Starting /dee2/code/volunteer_pipeline.sh SRR6322407 current disk space = 1543573643264 free memory = 1418223504 SRR6322407 SRAfilesize 9b8a5622e92c7875859f6d06f0f04461 SRR6322407.sra SRR6322407.sra file validated SRR6322407 is single end SRR6322407 is conventional basespace SRR6322407 read1 length is 50 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6322407_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 50 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41375 33.0 33.0 34.0 32.0 34.0 2 32.5555 34.0 33.0 34.0 31.0 34.0 3 32.61575 34.0 33.0 34.0 31.0 34.0 4 32.49575 34.0 33.0 34.0 31.0 34.0 5 32.51 34.0 33.0 34.0 31.0 34.0 6 36.068 38.0 36.0 38.0 31.0 38.0 7 36.5145 38.0 37.0 38.0 34.0 38.0 8 36.66475 38.0 38.0 38.0 34.0 38.0 9 36.608 38.0 38.0 38.0 34.0 38.0 10 36.7305 38.0 38.0 38.0 34.0 38.0 11 36.76125 38.0 38.0 38.0 34.0 38.0 12 36.72975 38.0 38.0 38.0 34.0 38.0 13 36.71925 38.0 38.0 38.0 34.0 38.0 14 36.74725 38.0 38.0 38.0 34.0 38.0 15 36.809 38.0 38.0 38.0 35.0 38.0 16 36.67775 38.0 38.0 38.0 34.0 38.0 17 36.705 38.0 38.0 38.0 34.0 38.0 18 36.6405 38.0 38.0 38.0 34.0 38.0 19 36.78525 38.0 38.0 38.0 34.0 38.0 20 36.76975 38.0 38.0 38.0 35.0 38.0 21 36.67375 38.0 38.0 38.0 34.0 38.0 22 36.72925 38.0 38.0 38.0 34.0 38.0 23 36.783 38.0 38.0 38.0 34.0 38.0 24 36.7565 38.0 38.0 38.0 35.0 38.0 25 36.7245 38.0 38.0 38.0 35.0 38.0 26 36.6405 38.0 38.0 38.0 34.0 38.0 27 36.6975 38.0 38.0 38.0 34.0 38.0 28 36.60225 38.0 38.0 38.0 34.0 38.0 29 36.66575 38.0 38.0 38.0 34.0 38.0 30 36.65675 38.0 38.0 38.0 34.0 38.0 31 36.61375 38.0 38.0 38.0 34.0 38.0 32 36.67325 38.0 38.0 38.0 34.0 38.0 33 36.60925 38.0 38.0 38.0 34.0 38.0 34 36.581 38.0 38.0 38.0 34.0 38.0 35 36.6115 38.0 38.0 38.0 34.0 38.0 36 36.62525 38.0 38.0 38.0 34.0 38.0 37 36.5925 38.0 38.0 38.0 34.0 38.0 38 36.568 38.0 38.0 38.0 34.0 38.0 39 36.54825 38.0 38.0 38.0 34.0 38.0 40 36.532 38.0 38.0 38.0 34.0 38.0 41 36.69425 38.0 38.0 38.0 34.0 38.0 42 36.5885 38.0 38.0 38.0 35.0 38.0 43 36.5345 38.0 38.0 38.0 34.0 38.0 44 36.4625 38.0 38.0 38.0 34.0 38.0 45 36.42575 38.0 38.0 38.0 34.0 38.0 46 36.52575 38.0 38.0 38.0 34.0 38.0 47 36.418 38.0 38.0 38.0 34.0 38.0 48 36.47375 38.0 38.0 38.0 34.0 38.0 49 36.37275 38.0 38.0 38.0 34.0 38.0 50 36.35975 38.0 38.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10 0.0 1101 11 0.0 1101 12 0.0 1101 13 0.0 1101 14 0.0 1101 15 0.0 1101 16 0.0 1101 17 0.0 1101 18 0.0 1101 19 0.0 1101 20 0.0 1101 21 0.0 1101 22 0.0 1101 23 0.0 1101 24 0.0 1101 25 0.0 1101 26 0.0 1101 27 0.0 1101 28 0.0 1101 29 0.0 1101 30 0.0 1101 31 0.0 1101 32 0.0 1101 33 0.0 1101 34 0.0 1101 35 0.0 1101 36 0.0 1101 37 0.0 1101 38 0.0 1101 39 0.0 1101 40 0.0 1101 41 0.0 1101 42 0.0 1101 43 0.0 1101 44 0.0 1101 45 0.0 1101 46 0.0 1101 47 0.0 1101 48 0.0 1101 49 0.0 1101 50 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 2.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 2.0 22 3.0 23 7.0 24 7.0 25 7.0 26 19.0 27 27.0 28 46.0 29 43.0 30 70.0 31 67.0 32 97.0 33 119.0 34 177.0 35 273.0 36 513.0 37 2517.