Starting /dee2/code/volunteer_pipeline.sh SRR6257528 current disk space = 1544259538944 free memory = 1602678640 SRR6257528 SRAfilesize 7e67c2e2c314a78893341751e948fa05 SRR6257528.sra SRR6257528.sra file validated SRR6257528 is paired end SRR6257528 is conventional basespace SRR6257528 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6257528_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.113 18.0 18.0 25.0 18.0 30.0 2 23.868 25.0 18.0 29.0 18.0 30.0 3 25.53425 27.0 18.0 32.0 18.0 32.0 4 28.96475 30.0 28.0 31.0 25.0 33.0 5 31.14625 32.0 32.0 33.0 27.0 33.0 6 35.62775 37.0 36.0 38.0 31.0 38.0 7 37.06825 38.0 37.0 38.0 35.0 38.0 8 37.30525 38.0 38.0 38.0 36.0 38.0 9 37.38875 38.0 38.0 38.0 36.0 38.0 10-14 37.395050000000005 38.0 38.0 38.0 36.8 38.0 15-19 37.45545 38.0 38.0 38.0 37.0 38.0 20-24 37.44175 38.0 38.0 38.0 37.4 38.0 25-29 37.18855 38.0 38.0 38.0 36.8 38.0 30-34 37.1301 38.0 38.0 38.0 36.2 38.0 35-39 37.39075 38.0 38.0 38.0 37.0 38.0 40-44 35.4467 37.8 34.8 38.0 30.0 38.0 45-49 37.03705 38.0 38.0 38.0 35.8 38.0 50-54 36.225049999999996 38.0 36.6 38.0 30.8 38.0 55-59 37.136 38.0 38.0 38.0 36.0 38.0 60-64 36.9461 38.0 38.0 38.0 35.0 38.0 65-69 35.0872 37.6 34.0 38.0 29.0 38.0 70-74 36.911849999999994 38.0 38.0 38.0 35.0 38.0 75-79 36.41965 38.0 37.6 38.0 33.6 38.0 80-84 35.8813 38.0 37.2 38.0 31.0 38.0 85-89 36.4103 38.0 38.0 38.0 33.8 38.0 90-94 34.22385 37.2 30.8 38.0 26.8 38.0 95-99 36.40895 38.0 37.8 38.0 33.8 38.0 100-104 36.15325 38.0 37.2 38.0 33.4 38.0 105-109 35.70205 38.0 36.2 38.0 31.2 38.0 110-114 35.30165 38.0 35.8 38.0 30.0 38.0 115-119 34.986599999999996 38.0 35.0 38.0 27.8 38.0 120-124 35.23695 38.0 35.0 38.0 30.0 38.0 125-129 34.7777 38.0 35.0 38.0 27.8 38.0 130-134 34.26285 38.0 34.4 38.0 24.6 38.0 135-139 33.80055 38.0 34.0 38.0 22.4 38.0 140-144 33.27955 38.0 34.0 38.0 20.0 38.0 145-149 32.099450000000004 37.2 32.8 38.0 11.4 38.0 150-151 27.725875000000002 34.5 17.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 0.0 13 0.0 14 1.0 15 1.0 16 1.0 17 1.0 18 3.0 19 12.0 20 4.0 21 7.0 22 10.0 23 6.0 24 15.0 25 10.0 26 14.0 27 23.0 28 48.0 29 40.0 30 59.0 31 86.0 32 107.0 33 192.0 34 359.0 35 578.0 36 1456.0 37 965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.07204838285564 6.757822771496187 7.0733631343676056 44.09676571128057 2 23.5 9.2 31.2 36.1 3 25.424999999999997 12.675 20.05 41.85 4 30.275000000000002 16.275000000000002 17.025000000000002 36.425000000000004 5 32.15 21.025 23.200000000000003 23.625 6 26.8 23.05 22.6 27.55 7 22.5 18.875 37.15 21.475 8 23.474999999999998 16.7 27.425 32.4 9 23.65 16.225 30.049999999999997 30.075000000000003 10-14 26.650000000000002 20.599999999999998 23.68 29.07 15-19 24.11 21.38 25.365 29.145 20-24 23.05 21.215 24.26 31.474999999999998 25-29 22.994999999999997 20.349999999999998 24.0 32.655 30-34 26.284999999999997 19.265 22.655 31.795 35-39 25.455 20.880000000000003 24.91 28.754999999999995 40-44 27.029999999999998 21.085 23.605 28.28 45-49 27.33 19.755 24.635 28.28 50-54 25.3 18.55 24.62 31.53 55-59 26.555 17.27 24.39 31.785000000000004 60-64 25.385 18.43 25.490000000000002 30.695 65-69 23.57 22.35 24.349999999999998 29.73 70-74 25.345000000000002 20.8 23.16 30.695 75-79 26.36 20.855 24.195 28.59 80-84 23.335 21.265 25.235000000000003 30.165 85-89 25.180000000000003 21.47 21.8 31.55 90-94 24.86 20.9 22.475 31.765 95-99 25.330000000000002 20.13 24.665 29.875 100-104 23.849999999999998 21.154999999999998 23.415 31.580000000000002 105-109 23.849999999999998 21.725 22.955000000000002 31.47 110-114 23.61 22.84 24.705 28.845 115-119 22.675 22.6 23.715 31.009999999999998 120-124 23.085 23.005 23.095 30.814999999999998 125-129 24.335 23.445 22.355 29.865000000000002 130-134 23.669999999999998 23.325000000000003 19.99 33.015 135-139 21.645 25.665 21.81 30.880000000000003 140-144 22.655 24.9 21.42 31.025000000000002 145-149 23.66 23.71 22.88 29.75 150-151 22.690336292036505 26.065758219777475 21.45268158519815 29.791223902987873 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.0 25 0.0 26 0.0 27 0.5 28 1.5 29 1.5 30 1.0 31 1.5 32 1.5 33 1.0 34 2.0 35 3.5 36 7.0 37 9.0 38 13.5 39 21.5 40 15.5 41 10.0 42 13.0 43 14.5 44 21.0 45 33.5 46 52.5 47 78.5 48 104.0 49 135.5 50 174.0 51 204.5 52 222.0 53 256.0 54 270.0 55 234.0 56 220.5 57 222.0 58 202.0 59 181.0 60 171.0 61 190.0 62 223.0 63 213.0 64 133.5 65 60.5 66 33.0 67 25.0 68 23.0 69 12.0 70 9.5 71 9.0 72 6.5 73 8.0 74 8.5 75 17.5 76 36.5 77 45.5 78 34.0 79 11.