Starting /dee2/code/volunteer_pipeline.sh SRR6257529 current disk space = 1544252076032 free memory = 1604276188 SRR6257529 SRAfilesize 285fa23688c9657598e03fc1334b77c0 SRR6257529.sra SRR6257529.sra file validated SRR6257529 is paired end SRR6257529 is conventional basespace SRR6257529 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6257529_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.24325 25.0 18.0 32.0 18.0 33.0 2 27.4215 29.0 25.0 31.0 18.0 33.0 3 30.66525 31.0 29.0 33.0 27.0 33.0 4 31.062 33.0 31.0 33.0 28.0 33.0 5 31.5845 33.0 31.0 33.0 29.0 33.0 6 35.86275 37.0 36.0 38.0 33.0 38.0 7 35.13075 38.0 36.0 38.0 29.0 38.0 8 36.9505 38.0 37.0 38.0 35.0 38.0 9 37.1675 38.0 38.0 38.0 36.0 38.0 10-14 37.45545 38.0 38.0 38.0 37.0 38.0 15-19 37.55685 38.0 38.0 38.0 37.8 38.0 20-24 37.455 38.0 38.0 38.0 37.2 38.0 25-29 36.81675 38.0 37.8 38.0 34.6 38.0 30-34 37.38415 38.0 38.0 38.0 37.0 38.0 35-39 37.44825 38.0 38.0 38.0 37.2 38.0 40-44 36.396 38.0 37.0 38.0 31.6 38.0 45-49 36.907250000000005 38.0 37.8 38.0 35.2 38.0 50-54 37.16485 38.0 38.0 38.0 36.4 38.0 55-59 37.2266 38.0 38.0 38.0 36.2 38.0 60-64 37.1312 38.0 38.0 38.0 35.8 38.0 65-69 37.1659 38.0 38.0 38.0 36.0 38.0 70-74 36.227999999999994 38.0 36.8 38.0 30.8 38.0 75-79 35.4291 37.8 35.4 38.0 29.8 38.0 80-84 35.7673 38.0 36.4 38.0 31.0 38.0 85-89 35.343199999999996 37.8 35.2 38.0 28.8 38.0 90-94 36.37455 38.0 37.2 38.0 33.4 38.0 95-99 36.55925 38.0 38.0 38.0 34.0 38.0 100-104 36.41315 38.0 37.8 38.0 33.8 38.0 105-109 34.9901 38.0 35.4 38.0 26.2 38.0 110-114 35.78865 38.0 36.0 38.0 31.4 38.0 115-119 35.36815 38.0 35.4 38.0 29.4 38.0 120-124 35.346500000000006 38.0 35.4 38.0 30.2 38.0 125-129 34.981500000000004 38.0 35.4 38.0 28.6 38.0 130-134 34.561099999999996 38.0 34.6 38.0 26.4 38.0 135-139 31.54895 35.4 27.8 38.0 18.0 38.0 140-144 33.41645 38.0 33.6 38.0 21.8 38.0 145-149 32.81485 38.0 34.0 38.0 15.6 38.0 150-151 28.489 36.0 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 4.0 19 1.0 20 6.0 21 6.0 22 1.0 23 9.0 24 8.0 25 15.0 26 19.0 27 21.0 28 36.0 29 41.0 30 55.0 31 75.0 32 96.0 33 198.0 34 290.0 35 581.0 36 1366.0 37 1169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.561310782241016 10.174418604651162 5.89323467230444 44.37103594080338 2 20.674999999999997 11.95 34.2 33.175 3 19.900000000000002 15.825 26.5 37.775 4 24.25 23.150000000000002 22.775000000000002 29.825000000000003 5 24.675 28.625 23.549999999999997 23.150000000000002 6 20.974999999999998 31.474999999999998 24.224999999999998 23.325000000000003 7 16.35 23.95 40.75 18.95 8 19.5 21.275 31.825 27.400000000000002 9 19.400000000000002 20.599999999999998 36.125 23.875 10-14 21.485000000000003 26.290000000000003 25.419999999999998 26.805 15-19 22.61 24.95 26.27 26.169999999999998 20-24 21.62 26.174999999999997 26.33 25.874999999999996 25-29 23.23 24.4 26.525 25.845000000000002 30-34 22.625 25.165 26.295 25.915 35-39 21.245 24.905 27.865000000000002 25.985000000000003 40-44 21.89 25.224999999999998 26.71 26.174999999999997 45-49 21.085 24.285 27.665 26.965 50-54 21.65 24.825 26.55 26.974999999999998 55-59 21.37 24.98 26.68 26.97 60-64 22.62 24.235 27.060000000000002 26.085 65-69 22.145 24.66 26.745 26.450000000000003 70-74 23.325000000000003 25.11 25.045 26.52 75-79 21.865000000000002 24.855 26.685 26.595000000000002 80-84 22.34 26.825 25.415 25.419999999999998 85-89 22.62 24.865000000000002 26.39 26.125 90-94 21.83 25.405 26.174999999999997 26.590000000000003 95-99 22.595000000000002 25.759999999999998 25.345000000000002 26.3 100-104 21.755 25.995 26.21 26.040000000000003 105-109 21.98 25.285000000000004 25.95 