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.80070546737213 14.865205341395818 7.961703199798438 38.37238599143361 2 25.45 19.525000000000002 34.150000000000006 20.875 3 21.8 23.325000000000003 24.3 30.575000000000003 4 26.650000000000002 29.775000000000002 19.55 24.025 5 24.88744372186093 31.615807903951975 21.860930465232617 21.635817908954476 6 19.900000000000002 30.575000000000003 22.55 26.974999999999998 7 18.575 16.85 38.224999999999994 26.35 8 21.25 18.05 26.525 34.175 9 21.075 17.724999999999998 29.299999999999997 31.900000000000002 10 23.25 32.75 20.3 23.7 11 28.825 20.25 18.375 32.550000000000004 12 28.475 16.35 21.625 33.550000000000004 13 23.375 23.05 24.775 28.799999999999997 14 24.625 24.425 25.650000000000002 25.3 15 23.799999999999997 22.75 25.224999999999998 28.225 16 24.099999999999998 23.150000000000002 24.4 28.349999999999998 17 23.825 23.150000000000002 25.424999999999997 27.6 18 23.599999999999998 23.849999999999998 25.674999999999997 26.875 19 25.5 24.275 23.525 26.700000000000003 20 24.95 22.375 25.324999999999996 27.35 21 24.625 23.775 24.175 27.425 22 24.125 24.224999999999998 24.75 26.900000000000002 23 25.05 24.4 23.674999999999997 26.875 24 23.35 22.525000000000002 25.474999999999998 28.65 25 24.2 23.799999999999997 23.25 28.749999999999996 26 24.05 23.7 25.7 26.55 27 23.7 24.099999999999998 24.65 27.55 28 25.2 23.575 23.525 27.700000000000003 29 24.0 23.825 25.224999999999998 26.950000000000003 30 25.1 23.625 24.099999999999998 27.175 31 23.1 23.125 24.325 29.45 32 24.775 24.099999999999998 25.124999999999996 26.0 33 23.474999999999998 23.075000000000003 25.974999999999998 27.474999999999998 34 24.05 24.6 22.675 28.675 35 22.575 25.0 25.424999999999997 27.0 36 24.3 23.599999999999998 23.9 28.199999999999996 37 23.925 22.85 25.1 28.125 38 23.5 23.45 25.124999999999996 27.925 39 23.7 22.975 24.9 28.425 40 23.080770192548137 23.20580145036259 24.731182795698924 28.982245561390346 41 24.125 24.075 24.65 27.150000000000002 42 22.45 24.175 25.8 27.575 43 25.424999999999997 23.200000000000003 24.975 26.400000000000002 44 23.1 24.125 25.775 27.0 45 22.95 23.549999999999997 26.05 27.450000000000003 46 24.075 22.8 25.4 27.725 47 23.35 23.35 25.474999999999998 27.825 48 22.425 23.825 26.325 27.425 49 25.874999999999996 23.25 23.575 27.3 50 23.125 22.775000000000002 25.2 28.9 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 2.0 22 2.0 23 2.5 24 3.0 25 7.0 26 11.0 27 12.0 28 13.0 29 19.5 30 26.0 31 37.5 32 49.0 33 53.0 34 57.0 35 82.0 36 107.0 37 127.5 38 148.0 39 167.0 40 186.0 41 213.5 42 241.0 43 261.5 44 282.0 45 284.0 46 286.0 47 285.5 48 285.0 49 299.5 50 314.0 51 301.5 52 289.0 53 277.5 54 266.0 55 239.0 56 212.0 57 212.0 58 212.0 59 186.5 60 161.0 61 161.0 62 161.0 63 156.0 64 151.0 65 131.5 66 112.0 67 103.5 68 95.0 69 94.5 70 94.0 71 77.0 72 60.0 73 54.5 74 49.0 75 49.0 76 49.0 77 43.0 78 37.0 79 30.0 80 23.0 81 17.0 82 11.0 83 6.5 84 2.0 85 3.0 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.775 2 0.0 3 0.0 4 0.0 5 0.05 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.025 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.35 #Duplication Level Percentage of deduplicated Percentage of total 1 94.07858328721638 85.0 2 3.7354731599335915 6.75 3 1.0514665190924184 2.85 4 0.44272274488101826 1.6 5 0.3597122302158274 1.625 6 0.16602102933038185 0.8999999999999999 7 0.08301051466519092 0.525 8 0.02767017155506364 0.2 9 0.02767017155506364 0.22499999999999998 >10 0.02767017155506364 0.