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.925 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.95 #Duplication Level Percentage of deduplicated Percentage of total 1 77.10592160133444 46.225 2 11.592994161801501 13.900000000000002 3 4.211843202668891 7.575 4 2.126772310258549 5.1 5 1.2093411175979984 3.6249999999999996 6 1.0425354462051708 3.75 7 0.5838198498748958 2.45 8 0.5004170141784821 2.4 9 0.3336113427856547 1.7999999999999998 >10 1.2510425354462051 11.675 >50 0.041701417848206836 1.5 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGC 60 1.5 No Hit GCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 36 0.8999999999999999 No Hit CCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT 30 0.75 No Hit GTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACAC 26 0.65 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 23 0.575 No Hit GTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGAC 23 0.575 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 22 0.5499999999999999 No Hit GTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGT 19 0.475 No Hit GCGCTATCTGCCGGGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGTG 19 0.475 No Hit CCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAG 17 0.42500000000000004 No Hit CGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATT 17 0.42500000000000004 No Hit GGCCGTTGCAGCGCAGTGCCCCGAGGGACACGCCTTGCGGCGCGCGGGTA 15 0.375 No Hit CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC 15 0.375 No Hit GCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGG 14 0.35000000000000003 No Hit GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC 13 0.325 No Hit CGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGG 13 0.325 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 12 0.3 No Hit GCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTT 12 0.3 No Hit CCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGT 12 0.3 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 12 0.3 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 11 0.27499999999999997 No Hit GTGCGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCC 11 0.27499999999999997 No Hit CCCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGGCT 11 0.27499999999999997 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 11 0.27499999999999997 No Hit CCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCG 11 0.27499999999999997 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 11 0.27499999999999997 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 11 0.27499999999999997 No Hit CACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTT 10 0.25 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 10 0.25 No Hit GCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGTTG 10 0.25 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 10 0.25 No Hit CCTTGTTCCGCTCTCGCTCGCCGCTACTAACGGGGTCTCGGTTGATTTTC 9 0.22499999999999998 No Hit GCGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAG 9 0.22499999999999998 No Hit GGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTG 9 0.22499999999999998 No Hit GGGTAATGACTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTG 9 0.22499999999999998 No Hit CCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCA 9 0.22499999999999998 No Hit GTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA 9 0.22499999999999998 No Hit GTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGC 9 0.22499999999999998 No Hit ATCCGTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCAC 9 0.22499999999999998 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 8 0.2 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 8 0.2 No Hit GTCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 8 0.2 No Hit CGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACACGC 8 0.2 No Hit GTAATGACTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTA 8 0.2 No Hit GGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACACGCC 8 0.2 No Hit CAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGA 8 0.2 No Hit GCCGTTGCAGCGCAGTGCCCCGAGGGACACGCCTTGCGGCGCGCGGGTAC 8 0.2 No Hit GTTCAGTAGTCAAAGCAACTTCGTCACTTTCGTGTACCCATCGGACGGCA 8 0.2 No Hit CGACAGCCGACGGGTTTGGGGCCGGGACCCCCGAGCCCAGTCCTCAGAGC 8 0.2 No Hit CTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGG 8 0.2 No Hit CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTC 8 0.2 No Hit CCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGT 7 0.17500000000000002 No Hit CGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGA 7 0.17500000000000002 No Hit GCAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG 7 0.17500000000000002 No Hit GGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCT 7 0.17500000000000002 No Hit GGCAGTTTAAAAGGTTGACCTATTTGGGAATCTCCGGATCTATGCTTATT 7 0.17500000000000002 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 7 0.17500000000000002 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 7 0.17500000000000002 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 7 0.17500000000000002 No Hit GGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGGCGTCTC 7 0.17500000000000002 No Hit CAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGT 7 0.17500000000000002 No Hit GGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACACCT 7 0.17500000000000002 No Hit CCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAA 7 0.17500000000000002 No Hit CGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTG 7 0.17500000000000002 No Hit GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATT 7 0.17500000000000002 No Hit GCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCA 6 0.15 No Hit GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 6 0.15 No Hit GCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCC 6 0.15 No Hit CCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCGTGCTCC 6 0.15 No Hit CATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAA 6 0.15 No Hit CCTGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGC 6 0.15 No Hit CCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGG 6 0.15 No Hit CTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCC 6 0.15 No Hit CCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAAGCGGTAGGAGC 6 0.15 No Hit CGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGAGCTGGAATT 6 0.15 No Hit GCTATCTGCCGGGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGTGCA 6 0.15 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 6 0.15 No Hit CCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGA 6 0.15 No Hit CGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACT 6 0.15 No Hit GCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGC 6 0.15 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 6 0.15 No Hit CCCGAGGAAACCTTTGCACGCCTCCGTTACCTTTTGGGAGGCCTACGCCC 6 0.15 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 6 0.15 No Hit GTTGGCAGGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCG 6 0.15 No Hit CCCGCCGGCGTCTCCGGACTTCCTAACGTCGCCGTCAACCGCCACATCCC 6 0.15 No Hit GTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGA 6 0.15 No Hit GTCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACT 6 0.15 No Hit GGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGA 6 0.15 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 6 0.15 No Hit GGTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACA 6 0.15 No Hit CTAGTATCCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTT 5 0.125 No Hit GGCCGACCCGGCCCAAAGTCCAACTACGAGCTTTTTAACTGCAACAACTT 5 0.125 No Hit CGCGCTATCTGCCGGGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGT 5 0.125 No Hit CCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCT 5 0.125 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 5 0.125 No Hit CCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGACACAATCTCGTAT 5 0.125 TruSeq Adapter, Index 7 (97% over 37bp) CACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCAGCGCAAGGGCGC 5 0.125 No Hit GTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCC 5 0.125 No Hit GTCAGGAATGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTG 5 0.125 No Hit GGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGGCAATTTCAAGCACT 5 0.125 No Hit CTCAGCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCA 5 0.125 No Hit GGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTT 5 0.125 No Hit CCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCGGGCCGGACGG 5 0.125 No Hit AGCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCT 5 0.125 No Hit CTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACC 5 0.125 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 5 0.125 No Hit CTCCCACCTATCCTACACATTCGATCAAGGTTGTCACTGCGAAGCTATAG 5 0.125 No Hit GCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGG 5 0.125 No Hit GGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGG 5 0.125 No Hit TGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGC 5 0.125 No Hit CCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCC 5 0.125 No Hit CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 5 0.125 No Hit CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC 5 0.125 No Hit CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATT 5 0.125 No Hit GTCCCGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCG 5 0.125 No Hit GTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTA 5 0.125 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 5 0.125 No Hit GCCCCCGATGCCTCTAATCATTGGCTTTACCTGATAGAACTCGTAATGGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1375 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.30000000000000004 0.0 0.0 0.0 0.0 68-69 0.4375 0.0 0.0 0.0 0.0 70-71 0.6 0.0 0.0 0.0 0.0 72-73 0.75 0.0 0.0 0.0 0.0 74-75 0.9375 0.0 0.0 0.0 0.0 76-77 1.175 0.0 0.0 0.0 0.0 78-79 1.4125 0.0 0.0 0.0 0.0 80-81 1.75 0.0 0.0 0.0 0.0 82-83 2.2 0.0 0.0 0.0 0.0 84-85 2.6 0.0 0.0 0.0 0.0 86-87 2.9 0.0 0.0 0.0 0.0 88-89 3.275 0.0 0.0 0.0 0.0 90-91 3.7375 0.0 0.0 0.0 0.0 92-93 4.35 0.0 0.0 0.0 0.0 94-95 5.0 0.0 0.0 0.0 0.0 96-97 5.7 0.0 0.0 0.0 0.0 98-99 6.3625 0.0 0.0 0.0 0.0 100-101 7.15 0.0 0.0 0.0 0.0 102-103 7.925000000000001 0.0 0.0 0.0 0.0 104-105 8.825 0.0 0.0 0.0 0.0 106-107 9.675 0.0 0.0 0.0 0.0 108-109 10.6375 0.0 0.0 0.0 0.0 110-111 11.3375 0.0 0.0 0.0 0.0 112-113 12.3 0.0 0.0 0.0 0.0 114-115 13.4625 0.0 0.0 0.0 0.0 116-117 14.7 0.0 0.0 0.0 0.0 118-119 16.2 0.0 0.0 0.0 0.0 120-121 17.75 0.0 0.0 0.0 0.0 122-123 19.1375 0.0 0.0 0.0 0.0 124-125 20.475 0.0 0.0 0.0 0.0 126-127 22.05 0.0 0.0 0.0 0.0 128-129 23.65 0.0 0.0 0.0 0.0 130-131 24.8 0.0 0.0 0.0 0.0 132-133 25.825 0.0 0.0 0.0 0.0 134-135 27.112499999999997 0.0 0.0 0.0 0.0 136-137 28.5 0.0 0.0 0.0 0.0 138-139 29.