26.784999999999997 110-114 22.255 25.924999999999997 26.395000000000003 25.424999999999997 115-119 21.925 25.900000000000002 25.795 26.38 120-124 22.15 27.04 23.880000000000003 26.93 125-129 22.42 25.86 24.7 27.02 130-134 22.74 24.865000000000002 24.875 27.52 135-139 22.85 25.355 25.765 26.029999999999998 140-144 23.51 25.52 24.985 25.985000000000003 145-149 22.314999999999998 25.740000000000002 25.624999999999996 26.32 150-151 22.098409916113685 25.679228746713413 25.616627018905724 26.60573431826718 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.5 20 3.0 21 4.0 22 4.0 23 2.5 24 5.0 25 8.5 26 6.5 27 7.0 28 15.5 29 17.5 30 17.5 31 22.0 32 21.0 33 21.5 34 30.0 35 47.0 36 69.5 37 104.0 38 118.5 39 111.0 40 122.5 41 136.0 42 135.5 43 145.5 44 140.0 45 120.0 46 106.0 47 98.0 48 92.5 49 90.5 50 101.5 51 122.0 52 143.5 53 163.0 54 196.5 55 248.0 56 271.0 57 231.5 58 196.0 59 159.0 60 109.5 61 67.5 62 43.0 63 41.5 64 33.5 65 18.5 66 10.5 67 6.0 68 2.5 69 2.0 70 2.0 71 1.5 72 1.0 73 0.5 74 0.5 75 1.0 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.1625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.95 #Duplication Level Percentage of deduplicated Percentage of total 1 85.19012675116745 63.849999999999994 2 8.539026017344895 12.8 3 2.66844563042028 6.0 4 1.1674449633088726 3.5000000000000004 5 0.7671781187458305 2.875 6 0.5336891260840559 2.4 7 0.26684456304202797 1.4000000000000001 8 0.20013342228152103 1.2 9 0.10006671114076052 0.675 >10 0.5670446964643095 5.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 21 0.525 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 15 0.375 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 15 0.375 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 14 0.35000000000000003 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 14 0.35000000000000003 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 14 0.35000000000000003 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 13 0.325 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 12 0.3 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 12 0.3 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 11 0.27499999999999997 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 11 0.27499999999999997 No Hit CACCTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTA 10 0.25 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 10 0.25 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 10 0.25 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 10 0.25 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 10 0.25 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 10 0.25 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 9 0.22499999999999998 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 9 0.22499999999999998 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 9 0.22499999999999998 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 8 0.2 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 8 0.2 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 8 0.2 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 8 0.2 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 8 0.2 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 8 0.2 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 7 0.17500000000000002 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 7 0.17500000000000002 No Hit CCCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTC 7 0.17500000000000002 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 7 0.17500000000000002 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 7 