 55-59 24.705 18.775 25.53 30.990000000000002 60-64 24.9 18.73 25.94 30.43 65-69 23.9 22.0 24.395 29.705 70-74 24.825 21.5 23.645 30.03 75-79 25.41 21.235 25.374999999999996 27.98 80-84 23.27 21.095 25.685000000000002 29.95 85-89 24.43 21.115000000000002 23.43 31.025000000000002 90-94 24.63 20.89 24.12 30.36 95-99 23.9 20.66 24.82 30.620000000000005 100-104 23.505000000000003 20.86 23.98 31.655 105-109 22.795 22.49 24.01 30.705 110-114 23.095 23.5 23.935000000000002 29.470000000000002 115-119 21.87 23.375 23.974999999999998 30.78 120-124 23.14 23.49 23.59 29.78 125-129 22.955000000000002 23.294999999999998 22.564999999999998 31.185000000000002 130-134 23.24 22.725 21.3 32.735 135-139 21.18 24.62 22.63 31.569999999999997 140-144 22.134999999999998 23.905 21.87 32.09 145-149 23.385 23.21 23.25 30.154999999999998 150-151 21.562734785875282 24.61808164287503 21.27473077886301 32.54445279238667 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.5 24 0.5 25 0.5 26 0.5 27 0.5 28 1.0 29 2.5 30 3.5 31 3.0 32 5.0 33 5.5 34 3.5 35 4.0 36 7.0 37 13.5 38 18.0 39 20.0 40 25.0 41 24.5 42 22.5 43 25.0 44 31.0 45 35.5 46 49.0 47 77.0 48 117.0 49 141.0 50 180.5 51 236.5 52 233.5 53 232.5 54 243.5 55 239.0 56 235.5 57 226.0 58 216.5 59 210.5 60 195.0 61 179.5 62 157.5 63 140.0 64 115.5 65 67.0 66 34.5 67 28.5 68 28.5 69 22.5 70 21.0 71 17.0 72 10.5 73 6.5 74 7.0 75 12.0 76 24.0 77 25.5 78 13.0 79 3.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 6.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.05 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.17500000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.175 #Duplication Level Percentage of deduplicated Percentage of total 1 76.1495487752471 44.3 2 11.345079501504083 13.200000000000001 3 4.340352385045122 7.575 4 2.2776106574989257 5.3 5 2.0627417275461966 6.0 6 1.0743446497636442 3.75 7 0.4727116458960034 1.925 8 0.6016330038676407 2.8000000000000003 9 0.21486892995272885 1.125 >10 1.461108723678556 14.025000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 41 1.0250000000000001 No Hit CCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGC 39 0.975 No Hit GCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 38 0.95 No Hit CGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATT 34 0.8500000000000001 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 30 0.75 No Hit GTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGAC 22 0.5499999999999999 No Hit GCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTT 20 0.5 No Hit CCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT 18 0.44999999999999996 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 17 0.42500000000000004 No Hit GTGCGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCC 15 0.375 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 15 0.375 No Hit GTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGA 15 0.375 No Hit GTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGT 14 0.35000000000000003 No Hit GTCACTTTCGTGTACCCATCGGACGGCAGCCCTTTCGGGGGTTCCTTAGG 13 0.325 No Hit GTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACAC 13 0.325 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 13 0.325 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 13 0.325 No Hit GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC 13 0.325 No Hit GTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGC 13 0.325 No Hit ATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAA 12 0.3 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 12 0.3 No Hit GCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGTTG 12 0.3 No Hit GTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAG 12 0.3 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 12 0.3 No Hit GCCCCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGG 12 0.3 No Hit GCGCTATCTGCCGGGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGTG 11 0.27499999999999997 No Hit GGGTAATGACTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTG 11 0.27499999999999997 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 11 0.27499999999999997 No Hit GGCCGTTGCAGCGCAGTGCCCCGAGGGACACGCCTTGCGGCGCGCGGGTA 10 0.25 No Hit GTCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 10 0.25 No Hit GTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCC 10 0.25 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 10 0.25 No Hit GTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGCAGC 10 0.25 No Hit CGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGG 10 0.25 No Hit CCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAAACCTTGGGTTTT 9 0.22499999999999998 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 9 0.22499999999999998 