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 13 0.325 No Hit CAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCT 9 0.22499999999999998 No Hit GTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTC 8 0.2 No Hit CTCCGATCCCGAAGGCCAACACAATAGGACCGGAATCCTATGATGTTATC 7 0.17500000000000002 No Hit GCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGTCAGA 7 0.17500000000000002 No Hit GTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAA 7 0.17500000000000002 No Hit GACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTT 6 0.15 No Hit CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC 6 0.15 No Hit AGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGC 6 0.15 No Hit GTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGAC 6 0.15 No Hit CGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGCATAGTTC 6 0.15 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 6 0.15 No Hit ATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTCGAGTTAT 5 0.125 No Hit CAGGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGGCGTC 5 0.125 No Hit TAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTG 5 0.125 No Hit CAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTAC 5 0.125 No Hit CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGT 5 0.125 No Hit CTTTTATCTAATAAATGCGCCCCTCCCAGAAGTCGGGGTTTGTTGCACGT 5 0.125 No Hit CCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACAT 5 0.125 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 5 0.125 No Hit CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT 5 0.125 No Hit CTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 5 0.125 No Hit GAACGATTTGCACGTCAGTATCGCTTCGAGCCTCCACCAGAGTTTCCTCT 5 0.125 No Hit CGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCGGCCA 5 0.125 No Hit GGGGAAGGGAGCTTCGAGGCGGCCGGACGCGGCTCGTCGGCCGGAACGGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324432 spots for SRR6322407.sra Written 1324432 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra Read 1324426 spots for SRR6322407.sra Written 1324426 spots for SRR6322407.sra SRR ids: ['SRR6322407.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7a1nbby6 SRR6322407.sra spots: 26488526 blocks: [[1, 1324426], [1324427, 2648852], [2648853, 3973278], [3973279, 5297704], [5297705, 6622130], [6622131, 7946556], [7946557, 9270982], [9270983, 10595408], [10595409, 11919834], [11919835, 13244260], [13244261, 14568686], [14568687, 15893112], [15893113, 17217538], [17217539, 18541964], [18541965, 19866390], [19866391, 21190816], [21190817, 22515242], [22515243, 23839668], [23839669, 25164094], [25164095, 26488526]] SRR6322407 file size 4606259 SRR6322407 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322407 SRR6322407_1.fastq Input file: SRR6322407_1.fastq trimmed: SRR6322407-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 10:26:38 2024 >> started Sat Dec 7 10:27:46 2024 >> done (67.509s) 26488526 reads processed; of these: 6323 ( 0.02%) short reads filtered out after trimming by size control 21135 ( 0.08%) empty reads filtered out after trimming by size control 26461068 (99.90%) reads available; of these: 414015 ( 1.56%) trimmed reads available after processing 