0.17500000000000002 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 7 0.17500000000000002 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 7 0.17500000000000002 No Hit GCCCATTGTAGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTG 7 0.17500000000000002 No Hit CACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCCTC 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit GGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTT 6 0.15 No Hit GTGGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACAC 6 0.15 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 6 0.15 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 6 0.15 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 6 0.15 No Hit CCTAGGTATTCTCTACCTACCCACCTGTGTCGGTTTCGGGTACAGGTACC 6 0.15 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 6 0.15 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 6 0.15 No Hit GCCAGGTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAAAGGT 6 0.15 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 6 0.15 No Hit CATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCT 6 0.15 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 6 0.15 No Hit GCCCCTCCTTGGGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATT 6 0.15 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 5 0.125 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 5 0.125 No Hit ATAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCGCGGGTCTGACACAA 5 0.125 No Hit CTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCC 5 0.125 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 5 0.125 No Hit CCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCC 5 0.125 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 5 0.125 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 5 0.125 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 5 0.125 No Hit CCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGG 5 0.125 No Hit CTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCC 5 0.125 No Hit GTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGC 5 0.125 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit CCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTAT 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit CCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTTCAACAGGCA 5 0.125 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 5 0.125 No Hit GGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTG 5 0.125 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 5 0.125 No Hit GCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAG 5 0.125 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 5 0.125 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.0875 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1125 0.0 0.0 0.0 0.0 62-63 0.1375 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.21250000000000002 0.0 0.0 0.0 0.0 72-73 0.2375 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.36250000000000004 0.0 0.0 0.0 0.0 78-79 0.475 0.0 0.0 0.0 0.0 80-81 0.525 0.0 0.0 0.0 0.0 82-83 0.6625000000000001 0.0 0.0 0.0 0.0 84-85 0.75 0.0 0.0 0.0 0.0 86-87 0.975 0.0 0.0 0.0 0.0 88-89 1.15 0.0 0.0 0.0 0.0 90-91 1.375 0.0 0.0 0.0 0.0 92-93 1.6124999999999998 0.0 0.0 0.0 0.0 94-95 1.9 0.0 0.0 0.0 0.0 96-97 2.1625 0.0 0.0 0.0 0.0 98-99 2.4875 0.0 0.0 0.0 0.0 100-101 2.925 0.0 0.0 0.0 0.0 102-103 3.2625 0.0 0.0 0.0 0.0 104-105 3.7625 0.0 0.0 0.0 0.0 106-107 4.262499999999999 0.0 0.0 0.0 0.0 108-109 4.725 0.0 0.0 0.0 0.0 110-111 5.300000000000001 0.0 0.0 0.0 0.0 112-113 5.7875 0.0 0.0 0.0 0.0 114-115 6.35 0.0 0.0 0.0 0.0 116-117 6.9125 0.0 0.0 0.0 0.0 118-119 7.3875 0.0 0.0 0.0 0.0 120-121 7.7625 0.0 0.0 0.0 0.0 122-123 8.2375 0.0 0.0 0.0 0.0 124-125 8.675 0.0 0.0 0.0 0.0 126-127 9.287500000000001 0.0 0.0 0.0 0.0 128-129 9.7625 0.0 0.0 0.0 0.0 130-131 10.4125 0.0 0.0 0.0 0.0 132-133 10.975 0.0 0.0 0.0 0.0 134-135 11.525 0.0 0.0 0.0 0.0 136-137 12.175 0.0 0.0 0.0 0.0 138-139 13.