No Hit GGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGGCGTCTC 9 0.22499999999999998 No Hit CCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCG 9 0.22499999999999998 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 9 0.22499999999999998 No Hit GCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCC 8 0.2 No Hit ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG 8 0.2 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 8 0.2 No Hit CCTTGTTCCGCTCTCGCTCGCCGCTACTAACGGGGTCTCGGTTGATTTTC 8 0.2 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 8 0.2 No Hit GGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCT 8 0.2 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 8 0.2 No Hit GTAATGACTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTA 8 0.2 No Hit GGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGG 8 0.2 No Hit GGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACACCT 8 0.2 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 8 0.2 No Hit GGGTCACCTTGTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCC 8 0.2 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 8 0.2 No Hit GTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA 8 0.2 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 7 0.17500000000000002 No Hit GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG 7 0.17500000000000002 No Hit ATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAAC 7 0.17500000000000002 No Hit GCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGG 7 0.17500000000000002 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 7 0.17500000000000002 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 7 0.17500000000000002 No Hit GCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGC 7 0.17500000000000002 No Hit GGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTG 7 0.17500000000000002 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 7 0.17500000000000002 No Hit CGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGG 7 0.17500000000000002 No Hit CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC 7 0.17500000000000002 No Hit GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTA 6 0.15 No Hit GTCACCTTGTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTC 6 0.15 No Hit GTTTGGGGCCGGGACCCCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCC 6 0.15 No Hit CCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGT 6 0.15 No Hit GCAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG 6 0.15 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC 6 0.15 No Hit GGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTT 6 0.15 No Hit GCCGTTCACATGGAACCTTTCTCCTCTTCGGCCTTCAAAGTTCTCATTTG 6 0.15 No Hit CACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTT 6 0.15 No Hit CGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTA 6 0.15 No Hit GCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGG 6 0.15 No Hit GGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCGCGCGCTTTAGCG 6 0.15 No Hit GCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTA 6 0.15 No Hit GCTCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 6 0.15 No Hit CAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGA 6 0.15 No Hit CCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTA 6 0.15 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 6 0.15 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 6 0.15 No Hit GCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCGGCCAGCGGTGCGGC 6 0.15 No Hit CCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAA 6 0.15 No Hit GTCCCGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCG 6 0.15 No Hit GGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCG 6 0.15 No Hit GGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGA 6 0.15 No Hit GTCGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCAACCATCTCGTAT 6 0.15 TruSeq Adapter, Index 10 (97% over 37bp) GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 5 0.125 No Hit ATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGC 5 0.125 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 5 0.125 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 5 0.125 No Hit AGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGC 5 0.125 No Hit CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC 5 0.125 No Hit CAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCT 5 0.125 No Hit CCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGG 5 0.125 No Hit GATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCA 5 0.125 No Hit GTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTG 5 0.125 No Hit CACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCAGCGCAAGGGCGC 5 0.125 No Hit GGGGTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGC 5 0.125 No Hit GGGAGCTCAGCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCT 5 0.125 No Hit CCGAGGAAACCTTTGCACGCCTCCGTTACCTTTTGGGAGGCCTACGCCCC 5 0.125 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 5 0.125 No Hit GGGGCCGGGACCCCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCCGAAG 5 0.125 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 5 0.125 No Hit CCACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCAGCGCAAGGGCG 5 0.125 No Hit CTCAGCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCA 5 0.125 No Hit GTCAGTATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGC 5 0.125 No Hit CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT 5 0.125 No Hit GGTAATGACTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGT 5 0.125 No Hit CCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGA 5 0.125 No Hit GCCCGAGGAAACCTTTGCACGCCTCCGTTACCTTTTGGGAGGCCTACGCC 5 0.125 No Hit CCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGT 5 0.125 No Hit CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAG 5 0.125 No Hit GTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC 5 0.125 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 5 0.125 No Hit CCCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGGCT 5 0.125 No Hit GTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGC 5 0.125 No Hit CCGGCGATTACTAGCGATTCCTGCTTCATGCAGGCGAGTTGCAGCCTGCA 5 0.125 No Hit GCGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAG 5 0.125 No Hit CACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCAC 5 0.125 No Hit GTGCCGTTCACATGGAACCTTTCTCCTCTTCGGCCTTCAAAGTTCTCATT 5 0.125 No Hit CCAGGGTTGGCAGGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCC 5 0.125 No Hit CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 5 0.125 No Hit GCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGCC 5 0.125 No Hit CAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGT 5 0.125 No Hit GGGTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGCA 5 0.125 No Hit GTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCT 5 0.125 No Hit GTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTA 5 0.125 No Hit ATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTG 5 0.125 No Hit AGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGATTCA 5 0.125 No Hit GGTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGCAG 5 0.125 No Hit CCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGGCTC 5 0.125 No Hit GCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGT 5 0.125 No Hit CGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTG 5 0.125 No Hit GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.0625 0.0 0.0 0.0 0.0 62-63 0.11249999999999999 0.0 0.0 0.0 0.0 64-65 0.21250000000000002 0.0 0.0 0.0 0.0 66-67 0.275 0.0 0.0 0.0 0.0 68-69 0.3875 0.0 0.0 0.0 0.0 70-71 0.44999999999999996 0.0 0.0 0.0 0.0 72-73 0.6375 0.0 0.0 0.0 0.0 74-75 0.7375 0.0 0.0 0.0 0.0 76-77 0.8999999999999999 0.0 0.0 0.0 0.0 78-79 1.0625 0.0 0.0 0.0 0.0 80-81 1.275 0.0 0.0 0.0 0.0 82-83 1.525 0.0 0.0 0.0 0.0 84-85 1.8125 0.0 0.0 0.0 0.0 86-87 2.0250000000000004 0.0 0.0 0.0 0.0 88-89 2.4375 0.0 0.0 0.0 0.0 90-91 2.8625 0.0 0.0 0.0 0.0 92-93 3.5125 0.0 0.0 0.0 0.0 94-95 4.1 0.0 0.0 0.0 0.0 96-97 4.8 0.0 0.0 0.0 0.0 98-99 5.625 0.0 0.0 0.0 0.0 100-101 6.6125 0.0 0.0 0.0 0.0 102-103 7.5 0.0 0.0 0.0 0.0 104-105 8.3375 0.0 0.0 0.0 0.0 106-107 9.0 0.0 0.0 0.0 0.0 108-109 9.5625 0.0 0.0 0.0 0.0 110-111 10.425 0.0 0.0 0.0 0.0 112-113 11.4125 0.0 0.0 0.0 0.0 114-115 12.662500000000001 0.0 0.0 0.0 0.0 116-117 13.95 0.0 0.0 0.0 0.0 118-119 14.8375 0.0 0.0 0.0 0.0 120-121 15.9 0.0 0.0 0.0 0.0 122-123 16.9875 0.0 0.0 0.0 0.0 124-125 18.3125 0.0 0.0 0.0 0.0 126-127 19.3875 0.0 0.0 0.0 0.0 128-129 20.0875 0.0 0.0 0.0 0.0 130-131 20.9125 0.0 0.0 0.0 0.0 132-133 22.2 0.0 0.0 0.0 0.0 134-135 23.05 0.0 0.0 0.0 0.0 136-137 24.0875 0.0 0.0 0.0 0.0 138-139 25.4375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGTCC 10 0.0068378756 144.95 145 GTAGGCC 25 8.7252335E-4 86.97 145 TTGTTAT 30 0.0017998101 72.475006 6 GTATTGT 30 0.0017998101 72.475006 3 TATTGTT 30 0.0017998101 72.475006 4 ATTGTTA 30 0.0017998101 72.475006 5 GTTATTT 35 0.0033169514 62.121426 8 TTATTTA 40 0.0056290138 54.35625 9 TGTTATT 40 0.0056290138 54.35625 7 CTCCCTC 30 0.0014466991 24.158335 95-99 CCTAATT 30 0.0014466991 24.158335 115-119 TCAGGAT 30 0.0014466991 24.158335 35-39 CTAATTC 30 0.0014466991 24.158335 115-119 GAATCGA 30 0.0014466991 24.158335 105-109 CGTGTCA 30 0.0014466991 24.158335 30-34 CAGGCTC 30 0.0014466991 24.158335 90-94 CAGGATT 30 0.0014466991 24.158335 35-39 GGAATCG 30 0.0014466991 24.158335 105-109 