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGGAT 10 0.0060887975 150.61038 1 TCGGATG 10 0.006836113 144.9625 2 TTGAAAA 25 8.7222434E-4 86.97751 145 GTATGCC 20 0.005942617 28.992498 125-129 CCGTCTT 20 0.005942617 28.992498 130-134 GGGGGCA 20 0.005942617 28.992498 90-94 CGTATGC 20 0.005942617 28.992498 125-129 GCTTGAA 20 0.005942617 28.992498 140-144 GCCACCG 20 0.005942617 28.992498 85-89 GCCGTCT 20 0.005942617 28.992498 130-134 CTTCTGC 20 0.005942617 28.992498 135-139 AACACTT 35 1.1987623E-4 24.850716 15-19 GGTAGGA 30 0.0014459731 24.160418 40-44 CTTCACC 35 0.0035419178 20.70893 20-24 TTGCAAT 35 0.0035419178 20.70893 135-139 CCGGACC 35 0.0035419178 20.70893 25-29 ATGATCT 35 0.0035419178 20.70893 140-144 GGACCAT 35 0.0035419178 20.70893 25-29 ACACTTC 35 0.0035419178 20.70893 15-19 GATCCGA 35 0.0035419178 20.70893 9 >>END_MODULE SRR6257528 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6257528_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7995 33.0 33.0 34.0 32.0 34.0 2 32.8035 34.0 33.0 34.0 32.0 34.0 3 32.8855 34.0 33.0 34.0 32.0 34.0 4 33.006 34.0 33.0 34.0 32.0 34.0 5 32.892 34.0 33.0 34.0 32.0 34.0 6 37.14525 38.0 38.0 38.0 36.0 38.0 7 33.13725 38.0 31.0 38.0 16.0 38.0 8 35.986 38.0 36.0 38.0 30.0 38.0 9 35.9855 38.0 37.0 38.0 31.0 38.0 10-14 36.971199999999996 38.0 38.0 38.0 35.8 38.0 15-19 36.8993 38.0 38.0 38.0 35.6 38.0 20-24 37.183299999999996 38.0 38.0 38.0 36.8 38.0 25-29 37.21704999999999 38.0 38.0 38.0 37.0 38.0 30-34 37.146 38.0 38.0 38.0 36.8 38.0 35-39 37.11235 38.0 38.0 38.0 36.4 38.0 40-44 35.8511 38.0 35.8 38.0 30.8 38.0 45-49 36.70975 38.0 37.8 38.0 35.0 38.0 50-54 36.69345 38.0 38.0 38.0 35.2 38.0 55-59 36.59685 38.0 38.0 38.0 34.4 38.0 60-64 36.781000000000006 38.0 38.0 38.0 35.4 38.0 65-69 36.654250000000005 38.0 38.0 38.0 35.0 38.0 70-74 36.5421 38.0 38.0 38.0 34.6 38.0 75-79 35.101749999999996 37.8 35.0 38.0 28.6 38.0 80-84 35.889199999999995 38.0 37.0 38.0 29.8 38.0 85-89 35.05630000000001 38.0 35.4 38.0 27.6 38.0 90-94 35.739050000000006 38.0 37.0 38.0 31.6 38.0 95-99 32.6381 35.2 29.2 38.0 24.8 38.0 100-104 35.76585 38.0 36.2 38.0 32.4 38.0 105-109 36.007099999999994 38.0 37.8 38.0 33.0 38.0 110-114 35.68044999999999 38.0 36.2 38.0 32.8 38.0 115-119 34.017450000000004 37.4 32.4 38.0 25.8 38.0 120-124 34.138400000000004 37.6 34.0 38.0 25.6 38.0 125-129 29.32455 33.0 22.2 37.0 15.8 38.0 130-134 28.09635 30.8 21.2 36.4 13.0 38.0 135-139 32.6031 37.4 32.8 38.0 16.4 38.0 140-144 28.102599999999995 31.2 24.2 36.4 8.6 38.0 145-149 28.138150000000003 33.2 25.0 37.4 2.0 38.0 150-151 24.614375 31.5 14.0 36.5 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 4.0 5 0.0 6 5.0 7 1.0 8 2.0 9 2.0 10 1.0 11 2.0 12 2.0 13 0.0 14 0.0 15 5.0 16 0.0 17 5.0 18 4.0 19 6.0 20 8.0 21 10.0 22 14.0 23 14.0 24 24.0 25 34.0 26 31.0 27 38.0 28 46.0 29 52.0 30 68.0 31 116.0 32 146.0 33 270.0 34 451.0 35 821.0 36 1341.0 37 470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.025 13.700000000000001 7.35 33.925 2 37.925 15.85 20.325 25.900000000000002 3 30.7 23.599999999999998 20.349999999999998 25.35 4 31.075000000000003 30.425 17.1 21.4 5 31.025000000000002 29.549999999999997 17.224999999999998 22.2 6 30.175 36.0 14.274999999999999 19.55 7 27.675 18.275 33.825 20.225 8 31.05 18.775 23.025000000000002 27.150000000000002 9 29.175 21.349999999999998 24.3 25.174999999999997 10-14 29.294999999999998 25.27 21.855 23.580000000000002 15-19 29.9 23.880000000000003 24.18 22.040000000000003 20-24 30.61 23.465 22.335 23.59 25-29 29.34 24.404999999999998 21.23 25.025 30-34 32.653265326532654 23.407340734073408 20.362036203620363 23.577357735773578 35-39 34.455000000000005 24.445 19.925 21.175 40-44 34.58845884588459 23.497349734973497 20.7020702070207 21.21212121212121 45-49 32.505 24.834999999999997 19.36 23.3 50-54 30.37 24.465 19.24 25.924999999999997 55-59 29.909999999999997 24.89 22.689999999999998 22.509999999999998 60-64 33.1 23.76 21.09 22.05 65-69 32.365 23.05 19.255 25.330000000000002 70-74 33.155 25.455 16.7 24.69 75-79 31.259999999999998 25.66 19.185 23.895 80-84 33.39666983349168 23.12615630781539 18.605930296514824 24.87124356217811 85-89 31.16 23.69 19.400000000000002 25.75 90-94 32.875 24.665 18.8 23.66 95-99 30.964999999999996 24.9 21.255 22.88 100-104 30.464999999999996 24.865000000000002 21.595 23.075000000000003 105-109 29.78 22.67 22.869999999999997 24.68 110-114 29.880000000000003 26.0 19.66 24.46 115-119 31.65 23.11 19.919999999999998 25.319999999999997 120-124 29.925 25.355 19.885 24.834999999999997 125-129 30.34 26.045 20.265 23.35 130-134 32.17 25.47 19.220000000000002 23.14 135-139 32.17 23.955000000000002 19.465 24.41 140-144 33.2 24.224999999999998 19.045 23.53 145-149 32.29 26.85 19.185 21.675 150-151 31.6875 27.150000000000002 22.225 18.9375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.0 24 0.5 25 0.5 26 0.0 27 0.0 28 0.5 29 0.5 30 0.0 31 0.0 32 0.0 33 0.0 34 1.5 35 3.0 36 3.5 37 3.0 38 3.5 39 7.0 40 11.5 41 18.5 42 22.0 43 25.0 44 31.5 45 45.0 46 74.5 47 135.5 48 177.5 49 190.5 50 195.0 51 220.5 52 246.5 53 238.5 54 265.0 55 259.0 56 214.0 57 177.0 58 149.5 59 132.5 60 123.5 61 143.0 62 148.5 63 125.0 64 86.0 65 50.5 66 48.0 67 63.0 68 58.0 69 39.5 70 34.5 71 29.0 72 16.0 73 12.0 74 17.5 75 37.5 76 56.0 77 39.5 78 14.5 79 4.