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGCATA 35 0.003315817 62.12679 145 >>END_MODULE SRR6257529 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6257529_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.031 33.0 32.0 33.0 28.0 34.0 2 32.41825 33.0 33.0 34.0 32.0 34.0 3 32.609 33.0 33.0 34.0 32.0 34.0 4 32.765 33.0 33.0 34.0 32.0 34.0 5 28.67925 33.0 27.0 33.0 15.0 34.0 6 35.564 38.0 36.0 38.0 30.0 38.0 7 36.45 38.0 37.0 38.0 34.0 38.0 8 36.95275 38.0 38.0 38.0 36.0 38.0 9 36.96825 38.0 38.0 38.0 36.0 38.0 10-14 34.9225 37.4 33.8 38.0 29.0 38.0 15-19 35.2373 38.0 35.0 38.0 25.6 38.0 20-24 37.100049999999996 38.0 38.0 38.0 36.4 38.0 25-29 37.11280000000001 38.0 38.0 38.0 36.6 38.0 30-34 36.7636 38.0 37.8 38.0 34.8 38.0 35-39 37.05965 38.0 38.0 38.0 36.0 38.0 40-44 37.100449999999995 38.0 38.0 38.0 36.4 38.0 45-49 36.83605 38.0 38.0 38.0 35.4 38.0 50-54 36.76835 38.0 38.0 38.0 35.2 38.0 55-59 36.85455 38.0 38.0 38.0 35.6 38.0 60-64 35.56955 38.0 35.6 38.0 29.6 38.0 65-69 36.242149999999995 38.0 37.4 38.0 32.8 38.0 70-74 36.6961 38.0 38.0 38.0 34.6 38.0 75-79 36.17315 38.0 37.2 38.0 32.2 38.0 80-84 36.498850000000004 38.0 38.0 38.0 34.4 38.0 85-89 35.79110000000001 38.0 36.8 38.0 29.6 38.0 90-94 36.117650000000005 38.0 37.4 38.0 33.2 38.0 95-99 32.2767 35.4 28.0 38.0 23.2 38.0 100-104 35.16885 37.8 35.2 38.0 29.6 38.0 105-109 35.9657 38.0 36.8 38.0 32.8 38.0 110-114 35.481 38.0 36.0 38.0 30.6 38.0 115-119 35.349450000000004 38.0 36.0 38.0 30.2 38.0 120-124 35.150850000000005 38.0 35.2 38.0 29.6 38.0 125-129 33.939249999999994 38.0 33.8 38.0 23.8 38.0 130-134 31.38945 35.6 28.0 38.0 16.8 38.0 135-139 33.045199999999994 37.6 32.6 38.0 20.4 38.0 140-144 32.68385 38.0 32.6 38.0 17.2 38.0 145-149 31.56615 38.0 31.2 38.0 8.4 38.0 150-151 25.941000000000003 33.0 16.0 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 1.0 5 0.0 6 0.0 7 2.0 8 1.0 9 2.0 10 2.0 11 1.0 12 1.0 13 0.0 14 2.0 15 0.0 16 6.0 17 5.0 18 4.0 19 10.0 20 9.0 21 5.0 22 10.0 23 14.0 24 20.0 25 24.0 26 22.0 27 27.0 28 36.0 29 49.0 30 72.0 31 89.0 32 136.0 33 191.0 34 329.0 35 616.0 36 1262.0 37 1048.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.975 17.775 11.35 28.9 2 32.875 20.349999999999998 28.849999999999998 17.925 3 23.400000000000002 23.775 32.074999999999996 20.75 4 25.55 31.4 24.575 18.475 5 29.099999999999998 32.925 19.925 18.05 6 25.1 35.825 19.900000000000002 19.175 7 22.2 21.55 35.25 21.0 8 25.275 23.05 25.275 26.400000000000002 9 26.25 22.475 27.900000000000002 23.375 10-14 26.865 26.61 24.59 21.935 15-19 27.32 25.735000000000003 25.165 21.78 20-24 26.540000000000003 25.995 25.629999999999995 21.834999999999997 25-29 26.962696269626964 26.227622762276226 25.027502750275026 21.782178217821784 30-34 27.011350567528375 26.161308065403272 24.846242312115603 21.981099054952747 35-39 27.431371568578427 26.781339066953347 25.016250812540626 20.771038551927596 40-44 26.950000000000003 26.575 25.014999999999997 21.46 45-49 26.505000000000003 27.365000000000002 24.495 21.634999999999998 50-54 27.450000000000003 25.759999999999998 25.45 21.34 55-59 26.200000000000003 27.235 24.265 22.3 60-64 26.555 25.69 25.790000000000003 21.965 65-69 27.839999999999996 26.279999999999998 24.69 21.19 70-74 28.015 25.759999999999998 