TTGTCAC 35 0.00354369 20.707142 9 TGCGCGC 35 0.00354369 20.707142 50-54 >>END_MODULE SRR6257526 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6257526_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07725 33.0 32.0 33.0 28.0 34.0 2 32.498 33.0 33.0 34.0 32.0 34.0 3 32.62525 33.0 33.0 34.0 32.0 34.0 4 32.74475 33.0 33.0 34.0 32.0 34.0 5 28.53525 33.0 27.0 33.0 15.0 34.0 6 35.41025 38.0 36.0 38.0 29.0 38.0 7 36.53975 38.0 37.0 38.0 34.0 38.0 8 36.94675 38.0 38.0 38.0 36.0 38.0 9 36.966 38.0 38.0 38.0 36.0 38.0 10-14 34.878600000000006 37.4 32.2 38.0 28.8 38.0 15-19 34.79275 38.0 34.2 38.0 25.4 38.0 20-24 37.060199999999995 38.0 38.0 38.0 36.6 38.0 25-29 37.07940000000001 38.0 38.0 38.0 36.8 38.0 30-34 36.834700000000005 38.0 38.0 38.0 35.8 38.0 35-39 36.99425 38.0 38.0 38.0 35.8 38.0 40-44 37.00995 38.0 38.0 38.0 36.2 38.0 45-49 36.709399999999995 38.0 38.0 38.0 35.0 38.0 50-54 36.615700000000004 38.0 38.0 38.0 34.8 38.0 55-59 36.72495 38.0 38.0 38.0 35.0 38.0 60-64 35.36715 38.0 35.6 38.0 29.0 38.0 65-69 36.2191 38.0 37.8 38.0 33.0 38.0 70-74 36.71025 38.0 38.0 38.0 35.0 38.0 75-79 35.92165 38.0 37.2 38.0 31.2 38.0 80-84 36.37 38.0 37.8 38.0 33.8 38.0 85-89 35.61135 38.0 36.8 38.0 29.2 38.0 90-94 35.782 38.0 37.0 38.0 32.2 38.0 95-99 31.40625 34.2 25.6 38.0 22.8 38.0 100-104 34.72580000000001 37.4 34.0 38.0 28.8 38.0 105-109 35.86964999999999 38.0 37.0 38.0 32.6 38.0 110-114 35.36005 38.0 35.8 38.0 31.2 38.0 115-119 35.039199999999994 38.0 35.0 38.0 28.6 38.0 120-124 34.94525 38.0 35.0 38.0 28.8 38.0 125-129 33.48085 38.0 32.8 38.0 23.2 38.0 130-134 30.7485 34.6 25.4 38.0 16.2 38.0 135-139 32.5448 37.6 31.8 38.0 16.4 38.0 140-144 31.922949999999997 38.0 31.4 38.0 12.6 38.0 145-149 30.303800000000003 36.4 29.2 38.0 6.0 38.0 150-151 24.425375 31.0 14.0 36.5 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 3.0 4 3.0 5 0.0 6 1.0 7 0.0 8 0.0 9 0.0 10 1.0 11 3.0 12 2.0 13 1.0 14 4.0 15 0.0 16 3.0 17 12.0 18 3.0 19 7.0 20 6.0 21 6.0 22 20.0 23 13.0 24 15.0 25 23.0 26 33.0 27 37.0 28 36.0 29 47.0 30 82.0 31 91.0 32 171.0 33 213.0 34 336.0 35 682.0 36 1308.0 37 829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.025 13.55 6.2 25.224999999999998 2 35.775 17.625 21.825 24.775 3 28.7 23.974999999999998 22.05 25.275 4 31.05 30.375000000000004 18.4 20.175 5 29.7 30.325000000000003 17.375 22.6 6 29.725 33.900000000000006 16.125 20.25 7 27.325 18.025 33.300000000000004 21.349999999999998 8 27.875 18.6 25.124999999999996 28.4 9 27.800000000000004 21.725 23.549999999999997 26.924999999999997 10-14 29.275000000000002 25.1 22.314999999999998 23.31 15-19 29.23 23.775 24.415 22.58 20-24 30.599999999999998 24.44 21.675 23.285 25-29 29.73594718943789 24.77995599119824 21.629325865173037 23.854770954190837 30-34 32.234835225283796 24.138620793118967 20.65809871480722 22.96844526679002 35-39 33.05326331582896 25.501375343835956 20.300075018754686 21.145286321580397 40-44 33.445 24.315 21.125 21.115000000000002 45-49 31.71 25.34 19.13 23.82 50-54 29.646482324116207 26.23131156557828 19.440972048602433 24.681234061703087 55-59 30.930000000000003 24.915000000000003 21.68 22.475 60-64 32.574999999999996 23.044999999999998 21.315 23.064999999999998 65-69 32.32161608080404 23.181159057952897 20.061003050152507 24.436221811090554 70-74 32.50162508125406 25.716285814290714 17.42587129356468 24.356217810890545 75-79 30.788078807880787 26.137613761376137 20.337033703370334 22.737273727372738 80-84 32.47 23.549999999999997 19.009999999999998 24.97 85-89 30.81654082704135 24.366218310915546 19.625981299064954 25.191259562978146 90-94 32.37985697854678 23.59353903085463 20.0880132019803 23.938590788618292 95-99 29.695 26.13 20.235 23.94 100-104 30.490000000000002 24.825 20.87 23.815 105-109 29.215000000000003 23.68 22.595000000000002 24.51 110-114 29.9 27.255000000000003 18.11 24.735 115-119 31.125000000000004 23.330000000000002 20.474999999999998 25.069999999999997 120-124 30.125 26.105 19.75 24.02 125-129 30.025000000000002 26.25 19.994999999999997 23.73 130-134 31.061212242448487 26.410282056411283 19.02880576115223 23.499699939987998 135-139 31.909786467970196 24.323648547282094 19.092863929589438 24.673701055158272 140-144 33.081654082704134 24.456222811140556 19.29596479823991 23.166158307915396 145-149 31.45 26.740000000000002 20.064999999999998 21.745 150-151 31.158422041327487 27.48904195366312 21.302442078897936 20.050093926111458 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 0.5 25 0.0 26 0.0 27 0.0 28 0.5 29 2.0 30 3.0 31 2.5 32 5.0 33 5.5 34 4.5 35 10.0 36 11.0 37 11.0 38 8.5 39 5.0 40 13.0 41 18.0 42 16.5 43 34.0 44 43.0 45 43.5 46 74.5 47 126.5 48 178.0 49 207.5 50 200.5 51 212.5 52 231.5 53 231.5 54 259.0 55 269.5 56 235.0 57 197.0 58 176.0 59 149.5 60 133.0 61 132.5 62 122.5 63 87.5 64 56.0 65 49.5 66 51.5 67 58.0 68 61.5 69 46.5 70 39.5 71 38.0 72 20.0 73 12.5 74 15.0 75 25.0 76 30.0 77 21.0 78 10.0 79 3.