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.01 35-39 0.0 40-44 0.01 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.005 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.574999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92307692307693 42.75 2 11.740890688259109 13.05 3 4.228520017993702 7.049999999999999 4 2.06927575348628 4.6 5 1.3045434098065678 3.6249999999999996 6 0.8097165991902834 2.7 7 0.5847953216374269 2.275 8 0.2699055330634278 1.2 9 0.1349527665317139 0.675 >10 1.8893387314439947 20.3 >50 0.0449842555105713 1.775 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGAC 71 1.775 No Hit GCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCC 48 1.2 No Hit CCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATC 44 1.0999999999999999 No Hit GCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGC 37 0.9249999999999999 No Hit GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC 35 0.8750000000000001 No Hit GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGT 28 0.7000000000000001 No Hit AGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGA 28 0.7000000000000001 No Hit GGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAG 27 0.675 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 27 0.675 No Hit CATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCT 25 0.625 No Hit CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAAC 25 0.625 No Hit CGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGC 24 0.6 No Hit GGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCA 23 0.575 No Hit GCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATA 21 0.525 No Hit CGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCA 21 0.525 No Hit CGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACG 21 0.525 No Hit GGACTGTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCC 20 0.5 No Hit CCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAAT 19 0.475 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 19 0.475 No Hit GGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAA 19 0.475 No Hit CCCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGA 18 0.44999999999999996 No Hit CTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCA 18 0.44999999999999996 No Hit GCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAA 17 0.42500000000000004 No Hit GGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAA 17 0.42500000000000004 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 16 0.4 No Hit CTGTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCCCCC 15 0.375 No Hit CGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATG 14 0.35000000000000003 No Hit GGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGT 13 0.325 No Hit GTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCGCCTCG 13 0.325 No Hit GTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTGCCCCCGG 13 0.325 No Hit GGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTA 13 0.325 No Hit AGGGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGC 12 0.3 No Hit ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAA 12 0.3 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 12 0.3 No Hit CGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGG 12 0.3 No Hit GGCCGACGAGCCGCGTCCGGCCGCCTCGAAGCTCCCTTCCCCACGGGCGG 11 0.27499999999999997 No Hit CAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTG 11 0.27499999999999997 No Hit GACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCAT 11 0.27499999999999997 No Hit ACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATT 11 0.27499999999999997 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 11 0.27499999999999997 No Hit GCGGGACGTTCTGTCAATCGGGGAAGGTTTTTGGTGACAAGACCTGGAGA 11 0.27499999999999997 No Hit TACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGA 10 0.25 No Hit GGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAG 10 0.25 No Hit CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCTACCATGG 9 0.22499999999999998 No Hit GTGGACTGTTGTCGGCCGTGCTGGCGGCCCAAGCCCGGGGTCCTTAGGTG 9 0.22499999999999998 No Hit GGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCAT 9 0.22499999999999998 No Hit GGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAAC 8 0.2 No Hit GGGGCTTTCCCCGAGCGCTGAACAGTCGACTCAGAACTGGTACGGACAAG 8 0.2 No Hit GGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCG 8 0.2 No Hit CCGAAATGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGAC 8 0.2 No Hit CCGACATGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATC 8 0.2 No Hit CTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTT 8 0.2 No Hit CCGCTCGGCTCGGGGCGTGGACTGTTGTCGGCCGTGCTGGCGGCCCAAGC 7 0.17500000000000002 No Hit GCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTG 7 0.17500000000000002 No Hit CATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGG 7 0.17500000000000002 No Hit GGCCCGTGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCA 7 0.17500000000000002 No Hit ATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGC 7 0.17500000000000002 No Hit CTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTCCGGTGAGCCGCGCC 7 0.17500000000000002 No Hit GCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTG 7 0.17500000000000002 No Hit GACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGG 7 0.17500000000000002 No Hit CGTGCCTCCGGCATCGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGC 7 0.17500000000000002 No Hit GTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTC 7 0.17500000000000002 No Hit CATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAACAA 7 0.17500000000000002 No Hit TGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACGGA 7 0.17500000000000002 No Hit ACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGG 7 0.17500000000000002 No Hit GTAGCGGGACGTTCTGTCAATCGGGGAAGGTTTTTGGTGACAAGACCTGG 6 0.15 No Hit CGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCC 6 0.15 No Hit CCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATT 6 0.15 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 6 0.15 No Hit TTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCTACCAT 6 0.15 No Hit CCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTCCGGTGAGC 6 0.15 No Hit GCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAA 6 0.15 No Hit AGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAAT 6 0.15 No Hit GGGAGCGTTCCGCCTTAGAGGGAAGCAACCGCGAAAGCGGGGGTCGACGA 6 0.15 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 6 0.15 No Hit GCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTT 6 0.15 No Hit GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT 6 0.15 No Hit ACCGACTGATGTTGAAGAATCAGCGGATGAGTTGTGGTTAGGGGTGAAAT 6 0.15 No Hit GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAA 6 0.15 No Hit GTTGAAGAATCAGCGGATGAGTTGTGGTTAGGGGTGAAATGCCACTCGAA 6 0.15 No Hit AAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCG 6 0.15 No Hit CCGACTGATGTTGAAGAATCAGCGGATGAGTTGTGGTTAGGGGTGAAATG 6 0.15 No Hit GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGAT 6 0.15 No Hit AAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGC 5 0.125 No Hit CGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGG 5 0.125 No Hit GGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGGCGACACACGTGCT 5 0.125 No Hit ATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAGT 5 0.125 No Hit GTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAATAGGGACAGT 5 0.125 No Hit GGAGCGTTCCGCCTTAGAGGGAAGCAACCGCGAAAGCGGGGGTCGACGAA 5 0.125 No Hit CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA 5 0.125 No Hit GAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACGT 5 0.125 No Hit AATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGT 5 0.125 No Hit CTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAA 5 0.125 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 5 0.125 No Hit GCCTCCGGCATCGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCAT 5 0.125 No Hit CGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACGTTAGG 5 0.125 No Hit CGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTG 5 0.125 No Hit GGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCTCGCGG 5 0.125 No Hit ATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACGTTA 5 0.125 No Hit ATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCA 5 0.125 No Hit GTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAG 5 0.125 No Hit CCCCCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTT 5 0.125 No Hit GGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCC 5 0.125 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGACACAGTGTAGATCT 5 0.125 Illumina Single End PCR Primer 1 (96% over 32bp) CCGAAACCGATCGATCTAGCCATGAGCAGGTTGAAGAGAGCTCTAACAGG 5 0.125 No Hit CGACAGTCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCA 5 0.125 No Hit TAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAA 5 0.125 No Hit ATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGG 5 0.125 No Hit GTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGT 5 0.125 No Hit GCTAGGCGAAGCGGTTGAGTGCCGCACCCTAGATGGCTAAAGTCCAGTAG 5 0.125 No Hit GACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCAT 5 0.125 No Hit CCCCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.16249999999999998 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.35 0.0 0.0 0.0 0.0 68-69 0.45 0.0 0.0 0.0 0.0 70-71 0.6 0.0 0.0 0.0 0.0 72-73 0.725 0.0 0.0 0.0 0.0 74-75 0.9125 0.0 0.0 0.0 0.0 76-77 1.1625 0.0 0.0 0.0 0.0 78-79 1.4125 0.0 0.0 0.0 0.0 80-81 1.8125 0.0 0.0 0.0 0.0 82-83 2.325 0.0 0.0 0.0 0.0 84-85 2.7125000000000004 0.0 0.0 0.0 0.0 86-87 2.9375 0.0 0.0 0.0 0.0 88-89 3.2875 0.0 0.0 0.0 0.0 90-91 3.6875 0.0 0.0 0.0 0.0 92-93 4.15 0.0 0.0 0.0 0.0 94-95 4.675000000000001 0.0 0.0 0.0 0.0 96-97 5.300000000000001 0.0 0.0 0.0 0.0 98-99 5.975 0.0 0.0 0.0 0.0 100-101 6.775 0.0 0.0 0.0 0.0 102-103 7.5625 0.0 0.0 0.0 0.0 104-105 8.5 0.0 0.0 0.0 0.0 106-107 9.399999999999999 0.0 0.0 0.0 0.0 108-109 10.3125 0.0 0.0 0.0 0.0 110-111 10.95 0.0 0.0 0.0 0.0 112-113 11.7625 0.0 0.0 0.0 0.0 114-115 12.8 0.0 0.0 0.0 0.0 116-117 13.6 0.0 0.0 0.0 0.0 118-119 14.55 0.0 0.0 0.0 0.0 120-121 15.3625 0.0 0.0 0.0 0.0 122-123 16.0 0.0 0.0 0.0 0.0 124-125 16.75 0.0 0.0 0.0 0.0 126-127 17.575 0.0 0.0 0.0 0.0 128-129 18.7125 0.0 0.0 0.0 0.0 130-131 19.575000000000003 0.0 0.0 0.0 0.0 132-133 20.3125 0.0 0.0 0.0 0.0 134-135 21.2625 0.0 0.0 0.0 0.0 136-137 22.2375 0.0 0.0 0.0 0.0 138-139 23.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTGTC 15 1.1411342E-4 145.0 5 ACTGTTG 15 1.1411342E-4 145.0 3 GGACTGT 15 1.1411342E-4 145.0 1 TATCATT 15 1.1411342E-4 145.0 145 CTGTTGT 15 1.1411342E-4 145.0 4 GTTGTCG 15 1.1411342E-4 145.0 6 ATGAGAA 10 0.006830828 145.0 4 GACTGTT 20 3.5877043E-4 108.75 2 TTGTCGG 25 8.7132835E-4 87.0 7 GTCGGCC 25 8.7132835E-4 87.0 9 TGTCGGC 30 0.0017973486 72.5 8 >>END_MODULE Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732941 spots for SRR6257528.sra Written 732941 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra Read 732931 spots for SRR6257528.sra Written 732931 spots for SRR6257528.sra SRR ids: ['SRR6257528.