25.490000000000002 20.735 75-79 27.47 25.52 24.905 22.105 80-84 26.669999999999998 26.064999999999998 25.96 21.305 85-89 27.365000000000002 26.279999999999998 24.505 21.85 90-94 26.89134456722836 26.401320066003297 24.686234311715584 22.021101055052753 95-99 27.235 26.3 24.38 22.085 100-104 27.24 27.605 24.065 21.09 105-109 28.27 25.650000000000002 25.259999999999998 20.82 110-114 28.005000000000003 26.16 25.0 20.835 115-119 27.46 26.44 25.485000000000003 20.615 120-124 27.555000000000003 26.545 23.97 21.93 125-129 28.025 26.705000000000002 24.16 21.11 130-134 27.715 26.479999999999997 24.36 21.445 135-139 27.615000000000002 26.284999999999997 24.85 21.25 140-144 28.585 26.06 24.990000000000002 20.365 145-149 28.660000000000004 26.240000000000002 24.89 20.21 150-151 28.256513026052104 25.576152304609217 25.225450901803608 20.941883767535067 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 1.0 21 2.5 22 3.0 23 2.0 24 2.0 25 3.5 26 9.5 27 14.5 28 15.5 29 17.0 30 21.0 31 25.5 32 26.5 33 33.5 34 51.0 35 62.5 36 65.5 37 79.0 38 92.0 39 110.0 40 128.0 41 129.0 42 134.0 43 135.0 44 122.0 45 117.5 46 118.5 47 98.5 48 93.5 49 106.5 50 111.0 51 121.5 52 128.5 53 159.0 54 222.5 55 255.0 56 235.0 57 192.0 58 169.0 59 158.5 60 129.0 61 99.0 62 70.5 63 43.0 64 25.5 65 17.5 66 13.0 67 9.0 68 7.5 69 5.5 70 3.5 71 1.5 72 0.5 73 1.5 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.01 30-34 0.005 35-39 0.005 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.2 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.65 #Duplication Level Percentage of deduplicated Percentage of total 1 90.28021015761821 77.325 2 6.509048453006422 11.15 3 1.926444833625219 4.95 4 0.642148277875073 2.1999999999999997 5 0.3502626970227671 1.5 6 0.02918855808523059 0.15 7 0.02918855808523059 0.17500000000000002 8 0.05837711617046118 0.4 9 0.02918855808523059 0.22499999999999998 >10 0.14594279042615294 1.925 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 27 0.675 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 15 0.375 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 15 0.375 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 10 0.25 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 10 0.25 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 9 0.22499999999999998 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 8 0.2 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 8 0.2 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 7 0.17500000000000002 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 5 0.125 No Hit CGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTC 5 0.125 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 5 0.125 No Hit GAAAATGCCTCGAGCCGAGGTCCGAGTACCAAGCGCTGCAGCGCTGAAGT 5 0.125 No Hit GGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGG 5 0.125 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 5 0.125 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 5 0.125 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 5 0.125 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.0875 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1125 0.0 0.0 0.0 0.0 62-63 0.1375 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.21250000000000002 0.0 0.0 0.0 0.0 72-73 0.2375 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.36250000000000004 0.0 0.0 0.0 0.0 78-79 0.475 0.0 0.0 0.0 0.0 80-81 0.5375 0.0 0.0 0.0 0.0 82-83 0.6625000000000001 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.9375 0.0 0.0 0.0 0.0 88-89 1.0625 0.0 0.0 0.0 0.0 90-91 1.225 0.0 0.0 0.0 0.0 92-93 1.375 0.0 0.0 0.0 0.0 94-95 1.625 0.0 0.0 