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.02 30-34 0.015 35-39 0.025 40-44 0.0 45-49 0.0 50-54 0.005 55-59 0.0 60-64 0.0 65-69 0.005 70-74 0.005 75-79 0.01 80-84 0.0 85-89 0.005 90-94 0.015 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.02 135-139 0.015 140-144 0.005 145-149 0.0 150-151 0.1875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.75 #Duplication Level Percentage of deduplicated Percentage of total 1 84.91289198606272 60.925 2 8.710801393728223 12.5 3 2.9268292682926833 6.3 4 0.8710801393728222 2.5 5 0.5923344947735192 2.125 6 0.5923344947735192 2.55 7 0.2787456445993031 1.4000000000000001 8 0.06968641114982578 0.4 9 0.13937282229965156 0.8999999999999999 >10 0.9059233449477353 10.4 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCC 42 1.05 No Hit CCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATC 24 0.6 No Hit GCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATA 23 0.575 No Hit CCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGAC 23 0.575 No Hit GCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGC 22 0.5499999999999999 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 22 0.5499999999999999 No Hit CGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCA 22 0.5499999999999999 No Hit CATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCT 20 0.5 No Hit GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGT 16 0.4 No Hit CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAAC 15 0.375 No Hit GGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAA 15 0.375 No Hit AGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGA 14 0.35000000000000003 No Hit GGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAA 14 0.35000000000000003 No Hit CTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCA 13 0.325 No Hit GACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCAT 12 0.3 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 12 0.3 No Hit GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC 12 0.3 No Hit GCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAA 12 0.3 No Hit CCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAAT 11 0.27499999999999997 No Hit GGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCG 11 0.27499999999999997 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 11 0.27499999999999997 No Hit GGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAAC 10 0.25 No Hit GGGGCTTTCCCCGAGCGCTGAACAGTCGACTCAGAACTGGTACGGACAAG 10 0.25 No Hit GTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACG 10 0.25 No Hit GGGAGCGTTCCGCCTTAGAGGGAAGCAACCGCGAAAGCGGGGGTCGACGA 10 0.25 No Hit AGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAA 10 0.25 No Hit GGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCA 9 0.22499999999999998 No Hit GGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAG 9 0.22499999999999998 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 9 0.22499999999999998 No Hit GCATAATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCC 9 0.22499999999999998 No Hit GCATCATTCAACTTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCC 8 0.2 No Hit GGAGCGTTCCGCCTTAGAGGGAAGCAACCGCGAAAGCGGGGGTCGACGAA 8 0.2 No Hit GCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCGC 7 0.17500000000000002 No Hit AGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAAT 7 0.17500000000000002 No Hit GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT 7 0.17500000000000002 No Hit GTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCCCCCGG 7 0.17500000000000002 No Hit GCGGGACGTTCTGTCAATCGGGGAAGGTTTTTGGTGACAAGACCTGGAGA 7 0.17500000000000002 No Hit TGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACGGA 7 0.17500000000000002 No Hit CGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATG 7 0.17500000000000002 No Hit CGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACG 7 0.17500000000000002 No Hit AGGGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGC 6 0.15 No Hit GTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCGCCTCG 6 0.15 No Hit CATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGG 6 0.15 No Hit GAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGG 6 0.15 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 6 0.15 No Hit GGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAG 6 0.15 No Hit GCTAGCTTCTTAGAGTGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGC 6 0.15 No Hit GGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGAT 6 0.15 No Hit GTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATG 6 0.15 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 6 0.15 No Hit GCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTT 6 0.15 No Hit CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA 6 0.15 No Hit CATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAACAA 6 0.15 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 6 0.15 No Hit GTTGAAGAATCAGCGGATGAGTTGTGGTTAGGGGTGAAATGCCACTCGAA 6 0.15 No Hit GCTAGGCGAAGCGGTTGAGTGCCGCACCCTAGATGGCTAAAGTCCAGTAG 6 0.15 No Hit GACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCAT 6 0.15 No Hit CATCATTCAAATTTCGGCCCTATCAACTTTCGATGGTAGGATAGGGGCCT 5 0.125 No Hit CTCATAACCGCATCAGTTCTCCAAGGTGAACAGCCTCTGGCCAATGGAAC 5 0.125 No Hit GGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGAC 5 0.125 No Hit GGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGT 5 0.125 No Hit GTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGG 5 0.125 No Hit CACCGCTGGCCGACCCTGATCTTCTGTGAAGGGTTCGAGTTGGAGCACGC 5 0.125 No Hit GAGCGTTCCGCCTTAGAGGGAAGCAACCGCGAAAGCGGGGGTCGACGAAG 5 0.125 No Hit GGACTGTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCC 5 0.125 No Hit GCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCC 5 0.125 No Hit ATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACGTTA 5 0.125 No Hit GTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAG 5 0.125 No Hit GTCTGACATGCGTGCTAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 5 0.125 No Hit GCGAAAGCGAGTCTTCATAGGGCGATTGTCACTGCTTATGGACCCGAACC 5 0.125 No Hit GTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTC 5 0.125 No Hit TGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTAT 5 0.125 No Hit CAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATC 5 0.125 No Hit GGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.0625 0.0 0.0 0.0 0.0 62-63 0.11249999999999999 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.325 0.0 0.0 0.0 0.0 68-69 0.44999999999999996 0.0 0.0 0.0 0.0 70-71 0.5 0.0 0.0 0.0 0.0 72-73 0.7375 0.0 0.0 0.0 0.0 74-75 0.875 0.0 0.0 0.0 0.0 76-77 1.075 0.0 0.0 0.0 0.0 78-79 1.2625000000000002 0.0 0.0 0.0 0.0 80-81 1.525 0.0 0.0 0.0 0.0 82-83 1.775 0.0 0.0 0.0 0.0 84-85 2.0375 0.0 0.0 0.0 0.0 86-87 2.2125 0.0 0.0 0.0 0.0 88-89 2.4875 0.0 0.0 0.0 0.0 90-91 2.7375 0.0 0.0 0.0 0.0 92-93 3.1625 0.0 0.0 0.0 0.0 94-95 3.6375 0.0 0.0 0.0 0.0 96-97 4.050000000000001 0.0 0.0 0.0 0.0 98-99 4.725 0.0 0.0 0.0 0.0 100-101 5.699999999999999 0.0 0.0 0.0 0.0 102-103 6.7 0.0 0.0 0.0 0.0 104-105 7.5375 0.0 0.0 0.0 0.0 106-107 8.2375 0.0 0.0 0.0 0.0 108-109 8.8125 0.0 0.0 0.0 0.0 110-111 9.675 0.0 0.0 0.0 0.0 112-113 10.649999999999999 0.0 0.0 0.0 0.0 114-115 11.9 0.0 0.0 0.0 0.0 116-117 13.1875 0.0 0.0 0.0 0.0 118-119 13.95 0.0 0.0 0.0 0.0 120-121 14.7625 0.0 0.0 0.0 0.0 122-123 15.5625 0.0 0.0 0.0 0.0 124-125 16.5375 0.0 0.0 0.0 0.0 126-127 17.625 0.0 0.0 0.0 0.0 128-129 18.450000000000003 0.0 0.0 0.0 0.0 130-131 19.5125 0.0 0.0 0.0 0.0 132-133 20.9625 0.0 0.0 0.0 0.0 134-135 22.05 0.0 0.0 0.0 0.0 136-137 23.299999999999997 0.0 0.0 0.0 0.0 138-139 24.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTTCT 10 0.006830828 145.0 4 TAGCTTC 10 0.006830828 145.0 3 CATCATT 15 1.1411342E-4 145.0 2 ATCATTC 15 1.1411342E-4 145.0 3 TCATTCA 15 1.1411342E-4 145.0 4 GCATCAT 15 1.1411342E-4 145.0 1 CATTCAA 20 3.5877043E-4 108.75 5 GCAAATT 35 0.0033124194 62.14286 145 CCCTATC 20 0.00593511 29.0 15-19 TTTCGAT 20 0.00593511 29.0 25-29 >>END_MODULE Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804312 spots for SRR6257526.sra Written 804312 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra Read 804295 spots for SRR6257526.sra Written 804295 spots for SRR6257526.sra SRR ids: ['SRR6257526.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7j80u7i0 SRR6257526.sra spots: 16085917 blocks: [[1, 804295], [804296, 1608590], [1608591, 2412885], [2412886, 3217180], [3217181, 4021475], [4021476, 4825770], [4825771, 5630065], [5630066, 6434360], [6434361, 7238655], [7238656, 8042950], [8042951, 8847245], [8847246, 9651540], [9651541, 10455835], [10455836, 11260130], [11260131, 12064425], [12064426, 12868720], [12868721, 13673015], [13673016, 14477310], [14477311, 15281605], [15281606, 16085917]] SRR6257526 file size 5429289 SRR6257526 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6257526 SRR6257526_1.fastq SRR6257526_2.fastq Input file: SRR6257526_1.fastq Paired file: SRR6257526_2.fastq trimmed: SRR6257526-trimmed-pair1.fastq, SRR6257526-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 09:03:55 2024 >> started Sat Dec 7 09:04:12 2024 >> done (17.488s) 16085917 read pairs processed; of these: 15940 ( 0.10%) short read pairs filtered out