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_kdqyk2vn SRR6257528.sra spots: 14658630 blocks: [[1, 732931], [732932, 1465862], [1465863, 2198793], [2198794, 2931724], [2931725, 3664655], [3664656, 4397586], [4397587, 5130517], [5130518, 5863448], [5863449, 6596379], [6596380, 7329310], [7329311, 8062241], [8062242, 8795172], [8795173, 9528103], [9528104, 10261034], [10261035, 10993965], [10993966, 11726896], [11726897, 12459827], [12459828, 13192758], [13192759, 13925689], [13925690, 14658630]] SRR6257528 file size 4945628 SRR6257528 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6257528 SRR6257528_1.fastq SRR6257528_2.fastq Input file: SRR6257528_1.fastq Paired file: SRR6257528_2.fastq trimmed: SRR6257528-trimmed-pair1.fastq, SRR6257528-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 09:06:18 2024 >> started Sat Dec 7 09:06:35 2024 >> done (17.113s) 14658630 read pairs processed; of these: 10799 ( 0.07%) short read pairs filtered out after trimming by size control 49153 ( 0.34%) empty read pairs filtered out after trimming by size control 14598678 (99.59%) read pairs available; of these: 10077680 (69.03%) trimmed read pairs available after processing 4520998 (30.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 16 0.00% 20 9 0.00% 21 19 0.00% 22 28 0.00% 23 25 0.00% 24 42 0.00% 25 67 0.00% 26 102 0.00% 27 90 0.00% 28 117 0.00% 29 159 0.00% 30 156 0.00% 31 160 0.00% 32 179 0.00% 33 195 0.00% 34 237 0.00% 35 214 0.00% 36 275 0.00% 37 305 0.00% 38 353 0.00% 39 480 0.00% 40 485 0.00% 41 548 0.00% 42 618 0.00% 43 559 0.00% 44 567 0.00% 45 635 0.00% 46 698 0.00% 47 822 0.01% 48 1129 0.01% 49 1339 0.01% 50 1489 0.01% 51 1669 0.01% 52 1661 0.01% 53 1710 0.01% 54 1794 0.01% 55 1748 0.01% 56 1995 0.01% 57 2148 0.01% 58 2474 0.02% 59 2727 0.02% 60 3068 0.02% 61 4454 0.03% 62 4790 0.03% 63 5913 0.04% 64 6004 0.04% 65 6023 0.04% 66 7127 0.05% 67 9145 0.06% 68 10117 0.07% 69 10173 0.07% 70 11690 0.08% 71 13168 0.09% 72 14921 0.10% 73 15187 0.10% 74 16214 0.11% 75 16370 0.11% 76 17099 0.12% 77 17669 0.12% 78 18608 0.13% 79 20687 0.14% 80 22397 0.15% 81 25065 0.17% 82 29987 0.21% 83 32446 0.22% 84 31236 0.21% 85 30444 0.21% 86 29900 0.20% 87 31100 0.21% 88 30240 0.21% 89 31086 0.21% 90 30957 0.21% 91 36035 0.25% 92 43111 0.30% 93 43803 0.30% 94 50071 0.34% 95 51356 0.35% 96 59808 0.41% 97 59475 0.41% 98 75698 0.52% 99 70518 0.48% 100 75883 0.52% 101 72098 0.49% 102 74207 0.51% 103 76808 0.53% 104 77618 0.53% 105 69671 0.48% 106 73301 0.50% 107 78452 0.54% 108 65885 0.45% 109 70478 0.48% 110 75744 0.52% 111 80437 0.55% 112 107032 0.73% 113 87141 0.60% 114 93806 0.64% 115 102157 0.70% 116 108458 0.74% 117 95883 0.66% 118 103482 0.71% 119 104982 0.72% 120 108279 0.74% 121 102204 0.70% 122 129041 0.88% 123 118865 0.81% 124 117785 0.81% 125 113210 0.78% 126 125503 0.86% 127 135150 0.93% 128 103221 0.71% 129 118480 0.81% 130 112572 0.77% 131 111604 0.76% 132 111927 0.77% 133 114822 0.79% 134 101359 0.69% 135 103646 0.71% 136 102529 0.70% 137 111654 0.76% 138 112966 0.77% 139 120837 0.83% 140 127736 0.87% 141 148999 1.02% 142 152253 1.04% 143 166613 1.14% 144 183518 1.26% 145 196436 1.35% 146 237768 1.63% 147 282426 1.93% 148 381836 2.62% 149 686423 4.70% 150 2471288 16.93% 151 4520998 30.97% 14598678 reads passed initial QC criterion=sequence-density sequence-density=6.41 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=31 prefix-density=6.43 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=30.71 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=1.0 sequence=ACGACACGACCCTTGGTGGGGGAGGGGAGGGACGAATCCGTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGCCACTTACAATGCCCCGTCGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAGGCGGCCGGACGCGGCTCGTCGGCCGGAACGGCTTAGCCAATGGCACGTGCCCTTGGGGGCGCAAGCGCCCCTAACGTGGGTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGACTTAGAGGCGTTCAGTCATAATCCGGCACACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGTGAATCAACGGTTCCTCTCGTAC criterion=sequence-density sequence-density=4.81 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=28 prefix-density=4.90 prefix-fanout=1.9 sequence=CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGGCGATGC criterion=fanout-score sequence-density=0.34 sequence-density-rank=19 fanout-score=27.42 fanout-score-rank=1 prefix-density=4.16 prefix-fanout=2.2 sequence=GACCGATAGCGTACAAGTACCGTGAG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGGCGATGC -o SRR6257528 SRR6257528_1.fastq SRR6257528_2.fastq Input file: SRR6257528_1.fastq Paired file: SRR6257528_2.fastq trimmed: SRR6257528-trimmed-pair1.fastq, SRR6257528-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 09:08:06 2024 >> started Sat Dec 7 09:08:17 2024 >> done (10.844s) 9732452 read pairs processed; of these: 523 ( 0.01%) short read pairs filtered out after trimming by size control 619 ( 0.01%) empty read pairs filtered out after trimming by size control 9731310 (99.99%) read pairs available; of these: 7155 ( 0.07%) trimmed read pairs available