0.0 0.0 96-97 1.8624999999999998 0.0 0.0 0.0 0.0 98-99 2.2 0.0 0.0 0.0 0.0 100-101 2.6500000000000004 0.0 0.0 0.0 0.0 102-103 2.9875 0.0 0.0 0.0 0.0 104-105 3.5 0.0 0.0 0.0 0.0 106-107 4.0125 0.0 0.0 0.0 0.0 108-109 4.5 0.0 0.0 0.0 0.0 110-111 5.074999999999999 0.0 0.0 0.0 0.0 112-113 5.5625 0.0 0.0 0.0 0.0 114-115 6.15 0.0 0.0 0.0 0.0 116-117 6.6625 0.0 0.0 0.0 0.0 118-119 7.1 0.0 0.0 0.0 0.0 120-121 7.425 0.0 0.0 0.0 0.0 122-123 7.887499999999999 0.0 0.0 0.0 0.0 124-125 8.3125 0.0 0.0 0.0 0.0 126-127 8.9625 0.0 0.0 0.0 0.0 128-129 9.475 0.0 0.0 0.0 0.0 130-131 10.125 0.0 0.0 0.0 0.0 132-133 10.712499999999999 0.0 0.0 0.0 0.0 134-135 11.3125 0.0 0.0 0.0 0.0 136-137 12.0625 0.0 0.0 0.0 0.0 138-139 12.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTTCAA 10 0.006830828 145.0 145 GGAAATT 10 0.006830828 145.0 1 >>END_MODULE Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674062 spots for SRR6257529.sra Written 674062 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra Read 674057 spots for SRR6257529.sra Written 674057 spots for SRR6257529.sra SRR ids: ['SRR6257529.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jl3j_7e4 SRR6257529.sra spots: 13481145 blocks: [[1, 674057], [674058, 1348114], [1348115, 2022171], [2022172, 2696228], [2696229, 3370285], [3370286, 4044342], [4044343, 4718399], [4718400, 5392456], [5392457, 6066513], [6066514, 6740570], [6740571, 7414627], [7414628, 8088684], [8088685, 8762741], [8762742, 9436798], [9436799, 10110855], [10110856, 10784912], [10784913, 11458969], [11458970, 12133026], [12133027, 12807083], [12807084, 13481145]] SRR6257529 file size 4546617 SRR6257529 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6257529 SRR6257529_1.fastq SRR6257529_2.fastq Input file: SRR6257529_1.fastq Paired file: SRR6257529_2.fastq trimmed: SRR6257529-trimmed-pair1.fastq, SRR6257529-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 09:06:38 2024 >> started Sat Dec 7 09:06:52 2024 >> done (14.246s) 13481145 read pairs processed; of these: 8269 ( 0.06%) short read pairs filtered out after trimming by size control 12354 ( 0.09%) empty read pairs filtered out after trimming by size control 13460522 (99.85%) read pairs available; of these: 7759898 (57.65%) trimmed read pairs available after processing 5700624 (42.35%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 3 0.00% 20 1 0.00% 21 5 0.00% 22 4 0.00% 23 4 0.00% 24 4 0.00% 25 6 0.00% 26 14 0.00% 27 23 0.00% 28 22 0.00% 29 25 0.00% 30 35 0.00% 31 48 0.00% 32 57 0.00% 33 54 0.00% 34 51 0.00% 35 55 0.00% 36 70 0.00% 37 69 0.00% 38 78 0.00% 39 151 0.00% 40 152 0.00% 41 147 0.00% 42 158 0.00% 43 171 0.00% 44 194 0.00% 45 166 0.00% 46 206 0.00% 47 249 0.00% 48 283 0.00% 49 341 0.00% 50 400 0.00% 51 430 0.00% 52 488 0.00% 53 458 0.00% 54 570 0.00% 55 578 0.00% 56 621 0.00% 57 704 0.01% 58 788 0.01% 59 992 0.01% 60 1100 0.01% 61 1320 0.01% 62 1429 0.01% 63 1605 0.01% 64 1777 0.01% 65 1917 0.01% 66 1956 0.01% 67 2293 0.02% 68 2542 0.02% 69 2975 0.02% 70 3269 0.02% 71 3460 0.03% 72 4128 0.03% 73 4420 0.03% 74 4444 0.03% 75 4857 0.04% 76 5206 0.04% 77 5279 0.04% 78 5560 0.04% 79 6534 0.05% 80 7272 0.05% 81 7956 0.06% 82 8573 0.06% 83 9572 0.07% 84 10263 0.08% 85 12394 0.09% 86 12272 0.09% 87 12849 0.10% 88 14583 0.11% 89 14096 0.10% 90 14873 0.11% 91 15974 0.12% 92 18264 0.14% 93 19791 0.15% 94 20552 0.15% 95 21786 0.16% 96 21889 0.16% 97 23603 0.18% 98 23864 0.18% 99 25003 0.19% 100 25878 0.19% 101 28012 0.21% 102 30517 0.23% 103 30067 0.22% 104 33564 0.25% 105 32383 0.24% 106 36025 0.27% 107 36838 0.27% 108 36635 0.27% 109 40566 0.30% 110 38889 0.29% 111 40287 