after trimming by size control 32311 ( 0.20%) empty read pairs filtered out after trimming by size control 16037666 (99.70%) read pairs available; of these: 11063740 (68.99%) trimmed read pairs available after processing 4973926 (31.01%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 17 0.00% 20 15 0.00% 21 21 0.00% 22 27 0.00% 23 39 0.00% 24 29 0.00% 25 51 0.00% 26 85 0.00% 27 98 0.00% 28 120 0.00% 29 117 0.00% 30 175 0.00% 31 143 0.00% 32 164 0.00% 33 193 0.00% 34 220 0.00% 35 251 0.00% 36 269 0.00% 37 298 0.00% 38 360 0.00% 39 531 0.00% 40 516 0.00% 41 609 0.00% 42 593 0.00% 43 583 0.00% 44 615 0.00% 45 726 0.00% 46 856 0.01% 47 1065 0.01% 48 1239 0.01% 49 1416 0.01% 50 1508 0.01% 51 1615 0.01% 52 1661 0.01% 53 1801 0.01% 54 1971 0.01% 55 1872 0.01% 56 2084 0.01% 57 2413 0.02% 58 2752 0.02% 59 3065 0.02% 60 3532 0.02% 61 4819 0.03% 62 4741 0.03% 63 5790 0.04% 64 5939 0.04% 65 5952 0.04% 66 6768 0.04% 67 7728 0.05% 68 8286 0.05% 69 8835 0.06% 70 10094 0.06% 71 11165 0.07% 72 12932 0.08% 73 14098 0.09% 74 15067 0.09% 75 14915 0.09% 76 14611 0.09% 77 14466 0.09% 78 15905 0.10% 79 19131 0.12% 80 20652 0.13% 81 21572 0.13% 82 24125 0.15% 83 25703 0.16% 84 25866 0.16% 85 27556 0.17% 86 28247 0.18% 87 31007 0.19% 88 31433 0.20% 89 31635 0.20% 90 31192 0.19% 91 37294 0.23% 92 41792 0.26% 93 45203 0.28% 94 50980 0.32% 95 52808 0.33% 96 65077 0.41% 97 59808 0.37% 98 77893 0.49% 99 73375 0.46% 100 86169 0.54% 101 77656 0.48% 102 83351 0.52% 103 80428 0.50% 104 79938 0.50% 105 72544 0.45% 106 77601 0.48% 107 78252 0.49% 108 70786 0.44% 109 78922 0.49% 110 81694 0.51% 111 94604 0.59% 112 116233 0.72% 113 93546 0.58% 114 99266 0.62% 115 114819 0.72% 116 107915 0.67% 117 94545 0.59% 118 100865 0.63% 119 105248 0.66% 120 105919 0.66% 121 105247 0.66% 122 131497 0.82% 123 121378 0.76% 124 123160 0.77% 125 120675 0.75% 126 128500 0.80% 127 130037 0.81% 128 111538 0.70% 129 126871 0.79% 130 120822 0.75% 131 118900 0.74% 132 121193 0.76% 133 124837 0.78% 134 122658 0.76% 135 126857 0.79% 136 118394 0.74% 137 129950 0.81% 138 131808 0.82% 139 137519 0.86% 140 148877 0.93% 141 160512 1.00% 142 175237 1.09% 143 169954 1.06% 144 199214 1.24% 145 214922 1.34% 146 253487 1.58% 147 307708 1.92% 148 427111 2.66% 149 792629 4.94% 150 2921761 18.22% 151 4973926 31.01% 16037666 reads passed initial QC criterion=sequence-density sequence-density=6.41 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=24 prefix-density=6.45 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.39 sequence-density-rank=20 fanout-score=23.47 fanout-score-rank=1 prefix-density=9.02 prefix-fanout=1.0 sequence=CGGTGTGTACAGGGCCCGGGTACATATTCACCGCGGCATGCTGATCCGCGATTACTAG criterion=sequence-density sequence-density=5.63 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=28 prefix-density=5.75 prefix-fanout=1.9 sequence=CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=30 fanout-score=37.97 fanout-score-rank=1 prefix-density=1.07 prefix-fanout=1.0 sequence=CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTTGAACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGGAGGGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTTTGCTCGCTGATCCGATGATTCATGATAACTCGACGGATCGCACGGCCCTCGTGCCGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCTACCATGGTGGTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCT Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGC -o SRR6257526 SRR6257526_1.fastq SRR6257526_2.fastq Input file: SRR6257526_1.fastq Paired file: SRR6257526_2.fastq trimmed: SRR6257526-trimmed-pair1.fastq, SRR6257526-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 09:05:39 2024 >> started Sat Dec 7 09:05:52 2024 >> done (12.465s) 11455476 read pairs processed; of these: 661 ( 0.01%) short read pairs filtered out after trimming by size control 1158 ( 0.01%) empty read pairs filtered out after trimming by size control 11453657 (99.98%) read pairs available; of these: 6912 ( 0.06%) trimmed read pairs available after processing 11446745 (99.94%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 13 0.00% 20 11 0.00% 21 17 0.00% 22 19 0.00% 23 27 0.00% 24 25 0.00% 25 41 0.00% 26 57 0.00% 27 68 0.00% 28 79 0.00% 29 84 0.00% 30 127 0.00% 31 87 0.00% 32 110 0.00% 33 127 0.00% 34 155 0.00% 35 179 0.00% 36 198 0.00% 37 212 0.00% 38 267 0.00% 39 372 0.00% 40 359 0.00% 41 439 0.00% 42 424 0.00% 43 423 0.00% 44 445 0.00% 45 538 0.00% 46 619 0.01% 47 763 0.01% 48 896 0.01% 49 1002 0.01% 50 1086 0.01% 51 1119 0.01% 52 1178 0.01% 53 1286 0.01% 54 1434 0.01% 55 1338 0.01% 56 1473 0.01% 57 1749 0.02% 58 1960 0.02% 59 2192 0.02% 60 2477 0.02% 61 3464 0.03% 62 3324 0.03% 63 4120 0.04% 64 4292 0.04% 65 4219 0.04% 66 4913 0.04% 67 5527 0.05% 68 5892 0.05% 