after processing 9724155 (99.93%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 13 0.00% 20 8 0.00% 21 9 0.00% 22 21 0.00% 23 18 0.00% 24 27 0.00% 25 43 0.00% 26 76 0.00% 27 57 0.00% 28 80 0.00% 29 99 0.00% 30 106 0.00% 31 110 0.00% 32 123 0.00% 33 130 0.00% 34 165 0.00% 35 137 0.00% 36 193 0.00% 37 199 0.00% 38 243 0.00% 39 331 0.00% 40 320 0.00% 41 360 0.00% 42 411 0.00% 43 387 0.00% 44 365 0.00% 45 410 0.00% 46 454 0.00% 47 546 0.01% 48 763 0.01% 49 880 0.01% 50 1005 0.01% 51 1107 0.01% 52 1125 0.01% 53 1158 0.01% 54 1224 0.01% 55 1147 0.01% 56 1298 0.01% 57 1386 0.01% 58 1632 0.02% 59 1826 0.02% 60 2017 0.02% 61 2949 0.03% 62 3222 0.03% 63 3930 0.04% 64 4057 0.04% 65 4020 0.04% 66 4781 0.05% 67 6111 0.06% 68 6706 0.07% 69 6827 0.07% 70 7771 0.08% 71 8870 0.09% 72 9969 0.10% 73 10113 0.10% 74 10819 0.11% 75 10964 0.11% 76 11329 0.12% 77 11815 0.12% 78 12426 0.13% 79 13637 0.14% 80 14939 0.15% 81 16601 0.17% 82 20041 0.21% 83 21556 0.22% 84 20866 0.21% 85 20278 0.21% 86 20031 0.21% 87 20604 0.21% 88 20153 0.21% 89 20747 0.21% 90 20613 0.21% 91 23960 0.25% 92 28643 0.29% 93 29095 0.30% 94 33448 0.34% 95 34396 0.35% 96 39968 0.41% 97 39566 0.41% 98 50451 0.52% 99 46944 0.48% 100 50506 0.52% 101 48101 0.49% 102 49297 0.51% 103 51209 0.53% 104 51638 0.53% 105 46513 0.48% 106 48817 0.50% 107 52290 0.54% 108 43945 0.45% 109 46918 0.48% 110 50431 0.52% 111 53724 0.55% 112 71421 0.73% 113 58172 0.60% 114 62727 0.64% 115 68001 0.70% 116 72216 0.74% 117 63956 0.66% 118 68844 0.71% 119 69877 0.72% 120 72173 0.74% 121 68305 0.70% 122 85950 0.88% 123 79294 0.81% 124 78566 0.81% 125 75531 0.78% 126 83676 0.86% 127 89978 0.92% 128 68708 0.71% 129 79090 0.81% 130 75125 0.77% 131 74209 0.76% 132 74393 0.76% 133 76672 0.79% 134 67427 0.69% 135 68853 0.71% 136 68349 0.70% 137 74304 0.76% 138 75106 0.77% 139 80735 0.83% 140 85023 0.87% 141 99404 1.02% 142 101435 1.04% 143 111104 1.14% 144 122396 1.26% 145 131531 1.35% 146 158795 1.63% 147 188438 1.94% 148 254640 2.62% 149 457235 4.70% 150 1646615 16.92% 151 3014419 30.98% criterion=sequence-density sequence-density=6.40 sequence-density-rank=1 fanout-score=1.94 fanout-score-rank=31 prefix-density=6.44 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.40 sequence-density-rank=20 fanout-score=27.78 fanout-score-rank=1 prefix-density=11.16 prefix-fanout=1.0 sequence=CGGTGTGTACAGGGCCCGGGTACATATTCACCGCGGCATGCTGATCCGCGATTACTAG criterion=sequence-density sequence-density=4.78 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=28 prefix-density=4.86 prefix-fanout=2.0 sequence=CCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCACATTAGTGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGGCGATGC criterion=fanout-score sequence-density=0.33 sequence-density-rank=17 fanout-score=27.93 fanout-score-rank=1 prefix-density=4.14 prefix-fanout=2.2 sequence=GACCGATAGCGTACAAGTACCGTGAG SRR6257528 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 09:09:02 Started mapping on | Dec 07 09:09:02 Finished on | Dec 07 09:12:52 Mapping speed, Million of reads per hour | 228.48 Number of input reads | 14597536 Average input read length | 274 UNIQUE READS: Uniquely mapped reads number | 1997658 Uniquely mapped reads % | 13.68% Average mapped length | 281.55 Number of splices: Total | 113081 Number of splices: Annotated (sjdb) | 95846 Number of splices: GT/AG | 103815 Number of splices: GC/AG | 1733 Number of splices: AT/AC | 136 Number of splices: Non-canonical | 7397 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.06% Deletion average length | 2.96 Insertion rate per base | 0.00% Insertion average length | 1.60 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2228187 % of reads mapped to multiple loci | 15.26% Number of reads mapped to too many loci | 2551429 % of reads mapped to too many loci | 17.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.68% % of reads unmapped: other | 45.90% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10373022 10373022 10373022 N_multimapping 2228187 2228187 2228187 N_noFeature 1561344 1966719 1588071 N_ambiguous 57834 250 53770 UnstrandedReadsAssigned:378480 PositiveStrandReadsAssigned:30689 NegativeStrandReadsAssigned:355817 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=121 echo kmer=117 SRR6257528 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6257528-trimmed-pair1.fastq SRR6257528-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,597,536 reads, 731,669 reads pseudoaligned [quant] estimated average fragment length: 175.627 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 753 rounds 52973 SRR6257528.ke.tsv 35125 SRR6257528.se.tsv 88098 total ==> SRR6257528.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 761.434 4 4.13349 PNS24247 1044 869.373 0 0 PNS24249 1928 1753.37 0 0 PNS24246 1044 869.373 0 0 PNS24248 1044 869.373 0 0 PNS24244 1471 1296.37 0 0 PNS24243 293 123.557 0 0 KQK14069 1603 1428.37 18.985 10.4582 KQK14071 474 299.818 11.015 28.9079 ==> SRR6257528.se.tsv <== BRADI_1g14170v3 29 BRADI_1g53295v3 4 BRADI_1g59795v3 3 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 5 BRADI_1g74790v3 5 BRADI_1g09890v3 0 BRADI_1g77505v3 1 BRADI_1g48960v3 0 SRR6257528 completed mapping pipeline successfully