26047053 (98.44%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 384 0.00% 19 399 0.00% 20 492 0.00% 21 581 0.00% 22 618 0.00% 23 773 0.00% 24 942 0.00% 25 1271 0.00% 26 1281 0.00% 27 1283 0.00% 28 1414 0.01% 29 1513 0.01% 30 1683 0.01% 31 1927 0.01% 32 2187 0.01% 33 2462 0.01% 34 2881 0.01% 35 3350 0.01% 36 3931 0.01% 37 4518 0.02% 38 5583 0.02% 39 6453 0.02% 40 7948 0.03% 41 9638 0.04% 42 12553 0.05% 43 15155 0.06% 44 19315 0.07% 45 25858 0.10% 46 36453 0.14% 47 49469 0.19% 48 78180 0.30% 49 113520 0.43% 50 26047053 98.44% 26461068 reads passed initial QC criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=3.19 fanout-score-rank=26 prefix-density=0.20 prefix-fanout=3.1 sequence=TCCTGCTGCTGCT criterion=fanout-score sequence-density=0.02 sequence-density-rank=25 fanout-score=114.01 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=10.8 sequence=CCGCCGCCGCCTCCTCCGCCACGACCGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCAC Started job on | Dec 07 10:29:29 Started mapping on | Dec 07 10:29:29 Finished on | Dec 07 10:34:31 Mapping speed, Million of reads per hour | 315.43 Number of input reads | 26461068 Average input read length | 49 UNIQUE READS: Uniquely mapped reads number | 18095304 Uniquely mapped reads % | 68.38% Average mapped length | 49.82 Number of splices: Total | 2577403 Number of splices: Annotated (sjdb) | 2468488 Number of splices: GT/AG | 2540773 Number of splices: GC/AG | 29727 Number of splices: AT/AC | 1784 Number of splices: Non-canonical | 5119 Mismatch rate per base, % | 0.19% Deletion rate per base | 0.01% Deletion average length | 1.63 Insertion rate per base | 0.00% Insertion average length | 1.53 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 714082 % of reads mapped to multiple loci | 2.70% Number of reads mapped to too many loci | 7342797 % of reads mapped to too many loci | 27.75% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.81% % of reads unmapped: other | 0.36% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 7651682 7651682 7651682 N_multimapping 714082 714082 714082 N_noFeature 1125751 17670508 1382486 N_ambiguous 188037 1020 19757 UnstrandedReadsAssigned:16781516 PositiveStrandReadsAssigned:423776 NegativeStrandReadsAssigned:16693061 Dataset is classified negative stranded MeadianReadLen=50 20thPercentileLength=50 echo kmer=45 SRR6322407 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR6322407-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,461,068 reads, 16,737,302 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,200 rounds 52973 SRR6322407.ke.tsv 35125 SRR6322407.se.tsv 88098 total ==> SRR6322407.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 124.329 14.1119 PNS24247 1044 945 40.2912 4.05059 PNS24249 1928 1829 188.018 9.76621 PNS24246 1044 945 40.2912 4.05059 PNS24248 1044 945 40.2912 4.05059 PNS24244 1471 1372 69.7797 4.83187 PNS24243 293 194 0 0 KQK14069 1603 1504 156.234 9.86888 KQK14071 474 375 27.8741 7.06172 ==> SRR6322407.se.tsv <== BRADI_1g14170v3 192 BRADI_1g53295v3 1341 BRADI_1g59795v3 517 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 509 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 193 BRADI_1g48960v3 1 SRR6322407 completed mapping pipeline successfully