69 6288 0.05% 70 7306 0.06% 71 7994 0.07% 72 9225 0.08% 73 9984 0.09% 74 10816 0.09% 75 10684 0.09% 76 10464 0.09% 77 10232 0.09% 78 11367 0.10% 79 13630 0.12% 80 14709 0.13% 81 15318 0.13% 82 17163 0.15% 83 18341 0.16% 84 18445 0.16% 85 19703 0.17% 86 20194 0.18% 87 22058 0.19% 88 22348 0.20% 89 22570 0.20% 90 22265 0.19% 91 26677 0.23% 92 29884 0.26% 93 32273 0.28% 94 36251 0.32% 95 37686 0.33% 96 46585 0.41% 97 42700 0.37% 98 55700 0.49% 99 52352 0.46% 100 61445 0.54% 101 55706 0.49% 102 59601 0.52% 103 57537 0.50% 104 57180 0.50% 105 51848 0.45% 106 55343 0.48% 107 55787 0.49% 108 50255 0.44% 109 56222 0.49% 110 58224 0.51% 111 67574 0.59% 112 82886 0.72% 113 66835 0.58% 114 70882 0.62% 115 82275 0.72% 116 77241 0.67% 117 67359 0.59% 118 71834 0.63% 119 75306 0.66% 120 75704 0.66% 121 75215 0.66% 122 94066 0.82% 123 86499 0.76% 124 87796 0.77% 125 86133 0.75% 126 91656 0.80% 127 92719 0.81% 128 79873 0.70% 129 90660 0.79% 130 86128 0.75% 131 84769 0.74% 132 86805 0.76% 133 89505 0.78% 134 87914 0.77% 135 90592 0.79% 136 84697 0.74% 137 93022 0.81% 138 94121 0.82% 139 98231 0.86% 140 106313 0.93% 141 114835 1.00% 142 125237 1.09% 143 121307 1.06% 144 142459 1.24% 145 153516 1.34% 146 181192 1.58% 147 219945 1.92% 148 305251 2.67% 149 565744 4.94% 150 2084746 18.20% 151 3553222 31.02% criterion=sequence-density sequence-density=6.42 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=25 prefix-density=6.49 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.38 sequence-density-rank=19 fanout-score=23.39 fanout-score-rank=1 prefix-density=8.90 prefix-fanout=1.0 sequence=CGGTGTGTACAGGGCCCGGGTACATATTCACCGCGGCATGCTGATCCGCGATTACTAG criterion=sequence-density sequence-density=5.61 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=28 prefix-density=5.73 prefix-fanout=1.9 sequence=CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=30 fanout-score=38.03 fanout-score-rank=1 prefix-density=1.04 prefix-fanout=1.0 sequence=CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTTGAACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGGAGGGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTTTGCTCGCTGATCCGATGATTCATGATAACTCGACGGATCGCACGGCCCTCGTGCCGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCTACCATGGTGGTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCT SRR6257526 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 09:06:39 Started mapping on | Dec 07 09:06:39 Finished on | Dec 07 09:09:33 Mapping speed, Million of reads per hour | 331.78 Number of input reads | 16035847 Average input read length | 276 UNIQUE READS: Uniquely mapped reads number | 2820519 Uniquely mapped reads % | 17.59% Average mapped length | 281.42 Number of splices: Total | 76947 Number of splices: Annotated (sjdb) | 42219 Number of splices: GT/AG | 51580 Number of splices: GC/AG | 1547 Number of splices: AT/AC | 195 Number of splices: Non-canonical | 23625 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.04% Deletion average length | 2.80 Insertion rate per base | 0.00% Insertion average length | 1.47 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2844164 % of reads mapped to multiple loci | 17.74% Number of reads mapped to too many loci | 2402989 % of reads mapped to too many loci | 14.99% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.40% % of reads unmapped: other | 42.29% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10373652 10373652 10373652 N_multimapping 2844164 2844164 2844164 N_noFeature 2242613 2716473 2340270 N_ambiguous 63935 907 57114 UnstrandedReadsAssigned:513971 PositiveStrandReadsAssigned:103139 NegativeStrandReadsAssigned:423135 Dataset is classified unstranded MeadianReadLen=150 20thPercentileLength=123 echo kmer=119 SRR6257526 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6257526-trimmed-pair1.fastq SRR6257526-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,035,847 reads, 3,360,487 reads pseudoaligned [quant] estimated average fragment length: 175.047 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 800 rounds 52973 SRR6257526.ke.tsv 35125 SRR6257526.se.tsv 88098 total ==> SRR6257526.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 761.963 0 0 PNS24247 1044 869.953 0 0 PNS24249 1928 1753.95 2.37792 0.329394 PNS24246 1044 869.953 0 0 PNS24248 1044 869.953 0 0 PNS24244 1471 1296.95 4.62208 0.865862 PNS24243 293 120.199 2 4.04264 KQK14069 1603 1428.95 20.8644 3.54751 KQK14071 474 299.994 4.72841 3.82947 ==> SRR6257526.se.tsv <== BRADI_1g14170v3 27 BRADI_1g53295v3 9 BRADI_1g59795v3 4 BRADI_1g07683v3 1 BRADI_1g00485v3 7 BRADI_1g20270v3 9 BRADI_1g74790v3 3 BRADI_1g09890v3 0 BRADI_1g77505v3 8 BRADI_1g48960v3 1 SRR6257526 completed mapping pipeline successfully