0.30% 112 42526 0.32% 113 37884 0.28% 114 40731 0.30% 115 41968 0.31% 116 42631 0.32% 117 41719 0.31% 118 40663 0.30% 119 41284 0.31% 120 44040 0.33% 121 44884 0.33% 122 46664 0.35% 123 50541 0.38% 124 50191 0.37% 125 53283 0.40% 126 52495 0.39% 127 53306 0.40% 128 53776 0.40% 129 55568 0.41% 130 56031 0.42% 131 58975 0.44% 132 59169 0.44% 133 58825 0.44% 134 62420 0.46% 135 64005 0.48% 136 69448 0.52% 137 70792 0.53% 138 74695 0.55% 139 80546 0.60% 140 83310 0.62% 141 92687 0.69% 142 99784 0.74% 143 114011 0.85% 144 128951 0.96% 145 156381 1.16% 146 191755 1.42% 147 250205 1.86% 148 377250 2.80% 149 740369 5.50% 150 3236093 24.04% 151 5700624 42.35% 13460522 reads passed initial QC criterion=sequence-density sequence-density=0.86 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=36 prefix-density=0.86 prefix-fanout=1.9 sequence=TGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=205.61 fanout-score-rank=1 prefix-density=1.32 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=30 prefix-density=0.54 prefix-fanout=2.0 sequence=GCGTGAGGCTGG criterion=fanout-score sequence-density=0.03 sequence-density-rank=29 fanout-score=65.16 fanout-score-rank=1 prefix-density=1.88 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6257529 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 09:07:25 Started mapping on | Dec 07 09:07:25 Finished on | Dec 07 09:08:31 Mapping speed, Million of reads per hour | 734.21 Number of input reads | 13460522 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 6583947 Uniquely mapped reads % | 48.91% Average mapped length | 292.05 Number of splices: Total | 1105357 Number of splices: Annotated (sjdb) | 994040 Number of splices: GT/AG | 1047366 Number of splices: GC/AG | 13623 Number of splices: AT/AC | 3603 Number of splices: Non-canonical | 40765 Mismatch rate per base, % | 0.19% Deletion rate per base | 0.01% Deletion average length | 1.70 Insertion rate per base | 0.01% Insertion average length | 1.94 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 5784359 % of reads mapped to multiple loci | 42.97% Number of reads mapped to too many loci | 120685 % of reads mapped to too many loci | 0.90% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.51% % of reads unmapped: other | 3.71% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1096260 1096260 1096260 N_multimapping 5784359 5784359 5784359 N_noFeature 3777625 6415947 3842242 N_ambiguous 230089 2654 131755 UnstrandedReadsAssigned:2576233 PositiveStrandReadsAssigned:165346 NegativeStrandReadsAssigned:2609950 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR6257529 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6257529-trimmed-pair1.fastq SRR6257529-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 13,460,522 reads, 5,099,872 reads pseudoaligned [quant] estimated average fragment length: 204.387 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 921 rounds 52973 SRR6257529.ke.tsv 35125 SRR6257529.se.tsv 88098 total ==> SRR6257529.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 733.381 0 0 PNS24247 1044 840.613 4.08636 0.547522 PNS24249 1928 1724.61 0 0 PNS24246 1044 840.613 4.08636 0.547522 PNS24248 1044 840.613 4.08636 0.547522 PNS24244 1471 1267.61 6.74093 0.598955 PNS24243 293 114.653 0 0 KQK14069 1603 1399.61 307.311 24.7304 KQK14071 474 276.287 7.40461 3.01858 ==> SRR6257529.se.tsv <== BRADI_1g14170v3 432 BRADI_1g53295v3 1 BRADI_1g59795v3 13 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 151 BRADI_1g74790v3 6 BRADI_1g09890v3 1 BRADI_1g77505v3 32 BRADI_1g48960v3 0 SRR6257529 completed mapping pipeline successfully